rasal2 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_001332652.8	XP_001332688.5	ras GTPase-activating protein nGAP isoform X3
XM_005171322.4	XP_005171379.1	ras GTPase-activating protein nGAP isoform X2
XM_005171324.4	XP_005171381.1	ras GTPase-activating protein nGAP isoform X4
XM_009298648.2	XP_009296923.1	ras GTPase-activating protein nGAP isoform X6
XM_005171325.4	XP_005171382.1	ras GTPase-activating protein nGAP isoform X5
XM_005171321.3	XP_005171378.1	ras GTPase-activating protein nGAP isoform X1
XM_005171322.3	XP_005171379.1	ras GTPase-activating protein nGAP isoform X2
XM_001332652.7	XP_001332688.5	ras GTPase-activating protein nGAP isoform X3
XM_005171324.3	XP_005171381.1	ras GTPase-activating protein nGAP isoform X4
XM_005171325.3	XP_005171382.1	ras GTPase-activating protein nGAP isoform X5
XM_005171321.4	XP_005171378.1	ras GTPase-activating protein nGAP isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa07240	XM_005171324.4 Latest version!	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa07241	XM_005171325.4 Latest version!	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa46832	XM_009298648.2 Latest version!	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa07236	XM_005171321.4 Latest version!	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa07237	XM_005171322.4 Latest version!	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa07239	XM_001332652.8 Latest version!	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa07237	XM_005171322.3	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa07239	XM_001332652.7	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa07240	XM_005171324.3	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa07241	XM_005171325.3	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa07236	XM_005171321.3	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

rasal2 ( XM_005171324.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171381.1 Danio rerio RAS protein activator like 2 (rasal2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa07240
Clone ID Related Accession (Same CDS sequence)	XM_005171324.4 , XM_005171324.3
Accession Version	XM_005171324.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras GTPase-activating protein nGAP isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171324.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAACA GCTGTGATAG AGCGGAAGCG TCCGCGGAGC GCTCTCCCAG ACGGAGGAGC 
ATATCAGGGC TAGGCAGCTC AGAGAAGAAC ATTTCCATCG ATGGCCCCAA CTCATCTCCC 
TTCAAAGTGC CTGGTTTTTT AAGTAAACGA CTGAAAGGAT CCATCAAAAG GACGAAGAGC 
CAGACTAAGC TGGACCGCAA CACAAGTTTC AGACTCCCAT CGCTGCGACC AACAGAAAAT 
GACAGGTCAC GAGGCCTGCC CAAATTGAAG GAGTCCTGTT CCCATGAGTC TTTGCTGAGT 
CCTGGCAGCG CTGTTGAAGC TCTGGATCTG AGTATGGAGG AGGATGTCTA CATCAAACCT 
TTACACAGCA GCATCCTAGG ACAAGATTTC TGCTTTGAGG TGGCGTACTC AGGTGGAAGT 
AAGTGCTTCA GCTGTTCCTC TGCTGCTGAA CGGGACAAAT GGATGGAGAA CCTCAAAAGA 
ACTGTGCAAC CTAATAAGGA TAATTGCCGG CGGGCGGAAA ACGTCCTCCG CCTGTGGATC 
ATTGAGGCAA AAGACCTGCC ACCAAAAAAG AAATATTTCT GTGAGCTGTG TCTGGATGAC 
ATGCTGTACG CTCGTACCAC CAGCAAAACC CGCTCCGACT GCCTGTTCTG GGGGGAACAC 
TTTGAGTTTT CAGGCCTCCC GTCCATGAAA AGCATCACCA TACACATCTA CCGGGATGTG 
GACAAGAAAA AGAAAAAGGA CAAAAACAAC TATGTTGGCC TGGTGAACAT CCCTGTGCAT 
GGGGTGATGG GTAGGCAGTT TGTGGAGAAG TGGTATCCAG TCAGCACTCC CACCACCAGC 
AAAGCCAAAG GTGGAGGCCC CTCCATCCGC ATCAAATCCC GCTTTCAGAC CATCTCAATT 
CTGCCCATGG AGCAGTACAA GGAGTTTGCG GAGTTTGTTA CCAATAACTA TACCATGTTG 
TGCTCTGTGT TGGAGCCGGT CATAAGCGTG AAGAACAAAG AGGAGATGGC CAGCGCTCTG 
GTGCACATTC TGCAGAGCAC GGGACGGGCA AAGGACTTCC TAACGGATTT GGTGATGTCA 
GAAGTGGATC GCTGTGCTGA CCATGATGTG CTGATCTTTA GAGAAAACAC ACTAGCCACC 
AAAGCCATTG AAGAATACCT CAAACTGGTG GGACAGAAGT ACCTACATGA TGCACTAGGG 
GAGTTTATTA AAGCCCTGTA CGAGTCGGAT GAAAATTGTG AAGTAGACCA ATCAAAGTGC 
ACCAGCAACG AGCTTCCAGA GCATCAGAGT AACCTGAAGA TGTGCTGCGA ACTGGCCTTC 
TGCAAGATCA TCAACTCTTA TTGTGTTTTT CCACGTGAAC TGAAGGAGGT CTTTGCATCA 
TGGAAGCAGC AGTGTCACGC TCGAGGGAAG CAGGACATCA GTCAGCGTCT TATCAGCGCA 
TCGCTCTTCC TGCGCTTTCT GTGTCCTGCC ATCATGTCCC CGTCACTCTT TGGTCTCATG 
CAGGAATATC CAGATGACCG TACTTCTCGA ACACTCACCC TCATTGCCAA AGTCATTCAG 
AATCTTGCTA ACTTTGCCAA ATTCGGGAAC AAAGAGGAGT ACATGGCTTT CATGAATGAC 
TTTCTAGAGC ACGAGTGGGC TGGTATGACC CGTTTTCTGC TTGAGATCTC GAATCTTGAG 
ACAATCTCCA ACACCCCGGG CTTTGAGGGC TACATTGACC TGGGCCGGGA GCTGTCAGTG 
CTTCACTCGC TGCTCTGGGA GGTGGTGTCT CAGCTGGACA AGGGTGACAA TTCCTTCCTG 
