ccl25b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ccl25b
Entrez Gene ID	795788
Full Name	chemokine (C-C motif) ligand 25b
General protein information	Preferred Names chemokine (C-C motif) ligand 25b Names chemokine CCL-C11a CCL-chr11a Dr11_WGA839_1_159228 ccl21 dr-chr11-CCL21-36.7%-DN
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001200035.1	NP_001186964.1	chemokine CCL-C11a precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chd7 Is Critical for Early T-Cell Development and Thymus Organogenesis in Zebrafish.
Liu ZZ, Wang ZL, Choi TI, Huang WT, Wang HT, Han YY, Zhu LY, Kim HT, Choi JH, Lee JS, Kim HG, Zhao J, Chen Y, Lu Z, Tian XL, Pan BX, Li BM, Kim CH, Xu H
The American journal of pathology(2018 Jan)

The Vascular Niche Regulates Hematopoietic Stem and Progenitor Cell Lodgment and Expansion via klf6a-ccl25b.
Xue Y, Lv J, Zhang C, Wang L, Ma D, Liu F
Developmental cell42(4)349-362.e4(2017 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study identified and cloned the zebrafish orthologues of CCL21 and CCL25; CCL21 was expressed in the craniofacial region, pharynx, and blood vessels in embryos. In adult fish, CCL21 transcripts were located in the kidney, spinal cord, and blood cells.
Title: Cloning, expression and characterization of CCL21 and CCL25 chemokines in zebrafish.

The following ccl25b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ccl25b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37118	NM_001200035.1 Latest version!	Danio rerio chemokine (C-C motif) ligand 25b (ccl25b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ccl25b ( NM_001200035.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001186964.1 Danio rerio chemokine (C-C motif) ligand 25b (ccl25b), mRNA.

Price & Availability↑

CloneID	ODa37118
Clone ID Related Accession (Same CDS sequence)	NM_001200035.1
Accession Version	NM_001200035.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-05
Organism	Danio rerio(zebrafish)
Product	chemokine CCL-C11a precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH435143.1, BC133887.1 and EH555075.1. On Jan 14, 2011 this sequence version replaced XR_082624.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EH497103.1, DT070078.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAAACGC AGAGTTCAAC CATGAAGTTT CAAATTCTGG CTTTGCTCCT GCTGTTGGCT 
TGCATGTATC CCAGCATCGC ACAAGGTTAC TATGAAAACT GTTGTTTGAA ATATGTCACA 
GGCATCAAGA AAAACATGAG AAGAAATATT ATGAGTTACA GAGTGCAGCT AACAGACGGA 
GGATGCAACA TTCCCGCTGT TGTTTTCAAG ATGAGGCTCA AAAAACAGTT AAAACCAAAG 
TCAGTCTGCG CCGATCCAAG AAGTGACTGG GTCCAGGCGA TAATAAAGGA GCTGGATGAG 
AAAAACAAAA GAGCTATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186964.1
CDS	70..390
Translation	METQSSTMKFQILALLLLLACMYPSIAQGYYENCCLKYVTGIKKNMRRNIMSYRVQLTDGGCNIPAVVFKMRLKKQLKPKSVCADPRSDWVQAIIKELDEKNKRAM

Target ORF information:

RefSeq Version	NM_001200035.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chemokine (C-C motif) ligand 25b (ccl25b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001200035.1

ORF Insert Sequence:

ATGGAAACGC AGAGTTCAAC CATGAAGTTT CAAATTCTGG CTTTGCTCCT GCTGTTGGCT 
TGCATGTATC CCAGCATCGC ACAAGGTTAC TATGAAAACT GTTGTTTGAA ATATGTCACA 
GGCATCAAGA AAAACATGAG AAGAAATATT ATGAGTTACA GAGTGCAGCT AACAGACGGA 
GGATGCAACA TTCCCGCTGT TGTTTTCAAG ATGAGGCTCA AAAAACAGTT AAAACCAAAG 
TCAGTCTGCG CCGATCCAAG AAGTGACTGG GTCCAGGCGA TAATAAAGGA GCTGGATGAG 
AAAAACAAAA GAGCTATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chd7 Is Critical for Early T-Cell Development and Thymus Organogenesis in Zebrafish.
The American journal of pathology(2018 Jan)
Liu ZZ,Wang ZL,Choi TI,Huang WT,Wang HT,Han YY,Zhu LY,Kim HT,Choi JH,Lee JS,Kim HG,Zhao J,Chen Y,Lu Z,Tian XL,Pan BX,Li BM,Kim CH,Xu H

The Vascular Niche Regulates Hematopoietic Stem and Progenitor Cell Lodgment and Expansion via klf6a-ccl25b.
Developmental cell42(4)349-362.e4(2017 08)
Xue Y,Lv J,Zhang C,Wang L,Ma D,Liu F