slc7a4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	slc7a4
Entrez Gene ID	796100
Full Name	solute carrier family 7, member 4
Synonyms	si:busm1-266f07.3,si:dz266f07.3
General protein information	Preferred Names solute carrier family 7, member 4 Names cationic amino acid transporter 4 solute carrier family 7 (cationic amino acid transporter, y+ system), member 4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017357381.1	XP_017212870.1	cationic amino acid transporter 4 isoform X1
NM_194427.2	NP_919408.2	cationic amino acid transporter 4

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SLC7A4	XP_424658.4
Drosophila melanogaster (fruit fly)	CG13248	NP_649219.1
Xenopus tropicalis (tropical clawed frog)	slc7a4	NP_001072820.1
Homo sapiens (human)	SLC7A4	NP_004164.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010567	XP_314539.2
Bos taurus (cattle)	SLC7A4	NP_001178971.1
Rattus norvegicus (Norway rat)	Slc7a4	NP_001100548.1
Danio rerio (zebrafish)	slc7a4	NP_919408.2
	SLC7A4	XP_001084016.1
Pan troglodytes (chimpanzee)	SLC7A4	XP_001168268.2
Canis lupus familiaris (dog)	SLC7A4	XP_005636673.1
Mus musculus (house mouse)	Slc7a4	NP_659101.2

Gene Ontology
Bibliography

Related articles in PubMed

Genetic Drivers of Kidney Defects in the DiGeorge Syndrome.
Lopez-Rivera E, Liu YP, Verbitsky M, Anderson BR, Capone VP, Otto EA, Yan Z, Mitrotti A, Martino J, Steers NJ, Fasel DA, Vukojevic K, Deng R, Racedo SE, Liu Q, Werth M, Westland R, Vivante A, Makar GS, Bodria M, Sampson MG, Gillies CE, Vega-Warner V, Maiorana M, Petrey DS, Honig B, Lozanovski VJ, Salomon R, Heidet L, Carpentier W, Gaillard D, Carrea A, Gesualdo L, Cusi D, Izzi C, Scolari F, van Wijk JA, Arapovic A, Saraga-Babic M, Saraga M, Kunac N, Samii A, McDonald-McGinn DM, Crowley TB, Zackai EH, Drozdz D, Miklaszewska M, Tkaczyk M, Sikora P, Szczepanska M, Mizerska-Wasiak M, Krzemien G, Szmigielska A, Zaniew M, Darlow JM, Puri P, Barton D, Casolari E, Furth SL, Warady BA, Gucev Z, Hakonarson H, Flogelova H, Tasic V, Latos-Bielenska A, Materna-Kiryluk A, Allegri L, Wong CS, Drummond IA, D'Agati V, Imamoto A, Barasch JM, Hildebrandt F, Kiryluk K, Lifton RP, Morrow BE, Jeanpierre C, Papaioannou VE, Ghiggeri GM, Gharavi AG, Katsanis N, Sanna-Cherchi S
The New England journal of medicine376(8)742-754(2017 02)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Comparative mapping of the 22q11.2 deletion region and the potential of simple model organisms.
Guna A, Butcher NJ, Bassett AS
Journal of neurodevelopmental disorders7(1)18(2015)

Evidence that the separation of Mhc class II from class I loci in the zebrafish, Danio rerio, occurred by translocation.
Kuroda N, Figueroa F, O'hUigin C, Klein J
Immunogenetics54(6)418-30(2002 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following slc7a4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slc7a4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa14436	NM_194427.2 Latest version!	Danio rerio solute carrier family 7 member 4 (slc7a4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa14436	XM_017357381.1 Latest version!	Danio rerio solute carrier family 7, member 4 (slc7a4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slc7a4 ( NM_194427.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_919408.2 Danio rerio solute carrier family 7 member 4 (slc7a4), mRNA.