CAGGCGACGG TGGCCAAACT GGGGCCCCTG CCACGTATCC TTGGCGATAT CACCCGCTCG 
CTGTCCAGCC CTACTCCCAT CCAGCAGCAG CTCAGGCGCT TCCAGGACCA CAGCTCTGCT 
CATGACATCA GTGGAAGTGT GTCCTCAGGA CTGCAGAGAA TATTTGAAGA CCCAGCAGAC 
AGTGAAATGA GGAGCATCAA ATCACCTGTC CAAGAGCACA TGGAGGCTTT AGTCAGAGGA 
AAGCATCCTT TACTGGGTCA ACAGTCGTCC GCACACAGCA TGAGCTTCTC CGACAAAGAG 
GAAAGGGACA GTCCGCTCCC GAATGGACGC AGTATATCTT TAATGGACTT GCAGGACTCT 
TACCTTGCTC AGGGGCATCC AGGTCCCAAC TCGCTCAATG AAGCCTCATC TAGGTTAGGC 
AGAGTAGGCT CTCAGGCCTC CATCGGCCCT GTCCCTCCTC CACACCTCCA CCAGCCTCCA 
GTCCACCCGA AAGTTCCACA GCTGAGGGAT AACTTACCCC AGAGTGCACC ACAGGTGCGG 
CGCCCGATCC ACCCATCCCT TAGCCAGCAG CGCAGCCTAC AACCGCTGTC TTTCCAAAAC 
CCTGTTTACC ACCTCAGCAA CCTGCACGCA CAATCAACAC ACTCAACCCA GTCCACACAC 
TCTGCCCAGT CCCTGCAGCC TGACTCTAGC TCAGAAAACC TGAGCACAGG GAGCTCTCGG 
AGCGCCAGTC CTAGTGCCTC AGGTGGCCGG GGAGCAACTC CCAGAGCCCG GATACCCTCC 
ACAAGCAGCG TGGAGGAGGA ATTTAGCCGA AGAAGCATCC AGGGACAGGA GACGCCGCCT 
CCAACGGCAC GCTGGCACCC TCCCCTGGCA GACCAGCACT CGGGAGCCCA GGTGGTGGCA 
GTGCCAAGGC AGAGTACCAC CGGTACGGCA CATATAGTGA AAGTGGAGCA GCAGAGTCGA 
GGAGTTGGGT TGGTGGCAGC AAATGCTGGA GCACGAACCC CAAGGTCGCT CCCTCACAGC 
ACGTCCATCC GCAGCGGCAG CAGCGCCAAC ACTGAACCCA TACAGCAGAG CGGTGAACGA 
TCAAGACAGC AGTCCACGTG CTCCAAAGAT GGTTCAGTAC CAGGAGGACG AGCCAACAAA 
CAGGTCCAGT CACCTGTAGA GTCTGTCACC ATGTCCCCAG TGGAGCGAAC AGCTGCGTGG 
GTTTTGAATA ATGGCCAGTA TGAAGATGAG GAAGAGGAAG GGCAGAGTAA AGACGATGCC 
AAACATGTGG AGAAGTATGA ACAGGAAATC TCGAAGCTAA AGGAGCGTCT GCGGACGTCT 
AGTCGGCGGC TGGAGGAGTA TGAGAGACGG TTACTGGCTC AGGAGCAGCA GATGCAGAAG 
CTGCTCTTAG AATACAAGAC TCGACTGGAG GACAGTGAGG ACCGTCTGCG AAGACAACAG 
GAGGAAAAAG ACAGCCAGAT GAAGAGTATT ATCTGCAGGC TGATGGCCGT GGAGGAAGAG 
CTGAAGAGAG ACCATGCAGA GATGCAGGCA GTCATAGATG CCAAACAGAA GATAATCGAT 
GCACAGGAGA AGAGGATCAG CTCTCTGGAT GCTGCTAACT CTCGGCTGAT GAGCGCTCTG 
ACTCAGGTGA AGGAGCGCTA CAGCATGCAC AACCTCCGGA ACGGCCTGTC ACCCACCAAC 
CCCACCAAAC TCTCCATCAC AGAGAACGGG GAGTTCAAGA ATAGCAGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171381.1
CDS	456..3989
Translation	MGNSCDRAEASAERSPRRRSISGLGSSEKNISIDGPNSSPFKVPGFLSKRLKGSIKRTKSQTKLDRNTSFRLPSLRPTENDRSRGLPKLKESCSHESLLSPGSAVEALDLSMEEDVYIKPLHSSILGQDFCFEVAYSGGSKCFSCSSAAERDKWMENLKRTVQPNKDNCRRAENVLRLWIIEAKDLPPKKKYFCELCLDDMLYARTTSKTRSDCLFWGEHFEFSGLPSMKSITIHIYRDVDKKKKKDKNNYVGLVNIPVHGVMGRQFVEKWYPVSTPTTSKAKGGGPSIRIKSRFQTISILPMEQYKEFAEFVTNNYTMLCSVLEPVISVKNKEEMASALVHILQSTGRAKDFLTDLVMSEVDRCADHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDQSKCTSNELPEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCHARGKQDISQRLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWAGMTRFLLEISNLETISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKGDNSFLQATVAKLGPLPRILGDITRSLSSPTPIQQQLRRFQDHSSAHDISGSVSSGLQRIFEDPADSEMRSIKSPVQEHMEALVRGKHPLLGQQSSAHSMSFSDKEERDSPLPNGRSISLMDLQDSYLAQGHPGPNSLNEASSRLGRVGSQASIGPVPPPHLHQPPVHPKVPQLRDNLPQSAPQVRRPIHPSLSQQRSLQPLSFQNPVYHLSNLHAQSTHSTQSTHSAQSLQPDSSSENLSTGSSRSASPSASGGRGATPRARIPSTSSVEEEFSRRSIQGQETPPPTARWHPPLADQHSGAQVVAVPRQSTTGTAHIVKVEQQSRGVGLVAANAGARTPRSLPHSTSIRSGSSANTEPIQQSGERSRQQSTCSKDGSVPGGRANKQVQSPVESVTMSPVERTAAWVLNNGQYEDEEEEGQSKDDAKHVEKYEQEISKLKERLRTSSRRLEEYERRLLAQEQQMQKLLLEYKTRLEDSEDRLRRQQEEKDSQMKSIICRLMAVEEELKRDHAEMQAVIDAKQKIIDAQEKRISSLDAANSRLMSALTQVKERYSMHNLRNGLSPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_005171324.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171324.4

ORF Insert Sequence:

ATGGGCAACA GCTGTGATAG AGCGGAAGCG TCCGCGGAGC GCTCTCCCAG ACGGAGGAGC 
ATATCAGGGC TAGGCAGCTC AGAGAAGAAC ATTTCCATCG ATGGCCCCAA CTCATCTCCC 
TTCAAAGTGC CTGGTTTTTT AAGTAAACGA CTGAAAGGAT CCATCAAAAG GACGAAGAGC 
CAGACTAAGC TGGACCGCAA CACAAGTTTC AGACTCCCAT CGCTGCGACC AACAGAAAAT 
GACAGGTCAC GAGGCCTGCC CAAATTGAAG GAGTCCTGTT CCCATGAGTC TTTGCTGAGT 
CCTGGCAGCG CTGTTGAAGC TCTGGATCTG AGTATGGAGG AGGATGTCTA CATCAAACCT 
TTACACAGCA GCATCCTAGG ACAAGATTTC TGCTTTGAGG TGGCGTACTC AGGTGGAAGT 
AAGTGCTTCA GCTGTTCCTC TGCTGCTGAA CGGGACAAAT GGATGGAGAA CCTCAAAAGA 
ACTGTGCAAC CTAATAAGGA TAATTGCCGG CGGGCGGAAA ACGTCCTCCG CCTGTGGATC 
ATTGAGGCAA AAGACCTGCC ACCAAAAAAG AAATATTTCT GTGAGCTGTG TCTGGATGAC 
ATGCTGTACG CTCGTACCAC CAGCAAAACC CGCTCCGACT GCCTGTTCTG GGGGGAACAC 
TTTGAGTTTT CAGGCCTCCC GTCCATGAAA AGCATCACCA TACACATCTA CCGGGATGTG 
GACAAGAAAA AGAAAAAGGA CAAAAACAAC TATGTTGGCC TGGTGAACAT CCCTGTGCAT 
GGGGTGATGG GTAGGCAGTT TGTGGAGAAG TGGTATCCAG TCAGCACTCC CACCACCAGC 
