cux1a gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_005172720.4	XP_005172777.1	homeobox protein cut-like 1 isoform X3
XM_005172718.3	XP_005172775.1	homeobox protein cut-like 1 isoform X1
XM_005172719.4	XP_005172776.1	homeobox protein cut-like 1 isoform X2
XM_021479403.1	XP_021335078.1	homeobox protein cut-like 1 isoform X6
XM_005172720.3	XP_005172777.1	homeobox protein cut-like 1 isoform X3
XM_003199366.4	XP_003199414.2	homeobox protein cut-like 1 isoform X5
XM_005172718.4	XP_005172775.1	homeobox protein cut-like 1 isoform X1
XM_005172721.4	XP_005172778.1	homeobox protein cut-like 1 isoform X4
XM_021479404.1	XP_021335079.1	homeobox protein cut-like 1 isoform X7
XM_005172719.3	XP_005172776.1	homeobox protein cut-like 1 isoform X2
XM_005172721.3	XP_005172778.1	homeobox protein cut-like 1 isoform X4
XM_003199366.5	XP_003199414.2	homeobox protein cut-like 1 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa147732	XM_021479403.1 Latest version!	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa147733	XM_021479404.1 Latest version!	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa16296	XM_005172718.4 Latest version!	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa16297	XM_005172719.4 Latest version!	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa16298	XM_005172720.4 Latest version!	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa16299	XM_005172721.4 Latest version!	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa16300	XM_003199366.5 Latest version!	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa16298	XM_005172720.3	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa16296	XM_005172718.3	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa16297	XM_005172719.3	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa16299	XM_005172721.3	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa16300	XM_003199366.4	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

cux1a ( XM_021479403.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335078.1 Danio rerio cut-like homeobox 1a (cux1a), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa147732
Clone ID Related Accession (Same CDS sequence)	XM_021479403.1
Accession Version	XM_021479403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	homeobox protein cut-like 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGGTAACAT CACCACACGG ATCCGCACGC CTGAAACCGG CTCCGATGAA 
GCCATCAAGT CCATCTTAGA GCAGGCCAAG AGAGAGTTAC AAGTGCAGAA AGCAGAAGAA 
TCTAACATAG ATCTGAAACC CATGAGGATG ACTAAGCAAT TCGAGAGCTC TCAGCCTCCG 
TCCTCCTCCA GCAACTCGGA CGAGAACATT CGCTCCATCC TCGAGCAGGC TCGCAGGGAG 
ATGGAGGCGC AGCAGGTGGC ACTGGAGCCC GTCTTGAAGG CCAGCTCCAT GGGCCCGACG 
GACCTCTCGC TCCTGGGCTC CAAACTCCTG GGCTCCCCGC TCTCCACCCT GCCCTACACC 
CCCATCACCC AGTCCCTGAA GAAGCCCTCT AGTTCACCGC TGCTGGACTT CCACCATGGT 
GGAGTGAAGA AGGAGATGGG CGAGGTGGCC GTTTCGGATG TGGGTCTGGA CGGAGTGGTG 
CCCAAGCTGG GCCGTTTGTC TGAGAGCGGT TTGGGTGGTG GCTCGTGGAG GGAGCACTGG 
TGGAGCACAG GCCTGTCAGA GCGCCGCGGG TCCAGCCAGC CCTCCGTTAG TGAAGACGCA 
CACAGTCAAG AGGACAGCAA AGAGAAGCTC CCTCGGCTGG GCAGCACTGC GCCGGTGGCT 
CCTGCGTCGT CTCTGGATTA CTGGAAGGAC TGGCCGAGCT CAGAATCGCC GTACTCTCAG 
AGCTCAGAGC TCAGCCTGCA GATGCCCAGT GGCAGTGAGA CCCCTCAGAG CAGCCCACTG 
CCTTCCTCCT CGCCCTTACT GTCCCTGCCC AAAGTGGCCA AACCTGTGGT GCCGCCGCTT 
ACGCCAGAGC AATACGAGTA CTACATGTAC CAGGAGGTGG ACACTGTCGA CCTCACACAG 
CAGGTCAAAG AAAAACTCGC CAAGAACGGC ATCTGCCAGC GCATCTTCGG GGAGAAGGTG 
CTGGGTCTCT CTCAGGGGAG TGTGAGCGAC ATGCTCTCTC GACCAAAGCA CTGGAGCAAA 
CTCACACAGA AGGGAAGGGA GCCGTTTATA CGCATGCAGT TATGGCTGAA CGGAGAGCTG 
GGACAGGCAC TGATGCCAAT GCCTGGACAG ACACAAGAAA TCACCCCCAA AACCTCGGCC 
AGCTGCAGTC CTGCCCCAGA ATCCCCCCTG AGCTCGGCTG AGGAGTCTGT GAAGAGCCAG 
CAGGAGGAGC AGATGTGCCG ATCCTCCGTC CAGGAGCCCA GCGAAGCCTC CGAGTCTCAG 
CCCGGGACGC CGCTGCCGCT GCCTCTGCCG GGACAGGCCG GTCTCAGCAT TCAGGAGATG 
GTGGCCATGT CAGCCGAGCT GGACACGTAC GTCATCACGA AAAAAGTCAA AGAAGTCCTC 
ACTGATAACA ACCTGGGCCA GCGTCTGTTC GGGGAGAGCA TTCTGGGTTT GACGCAGGGC 
TCAGTGTCTG ATCTGCTGGC TCGACCCAAA CCCTGGCACA AGCTCAGCCT GAAGGGTCGC 
GAGCCGTTCG TGCGCATGCA GCTGTGGTTG AGCGACCCGC GCAACGTGGA GAAGCTGATG 
GACATGAAGC GCATGGAGAA GAAAGCCTAC ATGAAAAGGC GCCACAGCTC CATCGGTGAG 
AGTCATTCGC TGGAGAGCGG CTTGTCTGCA GCAGACCTGA CACAGGGTGC CAGTCCCCAA 
CACCTATCCC AGCAGCAGCC GCCGCAGCAG ATGCAGCCTC AACAGCAGCA GCCCCAGCTG 
AAGAAACCCC GGGTGGTTCT GGCTCCGGAA GAGAAGGAAG CTCTGAAGCG GGCCTACCAG 
CAGAAGCCTT ACCCCTCCCC TAAAACCATT GAGGAGTTAG CGAGTCAGCT CAACCTCAAA 
ACCAGCACCG TCATCAACTG GTTCCACAAC TACAGGTCTC GTATCCGGCG AGAGCTTTTT 
ATCGAAGAGA TCCAGGCAGC AGCAGGTGAA GGTGGATCTC CATCTGCACG GCCTGGAAAA 
TCTGGAGGCG AGGGAGAAAA CAGCTGCGAC GGCACTGAGT CCGACAGCAC GGCAGAAGGC 
CGACCGAGTG GCTCAGAGGA GCGCAAGGGC TTCCTGGAGA GCCCGGGCCT CGGCCACAGA 
GAAGAAGATG GCGAGGGCGA GTTTCCTAGT GCCTCGGGCA CCAGCCGGCC ACGAGGAGGC 
ATCATAGACA GCCTGTTCGG CATCCCCGAG TCCATATCCG TCACCAATGC TGCCACGACC 
CCCTGCCGAA ATCCAAAGGA TAGCAGTCTA CGCAAGAAGA AAGCAGCCAA CCTCAACAAC 
ATCATCCACC GGCTGGAGAA AGCGGCGAAC CGAGACGAGC CTCACGAATG GGAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335078.1
CDS	79..4398
Translation	MAANAGSMFQYWKRFDLQQLQKELDATATALANRQDESEQSRKKLIDQSREFKKNTPEDLRKQIAPLLKGFQAEIDALSKRSKESEAAFLSVYKRLIDVPDPVSALEAAQQLQVTVRKMHDLETENQRLKDTLQEYEREISEVKNQEVTIKALKEKLEQYERSLQAKNTEEAVEPNLDSSEREERQSKGENLPNNYADKESQPEQSQESRTEDSDVKTQSLQTALEATQAELHELKTKYDEESTAKANEIEAVMTDLERANQRAETAEREAGTLREQLTSAGKTMQSSNQIQNAPEMDKSLEAELGAKEREVERLAEDVQRLQASLAQLSETSTNQISQLEQQLSSKEALLKQLEEKLQDQSDYEEIKRELCTLKSVEHNSSDHHSSAQDSSKPLEMLLTGKDHSQLSESALKRLANSDFTGAVGRKLKETTVENPQRLPATPVPTTLQGSPPPPSPQLLMRTGTATSESAASANGTHPFSPTGIGPDFFTPGMASVGATFPPLAGKMALNSLLQRQLMQTIYTKALQDPSPSSVLFGPPHYATTNSLSSPLPPQSAGSPEVNGMAPSPSQSEGAGSTSEGEEMDTAEIARQVKEQLIKHNIGQRVFGHYVLGLSQGSVSEILARPKPWSKLTIRGKEPFHKMKHFLSDEQNILALRSIQGRQRGNITTRIRTPETGSDEAIKSILEQAKRELQVQKAEESNIDLKPMRMTKQFESSQPPSSSSNSDENIRSILEQARREMEAQQVALEPVLKASSMGPTDLSLLGSKLLGSPLSTLPYTPITQSLKKPSSSPLLDFHHGGVKKEMGEVAVSDVGLDGVVPKLGRLSESGLGGGSWREHWWSTGLSERRGSSQPSVSEDAHSQEDSKEKLPRLGSTAPVAPASSLDYWKDWPSSESPYSQSSELSLQMPSGSETPQSSPLPSSSPLLSLPKVAKPVVPPLTPEQYEYYMYQEVDTVDLTQQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKHWSKLTQKGREPFIRMQLWLNGELGQALMPMPGQTQEITPKTSASCSPAPESPLSSAEESVKSQQEEQMCRSSVQEPSEASESQPGTPLPLPLPGQAGLSIQEMVAMSAELDTYVITKKVKEVLTDNNLGQRLFGESILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLSDPRNVEKLMDMKRMEKKAYMKRRHSSIGESHSLESGLSAADLTQGASPQHLSQQQPPQQMQPQQQQPQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEELASQLNLKTSTVINWFHNYRSRIRRELFIEEIQAAAGEGGSPSARPGKSGGEGENSCDGTESDSTAEGRPSGSEERKGFLESPGLGHREEDGEGEFPSASGTSRPRGGIIDSLFGIPESISVTNAATTPCRNPKDSSLRKKKAANLNNIIHRLEKAANRDEPHEWEF

Target ORF information:

RefSeq Version	XM_021479403.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479403.1

ORF Insert Sequence:

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGGTAACAT CACCACACGG ATCCGCACGC CTGAAACCGG CTCCGATGAA 
GCCATCAAGT CCATCTTAGA GCAGGCCAAG AGAGAGTTAC AAGTGCAGAA AGCAGAAGAA 
TCTAACATAG ATCTGAAACC CATGAGGATG ACTAAGCAAT TCGAGAGCTC TCAGCCTCCG 
TCCTCCTCCA GCAACTCGGA CGAGAACATT CGCTCCATCC TCGAGCAGGC TCGCAGGGAG 
ATGGAGGCGC AGCAGGTGGC ACTGGAGCCC GTCTTGAAGG CCAGCTCCAT GGGCCCGACG 
GACCTCTCGC TCCTGGGCTC CAAACTCCTG GGCTCCCCGC TCTCCACCCT GCCCTACACC 
CCCATCACCC AGTCCCTGAA GAAGCCCTCT AGTTCACCGC TGCTGGACTT CCACCATGGT 
GGAGTGAAGA AGGAGATGGG CGAGGTGGCC GTTTCGGATG TGGGTCTGGA CGGAGTGGTG 
CCCAAGCTGG GCCGTTTGTC TGAGAGCGGT TTGGGTGGTG GCTCGTGGAG GGAGCACTGG 
TGGAGCACAG GCCTGTCAGA GCGCCGCGGG TCCAGCCAGC CCTCCGTTAG TGAAGACGCA 
CACAGTCAAG AGGACAGCAA AGAGAAGCTC CCTCGGCTGG GCAGCACTGC GCCGGTGGCT 
CCTGCGTCGT CTCTGGATTA CTGGAAGGAC TGGCCGAGCT CAGAATCGCC GTACTCTCAG 
AGCTCAGAGC TCAGCCTGCA GATGCCCAGT GGCAGTGAGA CCCCTCAGAG CAGCCCACTG 
CCTTCCTCCT CGCCCTTACT GTCCCTGCCC AAAGTGGCCA AACCTGTGGT GCCGCCGCTT 
ACGCCAGAGC AATACGAGTA CTACATGTAC CAGGAGGTGG ACACTGTCGA CCTCACACAG 
CAGGTCAAAG AAAAACTCGC CAAGAACGGC ATCTGCCAGC GCATCTTCGG GGAGAAGGTG 
CTGGGTCTCT CTCAGGGGAG TGTGAGCGAC ATGCTCTCTC GACCAAAGCA CTGGAGCAAA 
CTCACACAGA AGGGAAGGGA GCCGTTTATA CGCATGCAGT TATGGCTGAA CGGAGAGCTG 
GGACAGGCAC TGATGCCAAT GCCTGGACAG ACACAAGAAA TCACCCCCAA AACCTCGGCC 
AGCTGCAGTC CTGCCCCAGA ATCCCCCCTG AGCTCGGCTG AGGAGTCTGT GAAGAGCCAG 
CAGGAGGAGC AGATGTGCCG ATCCTCCGTC CAGGAGCCCA GCGAAGCCTC CGAGTCTCAG 
CCCGGGACGC CGCTGCCGCT GCCTCTGCCG GGACAGGCCG GTCTCAGCAT TCAGGAGATG 
GTGGCCATGT CAGCCGAGCT GGACACGTAC GTCATCACGA AAAAAGTCAA AGAAGTCCTC 
ACTGATAACA ACCTGGGCCA GCGTCTGTTC GGGGAGAGCA TTCTGGGTTT GACGCAGGGC 
TCAGTGTCTG ATCTGCTGGC TCGACCCAAA CCCTGGCACA AGCTCAGCCT GAAGGGTCGC 
GAGCCGTTCG TGCGCATGCA GCTGTGGTTG AGCGACCCGC GCAACGTGGA GAAGCTGATG 
GACATGAAGC GCATGGAGAA GAAAGCCTAC ATGAAAAGGC GCCACAGCTC CATCGGTGAG 
AGTCATTCGC TGGAGAGCGG CTTGTCTGCA GCAGACCTGA CACAGGGTGC CAGTCCCCAA 
CACCTATCCC AGCAGCAGCC GCCGCAGCAG ATGCAGCCTC AACAGCAGCA GCCCCAGCTG 
AAGAAACCCC GGGTGGTTCT GGCTCCGGAA GAGAAGGAAG CTCTGAAGCG GGCCTACCAG 
CAGAAGCCTT ACCCCTCCCC TAAAACCATT GAGGAGTTAG CGAGTCAGCT CAACCTCAAA 
ACCAGCACCG TCATCAACTG GTTCCACAAC TACAGGTCTC GTATCCGGCG AGAGCTTTTT 
ATCGAAGAGA TCCAGGCAGC AGCAGGTGAA GGTGGATCTC CATCTGCACG GCCTGGAAAA 
TCTGGAGGCG AGGGAGAAAA CAGCTGCGAC GGCACTGAGT CCGACAGCAC GGCAGAAGGC 
CGACCGAGTG GCTCAGAGGA GCGCAAGGGC TTCCTGGAGA GCCCGGGCCT CGGCCACAGA 
GAAGAAGATG GCGAGGGCGA GTTTCCTAGT GCCTCGGGCA CCAGCCGGCC ACGAGGAGGC 
ATCATAGACA GCCTGTTCGG CATCCCCGAG TCCATATCCG TCACCAATGC TGCCACGACC 
CCCTGCCGAA ATCCAAAGGA TAGCAGTCTA CGCAAGAAGA AAGCAGCCAA CCTCAACAAC 
ATCATCCACC GGCTGGAGAA AGCGGCGAAC CGAGACGAGC CTCACGAATG GGAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cux1a ( XM_021479404.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335079.1 Danio rerio cut-like homeobox 1a (cux1a), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa147733
Clone ID Related Accession (Same CDS sequence)	XM_021479404.1
Accession Version	XM_021479404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	homeobox protein cut-like 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGGTAACAT CACCACACGG ATCCGCACGC CTGAAACCGG CTCCGATGAA 
GCCATCAAGT CCATCTTAGA GCAGGCCAAG AGAGAGTTAC AAGTGCAGAA AGCAGTCGAG 
AGCTCTCAGC CTCCGTCCTC CTCCAGCAAC TCGGACGAGA ACATTCGCTC CATCCTCGAG 
CAGGCTCGCA GGGAGATGGA GGCGCAGCAG GTGGCACTGG AGCCCGTCTT GAAGGCCAGC 
TCCATGGGCC CGACGGACCT CTCGCTCCTG GGCTCCAAAC TCCTGGGCTC CCCGCTCTCC 
ACCCTGCCCT ACACCCCCAT CACCCAGTCC CTGAAGAAGC CCTCTAGTTC ACCGCTGCTG 
GACTTCCACC ATGGTGGAGT GAAGAAGGAG ATGGGCGAGG TGGCCGTTTC GGATGTGGGT 
CTGGACGGAG TGGTGCCCAA GCTGGGCCGT TTGTCTGAGA GCGGTTTGGG TGGTGGCTCG 
TGGAGGGAGC ACTGGTGGAG CACAGGCCTG TCAGAGCGCC GCGGGTCCAG CCAGCCCTCC 
GTTAGTGAAG ACGCACACAG TCAAGAGGAC AGCAAAGAGA AGCTCCCTCG GCTGGGCAGC 
ACTGCGCCGG TGGCTCCTGC GTCGTCTCTG GATTACTGGA AGGACTGGCC GAGCTCAGAA 
TCGCCGTACT CTCAGAGCTC AGAGCTCAGC CTGCAGATGC CCAGTGGCAG TGAGACCCCT 
CAGAGCAGCC CACTGCCTTC CTCCTCGCCC TTACTGTCCC TGCCCAAAGT GGCCAAACCT 
GTGGTGCCGC CGCTTACGCC AGAGCAATAC GAGTACTACA TGTACCAGGA GGTGGACACT 
GTCGACCTCA CACAGCAGGT CAAAGAAAAA CTCGCCAAGA ACGGCATCTG CCAGCGCATC 
TTCGGGGAGA AGGTGCTGGG TCTCTCTCAG GGGAGTGTGA GCGACATGCT CTCTCGACCA 
AAGCACTGGA GCAAACTCAC ACAGAAGGGA AGGGAGCCGT TTATACGCAT GCAGTTATGG 
CTGAACGGAG AGCTGGGACA GGCACTGATG CCAATGCCTG GACAGACACA AGAAATCACC 
CCCAAAACCT CGGCCAGCTG CAGTCCTGCC CCAGAATCCC CCCTGAGCTC GGCTGAGGAG 
TCTGTGAAGA GCCAGCAGGA GGAGCAGATG TGCCGATCCT CCGTCCAGGA GCCCAGCGAA 
GCCTCCGAGT CTCAGCCCGG GACGCCGCTG CCGCTGCCTC TGCCGGGACA GGCCGGTCTC 
AGCATTCAGG AGATGGTGGC CATGTCAGCC GAGCTGGACA CGTACGTCAT CACGAAAAAA 
GTCAAAGAAG TCCTCACTGA TAACAACCTG GGCCAGCGTC TGTTCGGGGA GAGCATTCTG 
GGTTTGACGC AGGGCTCAGT GTCTGATCTG CTGGCTCGAC CCAAACCCTG GCACAAGCTC 
AGCCTGAAGG GTCGCGAGCC GTTCGTGCGC ATGCAGCTGT GGTTGAGCGA CCCGCGCAAC 
GTGGAGAAGC TGATGGACAT GAAGCGCATG GAGAAGAAAG CCTACATGAA AAGGCGCCAC 
AGCTCCATCG GTGAGAGTCA TTCGCTGGAG AGCGGCTTGT CTGCAGCAGA CCTGACACAG 
GGTGCCAGTC CCCAACACCT ATCCCAGCAG CAGCCGCCGC AGCAGATGCA GCCTCAACAG 
CAGCAGCCCC AGCTGAAGAA ACCCCGGGTG GTTCTGGCTC CGGAAGAGAA GGAAGCTCTG 
AAGCGGGCCT ACCAGCAGAA GCCTTACCCC TCCCCTAAAA CCATTGAGGA GTTAGCGAGT 
CAGCTCAACC TCAAAACCAG CACCGTCATC AACTGGTTCC ACAACTACAG GTCTCGTATC 
CGGCGAGAGC TTTTTATCGA AGAGATCCAG GCAGCAGCAG GTGAAGGTGG ATCTCCATCT 
GCACGGCCTG GAAAATCTGG AGGCGAGGGA GAAAACAGCT GCGACGGCAC TGAGTCCGAC 
AGCACGGCAG AAGGCCGACC GAGTGGCTCA GAGGAGCGCA AGGGCTTCCT GGAGAGCCCG 
GGCCTCGGCC ACAGAGAAGA AGATGGCGAG GGCGAGTTTC CTAGTGCCTC GGGCACCAGC 
CGGCCACGAG GAGGCATCAT AGACAGCCTG TTCGGCATCC CCGAGTCCAT ATCCGTCACC 
AATGCTGCCA CGACCCCCTG CCGAAATCCA AAGGATAGCA GTCTACGCAA GAAGAAAGCA 
GCCAACCTCA ACAACATCAT CCACCGGCTG GAGAAAGCGG CGAACCGAGA CGAGCCTCAC 
GAATGGGAGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335079.1
CDS	79..4353
Translation	MAANAGSMFQYWKRFDLQQLQKELDATATALANRQDESEQSRKKLIDQSREFKKNTPEDLRKQIAPLLKGFQAEIDALSKRSKESEAAFLSVYKRLIDVPDPVSALEAAQQLQVTVRKMHDLETENQRLKDTLQEYEREISEVKNQEVTIKALKEKLEQYERSLQAKNTEEAVEPNLDSSEREERQSKGENLPNNYADKESQPEQSQESRTEDSDVKTQSLQTALEATQAELHELKTKYDEESTAKANEIEAVMTDLERANQRAETAEREAGTLREQLTSAGKTMQSSNQIQNAPEMDKSLEAELGAKEREVERLAEDVQRLQASLAQLSETSTNQISQLEQQLSSKEALLKQLEEKLQDQSDYEEIKRELCTLKSVEHNSSDHHSSAQDSSKPLEMLLTGKDHSQLSESALKRLANSDFTGAVGRKLKETTVENPQRLPATPVPTTLQGSPPPPSPQLLMRTGTATSESAASANGTHPFSPTGIGPDFFTPGMASVGATFPPLAGKMALNSLLQRQLMQTIYTKALQDPSPSSVLFGPPHYATTNSLSSPLPPQSAGSPEVNGMAPSPSQSEGAGSTSEGEEMDTAEIARQVKEQLIKHNIGQRVFGHYVLGLSQGSVSEILARPKPWSKLTIRGKEPFHKMKHFLSDEQNILALRSIQGRQRGNITTRIRTPETGSDEAIKSILEQAKRELQVQKAVESSQPPSSSSNSDENIRSILEQARREMEAQQVALEPVLKASSMGPTDLSLLGSKLLGSPLSTLPYTPITQSLKKPSSSPLLDFHHGGVKKEMGEVAVSDVGLDGVVPKLGRLSESGLGGGSWREHWWSTGLSERRGSSQPSVSEDAHSQEDSKEKLPRLGSTAPVAPASSLDYWKDWPSSESPYSQSSELSLQMPSGSETPQSSPLPSSSPLLSLPKVAKPVVPPLTPEQYEYYMYQEVDTVDLTQQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKHWSKLTQKGREPFIRMQLWLNGELGQALMPMPGQTQEITPKTSASCSPAPESPLSSAEESVKSQQEEQMCRSSVQEPSEASESQPGTPLPLPLPGQAGLSIQEMVAMSAELDTYVITKKVKEVLTDNNLGQRLFGESILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLSDPRNVEKLMDMKRMEKKAYMKRRHSSIGESHSLESGLSAADLTQGASPQHLSQQQPPQQMQPQQQQPQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEELASQLNLKTSTVINWFHNYRSRIRRELFIEEIQAAAGEGGSPSARPGKSGGEGENSCDGTESDSTAEGRPSGSEERKGFLESPGLGHREEDGEGEFPSASGTSRPRGGIIDSLFGIPESISVTNAATTPCRNPKDSSLRKKKAANLNNIIHRLEKAANRDEPHEWEF