Price & Availability↑

CloneID	ODa14436
Clone ID Related Accession (Same CDS sequence)	XM_017357381.1 , NM_194427.2
Accession Version	NM_194427.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	cationic amino acid transporter 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC134892.1. On or before Jan 24, 2008 this sequence version replaced XM_001333966.1, NM_194427.1. ##Evidence-Data-START## Transcript exon combination :: BC134892.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END##

ATGGCATCAT GCGTTTCCGG CTGTTCTCCA GTGATGAGGT TCTGTCAGAA GCTGAACAGG 
CTGAAGACCC TGGACGACGA TGTAATGGCA ACTTCACTGA AACGCTGTCT GAACACAGTG 
GACCTTGCTC TGCTTGGTGT AGGTGGAATG GTGGGTTCAG GCCTGTATGT TCTCACAGGA 
ACTGTTGCAA AAGACACAGC AGGACCCGCT GTAGTGATAT CTTTCCTTAT AGCTGGAGTA 
GCGTCACTGA TGGCAGCGCT GTGCTATGCT GAATTTGGCG CTCGTGTACC AAAGACAGGC 
TCTGCCTACA TGTTCACATA TGTTTCTGTT GGCGAAATCT GGGCATTCCT GATTGGCTGG 
AATGTCATTT TGGAGTATAT GATTGGCGGA GCGGCGGTAG CTCGTGCATG GAGTGGCTAT 
CTGGACTCTA TCTTTAATCA CCGCATCCAG AACTTCACAG AAAGTCATGT GATGCGCTGG 
GAAGTGCCCT TTCTTGCTCA TTACCCAGAC CTCCTAGCAG CTGCAATCCT CCTAGTGGCT 
TCCTTTTTCA TTTCATTTGG TGTTCGTGTC TCGTCTTGGC TTAACCACAT ATTTTCGGCC 
ATCAGTATGG TGGTCATTGT GTTTATTTTG GTGTTTGGCT TCATATTGGC CGATCCTGTC 
AATTGGAGCG CTCGGGAGGG AGGCTTTGCC CCGTTTGGGC TTTCGGGTAT CATGGCGGGA 
ACTGCAACAT GCTTCTATGC TTTTGTTGGC TTTGACGTAA TCGCAGCTTC CAGCGAGGAG 
GCCAGTAATC CTCAGAGGGC CATTCCTGCA GCAACTGCCA TCTCATTGGG TCTTGCGGCC 
ACAGCTTATG TCCTTGTGTC CACAGTTCTT ACATTGATGG TTCCATGGCA CACGCTTGAC 
CCAAACTCAG CTCTCTCGGA TGCGTTCTTC AGACGAGGTT ATAGCTGGGC TGGGTTTATT 
GTGGCTGTGG GATCCATATG TGCCATGAAC ACTGTGTTGC TCAGCAACCT TTTCTCTCTC 
CCGCGGATCG TTTACGCCAT GGCGGAGGAT GGCCTCTTCT TCTCTGTGTT TTCCCGTGTC 
AACCCTGTAA CCAAAGTCCC AGTGATCGCC ATTCTCGTTT TTGGCAGCCT TATGGCGATC 