AAAGCCAAAG GTGGAGGCCC CTCCATCCGC ATCAAATCCC GCTTTCAGAC CATCTCAATT 
CTGCCCATGG AGCAGTACAA GGAGTTTGCG GAGTTTGTTA CCAATAACTA TACCATGTTG 
TGCTCTGTGT TGGAGCCGGT CATAAGCGTG AAGAACAAAG AGGAGATGGC CAGCGCTCTG 
GTGCACATTC TGCAGAGCAC GGGACGGGCA AAGGACTTCC TAACGGATTT GGTGATGTCA 
GAAGTGGATC GCTGTGCTGA CCATGATGTG CTGATCTTTA GAGAAAACAC ACTAGCCACC 
AAAGCCATTG AAGAATACCT CAAACTGGTG GGACAGAAGT ACCTACATGA TGCACTAGGG 
GAGTTTATTA AAGCCCTGTA CGAGTCGGAT GAAAATTGTG AAGTAGACCA ATCAAAGTGC 
ACCAGCAACG AGCTTCCAGA GCATCAGAGT AACCTGAAGA TGTGCTGCGA ACTGGCCTTC 
TGCAAGATCA TCAACTCTTA TTGTGTTTTT CCACGTGAAC TGAAGGAGGT CTTTGCATCA 
TGGAAGCAGC AGTGTCACGC TCGAGGGAAG CAGGACATCA GTCAGCGTCT TATCAGCGCA 
TCGCTCTTCC TGCGCTTTCT GTGTCCTGCC ATCATGTCCC CGTCACTCTT TGGTCTCATG 
CAGGAATATC CAGATGACCG TACTTCTCGA ACACTCACCC TCATTGCCAA AGTCATTCAG 
AATCTTGCTA ACTTTGCCAA ATTCGGGAAC AAAGAGGAGT ACATGGCTTT CATGAATGAC 
TTTCTAGAGC ACGAGTGGGC TGGTATGACC CGTTTTCTGC TTGAGATCTC GAATCTTGAG 
ACAATCTCCA ACACCCCGGG CTTTGAGGGC TACATTGACC TGGGCCGGGA GCTGTCAGTG 
CTTCACTCGC TGCTCTGGGA GGTGGTGTCT CAGCTGGACA AGGGTGACAA TTCCTTCCTG 
CAGGCGACGG TGGCCAAACT GGGGCCCCTG CCACGTATCC TTGGCGATAT CACCCGCTCG 
CTGTCCAGCC CTACTCCCAT CCAGCAGCAG CTCAGGCGCT TCCAGGACCA CAGCTCTGCT 
CATGACATCA GTGGAAGTGT GTCCTCAGGA CTGCAGAGAA TATTTGAAGA CCCAGCAGAC 
AGTGAAATGA GGAGCATCAA ATCACCTGTC CAAGAGCACA TGGAGGCTTT AGTCAGAGGA 
AAGCATCCTT TACTGGGTCA ACAGTCGTCC GCACACAGCA TGAGCTTCTC CGACAAAGAG 
GAAAGGGACA GTCCGCTCCC GAATGGACGC AGTATATCTT TAATGGACTT GCAGGACTCT 
TACCTTGCTC AGGGGCATCC AGGTCCCAAC TCGCTCAATG AAGCCTCATC TAGGTTAGGC 
AGAGTAGGCT CTCAGGCCTC CATCGGCCCT GTCCCTCCTC CACACCTCCA CCAGCCTCCA 
GTCCACCCGA AAGTTCCACA GCTGAGGGAT AACTTACCCC AGAGTGCACC ACAGGTGCGG 
CGCCCGATCC ACCCATCCCT TAGCCAGCAG CGCAGCCTAC AACCGCTGTC TTTCCAAAAC 
CCTGTTTACC ACCTCAGCAA CCTGCACGCA CAATCAACAC ACTCAACCCA GTCCACACAC 
TCTGCCCAGT CCCTGCAGCC TGACTCTAGC TCAGAAAACC TGAGCACAGG GAGCTCTCGG 
AGCGCCAGTC CTAGTGCCTC AGGTGGCCGG GGAGCAACTC CCAGAGCCCG GATACCCTCC 
ACAAGCAGCG TGGAGGAGGA ATTTAGCCGA AGAAGCATCC AGGGACAGGA GACGCCGCCT 
CCAACGGCAC GCTGGCACCC TCCCCTGGCA GACCAGCACT CGGGAGCCCA GGTGGTGGCA 
GTGCCAAGGC AGAGTACCAC CGGTACGGCA CATATAGTGA AAGTGGAGCA GCAGAGTCGA 
GGAGTTGGGT TGGTGGCAGC AAATGCTGGA GCACGAACCC CAAGGTCGCT CCCTCACAGC 
ACGTCCATCC GCAGCGGCAG CAGCGCCAAC ACTGAACCCA TACAGCAGAG CGGTGAACGA 
TCAAGACAGC AGTCCACGTG CTCCAAAGAT GGTTCAGTAC CAGGAGGACG AGCCAACAAA 
CAGGTCCAGT CACCTGTAGA GTCTGTCACC ATGTCCCCAG TGGAGCGAAC AGCTGCGTGG 
GTTTTGAATA ATGGCCAGTA TGAAGATGAG GAAGAGGAAG GGCAGAGTAA AGACGATGCC 
AAACATGTGG AGAAGTATGA ACAGGAAATC TCGAAGCTAA AGGAGCGTCT GCGGACGTCT 
AGTCGGCGGC TGGAGGAGTA TGAGAGACGG TTACTGGCTC AGGAGCAGCA GATGCAGAAG 
CTGCTCTTAG AATACAAGAC TCGACTGGAG GACAGTGAGG ACCGTCTGCG AAGACAACAG 
GAGGAAAAAG ACAGCCAGAT GAAGAGTATT ATCTGCAGGC TGATGGCCGT GGAGGAAGAG 
CTGAAGAGAG ACCATGCAGA GATGCAGGCA GTCATAGATG CCAAACAGAA GATAATCGAT 
GCACAGGAGA AGAGGATCAG CTCTCTGGAT GCTGCTAACT CTCGGCTGAT GAGCGCTCTG 
ACTCAGGTGA AGGAGCGCTA CAGCATGCAC AACCTCCGGA ACGGCCTGTC ACCCACCAAC 
CCCACCAAAC TCTCCATCAC AGAGAACGGG GAGTTCAAGA ATAGCAGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasal2 ( XM_005171324.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171381.1 Danio rerio RAS protein activator like 2 (rasal2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa07240
Clone ID Related Accession (Same CDS sequence)	XM_005171324.4 , XM_005171324.3
Accession Version	XM_005171324.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras GTPase-activating protein nGAP isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171324.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAACA GCTGTGATAG AGCGGAAGCG TCCGCGGAGC GCTCTCCCAG ACGGAGGAGC 
ATATCAGGGC TAGGCAGCTC AGAGAAGAAC ATTTCCATCG ATGGCCCCAA CTCATCTCCC 
TTCAAAGTGC CTGGTTTTTT AAGTAAACGA CTGAAAGGAT CCATCAAAAG GACGAAGAGC 
CAGACTAAGC TGGACCGCAA CACAAGTTTC AGACTCCCAT CGCTGCGACC AACAGAAAAT 
GACAGGTCAC GAGGCCTGCC CAAATTGAAG GAGTCCTGTT CCCATGAGTC TTTGCTGAGT 
CCTGGCAGCG CTGTTGAAGC TCTGGATCTG AGTATGGAGG AGGATGTCTA CATCAAACCT 
TTACACAGCA GCATCCTAGG ACAAGATTTC TGCTTTGAGG TGGCGTACTC AGGTGGAAGT 
AAGTGCTTCA GCTGTTCCTC TGCTGCTGAA CGGGACAAAT GGATGGAGAA CCTCAAAAGA 
ACTGTGCAAC CTAATAAGGA TAATTGCCGG CGGGCGGAAA ACGTCCTCCG CCTGTGGATC 
ATTGAGGCAA AAGACCTGCC ACCAAAAAAG AAATATTTCT GTGAGCTGTG TCTGGATGAC 
ATGCTGTACG CTCGTACCAC CAGCAAAACC CGCTCCGACT GCCTGTTCTG GGGGGAACAC 
TTTGAGTTTT CAGGCCTCCC GTCCATGAAA AGCATCACCA TACACATCTA CCGGGATGTG 
