leg1.1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	leg1.1
Entrez Gene ID	796447
Full Name	liver-enriched gene 1, tandem duplicate 1
Synonyms	Leg1-A,leg1a,wu:fb61h05,zgc:172246
General protein information	Preferred Names liver-enriched gene 1, tandem duplicate 1 Names uncharacterized protein LOC796447 leg1 liver-enriched gene protein 1-A protein leg1a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001100056.2	NP_001093526.1	uncharacterized protein LOC796447 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	C9H6orf58	XP_002690103.2
Danio rerio (zebrafish)	LOC796447	NP_001093526.1
Canis lupus familiaris (dog)	LOC612694	XP_005615688.1
Mus musculus (house mouse)	2310057J18Rik	NP_080612.1
Danio rerio (zebrafish)	zgc:77778	NP_998368.1
Rattus norvegicus (Norway rat)	RGD1311933	XP_217725.3
Gallus gallus (chicken)	LOC421712	XP_419749.3
Xenopus tropicalis (tropical clawed frog)	LOC100496643	XP_002940437.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Liver-Enriched Gene 1, a Glycosylated Secretory Protein, Binds to FGFR and Mediates an Anti-stress Pathway to Protect Liver Development in Zebrafish.
Hu M, Bai Y, Zhang C, Liu F, Cui Z, Chen J, Peng J
PLoS genetics12(2)e1005881(2016 Feb)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Hypoxia-inducible factor 2 alpha is essential for hepatic outgrowth and functions via the regulation of leg1 transcription in the zebrafish embryo.
Lin TY, Chou CF, Chung HY, Chiang CY, Li CH, Wu JL, Lin HJ, Pai TW, Hu CH, Tzou WS
PloS one9(7)e101980(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Leg1 plays a unique role in protecting liver development under different stress conditions by serving as a secreted signaling molecule/modulator.
Title: Liver-Enriched Gene 1, a Glycosylated Secretory Protein, Binds to FGFR and Mediates an Anti-stress Pathway to Protect Liver Development in Zebrafish.

hif2-alpha-leg1 axis in liver development may have resulted from the adaptation of teleosts to their environment
Title: Hypoxia-inducible factor 2 alpha is essential for hepatic outgrowth and functions via the regulation of leg1 transcription in the zebrafish embryo.

Data suggest that leg1a (NP_001093526.1) and leg1b (NP_998368.1) expression are differentially regulated.
Title: liver-enriched gene 1a and 1b encode novel secretory proteins essential for normal liver development in zebrafish.

The following leg1.1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the leg1.1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35473	NM_001100056.2 Latest version!	Danio rerio liver-enriched gene 1, tandem duplicate 1 (leg1.1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

leg1.1 ( NM_001100056.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001093526.1 Danio rerio liver-enriched gene 1, tandem duplicate 1 (leg1.1), mRNA.

Price & Availability↑

CloneID	ODa35473
Clone ID Related Accession (Same CDS sequence)	NM_001100056.2
Accession Version	NM_001100056.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC796447 precursor
Comment	Comment: PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from BC155632.1. On Dec 11, 2007 this sequence version replaced NM_001100056.1. ##Evidence-Data-START## Transcript exon combination :: BC155632.1, EH495170.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGAGA TGGGTTTCCT GCGATCAGTG GCCGCTGTGC TCTTGTTGGC TGTGTTTAGC 
CACGCAGCAG TAGTGACTGA GAATGGACTG CCGATTCAAT GGGGAAAAGC TCCCAGTGAT 
CTCTCCCATC TGCCCATATC GGACACTGGG GTCCAGGTAA ACCCGTGGGA CTACTCCCAG 
AGGATGACCA TGTACAAAAT GCTGATCAAC GCTACTAATG CCTACATGAG CTCCATGGGC 
CCCGGGGAGC AGGAGAATCC GCTCTGGAGT CTTCCTCTGC AGCTCGGCTG GAAACTCAAA 
TCAGGCCGGC TGGCGGACCC GACCCTGGAC AGCAGCAGCA CATGTGGATC TGAAGCTTCA 
GATCCGGTCT GCATCTCTCC TCTGAGCTGG TTTGCATGTG TAAACTACTA CCTGTCTGTG 
CTGCCATTCC TGGCGGCAGT GGAGACTGGT GTGGTTAGCA GCGGAGGGCA TCAGGTGCTT 
ATTCAGGTCC CAGCAGAAGT GGCTCAGGAT TACTGCTCCA GCTACAGCGA CTGCTCCACC 
AAACACCCCA ACGCCATGGC CAAATGGCAC CTCTTTTTTC AGTCTCTGCG GCAGGTCAGC 
CAGTCCGAGG ACAGCGATTT TAACAAGAAG GACAGCATTC TGGGCCTGAT GTGGGCTGCC 
GAGGAGGAGT CTCTACAAAC CGCCTCTGGG GCTTGCACTG AAAGGCAAAA GCTGTACTCA 
TCTCCTGAGG TGAGCTTTCA GCAGAGCTGG CTGAACTCGG CAGCGTTTGT ATCGGCGGCT 
CATTTCCATG CAAACATTGA GCGCTCTGAG AAGTTTATGG CTCCTCTGCC CAGCCGAGTC 
CTGCAGGAGG CCGACAGTCC ACCCAACATC GCAGATCTGA GCACTGAAGA GAACCACACA 
CTCTACATCT TCGGCTGGAT GAACAGCGTC AACCAGCTGC TGGGCGGCTC TCTGGTGAAT 
TTGTGGCGTA AGGCCATGTG TTCGGCTCAG GCTCGAGAAA AAGGTCAGGC TCTTCTTCAC 
GACCTCATTC TGGACCCAAA GTTCCCCGGG TCCAGCCTTT GGTCCATTCT AAGCGAGATG 
TCCACCAGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093526.1
CDS	6..1100
Translation	MSEMGFLRSVAAVLLLAVFSHAAVVTENGLPIQWGKAPSDLSHLPISDTGVQVNPWDYSQRMTMYKMLINATNAYMSSMGPGEQENPLWSLPLQLGWKLKSGRLADPTLDSSSTCGSEASDPVCISPLSWFACVNYYLSVLPFLAAVETGVVSSGGHQVLIQVPAEVAQDYCSSYSDCSTKHPNAMAKWHLFFQSLRQVSQSEDSDFNKKDSILGLMWAAEEESLQTASGACTERQKLYSSPEVSFQQSWLNSAAFVSAAHFHANIERSEKFMAPLPSRVLQEADSPPNIADLSTEENHTLYIFGWMNSVNQLLGGSLVNLWRKAMCSAQAREKGQALLHDLILDPKFPGSSLWSILSEMSTSC