Target ORF information:

RefSeq Version	XM_021479404.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479404.1

ORF Insert Sequence:

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGGTAACAT CACCACACGG ATCCGCACGC CTGAAACCGG CTCCGATGAA 
GCCATCAAGT CCATCTTAGA GCAGGCCAAG AGAGAGTTAC AAGTGCAGAA AGCAGTCGAG 
AGCTCTCAGC CTCCGTCCTC CTCCAGCAAC TCGGACGAGA ACATTCGCTC CATCCTCGAG 
CAGGCTCGCA GGGAGATGGA GGCGCAGCAG GTGGCACTGG AGCCCGTCTT GAAGGCCAGC 
TCCATGGGCC CGACGGACCT CTCGCTCCTG GGCTCCAAAC TCCTGGGCTC CCCGCTCTCC 
ACCCTGCCCT ACACCCCCAT CACCCAGTCC CTGAAGAAGC CCTCTAGTTC ACCGCTGCTG 
GACTTCCACC ATGGTGGAGT GAAGAAGGAG ATGGGCGAGG TGGCCGTTTC GGATGTGGGT 
CTGGACGGAG TGGTGCCCAA GCTGGGCCGT TTGTCTGAGA GCGGTTTGGG TGGTGGCTCG 
TGGAGGGAGC ACTGGTGGAG CACAGGCCTG TCAGAGCGCC GCGGGTCCAG CCAGCCCTCC 
GTTAGTGAAG ACGCACACAG TCAAGAGGAC AGCAAAGAGA AGCTCCCTCG GCTGGGCAGC 
ACTGCGCCGG TGGCTCCTGC GTCGTCTCTG GATTACTGGA AGGACTGGCC GAGCTCAGAA 
TCGCCGTACT CTCAGAGCTC AGAGCTCAGC CTGCAGATGC CCAGTGGCAG TGAGACCCCT 
CAGAGCAGCC CACTGCCTTC CTCCTCGCCC TTACTGTCCC TGCCCAAAGT GGCCAAACCT 
GTGGTGCCGC CGCTTACGCC AGAGCAATAC GAGTACTACA TGTACCAGGA GGTGGACACT 
GTCGACCTCA CACAGCAGGT CAAAGAAAAA CTCGCCAAGA ACGGCATCTG CCAGCGCATC 
TTCGGGGAGA AGGTGCTGGG TCTCTCTCAG GGGAGTGTGA GCGACATGCT CTCTCGACCA 
AAGCACTGGA GCAAACTCAC ACAGAAGGGA AGGGAGCCGT TTATACGCAT GCAGTTATGG 
CTGAACGGAG AGCTGGGACA GGCACTGATG CCAATGCCTG GACAGACACA AGAAATCACC 
CCCAAAACCT CGGCCAGCTG CAGTCCTGCC CCAGAATCCC CCCTGAGCTC GGCTGAGGAG 
TCTGTGAAGA GCCAGCAGGA GGAGCAGATG TGCCGATCCT CCGTCCAGGA GCCCAGCGAA 
GCCTCCGAGT CTCAGCCCGG GACGCCGCTG CCGCTGCCTC TGCCGGGACA GGCCGGTCTC 
AGCATTCAGG AGATGGTGGC CATGTCAGCC GAGCTGGACA CGTACGTCAT CACGAAAAAA 
GTCAAAGAAG TCCTCACTGA TAACAACCTG GGCCAGCGTC TGTTCGGGGA GAGCATTCTG 
GGTTTGACGC AGGGCTCAGT GTCTGATCTG CTGGCTCGAC CCAAACCCTG GCACAAGCTC 
AGCCTGAAGG GTCGCGAGCC GTTCGTGCGC ATGCAGCTGT GGTTGAGCGA CCCGCGCAAC 
GTGGAGAAGC TGATGGACAT GAAGCGCATG GAGAAGAAAG CCTACATGAA AAGGCGCCAC 
AGCTCCATCG GTGAGAGTCA TTCGCTGGAG AGCGGCTTGT CTGCAGCAGA CCTGACACAG 
GGTGCCAGTC CCCAACACCT ATCCCAGCAG CAGCCGCCGC AGCAGATGCA GCCTCAACAG 
CAGCAGCCCC AGCTGAAGAA ACCCCGGGTG GTTCTGGCTC CGGAAGAGAA GGAAGCTCTG 
AAGCGGGCCT ACCAGCAGAA GCCTTACCCC TCCCCTAAAA CCATTGAGGA GTTAGCGAGT 
CAGCTCAACC TCAAAACCAG CACCGTCATC AACTGGTTCC ACAACTACAG GTCTCGTATC 
CGGCGAGAGC TTTTTATCGA AGAGATCCAG GCAGCAGCAG GTGAAGGTGG ATCTCCATCT 
GCACGGCCTG GAAAATCTGG AGGCGAGGGA GAAAACAGCT GCGACGGCAC TGAGTCCGAC 
AGCACGGCAG AAGGCCGACC GAGTGGCTCA GAGGAGCGCA AGGGCTTCCT GGAGAGCCCG 
GGCCTCGGCC ACAGAGAAGA AGATGGCGAG GGCGAGTTTC CTAGTGCCTC GGGCACCAGC 
CGGCCACGAG GAGGCATCAT AGACAGCCTG TTCGGCATCC CCGAGTCCAT ATCCGTCACC 
AATGCTGCCA CGACCCCCTG CCGAAATCCA AAGGATAGCA GTCTACGCAA GAAGAAAGCA 
GCCAACCTCA ACAACATCAT CCACCGGCTG GAGAAAGCGG CGAACCGAGA CGAGCCTCAC 
GAATGGGAGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cux1a ( XM_005172718.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172775.1 Danio rerio cut-like homeobox 1a (cux1a), transcript variant X1, mRNA.

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CloneID	ODa16296
Clone ID Related Accession (Same CDS sequence)	XM_005172718.3 , XM_005172718.4
Accession Version	XM_005172718.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	homeobox protein cut-like 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172718.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGAGAACCC AGGCCAGAAC CTGAACAGAG TGTTTCAGGA TGTTCCGAAG 
CGAAGAGGCG GCTCTGAAGG CTCCACGCGG CCAGGTAACA TCACCACACG GATCCGCACG 
CCTGAAACCG GCTCCGATGA AGCCATCAAG TCCATCTTAG AGCAGGCCAA GAGAGAGTTA 
CAAGTGCAGA AAGCAGACGG CGTGGGGGAG AGGCATCGGC GATCAGGTTA CCACAGAGGG 
TCGGGCAGCT ATTTCCAAAT ATATGCACAC AGTCTAAGAC TTGAAGAATC TAACATAGAT 
CTGAAACCCA TGAGGATGAC TAAGCAATTC GAGAGCTCTC AGCCTCCGTC CTCCTCCAGC 
AACTCGGACG AGAACATTCG CTCCATCCTC GAGCAGGCTC GCAGGGAGAT GGAGGCGCAG 
CAGGTGGCAC TGGAGCCCGT CTTGAAGGCC AGCTCCATGG GCCCGACGGA CCTCTCGCTC 
CTGGGCTCCA AACTCCTGGG CTCCCCGCTC TCCACCCTGC CCTACACCCC CATCACCCAG 
TCCCTGAAGA AGCCCTCTAG TTCACCGCTG CTGGACTTCC ACCATGGTGG AGTGAAGAAG 
GAGATGGGCG AGGTGGCCGT TTCGGATGTG GGTCTGGACG GAGTGGTGCC CAAGCTGGGC 
CGTTTGTCTG AGAGCGGTTT GGGTGGTGGC TCGTGGAGGG AGCACTGGTG GAGCACAGGC 
CTGTCAGAGC GCCGCGGGTC CAGCCAGCCC TCCGTTAGTG AAGACGCACA CAGTCAAGAG 
GACAGCAAAG AGAAGCTCCC TCGGCTGGGC AGCACTGCGC CGGTGGCTCC TGCGTCGTCT 
CTGGATTACT GGAAGGACTG GCCGAGCTCA GAATCGCCGT ACTCTCAGAG CTCAGAGCTC 
AGCCTGCAGA TGCCCAGTGG CAGTGAGACC CCTCAGAGCA GCCCACTGCC TTCCTCCTCG 
CCCTTACTGT CCCTGCCCAA AGTGGCCAAA CCTGTGGTGC CGCCGCTTAC GCCAGAGCAA 
TACGAGTACT ACATGTACCA GGAGGTGGAC ACTGTCGACC TCACACAGCA GGTCAAAGAA 
AAACTCGCCA AGAACGGCAT CTGCCAGCGC ATCTTCGGGG AGAAGGTGCT GGGTCTCTCT 
CAGGGGAGTG TGAGCGACAT GCTCTCTCGA CCAAAGCACT GGAGCAAACT CACACAGAAG 
GGAAGGGAGC CGTTTATACG CATGCAGTTA TGGCTGAACG GAGAGCTGGG ACAGGCACTG 
ATGCCAATGC CTGGACAGAC ACAAGAAATC ACCCCCAAAA CCTCGGCCAG CTGCAGTCCT 
GCCCCAGAAT CCCCCCTGAG CTCGGCTGAG GAGTCTGTGA AGAGCCAGCA GGAGGAGCAG 
ATGTGCCGAT CCTCCGTCCA GGAGCCCAGC GAAGCCTCCG AGTCTCAGCC CGGGACGCCG 
CTGCCGCTGC CTCTGCCGGG ACAGGCCGGT CTCAGCATTC AGGAGATGGT GGCCATGTCA 
GCCGAGCTGG ACACGTACGT CATCACGAAA AAAGTCAAAG AAGTCCTCAC TGATAACAAC 
CTGGGCCAGC GTCTGTTCGG GGAGAGCATT CTGGGTTTGA CGCAGGGCTC AGTGTCTGAT 
CTGCTGGCTC GACCCAAACC CTGGCACAAG CTCAGCCTGA AGGGTCGCGA GCCGTTCGTG 
CGCATGCAGC TGTGGTTGAG CGACCCGCGC AACGTGGAGA AGCTGATGGA CATGAAGCGC 
ATGGAGAAGA AAGCCTACAT GAAAAGGCGC CACAGCTCCA TCGGTGAGAG TCATTCGCTG 
GAGAGCGGCT TGTCTGCAGC AGACCTGACA CAGGGTGCCA GTCCCCAACA CCTATCCCAG 
CAGCAGCCGC CGCAGCAGAT GCAGCCTCAA CAGCAGCAGC CCCAGCTGAA GAAACCCCGG 
GTGGTTCTGG CTCCGGAAGA GAAGGAAGCT CTGAAGCGGG CCTACCAGCA GAAGCCTTAC 
CCCTCCCCTA AAACCATTGA GGAGTTAGCG AGTCAGCTCA ACCTCAAAAC CAGCACCGTC 
ATCAACTGGT TCCACAACTA CAGGTCTCGT ATCCGGCGAG AGCTTTTTAT CGAAGAGATC 
CAGGCAGCAG CAGGTGAAGG TGGATCTCCA TCTGCACGGC CTGGAAAATC TGGAGGCGAG 
GGAGAAAACA GCTGCGACGG CACTGAGTCC GACAGCACGG CAGAAGGCCG ACCGAGTGGC 
TCAGAGGAGC GCAAGGGCTT CCTGGAGAGC CCGGGCCTCG GCCACAGAGA AGAAGATGGC 
GAGGGCGAGT TTCCTAGTGC CTCGGGCACC AGCCGGCCAC GAGGAGGCAT CATAGACAGC 
CTGTTCGGCA TCCCCGAGTC CATATCCGTC ACCAATGCTG CCACGACCCC CTGCCGAAAT 
CCAAAGGATA GCAGTCTACG CAAGAAGAAA GCAGCCAACC TCAACAACAT CATCCACCGG 
CTGGAGAAAG CGGCGAACCG AGACGAGCCT CACGAATGGG AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172775.1
CDS	183..4670
Translation	MAANAGSMFQYWKRFDLQQLQKELDATATALANRQDESEQSRKKLIDQSREFKKNTPEDLRKQIAPLLKGFQAEIDALSKRSKESEAAFLSVYKRLIDVPDPVSALEAAQQLQVTVRKMHDLETENQRLKDTLQEYEREISEVKNQEVTIKALKEKLEQYERSLQAKNTEEAVEPNLDSSEREERQSKGENLPNNYADKESQPEQSQESRTEDSDVKTQSLQTALEATQAELHELKTKYDEESTAKANEIEAVMTDLERANQRAETAEREAGTLREQLTSAGKTMQSSNQIQNAPEMDKSLEAELGAKEREVERLAEDVQRLQASLAQLSETSTNQISQLEQQLSSKEALLKQLEEKLQDQSDYEEIKRELCTLKSVEHNSSDHHSSAQDSSKPLEMLLTGKDHSQLSESALKRLANSDFTGAVGRKLKETTVENPQRLPATPVPTTLQGSPPPPSPQLLMRTGTATSESAASANGTHPFSPTGIGPDFFTPGMASVGATFPPLAGKMALNSLLQRQLMQTIYTKALQDPSPSSVLFGPPHYATTNSLSSPLPPQSAGSPEVNGMAPSPSQSEGAGSTSEGEEMDTAEIARQVKEQLIKHNIGQRVFGHYVLGLSQGSVSEILARPKPWSKLTIRGKEPFHKMKHFLSDEQNILALRSIQGRQRENPGQNLNRVFQDVPKRRGGSEGSTRPGNITTRIRTPETGSDEAIKSILEQAKRELQVQKADGVGERHRRSGYHRGSGSYFQIYAHSLRLEESNIDLKPMRMTKQFESSQPPSSSSNSDENIRSILEQARREMEAQQVALEPVLKASSMGPTDLSLLGSKLLGSPLSTLPYTPITQSLKKPSSSPLLDFHHGGVKKEMGEVAVSDVGLDGVVPKLGRLSESGLGGGSWREHWWSTGLSERRGSSQPSVSEDAHSQEDSKEKLPRLGSTAPVAPASSLDYWKDWPSSESPYSQSSELSLQMPSGSETPQSSPLPSSSPLLSLPKVAKPVVPPLTPEQYEYYMYQEVDTVDLTQQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKHWSKLTQKGREPFIRMQLWLNGELGQALMPMPGQTQEITPKTSASCSPAPESPLSSAEESVKSQQEEQMCRSSVQEPSEASESQPGTPLPLPLPGQAGLSIQEMVAMSAELDTYVITKKVKEVLTDNNLGQRLFGESILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLSDPRNVEKLMDMKRMEKKAYMKRRHSSIGESHSLESGLSAADLTQGASPQHLSQQQPPQQMQPQQQQPQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEELASQLNLKTSTVINWFHNYRSRIRRELFIEEIQAAAGEGGSPSARPGKSGGEGENSCDGTESDSTAEGRPSGSEERKGFLESPGLGHREEDGEGEFPSASGTSRPRGGIIDSLFGIPESISVTNAATTPCRNPKDSSLRKKKAANLNNIIHRLEKAANRDEPHEWEF

Target ORF information:

RefSeq Version	XM_005172718.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172718.3

ORF Insert Sequence:

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGAGAACCC AGGCCAGAAC CTGAACAGAG TGTTTCAGGA TGTTCCGAAG 
CGAAGAGGCG GCTCTGAAGG CTCCACGCGG CCAGGTAACA TCACCACACG GATCCGCACG 
CCTGAAACCG GCTCCGATGA AGCCATCAAG TCCATCTTAG AGCAGGCCAA GAGAGAGTTA 
CAAGTGCAGA AAGCAGACGG CGTGGGGGAG AGGCATCGGC GATCAGGTTA CCACAGAGGG 
TCGGGCAGCT ATTTCCAAAT ATATGCACAC AGTCTAAGAC TTGAAGAATC TAACATAGAT 
CTGAAACCCA TGAGGATGAC TAAGCAATTC GAGAGCTCTC AGCCTCCGTC CTCCTCCAGC 
AACTCGGACG AGAACATTCG CTCCATCCTC GAGCAGGCTC GCAGGGAGAT GGAGGCGCAG 
CAGGTGGCAC TGGAGCCCGT CTTGAAGGCC AGCTCCATGG GCCCGACGGA CCTCTCGCTC 
CTGGGCTCCA AACTCCTGGG CTCCCCGCTC TCCACCCTGC CCTACACCCC CATCACCCAG 
TCCCTGAAGA AGCCCTCTAG TTCACCGCTG CTGGACTTCC ACCATGGTGG AGTGAAGAAG 
GAGATGGGCG AGGTGGCCGT TTCGGATGTG GGTCTGGACG GAGTGGTGCC CAAGCTGGGC 
CGTTTGTCTG AGAGCGGTTT GGGTGGTGGC TCGTGGAGGG AGCACTGGTG GAGCACAGGC 
CTGTCAGAGC GCCGCGGGTC CAGCCAGCCC TCCGTTAGTG AAGACGCACA CAGTCAAGAG 
GACAGCAAAG AGAAGCTCCC TCGGCTGGGC AGCACTGCGC CGGTGGCTCC TGCGTCGTCT 
CTGGATTACT GGAAGGACTG GCCGAGCTCA GAATCGCCGT ACTCTCAGAG CTCAGAGCTC 
AGCCTGCAGA TGCCCAGTGG CAGTGAGACC CCTCAGAGCA GCCCACTGCC TTCCTCCTCG 
CCCTTACTGT CCCTGCCCAA AGTGGCCAAA CCTGTGGTGC CGCCGCTTAC GCCAGAGCAA 
TACGAGTACT ACATGTACCA GGAGGTGGAC ACTGTCGACC TCACACAGCA GGTCAAAGAA 
AAACTCGCCA AGAACGGCAT CTGCCAGCGC ATCTTCGGGG AGAAGGTGCT GGGTCTCTCT 
CAGGGGAGTG TGAGCGACAT GCTCTCTCGA CCAAAGCACT GGAGCAAACT CACACAGAAG 
GGAAGGGAGC CGTTTATACG CATGCAGTTA TGGCTGAACG GAGAGCTGGG ACAGGCACTG 
ATGCCAATGC CTGGACAGAC ACAAGAAATC ACCCCCAAAA CCTCGGCCAG CTGCAGTCCT 
GCCCCAGAAT CCCCCCTGAG CTCGGCTGAG GAGTCTGTGA AGAGCCAGCA GGAGGAGCAG 
ATGTGCCGAT CCTCCGTCCA GGAGCCCAGC GAAGCCTCCG AGTCTCAGCC CGGGACGCCG 
CTGCCGCTGC CTCTGCCGGG ACAGGCCGGT CTCAGCATTC AGGAGATGGT GGCCATGTCA 
GCCGAGCTGG ACACGTACGT CATCACGAAA AAAGTCAAAG AAGTCCTCAC TGATAACAAC 
CTGGGCCAGC GTCTGTTCGG GGAGAGCATT CTGGGTTTGA CGCAGGGCTC AGTGTCTGAT 
CTGCTGGCTC GACCCAAACC CTGGCACAAG CTCAGCCTGA AGGGTCGCGA GCCGTTCGTG 
CGCATGCAGC TGTGGTTGAG CGACCCGCGC AACGTGGAGA AGCTGATGGA CATGAAGCGC 
ATGGAGAAGA AAGCCTACAT GAAAAGGCGC CACAGCTCCA TCGGTGAGAG TCATTCGCTG 
GAGAGCGGCT TGTCTGCAGC AGACCTGACA CAGGGTGCCA GTCCCCAACA CCTATCCCAG 
CAGCAGCCGC CGCAGCAGAT GCAGCCTCAA CAGCAGCAGC CCCAGCTGAA GAAACCCCGG 
GTGGTTCTGG CTCCGGAAGA GAAGGAAGCT CTGAAGCGGG CCTACCAGCA GAAGCCTTAC 
CCCTCCCCTA AAACCATTGA GGAGTTAGCG AGTCAGCTCA ACCTCAAAAC CAGCACCGTC 
ATCAACTGGT TCCACAACTA CAGGTCTCGT ATCCGGCGAG AGCTTTTTAT CGAAGAGATC 
CAGGCAGCAG CAGGTGAAGG TGGATCTCCA TCTGCACGGC CTGGAAAATC TGGAGGCGAG 
GGAGAAAACA GCTGCGACGG CACTGAGTCC GACAGCACGG CAGAAGGCCG ACCGAGTGGC 
TCAGAGGAGC GCAAGGGCTT CCTGGAGAGC CCGGGCCTCG GCCACAGAGA AGAAGATGGC 
GAGGGCGAGT TTCCTAGTGC CTCGGGCACC AGCCGGCCAC GAGGAGGCAT CATAGACAGC 
CTGTTCGGCA TCCCCGAGTC CATATCCGTC ACCAATGCTG CCACGACCCC CTGCCGAAAT 
CCAAAGGATA GCAGTCTACG CAAGAAGAAA GCAGCCAACC TCAACAACAT CATCCACCGG 
CTGGAGAAAG CGGCGAACCG AGACGAGCCT CACGAATGGG AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cux1a ( XM_005172718.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172775.1 Danio rerio cut-like homeobox 1a (cux1a), transcript variant X1, mRNA.