GACAAGAAAA AGAAAAAGGA CAAAAACAAC TATGTTGGCC TGGTGAACAT CCCTGTGCAT 
GGGGTGATGG GTAGGCAGTT TGTGGAGAAG TGGTATCCAG TCAGCACTCC CACCACCAGC 
AAAGCCAAAG GTGGAGGCCC CTCCATCCGC ATCAAATCCC GCTTTCAGAC CATCTCAATT 
CTGCCCATGG AGCAGTACAA GGAGTTTGCG GAGTTTGTTA CCAATAACTA TACCATGTTG 
TGCTCTGTGT TGGAGCCGGT CATAAGCGTG AAGAACAAAG AGGAGATGGC CAGCGCTCTG 
GTGCACATTC TGCAGAGCAC GGGACGGGCA AAGGACTTCC TAACGGATTT GGTGATGTCA 
GAAGTGGATC GCTGTGCTGA CCATGATGTG CTGATCTTTA GAGAAAACAC ACTAGCCACC 
AAAGCCATTG AAGAATACCT CAAACTGGTG GGACAGAAGT ACCTACATGA TGCACTAGGG 
GAGTTTATTA AAGCCCTGTA CGAGTCGGAT GAAAATTGTG AAGTAGACCA ATCAAAGTGC 
ACCAGCAACG AGCTTCCAGA GCATCAGAGT AACCTGAAGA TGTGCTGCGA ACTGGCCTTC 
TGCAAGATCA TCAACTCTTA TTGTGTTTTT CCACGTGAAC TGAAGGAGGT CTTTGCATCA 
TGGAAGCAGC AGTGTCACGC TCGAGGGAAG CAGGACATCA GTCAGCGTCT TATCAGCGCA 
TCGCTCTTCC TGCGCTTTCT GTGTCCTGCC ATCATGTCCC CGTCACTCTT TGGTCTCATG 
CAGGAATATC CAGATGACCG TACTTCTCGA ACACTCACCC TCATTGCCAA AGTCATTCAG 
AATCTTGCTA ACTTTGCCAA ATTCGGGAAC AAAGAGGAGT ACATGGCTTT CATGAATGAC 
TTTCTAGAGC ACGAGTGGGC TGGTATGACC CGTTTTCTGC TTGAGATCTC GAATCTTGAG 
ACAATCTCCA ACACCCCGGG CTTTGAGGGC TACATTGACC TGGGCCGGGA GCTGTCAGTG 
CTTCACTCGC TGCTCTGGGA GGTGGTGTCT CAGCTGGACA AGGGTGACAA TTCCTTCCTG 
CAGGCGACGG TGGCCAAACT GGGGCCCCTG CCACGTATCC TTGGCGATAT CACCCGCTCG 
CTGTCCAGCC CTACTCCCAT CCAGCAGCAG CTCAGGCGCT TCCAGGACCA CAGCTCTGCT 
CATGACATCA GTGGAAGTGT GTCCTCAGGA CTGCAGAGAA TATTTGAAGA CCCAGCAGAC 
AGTGAAATGA GGAGCATCAA ATCACCTGTC CAAGAGCACA TGGAGGCTTT AGTCAGAGGA 
AAGCATCCTT TACTGGGTCA ACAGTCGTCC GCACACAGCA TGAGCTTCTC CGACAAAGAG 
GAAAGGGACA GTCCGCTCCC GAATGGACGC AGTATATCTT TAATGGACTT GCAGGACTCT 
TACCTTGCTC AGGGGCATCC AGGTCCCAAC TCGCTCAATG AAGCCTCATC TAGGTTAGGC 
AGAGTAGGCT CTCAGGCCTC CATCGGCCCT GTCCCTCCTC CACACCTCCA CCAGCCTCCA 
GTCCACCCGA AAGTTCCACA GCTGAGGGAT AACTTACCCC AGAGTGCACC ACAGGTGCGG 
CGCCCGATCC ACCCATCCCT TAGCCAGCAG CGCAGCCTAC AACCGCTGTC TTTCCAAAAC 
CCTGTTTACC ACCTCAGCAA CCTGCACGCA CAATCAACAC ACTCAACCCA GTCCACACAC 
TCTGCCCAGT CCCTGCAGCC TGACTCTAGC TCAGAAAACC TGAGCACAGG GAGCTCTCGG 
AGCGCCAGTC CTAGTGCCTC AGGTGGCCGG GGAGCAACTC CCAGAGCCCG GATACCCTCC 
ACAAGCAGCG TGGAGGAGGA ATTTAGCCGA AGAAGCATCC AGGGACAGGA GACGCCGCCT 
CCAACGGCAC GCTGGCACCC TCCCCTGGCA GACCAGCACT CGGGAGCCCA GGTGGTGGCA 
GTGCCAAGGC AGAGTACCAC CGGTACGGCA CATATAGTGA AAGTGGAGCA GCAGAGTCGA 
GGAGTTGGGT TGGTGGCAGC AAATGCTGGA GCACGAACCC CAAGGTCGCT CCCTCACAGC 
ACGTCCATCC GCAGCGGCAG CAGCGCCAAC ACTGAACCCA TACAGCAGAG CGGTGAACGA 
TCAAGACAGC AGTCCACGTG CTCCAAAGAT GGTTCAGTAC CAGGAGGACG AGCCAACAAA 
CAGGTCCAGT CACCTGTAGA GTCTGTCACC ATGTCCCCAG TGGAGCGAAC AGCTGCGTGG 
GTTTTGAATA ATGGCCAGTA TGAAGATGAG GAAGAGGAAG GGCAGAGTAA AGACGATGCC 
AAACATGTGG AGAAGTATGA ACAGGAAATC TCGAAGCTAA AGGAGCGTCT GCGGACGTCT 
AGTCGGCGGC TGGAGGAGTA TGAGAGACGG TTACTGGCTC AGGAGCAGCA GATGCAGAAG 
CTGCTCTTAG AATACAAGAC TCGACTGGAG GACAGTGAGG ACCGTCTGCG AAGACAACAG 
GAGGAAAAAG ACAGCCAGAT GAAGAGTATT ATCTGCAGGC TGATGGCCGT GGAGGAAGAG 
CTGAAGAGAG ACCATGCAGA GATGCAGGCA GTCATAGATG CCAAACAGAA GATAATCGAT 
GCACAGGAGA AGAGGATCAG CTCTCTGGAT GCTGCTAACT CTCGGCTGAT GAGCGCTCTG 
ACTCAGGTGA AGGAGCGCTA CAGCATGCAC AACCTCCGGA ACGGCCTGTC ACCCACCAAC 
CCCACCAAAC TCTCCATCAC AGAGAACGGG GAGTTCAAGA ATAGCAGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171381.1
CDS	16..3549
Translation	MGNSCDRAEASAERSPRRRSISGLGSSEKNISIDGPNSSPFKVPGFLSKRLKGSIKRTKSQTKLDRNTSFRLPSLRPTENDRSRGLPKLKESCSHESLLSPGSAVEALDLSMEEDVYIKPLHSSILGQDFCFEVAYSGGSKCFSCSSAAERDKWMENLKRTVQPNKDNCRRAENVLRLWIIEAKDLPPKKKYFCELCLDDMLYARTTSKTRSDCLFWGEHFEFSGLPSMKSITIHIYRDVDKKKKKDKNNYVGLVNIPVHGVMGRQFVEKWYPVSTPTTSKAKGGGPSIRIKSRFQTISILPMEQYKEFAEFVTNNYTMLCSVLEPVISVKNKEEMASALVHILQSTGRAKDFLTDLVMSEVDRCADHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDQSKCTSNELPEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCHARGKQDISQRLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWAGMTRFLLEISNLETISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKGDNSFLQATVAKLGPLPRILGDITRSLSSPTPIQQQLRRFQDHSSAHDISGSVSSGLQRIFEDPADSEMRSIKSPVQEHMEALVRGKHPLLGQQSSAHSMSFSDKEERDSPLPNGRSISLMDLQDSYLAQGHPGPNSLNEASSRLGRVGSQASIGPVPPPHLHQPPVHPKVPQLRDNLPQSAPQVRRPIHPSLSQQRSLQPLSFQNPVYHLSNLHAQSTHSTQSTHSAQSLQPDSSSENLSTGSSRSASPSASGGRGATPRARIPSTSSVEEEFSRRSIQGQETPPPTARWHPPLADQHSGAQVVAVPRQSTTGTAHIVKVEQQSRGVGLVAANAGARTPRSLPHSTSIRSGSSANTEPIQQSGERSRQQSTCSKDGSVPGGRANKQVQSPVESVTMSPVERTAAWVLNNGQYEDEEEEGQSKDDAKHVEKYEQEISKLKERLRTSSRRLEEYERRLLAQEQQMQKLLLEYKTRLEDSEDRLRRQQEEKDSQMKSIICRLMAVEEELKRDHAEMQAVIDAKQKIIDAQEKRISSLDAANSRLMSALTQVKERYSMHNLRNGLSPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_005171324.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171324.3