CTTGCCCTCA TCTTTGATCT AGAAGCTCTG GTCCAGTTCC TCTCTATAGG CACTCTGCTT 
GCTTACACTT TTGTAGCAGC TAGTGTCATA GTGCTCCGCT TCCAACCGGA TAAAAGCAGC 
GGGAGTTCAG GAGCAGGTGG AGTCAAGACC GATGCTTCAT CCACACCTTC AGGAAACCAG 
TCAGGGCAGC AGATGGATCT ATCTGTGGCA GCAAATGAAG CCCAGACATT TGCGCAGGAT 
GGTGGAGAGC TGAAGGAGTA CGAGTCTTTC TCTGATAAAC TTCAACTGGT GGAGAGGCAA 
AAGGCAGGGA AGGAGAAACG GGCACCCGGG ATGCTGAAGG CTCGCTGGGA GCCATATTTA 
GGTCGATTGC TGGGCGACTG TGAGCCCGGT GAAGTGGTGG CGTTCTCAGT GCTTGCTCTG 
ATGGTCAGTG GAGTCTCTCT TAGTGCTGTG TTGGTGTTTG GGAATAACCA GCTGCATCTG 
CCTGTGTGGA GCTTCACCCT GCTTCTGGTC ATCTTCAGTG TCGTCTTCCT GCTCAGTCTG 
GCTCTCATCT CGGTCCATGA ACAGCGGCCC AACCTTAAGA CTTTTCAGGT TCCTCTGGTG 
CCATTTATTC CTGGCTTCAG CATCCTTCTG AATGTATTCC TCATGTTGAA GTTAAGTCCT 
CTCACCTGGA TTCGGTTCAC CGTCTGGGTC GCTGCAGGCC TCCTGGTATA TTTCGGGTAC 
GGCATCTGGC ACAGTAAGGA GGGTTTACGG GAACAGCAGG GGCAGGACGT GGCAGCGCGT 
TACGTGGTTC TTCCCAGCGG CAGCCTGGTG GAGACGGTCC AGAACGTCCA GCCTGAAGGA 
CACACTCACA GCACACACAC TTCAACCGTT GACAATCCAC CTGCCAGCAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919408.2
CDS	127..2160
Translation	MASCVSGCSPVMRFCQKLNRLKTLDDDVMATSLKRCLNTVDLALLGVGGMVGSGLYVLTGTVAKDTAGPAVVISFLIAGVASLMAALCYAEFGARVPKTGSAYMFTYVSVGEIWAFLIGWNVILEYMIGGAAVARAWSGYLDSIFNHRIQNFTESHVMRWEVPFLAHYPDLLAAAILLVASFFISFGVRVSSWLNHIFSAISMVVIVFILVFGFILADPVNWSAREGGFAPFGLSGIMAGTATCFYAFVGFDVIAASSEEASNPQRAIPAATAISLGLAATAYVLVSTVLTLMVPWHTLDPNSALSDAFFRRGYSWAGFIVAVGSICAMNTVLLSNLFSLPRIVYAMAEDGLFFSVFSRVNPVTKVPVIAILVFGSLMAILALIFDLEALVQFLSIGTLLAYTFVAASVIVLRFQPDKSSGSSGAGGVKTDASSTPSGNQSGQQMDLSVAANEAQTFAQDGGELKEYESFSDKLQLVERQKAGKEKRAPGMLKARWEPYLGRLLGDCEPGEVVAFSVLALMVSGVSLSAVLVFGNNQLHLPVWSFTLLLVIFSVVFLLSLALISVHEQRPNLKTFQVPLVPFIPGFSILLNVFLMLKLSPLTWIRFTVWVAAGLLVYFGYGIWHSKEGLREQQGQDVAARYVVLPSGSLVETVQNVQPEGHTHSTHTSTVDNPPASR

