itk cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	itk
Entrez Gene ID	798906
Full Name	IL2 inducible T cell kinase
Synonyms	Ptk,tec
General protein information	Preferred Names IL2 inducible T cell kinase Names tyrosine-protein kinase ITK/TSK tec-family kinase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131104.1	NP_571179.1	tyrosine-protein kinase ITK/TSK

Pathways from BioSystems

Homologies

Homo sapiens (human)	ITK	NP_005537.3
Pan troglodytes (chimpanzee)	ITK	XP_001136073.1
Bos taurus (cattle)	ITK	NP_001098858.1
Canis lupus familiaris (dog)	ITK	XP_546277.3
Mus musculus (house mouse)	Itk	NP_001268894.1
	ITK	XP_001113468.1
Danio rerio (zebrafish)	itk	NP_571179.1
Rattus norvegicus (Norway rat)	Itk	NP_001102295.1
Gallus gallus (chicken)	ITK	XP_414568.3

Gene Ontology
Bibliography

Related articles in PubMed

Apela Regulates Fluid Homeostasis by Binding to the APJ Receptor to Activate Gi Signaling.
Deng C, Chen H, Yang N, Feng Y, Hsueh AJ
The Journal of biological chemistry290(30)18261-8(2015 Jul)

A genetic linkage map for zebrafish: comparative analysis and localization of genes and expressed sequences.
Gates MA, Kim L, Egan ES, Cardozo T, Sirotkin HI, Dougan ST, Lashkari D, Abagyan R, Schier AF, Talbot WS
Genome research9(4)334-47(1999 Apr)

Zebrafish expressed sequence tags and their applications.
Gong Z
Methods in cell biology60213-33(1999)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following itk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the itk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35673	NM_131104.1 Latest version!	Danio rerio IL2 inducible T cell kinase (itk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

itk ( NM_131104.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571179.1 Danio rerio IL2 inducible T cell kinase (itk), mRNA.