ORF Insert Sequence:

ATGGGCAACA GCTGTGATAG AGCGGAAGCG TCCGCGGAGC GCTCTCCCAG ACGGAGGAGC 
ATATCAGGGC TAGGCAGCTC AGAGAAGAAC ATTTCCATCG ATGGCCCCAA CTCATCTCCC 
TTCAAAGTGC CTGGTTTTTT AAGTAAACGA CTGAAAGGAT CCATCAAAAG GACGAAGAGC 
CAGACTAAGC TGGACCGCAA CACAAGTTTC AGACTCCCAT CGCTGCGACC AACAGAAAAT 
GACAGGTCAC GAGGCCTGCC CAAATTGAAG GAGTCCTGTT CCCATGAGTC TTTGCTGAGT 
CCTGGCAGCG CTGTTGAAGC TCTGGATCTG AGTATGGAGG AGGATGTCTA CATCAAACCT 
TTACACAGCA GCATCCTAGG ACAAGATTTC TGCTTTGAGG TGGCGTACTC AGGTGGAAGT 
AAGTGCTTCA GCTGTTCCTC TGCTGCTGAA CGGGACAAAT GGATGGAGAA CCTCAAAAGA 
ACTGTGCAAC CTAATAAGGA TAATTGCCGG CGGGCGGAAA ACGTCCTCCG CCTGTGGATC 
ATTGAGGCAA AAGACCTGCC ACCAAAAAAG AAATATTTCT GTGAGCTGTG TCTGGATGAC 
ATGCTGTACG CTCGTACCAC CAGCAAAACC CGCTCCGACT GCCTGTTCTG GGGGGAACAC 
TTTGAGTTTT CAGGCCTCCC GTCCATGAAA AGCATCACCA TACACATCTA CCGGGATGTG 
GACAAGAAAA AGAAAAAGGA CAAAAACAAC TATGTTGGCC TGGTGAACAT CCCTGTGCAT 
GGGGTGATGG GTAGGCAGTT TGTGGAGAAG TGGTATCCAG TCAGCACTCC CACCACCAGC 
AAAGCCAAAG GTGGAGGCCC CTCCATCCGC ATCAAATCCC GCTTTCAGAC CATCTCAATT 
CTGCCCATGG AGCAGTACAA GGAGTTTGCG GAGTTTGTTA CCAATAACTA TACCATGTTG 
TGCTCTGTGT TGGAGCCGGT CATAAGCGTG AAGAACAAAG AGGAGATGGC CAGCGCTCTG 
GTGCACATTC TGCAGAGCAC GGGACGGGCA AAGGACTTCC TAACGGATTT GGTGATGTCA 
GAAGTGGATC GCTGTGCTGA CCATGATGTG CTGATCTTTA GAGAAAACAC ACTAGCCACC 
AAAGCCATTG AAGAATACCT CAAACTGGTG GGACAGAAGT ACCTACATGA TGCACTAGGG 
GAGTTTATTA AAGCCCTGTA CGAGTCGGAT GAAAATTGTG AAGTAGACCA ATCAAAGTGC 
ACCAGCAACG AGCTTCCAGA GCATCAGAGT AACCTGAAGA TGTGCTGCGA ACTGGCCTTC 
TGCAAGATCA TCAACTCTTA TTGTGTTTTT CCACGTGAAC TGAAGGAGGT CTTTGCATCA 
TGGAAGCAGC AGTGTCACGC TCGAGGGAAG CAGGACATCA GTCAGCGTCT TATCAGCGCA 
TCGCTCTTCC TGCGCTTTCT GTGTCCTGCC ATCATGTCCC CGTCACTCTT TGGTCTCATG 
CAGGAATATC CAGATGACCG TACTTCTCGA ACACTCACCC TCATTGCCAA AGTCATTCAG 
AATCTTGCTA ACTTTGCCAA ATTCGGGAAC AAAGAGGAGT ACATGGCTTT CATGAATGAC 
TTTCTAGAGC ACGAGTGGGC TGGTATGACC CGTTTTCTGC TTGAGATCTC GAATCTTGAG 
ACAATCTCCA ACACCCCGGG CTTTGAGGGC TACATTGACC TGGGCCGGGA GCTGTCAGTG 
CTTCACTCGC TGCTCTGGGA GGTGGTGTCT CAGCTGGACA AGGGTGACAA TTCCTTCCTG 
CAGGCGACGG TGGCCAAACT GGGGCCCCTG CCACGTATCC TTGGCGATAT CACCCGCTCG 
CTGTCCAGCC CTACTCCCAT CCAGCAGCAG CTCAGGCGCT TCCAGGACCA CAGCTCTGCT 
CATGACATCA GTGGAAGTGT GTCCTCAGGA CTGCAGAGAA TATTTGAAGA CCCAGCAGAC 
AGTGAAATGA GGAGCATCAA ATCACCTGTC CAAGAGCACA TGGAGGCTTT AGTCAGAGGA 
AAGCATCCTT TACTGGGTCA ACAGTCGTCC GCACACAGCA TGAGCTTCTC CGACAAAGAG 
GAAAGGGACA GTCCGCTCCC GAATGGACGC AGTATATCTT TAATGGACTT GCAGGACTCT 
TACCTTGCTC AGGGGCATCC AGGTCCCAAC TCGCTCAATG AAGCCTCATC TAGGTTAGGC 
AGAGTAGGCT CTCAGGCCTC CATCGGCCCT GTCCCTCCTC CACACCTCCA CCAGCCTCCA 
GTCCACCCGA AAGTTCCACA GCTGAGGGAT AACTTACCCC AGAGTGCACC ACAGGTGCGG 
CGCCCGATCC ACCCATCCCT TAGCCAGCAG CGCAGCCTAC AACCGCTGTC TTTCCAAAAC 
CCTGTTTACC ACCTCAGCAA CCTGCACGCA CAATCAACAC ACTCAACCCA GTCCACACAC 
TCTGCCCAGT CCCTGCAGCC TGACTCTAGC TCAGAAAACC TGAGCACAGG GAGCTCTCGG 
AGCGCCAGTC CTAGTGCCTC AGGTGGCCGG GGAGCAACTC CCAGAGCCCG GATACCCTCC 
ACAAGCAGCG TGGAGGAGGA ATTTAGCCGA AGAAGCATCC AGGGACAGGA GACGCCGCCT 
CCAACGGCAC GCTGGCACCC TCCCCTGGCA GACCAGCACT CGGGAGCCCA GGTGGTGGCA 
GTGCCAAGGC AGAGTACCAC CGGTACGGCA CATATAGTGA AAGTGGAGCA GCAGAGTCGA 
GGAGTTGGGT TGGTGGCAGC AAATGCTGGA GCACGAACCC CAAGGTCGCT CCCTCACAGC 
ACGTCCATCC GCAGCGGCAG CAGCGCCAAC ACTGAACCCA TACAGCAGAG CGGTGAACGA 
TCAAGACAGC AGTCCACGTG CTCCAAAGAT GGTTCAGTAC CAGGAGGACG AGCCAACAAA 
CAGGTCCAGT CACCTGTAGA GTCTGTCACC ATGTCCCCAG TGGAGCGAAC AGCTGCGTGG 
GTTTTGAATA ATGGCCAGTA TGAAGATGAG GAAGAGGAAG GGCAGAGTAA AGACGATGCC 
AAACATGTGG AGAAGTATGA ACAGGAAATC TCGAAGCTAA AGGAGCGTCT GCGGACGTCT 
AGTCGGCGGC TGGAGGAGTA TGAGAGACGG TTACTGGCTC AGGAGCAGCA GATGCAGAAG 
CTGCTCTTAG AATACAAGAC TCGACTGGAG GACAGTGAGG ACCGTCTGCG AAGACAACAG 
GAGGAAAAAG ACAGCCAGAT GAAGAGTATT ATCTGCAGGC TGATGGCCGT GGAGGAAGAG 
CTGAAGAGAG ACCATGCAGA GATGCAGGCA GTCATAGATG CCAAACAGAA GATAATCGAT 
GCACAGGAGA AGAGGATCAG CTCTCTGGAT GCTGCTAACT CTCGGCTGAT GAGCGCTCTG 
ACTCAGGTGA AGGAGCGCTA CAGCATGCAC AACCTCCGGA ACGGCCTGTC ACCCACCAAC 
CCCACCAAAC TCTCCATCAC AGAGAACGGG GAGTTCAAGA ATAGCAGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasal2 ( XM_005171325.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171382.1 Danio rerio RAS protein activator like 2 (rasal2), transcript variant X5, mRNA.