Target ORF information:

RefSeq Version	NM_194427.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 7 member 4 (slc7a4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194427.2

ORF Insert Sequence:

ATGGCATCAT GCGTTTCCGG CTGTTCTCCA GTGATGAGGT TCTGTCAGAA GCTGAACAGG 
CTGAAGACCC TGGACGACGA TGTAATGGCA ACTTCACTGA AACGCTGTCT GAACACAGTG 
GACCTTGCTC TGCTTGGTGT AGGTGGAATG GTGGGTTCAG GCCTGTATGT TCTCACAGGA 
ACTGTTGCAA AAGACACAGC AGGACCCGCT GTAGTGATAT CTTTCCTTAT AGCTGGAGTA 
GCGTCACTGA TGGCAGCGCT GTGCTATGCT GAATTTGGCG CTCGTGTACC AAAGACAGGC 
TCTGCCTACA TGTTCACATA TGTTTCTGTT GGCGAAATCT GGGCATTCCT GATTGGCTGG 
AATGTCATTT TGGAGTATAT GATTGGCGGA GCGGCGGTAG CTCGTGCATG GAGTGGCTAT 
CTGGACTCTA TCTTTAATCA CCGCATCCAG AACTTCACAG AAAGTCATGT GATGCGCTGG 
GAAGTGCCCT TTCTTGCTCA TTACCCAGAC CTCCTAGCAG CTGCAATCCT CCTAGTGGCT 
TCCTTTTTCA TTTCATTTGG TGTTCGTGTC TCGTCTTGGC TTAACCACAT ATTTTCGGCC 
ATCAGTATGG TGGTCATTGT GTTTATTTTG GTGTTTGGCT TCATATTGGC CGATCCTGTC 
AATTGGAGCG CTCGGGAGGG AGGCTTTGCC CCGTTTGGGC TTTCGGGTAT CATGGCGGGA 
ACTGCAACAT GCTTCTATGC TTTTGTTGGC TTTGACGTAA TCGCAGCTTC CAGCGAGGAG 
GCCAGTAATC CTCAGAGGGC CATTCCTGCA GCAACTGCCA TCTCATTGGG TCTTGCGGCC 
ACAGCTTATG TCCTTGTGTC CACAGTTCTT ACATTGATGG TTCCATGGCA CACGCTTGAC 
CCAAACTCAG CTCTCTCGGA TGCGTTCTTC AGACGAGGTT ATAGCTGGGC TGGGTTTATT 
GTGGCTGTGG GATCCATATG TGCCATGAAC ACTGTGTTGC TCAGCAACCT TTTCTCTCTC 
CCGCGGATCG TTTACGCCAT GGCGGAGGAT GGCCTCTTCT TCTCTGTGTT TTCCCGTGTC 
AACCCTGTAA CCAAAGTCCC AGTGATCGCC ATTCTCGTTT TTGGCAGCCT TATGGCGATC 
CTTGCCCTCA TCTTTGATCT AGAAGCTCTG GTCCAGTTCC TCTCTATAGG CACTCTGCTT 
GCTTACACTT TTGTAGCAGC TAGTGTCATA GTGCTCCGCT TCCAACCGGA TAAAAGCAGC 
GGGAGTTCAG GAGCAGGTGG AGTCAAGACC GATGCTTCAT CCACACCTTC AGGAAACCAG 
TCAGGGCAGC AGATGGATCT ATCTGTGGCA GCAAATGAAG CCCAGACATT TGCGCAGGAT 
GGTGGAGAGC TGAAGGAGTA CGAGTCTTTC TCTGATAAAC TTCAACTGGT GGAGAGGCAA 
AAGGCAGGGA AGGAGAAACG GGCACCCGGG ATGCTGAAGG CTCGCTGGGA GCCATATTTA 
GGTCGATTGC TGGGCGACTG TGAGCCCGGT GAAGTGGTGG CGTTCTCAGT GCTTGCTCTG 
ATGGTCAGTG GAGTCTCTCT TAGTGCTGTG TTGGTGTTTG GGAATAACCA GCTGCATCTG 