Target ORF information:

RefSeq Version	NM_001100056.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio liver-enriched gene 1, tandem duplicate 1 (leg1.1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100056.2

ORF Insert Sequence:

ATGTCTGAGA TGGGTTTCCT GCGATCAGTG GCCGCTGTGC TCTTGTTGGC TGTGTTTAGC 
CACGCAGCAG TAGTGACTGA GAATGGACTG CCGATTCAAT GGGGAAAAGC TCCCAGTGAT 
CTCTCCCATC TGCCCATATC GGACACTGGG GTCCAGGTAA ACCCGTGGGA CTACTCCCAG 
AGGATGACCA TGTACAAAAT GCTGATCAAC GCTACTAATG CCTACATGAG CTCCATGGGC 
CCCGGGGAGC AGGAGAATCC GCTCTGGAGT CTTCCTCTGC AGCTCGGCTG GAAACTCAAA 
TCAGGCCGGC TGGCGGACCC GACCCTGGAC AGCAGCAGCA CATGTGGATC TGAAGCTTCA 
GATCCGGTCT GCATCTCTCC TCTGAGCTGG TTTGCATGTG TAAACTACTA CCTGTCTGTG 
CTGCCATTCC TGGCGGCAGT GGAGACTGGT GTGGTTAGCA GCGGAGGGCA TCAGGTGCTT 
ATTCAGGTCC CAGCAGAAGT GGCTCAGGAT TACTGCTCCA GCTACAGCGA CTGCTCCACC 
AAACACCCCA ACGCCATGGC CAAATGGCAC CTCTTTTTTC AGTCTCTGCG GCAGGTCAGC 
CAGTCCGAGG ACAGCGATTT TAACAAGAAG GACAGCATTC TGGGCCTGAT GTGGGCTGCC 
GAGGAGGAGT CTCTACAAAC CGCCTCTGGG GCTTGCACTG AAAGGCAAAA GCTGTACTCA 
TCTCCTGAGG TGAGCTTTCA GCAGAGCTGG CTGAACTCGG CAGCGTTTGT ATCGGCGGCT 
CATTTCCATG CAAACATTGA GCGCTCTGAG AAGTTTATGG CTCCTCTGCC CAGCCGAGTC 
CTGCAGGAGG CCGACAGTCC ACCCAACATC GCAGATCTGA GCACTGAAGA GAACCACACA 
CTCTACATCT TCGGCTGGAT GAACAGCGTC AACCAGCTGC TGGGCGGCTC TCTGGTGAAT 
TTGTGGCGTA AGGCCATGTG TTCGGCTCAG GCTCGAGAAA AAGGTCAGGC TCTTCTTCAC 
GACCTCATTC TGGACCCAAA GTTCCCCGGG TCCAGCCTTT GGTCCATTCT AAGCGAGATG 
TCCACCAGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Liver-Enriched Gene 1, a Glycosylated Secretory Protein, Binds to FGFR and Mediates an Anti-stress Pathway to Protect Liver Development in Zebrafish.
PLoS genetics12(2)e1005881(2016 Feb)
Hu M,Bai Y,Zhang C,Liu F,Cui Z,Chen J,Peng J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Hypoxia-inducible factor 2 alpha is essential for hepatic outgrowth and functions via the regulation of leg1 transcription in the zebrafish embryo.
PloS one9(7)e101980(2014)
Lin TY,Chou CF,Chung HY,Chiang CY,Li CH,Wu JL,Lin HJ,Pai TW,Hu CH,Tzou WS