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CloneID	ODa07241
Clone ID Related Accession (Same CDS sequence)	XM_005171325.4 , XM_005171325.3
Accession Version	XM_005171325.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras GTPase-activating protein nGAP isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171325.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTCA TAGCGGAAGC GTCCGCGGAG CGCTCTCCCA GACGGAGGAG CATATCAGGG 
CTAGGCAGCT CAGAGAAGAA CATTTCCATC GATGGCCCCA ACTCATCTCC CTTCAAAGTG 
CCTGGTTTTT TAAGTAAACG ACTGAAAGGA TCCATCAAAA GGACGAAGAG CCAGACTAAG 
CTGGACCGCA ACACAAGTTT CAGACTCCCA TCGCTGCGAC CAACAGAAAA TGACAGGTCA 
CGAGGCCTGC CCAAATTGAA GGAGTCCTGT TCCCATGAGT CTTTGCTGAG TCCTGGCAGC 
GCTGTTGAAG CTCTGGATCT GAGTATGGAG GAGGATGTCT ACATCAAACC TTTACACAGC 
AGCATCCTAG GACAAGATTT CTGCTTTGAG GTGGCGTACT CAGGTGGAAG TAAGTGCTTC 
AGCTGTTCCT CTGCTGCTGA ACGGGACAAA TGGATGGAGA ACCTCAAAAG AACTGTGCAA 
CCTAATAAGG ATAATTGCCG GCGGGCGGAA AACGTCCTCC GCCTGTGGAT CATTGAGGCA 
AAAGACCTGC CACCAAAAAA GAAATATTTC TGTGAGCTGT GTCTGGATGA CATGCTGTAC 
GCTCGTACCA CCAGCAAAAC CCGCTCCGAC TGCCTGTTCT GGGGGGAACA CTTTGAGTTT 
TCAGGCCTCC CGTCCATGAA AAGCATCACC ATACACATCT ACCGGGATGT GGACAAGAAA 
AAGAAAAAGG ACAAAAACAA CTATGTTGGC CTGGTGAACA TCCCTGTGCA TGGGGTGATG 
GGTAGGCAGT TTGTGGAGAA GTGGTATCCA GTCAGCACTC CCACCACCAG CAAAGCCAAA 
GGTGGAGGCC CCTCCATCCG CATCAAATCC CGCTTTCAGA CCATCTCAAT TCTGCCCATG 
GAGCAGTACA AGGAGTTTGC GGAGTTTGTT ACCAATAACT ATACCATGTT GTGCTCTGTG 
TTGGAGCCGG TCATAAGCGT GAAGAACAAA GAGGAGATGG CCAGCGCTCT GGTGCACATT 
CTGCAGAGCA CGGGACGGGC AAAGGACTTC CTAACGGATT TGGTGATGTC AGAAGTGGAT 
CGCTGTGCTG ACCATGATGT GCTGATCTTT AGAGAAAACA CACTAGCCAC CAAAGCCATT 
GAAGAATACC TCAAACTGGT GGGACAGAAG TACCTACATG ATGCACTAGG GGAGTTTATT 
AAAGCCCTGT ACGAGTCGGA TGAAAATTGT GAAGTAGACC AATCAAAGTG CACCAGCAAC 
GAGCTTCCAG AGCATCAGAG TAACCTGAAG ATGTGCTGCG AACTGGCCTT CTGCAAGATC 
ATCAACTCTT ATTGTGTTTT TCCACGTGAA CTGAAGGAGG TCTTTGCATC ATGGAAGCAG 
CAGTGTCACG CTCGAGGGAA GCAGGACATC AGTCAGCGTC TTATCAGCGC ATCGCTCTTC 
CTGCGCTTTC TGTGTCCTGC CATCATGTCC CCGTCACTCT TTGGTCTCAT GCAGGAATAT 
CCAGATGACC GTACTTCTCG AACACTCACC CTCATTGCCA AAGTCATTCA GAATCTTGCT 
AACTTTGCCA AATTCGGGAA CAAAGAGGAG TACATGGCTT TCATGAATGA CTTTCTAGAG 
CACGAGTGGG CTGGTATGAC CCGTTTTCTG CTTGAGATCT CGAATCTTGA GACAATCTCC 
AACACCCCGG GCTTTGAGGG CTACATTGAC CTGGGCCGGG AGCTGTCAGT GCTTCACTCG 
CTGCTCTGGG AGGTGGTGTC TCAGCTGGAC AAGGGTGACA ATTCCTTCCT GCAGGCGACG 
GTGGCCAAAC TGGGGCCCCT GCCACGTATC CTTGGCGATA TCACCCGCTC GCTGTCCAGC 
CCTACTCCCA TCCAGCAGCA GCTCAGGCGC TTCCAGGACC ACAGCTCTGC TCATGACATC 
AGTGGAAGTG TGTCCTCAGG ACTGCAGAGA ATATTTGAAG ACCCAGCAGA CAGTGAAATG 
AGGAGCATCA AATCACCTGT CCAAGAGCAC ATGGAGGCTT TAGTCAGAGG AAAGCATCCT 
TTACTGGGTC AACAGTCGTC CGCACACAGC ATGAGCTTCT CCGACAAAGA GGAAAGGGAC 
AGTCCGCTCC CGAATGGACG CAGTATATCT TTAATGGACT TGCAGGACTC TTACCTTGCT 
CAGGGGCATC CAGGTCCCAA CTCGCTCAAT GAAGCCTCAT CTAGGTTAGG CAGAGTAGGC 
TCTCAGGCCT CCATCGGCCC TGTCCCTCCT CCACACCTCC ACCAGCCTCC AGTCCACCCG 
AAAGTTCCAC AGCTGAGGGA TAACTTACCC CAGAGTGCAC CACAGGTGCG GCGCCCGATC 
CACCCATCCC TTAGCCAGCA GCGCAGCCTA CAACCGCTGT CTTTCCAAAA CCCTGTTTAC 
CACCTCAGCA ACCTGCACGC ACAATCAACA CACTCAACCC AGTCCACACA CTCTGCCCAG 
TCCCTGCAGC CTGACTCTAG CTCAGAAAAC CTGAGCACAG GGAGCTCTCG GAGCGCCAGT 
CCTAGTGCCT CAGGTGGCCG GGGAGCAACT CCCAGAGCCC GGATACCCTC CACAAGCAGC 
GTGGAGGAGG AATTTAGCCG AAGAAGCATC CAGGGACAGG AGACGCCGCC TCCAACGGCA 
CGCTGGCACC CTCCCCTGGC AGACCAGCAC TCGGGAGCCC AGGTGGTGGC AGTGCCAAGG 
CAGAGTACCA CCGGTACGGC ACATATAGTG AAAGTGGAGC AGCAGAGTCG AGGAGTTGGG 
TTGGTGGCAG CAAATGCTGG AGCACGAACC CCAAGGTCGC TCCCTCACAG CACGTCCATC 
CGCAGCGGCA GCAGCGCCAA CACTGAACCC ATACAGCAGA GCGGTGAACG ATCAAGACAG 
CAGTCCACGT GCTCCAAAGA TGGTTCAGTA CCAGGAGGAC GAGCCAACAA ACAGGTCCAG 