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CloneID	ODa16296
Clone ID Related Accession (Same CDS sequence)	XM_005172718.3 , XM_005172718.4
Accession Version	XM_005172718.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	homeobox protein cut-like 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172718.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGAGAACCC AGGCCAGAAC CTGAACAGAG TGTTTCAGGA TGTTCCGAAG 
CGAAGAGGCG GCTCTGAAGG CTCCACGCGG CCAGGTAACA TCACCACACG GATCCGCACG 
CCTGAAACCG GCTCCGATGA AGCCATCAAG TCCATCTTAG AGCAGGCCAA GAGAGAGTTA 
CAAGTGCAGA AAGCAGACGG CGTGGGGGAG AGGCATCGGC GATCAGGTTA CCACAGAGGG 
TCGGGCAGCT ATTTCCAAAT ATATGCACAC AGTCTAAGAC TTGAAGAATC TAACATAGAT 
CTGAAACCCA TGAGGATGAC TAAGCAATTC GAGAGCTCTC AGCCTCCGTC CTCCTCCAGC 
AACTCGGACG AGAACATTCG CTCCATCCTC GAGCAGGCTC GCAGGGAGAT GGAGGCGCAG 
CAGGTGGCAC TGGAGCCCGT CTTGAAGGCC AGCTCCATGG GCCCGACGGA CCTCTCGCTC 
CTGGGCTCCA AACTCCTGGG CTCCCCGCTC TCCACCCTGC CCTACACCCC CATCACCCAG 
TCCCTGAAGA AGCCCTCTAG TTCACCGCTG CTGGACTTCC ACCATGGTGG AGTGAAGAAG 
GAGATGGGCG AGGTGGCCGT TTCGGATGTG GGTCTGGACG GAGTGGTGCC CAAGCTGGGC 
CGTTTGTCTG AGAGCGGTTT GGGTGGTGGC TCGTGGAGGG AGCACTGGTG GAGCACAGGC 
CTGTCAGAGC GCCGCGGGTC CAGCCAGCCC TCCGTTAGTG AAGACGCACA CAGTCAAGAG 
GACAGCAAAG AGAAGCTCCC TCGGCTGGGC AGCACTGCGC CGGTGGCTCC TGCGTCGTCT 
CTGGATTACT GGAAGGACTG GCCGAGCTCA GAATCGCCGT ACTCTCAGAG CTCAGAGCTC 
AGCCTGCAGA TGCCCAGTGG CAGTGAGACC CCTCAGAGCA GCCCACTGCC TTCCTCCTCG 
CCCTTACTGT CCCTGCCCAA AGTGGCCAAA CCTGTGGTGC CGCCGCTTAC GCCAGAGCAA 
TACGAGTACT ACATGTACCA GGAGGTGGAC ACTGTCGACC TCACACAGCA GGTCAAAGAA 
AAACTCGCCA AGAACGGCAT CTGCCAGCGC ATCTTCGGGG AGAAGGTGCT GGGTCTCTCT 
CAGGGGAGTG TGAGCGACAT GCTCTCTCGA CCAAAGCACT GGAGCAAACT CACACAGAAG 
GGAAGGGAGC CGTTTATACG CATGCAGTTA TGGCTGAACG GAGAGCTGGG ACAGGCACTG 
ATGCCAATGC CTGGACAGAC ACAAGAAATC ACCCCCAAAA CCTCGGCCAG CTGCAGTCCT 
GCCCCAGAAT CCCCCCTGAG CTCGGCTGAG GAGTCTGTGA AGAGCCAGCA GGAGGAGCAG 
ATGTGCCGAT CCTCCGTCCA GGAGCCCAGC GAAGCCTCCG AGTCTCAGCC CGGGACGCCG 
CTGCCGCTGC CTCTGCCGGG ACAGGCCGGT CTCAGCATTC AGGAGATGGT GGCCATGTCA 
GCCGAGCTGG ACACGTACGT CATCACGAAA AAAGTCAAAG AAGTCCTCAC TGATAACAAC 
CTGGGCCAGC GTCTGTTCGG GGAGAGCATT CTGGGTTTGA CGCAGGGCTC AGTGTCTGAT 
CTGCTGGCTC GACCCAAACC CTGGCACAAG CTCAGCCTGA AGGGTCGCGA GCCGTTCGTG 
CGCATGCAGC TGTGGTTGAG CGACCCGCGC AACGTGGAGA AGCTGATGGA CATGAAGCGC 
ATGGAGAAGA AAGCCTACAT GAAAAGGCGC CACAGCTCCA TCGGTGAGAG TCATTCGCTG 
GAGAGCGGCT TGTCTGCAGC AGACCTGACA CAGGGTGCCA GTCCCCAACA CCTATCCCAG 
CAGCAGCCGC CGCAGCAGAT GCAGCCTCAA CAGCAGCAGC CCCAGCTGAA GAAACCCCGG 
GTGGTTCTGG CTCCGGAAGA GAAGGAAGCT CTGAAGCGGG CCTACCAGCA GAAGCCTTAC 
CCCTCCCCTA AAACCATTGA GGAGTTAGCG AGTCAGCTCA ACCTCAAAAC CAGCACCGTC 
ATCAACTGGT TCCACAACTA CAGGTCTCGT ATCCGGCGAG AGCTTTTTAT CGAAGAGATC 
CAGGCAGCAG CAGGTGAAGG TGGATCTCCA TCTGCACGGC CTGGAAAATC TGGAGGCGAG 
GGAGAAAACA GCTGCGACGG CACTGAGTCC GACAGCACGG CAGAAGGCCG ACCGAGTGGC 
TCAGAGGAGC GCAAGGGCTT CCTGGAGAGC CCGGGCCTCG GCCACAGAGA AGAAGATGGC 
GAGGGCGAGT TTCCTAGTGC CTCGGGCACC AGCCGGCCAC GAGGAGGCAT CATAGACAGC 
CTGTTCGGCA TCCCCGAGTC CATATCCGTC ACCAATGCTG CCACGACCCC CTGCCGAAAT 
CCAAAGGATA GCAGTCTACG CAAGAAGAAA GCAGCCAACC TCAACAACAT CATCCACCGG 
CTGGAGAAAG CGGCGAACCG AGACGAGCCT CACGAATGGG AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172775.1
CDS	79..4566
Translation	MAANAGSMFQYWKRFDLQQLQKELDATATALANRQDESEQSRKKLIDQSREFKKNTPEDLRKQIAPLLKGFQAEIDALSKRSKESEAAFLSVYKRLIDVPDPVSALEAAQQLQVTVRKMHDLETENQRLKDTLQEYEREISEVKNQEVTIKALKEKLEQYERSLQAKNTEEAVEPNLDSSEREERQSKGENLPNNYADKESQPEQSQESRTEDSDVKTQSLQTALEATQAELHELKTKYDEESTAKANEIEAVMTDLERANQRAETAEREAGTLREQLTSAGKTMQSSNQIQNAPEMDKSLEAELGAKEREVERLAEDVQRLQASLAQLSETSTNQISQLEQQLSSKEALLKQLEEKLQDQSDYEEIKRELCTLKSVEHNSSDHHSSAQDSSKPLEMLLTGKDHSQLSESALKRLANSDFTGAVGRKLKETTVENPQRLPATPVPTTLQGSPPPPSPQLLMRTGTATSESAASANGTHPFSPTGIGPDFFTPGMASVGATFPPLAGKMALNSLLQRQLMQTIYTKALQDPSPSSVLFGPPHYATTNSLSSPLPPQSAGSPEVNGMAPSPSQSEGAGSTSEGEEMDTAEIARQVKEQLIKHNIGQRVFGHYVLGLSQGSVSEILARPKPWSKLTIRGKEPFHKMKHFLSDEQNILALRSIQGRQRENPGQNLNRVFQDVPKRRGGSEGSTRPGNITTRIRTPETGSDEAIKSILEQAKRELQVQKADGVGERHRRSGYHRGSGSYFQIYAHSLRLEESNIDLKPMRMTKQFESSQPPSSSSNSDENIRSILEQARREMEAQQVALEPVLKASSMGPTDLSLLGSKLLGSPLSTLPYTPITQSLKKPSSSPLLDFHHGGVKKEMGEVAVSDVGLDGVVPKLGRLSESGLGGGSWREHWWSTGLSERRGSSQPSVSEDAHSQEDSKEKLPRLGSTAPVAPASSLDYWKDWPSSESPYSQSSELSLQMPSGSETPQSSPLPSSSPLLSLPKVAKPVVPPLTPEQYEYYMYQEVDTVDLTQQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKHWSKLTQKGREPFIRMQLWLNGELGQALMPMPGQTQEITPKTSASCSPAPESPLSSAEESVKSQQEEQMCRSSVQEPSEASESQPGTPLPLPLPGQAGLSIQEMVAMSAELDTYVITKKVKEVLTDNNLGQRLFGESILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLSDPRNVEKLMDMKRMEKKAYMKRRHSSIGESHSLESGLSAADLTQGASPQHLSQQQPPQQMQPQQQQPQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEELASQLNLKTSTVINWFHNYRSRIRRELFIEEIQAAAGEGGSPSARPGKSGGEGENSCDGTESDSTAEGRPSGSEERKGFLESPGLGHREEDGEGEFPSASGTSRPRGGIIDSLFGIPESISVTNAATTPCRNPKDSSLRKKKAANLNNIIHRLEKAANRDEPHEWEF

Target ORF information:

RefSeq Version	XM_005172718.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172718.4

ORF Insert Sequence:

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGAGAACCC AGGCCAGAAC CTGAACAGAG TGTTTCAGGA TGTTCCGAAG 
CGAAGAGGCG GCTCTGAAGG CTCCACGCGG CCAGGTAACA TCACCACACG GATCCGCACG 
CCTGAAACCG GCTCCGATGA AGCCATCAAG TCCATCTTAG AGCAGGCCAA GAGAGAGTTA 
CAAGTGCAGA AAGCAGACGG CGTGGGGGAG AGGCATCGGC GATCAGGTTA CCACAGAGGG 
TCGGGCAGCT ATTTCCAAAT ATATGCACAC AGTCTAAGAC TTGAAGAATC TAACATAGAT 
CTGAAACCCA TGAGGATGAC TAAGCAATTC GAGAGCTCTC AGCCTCCGTC CTCCTCCAGC 
AACTCGGACG AGAACATTCG CTCCATCCTC GAGCAGGCTC GCAGGGAGAT GGAGGCGCAG 
CAGGTGGCAC TGGAGCCCGT CTTGAAGGCC AGCTCCATGG GCCCGACGGA CCTCTCGCTC 
CTGGGCTCCA AACTCCTGGG CTCCCCGCTC TCCACCCTGC CCTACACCCC CATCACCCAG 
TCCCTGAAGA AGCCCTCTAG TTCACCGCTG CTGGACTTCC ACCATGGTGG AGTGAAGAAG 
GAGATGGGCG AGGTGGCCGT TTCGGATGTG GGTCTGGACG GAGTGGTGCC CAAGCTGGGC 
CGTTTGTCTG AGAGCGGTTT GGGTGGTGGC TCGTGGAGGG AGCACTGGTG GAGCACAGGC 
CTGTCAGAGC GCCGCGGGTC CAGCCAGCCC TCCGTTAGTG AAGACGCACA CAGTCAAGAG 
GACAGCAAAG AGAAGCTCCC TCGGCTGGGC AGCACTGCGC CGGTGGCTCC TGCGTCGTCT 
CTGGATTACT GGAAGGACTG GCCGAGCTCA GAATCGCCGT ACTCTCAGAG CTCAGAGCTC 
AGCCTGCAGA TGCCCAGTGG CAGTGAGACC CCTCAGAGCA GCCCACTGCC TTCCTCCTCG 
CCCTTACTGT CCCTGCCCAA AGTGGCCAAA CCTGTGGTGC CGCCGCTTAC GCCAGAGCAA 
TACGAGTACT ACATGTACCA GGAGGTGGAC ACTGTCGACC TCACACAGCA GGTCAAAGAA 
AAACTCGCCA AGAACGGCAT CTGCCAGCGC ATCTTCGGGG AGAAGGTGCT GGGTCTCTCT 
CAGGGGAGTG TGAGCGACAT GCTCTCTCGA CCAAAGCACT GGAGCAAACT CACACAGAAG 
GGAAGGGAGC CGTTTATACG CATGCAGTTA TGGCTGAACG GAGAGCTGGG ACAGGCACTG 
ATGCCAATGC CTGGACAGAC ACAAGAAATC ACCCCCAAAA CCTCGGCCAG CTGCAGTCCT 
GCCCCAGAAT CCCCCCTGAG CTCGGCTGAG GAGTCTGTGA AGAGCCAGCA GGAGGAGCAG 
ATGTGCCGAT CCTCCGTCCA GGAGCCCAGC GAAGCCTCCG AGTCTCAGCC CGGGACGCCG 
CTGCCGCTGC CTCTGCCGGG ACAGGCCGGT CTCAGCATTC AGGAGATGGT GGCCATGTCA 
GCCGAGCTGG ACACGTACGT CATCACGAAA AAAGTCAAAG AAGTCCTCAC TGATAACAAC 
CTGGGCCAGC GTCTGTTCGG GGAGAGCATT CTGGGTTTGA CGCAGGGCTC AGTGTCTGAT 
CTGCTGGCTC GACCCAAACC CTGGCACAAG CTCAGCCTGA AGGGTCGCGA GCCGTTCGTG 
CGCATGCAGC TGTGGTTGAG CGACCCGCGC AACGTGGAGA AGCTGATGGA CATGAAGCGC 
ATGGAGAAGA AAGCCTACAT GAAAAGGCGC CACAGCTCCA TCGGTGAGAG TCATTCGCTG 
GAGAGCGGCT TGTCTGCAGC AGACCTGACA CAGGGTGCCA GTCCCCAACA CCTATCCCAG 
CAGCAGCCGC CGCAGCAGAT GCAGCCTCAA CAGCAGCAGC CCCAGCTGAA GAAACCCCGG 
GTGGTTCTGG CTCCGGAAGA GAAGGAAGCT CTGAAGCGGG CCTACCAGCA GAAGCCTTAC 
CCCTCCCCTA AAACCATTGA GGAGTTAGCG AGTCAGCTCA ACCTCAAAAC CAGCACCGTC 
ATCAACTGGT TCCACAACTA CAGGTCTCGT ATCCGGCGAG AGCTTTTTAT CGAAGAGATC 
CAGGCAGCAG CAGGTGAAGG TGGATCTCCA TCTGCACGGC CTGGAAAATC TGGAGGCGAG 
GGAGAAAACA GCTGCGACGG CACTGAGTCC GACAGCACGG CAGAAGGCCG ACCGAGTGGC 
TCAGAGGAGC GCAAGGGCTT CCTGGAGAGC CCGGGCCTCG GCCACAGAGA AGAAGATGGC 
GAGGGCGAGT TTCCTAGTGC CTCGGGCACC AGCCGGCCAC GAGGAGGCAT CATAGACAGC 
CTGTTCGGCA TCCCCGAGTC CATATCCGTC ACCAATGCTG CCACGACCCC CTGCCGAAAT 
CCAAAGGATA GCAGTCTACG CAAGAAGAAA GCAGCCAACC TCAACAACAT CATCCACCGG 
CTGGAGAAAG CGGCGAACCG AGACGAGCCT CACGAATGGG AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cux1a ( XM_005172719.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172776.1 Danio rerio cut-like homeobox 1a (cux1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa16297
Clone ID Related Accession (Same CDS sequence)	XM_005172719.4 , XM_005172719.3
Accession Version	XM_005172719.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	homeobox protein cut-like 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172719.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGAGAACCC AGGCCAGAAC CTGAACAGAG TGTTTCAGGA TGTTCCGAAG 
CGAAGAGGCG GCTCTGAAGG CTCCACGCGG CCAGGTAACA TCACCACACG GATCCGCACG 
CCTGAAACCG GCTCCGATGA AGCCATCAAG TCCATCTTAG AGCAGGCCAA GAGAGAGTTA 
CAAGTGCAGA AAGCAGACGG CGTGGGGGAG AGGCATCGGC GATCAGGTTA CCACAGAGGG 
TCGGGCAGCT ATTTCCAAAT ATATGCACAC AGTCTAAGAC TTGTCGAGAG CTCTCAGCCT 
CCGTCCTCCT CCAGCAACTC GGACGAGAAC ATTCGCTCCA TCCTCGAGCA GGCTCGCAGG 
GAGATGGAGG CGCAGCAGGT GGCACTGGAG CCCGTCTTGA AGGCCAGCTC CATGGGCCCG 
ACGGACCTCT CGCTCCTGGG CTCCAAACTC CTGGGCTCCC CGCTCTCCAC CCTGCCCTAC 
ACCCCCATCA CCCAGTCCCT GAAGAAGCCC TCTAGTTCAC CGCTGCTGGA CTTCCACCAT 
GGTGGAGTGA AGAAGGAGAT GGGCGAGGTG GCCGTTTCGG ATGTGGGTCT GGACGGAGTG 
GTGCCCAAGC TGGGCCGTTT GTCTGAGAGC GGTTTGGGTG GTGGCTCGTG GAGGGAGCAC 
TGGTGGAGCA CAGGCCTGTC AGAGCGCCGC GGGTCCAGCC AGCCCTCCGT TAGTGAAGAC 
GCACACAGTC AAGAGGACAG CAAAGAGAAG CTCCCTCGGC TGGGCAGCAC TGCGCCGGTG 
GCTCCTGCGT CGTCTCTGGA TTACTGGAAG GACTGGCCGA GCTCAGAATC GCCGTACTCT 
CAGAGCTCAG AGCTCAGCCT GCAGATGCCC AGTGGCAGTG AGACCCCTCA GAGCAGCCCA 
CTGCCTTCCT CCTCGCCCTT ACTGTCCCTG CCCAAAGTGG CCAAACCTGT GGTGCCGCCG 
CTTACGCCAG AGCAATACGA GTACTACATG TACCAGGAGG TGGACACTGT CGACCTCACA 
CAGCAGGTCA AAGAAAAACT CGCCAAGAAC GGCATCTGCC AGCGCATCTT CGGGGAGAAG 
GTGCTGGGTC TCTCTCAGGG GAGTGTGAGC GACATGCTCT CTCGACCAAA GCACTGGAGC 
AAACTCACAC AGAAGGGAAG GGAGCCGTTT ATACGCATGC AGTTATGGCT GAACGGAGAG 
CTGGGACAGG CACTGATGCC AATGCCTGGA CAGACACAAG AAATCACCCC CAAAACCTCG 
GCCAGCTGCA GTCCTGCCCC AGAATCCCCC CTGAGCTCGG CTGAGGAGTC TGTGAAGAGC 
CAGCAGGAGG AGCAGATGTG CCGATCCTCC GTCCAGGAGC CCAGCGAAGC CTCCGAGTCT 
CAGCCCGGGA CGCCGCTGCC GCTGCCTCTG CCGGGACAGG CCGGTCTCAG CATTCAGGAG 
ATGGTGGCCA TGTCAGCCGA GCTGGACACG TACGTCATCA CGAAAAAAGT CAAAGAAGTC 
CTCACTGATA ACAACCTGGG CCAGCGTCTG TTCGGGGAGA GCATTCTGGG TTTGACGCAG 
GGCTCAGTGT CTGATCTGCT GGCTCGACCC AAACCCTGGC ACAAGCTCAG CCTGAAGGGT 
CGCGAGCCGT TCGTGCGCAT GCAGCTGTGG TTGAGCGACC CGCGCAACGT GGAGAAGCTG 
ATGGACATGA AGCGCATGGA GAAGAAAGCC TACATGAAAA GGCGCCACAG CTCCATCGGT 
GAGAGTCATT CGCTGGAGAG CGGCTTGTCT GCAGCAGACC TGACACAGGG TGCCAGTCCC 
CAACACCTAT CCCAGCAGCA GCCGCCGCAG CAGATGCAGC CTCAACAGCA GCAGCCCCAG 
CTGAAGAAAC CCCGGGTGGT TCTGGCTCCG GAAGAGAAGG AAGCTCTGAA GCGGGCCTAC 
CAGCAGAAGC CTTACCCCTC CCCTAAAACC ATTGAGGAGT TAGCGAGTCA GCTCAACCTC 
AAAACCAGCA CCGTCATCAA CTGGTTCCAC AACTACAGGT CTCGTATCCG GCGAGAGCTT 
TTTATCGAAG AGATCCAGGC AGCAGCAGGT GAAGGTGGAT CTCCATCTGC ACGGCCTGGA 
AAATCTGGAG GCGAGGGAGA AAACAGCTGC GACGGCACTG AGTCCGACAG CACGGCAGAA 
GGCCGACCGA GTGGCTCAGA GGAGCGCAAG GGCTTCCTGG AGAGCCCGGG CCTCGGCCAC 
AGAGAAGAAG ATGGCGAGGG CGAGTTTCCT AGTGCCTCGG GCACCAGCCG GCCACGAGGA 
GGCATCATAG ACAGCCTGTT CGGCATCCCC GAGTCCATAT CCGTCACCAA TGCTGCCACG 
ACCCCCTGCC GAAATCCAAA GGATAGCAGT CTACGCAAGA AGAAAGCAGC CAACCTCAAC 
AACATCATCC ACCGGCTGGA GAAAGCGGCG AACCGAGACG AGCCTCACGA ATGGGAGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172776.1
CDS	79..4521
Translation	MAANAGSMFQYWKRFDLQQLQKELDATATALANRQDESEQSRKKLIDQSREFKKNTPEDLRKQIAPLLKGFQAEIDALSKRSKESEAAFLSVYKRLIDVPDPVSALEAAQQLQVTVRKMHDLETENQRLKDTLQEYEREISEVKNQEVTIKALKEKLEQYERSLQAKNTEEAVEPNLDSSEREERQSKGENLPNNYADKESQPEQSQESRTEDSDVKTQSLQTALEATQAELHELKTKYDEESTAKANEIEAVMTDLERANQRAETAEREAGTLREQLTSAGKTMQSSNQIQNAPEMDKSLEAELGAKEREVERLAEDVQRLQASLAQLSETSTNQISQLEQQLSSKEALLKQLEEKLQDQSDYEEIKRELCTLKSVEHNSSDHHSSAQDSSKPLEMLLTGKDHSQLSESALKRLANSDFTGAVGRKLKETTVENPQRLPATPVPTTLQGSPPPPSPQLLMRTGTATSESAASANGTHPFSPTGIGPDFFTPGMASVGATFPPLAGKMALNSLLQRQLMQTIYTKALQDPSPSSVLFGPPHYATTNSLSSPLPPQSAGSPEVNGMAPSPSQSEGAGSTSEGEEMDTAEIARQVKEQLIKHNIGQRVFGHYVLGLSQGSVSEILARPKPWSKLTIRGKEPFHKMKHFLSDEQNILALRSIQGRQRENPGQNLNRVFQDVPKRRGGSEGSTRPGNITTRIRTPETGSDEAIKSILEQAKRELQVQKADGVGERHRRSGYHRGSGSYFQIYAHSLRLVESSQPPSSSSNSDENIRSILEQARREMEAQQVALEPVLKASSMGPTDLSLLGSKLLGSPLSTLPYTPITQSLKKPSSSPLLDFHHGGVKKEMGEVAVSDVGLDGVVPKLGRLSESGLGGGSWREHWWSTGLSERRGSSQPSVSEDAHSQEDSKEKLPRLGSTAPVAPASSLDYWKDWPSSESPYSQSSELSLQMPSGSETPQSSPLPSSSPLLSLPKVAKPVVPPLTPEQYEYYMYQEVDTVDLTQQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKHWSKLTQKGREPFIRMQLWLNGELGQALMPMPGQTQEITPKTSASCSPAPESPLSSAEESVKSQQEEQMCRSSVQEPSEASESQPGTPLPLPLPGQAGLSIQEMVAMSAELDTYVITKKVKEVLTDNNLGQRLFGESILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLSDPRNVEKLMDMKRMEKKAYMKRRHSSIGESHSLESGLSAADLTQGASPQHLSQQQPPQQMQPQQQQPQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEELASQLNLKTSTVINWFHNYRSRIRRELFIEEIQAAAGEGGSPSARPGKSGGEGENSCDGTESDSTAEGRPSGSEERKGFLESPGLGHREEDGEGEFPSASGTSRPRGGIIDSLFGIPESISVTNAATTPCRNPKDSSLRKKKAANLNNIIHRLEKAANRDEPHEWEF