Price & Availability↑

CloneID	ODa35673
Clone ID Related Accession (Same CDS sequence)	NM_131104.1
Accession Version	NM_131104.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-12
Organism	Danio rerio(zebrafish)
Product	tyrosine-protein kinase ITK/TSK
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF016326.1. On or before Aug 31, 2013 this sequence version replaced XM_005173266.1, XM_001336587.1. ##Evidence-Data-START## Transcript exon combination :: BC162382.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTACCCAA GGGTCATCCT AAAGGAGACG CTGTACAAGA AGTCACAGCA GAAGAGAAGA 
ACATCACCAT GCAATTACAA AGAGAGGACA TTCATATTAA ACACACAGGA TCTGGCTTAC 
TTTGAACAGC GTCCCGGGAA AAAGCCAACT CAAAAAGGCT GCATCGAGCT TTCTCATATA 
AAGTGTGTGG AGATTGTGCG CAGTGATGTT CCCATTCCCT GTGATTACAA GTATCCTTTC 
CAGATCATCC ATGACAGCTA CTATCTGTAT GTGTTTGCTC CAGACAACGA CTGCAGGTTA 
AAATGGGTCA AAGCTTTAAA GGAAGCCATA CAAAATAACA GCTTATTCCT GAAATACCAT 
CCTGACTTCT GGGGAGAAGG AAAATGGAGA TGTTGTCAGC AGACGGAGAA GCTGGCGACG 
GGATGCAGTG AATACTATCC CAATGGAGCC AAACCTCTTC CGCCAACGCC GGATCCATCG 
CGCCGATCCT CAGAGCCAGA GGAGAGGATT GTTGTGGCTT TGAGGAATTT CATCCCTAAA 
GAACACACAG ATCTGCCTCT GCAAAAAGAT GAAGAGTATT TTATTGTTGA TTGTTCCGAA 
CCCAACTGGT GGACCGTGAA AGACAAAGAC GGAAATGTTG GTTCTGTGCC ATGCATATAC 
GTTACAGAAA AACTCTCAAA CAACATTGAG AAATTTGAAT GGTACAATAA GGACATAAAT 
CGAACGGAGG CAGAGGACTT ACTAATGCAA GAGAACAAGG ACGGGGGTTT CATGGTTCGT 
AATTCTAAGC ATGCAGGTGT TTACACCGTG TCCATATTCA CCAGAGTTTC AGGGTCTAAT 
GGCGAGAAAT GTCCCCAAGT TAAGCACTAC CAGATCAAAG TACAGAGTGA AGGTGAAAAA 
GTCTTGTATT ACTTAGCTGA GAAATATTTG TTCCCAACCA TTCCAGAGCT CATCCGCTAC 
CACCAACACA ATCCGGCAGG TTTGATCACC AGACTGAGGC ACTGTGTGTC TAGAAATGTC 
CCAAACTCTG CAAGGATCAA GGAGTTATCA TCAGATAAGT GGGAGATTCA CCCTGATGAA 
GTGAAATTAA GTGTGGAACT GGGCAGTGGG CAGTTTGGCT TGGTGTGGAA GGGAAGTTGG 
CAAGACAGGG AGGTGGCAGT TAAGACGGTT CGTGAGGGAT TTATGTCAGA GGAGGAATTC 
AAAGAAGAAG CACAAGTTAT GATGAAACTA TCCCACAATA GGCTGGTGCA GTTGTATGGT 
GTGGTCACTC AGCGTTCACC CATATACCTG GTATTCGAAT TCATGGAAAA CGGTTGCCTT 
ACGGAGTTCT TGCGGGCCAA AAAAGGCAAA CTTTCCCAAA AGGCTCTGCT GGAGATGTGT 
TTGGACGTAA GTGAAGGAAT GGCCTACCTT GAAAGCTCTA ACTTCATCCA CAGGGACTTG 
GCTGCGAGAA ACTGTTTGGT TTCTGAAAAT AGTGTGGTGA AGATCGCAGA TTTTGGAATG 
ACAAGGTTTG TCCTGGATGA CCAGTATACA AGTTCTCATG GATCCAAATT TCCAGTCAGG 
TGGTCTTCTC CAGAGGTGAT CAAATTTGGG AAGTACAGCA GCAAATCAGA TGTGTGGTCA 
TTTGGTGTGT TCATGTGGGA GGTGTACAGT GAGGGACGCA CACCCTATGA CAACCGTAGC 
AATTCAGAAG TAGTGGAGAC CCTAAATGCA GGGCATAGAC TCCTGAAACC ACGGCTCTGT 
CCTCAGAGTG TGTACGAGTT AATGCAGTGG AGCTGGAAAG AGAAACCTGA GGATCGACCC 
TCATTTGCGC TTCTCCTGCA TGAACTGGCC TGCCTTGATC AAGAACCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571179.1
CDS	80..1930
Translation	MYPRVILKETLYKKSQQKRRTSPCNYKERTFILNTQDLAYFEQRPGKKPTQKGCIELSHIKCVEIVRSDVPIPCDYKYPFQIIHDSYYLYVFAPDNDCRLKWVKALKEAIQNNSLFLKYHPDFWGEGKWRCCQQTEKLATGCSEYYPNGAKPLPPTPDPSRRSSEPEERIVVALRNFIPKEHTDLPLQKDEEYFIVDCSEPNWWTVKDKDGNVGSVPCIYVTEKLSNNIEKFEWYNKDINRTEAEDLLMQENKDGGFMVRNSKHAGVYTVSIFTRVSGSNGEKCPQVKHYQIKVQSEGEKVLYYLAEKYLFPTIPELIRYHQHNPAGLITRLRHCVSRNVPNSARIKELSSDKWEIHPDEVKLSVELGSGQFGLVWKGSWQDREVAVKTVREGFMSEEEFKEEAQVMMKLSHNRLVQLYGVVTQRSPIYLVFEFMENGCLTEFLRAKKGKLSQKALLEMCLDVSEGMAYLESSNFIHRDLAARNCLVSENSVVKIADFGMTRFVLDDQYTSSHGSKFPVRWSSPEVIKFGKYSSKSDVWSFGVFMWEVYSEGRTPYDNRSNSEVVETLNAGHRLLKPRLCPQSVYELMQWSWKEKPEDRPSFALLLHELACLDQEP

Target ORF information:

RefSeq Version	NM_131104.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio IL2 inducible T cell kinase (itk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131104.1

ORF Insert Sequence:

ATGTACCCAA GGGTCATCCT AAAGGAGACG CTGTACAAGA AGTCACAGCA GAAGAGAAGA 
ACATCACCAT GCAATTACAA AGAGAGGACA TTCATATTAA ACACACAGGA TCTGGCTTAC 
TTTGAACAGC GTCCCGGGAA AAAGCCAACT CAAAAAGGCT GCATCGAGCT TTCTCATATA 
AAGTGTGTGG AGATTGTGCG CAGTGATGTT CCCATTCCCT GTGATTACAA GTATCCTTTC 
CAGATCATCC ATGACAGCTA CTATCTGTAT GTGTTTGCTC CAGACAACGA CTGCAGGTTA 
AAATGGGTCA AAGCTTTAAA GGAAGCCATA CAAAATAACA GCTTATTCCT GAAATACCAT 
CCTGACTTCT GGGGAGAAGG AAAATGGAGA TGTTGTCAGC AGACGGAGAA GCTGGCGACG 
GGATGCAGTG AATACTATCC CAATGGAGCC AAACCTCTTC CGCCAACGCC GGATCCATCG 
CGCCGATCCT CAGAGCCAGA GGAGAGGATT GTTGTGGCTT TGAGGAATTT CATCCCTAAA 
GAACACACAG ATCTGCCTCT GCAAAAAGAT GAAGAGTATT TTATTGTTGA TTGTTCCGAA 
CCCAACTGGT GGACCGTGAA AGACAAAGAC GGAAATGTTG GTTCTGTGCC ATGCATATAC 
GTTACAGAAA AACTCTCAAA CAACATTGAG AAATTTGAAT GGTACAATAA GGACATAAAT 
CGAACGGAGG CAGAGGACTT ACTAATGCAA GAGAACAAGG ACGGGGGTTT CATGGTTCGT 
AATTCTAAGC ATGCAGGTGT TTACACCGTG TCCATATTCA CCAGAGTTTC AGGGTCTAAT 
GGCGAGAAAT GTCCCCAAGT TAAGCACTAC CAGATCAAAG TACAGAGTGA AGGTGAAAAA 
GTCTTGTATT ACTTAGCTGA GAAATATTTG TTCCCAACCA TTCCAGAGCT CATCCGCTAC 
CACCAACACA ATCCGGCAGG TTTGATCACC AGACTGAGGC ACTGTGTGTC TAGAAATGTC 
CCAAACTCTG CAAGGATCAA GGAGTTATCA TCAGATAAGT GGGAGATTCA CCCTGATGAA 
GTGAAATTAA GTGTGGAACT GGGCAGTGGG CAGTTTGGCT TGGTGTGGAA GGGAAGTTGG 
CAAGACAGGG AGGTGGCAGT TAAGACGGTT CGTGAGGGAT TTATGTCAGA GGAGGAATTC 
AAAGAAGAAG CACAAGTTAT GATGAAACTA TCCCACAATA GGCTGGTGCA GTTGTATGGT 
GTGGTCACTC AGCGTTCACC CATATACCTG GTATTCGAAT TCATGGAAAA CGGTTGCCTT 
ACGGAGTTCT TGCGGGCCAA AAAAGGCAAA CTTTCCCAAA AGGCTCTGCT GGAGATGTGT 
TTGGACGTAA GTGAAGGAAT GGCCTACCTT GAAAGCTCTA ACTTCATCCA CAGGGACTTG 
GCTGCGAGAA ACTGTTTGGT TTCTGAAAAT AGTGTGGTGA AGATCGCAGA TTTTGGAATG 
ACAAGGTTTG TCCTGGATGA CCAGTATACA AGTTCTCATG GATCCAAATT TCCAGTCAGG 
TGGTCTTCTC CAGAGGTGAT CAAATTTGGG AAGTACAGCA GCAAATCAGA TGTGTGGTCA 
TTTGGTGTGT TCATGTGGGA GGTGTACAGT GAGGGACGCA CACCCTATGA CAACCGTAGC 
AATTCAGAAG TAGTGGAGAC CCTAAATGCA GGGCATAGAC TCCTGAAACC ACGGCTCTGT 
CCTCAGAGTG TGTACGAGTT AATGCAGTGG AGCTGGAAAG AGAAACCTGA GGATCGACCC 
TCATTTGCGC TTCTCCTGCA TGAACTGGCC TGCCTTGATC AAGAACCTTG A