TCACCTGTAG AGTCTGTCAC CATGTCCCCA GTGGAGCGAA CAGCTGCGTG GGTTTTGAAT 
AATGGCCAGT ATGAAGATGA GGAAGAGGAA GGGCAGAGTA AAGACGATGC CAAACATGTG 
GAGAAGTATG AACAGGAAAT CTCGAAGCTA AAGGAGCGTC TGCGGACGTC TAGTCGGCGG 
CTGGAGGAGT ATGAGAGACG GTTACTGGCT CAGGAGCAGC AGATGCAGAA GCTGCTCTTA 
GAATACAAGA CTCGACTGGA GGACAGTGAG GACCGTCTGC GAAGACAACA GGAGGAAAAA 
GACAGCCAGA TGAAGAGTAT TATCTGCAGG CTGATGGCCG TGGAGGAAGA GCTGAAGAGA 
GACCATGCAG AGATGCAGGC AGTCATAGAT GCCAAACAGA AGATAATCGA TGCACAGGAG 
AAGAGGATCA GCTCTCTGGA TGCTGCTAAC TCTCGGCTGA TGAGCGCTCT GACTCAGGTG 
AAGGAGCGCT ACAGCATGCA CAACCTCCGG AACGGCCTGT CACCCACCAA CCCCACCAAA 
CTCTCCATCA CAGAGAACGG GGAGTTCAAG AATAGCAGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171382.1
CDS	148..3672
Translation	MDFIAEASAERSPRRRSISGLGSSEKNISIDGPNSSPFKVPGFLSKRLKGSIKRTKSQTKLDRNTSFRLPSLRPTENDRSRGLPKLKESCSHESLLSPGSAVEALDLSMEEDVYIKPLHSSILGQDFCFEVAYSGGSKCFSCSSAAERDKWMENLKRTVQPNKDNCRRAENVLRLWIIEAKDLPPKKKYFCELCLDDMLYARTTSKTRSDCLFWGEHFEFSGLPSMKSITIHIYRDVDKKKKKDKNNYVGLVNIPVHGVMGRQFVEKWYPVSTPTTSKAKGGGPSIRIKSRFQTISILPMEQYKEFAEFVTNNYTMLCSVLEPVISVKNKEEMASALVHILQSTGRAKDFLTDLVMSEVDRCADHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDQSKCTSNELPEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCHARGKQDISQRLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWAGMTRFLLEISNLETISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKGDNSFLQATVAKLGPLPRILGDITRSLSSPTPIQQQLRRFQDHSSAHDISGSVSSGLQRIFEDPADSEMRSIKSPVQEHMEALVRGKHPLLGQQSSAHSMSFSDKEERDSPLPNGRSISLMDLQDSYLAQGHPGPNSLNEASSRLGRVGSQASIGPVPPPHLHQPPVHPKVPQLRDNLPQSAPQVRRPIHPSLSQQRSLQPLSFQNPVYHLSNLHAQSTHSTQSTHSAQSLQPDSSSENLSTGSSRSASPSASGGRGATPRARIPSTSSVEEEFSRRSIQGQETPPPTARWHPPLADQHSGAQVVAVPRQSTTGTAHIVKVEQQSRGVGLVAANAGARTPRSLPHSTSIRSGSSANTEPIQQSGERSRQQSTCSKDGSVPGGRANKQVQSPVESVTMSPVERTAAWVLNNGQYEDEEEEGQSKDDAKHVEKYEQEISKLKERLRTSSRRLEEYERRLLAQEQQMQKLLLEYKTRLEDSEDRLRRQQEEKDSQMKSIICRLMAVEEELKRDHAEMQAVIDAKQKIIDAQEKRISSLDAANSRLMSALTQVKERYSMHNLRNGLSPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_005171325.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171325.4

ORF Insert Sequence:

ATGGATTTCA TAGCGGAAGC GTCCGCGGAG CGCTCTCCCA GACGGAGGAG CATATCAGGG 
CTAGGCAGCT CAGAGAAGAA CATTTCCATC GATGGCCCCA ACTCATCTCC CTTCAAAGTG 
CCTGGTTTTT TAAGTAAACG ACTGAAAGGA TCCATCAAAA GGACGAAGAG CCAGACTAAG 
CTGGACCGCA ACACAAGTTT CAGACTCCCA TCGCTGCGAC CAACAGAAAA TGACAGGTCA 
CGAGGCCTGC CCAAATTGAA GGAGTCCTGT TCCCATGAGT CTTTGCTGAG TCCTGGCAGC 
GCTGTTGAAG CTCTGGATCT GAGTATGGAG GAGGATGTCT ACATCAAACC TTTACACAGC 
AGCATCCTAG GACAAGATTT CTGCTTTGAG GTGGCGTACT CAGGTGGAAG TAAGTGCTTC 
AGCTGTTCCT CTGCTGCTGA ACGGGACAAA TGGATGGAGA ACCTCAAAAG AACTGTGCAA 
CCTAATAAGG ATAATTGCCG GCGGGCGGAA AACGTCCTCC GCCTGTGGAT CATTGAGGCA 
AAAGACCTGC CACCAAAAAA GAAATATTTC TGTGAGCTGT GTCTGGATGA CATGCTGTAC 
GCTCGTACCA CCAGCAAAAC CCGCTCCGAC TGCCTGTTCT GGGGGGAACA CTTTGAGTTT 
TCAGGCCTCC CGTCCATGAA AAGCATCACC ATACACATCT ACCGGGATGT GGACAAGAAA 
AAGAAAAAGG ACAAAAACAA CTATGTTGGC CTGGTGAACA TCCCTGTGCA TGGGGTGATG 
GGTAGGCAGT TTGTGGAGAA GTGGTATCCA GTCAGCACTC CCACCACCAG CAAAGCCAAA 
GGTGGAGGCC CCTCCATCCG CATCAAATCC CGCTTTCAGA CCATCTCAAT TCTGCCCATG 
GAGCAGTACA AGGAGTTTGC GGAGTTTGTT ACCAATAACT ATACCATGTT GTGCTCTGTG 
TTGGAGCCGG TCATAAGCGT GAAGAACAAA GAGGAGATGG CCAGCGCTCT GGTGCACATT 
CTGCAGAGCA CGGGACGGGC AAAGGACTTC CTAACGGATT TGGTGATGTC AGAAGTGGAT 
CGCTGTGCTG ACCATGATGT GCTGATCTTT AGAGAAAACA CACTAGCCAC CAAAGCCATT 
GAAGAATACC TCAAACTGGT GGGACAGAAG TACCTACATG ATGCACTAGG GGAGTTTATT 
AAAGCCCTGT ACGAGTCGGA TGAAAATTGT GAAGTAGACC AATCAAAGTG CACCAGCAAC 
GAGCTTCCAG AGCATCAGAG TAACCTGAAG ATGTGCTGCG AACTGGCCTT CTGCAAGATC 
ATCAACTCTT ATTGTGTTTT TCCACGTGAA CTGAAGGAGG TCTTTGCATC ATGGAAGCAG 
CAGTGTCACG CTCGAGGGAA GCAGGACATC AGTCAGCGTC TTATCAGCGC ATCGCTCTTC 
CTGCGCTTTC TGTGTCCTGC CATCATGTCC CCGTCACTCT TTGGTCTCAT GCAGGAATAT 