CCTGTGTGGA GCTTCACCCT GCTTCTGGTC ATCTTCAGTG TCGTCTTCCT GCTCAGTCTG 
GCTCTCATCT CGGTCCATGA ACAGCGGCCC AACCTTAAGA CTTTTCAGGT TCCTCTGGTG 
CCATTTATTC CTGGCTTCAG CATCCTTCTG AATGTATTCC TCATGTTGAA GTTAAGTCCT 
CTCACCTGGA TTCGGTTCAC CGTCTGGGTC GCTGCAGGCC TCCTGGTATA TTTCGGGTAC 
GGCATCTGGC ACAGTAAGGA GGGTTTACGG GAACAGCAGG GGCAGGACGT GGCAGCGCGT 
TACGTGGTTC TTCCCAGCGG CAGCCTGGTG GAGACGGTCC AGAACGTCCA GCCTGAAGGA 
CACACTCACA GCACACACAC TTCAACCGTT GACAATCCAC CTGCCAGCAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc7a4 ( XM_017357381.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212870.1 Danio rerio solute carrier family 7, member 4 (slc7a4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa14436
Clone ID Related Accession (Same CDS sequence)	XM_017357381.1 , NM_194427.2
Accession Version	XM_017357381.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	cationic amino acid transporter 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCAT GCGTTTCCGG CTGTTCTCCA GTGATGAGGT TCTGTCAGAA GCTGAACAGG 
CTGAAGACCC TGGACGACGA TGTAATGGCA ACTTCACTGA AACGCTGTCT GAACACAGTG 
GACCTTGCTC TGCTTGGTGT AGGTGGAATG GTGGGTTCAG GCCTGTATGT TCTCACAGGA 
ACTGTTGCAA AAGACACAGC AGGACCCGCT GTAGTGATAT CTTTCCTTAT AGCTGGAGTA 
GCGTCACTGA TGGCAGCGCT GTGCTATGCT GAATTTGGCG CTCGTGTACC AAAGACAGGC 
TCTGCCTACA TGTTCACATA TGTTTCTGTT GGCGAAATCT GGGCATTCCT GATTGGCTGG 
AATGTCATTT TGGAGTATAT GATTGGCGGA GCGGCGGTAG CTCGTGCATG GAGTGGCTAT 
CTGGACTCTA TCTTTAATCA CCGCATCCAG AACTTCACAG AAAGTCATGT GATGCGCTGG 
GAAGTGCCCT TTCTTGCTCA TTACCCAGAC CTCCTAGCAG CTGCAATCCT CCTAGTGGCT 
TCCTTTTTCA TTTCATTTGG TGTTCGTGTC TCGTCTTGGC TTAACCACAT ATTTTCGGCC 
ATCAGTATGG TGGTCATTGT GTTTATTTTG GTGTTTGGCT TCATATTGGC CGATCCTGTC 
AATTGGAGCG CTCGGGAGGG AGGCTTTGCC CCGTTTGGGC TTTCGGGTAT CATGGCGGGA 
ACTGCAACAT GCTTCTATGC TTTTGTTGGC TTTGACGTAA TCGCAGCTTC CAGCGAGGAG 
GCCAGTAATC CTCAGAGGGC CATTCCTGCA GCAACTGCCA TCTCATTGGG TCTTGCGGCC 
ACAGCTTATG TCCTTGTGTC CACAGTTCTT ACATTGATGG TTCCATGGCA CACGCTTGAC 