Target ORF information:

RefSeq Version	XM_005172719.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172719.4

ORF Insert Sequence:

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGAGAACCC AGGCCAGAAC CTGAACAGAG TGTTTCAGGA TGTTCCGAAG 
CGAAGAGGCG GCTCTGAAGG CTCCACGCGG CCAGGTAACA TCACCACACG GATCCGCACG 
CCTGAAACCG GCTCCGATGA AGCCATCAAG TCCATCTTAG AGCAGGCCAA GAGAGAGTTA 
CAAGTGCAGA AAGCAGACGG CGTGGGGGAG AGGCATCGGC GATCAGGTTA CCACAGAGGG 
TCGGGCAGCT ATTTCCAAAT ATATGCACAC AGTCTAAGAC TTGTCGAGAG CTCTCAGCCT 
CCGTCCTCCT CCAGCAACTC GGACGAGAAC ATTCGCTCCA TCCTCGAGCA GGCTCGCAGG 
GAGATGGAGG CGCAGCAGGT GGCACTGGAG CCCGTCTTGA AGGCCAGCTC CATGGGCCCG 
ACGGACCTCT CGCTCCTGGG CTCCAAACTC CTGGGCTCCC CGCTCTCCAC CCTGCCCTAC 
ACCCCCATCA CCCAGTCCCT GAAGAAGCCC TCTAGTTCAC CGCTGCTGGA CTTCCACCAT 
GGTGGAGTGA AGAAGGAGAT GGGCGAGGTG GCCGTTTCGG ATGTGGGTCT GGACGGAGTG 
GTGCCCAAGC TGGGCCGTTT GTCTGAGAGC GGTTTGGGTG GTGGCTCGTG GAGGGAGCAC 
TGGTGGAGCA CAGGCCTGTC AGAGCGCCGC GGGTCCAGCC AGCCCTCCGT TAGTGAAGAC 
GCACACAGTC AAGAGGACAG CAAAGAGAAG CTCCCTCGGC TGGGCAGCAC TGCGCCGGTG 
GCTCCTGCGT CGTCTCTGGA TTACTGGAAG GACTGGCCGA GCTCAGAATC GCCGTACTCT 
CAGAGCTCAG AGCTCAGCCT GCAGATGCCC AGTGGCAGTG AGACCCCTCA GAGCAGCCCA 
CTGCCTTCCT CCTCGCCCTT ACTGTCCCTG CCCAAAGTGG CCAAACCTGT GGTGCCGCCG 
CTTACGCCAG AGCAATACGA GTACTACATG TACCAGGAGG TGGACACTGT CGACCTCACA 
CAGCAGGTCA AAGAAAAACT CGCCAAGAAC GGCATCTGCC AGCGCATCTT CGGGGAGAAG 
GTGCTGGGTC TCTCTCAGGG GAGTGTGAGC GACATGCTCT CTCGACCAAA GCACTGGAGC 
AAACTCACAC AGAAGGGAAG GGAGCCGTTT ATACGCATGC AGTTATGGCT GAACGGAGAG 
CTGGGACAGG CACTGATGCC AATGCCTGGA CAGACACAAG AAATCACCCC CAAAACCTCG 
GCCAGCTGCA GTCCTGCCCC AGAATCCCCC CTGAGCTCGG CTGAGGAGTC TGTGAAGAGC 
CAGCAGGAGG AGCAGATGTG CCGATCCTCC GTCCAGGAGC CCAGCGAAGC CTCCGAGTCT 
CAGCCCGGGA CGCCGCTGCC GCTGCCTCTG CCGGGACAGG CCGGTCTCAG CATTCAGGAG 
ATGGTGGCCA TGTCAGCCGA GCTGGACACG TACGTCATCA CGAAAAAAGT CAAAGAAGTC 
CTCACTGATA ACAACCTGGG CCAGCGTCTG TTCGGGGAGA GCATTCTGGG TTTGACGCAG 
GGCTCAGTGT CTGATCTGCT GGCTCGACCC AAACCCTGGC ACAAGCTCAG CCTGAAGGGT 
CGCGAGCCGT TCGTGCGCAT GCAGCTGTGG TTGAGCGACC CGCGCAACGT GGAGAAGCTG 
ATGGACATGA AGCGCATGGA GAAGAAAGCC TACATGAAAA GGCGCCACAG CTCCATCGGT 
GAGAGTCATT CGCTGGAGAG CGGCTTGTCT GCAGCAGACC TGACACAGGG TGCCAGTCCC 
CAACACCTAT CCCAGCAGCA GCCGCCGCAG CAGATGCAGC CTCAACAGCA GCAGCCCCAG 
CTGAAGAAAC CCCGGGTGGT TCTGGCTCCG GAAGAGAAGG AAGCTCTGAA GCGGGCCTAC 
CAGCAGAAGC CTTACCCCTC CCCTAAAACC ATTGAGGAGT TAGCGAGTCA GCTCAACCTC 
AAAACCAGCA CCGTCATCAA CTGGTTCCAC AACTACAGGT CTCGTATCCG GCGAGAGCTT 
TTTATCGAAG AGATCCAGGC AGCAGCAGGT GAAGGTGGAT CTCCATCTGC ACGGCCTGGA 
AAATCTGGAG GCGAGGGAGA AAACAGCTGC GACGGCACTG AGTCCGACAG CACGGCAGAA 
GGCCGACCGA GTGGCTCAGA GGAGCGCAAG GGCTTCCTGG AGAGCCCGGG CCTCGGCCAC 
AGAGAAGAAG ATGGCGAGGG CGAGTTTCCT AGTGCCTCGG GCACCAGCCG GCCACGAGGA 
GGCATCATAG ACAGCCTGTT CGGCATCCCC GAGTCCATAT CCGTCACCAA TGCTGCCACG 
ACCCCCTGCC GAAATCCAAA GGATAGCAGT CTACGCAAGA AGAAAGCAGC CAACCTCAAC 
AACATCATCC ACCGGCTGGA GAAAGCGGCG AACCGAGACG AGCCTCACGA ATGGGAGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cux1a ( XM_005172719.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172776.1 Danio rerio cut-like homeobox 1a (cux1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa16297
Clone ID Related Accession (Same CDS sequence)	XM_005172719.4 , XM_005172719.3
Accession Version	XM_005172719.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	homeobox protein cut-like 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172719.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGAGAACCC AGGCCAGAAC CTGAACAGAG TGTTTCAGGA TGTTCCGAAG 
CGAAGAGGCG GCTCTGAAGG CTCCACGCGG CCAGGTAACA TCACCACACG GATCCGCACG 
CCTGAAACCG GCTCCGATGA AGCCATCAAG TCCATCTTAG AGCAGGCCAA GAGAGAGTTA 
CAAGTGCAGA AAGCAGACGG CGTGGGGGAG AGGCATCGGC GATCAGGTTA CCACAGAGGG 
TCGGGCAGCT ATTTCCAAAT ATATGCACAC AGTCTAAGAC TTGTCGAGAG CTCTCAGCCT 
CCGTCCTCCT CCAGCAACTC GGACGAGAAC ATTCGCTCCA TCCTCGAGCA GGCTCGCAGG 
GAGATGGAGG CGCAGCAGGT GGCACTGGAG CCCGTCTTGA AGGCCAGCTC CATGGGCCCG 
ACGGACCTCT CGCTCCTGGG CTCCAAACTC CTGGGCTCCC CGCTCTCCAC CCTGCCCTAC 
ACCCCCATCA CCCAGTCCCT GAAGAAGCCC TCTAGTTCAC CGCTGCTGGA CTTCCACCAT 
GGTGGAGTGA AGAAGGAGAT GGGCGAGGTG GCCGTTTCGG ATGTGGGTCT GGACGGAGTG 
GTGCCCAAGC TGGGCCGTTT GTCTGAGAGC GGTTTGGGTG GTGGCTCGTG GAGGGAGCAC 
TGGTGGAGCA CAGGCCTGTC AGAGCGCCGC GGGTCCAGCC AGCCCTCCGT TAGTGAAGAC 
GCACACAGTC AAGAGGACAG CAAAGAGAAG CTCCCTCGGC TGGGCAGCAC TGCGCCGGTG 
GCTCCTGCGT CGTCTCTGGA TTACTGGAAG GACTGGCCGA GCTCAGAATC GCCGTACTCT 
CAGAGCTCAG AGCTCAGCCT GCAGATGCCC AGTGGCAGTG AGACCCCTCA GAGCAGCCCA 
CTGCCTTCCT CCTCGCCCTT ACTGTCCCTG CCCAAAGTGG CCAAACCTGT GGTGCCGCCG 
CTTACGCCAG AGCAATACGA GTACTACATG TACCAGGAGG TGGACACTGT CGACCTCACA 
CAGCAGGTCA AAGAAAAACT CGCCAAGAAC GGCATCTGCC AGCGCATCTT CGGGGAGAAG 
GTGCTGGGTC TCTCTCAGGG GAGTGTGAGC GACATGCTCT CTCGACCAAA GCACTGGAGC 
AAACTCACAC AGAAGGGAAG GGAGCCGTTT ATACGCATGC AGTTATGGCT GAACGGAGAG 
CTGGGACAGG CACTGATGCC AATGCCTGGA CAGACACAAG AAATCACCCC CAAAACCTCG 
GCCAGCTGCA GTCCTGCCCC AGAATCCCCC CTGAGCTCGG CTGAGGAGTC TGTGAAGAGC 
CAGCAGGAGG AGCAGATGTG CCGATCCTCC GTCCAGGAGC CCAGCGAAGC CTCCGAGTCT 
CAGCCCGGGA CGCCGCTGCC GCTGCCTCTG CCGGGACAGG CCGGTCTCAG CATTCAGGAG 
ATGGTGGCCA TGTCAGCCGA GCTGGACACG TACGTCATCA CGAAAAAAGT CAAAGAAGTC 
CTCACTGATA ACAACCTGGG CCAGCGTCTG TTCGGGGAGA GCATTCTGGG TTTGACGCAG 
GGCTCAGTGT CTGATCTGCT GGCTCGACCC AAACCCTGGC ACAAGCTCAG CCTGAAGGGT 
CGCGAGCCGT TCGTGCGCAT GCAGCTGTGG TTGAGCGACC CGCGCAACGT GGAGAAGCTG 
ATGGACATGA AGCGCATGGA GAAGAAAGCC TACATGAAAA GGCGCCACAG CTCCATCGGT 
GAGAGTCATT CGCTGGAGAG CGGCTTGTCT GCAGCAGACC TGACACAGGG TGCCAGTCCC 
CAACACCTAT CCCAGCAGCA GCCGCCGCAG CAGATGCAGC CTCAACAGCA GCAGCCCCAG 
CTGAAGAAAC CCCGGGTGGT TCTGGCTCCG GAAGAGAAGG AAGCTCTGAA GCGGGCCTAC 
CAGCAGAAGC CTTACCCCTC CCCTAAAACC ATTGAGGAGT TAGCGAGTCA GCTCAACCTC 
AAAACCAGCA CCGTCATCAA CTGGTTCCAC AACTACAGGT CTCGTATCCG GCGAGAGCTT 
TTTATCGAAG AGATCCAGGC AGCAGCAGGT GAAGGTGGAT CTCCATCTGC ACGGCCTGGA 
AAATCTGGAG GCGAGGGAGA AAACAGCTGC GACGGCACTG AGTCCGACAG CACGGCAGAA 
GGCCGACCGA GTGGCTCAGA GGAGCGCAAG GGCTTCCTGG AGAGCCCGGG CCTCGGCCAC 
AGAGAAGAAG ATGGCGAGGG CGAGTTTCCT AGTGCCTCGG GCACCAGCCG GCCACGAGGA 
GGCATCATAG ACAGCCTGTT CGGCATCCCC GAGTCCATAT CCGTCACCAA TGCTGCCACG 
ACCCCCTGCC GAAATCCAAA GGATAGCAGT CTACGCAAGA AGAAAGCAGC CAACCTCAAC 
AACATCATCC ACCGGCTGGA GAAAGCGGCG AACCGAGACG AGCCTCACGA ATGGGAGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172776.1
CDS	182..4624
Translation	MAANAGSMFQYWKRFDLQQLQKELDATATALANRQDESEQSRKKLIDQSREFKKNTPEDLRKQIAPLLKGFQAEIDALSKRSKESEAAFLSVYKRLIDVPDPVSALEAAQQLQVTVRKMHDLETENQRLKDTLQEYEREISEVKNQEVTIKALKEKLEQYERSLQAKNTEEAVEPNLDSSEREERQSKGENLPNNYADKESQPEQSQESRTEDSDVKTQSLQTALEATQAELHELKTKYDEESTAKANEIEAVMTDLERANQRAETAEREAGTLREQLTSAGKTMQSSNQIQNAPEMDKSLEAELGAKEREVERLAEDVQRLQASLAQLSETSTNQISQLEQQLSSKEALLKQLEEKLQDQSDYEEIKRELCTLKSVEHNSSDHHSSAQDSSKPLEMLLTGKDHSQLSESALKRLANSDFTGAVGRKLKETTVENPQRLPATPVPTTLQGSPPPPSPQLLMRTGTATSESAASANGTHPFSPTGIGPDFFTPGMASVGATFPPLAGKMALNSLLQRQLMQTIYTKALQDPSPSSVLFGPPHYATTNSLSSPLPPQSAGSPEVNGMAPSPSQSEGAGSTSEGEEMDTAEIARQVKEQLIKHNIGQRVFGHYVLGLSQGSVSEILARPKPWSKLTIRGKEPFHKMKHFLSDEQNILALRSIQGRQRENPGQNLNRVFQDVPKRRGGSEGSTRPGNITTRIRTPETGSDEAIKSILEQAKRELQVQKADGVGERHRRSGYHRGSGSYFQIYAHSLRLVESSQPPSSSSNSDENIRSILEQARREMEAQQVALEPVLKASSMGPTDLSLLGSKLLGSPLSTLPYTPITQSLKKPSSSPLLDFHHGGVKKEMGEVAVSDVGLDGVVPKLGRLSESGLGGGSWREHWWSTGLSERRGSSQPSVSEDAHSQEDSKEKLPRLGSTAPVAPASSLDYWKDWPSSESPYSQSSELSLQMPSGSETPQSSPLPSSSPLLSLPKVAKPVVPPLTPEQYEYYMYQEVDTVDLTQQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKHWSKLTQKGREPFIRMQLWLNGELGQALMPMPGQTQEITPKTSASCSPAPESPLSSAEESVKSQQEEQMCRSSVQEPSEASESQPGTPLPLPLPGQAGLSIQEMVAMSAELDTYVITKKVKEVLTDNNLGQRLFGESILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLSDPRNVEKLMDMKRMEKKAYMKRRHSSIGESHSLESGLSAADLTQGASPQHLSQQQPPQQMQPQQQQPQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEELASQLNLKTSTVINWFHNYRSRIRRELFIEEIQAAAGEGGSPSARPGKSGGEGENSCDGTESDSTAEGRPSGSEERKGFLESPGLGHREEDGEGEFPSASGTSRPRGGIIDSLFGIPESISVTNAATTPCRNPKDSSLRKKKAANLNNIIHRLEKAANRDEPHEWEF

Target ORF information:

RefSeq Version	XM_005172719.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172719.3

ORF Insert Sequence:

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGAGAACCC AGGCCAGAAC CTGAACAGAG TGTTTCAGGA TGTTCCGAAG 
CGAAGAGGCG GCTCTGAAGG CTCCACGCGG CCAGGTAACA TCACCACACG GATCCGCACG 
CCTGAAACCG GCTCCGATGA AGCCATCAAG TCCATCTTAG AGCAGGCCAA GAGAGAGTTA 
CAAGTGCAGA AAGCAGACGG CGTGGGGGAG AGGCATCGGC GATCAGGTTA CCACAGAGGG 
TCGGGCAGCT ATTTCCAAAT ATATGCACAC AGTCTAAGAC TTGTCGAGAG CTCTCAGCCT 
CCGTCCTCCT CCAGCAACTC GGACGAGAAC ATTCGCTCCA TCCTCGAGCA GGCTCGCAGG 
GAGATGGAGG CGCAGCAGGT GGCACTGGAG CCCGTCTTGA AGGCCAGCTC CATGGGCCCG 
ACGGACCTCT CGCTCCTGGG CTCCAAACTC CTGGGCTCCC CGCTCTCCAC CCTGCCCTAC 
ACCCCCATCA CCCAGTCCCT GAAGAAGCCC TCTAGTTCAC CGCTGCTGGA CTTCCACCAT 
GGTGGAGTGA AGAAGGAGAT GGGCGAGGTG GCCGTTTCGG ATGTGGGTCT GGACGGAGTG 
GTGCCCAAGC TGGGCCGTTT GTCTGAGAGC GGTTTGGGTG GTGGCTCGTG GAGGGAGCAC 
TGGTGGAGCA CAGGCCTGTC AGAGCGCCGC GGGTCCAGCC AGCCCTCCGT TAGTGAAGAC 
GCACACAGTC AAGAGGACAG CAAAGAGAAG CTCCCTCGGC TGGGCAGCAC TGCGCCGGTG 
GCTCCTGCGT CGTCTCTGGA TTACTGGAAG GACTGGCCGA GCTCAGAATC GCCGTACTCT 
CAGAGCTCAG AGCTCAGCCT GCAGATGCCC AGTGGCAGTG AGACCCCTCA GAGCAGCCCA 
CTGCCTTCCT CCTCGCCCTT ACTGTCCCTG CCCAAAGTGG CCAAACCTGT GGTGCCGCCG 
CTTACGCCAG AGCAATACGA GTACTACATG TACCAGGAGG TGGACACTGT CGACCTCACA 
CAGCAGGTCA AAGAAAAACT CGCCAAGAAC GGCATCTGCC AGCGCATCTT CGGGGAGAAG 
GTGCTGGGTC TCTCTCAGGG GAGTGTGAGC GACATGCTCT CTCGACCAAA GCACTGGAGC 
AAACTCACAC AGAAGGGAAG GGAGCCGTTT ATACGCATGC AGTTATGGCT GAACGGAGAG 
CTGGGACAGG CACTGATGCC AATGCCTGGA CAGACACAAG AAATCACCCC CAAAACCTCG 
GCCAGCTGCA GTCCTGCCCC AGAATCCCCC CTGAGCTCGG CTGAGGAGTC TGTGAAGAGC 
CAGCAGGAGG AGCAGATGTG CCGATCCTCC GTCCAGGAGC CCAGCGAAGC CTCCGAGTCT 
CAGCCCGGGA CGCCGCTGCC GCTGCCTCTG CCGGGACAGG CCGGTCTCAG CATTCAGGAG 
ATGGTGGCCA TGTCAGCCGA GCTGGACACG TACGTCATCA CGAAAAAAGT CAAAGAAGTC 
CTCACTGATA ACAACCTGGG CCAGCGTCTG TTCGGGGAGA GCATTCTGGG TTTGACGCAG 
GGCTCAGTGT CTGATCTGCT GGCTCGACCC AAACCCTGGC ACAAGCTCAG CCTGAAGGGT 
CGCGAGCCGT TCGTGCGCAT GCAGCTGTGG TTGAGCGACC CGCGCAACGT GGAGAAGCTG 
ATGGACATGA AGCGCATGGA GAAGAAAGCC TACATGAAAA GGCGCCACAG CTCCATCGGT 
GAGAGTCATT CGCTGGAGAG CGGCTTGTCT GCAGCAGACC TGACACAGGG TGCCAGTCCC 
CAACACCTAT CCCAGCAGCA GCCGCCGCAG CAGATGCAGC CTCAACAGCA GCAGCCCCAG 
CTGAAGAAAC CCCGGGTGGT TCTGGCTCCG GAAGAGAAGG AAGCTCTGAA GCGGGCCTAC 
CAGCAGAAGC CTTACCCCTC CCCTAAAACC ATTGAGGAGT TAGCGAGTCA GCTCAACCTC 
AAAACCAGCA CCGTCATCAA CTGGTTCCAC AACTACAGGT CTCGTATCCG GCGAGAGCTT 
TTTATCGAAG AGATCCAGGC AGCAGCAGGT GAAGGTGGAT CTCCATCTGC ACGGCCTGGA 
AAATCTGGAG GCGAGGGAGA AAACAGCTGC GACGGCACTG AGTCCGACAG CACGGCAGAA 
GGCCGACCGA GTGGCTCAGA GGAGCGCAAG GGCTTCCTGG AGAGCCCGGG CCTCGGCCAC 
AGAGAAGAAG ATGGCGAGGG CGAGTTTCCT AGTGCCTCGG GCACCAGCCG GCCACGAGGA 
GGCATCATAG ACAGCCTGTT CGGCATCCCC GAGTCCATAT CCGTCACCAA TGCTGCCACG 
ACCCCCTGCC GAAATCCAAA GGATAGCAGT CTACGCAAGA AGAAAGCAGC CAACCTCAAC 
AACATCATCC ACCGGCTGGA GAAAGCGGCG AACCGAGACG AGCCTCACGA ATGGGAGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cux1a ( XM_005172720.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172777.1 Danio rerio cut-like homeobox 1a (cux1a), transcript variant X3, mRNA.

