rit1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rit1
Entrez Gene ID	793942
Full Name	Ras-like without CAAX 1
Synonyms	si:ch211-93a2.3
General protein information	Preferred Names Ras-like without CAAX 1 Names GTP-binding protein Rit1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021468142.1	XP_021323817.1	GTP-binding protein Rit1 isoform X1
NM_001128781.1	NP_001122253.1	GTP-binding protein Rit1

Pathways from BioSystems

Homologies

	RIT1	XP_001116270.1
Bos taurus (cattle)	RIT1	NP_001091547.1
Pan troglodytes (chimpanzee)	RIT1	XP_513868.4
Canis lupus familiaris (dog)	RIT1	XP_537249.2
Mus musculus (house mouse)	Rit1	NP_033095.1
Gallus gallus (chicken)	RIT1	NP_001026498.2
Danio rerio (zebrafish)	rit1	NP_001122253.1
Xenopus tropicalis (tropical clawed frog)	LOC100486360	XP_002944039.2
Homo sapiens (human)	RIT1	NP_001243750.1
Rattus norvegicus (Norway rat)	Rit1	NP_001102655.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Gain-of-function mutations in RIT1 cause Noonan syndrome, a RAS/MAPK pathway syndrome.
Aoki Y, Niihori T, Banjo T, Okamoto N, Mizuno S, Kurosawa K, Ogata T, Takada F, Yano M, Ando T, Hoshika T, Barnett C, Ohashi H, Kawame H, Hasegawa T, Okutani T, Nagashima T, Hasegawa S, Funayama R, Nagashima T, Nakayama K, Inoue S, Watanabe Y, Ogura T, Matsubara Y
American journal of human genetics93(1)173-80(2013 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rit1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rit1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35728	NM_001128781.1 Latest version!	Danio rerio Ras-like without CAAX 1 (rit1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa150126	XM_021468142.1 Latest version!	Danio rerio Ras-like without CAAX 1 (rit1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rit1 ( NM_001128781.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001122253.1 Danio rerio Ras-like without CAAX 1 (rit1), mRNA.

Price & Availability↑

CloneID	ODa35728
Clone ID Related Accession (Same CDS sequence)	NM_001128781.1
Accession Version	NM_001128781.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	GTP-binding protein Rit1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC163608.1. On Jul 31, 2008 this sequence version replaced XM_001332031.2. ##Evidence-Data-START## Transcript exon combination :: BC163607.1, GO942035.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGAGTCCT CGCGGAGCAC AGTGGGTCAC TCCCGCGAGT ACAAACTGGT GATGTTAGGA 
GAGGGAGGTG TGGGAAAAAG TGCCATCATC ATGCAGTTTA TAAGTCACAG GTTTCCAGAG 
GACCATGACC CTACCATTGA GGATGCCTAT AAAACACAGA TCCGCATTGA CGATGAGCCA 
GCAAACCTGG ACATCTTGGA CACAGCGGGA CAGGCGGAGT TTACAGCAAT GCGTGACCAG 
TATATGCGAG CAGGTGAAGG TTTCATCATC TCATACTCCA TCACTGACCG CCGCAGTTTC 
CAGGAGGCTC GTCACTTTAA GCAGCTGATT TACCGTGTAC GACGCACCGT GGACACACCT 
GTCGTGTTGG TGGGCAACAA GTCTGACCTG GTACATCTCA GACAGGTTTC CGTAGAAGAA 
GGCAAACAGC TGGCGAGAGA GTTCCAGTGC CCGTTTTTTG AGACTTCGGC AGCGTTTCGT 
TATTACATCG ATGAGGTTTT TGCAGCACTG GTGCGTCAGA TCCGCCAACA TGAGGCCGAA 
ATGGTGCGGG ACAGCGAGAG GAAAACCCGA CGCAGCCATT CCTTCTGGAG TCGCCTTAAA 
GCCCCCTTCC ATAGGAAACA GCAGTCCGAG CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122253.1
CDS	56..691
Translation	MESSRSTVGHSREYKLVMLGEGGVGKSAIIMQFISHRFPEDHDPTIEDAYKTQIRIDDEPANLDILDTAGQAEFTAMRDQYMRAGEGFIISYSITDRRSFQEARHFKQLIYRVRRTVDTPVVLVGNKSDLVHLRQVSVEEGKQLAREFQCPFFETSAAFRYYIDEVFAALVRQIRQHEAEMVRDSERKTRRSHSFWSRLKAPFHRKQQSEH

Target ORF information:

RefSeq Version	NM_001128781.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ras-like without CAAX 1 (rit1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128781.1

ORF Insert Sequence:

ATGGAGTCCT CGCGGAGCAC AGTGGGTCAC TCCCGCGAGT ACAAACTGGT GATGTTAGGA 
GAGGGAGGTG TGGGAAAAAG TGCCATCATC ATGCAGTTTA TAAGTCACAG GTTTCCAGAG 
GACCATGACC CTACCATTGA GGATGCCTAT AAAACACAGA TCCGCATTGA CGATGAGCCA 
GCAAACCTGG ACATCTTGGA CACAGCGGGA CAGGCGGAGT TTACAGCAAT GCGTGACCAG 
TATATGCGAG CAGGTGAAGG TTTCATCATC TCATACTCCA TCACTGACCG CCGCAGTTTC 
CAGGAGGCTC GTCACTTTAA GCAGCTGATT TACCGTGTAC GACGCACCGT GGACACACCT 
GTCGTGTTGG TGGGCAACAA GTCTGACCTG GTACATCTCA GACAGGTTTC CGTAGAAGAA 
GGCAAACAGC TGGCGAGAGA GTTCCAGTGC CCGTTTTTTG AGACTTCGGC AGCGTTTCGT 
TATTACATCG ATGAGGTTTT TGCAGCACTG GTGCGTCAGA TCCGCCAACA TGAGGCCGAA 
ATGGTGCGGG ACAGCGAGAG GAAAACCCGA CGCAGCCATT CCTTCTGGAG TCGCCTTAAA 
GCCCCCTTCC ATAGGAAACA GCAGTCCGAG CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rit1 ( XM_021468142.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323817.1 Danio rerio Ras-like without CAAX 1 (rit1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa150126
Clone ID Related Accession (Same CDS sequence)	XM_021468142.1
Accession Version	XM_021468142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	GTP-binding protein Rit1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTTA TAAGTCACAG GTTTCCAGAG GACCATGACC CTACCATTGA GGATGCCTAT 
AAAACACAGA TCCGCATTGA CGATGAGCCA GCAAACCTGG ACATCTTGGA CACAGCGGGA 
CAGGCGGAGT TTACAGCAAT GCGTGACCAG TATATGCGAG CAGGTGAAGG TTTCATCATC 
TCATACTCCA TCACTGACCG CCGCAGTTTC CAGGAGGCTC GTCACTTTAA GCAGCTGATT 
TACCGTGTAC GACGCACCGT GGACACACCT GTCGTGTTGG TGGGCAACAA GTCTGACCTG 
GTACATCTCA GACAGGTTTC CGTAGAAGAA GGCAAACAGC TGGCGAGAGA GTTCCAGTGC 
CCGTTTTTTG AGACTTCGGC AGCGTTTCGT TATTACATCG ATGAGGTTTT TGCAGCACTG 
GTGCGTCAGA TCCGCCAACA TGAGGCCGAA ATGGTGCGGG ACAGCGAGAG GAAAACCCGA 
CGCAGCCATT CCTTCTGGAG TCGCCTTAAA GCCCCCTTCC ATAGGAAACA GCAGTCCGAG 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323817.1
CDS	53..598
Translation	MQFISHRFPEDHDPTIEDAYKTQIRIDDEPANLDILDTAGQAEFTAMRDQYMRAGEGFIISYSITDRRSFQEARHFKQLIYRVRRTVDTPVVLVGNKSDLVHLRQVSVEEGKQLAREFQCPFFETSAAFRYYIDEVFAALVRQIRQHEAEMVRDSERKTRRSHSFWSRLKAPFHRKQQSEH

Target ORF information:

RefSeq Version	XM_021468142.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ras-like without CAAX 1 (rit1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468142.1

ORF Insert Sequence:

ATGCAGTTTA TAAGTCACAG GTTTCCAGAG GACCATGACC CTACCATTGA GGATGCCTAT 
AAAACACAGA TCCGCATTGA CGATGAGCCA GCAAACCTGG ACATCTTGGA CACAGCGGGA 
CAGGCGGAGT TTACAGCAAT GCGTGACCAG TATATGCGAG CAGGTGAAGG TTTCATCATC 
TCATACTCCA TCACTGACCG CCGCAGTTTC CAGGAGGCTC GTCACTTTAA GCAGCTGATT 
TACCGTGTAC GACGCACCGT GGACACACCT GTCGTGTTGG TGGGCAACAA GTCTGACCTG 
GTACATCTCA GACAGGTTTC CGTAGAAGAA GGCAAACAGC TGGCGAGAGA GTTCCAGTGC 
CCGTTTTTTG AGACTTCGGC AGCGTTTCGT TATTACATCG ATGAGGTTTT TGCAGCACTG 
GTGCGTCAGA TCCGCCAACA TGAGGCCGAA ATGGTGCGGG ACAGCGAGAG GAAAACCCGA 
CGCAGCCATT CCTTCTGGAG TCGCCTTAAA GCCCCCTTCC ATAGGAAACA GCAGTCCGAG 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Gain-of-function mutations in RIT1 cause Noonan syndrome, a RAS/MAPK pathway syndrome.
American journal of human genetics93(1)173-80(2013 Jul)
Aoki Y,Niihori T,Banjo T,Okamoto N,Mizuno S,Kurosawa K,Ogata T,Takada F,Yano M,Ando T,Hoshika T,Barnett C,Ohashi H,Kawame H,Hasegawa T,Okutani T,Nagashima T,Hasegawa S,Funayama R,Nagashima T,Nakayama K,Inoue S,Watanabe Y,Ogura T,Matsubara Y

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rit1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

オンラインフォーム