CCAAACTCAG CTCTCTCGGA TGCGTTCTTC AGACGAGGTT ATAGCTGGGC TGGGTTTATT 
GTGGCTGTGG GATCCATATG TGCCATGAAC ACTGTGTTGC TCAGCAACCT TTTCTCTCTC 
CCGCGGATCG TTTACGCCAT GGCGGAGGAT GGCCTCTTCT TCTCTGTGTT TTCCCGTGTC 
AACCCTGTAA CCAAAGTCCC AGTGATCGCC ATTCTCGTTT TTGGCAGCCT TATGGCGATC 
CTTGCCCTCA TCTTTGATCT AGAAGCTCTG GTCCAGTTCC TCTCTATAGG CACTCTGCTT 
GCTTACACTT TTGTAGCAGC TAGTGTCATA GTGCTCCGCT TCCAACCGGA TAAAAGCAGC 
GGGAGTTCAG GAGCAGGTGG AGTCAAGACC GATGCTTCAT CCACACCTTC AGGAAACCAG 
TCAGGGCAGC AGATGGATCT ATCTGTGGCA GCAAATGAAG CCCAGACATT TGCGCAGGAT 
GGTGGAGAGC TGAAGGAGTA CGAGTCTTTC TCTGATAAAC TTCAACTGGT GGAGAGGCAA 
AAGGCAGGGA AGGAGAAACG GGCACCCGGG ATGCTGAAGG CTCGCTGGGA GCCATATTTA 
GGTCGATTGC TGGGCGACTG TGAGCCCGGT GAAGTGGTGG CGTTCTCAGT GCTTGCTCTG 
ATGGTCAGTG GAGTCTCTCT TAGTGCTGTG TTGGTGTTTG GGAATAACCA GCTGCATCTG 
CCTGTGTGGA GCTTCACCCT GCTTCTGGTC ATCTTCAGTG TCGTCTTCCT GCTCAGTCTG 
GCTCTCATCT CGGTCCATGA ACAGCGGCCC AACCTTAAGA CTTTTCAGGT TCCTCTGGTG 
CCATTTATTC CTGGCTTCAG CATCCTTCTG AATGTATTCC TCATGTTGAA GTTAAGTCCT 
CTCACCTGGA TTCGGTTCAC CGTCTGGGTC GCTGCAGGCC TCCTGGTATA TTTCGGGTAC 
GGCATCTGGC ACAGTAAGGA GGGTTTACGG GAACAGCAGG GGCAGGACGT GGCAGCGCGT 
TACGTGGTTC TTCCCAGCGG CAGCCTGGTG GAGACGGTCC AGAACGTCCA GCCTGAAGGA 
CACACTCACA GCACACACAC TTCAACCGTT GACAATCCAC CTGCCAGCAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212870.1
CDS	181..2214
Translation	MASCVSGCSPVMRFCQKLNRLKTLDDDVMATSLKRCLNTVDLALLGVGGMVGSGLYVLTGTVAKDTAGPAVVISFLIAGVASLMAALCYAEFGARVPKTGSAYMFTYVSVGEIWAFLIGWNVILEYMIGGAAVARAWSGYLDSIFNHRIQNFTESHVMRWEVPFLAHYPDLLAAAILLVASFFISFGVRVSSWLNHIFSAISMVVIVFILVFGFILADPVNWSAREGGFAPFGLSGIMAGTATCFYAFVGFDVIAASSEEASNPQRAIPAATAISLGLAATAYVLVSTVLTLMVPWHTLDPNSALSDAFFRRGYSWAGFIVAVGSICAMNTVLLSNLFSLPRIVYAMAEDGLFFSVFSRVNPVTKVPVIAILVFGSLMAILALIFDLEALVQFLSIGTLLAYTFVAASVIVLRFQPDKSSGSSGAGGVKTDASSTPSGNQSGQQMDLSVAANEAQTFAQDGGELKEYESFSDKLQLVERQKAGKEKRAPGMLKARWEPYLGRLLGDCEPGEVVAFSVLALMVSGVSLSAVLVFGNNQLHLPVWSFTLLLVIFSVVFLLSLALISVHEQRPNLKTFQVPLVPFIPGFSILLNVFLMLKLSPLTWIRFTVWVAAGLLVYFGYGIWHSKEGLREQQGQDVAARYVVLPSGSLVETVQNVQPEGHTHSTHTSTVDNPPASR