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CloneID	ODa16298
Clone ID Related Accession (Same CDS sequence)	XM_005172720.4 , XM_005172720.3
Accession Version	XM_005172720.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	homeobox protein cut-like 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172720.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGGTAACAT CACCACACGG ATCCGCACGC CTGAAACCGG CTCCGATGAA 
GCCATCAAGT CCATCTTAGA GCAGGCCAAG AGAGAGTTAC AAGTGCAGAA AGCAGACGGC 
GTGGGGGAGA GGCATCGGCG ATCAGGTTAC CACAGAGGGT CGGGCAGCTA TTTCCAAATA 
TATGCACACA GTCTAAGACT TGAAGAATCT AACATAGATC TGAAACCCAT GAGGATGACT 
AAGCAATTCG AGAGCTCTCA GCCTCCGTCC TCCTCCAGCA ACTCGGACGA GAACATTCGC 
TCCATCCTCG AGCAGGCTCG CAGGGAGATG GAGGCGCAGC AGGTGGCACT GGAGCCCGTC 
TTGAAGGCCA GCTCCATGGG CCCGACGGAC CTCTCGCTCC TGGGCTCCAA ACTCCTGGGC 
TCCCCGCTCT CCACCCTGCC CTACACCCCC ATCACCCAGT CCCTGAAGAA GCCCTCTAGT 
TCACCGCTGC TGGACTTCCA CCATGGTGGA GTGAAGAAGG AGATGGGCGA GGTGGCCGTT 
TCGGATGTGG GTCTGGACGG AGTGGTGCCC AAGCTGGGCC GTTTGTCTGA GAGCGGTTTG 
GGTGGTGGCT CGTGGAGGGA GCACTGGTGG AGCACAGGCC TGTCAGAGCG CCGCGGGTCC 
AGCCAGCCCT CCGTTAGTGA AGACGCACAC AGTCAAGAGG ACAGCAAAGA GAAGCTCCCT 
CGGCTGGGCA GCACTGCGCC GGTGGCTCCT GCGTCGTCTC TGGATTACTG GAAGGACTGG 
CCGAGCTCAG AATCGCCGTA CTCTCAGAGC TCAGAGCTCA GCCTGCAGAT GCCCAGTGGC 
AGTGAGACCC CTCAGAGCAG CCCACTGCCT TCCTCCTCGC CCTTACTGTC CCTGCCCAAA 
GTGGCCAAAC CTGTGGTGCC GCCGCTTACG CCAGAGCAAT ACGAGTACTA CATGTACCAG 
GAGGTGGACA CTGTCGACCT CACACAGCAG GTCAAAGAAA AACTCGCCAA GAACGGCATC 
TGCCAGCGCA TCTTCGGGGA GAAGGTGCTG GGTCTCTCTC AGGGGAGTGT GAGCGACATG 
CTCTCTCGAC CAAAGCACTG GAGCAAACTC ACACAGAAGG GAAGGGAGCC GTTTATACGC 
ATGCAGTTAT GGCTGAACGG AGAGCTGGGA CAGGCACTGA TGCCAATGCC TGGACAGACA 
CAAGAAATCA CCCCCAAAAC CTCGGCCAGC TGCAGTCCTG CCCCAGAATC CCCCCTGAGC 
TCGGCTGAGG AGTCTGTGAA GAGCCAGCAG GAGGAGCAGA TGTGCCGATC CTCCGTCCAG 
GAGCCCAGCG AAGCCTCCGA GTCTCAGCCC GGGACGCCGC TGCCGCTGCC TCTGCCGGGA 
CAGGCCGGTC TCAGCATTCA GGAGATGGTG GCCATGTCAG CCGAGCTGGA CACGTACGTC 
ATCACGAAAA AAGTCAAAGA AGTCCTCACT GATAACAACC TGGGCCAGCG TCTGTTCGGG 
GAGAGCATTC TGGGTTTGAC GCAGGGCTCA GTGTCTGATC TGCTGGCTCG ACCCAAACCC 
TGGCACAAGC TCAGCCTGAA GGGTCGCGAG CCGTTCGTGC GCATGCAGCT GTGGTTGAGC 
GACCCGCGCA ACGTGGAGAA GCTGATGGAC ATGAAGCGCA TGGAGAAGAA AGCCTACATG 
AAAAGGCGCC ACAGCTCCAT CGGTGAGAGT CATTCGCTGG AGAGCGGCTT GTCTGCAGCA 
GACCTGACAC AGGGTGCCAG TCCCCAACAC CTATCCCAGC AGCAGCCGCC GCAGCAGATG 
CAGCCTCAAC AGCAGCAGCC CCAGCTGAAG AAACCCCGGG TGGTTCTGGC TCCGGAAGAG 
AAGGAAGCTC TGAAGCGGGC CTACCAGCAG AAGCCTTACC CCTCCCCTAA AACCATTGAG 
GAGTTAGCGA GTCAGCTCAA CCTCAAAACC AGCACCGTCA TCAACTGGTT CCACAACTAC 
AGGTCTCGTA TCCGGCGAGA GCTTTTTATC GAAGAGATCC AGGCAGCAGC AGGTGAAGGT 
GGATCTCCAT CTGCACGGCC TGGAAAATCT GGAGGCGAGG GAGAAAACAG CTGCGACGGC 
ACTGAGTCCG ACAGCACGGC AGAAGGCCGA CCGAGTGGCT CAGAGGAGCG CAAGGGCTTC 
CTGGAGAGCC CGGGCCTCGG CCACAGAGAA GAAGATGGCG AGGGCGAGTT TCCTAGTGCC 
TCGGGCACCA GCCGGCCACG AGGAGGCATC ATAGACAGCC TGTTCGGCAT CCCCGAGTCC 
ATATCCGTCA CCAATGCTGC CACGACCCCC TGCCGAAATC CAAAGGATAG CAGTCTACGC 
AAGAAGAAAG CAGCCAACCT CAACAACATC ATCCACCGGC TGGAGAAAGC GGCGAACCGA 
GACGAGCCTC ACGAATGGGA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172777.1
CDS	79..4485
Translation	MAANAGSMFQYWKRFDLQQLQKELDATATALANRQDESEQSRKKLIDQSREFKKNTPEDLRKQIAPLLKGFQAEIDALSKRSKESEAAFLSVYKRLIDVPDPVSALEAAQQLQVTVRKMHDLETENQRLKDTLQEYEREISEVKNQEVTIKALKEKLEQYERSLQAKNTEEAVEPNLDSSEREERQSKGENLPNNYADKESQPEQSQESRTEDSDVKTQSLQTALEATQAELHELKTKYDEESTAKANEIEAVMTDLERANQRAETAEREAGTLREQLTSAGKTMQSSNQIQNAPEMDKSLEAELGAKEREVERLAEDVQRLQASLAQLSETSTNQISQLEQQLSSKEALLKQLEEKLQDQSDYEEIKRELCTLKSVEHNSSDHHSSAQDSSKPLEMLLTGKDHSQLSESALKRLANSDFTGAVGRKLKETTVENPQRLPATPVPTTLQGSPPPPSPQLLMRTGTATSESAASANGTHPFSPTGIGPDFFTPGMASVGATFPPLAGKMALNSLLQRQLMQTIYTKALQDPSPSSVLFGPPHYATTNSLSSPLPPQSAGSPEVNGMAPSPSQSEGAGSTSEGEEMDTAEIARQVKEQLIKHNIGQRVFGHYVLGLSQGSVSEILARPKPWSKLTIRGKEPFHKMKHFLSDEQNILALRSIQGRQRGNITTRIRTPETGSDEAIKSILEQAKRELQVQKADGVGERHRRSGYHRGSGSYFQIYAHSLRLEESNIDLKPMRMTKQFESSQPPSSSSNSDENIRSILEQARREMEAQQVALEPVLKASSMGPTDLSLLGSKLLGSPLSTLPYTPITQSLKKPSSSPLLDFHHGGVKKEMGEVAVSDVGLDGVVPKLGRLSESGLGGGSWREHWWSTGLSERRGSSQPSVSEDAHSQEDSKEKLPRLGSTAPVAPASSLDYWKDWPSSESPYSQSSELSLQMPSGSETPQSSPLPSSSPLLSLPKVAKPVVPPLTPEQYEYYMYQEVDTVDLTQQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKHWSKLTQKGREPFIRMQLWLNGELGQALMPMPGQTQEITPKTSASCSPAPESPLSSAEESVKSQQEEQMCRSSVQEPSEASESQPGTPLPLPLPGQAGLSIQEMVAMSAELDTYVITKKVKEVLTDNNLGQRLFGESILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLSDPRNVEKLMDMKRMEKKAYMKRRHSSIGESHSLESGLSAADLTQGASPQHLSQQQPPQQMQPQQQQPQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEELASQLNLKTSTVINWFHNYRSRIRRELFIEEIQAAAGEGGSPSARPGKSGGEGENSCDGTESDSTAEGRPSGSEERKGFLESPGLGHREEDGEGEFPSASGTSRPRGGIIDSLFGIPESISVTNAATTPCRNPKDSSLRKKKAANLNNIIHRLEKAANRDEPHEWEF

Target ORF information:

RefSeq Version	XM_005172720.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172720.4

ORF Insert Sequence:

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGGTAACAT CACCACACGG ATCCGCACGC CTGAAACCGG CTCCGATGAA 
GCCATCAAGT CCATCTTAGA GCAGGCCAAG AGAGAGTTAC AAGTGCAGAA AGCAGACGGC 
GTGGGGGAGA GGCATCGGCG ATCAGGTTAC CACAGAGGGT CGGGCAGCTA TTTCCAAATA 
TATGCACACA GTCTAAGACT TGAAGAATCT AACATAGATC TGAAACCCAT GAGGATGACT 
AAGCAATTCG AGAGCTCTCA GCCTCCGTCC TCCTCCAGCA ACTCGGACGA GAACATTCGC 
TCCATCCTCG AGCAGGCTCG CAGGGAGATG GAGGCGCAGC AGGTGGCACT GGAGCCCGTC 
TTGAAGGCCA GCTCCATGGG CCCGACGGAC CTCTCGCTCC TGGGCTCCAA ACTCCTGGGC 
TCCCCGCTCT CCACCCTGCC CTACACCCCC ATCACCCAGT CCCTGAAGAA GCCCTCTAGT 
TCACCGCTGC TGGACTTCCA CCATGGTGGA GTGAAGAAGG AGATGGGCGA GGTGGCCGTT 
TCGGATGTGG GTCTGGACGG AGTGGTGCCC AAGCTGGGCC GTTTGTCTGA GAGCGGTTTG 
GGTGGTGGCT CGTGGAGGGA GCACTGGTGG AGCACAGGCC TGTCAGAGCG CCGCGGGTCC 
AGCCAGCCCT CCGTTAGTGA AGACGCACAC AGTCAAGAGG ACAGCAAAGA GAAGCTCCCT 
CGGCTGGGCA GCACTGCGCC GGTGGCTCCT GCGTCGTCTC TGGATTACTG GAAGGACTGG 
CCGAGCTCAG AATCGCCGTA CTCTCAGAGC TCAGAGCTCA GCCTGCAGAT GCCCAGTGGC 
AGTGAGACCC CTCAGAGCAG CCCACTGCCT TCCTCCTCGC CCTTACTGTC CCTGCCCAAA 
GTGGCCAAAC CTGTGGTGCC GCCGCTTACG CCAGAGCAAT ACGAGTACTA CATGTACCAG 
GAGGTGGACA CTGTCGACCT CACACAGCAG GTCAAAGAAA AACTCGCCAA GAACGGCATC 
TGCCAGCGCA TCTTCGGGGA GAAGGTGCTG GGTCTCTCTC AGGGGAGTGT GAGCGACATG 
CTCTCTCGAC CAAAGCACTG GAGCAAACTC ACACAGAAGG GAAGGGAGCC GTTTATACGC 
ATGCAGTTAT GGCTGAACGG AGAGCTGGGA CAGGCACTGA TGCCAATGCC TGGACAGACA 
CAAGAAATCA CCCCCAAAAC CTCGGCCAGC TGCAGTCCTG CCCCAGAATC CCCCCTGAGC 
TCGGCTGAGG AGTCTGTGAA GAGCCAGCAG GAGGAGCAGA TGTGCCGATC CTCCGTCCAG 
GAGCCCAGCG AAGCCTCCGA GTCTCAGCCC GGGACGCCGC TGCCGCTGCC TCTGCCGGGA 
CAGGCCGGTC TCAGCATTCA GGAGATGGTG GCCATGTCAG CCGAGCTGGA CACGTACGTC 
ATCACGAAAA AAGTCAAAGA AGTCCTCACT GATAACAACC TGGGCCAGCG TCTGTTCGGG 
GAGAGCATTC TGGGTTTGAC GCAGGGCTCA GTGTCTGATC TGCTGGCTCG ACCCAAACCC 
TGGCACAAGC TCAGCCTGAA GGGTCGCGAG CCGTTCGTGC GCATGCAGCT GTGGTTGAGC 
GACCCGCGCA ACGTGGAGAA GCTGATGGAC ATGAAGCGCA TGGAGAAGAA AGCCTACATG 
AAAAGGCGCC ACAGCTCCAT CGGTGAGAGT CATTCGCTGG AGAGCGGCTT GTCTGCAGCA 
GACCTGACAC AGGGTGCCAG TCCCCAACAC CTATCCCAGC AGCAGCCGCC GCAGCAGATG 
CAGCCTCAAC AGCAGCAGCC CCAGCTGAAG AAACCCCGGG TGGTTCTGGC TCCGGAAGAG 
AAGGAAGCTC TGAAGCGGGC CTACCAGCAG AAGCCTTACC CCTCCCCTAA AACCATTGAG 
GAGTTAGCGA GTCAGCTCAA CCTCAAAACC AGCACCGTCA TCAACTGGTT CCACAACTAC 
AGGTCTCGTA TCCGGCGAGA GCTTTTTATC GAAGAGATCC AGGCAGCAGC AGGTGAAGGT 
GGATCTCCAT CTGCACGGCC TGGAAAATCT GGAGGCGAGG GAGAAAACAG CTGCGACGGC 
ACTGAGTCCG ACAGCACGGC AGAAGGCCGA CCGAGTGGCT CAGAGGAGCG CAAGGGCTTC 
CTGGAGAGCC CGGGCCTCGG CCACAGAGAA GAAGATGGCG AGGGCGAGTT TCCTAGTGCC 
TCGGGCACCA GCCGGCCACG AGGAGGCATC ATAGACAGCC TGTTCGGCAT CCCCGAGTCC 
ATATCCGTCA CCAATGCTGC CACGACCCCC TGCCGAAATC CAAAGGATAG CAGTCTACGC 
AAGAAGAAAG CAGCCAACCT CAACAACATC ATCCACCGGC TGGAGAAAGC GGCGAACCGA 
GACGAGCCTC ACGAATGGGA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cux1a ( XM_005172720.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172777.1 Danio rerio cut-like homeobox 1a (cux1a), transcript variant X3, mRNA.

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CloneID	ODa16298
Clone ID Related Accession (Same CDS sequence)	XM_005172720.4 , XM_005172720.3
Accession Version	XM_005172720.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	homeobox protein cut-like 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172720.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGGTAACAT CACCACACGG ATCCGCACGC CTGAAACCGG CTCCGATGAA 
GCCATCAAGT CCATCTTAGA GCAGGCCAAG AGAGAGTTAC AAGTGCAGAA AGCAGACGGC 
GTGGGGGAGA GGCATCGGCG ATCAGGTTAC CACAGAGGGT CGGGCAGCTA TTTCCAAATA 
TATGCACACA GTCTAAGACT TGAAGAATCT AACATAGATC TGAAACCCAT GAGGATGACT 
AAGCAATTCG AGAGCTCTCA GCCTCCGTCC TCCTCCAGCA ACTCGGACGA GAACATTCGC 
TCCATCCTCG AGCAGGCTCG CAGGGAGATG GAGGCGCAGC AGGTGGCACT GGAGCCCGTC 
TTGAAGGCCA GCTCCATGGG CCCGACGGAC CTCTCGCTCC TGGGCTCCAA ACTCCTGGGC 
TCCCCGCTCT CCACCCTGCC CTACACCCCC ATCACCCAGT CCCTGAAGAA GCCCTCTAGT 
TCACCGCTGC TGGACTTCCA CCATGGTGGA GTGAAGAAGG AGATGGGCGA GGTGGCCGTT 
TCGGATGTGG GTCTGGACGG AGTGGTGCCC AAGCTGGGCC GTTTGTCTGA GAGCGGTTTG 
GGTGGTGGCT CGTGGAGGGA GCACTGGTGG AGCACAGGCC TGTCAGAGCG CCGCGGGTCC 
AGCCAGCCCT CCGTTAGTGA AGACGCACAC AGTCAAGAGG ACAGCAAAGA GAAGCTCCCT 
CGGCTGGGCA GCACTGCGCC GGTGGCTCCT GCGTCGTCTC TGGATTACTG GAAGGACTGG 
CCGAGCTCAG AATCGCCGTA CTCTCAGAGC TCAGAGCTCA GCCTGCAGAT GCCCAGTGGC 
AGTGAGACCC CTCAGAGCAG CCCACTGCCT TCCTCCTCGC CCTTACTGTC CCTGCCCAAA 
GTGGCCAAAC CTGTGGTGCC GCCGCTTACG CCAGAGCAAT ACGAGTACTA CATGTACCAG 
GAGGTGGACA CTGTCGACCT CACACAGCAG GTCAAAGAAA AACTCGCCAA GAACGGCATC 
TGCCAGCGCA TCTTCGGGGA GAAGGTGCTG GGTCTCTCTC AGGGGAGTGT GAGCGACATG 
CTCTCTCGAC CAAAGCACTG GAGCAAACTC ACACAGAAGG GAAGGGAGCC GTTTATACGC 
ATGCAGTTAT GGCTGAACGG AGAGCTGGGA CAGGCACTGA TGCCAATGCC TGGACAGACA 
CAAGAAATCA CCCCCAAAAC CTCGGCCAGC TGCAGTCCTG CCCCAGAATC CCCCCTGAGC 
TCGGCTGAGG AGTCTGTGAA GAGCCAGCAG GAGGAGCAGA TGTGCCGATC CTCCGTCCAG 
GAGCCCAGCG AAGCCTCCGA GTCTCAGCCC GGGACGCCGC TGCCGCTGCC TCTGCCGGGA 
CAGGCCGGTC TCAGCATTCA GGAGATGGTG GCCATGTCAG CCGAGCTGGA CACGTACGTC 
ATCACGAAAA AAGTCAAAGA AGTCCTCACT GATAACAACC TGGGCCAGCG TCTGTTCGGG 
GAGAGCATTC TGGGTTTGAC GCAGGGCTCA GTGTCTGATC TGCTGGCTCG ACCCAAACCC 
TGGCACAAGC TCAGCCTGAA GGGTCGCGAG CCGTTCGTGC GCATGCAGCT GTGGTTGAGC 
GACCCGCGCA ACGTGGAGAA GCTGATGGAC ATGAAGCGCA TGGAGAAGAA AGCCTACATG 
AAAAGGCGCC ACAGCTCCAT CGGTGAGAGT CATTCGCTGG AGAGCGGCTT GTCTGCAGCA 
GACCTGACAC AGGGTGCCAG TCCCCAACAC CTATCCCAGC AGCAGCCGCC GCAGCAGATG 
CAGCCTCAAC AGCAGCAGCC CCAGCTGAAG AAACCCCGGG TGGTTCTGGC TCCGGAAGAG 
AAGGAAGCTC TGAAGCGGGC CTACCAGCAG AAGCCTTACC CCTCCCCTAA AACCATTGAG 
GAGTTAGCGA GTCAGCTCAA CCTCAAAACC AGCACCGTCA TCAACTGGTT CCACAACTAC 
AGGTCTCGTA TCCGGCGAGA GCTTTTTATC GAAGAGATCC AGGCAGCAGC AGGTGAAGGT 
GGATCTCCAT CTGCACGGCC TGGAAAATCT GGAGGCGAGG GAGAAAACAG CTGCGACGGC 
ACTGAGTCCG ACAGCACGGC AGAAGGCCGA CCGAGTGGCT CAGAGGAGCG CAAGGGCTTC 
CTGGAGAGCC CGGGCCTCGG CCACAGAGAA GAAGATGGCG AGGGCGAGTT TCCTAGTGCC 
TCGGGCACCA GCCGGCCACG AGGAGGCATC ATAGACAGCC TGTTCGGCAT CCCCGAGTCC 
ATATCCGTCA CCAATGCTGC CACGACCCCC TGCCGAAATC CAAAGGATAG CAGTCTACGC 
AAGAAGAAAG CAGCCAACCT CAACAACATC ATCCACCGGC TGGAGAAAGC GGCGAACCGA 
GACGAGCCTC ACGAATGGGA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172777.1
CDS	183..4589
Translation	MAANAGSMFQYWKRFDLQQLQKELDATATALANRQDESEQSRKKLIDQSREFKKNTPEDLRKQIAPLLKGFQAEIDALSKRSKESEAAFLSVYKRLIDVPDPVSALEAAQQLQVTVRKMHDLETENQRLKDTLQEYEREISEVKNQEVTIKALKEKLEQYERSLQAKNTEEAVEPNLDSSEREERQSKGENLPNNYADKESQPEQSQESRTEDSDVKTQSLQTALEATQAELHELKTKYDEESTAKANEIEAVMTDLERANQRAETAEREAGTLREQLTSAGKTMQSSNQIQNAPEMDKSLEAELGAKEREVERLAEDVQRLQASLAQLSETSTNQISQLEQQLSSKEALLKQLEEKLQDQSDYEEIKRELCTLKSVEHNSSDHHSSAQDSSKPLEMLLTGKDHSQLSESALKRLANSDFTGAVGRKLKETTVENPQRLPATPVPTTLQGSPPPPSPQLLMRTGTATSESAASANGTHPFSPTGIGPDFFTPGMASVGATFPPLAGKMALNSLLQRQLMQTIYTKALQDPSPSSVLFGPPHYATTNSLSSPLPPQSAGSPEVNGMAPSPSQSEGAGSTSEGEEMDTAEIARQVKEQLIKHNIGQRVFGHYVLGLSQGSVSEILARPKPWSKLTIRGKEPFHKMKHFLSDEQNILALRSIQGRQRGNITTRIRTPETGSDEAIKSILEQAKRELQVQKADGVGERHRRSGYHRGSGSYFQIYAHSLRLEESNIDLKPMRMTKQFESSQPPSSSSNSDENIRSILEQARREMEAQQVALEPVLKASSMGPTDLSLLGSKLLGSPLSTLPYTPITQSLKKPSSSPLLDFHHGGVKKEMGEVAVSDVGLDGVVPKLGRLSESGLGGGSWREHWWSTGLSERRGSSQPSVSEDAHSQEDSKEKLPRLGSTAPVAPASSLDYWKDWPSSESPYSQSSELSLQMPSGSETPQSSPLPSSSPLLSLPKVAKPVVPPLTPEQYEYYMYQEVDTVDLTQQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKHWSKLTQKGREPFIRMQLWLNGELGQALMPMPGQTQEITPKTSASCSPAPESPLSSAEESVKSQQEEQMCRSSVQEPSEASESQPGTPLPLPLPGQAGLSIQEMVAMSAELDTYVITKKVKEVLTDNNLGQRLFGESILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLSDPRNVEKLMDMKRMEKKAYMKRRHSSIGESHSLESGLSAADLTQGASPQHLSQQQPPQQMQPQQQQPQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEELASQLNLKTSTVINWFHNYRSRIRRELFIEEIQAAAGEGGSPSARPGKSGGEGENSCDGTESDSTAEGRPSGSEERKGFLESPGLGHREEDGEGEFPSASGTSRPRGGIIDSLFGIPESISVTNAATTPCRNPKDSSLRKKKAANLNNIIHRLEKAANRDEPHEWEF

Target ORF information:

RefSeq Version	XM_005172720.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172720.3

ORF Insert Sequence:

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGGTAACAT CACCACACGG ATCCGCACGC CTGAAACCGG CTCCGATGAA 
GCCATCAAGT CCATCTTAGA GCAGGCCAAG AGAGAGTTAC AAGTGCAGAA AGCAGACGGC 
GTGGGGGAGA GGCATCGGCG ATCAGGTTAC CACAGAGGGT CGGGCAGCTA TTTCCAAATA 
TATGCACACA GTCTAAGACT TGAAGAATCT AACATAGATC TGAAACCCAT GAGGATGACT 
AAGCAATTCG AGAGCTCTCA GCCTCCGTCC TCCTCCAGCA ACTCGGACGA GAACATTCGC 
TCCATCCTCG AGCAGGCTCG CAGGGAGATG GAGGCGCAGC AGGTGGCACT GGAGCCCGTC 
TTGAAGGCCA GCTCCATGGG CCCGACGGAC CTCTCGCTCC TGGGCTCCAA ACTCCTGGGC 
TCCCCGCTCT CCACCCTGCC CTACACCCCC ATCACCCAGT CCCTGAAGAA GCCCTCTAGT 
TCACCGCTGC TGGACTTCCA CCATGGTGGA GTGAAGAAGG AGATGGGCGA GGTGGCCGTT 
TCGGATGTGG GTCTGGACGG AGTGGTGCCC AAGCTGGGCC GTTTGTCTGA GAGCGGTTTG 
GGTGGTGGCT CGTGGAGGGA GCACTGGTGG AGCACAGGCC TGTCAGAGCG CCGCGGGTCC 
AGCCAGCCCT CCGTTAGTGA AGACGCACAC AGTCAAGAGG ACAGCAAAGA GAAGCTCCCT 
CGGCTGGGCA GCACTGCGCC GGTGGCTCCT GCGTCGTCTC TGGATTACTG GAAGGACTGG 
CCGAGCTCAG AATCGCCGTA CTCTCAGAGC TCAGAGCTCA GCCTGCAGAT GCCCAGTGGC 
AGTGAGACCC CTCAGAGCAG CCCACTGCCT TCCTCCTCGC CCTTACTGTC CCTGCCCAAA 
GTGGCCAAAC CTGTGGTGCC GCCGCTTACG CCAGAGCAAT ACGAGTACTA CATGTACCAG 
GAGGTGGACA CTGTCGACCT CACACAGCAG GTCAAAGAAA AACTCGCCAA GAACGGCATC 
TGCCAGCGCA TCTTCGGGGA GAAGGTGCTG GGTCTCTCTC AGGGGAGTGT GAGCGACATG 
CTCTCTCGAC CAAAGCACTG GAGCAAACTC ACACAGAAGG GAAGGGAGCC GTTTATACGC 
ATGCAGTTAT GGCTGAACGG AGAGCTGGGA CAGGCACTGA TGCCAATGCC TGGACAGACA 
CAAGAAATCA CCCCCAAAAC CTCGGCCAGC TGCAGTCCTG CCCCAGAATC CCCCCTGAGC 
TCGGCTGAGG AGTCTGTGAA GAGCCAGCAG GAGGAGCAGA TGTGCCGATC CTCCGTCCAG 
GAGCCCAGCG AAGCCTCCGA GTCTCAGCCC GGGACGCCGC TGCCGCTGCC TCTGCCGGGA 
CAGGCCGGTC TCAGCATTCA GGAGATGGTG GCCATGTCAG CCGAGCTGGA CACGTACGTC 
ATCACGAAAA AAGTCAAAGA AGTCCTCACT GATAACAACC TGGGCCAGCG TCTGTTCGGG 
GAGAGCATTC TGGGTTTGAC GCAGGGCTCA GTGTCTGATC TGCTGGCTCG ACCCAAACCC 
TGGCACAAGC TCAGCCTGAA GGGTCGCGAG CCGTTCGTGC GCATGCAGCT GTGGTTGAGC 
GACCCGCGCA ACGTGGAGAA GCTGATGGAC ATGAAGCGCA TGGAGAAGAA AGCCTACATG 
AAAAGGCGCC ACAGCTCCAT CGGTGAGAGT CATTCGCTGG AGAGCGGCTT GTCTGCAGCA 
GACCTGACAC AGGGTGCCAG TCCCCAACAC CTATCCCAGC AGCAGCCGCC GCAGCAGATG 
CAGCCTCAAC AGCAGCAGCC CCAGCTGAAG AAACCCCGGG TGGTTCTGGC TCCGGAAGAG 
AAGGAAGCTC TGAAGCGGGC CTACCAGCAG AAGCCTTACC CCTCCCCTAA AACCATTGAG 
GAGTTAGCGA GTCAGCTCAA CCTCAAAACC AGCACCGTCA TCAACTGGTT CCACAACTAC 
AGGTCTCGTA TCCGGCGAGA GCTTTTTATC GAAGAGATCC AGGCAGCAGC AGGTGAAGGT 
GGATCTCCAT CTGCACGGCC TGGAAAATCT GGAGGCGAGG GAGAAAACAG CTGCGACGGC 
ACTGAGTCCG ACAGCACGGC AGAAGGCCGA CCGAGTGGCT CAGAGGAGCG CAAGGGCTTC 
CTGGAGAGCC CGGGCCTCGG CCACAGAGAA GAAGATGGCG AGGGCGAGTT TCCTAGTGCC 
TCGGGCACCA GCCGGCCACG AGGAGGCATC ATAGACAGCC TGTTCGGCAT CCCCGAGTCC 
ATATCCGTCA CCAATGCTGC CACGACCCCC TGCCGAAATC CAAAGGATAG CAGTCTACGC 
AAGAAGAAAG CAGCCAACCT CAACAACATC ATCCACCGGC TGGAGAAAGC GGCGAACCGA 
GACGAGCCTC ACGAATGGGA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cux1a ( XM_005172721.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172778.1 Danio rerio cut-like homeobox 1a (cux1a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa16299
Clone ID Related Accession (Same CDS sequence)	XM_005172721.4 , XM_005172721.3
Accession Version	XM_005172721.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	homeobox protein cut-like 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172721.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGAGAACCC AGGCCAGAAC CTGAACAGAG TGTTTCAGGA TGTTCCGAAG 
CGAAGAGGCG GCTCTGAAGG CTCCACGCGG CCAGGTAACA TCACCACACG GATCCGCACG 
CCTGAAACCG GCTCCGATGA AGCCATCAAG TCCATCTTAG AGCAGGCCAA GAGAGAGTTA 
CAAGTGCAGA AAGCAGAAGA ATCTAACATA GATCTGAAAC CCATGAGGAT GACTAAGCAA 
TTCGAGAGCT CTCAGCCTCC GTCCTCCTCC AGCAACTCGG ACGAGAACAT TCGCTCCATC 
CTCGAGCAGG CTCGCAGGGA GATGGAGGCG CAGCAGGTGG CACTGGAGCC CGTCTTGAAG 
GCCAGCTCCA TGGGCCCGAC GGACCTCTCG CTCCTGGGCT CCAAACTCCT GGGCTCCCCG 
CTCTCCACCC TGCCCTACAC CCCCATCACC CAGTCCCTGA AGAAGCCCTC TAGTTCACCG 
CTGCTGGACT TCCACCATGG TGGAGTGAAG AAGGAGATGG GCGAGGTGGC CGTTTCGGAT 
GTGGGTCTGG ACGGAGTGGT GCCCAAGCTG GGCCGTTTGT CTGAGAGCGG TTTGGGTGGT 
GGCTCGTGGA GGGAGCACTG GTGGAGCACA GGCCTGTCAG AGCGCCGCGG GTCCAGCCAG 
CCCTCCGTTA GTGAAGACGC ACACAGTCAA GAGGACAGCA AAGAGAAGCT CCCTCGGCTG 
GGCAGCACTG CGCCGGTGGC TCCTGCGTCG TCTCTGGATT ACTGGAAGGA CTGGCCGAGC 
TCAGAATCGC CGTACTCTCA GAGCTCAGAG CTCAGCCTGC AGATGCCCAG TGGCAGTGAG 
ACCCCTCAGA GCAGCCCACT GCCTTCCTCC TCGCCCTTAC TGTCCCTGCC CAAAGTGGCC 
AAACCTGTGG TGCCGCCGCT TACGCCAGAG CAATACGAGT ACTACATGTA CCAGGAGGTG 
GACACTGTCG ACCTCACACA GCAGGTCAAA GAAAAACTCG CCAAGAACGG CATCTGCCAG 
CGCATCTTCG GGGAGAAGGT GCTGGGTCTC TCTCAGGGGA GTGTGAGCGA CATGCTCTCT 
CGACCAAAGC ACTGGAGCAA ACTCACACAG AAGGGAAGGG AGCCGTTTAT ACGCATGCAG 
TTATGGCTGA ACGGAGAGCT GGGACAGGCA CTGATGCCAA TGCCTGGACA GACACAAGAA 
ATCACCCCCA AAACCTCGGC CAGCTGCAGT CCTGCCCCAG AATCCCCCCT GAGCTCGGCT 
GAGGAGTCTG TGAAGAGCCA GCAGGAGGAG CAGATGTGCC GATCCTCCGT CCAGGAGCCC 
AGCGAAGCCT CCGAGTCTCA GCCCGGGACG CCGCTGCCGC TGCCTCTGCC GGGACAGGCC 
GGTCTCAGCA TTCAGGAGAT GGTGGCCATG TCAGCCGAGC TGGACACGTA CGTCATCACG 
AAAAAAGTCA AAGAAGTCCT CACTGATAAC AACCTGGGCC AGCGTCTGTT CGGGGAGAGC 
ATTCTGGGTT TGACGCAGGG CTCAGTGTCT GATCTGCTGG CTCGACCCAA ACCCTGGCAC 
AAGCTCAGCC TGAAGGGTCG CGAGCCGTTC GTGCGCATGC AGCTGTGGTT GAGCGACCCG 
CGCAACGTGG AGAAGCTGAT GGACATGAAG CGCATGGAGA AGAAAGCCTA CATGAAAAGG 
CGCCACAGCT CCATCGGTGA GAGTCATTCG CTGGAGAGCG GCTTGTCTGC AGCAGACCTG 
ACACAGGGTG CCAGTCCCCA ACACCTATCC CAGCAGCAGC CGCCGCAGCA GATGCAGCCT 
CAACAGCAGC AGCCCCAGCT GAAGAAACCC CGGGTGGTTC TGGCTCCGGA AGAGAAGGAA 
GCTCTGAAGC GGGCCTACCA GCAGAAGCCT TACCCCTCCC CTAAAACCAT TGAGGAGTTA 
GCGAGTCAGC TCAACCTCAA AACCAGCACC GTCATCAACT GGTTCCACAA CTACAGGTCT 
CGTATCCGGC GAGAGCTTTT TATCGAAGAG ATCCAGGCAG CAGCAGGTGA AGGTGGATCT 
CCATCTGCAC GGCCTGGAAA ATCTGGAGGC GAGGGAGAAA ACAGCTGCGA CGGCACTGAG 
TCCGACAGCA CGGCAGAAGG CCGACCGAGT GGCTCAGAGG AGCGCAAGGG CTTCCTGGAG 
AGCCCGGGCC TCGGCCACAG AGAAGAAGAT GGCGAGGGCG AGTTTCCTAG TGCCTCGGGC 
ACCAGCCGGC CACGAGGAGG CATCATAGAC AGCCTGTTCG GCATCCCCGA GTCCATATCC 
GTCACCAATG CTGCCACGAC CCCCTGCCGA AATCCAAAGG ATAGCAGTCT ACGCAAGAAG 
AAAGCAGCCA ACCTCAACAA CATCATCCAC CGGCTGGAGA AAGCGGCGAA CCGAGACGAG 
CCTCACGAAT GGGAGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172778.1
CDS	79..4479
Translation	MAANAGSMFQYWKRFDLQQLQKELDATATALANRQDESEQSRKKLIDQSREFKKNTPEDLRKQIAPLLKGFQAEIDALSKRSKESEAAFLSVYKRLIDVPDPVSALEAAQQLQVTVRKMHDLETENQRLKDTLQEYEREISEVKNQEVTIKALKEKLEQYERSLQAKNTEEAVEPNLDSSEREERQSKGENLPNNYADKESQPEQSQESRTEDSDVKTQSLQTALEATQAELHELKTKYDEESTAKANEIEAVMTDLERANQRAETAEREAGTLREQLTSAGKTMQSSNQIQNAPEMDKSLEAELGAKEREVERLAEDVQRLQASLAQLSETSTNQISQLEQQLSSKEALLKQLEEKLQDQSDYEEIKRELCTLKSVEHNSSDHHSSAQDSSKPLEMLLTGKDHSQLSESALKRLANSDFTGAVGRKLKETTVENPQRLPATPVPTTLQGSPPPPSPQLLMRTGTATSESAASANGTHPFSPTGIGPDFFTPGMASVGATFPPLAGKMALNSLLQRQLMQTIYTKALQDPSPSSVLFGPPHYATTNSLSSPLPPQSAGSPEVNGMAPSPSQSEGAGSTSEGEEMDTAEIARQVKEQLIKHNIGQRVFGHYVLGLSQGSVSEILARPKPWSKLTIRGKEPFHKMKHFLSDEQNILALRSIQGRQRENPGQNLNRVFQDVPKRRGGSEGSTRPGNITTRIRTPETGSDEAIKSILEQAKRELQVQKAEESNIDLKPMRMTKQFESSQPPSSSSNSDENIRSILEQARREMEAQQVALEPVLKASSMGPTDLSLLGSKLLGSPLSTLPYTPITQSLKKPSSSPLLDFHHGGVKKEMGEVAVSDVGLDGVVPKLGRLSESGLGGGSWREHWWSTGLSERRGSSQPSVSEDAHSQEDSKEKLPRLGSTAPVAPASSLDYWKDWPSSESPYSQSSELSLQMPSGSETPQSSPLPSSSPLLSLPKVAKPVVPPLTPEQYEYYMYQEVDTVDLTQQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKHWSKLTQKGREPFIRMQLWLNGELGQALMPMPGQTQEITPKTSASCSPAPESPLSSAEESVKSQQEEQMCRSSVQEPSEASESQPGTPLPLPLPGQAGLSIQEMVAMSAELDTYVITKKVKEVLTDNNLGQRLFGESILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLSDPRNVEKLMDMKRMEKKAYMKRRHSSIGESHSLESGLSAADLTQGASPQHLSQQQPPQQMQPQQQQPQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEELASQLNLKTSTVINWFHNYRSRIRRELFIEEIQAAAGEGGSPSARPGKSGGEGENSCDGTESDSTAEGRPSGSEERKGFLESPGLGHREEDGEGEFPSASGTSRPRGGIIDSLFGIPESISVTNAATTPCRNPKDSSLRKKKAANLNNIIHRLEKAANRDEPHEWEF

Target ORF information:

RefSeq Version	XM_005172721.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172721.4

ORF Insert Sequence:

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGAGAACCC AGGCCAGAAC CTGAACAGAG TGTTTCAGGA TGTTCCGAAG 
CGAAGAGGCG GCTCTGAAGG CTCCACGCGG CCAGGTAACA TCACCACACG GATCCGCACG 
CCTGAAACCG GCTCCGATGA AGCCATCAAG TCCATCTTAG AGCAGGCCAA GAGAGAGTTA 
CAAGTGCAGA AAGCAGAAGA ATCTAACATA GATCTGAAAC CCATGAGGAT GACTAAGCAA 
TTCGAGAGCT CTCAGCCTCC GTCCTCCTCC AGCAACTCGG ACGAGAACAT TCGCTCCATC 
CTCGAGCAGG CTCGCAGGGA GATGGAGGCG CAGCAGGTGG CACTGGAGCC CGTCTTGAAG 
GCCAGCTCCA TGGGCCCGAC GGACCTCTCG CTCCTGGGCT CCAAACTCCT GGGCTCCCCG 
CTCTCCACCC TGCCCTACAC CCCCATCACC CAGTCCCTGA AGAAGCCCTC TAGTTCACCG 
CTGCTGGACT TCCACCATGG TGGAGTGAAG AAGGAGATGG GCGAGGTGGC CGTTTCGGAT 
GTGGGTCTGG ACGGAGTGGT GCCCAAGCTG GGCCGTTTGT CTGAGAGCGG TTTGGGTGGT 
GGCTCGTGGA GGGAGCACTG GTGGAGCACA GGCCTGTCAG AGCGCCGCGG GTCCAGCCAG 
CCCTCCGTTA GTGAAGACGC ACACAGTCAA GAGGACAGCA AAGAGAAGCT CCCTCGGCTG 
GGCAGCACTG CGCCGGTGGC TCCTGCGTCG TCTCTGGATT ACTGGAAGGA CTGGCCGAGC 
TCAGAATCGC CGTACTCTCA GAGCTCAGAG CTCAGCCTGC AGATGCCCAG TGGCAGTGAG 
ACCCCTCAGA GCAGCCCACT GCCTTCCTCC TCGCCCTTAC TGTCCCTGCC CAAAGTGGCC 
AAACCTGTGG TGCCGCCGCT TACGCCAGAG CAATACGAGT ACTACATGTA CCAGGAGGTG 
GACACTGTCG ACCTCACACA GCAGGTCAAA GAAAAACTCG CCAAGAACGG CATCTGCCAG 
CGCATCTTCG GGGAGAAGGT GCTGGGTCTC TCTCAGGGGA GTGTGAGCGA CATGCTCTCT 
CGACCAAAGC ACTGGAGCAA ACTCACACAG AAGGGAAGGG AGCCGTTTAT ACGCATGCAG 
TTATGGCTGA ACGGAGAGCT GGGACAGGCA CTGATGCCAA TGCCTGGACA GACACAAGAA 
ATCACCCCCA AAACCTCGGC CAGCTGCAGT CCTGCCCCAG AATCCCCCCT GAGCTCGGCT 
GAGGAGTCTG TGAAGAGCCA GCAGGAGGAG CAGATGTGCC GATCCTCCGT CCAGGAGCCC 
AGCGAAGCCT CCGAGTCTCA GCCCGGGACG CCGCTGCCGC TGCCTCTGCC GGGACAGGCC 
GGTCTCAGCA TTCAGGAGAT GGTGGCCATG TCAGCCGAGC TGGACACGTA CGTCATCACG 
AAAAAAGTCA AAGAAGTCCT CACTGATAAC AACCTGGGCC AGCGTCTGTT CGGGGAGAGC 
ATTCTGGGTT TGACGCAGGG CTCAGTGTCT GATCTGCTGG CTCGACCCAA ACCCTGGCAC 
AAGCTCAGCC TGAAGGGTCG CGAGCCGTTC GTGCGCATGC AGCTGTGGTT GAGCGACCCG 
CGCAACGTGG AGAAGCTGAT GGACATGAAG CGCATGGAGA AGAAAGCCTA CATGAAAAGG 
CGCCACAGCT CCATCGGTGA GAGTCATTCG CTGGAGAGCG GCTTGTCTGC AGCAGACCTG 
ACACAGGGTG CCAGTCCCCA ACACCTATCC CAGCAGCAGC CGCCGCAGCA GATGCAGCCT 
CAACAGCAGC AGCCCCAGCT GAAGAAACCC CGGGTGGTTC TGGCTCCGGA AGAGAAGGAA 
GCTCTGAAGC GGGCCTACCA GCAGAAGCCT TACCCCTCCC CTAAAACCAT TGAGGAGTTA 
GCGAGTCAGC TCAACCTCAA AACCAGCACC GTCATCAACT GGTTCCACAA CTACAGGTCT 
CGTATCCGGC GAGAGCTTTT TATCGAAGAG ATCCAGGCAG CAGCAGGTGA AGGTGGATCT 
CCATCTGCAC GGCCTGGAAA ATCTGGAGGC GAGGGAGAAA ACAGCTGCGA CGGCACTGAG 
TCCGACAGCA CGGCAGAAGG CCGACCGAGT GGCTCAGAGG AGCGCAAGGG CTTCCTGGAG 
AGCCCGGGCC TCGGCCACAG AGAAGAAGAT GGCGAGGGCG AGTTTCCTAG TGCCTCGGGC 
ACCAGCCGGC CACGAGGAGG CATCATAGAC AGCCTGTTCG GCATCCCCGA GTCCATATCC 
GTCACCAATG CTGCCACGAC CCCCTGCCGA AATCCAAAGG ATAGCAGTCT ACGCAAGAAG 
AAAGCAGCCA ACCTCAACAA CATCATCCAC CGGCTGGAGA AAGCGGCGAA CCGAGACGAG 
CCTCACGAAT GGGAGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cux1a ( XM_005172721.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172778.1 Danio rerio cut-like homeobox 1a (cux1a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa16299
Clone ID Related Accession (Same CDS sequence)	XM_005172721.4 , XM_005172721.3
Accession Version	XM_005172721.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	homeobox protein cut-like 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172721.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGAGAACCC AGGCCAGAAC CTGAACAGAG TGTTTCAGGA TGTTCCGAAG 
CGAAGAGGCG GCTCTGAAGG CTCCACGCGG CCAGGTAACA TCACCACACG GATCCGCACG 
CCTGAAACCG GCTCCGATGA AGCCATCAAG TCCATCTTAG AGCAGGCCAA GAGAGAGTTA 
CAAGTGCAGA AAGCAGAAGA ATCTAACATA GATCTGAAAC CCATGAGGAT GACTAAGCAA 
TTCGAGAGCT CTCAGCCTCC GTCCTCCTCC AGCAACTCGG ACGAGAACAT TCGCTCCATC 
CTCGAGCAGG CTCGCAGGGA GATGGAGGCG CAGCAGGTGG CACTGGAGCC CGTCTTGAAG 
GCCAGCTCCA TGGGCCCGAC GGACCTCTCG CTCCTGGGCT CCAAACTCCT GGGCTCCCCG 
CTCTCCACCC TGCCCTACAC CCCCATCACC CAGTCCCTGA AGAAGCCCTC TAGTTCACCG 
CTGCTGGACT TCCACCATGG TGGAGTGAAG AAGGAGATGG GCGAGGTGGC CGTTTCGGAT 
GTGGGTCTGG ACGGAGTGGT GCCCAAGCTG GGCCGTTTGT CTGAGAGCGG TTTGGGTGGT 
GGCTCGTGGA GGGAGCACTG GTGGAGCACA GGCCTGTCAG AGCGCCGCGG GTCCAGCCAG 
CCCTCCGTTA GTGAAGACGC ACACAGTCAA GAGGACAGCA AAGAGAAGCT CCCTCGGCTG 
GGCAGCACTG CGCCGGTGGC TCCTGCGTCG TCTCTGGATT ACTGGAAGGA CTGGCCGAGC 
TCAGAATCGC CGTACTCTCA GAGCTCAGAG CTCAGCCTGC AGATGCCCAG TGGCAGTGAG 
ACCCCTCAGA GCAGCCCACT GCCTTCCTCC TCGCCCTTAC TGTCCCTGCC CAAAGTGGCC 
AAACCTGTGG TGCCGCCGCT TACGCCAGAG CAATACGAGT ACTACATGTA CCAGGAGGTG 
GACACTGTCG ACCTCACACA GCAGGTCAAA GAAAAACTCG CCAAGAACGG CATCTGCCAG 
CGCATCTTCG GGGAGAAGGT GCTGGGTCTC TCTCAGGGGA GTGTGAGCGA CATGCTCTCT 
CGACCAAAGC ACTGGAGCAA ACTCACACAG AAGGGAAGGG AGCCGTTTAT ACGCATGCAG 
TTATGGCTGA ACGGAGAGCT GGGACAGGCA CTGATGCCAA TGCCTGGACA GACACAAGAA 
ATCACCCCCA AAACCTCGGC CAGCTGCAGT CCTGCCCCAG AATCCCCCCT GAGCTCGGCT 
GAGGAGTCTG TGAAGAGCCA GCAGGAGGAG CAGATGTGCC GATCCTCCGT CCAGGAGCCC 
AGCGAAGCCT CCGAGTCTCA GCCCGGGACG CCGCTGCCGC TGCCTCTGCC GGGACAGGCC 
GGTCTCAGCA TTCAGGAGAT GGTGGCCATG TCAGCCGAGC TGGACACGTA CGTCATCACG 
AAAAAAGTCA AAGAAGTCCT CACTGATAAC AACCTGGGCC AGCGTCTGTT CGGGGAGAGC 
ATTCTGGGTT TGACGCAGGG CTCAGTGTCT GATCTGCTGG CTCGACCCAA ACCCTGGCAC 
AAGCTCAGCC TGAAGGGTCG CGAGCCGTTC GTGCGCATGC AGCTGTGGTT GAGCGACCCG 
CGCAACGTGG AGAAGCTGAT GGACATGAAG CGCATGGAGA AGAAAGCCTA CATGAAAAGG 
CGCCACAGCT CCATCGGTGA GAGTCATTCG CTGGAGAGCG GCTTGTCTGC AGCAGACCTG 
ACACAGGGTG CCAGTCCCCA ACACCTATCC CAGCAGCAGC CGCCGCAGCA GATGCAGCCT 
CAACAGCAGC AGCCCCAGCT GAAGAAACCC CGGGTGGTTC TGGCTCCGGA AGAGAAGGAA 
GCTCTGAAGC GGGCCTACCA GCAGAAGCCT TACCCCTCCC CTAAAACCAT TGAGGAGTTA 
GCGAGTCAGC TCAACCTCAA AACCAGCACC GTCATCAACT GGTTCCACAA CTACAGGTCT 
CGTATCCGGC GAGAGCTTTT TATCGAAGAG ATCCAGGCAG CAGCAGGTGA AGGTGGATCT 
CCATCTGCAC GGCCTGGAAA ATCTGGAGGC GAGGGAGAAA ACAGCTGCGA CGGCACTGAG 
TCCGACAGCA CGGCAGAAGG CCGACCGAGT GGCTCAGAGG AGCGCAAGGG CTTCCTGGAG 
AGCCCGGGCC TCGGCCACAG AGAAGAAGAT GGCGAGGGCG AGTTTCCTAG TGCCTCGGGC 
ACCAGCCGGC CACGAGGAGG CATCATAGAC AGCCTGTTCG GCATCCCCGA GTCCATATCC 
GTCACCAATG CTGCCACGAC CCCCTGCCGA AATCCAAAGG ATAGCAGTCT ACGCAAGAAG 
AAAGCAGCCA ACCTCAACAA CATCATCCAC CGGCTGGAGA AAGCGGCGAA CCGAGACGAG 
CCTCACGAAT GGGAGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172778.1
CDS	182..4582
Translation	MAANAGSMFQYWKRFDLQQLQKELDATATALANRQDESEQSRKKLIDQSREFKKNTPEDLRKQIAPLLKGFQAEIDALSKRSKESEAAFLSVYKRLIDVPDPVSALEAAQQLQVTVRKMHDLETENQRLKDTLQEYEREISEVKNQEVTIKALKEKLEQYERSLQAKNTEEAVEPNLDSSEREERQSKGENLPNNYADKESQPEQSQESRTEDSDVKTQSLQTALEATQAELHELKTKYDEESTAKANEIEAVMTDLERANQRAETAEREAGTLREQLTSAGKTMQSSNQIQNAPEMDKSLEAELGAKEREVERLAEDVQRLQASLAQLSETSTNQISQLEQQLSSKEALLKQLEEKLQDQSDYEEIKRELCTLKSVEHNSSDHHSSAQDSSKPLEMLLTGKDHSQLSESALKRLANSDFTGAVGRKLKETTVENPQRLPATPVPTTLQGSPPPPSPQLLMRTGTATSESAASANGTHPFSPTGIGPDFFTPGMASVGATFPPLAGKMALNSLLQRQLMQTIYTKALQDPSPSSVLFGPPHYATTNSLSSPLPPQSAGSPEVNGMAPSPSQSEGAGSTSEGEEMDTAEIARQVKEQLIKHNIGQRVFGHYVLGLSQGSVSEILARPKPWSKLTIRGKEPFHKMKHFLSDEQNILALRSIQGRQRENPGQNLNRVFQDVPKRRGGSEGSTRPGNITTRIRTPETGSDEAIKSILEQAKRELQVQKAEESNIDLKPMRMTKQFESSQPPSSSSNSDENIRSILEQARREMEAQQVALEPVLKASSMGPTDLSLLGSKLLGSPLSTLPYTPITQSLKKPSSSPLLDFHHGGVKKEMGEVAVSDVGLDGVVPKLGRLSESGLGGGSWREHWWSTGLSERRGSSQPSVSEDAHSQEDSKEKLPRLGSTAPVAPASSLDYWKDWPSSESPYSQSSELSLQMPSGSETPQSSPLPSSSPLLSLPKVAKPVVPPLTPEQYEYYMYQEVDTVDLTQQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKHWSKLTQKGREPFIRMQLWLNGELGQALMPMPGQTQEITPKTSASCSPAPESPLSSAEESVKSQQEEQMCRSSVQEPSEASESQPGTPLPLPLPGQAGLSIQEMVAMSAELDTYVITKKVKEVLTDNNLGQRLFGESILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLSDPRNVEKLMDMKRMEKKAYMKRRHSSIGESHSLESGLSAADLTQGASPQHLSQQQPPQQMQPQQQQPQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEELASQLNLKTSTVINWFHNYRSRIRRELFIEEIQAAAGEGGSPSARPGKSGGEGENSCDGTESDSTAEGRPSGSEERKGFLESPGLGHREEDGEGEFPSASGTSRPRGGIIDSLFGIPESISVTNAATTPCRNPKDSSLRKKKAANLNNIIHRLEKAANRDEPHEWEF