Target ORF information:

RefSeq Version	XM_017357381.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio solute carrier family 7, member 4 (slc7a4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357381.1

ORF Insert Sequence:

ATGGCATCAT GCGTTTCCGG CTGTTCTCCA GTGATGAGGT TCTGTCAGAA GCTGAACAGG 
CTGAAGACCC TGGACGACGA TGTAATGGCA ACTTCACTGA AACGCTGTCT GAACACAGTG 
GACCTTGCTC TGCTTGGTGT AGGTGGAATG GTGGGTTCAG GCCTGTATGT TCTCACAGGA 
ACTGTTGCAA AAGACACAGC AGGACCCGCT GTAGTGATAT CTTTCCTTAT AGCTGGAGTA 
GCGTCACTGA TGGCAGCGCT GTGCTATGCT GAATTTGGCG CTCGTGTACC AAAGACAGGC 
TCTGCCTACA TGTTCACATA TGTTTCTGTT GGCGAAATCT GGGCATTCCT GATTGGCTGG 
AATGTCATTT TGGAGTATAT GATTGGCGGA GCGGCGGTAG CTCGTGCATG GAGTGGCTAT 
CTGGACTCTA TCTTTAATCA CCGCATCCAG AACTTCACAG AAAGTCATGT GATGCGCTGG 
GAAGTGCCCT TTCTTGCTCA TTACCCAGAC CTCCTAGCAG CTGCAATCCT CCTAGTGGCT 
TCCTTTTTCA TTTCATTTGG TGTTCGTGTC TCGTCTTGGC TTAACCACAT ATTTTCGGCC 
ATCAGTATGG TGGTCATTGT GTTTATTTTG GTGTTTGGCT TCATATTGGC CGATCCTGTC 
AATTGGAGCG CTCGGGAGGG AGGCTTTGCC CCGTTTGGGC TTTCGGGTAT CATGGCGGGA 
ACTGCAACAT GCTTCTATGC TTTTGTTGGC TTTGACGTAA TCGCAGCTTC CAGCGAGGAG 
GCCAGTAATC CTCAGAGGGC CATTCCTGCA GCAACTGCCA TCTCATTGGG TCTTGCGGCC 
ACAGCTTATG TCCTTGTGTC CACAGTTCTT ACATTGATGG TTCCATGGCA CACGCTTGAC 
CCAAACTCAG CTCTCTCGGA TGCGTTCTTC AGACGAGGTT ATAGCTGGGC TGGGTTTATT 
GTGGCTGTGG GATCCATATG TGCCATGAAC ACTGTGTTGC TCAGCAACCT TTTCTCTCTC 
CCGCGGATCG TTTACGCCAT GGCGGAGGAT GGCCTCTTCT TCTCTGTGTT TTCCCGTGTC 
AACCCTGTAA CCAAAGTCCC AGTGATCGCC ATTCTCGTTT TTGGCAGCCT TATGGCGATC 
CTTGCCCTCA TCTTTGATCT AGAAGCTCTG GTCCAGTTCC TCTCTATAGG CACTCTGCTT 
GCTTACACTT TTGTAGCAGC TAGTGTCATA GTGCTCCGCT TCCAACCGGA TAAAAGCAGC 
GGGAGTTCAG GAGCAGGTGG AGTCAAGACC GATGCTTCAT CCACACCTTC AGGAAACCAG 
TCAGGGCAGC AGATGGATCT ATCTGTGGCA GCAAATGAAG CCCAGACATT TGCGCAGGAT 
GGTGGAGAGC TGAAGGAGTA CGAGTCTTTC TCTGATAAAC TTCAACTGGT GGAGAGGCAA 
AAGGCAGGGA AGGAGAAACG GGCACCCGGG ATGCTGAAGG CTCGCTGGGA GCCATATTTA 
GGTCGATTGC TGGGCGACTG TGAGCCCGGT GAAGTGGTGG CGTTCTCAGT GCTTGCTCTG 
ATGGTCAGTG GAGTCTCTCT TAGTGCTGTG TTGGTGTTTG GGAATAACCA GCTGCATCTG 
CCTGTGTGGA GCTTCACCCT GCTTCTGGTC ATCTTCAGTG TCGTCTTCCT GCTCAGTCTG 
GCTCTCATCT CGGTCCATGA ACAGCGGCCC AACCTTAAGA CTTTTCAGGT TCCTCTGGTG 
CCATTTATTC CTGGCTTCAG CATCCTTCTG AATGTATTCC TCATGTTGAA GTTAAGTCCT 
CTCACCTGGA TTCGGTTCAC CGTCTGGGTC GCTGCAGGCC TCCTGGTATA TTTCGGGTAC 
GGCATCTGGC ACAGTAAGGA GGGTTTACGG GAACAGCAGG GGCAGGACGT GGCAGCGCGT 
TACGTGGTTC TTCCCAGCGG CAGCCTGGTG GAGACGGTCC AGAACGTCCA GCCTGAAGGA 
CACACTCACA GCACACACAC TTCAACCGTT GACAATCCAC CTGCCAGCAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic Drivers of Kidney Defects in the DiGeorge Syndrome.
The New England journal of medicine376(8)742-754(2017 02)
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