Target ORF information:

RefSeq Version	XM_005172721.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172721.3

ORF Insert Sequence:

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGAGAACCC AGGCCAGAAC CTGAACAGAG TGTTTCAGGA TGTTCCGAAG 
CGAAGAGGCG GCTCTGAAGG CTCCACGCGG CCAGGTAACA TCACCACACG GATCCGCACG 
CCTGAAACCG GCTCCGATGA AGCCATCAAG TCCATCTTAG AGCAGGCCAA GAGAGAGTTA 
CAAGTGCAGA AAGCAGAAGA ATCTAACATA GATCTGAAAC CCATGAGGAT GACTAAGCAA 
TTCGAGAGCT CTCAGCCTCC GTCCTCCTCC AGCAACTCGG ACGAGAACAT TCGCTCCATC 
CTCGAGCAGG CTCGCAGGGA GATGGAGGCG CAGCAGGTGG CACTGGAGCC CGTCTTGAAG 
GCCAGCTCCA TGGGCCCGAC GGACCTCTCG CTCCTGGGCT CCAAACTCCT GGGCTCCCCG 
CTCTCCACCC TGCCCTACAC CCCCATCACC CAGTCCCTGA AGAAGCCCTC TAGTTCACCG 
CTGCTGGACT TCCACCATGG TGGAGTGAAG AAGGAGATGG GCGAGGTGGC CGTTTCGGAT 
GTGGGTCTGG ACGGAGTGGT GCCCAAGCTG GGCCGTTTGT CTGAGAGCGG TTTGGGTGGT 
GGCTCGTGGA GGGAGCACTG GTGGAGCACA GGCCTGTCAG AGCGCCGCGG GTCCAGCCAG 
CCCTCCGTTA GTGAAGACGC ACACAGTCAA GAGGACAGCA AAGAGAAGCT CCCTCGGCTG 
GGCAGCACTG CGCCGGTGGC TCCTGCGTCG TCTCTGGATT ACTGGAAGGA CTGGCCGAGC 
TCAGAATCGC CGTACTCTCA GAGCTCAGAG CTCAGCCTGC AGATGCCCAG TGGCAGTGAG 
ACCCCTCAGA GCAGCCCACT GCCTTCCTCC TCGCCCTTAC TGTCCCTGCC CAAAGTGGCC 
AAACCTGTGG TGCCGCCGCT TACGCCAGAG CAATACGAGT ACTACATGTA CCAGGAGGTG 
GACACTGTCG ACCTCACACA GCAGGTCAAA GAAAAACTCG CCAAGAACGG CATCTGCCAG 
CGCATCTTCG GGGAGAAGGT GCTGGGTCTC TCTCAGGGGA GTGTGAGCGA CATGCTCTCT 
CGACCAAAGC ACTGGAGCAA ACTCACACAG AAGGGAAGGG AGCCGTTTAT ACGCATGCAG 
TTATGGCTGA ACGGAGAGCT GGGACAGGCA CTGATGCCAA TGCCTGGACA GACACAAGAA 
ATCACCCCCA AAACCTCGGC CAGCTGCAGT CCTGCCCCAG AATCCCCCCT GAGCTCGGCT 
GAGGAGTCTG TGAAGAGCCA GCAGGAGGAG CAGATGTGCC GATCCTCCGT CCAGGAGCCC 
AGCGAAGCCT CCGAGTCTCA GCCCGGGACG CCGCTGCCGC TGCCTCTGCC GGGACAGGCC 
GGTCTCAGCA TTCAGGAGAT GGTGGCCATG TCAGCCGAGC TGGACACGTA CGTCATCACG 
AAAAAAGTCA AAGAAGTCCT CACTGATAAC AACCTGGGCC AGCGTCTGTT CGGGGAGAGC 
ATTCTGGGTT TGACGCAGGG CTCAGTGTCT GATCTGCTGG CTCGACCCAA ACCCTGGCAC 
AAGCTCAGCC TGAAGGGTCG CGAGCCGTTC GTGCGCATGC AGCTGTGGTT GAGCGACCCG 
CGCAACGTGG AGAAGCTGAT GGACATGAAG CGCATGGAGA AGAAAGCCTA CATGAAAAGG 
CGCCACAGCT CCATCGGTGA GAGTCATTCG CTGGAGAGCG GCTTGTCTGC AGCAGACCTG 
ACACAGGGTG CCAGTCCCCA ACACCTATCC CAGCAGCAGC CGCCGCAGCA GATGCAGCCT 
CAACAGCAGC AGCCCCAGCT GAAGAAACCC CGGGTGGTTC TGGCTCCGGA AGAGAAGGAA 
GCTCTGAAGC GGGCCTACCA GCAGAAGCCT TACCCCTCCC CTAAAACCAT TGAGGAGTTA 
GCGAGTCAGC TCAACCTCAA AACCAGCACC GTCATCAACT GGTTCCACAA CTACAGGTCT 
CGTATCCGGC GAGAGCTTTT TATCGAAGAG ATCCAGGCAG CAGCAGGTGA AGGTGGATCT 
CCATCTGCAC GGCCTGGAAA ATCTGGAGGC GAGGGAGAAA ACAGCTGCGA CGGCACTGAG 
TCCGACAGCA CGGCAGAAGG CCGACCGAGT GGCTCAGAGG AGCGCAAGGG CTTCCTGGAG 
AGCCCGGGCC TCGGCCACAG AGAAGAAGAT GGCGAGGGCG AGTTTCCTAG TGCCTCGGGC 
ACCAGCCGGC CACGAGGAGG CATCATAGAC AGCCTGTTCG GCATCCCCGA GTCCATATCC 
GTCACCAATG CTGCCACGAC CCCCTGCCGA AATCCAAAGG ATAGCAGTCT ACGCAAGAAG 
AAAGCAGCCA ACCTCAACAA CATCATCCAC CGGCTGGAGA AAGCGGCGAA CCGAGACGAG 
CCTCACGAAT GGGAGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cux1a ( XM_003199366.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003199414.2 Danio rerio cut-like homeobox 1a (cux1a), transcript variant X5, mRNA.

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CloneID	ODa16300
Clone ID Related Accession (Same CDS sequence)	XM_003199366.4 , XM_003199366.5
Accession Version	XM_003199366.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	homeobox protein cut-like 1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003199366.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGAGAACCC AGGCCAGAAC CTGAACAGAG TGTTTCAGGA TGTTCCGAAG 
CGAAGAGGCG GCTCTGAAGG CTCCACGCGG CCAGGTAACA TCACCACACG GATCCGCACG 
CCTGAAACCG GCTCCGATGA AGCCATCAAG TCCATCTTAG AGCAGGCCAA GAGAGAGTTA 
CAAGTGCAGA AAGCAGTCGA GAGCTCTCAG CCTCCGTCCT CCTCCAGCAA CTCGGACGAG 
AACATTCGCT CCATCCTCGA GCAGGCTCGC AGGGAGATGG AGGCGCAGCA GGTGGCACTG 
GAGCCCGTCT TGAAGGCCAG CTCCATGGGC CCGACGGACC TCTCGCTCCT GGGCTCCAAA 
CTCCTGGGCT CCCCGCTCTC CACCCTGCCC TACACCCCCA TCACCCAGTC CCTGAAGAAG 
CCCTCTAGTT CACCGCTGCT GGACTTCCAC CATGGTGGAG TGAAGAAGGA GATGGGCGAG 
GTGGCCGTTT CGGATGTGGG TCTGGACGGA GTGGTGCCCA AGCTGGGCCG TTTGTCTGAG 
AGCGGTTTGG GTGGTGGCTC GTGGAGGGAG CACTGGTGGA GCACAGGCCT GTCAGAGCGC 
CGCGGGTCCA GCCAGCCCTC CGTTAGTGAA GACGCACACA GTCAAGAGGA CAGCAAAGAG 
AAGCTCCCTC GGCTGGGCAG CACTGCGCCG GTGGCTCCTG CGTCGTCTCT GGATTACTGG 
AAGGACTGGC CGAGCTCAGA ATCGCCGTAC TCTCAGAGCT CAGAGCTCAG CCTGCAGATG 
CCCAGTGGCA GTGAGACCCC TCAGAGCAGC CCACTGCCTT CCTCCTCGCC CTTACTGTCC 
CTGCCCAAAG TGGCCAAACC TGTGGTGCCG CCGCTTACGC CAGAGCAATA CGAGTACTAC 
ATGTACCAGG AGGTGGACAC TGTCGACCTC ACACAGCAGG TCAAAGAAAA ACTCGCCAAG 
AACGGCATCT GCCAGCGCAT CTTCGGGGAG AAGGTGCTGG GTCTCTCTCA GGGGAGTGTG 
AGCGACATGC TCTCTCGACC AAAGCACTGG AGCAAACTCA CACAGAAGGG AAGGGAGCCG 
TTTATACGCA TGCAGTTATG GCTGAACGGA GAGCTGGGAC AGGCACTGAT GCCAATGCCT 
GGACAGACAC AAGAAATCAC CCCCAAAACC TCGGCCAGCT GCAGTCCTGC CCCAGAATCC 
CCCCTGAGCT CGGCTGAGGA GTCTGTGAAG AGCCAGCAGG AGGAGCAGAT GTGCCGATCC 
TCCGTCCAGG AGCCCAGCGA AGCCTCCGAG TCTCAGCCCG GGACGCCGCT GCCGCTGCCT 
CTGCCGGGAC AGGCCGGTCT CAGCATTCAG GAGATGGTGG CCATGTCAGC CGAGCTGGAC 
ACGTACGTCA TCACGAAAAA AGTCAAAGAA GTCCTCACTG ATAACAACCT GGGCCAGCGT 
CTGTTCGGGG AGAGCATTCT GGGTTTGACG CAGGGCTCAG TGTCTGATCT GCTGGCTCGA 
CCCAAACCCT GGCACAAGCT CAGCCTGAAG GGTCGCGAGC CGTTCGTGCG CATGCAGCTG 
TGGTTGAGCG ACCCGCGCAA CGTGGAGAAG CTGATGGACA TGAAGCGCAT GGAGAAGAAA 
GCCTACATGA AAAGGCGCCA CAGCTCCATC GGTGAGAGTC ATTCGCTGGA GAGCGGCTTG 
TCTGCAGCAG ACCTGACACA GGGTGCCAGT CCCCAACACC TATCCCAGCA GCAGCCGCCG 
CAGCAGATGC AGCCTCAACA GCAGCAGCCC CAGCTGAAGA AACCCCGGGT GGTTCTGGCT 
CCGGAAGAGA AGGAAGCTCT GAAGCGGGCC TACCAGCAGA AGCCTTACCC CTCCCCTAAA 
ACCATTGAGG AGTTAGCGAG TCAGCTCAAC CTCAAAACCA GCACCGTCAT CAACTGGTTC 
CACAACTACA GGTCTCGTAT CCGGCGAGAG CTTTTTATCG AAGAGATCCA GGCAGCAGCA 
GGTGAAGGTG GATCTCCATC TGCACGGCCT GGAAAATCTG GAGGCGAGGG AGAAAACAGC 
TGCGACGGCA CTGAGTCCGA CAGCACGGCA GAAGGCCGAC CGAGTGGCTC AGAGGAGCGC 
AAGGGCTTCC TGGAGAGCCC GGGCCTCGGC CACAGAGAAG AAGATGGCGA GGGCGAGTTT 
CCTAGTGCCT CGGGCACCAG CCGGCCACGA GGAGGCATCA TAGACAGCCT GTTCGGCATC 
CCCGAGTCCA TATCCGTCAC CAATGCTGCC ACGACCCCCT GCCGAAATCC AAAGGATAGC 
AGTCTACGCA AGAAGAAAGC AGCCAACCTC AACAACATCA TCCACCGGCT GGAGAAAGCG 
GCGAACCGAG ACGAGCCTCA CGAATGGGAG TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003199414.2
CDS	180..4535
Translation	MAANAGSMFQYWKRFDLQQLQKELDATATALANRQDESEQSRKKLIDQSREFKKNTPEDLRKQIAPLLKGFQAEIDALSKRSKESEAAFLSVYKRLIDVPDPVSALEAAQQLQVTVRKMHDLETENQRLKDTLQEYEREISEVKNQEVTIKALKEKLEQYERSLQAKNTEEAVEPNLDSSEREERQSKGENLPNNYADKESQPEQSQESRTEDSDVKTQSLQTALEATQAELHELKTKYDEESTAKANEIEAVMTDLERANQRAETAEREAGTLREQLTSAGKTMQSSNQIQNAPEMDKSLEAELGAKEREVERLAEDVQRLQASLAQLSETSTNQISQLEQQLSSKEALLKQLEEKLQDQSDYEEIKRELCTLKSVEHNSSDHHSSAQDSSKPLEMLLTGKDHSQLSESALKRLANSDFTGAVGRKLKETTVENPQRLPATPVPTTLQGSPPPPSPQLLMRTGTATSESAASANGTHPFSPTGIGPDFFTPGMASVGATFPPLAGKMALNSLLQRQLMQTIYTKALQDPSPSSVLFGPPHYATTNSLSSPLPPQSAGSPEVNGMAPSPSQSEGAGSTSEGEEMDTAEIARQVKEQLIKHNIGQRVFGHYVLGLSQGSVSEILARPKPWSKLTIRGKEPFHKMKHFLSDEQNILALRSIQGRQRENPGQNLNRVFQDVPKRRGGSEGSTRPGNITTRIRTPETGSDEAIKSILEQAKRELQVQKAVESSQPPSSSSNSDENIRSILEQARREMEAQQVALEPVLKASSMGPTDLSLLGSKLLGSPLSTLPYTPITQSLKKPSSSPLLDFHHGGVKKEMGEVAVSDVGLDGVVPKLGRLSESGLGGGSWREHWWSTGLSERRGSSQPSVSEDAHSQEDSKEKLPRLGSTAPVAPASSLDYWKDWPSSESPYSQSSELSLQMPSGSETPQSSPLPSSSPLLSLPKVAKPVVPPLTPEQYEYYMYQEVDTVDLTQQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKHWSKLTQKGREPFIRMQLWLNGELGQALMPMPGQTQEITPKTSASCSPAPESPLSSAEESVKSQQEEQMCRSSVQEPSEASESQPGTPLPLPLPGQAGLSIQEMVAMSAELDTYVITKKVKEVLTDNNLGQRLFGESILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLSDPRNVEKLMDMKRMEKKAYMKRRHSSIGESHSLESGLSAADLTQGASPQHLSQQQPPQQMQPQQQQPQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEELASQLNLKTSTVINWFHNYRSRIRRELFIEEIQAAAGEGGSPSARPGKSGGEGENSCDGTESDSTAEGRPSGSEERKGFLESPGLGHREEDGEGEFPSASGTSRPRGGIIDSLFGIPESISVTNAATTPCRNPKDSSLRKKKAANLNNIIHRLEKAANRDEPHEWEF

Target ORF information:

RefSeq Version	XM_003199366.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003199366.4

ORF Insert Sequence:

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGAGAACCC AGGCCAGAAC CTGAACAGAG TGTTTCAGGA TGTTCCGAAG 
CGAAGAGGCG GCTCTGAAGG CTCCACGCGG CCAGGTAACA TCACCACACG GATCCGCACG 
CCTGAAACCG GCTCCGATGA AGCCATCAAG TCCATCTTAG AGCAGGCCAA GAGAGAGTTA 
CAAGTGCAGA AAGCAGTCGA GAGCTCTCAG CCTCCGTCCT CCTCCAGCAA CTCGGACGAG 
AACATTCGCT CCATCCTCGA GCAGGCTCGC AGGGAGATGG AGGCGCAGCA GGTGGCACTG 
GAGCCCGTCT TGAAGGCCAG CTCCATGGGC CCGACGGACC TCTCGCTCCT GGGCTCCAAA 
CTCCTGGGCT CCCCGCTCTC CACCCTGCCC TACACCCCCA TCACCCAGTC CCTGAAGAAG 
CCCTCTAGTT CACCGCTGCT GGACTTCCAC CATGGTGGAG TGAAGAAGGA GATGGGCGAG 
GTGGCCGTTT CGGATGTGGG TCTGGACGGA GTGGTGCCCA AGCTGGGCCG TTTGTCTGAG 
AGCGGTTTGG GTGGTGGCTC GTGGAGGGAG CACTGGTGGA GCACAGGCCT GTCAGAGCGC 
CGCGGGTCCA GCCAGCCCTC CGTTAGTGAA GACGCACACA GTCAAGAGGA CAGCAAAGAG 
AAGCTCCCTC GGCTGGGCAG CACTGCGCCG GTGGCTCCTG CGTCGTCTCT GGATTACTGG 
AAGGACTGGC CGAGCTCAGA ATCGCCGTAC TCTCAGAGCT CAGAGCTCAG CCTGCAGATG 
CCCAGTGGCA GTGAGACCCC TCAGAGCAGC CCACTGCCTT CCTCCTCGCC CTTACTGTCC 
CTGCCCAAAG TGGCCAAACC TGTGGTGCCG CCGCTTACGC CAGAGCAATA CGAGTACTAC 
ATGTACCAGG AGGTGGACAC TGTCGACCTC ACACAGCAGG TCAAAGAAAA ACTCGCCAAG 
AACGGCATCT GCCAGCGCAT CTTCGGGGAG AAGGTGCTGG GTCTCTCTCA GGGGAGTGTG 
AGCGACATGC TCTCTCGACC AAAGCACTGG AGCAAACTCA CACAGAAGGG AAGGGAGCCG 
TTTATACGCA TGCAGTTATG GCTGAACGGA GAGCTGGGAC AGGCACTGAT GCCAATGCCT 
GGACAGACAC AAGAAATCAC CCCCAAAACC TCGGCCAGCT GCAGTCCTGC CCCAGAATCC 
CCCCTGAGCT CGGCTGAGGA GTCTGTGAAG AGCCAGCAGG AGGAGCAGAT GTGCCGATCC 
TCCGTCCAGG AGCCCAGCGA AGCCTCCGAG TCTCAGCCCG GGACGCCGCT GCCGCTGCCT 
CTGCCGGGAC AGGCCGGTCT CAGCATTCAG GAGATGGTGG CCATGTCAGC CGAGCTGGAC 
ACGTACGTCA TCACGAAAAA AGTCAAAGAA GTCCTCACTG ATAACAACCT GGGCCAGCGT 
CTGTTCGGGG AGAGCATTCT GGGTTTGACG CAGGGCTCAG TGTCTGATCT GCTGGCTCGA 
CCCAAACCCT GGCACAAGCT CAGCCTGAAG GGTCGCGAGC CGTTCGTGCG CATGCAGCTG 
TGGTTGAGCG ACCCGCGCAA CGTGGAGAAG CTGATGGACA TGAAGCGCAT GGAGAAGAAA 
GCCTACATGA AAAGGCGCCA CAGCTCCATC GGTGAGAGTC ATTCGCTGGA GAGCGGCTTG 
TCTGCAGCAG ACCTGACACA GGGTGCCAGT CCCCAACACC TATCCCAGCA GCAGCCGCCG 
CAGCAGATGC AGCCTCAACA GCAGCAGCCC CAGCTGAAGA AACCCCGGGT GGTTCTGGCT 
CCGGAAGAGA AGGAAGCTCT GAAGCGGGCC TACCAGCAGA AGCCTTACCC CTCCCCTAAA 
ACCATTGAGG AGTTAGCGAG TCAGCTCAAC CTCAAAACCA GCACCGTCAT CAACTGGTTC 
CACAACTACA GGTCTCGTAT CCGGCGAGAG CTTTTTATCG AAGAGATCCA GGCAGCAGCA 
GGTGAAGGTG GATCTCCATC TGCACGGCCT GGAAAATCTG GAGGCGAGGG AGAAAACAGC 
TGCGACGGCA CTGAGTCCGA CAGCACGGCA GAAGGCCGAC CGAGTGGCTC AGAGGAGCGC 
AAGGGCTTCC TGGAGAGCCC GGGCCTCGGC CACAGAGAAG AAGATGGCGA GGGCGAGTTT 
CCTAGTGCCT CGGGCACCAG CCGGCCACGA GGAGGCATCA TAGACAGCCT GTTCGGCATC 
CCCGAGTCCA TATCCGTCAC CAATGCTGCC ACGACCCCCT GCCGAAATCC AAAGGATAGC 
AGTCTACGCA AGAAGAAAGC AGCCAACCTC AACAACATCA TCCACCGGCT GGAGAAAGCG 
GCGAACCGAG ACGAGCCTCA CGAATGGGAG TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cux1a ( XM_003199366.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003199414.2 Danio rerio cut-like homeobox 1a (cux1a), transcript variant X5, mRNA.

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CloneID	ODa16300
Clone ID Related Accession (Same CDS sequence)	XM_003199366.4 , XM_003199366.5
Accession Version	XM_003199366.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	homeobox protein cut-like 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003199366.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGAGAACCC AGGCCAGAAC CTGAACAGAG TGTTTCAGGA TGTTCCGAAG 
CGAAGAGGCG GCTCTGAAGG CTCCACGCGG CCAGGTAACA TCACCACACG GATCCGCACG 
CCTGAAACCG GCTCCGATGA AGCCATCAAG TCCATCTTAG AGCAGGCCAA GAGAGAGTTA 
CAAGTGCAGA AAGCAGTCGA GAGCTCTCAG CCTCCGTCCT CCTCCAGCAA CTCGGACGAG 
AACATTCGCT CCATCCTCGA GCAGGCTCGC AGGGAGATGG AGGCGCAGCA GGTGGCACTG 
GAGCCCGTCT TGAAGGCCAG CTCCATGGGC CCGACGGACC TCTCGCTCCT GGGCTCCAAA 
CTCCTGGGCT CCCCGCTCTC CACCCTGCCC TACACCCCCA TCACCCAGTC CCTGAAGAAG 
CCCTCTAGTT CACCGCTGCT GGACTTCCAC CATGGTGGAG TGAAGAAGGA GATGGGCGAG 
GTGGCCGTTT CGGATGTGGG TCTGGACGGA GTGGTGCCCA AGCTGGGCCG TTTGTCTGAG 
AGCGGTTTGG GTGGTGGCTC GTGGAGGGAG CACTGGTGGA GCACAGGCCT GTCAGAGCGC 
CGCGGGTCCA GCCAGCCCTC CGTTAGTGAA GACGCACACA GTCAAGAGGA CAGCAAAGAG 
AAGCTCCCTC GGCTGGGCAG CACTGCGCCG GTGGCTCCTG CGTCGTCTCT GGATTACTGG 
AAGGACTGGC CGAGCTCAGA ATCGCCGTAC TCTCAGAGCT CAGAGCTCAG CCTGCAGATG 
CCCAGTGGCA GTGAGACCCC TCAGAGCAGC CCACTGCCTT CCTCCTCGCC CTTACTGTCC 
CTGCCCAAAG TGGCCAAACC TGTGGTGCCG CCGCTTACGC CAGAGCAATA CGAGTACTAC 
ATGTACCAGG AGGTGGACAC TGTCGACCTC ACACAGCAGG TCAAAGAAAA ACTCGCCAAG 
AACGGCATCT GCCAGCGCAT CTTCGGGGAG AAGGTGCTGG GTCTCTCTCA GGGGAGTGTG 
AGCGACATGC TCTCTCGACC AAAGCACTGG AGCAAACTCA CACAGAAGGG AAGGGAGCCG 
TTTATACGCA TGCAGTTATG GCTGAACGGA GAGCTGGGAC AGGCACTGAT GCCAATGCCT 
GGACAGACAC AAGAAATCAC CCCCAAAACC TCGGCCAGCT GCAGTCCTGC CCCAGAATCC 
CCCCTGAGCT CGGCTGAGGA GTCTGTGAAG AGCCAGCAGG AGGAGCAGAT GTGCCGATCC 
TCCGTCCAGG AGCCCAGCGA AGCCTCCGAG TCTCAGCCCG GGACGCCGCT GCCGCTGCCT 
CTGCCGGGAC AGGCCGGTCT CAGCATTCAG GAGATGGTGG CCATGTCAGC CGAGCTGGAC 
ACGTACGTCA TCACGAAAAA AGTCAAAGAA GTCCTCACTG ATAACAACCT GGGCCAGCGT 
CTGTTCGGGG AGAGCATTCT GGGTTTGACG CAGGGCTCAG TGTCTGATCT GCTGGCTCGA 
CCCAAACCCT GGCACAAGCT CAGCCTGAAG GGTCGCGAGC CGTTCGTGCG CATGCAGCTG 
TGGTTGAGCG ACCCGCGCAA CGTGGAGAAG CTGATGGACA TGAAGCGCAT GGAGAAGAAA 
GCCTACATGA AAAGGCGCCA CAGCTCCATC GGTGAGAGTC ATTCGCTGGA GAGCGGCTTG 
TCTGCAGCAG ACCTGACACA GGGTGCCAGT CCCCAACACC TATCCCAGCA GCAGCCGCCG 
CAGCAGATGC AGCCTCAACA GCAGCAGCCC CAGCTGAAGA AACCCCGGGT GGTTCTGGCT 
CCGGAAGAGA AGGAAGCTCT GAAGCGGGCC TACCAGCAGA AGCCTTACCC CTCCCCTAAA 
ACCATTGAGG AGTTAGCGAG TCAGCTCAAC CTCAAAACCA GCACCGTCAT CAACTGGTTC 
CACAACTACA GGTCTCGTAT CCGGCGAGAG CTTTTTATCG AAGAGATCCA GGCAGCAGCA 
GGTGAAGGTG GATCTCCATC TGCACGGCCT GGAAAATCTG GAGGCGAGGG AGAAAACAGC 
TGCGACGGCA CTGAGTCCGA CAGCACGGCA GAAGGCCGAC CGAGTGGCTC AGAGGAGCGC 
AAGGGCTTCC TGGAGAGCCC GGGCCTCGGC CACAGAGAAG AAGATGGCGA GGGCGAGTTT 
CCTAGTGCCT CGGGCACCAG CCGGCCACGA GGAGGCATCA TAGACAGCCT GTTCGGCATC 
CCCGAGTCCA TATCCGTCAC CAATGCTGCC ACGACCCCCT GCCGAAATCC AAAGGATAGC 
AGTCTACGCA AGAAGAAAGC AGCCAACCTC AACAACATCA TCCACCGGCT GGAGAAAGCG 
GCGAACCGAG ACGAGCCTCA CGAATGGGAG TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003199414.2
CDS	79..4434
Translation	MAANAGSMFQYWKRFDLQQLQKELDATATALANRQDESEQSRKKLIDQSREFKKNTPEDLRKQIAPLLKGFQAEIDALSKRSKESEAAFLSVYKRLIDVPDPVSALEAAQQLQVTVRKMHDLETENQRLKDTLQEYEREISEVKNQEVTIKALKEKLEQYERSLQAKNTEEAVEPNLDSSEREERQSKGENLPNNYADKESQPEQSQESRTEDSDVKTQSLQTALEATQAELHELKTKYDEESTAKANEIEAVMTDLERANQRAETAEREAGTLREQLTSAGKTMQSSNQIQNAPEMDKSLEAELGAKEREVERLAEDVQRLQASLAQLSETSTNQISQLEQQLSSKEALLKQLEEKLQDQSDYEEIKRELCTLKSVEHNSSDHHSSAQDSSKPLEMLLTGKDHSQLSESALKRLANSDFTGAVGRKLKETTVENPQRLPATPVPTTLQGSPPPPSPQLLMRTGTATSESAASANGTHPFSPTGIGPDFFTPGMASVGATFPPLAGKMALNSLLQRQLMQTIYTKALQDPSPSSVLFGPPHYATTNSLSSPLPPQSAGSPEVNGMAPSPSQSEGAGSTSEGEEMDTAEIARQVKEQLIKHNIGQRVFGHYVLGLSQGSVSEILARPKPWSKLTIRGKEPFHKMKHFLSDEQNILALRSIQGRQRENPGQNLNRVFQDVPKRRGGSEGSTRPGNITTRIRTPETGSDEAIKSILEQAKRELQVQKAVESSQPPSSSSNSDENIRSILEQARREMEAQQVALEPVLKASSMGPTDLSLLGSKLLGSPLSTLPYTPITQSLKKPSSSPLLDFHHGGVKKEMGEVAVSDVGLDGVVPKLGRLSESGLGGGSWREHWWSTGLSERRGSSQPSVSEDAHSQEDSKEKLPRLGSTAPVAPASSLDYWKDWPSSESPYSQSSELSLQMPSGSETPQSSPLPSSSPLLSLPKVAKPVVPPLTPEQYEYYMYQEVDTVDLTQQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKHWSKLTQKGREPFIRMQLWLNGELGQALMPMPGQTQEITPKTSASCSPAPESPLSSAEESVKSQQEEQMCRSSVQEPSEASESQPGTPLPLPLPGQAGLSIQEMVAMSAELDTYVITKKVKEVLTDNNLGQRLFGESILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLSDPRNVEKLMDMKRMEKKAYMKRRHSSIGESHSLESGLSAADLTQGASPQHLSQQQPPQQMQPQQQQPQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEELASQLNLKTSTVINWFHNYRSRIRRELFIEEIQAAAGEGGSPSARPGKSGGEGENSCDGTESDSTAEGRPSGSEERKGFLESPGLGHREEDGEGEFPSASGTSRPRGGIIDSLFGIPESISVTNAATTPCRNPKDSSLRKKKAANLNNIIHRLEKAANRDEPHEWEF

Target ORF information:

RefSeq Version	XM_003199366.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cut-like homeobox 1a (cux1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003199366.5

ORF Insert Sequence:

ATGGCGGCCA ATGCGGGATC GATGTTTCAA TATTGGAAAC GCTTTGATTT GCAGCAGCTC 
CAGAAAGAAC TGGATGCCAC CGCCACAGCC CTGGCCAACC GACAGGATGA GAGCGAGCAG 
TCCAGGAAAA AACTCATCGA CCAAAGCCGC GAGTTCAAGA AGAATACTCC AGAGGATTTG 
CGTAAACAGA TAGCTCCTTT GCTGAAGGGC TTCCAAGCAG AGATCGACGC CTTGAGTAAA 
AGAAGTAAAG AATCAGAGGC TGCTTTCCTC AGCGTGTACA AGAGATTAAT CGATGTCCCA 
GATCCCGTCT CGGCACTGGA AGCAGCTCAG CAACTCCAGG TGACGGTTCG GAAGATGCAC 
GACTTGGAGA CCGAAAACCA GAGGCTCAAA GATACACTAC AAGAGTACGA ACGTGAAATT 
TCAGAGGTTA AAAACCAAGA AGTCACTATT AAAGCACTTA AAGAGAAGCT GGAGCAGTAT 
GAGCGTTCTC TGCAGGCCAA GAACACAGAG GAGGCTGTCG AGCCGAACTT GGACTCATCA 
GAAAGAGAAG AGAGGCAAAG CAAAGGAGAA AACCTTCCAA ACAACTATGC AGACAAGGAG 
AGTCAGCCAG AGCAGAGCCA GGAGAGCAGG ACAGAAGACT CGGATGTGAA AACACAGTCC 
TTACAAACAG CACTTGAAGC AACTCAGGCA GAACTCCACG AACTGAAGAC GAAATATGAT 
GAAGAATCCA CAGCAAAGGC CAATGAGATT GAAGCGGTGA TGACAGACTT GGAAAGAGCC 
AATCAGAGGG CCGAGACGGC AGAGAGGGAG GCCGGGACCC TGAGGGAGCA GCTCACTTCT 
GCTGGAAAGA CCATGCAATC ATCCAATCAG ATCCAGAACG CTCCGGAAAT GGACAAGTCT 
CTGGAGGCAG AGCTGGGTGC GAAAGAGAGG GAGGTGGAGC GTTTGGCTGA GGACGTTCAG 
AGACTTCAGG CCAGTCTGGC TCAGCTCAGC GAGACATCAA CCAACCAGAT CAGCCAGCTC 
GAGCAGCAGC TCAGCTCCAA AGAGGCACTA CTAAAGCAAC TTGAAGAAAA GCTTCAAGAT 
CAGTCAGACT ACGAGGAGAT CAAACGAGAA CTCTGCACCC TGAAATCAGT GGAGCACAAC 
TCATCAGATC ATCACTCTTC AGCGCAGGAC TCATCCAAGC CACTGGAGAT GTTGCTGACA 
GGGAAAGACC ATAGCCAGCT GTCAGAGAGC GCTTTGAAAC GACTGGCCAA CAGTGACTTC 
ACTGGAGCAG TGGGCCGTAA ACTGAAAGAA ACTACAGTGG AAAACCCTCA GCGTCTTCCT 
GCCACTCCGG TCCCGACCAC CCTCCAGGGG TCTCCACCTC CACCCTCCCC TCAGCTGCTG 
ATGAGGACGG GCACCGCTAC CTCCGAATCC GCTGCCAGTG CCAACGGTAC CCACCCTTTC 
TCCCCCACAG GCATCGGGCC GGACTTCTTC ACCCCCGGCA TGGCCAGCGT GGGCGCCACG 
TTCCCCCCGC TGGCTGGCAA AATGGCACTC AACTCTCTTC TTCAGAGGCA GCTCATGCAA 
ACCATCTACA CCAAAGCCCT CCAGGACCCC TCTCCCAGTT CGGTGCTGTT CGGGCCACCC 
CATTACGCTA CCACCAACTC CCTCTCCAGC CCCCTGCCGC CCCAGTCGGC AGGCAGCCCG 
GAGGTTAACG GCATGGCGCC TTCGCCCAGC CAATCAGAGG GCGCGGGCAG CACGTCGGAA 
GGTGAGGAAA TGGATACAGC GGAGATTGCC CGACAGGTCA AGGAGCAGCT GATCAAACAC 
AACATCGGCC AGCGCGTGTT CGGCCATTAC GTGCTGGGTC TCTCCCAAGG CTCCGTTAGC 
GAAATTCTGG CTCGGCCCAA ACCTTGGAGC AAGCTAACCA TCCGTGGCAA AGAGCCGTTT 
CACAAGATGA AGCACTTCCT GTCCGATGAG CAGAACATCC TGGCGCTGAG GAGCATCCAG 
GGCAGGCAGA GAGAGAACCC AGGCCAGAAC CTGAACAGAG TGTTTCAGGA TGTTCCGAAG 
CGAAGAGGCG GCTCTGAAGG CTCCACGCGG CCAGGTAACA TCACCACACG GATCCGCACG 
CCTGAAACCG GCTCCGATGA AGCCATCAAG TCCATCTTAG AGCAGGCCAA GAGAGAGTTA 
CAAGTGCAGA AAGCAGTCGA GAGCTCTCAG CCTCCGTCCT CCTCCAGCAA CTCGGACGAG 
AACATTCGCT CCATCCTCGA GCAGGCTCGC AGGGAGATGG AGGCGCAGCA GGTGGCACTG 
GAGCCCGTCT TGAAGGCCAG CTCCATGGGC CCGACGGACC TCTCGCTCCT GGGCTCCAAA 
CTCCTGGGCT CCCCGCTCTC CACCCTGCCC TACACCCCCA TCACCCAGTC CCTGAAGAAG 
CCCTCTAGTT CACCGCTGCT GGACTTCCAC CATGGTGGAG TGAAGAAGGA GATGGGCGAG 
GTGGCCGTTT CGGATGTGGG TCTGGACGGA GTGGTGCCCA AGCTGGGCCG TTTGTCTGAG 
AGCGGTTTGG GTGGTGGCTC GTGGAGGGAG CACTGGTGGA GCACAGGCCT GTCAGAGCGC 
CGCGGGTCCA GCCAGCCCTC CGTTAGTGAA GACGCACACA GTCAAGAGGA CAGCAAAGAG 
AAGCTCCCTC GGCTGGGCAG CACTGCGCCG GTGGCTCCTG CGTCGTCTCT GGATTACTGG 
AAGGACTGGC CGAGCTCAGA ATCGCCGTAC TCTCAGAGCT CAGAGCTCAG CCTGCAGATG 
CCCAGTGGCA GTGAGACCCC TCAGAGCAGC CCACTGCCTT CCTCCTCGCC CTTACTGTCC 
CTGCCCAAAG TGGCCAAACC TGTGGTGCCG CCGCTTACGC CAGAGCAATA CGAGTACTAC 
ATGTACCAGG AGGTGGACAC TGTCGACCTC ACACAGCAGG TCAAAGAAAA ACTCGCCAAG 
AACGGCATCT GCCAGCGCAT CTTCGGGGAG AAGGTGCTGG GTCTCTCTCA GGGGAGTGTG 
AGCGACATGC TCTCTCGACC AAAGCACTGG AGCAAACTCA CACAGAAGGG AAGGGAGCCG 
TTTATACGCA TGCAGTTATG GCTGAACGGA GAGCTGGGAC AGGCACTGAT GCCAATGCCT 
GGACAGACAC AAGAAATCAC CCCCAAAACC TCGGCCAGCT GCAGTCCTGC CCCAGAATCC 
CCCCTGAGCT CGGCTGAGGA GTCTGTGAAG AGCCAGCAGG AGGAGCAGAT GTGCCGATCC 
TCCGTCCAGG AGCCCAGCGA AGCCTCCGAG TCTCAGCCCG GGACGCCGCT GCCGCTGCCT 
CTGCCGGGAC AGGCCGGTCT CAGCATTCAG GAGATGGTGG CCATGTCAGC CGAGCTGGAC 
ACGTACGTCA TCACGAAAAA AGTCAAAGAA GTCCTCACTG ATAACAACCT GGGCCAGCGT 
CTGTTCGGGG AGAGCATTCT GGGTTTGACG CAGGGCTCAG TGTCTGATCT GCTGGCTCGA 
CCCAAACCCT GGCACAAGCT CAGCCTGAAG GGTCGCGAGC CGTTCGTGCG CATGCAGCTG 
TGGTTGAGCG ACCCGCGCAA CGTGGAGAAG CTGATGGACA TGAAGCGCAT GGAGAAGAAA 
GCCTACATGA AAAGGCGCCA CAGCTCCATC GGTGAGAGTC ATTCGCTGGA GAGCGGCTTG 
TCTGCAGCAG ACCTGACACA GGGTGCCAGT CCCCAACACC TATCCCAGCA GCAGCCGCCG 
CAGCAGATGC AGCCTCAACA GCAGCAGCCC CAGCTGAAGA AACCCCGGGT GGTTCTGGCT 
CCGGAAGAGA AGGAAGCTCT GAAGCGGGCC TACCAGCAGA AGCCTTACCC CTCCCCTAAA 
ACCATTGAGG AGTTAGCGAG TCAGCTCAAC CTCAAAACCA GCACCGTCAT CAACTGGTTC 
CACAACTACA GGTCTCGTAT CCGGCGAGAG CTTTTTATCG AAGAGATCCA GGCAGCAGCA 
GGTGAAGGTG GATCTCCATC TGCACGGCCT GGAAAATCTG GAGGCGAGGG AGAAAACAGC 
TGCGACGGCA CTGAGTCCGA CAGCACGGCA GAAGGCCGAC CGAGTGGCTC AGAGGAGCGC 
AAGGGCTTCC TGGAGAGCCC GGGCCTCGGC CACAGAGAAG AAGATGGCGA GGGCGAGTTT 
CCTAGTGCCT CGGGCACCAG CCGGCCACGA GGAGGCATCA TAGACAGCCT GTTCGGCATC 
CCCGAGTCCA TATCCGTCAC CAATGCTGCC ACGACCCCCT GCCGAAATCC AAAGGATAGC 
AGTCTACGCA AGAAGAAAGC AGCCAACCTC AACAACATCA TCCACCGGCT GGAGAAAGCG 
GCGAACCGAG ACGAGCCTCA CGAATGGGAG TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	cux1a
Entrez Gene ID	797445
Full Name	cut-like homeobox 1a
Synonyms	si:ch211-251d10.5,wu:fi22b07,wu:fk35a11
General protein information	Preferred Names cut-like homeobox 1a Names cut-like homeobox 1 homeobox protein cut-like 1 fi22b07
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Homo sapiens (human)	CUX1	NP_001189472.1
Canis lupus familiaris (dog)	CUX1	XP_005621055.1
Gallus gallus (chicken)	CUX1	XP_004946538.1
Xenopus tropicalis (tropical clawed frog)	cux1	XP_002942311.2
Bos taurus (cattle)	CUX1	XP_005225313.1
Danio rerio (zebrafish)	cux1a	XP_005172775.1
Pan troglodytes (chimpanzee)	CUX1	XP_003949274.1
	CUTL1	XP_001114534.2
Rattus norvegicus (Norway rat)	Cux1	XP_003751208.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

cux1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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