CCAGATGACC GTACTTCTCG AACACTCACC CTCATTGCCA AAGTCATTCA GAATCTTGCT 
AACTTTGCCA AATTCGGGAA CAAAGAGGAG TACATGGCTT TCATGAATGA CTTTCTAGAG 
CACGAGTGGG CTGGTATGAC CCGTTTTCTG CTTGAGATCT CGAATCTTGA GACAATCTCC 
AACACCCCGG GCTTTGAGGG CTACATTGAC CTGGGCCGGG AGCTGTCAGT GCTTCACTCG 
CTGCTCTGGG AGGTGGTGTC TCAGCTGGAC AAGGGTGACA ATTCCTTCCT GCAGGCGACG 
GTGGCCAAAC TGGGGCCCCT GCCACGTATC CTTGGCGATA TCACCCGCTC GCTGTCCAGC 
CCTACTCCCA TCCAGCAGCA GCTCAGGCGC TTCCAGGACC ACAGCTCTGC TCATGACATC 
AGTGGAAGTG TGTCCTCAGG ACTGCAGAGA ATATTTGAAG ACCCAGCAGA CAGTGAAATG 
AGGAGCATCA AATCACCTGT CCAAGAGCAC ATGGAGGCTT TAGTCAGAGG AAAGCATCCT 
TTACTGGGTC AACAGTCGTC CGCACACAGC ATGAGCTTCT CCGACAAAGA GGAAAGGGAC 
AGTCCGCTCC CGAATGGACG CAGTATATCT TTAATGGACT TGCAGGACTC TTACCTTGCT 
CAGGGGCATC CAGGTCCCAA CTCGCTCAAT GAAGCCTCAT CTAGGTTAGG CAGAGTAGGC 
TCTCAGGCCT CCATCGGCCC TGTCCCTCCT CCACACCTCC ACCAGCCTCC AGTCCACCCG 
AAAGTTCCAC AGCTGAGGGA TAACTTACCC CAGAGTGCAC CACAGGTGCG GCGCCCGATC 
CACCCATCCC TTAGCCAGCA GCGCAGCCTA CAACCGCTGT CTTTCCAAAA CCCTGTTTAC 
CACCTCAGCA ACCTGCACGC ACAATCAACA CACTCAACCC AGTCCACACA CTCTGCCCAG 
TCCCTGCAGC CTGACTCTAG CTCAGAAAAC CTGAGCACAG GGAGCTCTCG GAGCGCCAGT 
CCTAGTGCCT CAGGTGGCCG GGGAGCAACT CCCAGAGCCC GGATACCCTC CACAAGCAGC 
GTGGAGGAGG AATTTAGCCG AAGAAGCATC CAGGGACAGG AGACGCCGCC TCCAACGGCA 
CGCTGGCACC CTCCCCTGGC AGACCAGCAC TCGGGAGCCC AGGTGGTGGC AGTGCCAAGG 
CAGAGTACCA CCGGTACGGC ACATATAGTG AAAGTGGAGC AGCAGAGTCG AGGAGTTGGG 
TTGGTGGCAG CAAATGCTGG AGCACGAACC CCAAGGTCGC TCCCTCACAG CACGTCCATC 
CGCAGCGGCA GCAGCGCCAA CACTGAACCC ATACAGCAGA GCGGTGAACG ATCAAGACAG 
CAGTCCACGT GCTCCAAAGA TGGTTCAGTA CCAGGAGGAC GAGCCAACAA ACAGGTCCAG 
TCACCTGTAG AGTCTGTCAC CATGTCCCCA GTGGAGCGAA CAGCTGCGTG GGTTTTGAAT 
AATGGCCAGT ATGAAGATGA GGAAGAGGAA GGGCAGAGTA AAGACGATGC CAAACATGTG 
GAGAAGTATG AACAGGAAAT CTCGAAGCTA AAGGAGCGTC TGCGGACGTC TAGTCGGCGG 
CTGGAGGAGT ATGAGAGACG GTTACTGGCT CAGGAGCAGC AGATGCAGAA GCTGCTCTTA 
GAATACAAGA CTCGACTGGA GGACAGTGAG GACCGTCTGC GAAGACAACA GGAGGAAAAA 
GACAGCCAGA TGAAGAGTAT TATCTGCAGG CTGATGGCCG TGGAGGAAGA GCTGAAGAGA 
GACCATGCAG AGATGCAGGC AGTCATAGAT GCCAAACAGA AGATAATCGA TGCACAGGAG 
AAGAGGATCA GCTCTCTGGA TGCTGCTAAC TCTCGGCTGA TGAGCGCTCT GACTCAGGTG 
AAGGAGCGCT ACAGCATGCA CAACCTCCGG AACGGCCTGT CACCCACCAA CCCCACCAAA 
CTCTCCATCA CAGAGAACGG GGAGTTCAAG AATAGCAGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasal2 ( XM_005171325.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171382.1 Danio rerio RAS protein activator like 2 (rasal2), transcript variant X5, mRNA.

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CloneID	ODa07241
Clone ID Related Accession (Same CDS sequence)	XM_005171325.4 , XM_005171325.3
Accession Version	XM_005171325.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras GTPase-activating protein nGAP isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171325.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTCA TAGCGGAAGC GTCCGCGGAG CGCTCTCCCA GACGGAGGAG CATATCAGGG 
CTAGGCAGCT CAGAGAAGAA CATTTCCATC GATGGCCCCA ACTCATCTCC CTTCAAAGTG 
CCTGGTTTTT TAAGTAAACG ACTGAAAGGA TCCATCAAAA GGACGAAGAG CCAGACTAAG 
CTGGACCGCA ACACAAGTTT CAGACTCCCA TCGCTGCGAC CAACAGAAAA TGACAGGTCA 
CGAGGCCTGC CCAAATTGAA GGAGTCCTGT TCCCATGAGT CTTTGCTGAG TCCTGGCAGC 
GCTGTTGAAG CTCTGGATCT GAGTATGGAG GAGGATGTCT ACATCAAACC TTTACACAGC 
AGCATCCTAG GACAAGATTT CTGCTTTGAG GTGGCGTACT CAGGTGGAAG TAAGTGCTTC 
AGCTGTTCCT CTGCTGCTGA ACGGGACAAA TGGATGGAGA ACCTCAAAAG AACTGTGCAA 
CCTAATAAGG ATAATTGCCG GCGGGCGGAA AACGTCCTCC GCCTGTGGAT CATTGAGGCA 
AAAGACCTGC CACCAAAAAA GAAATATTTC TGTGAGCTGT GTCTGGATGA CATGCTGTAC 
GCTCGTACCA CCAGCAAAAC CCGCTCCGAC TGCCTGTTCT GGGGGGAACA CTTTGAGTTT 
TCAGGCCTCC CGTCCATGAA AAGCATCACC ATACACATCT ACCGGGATGT GGACAAGAAA 
AAGAAAAAGG ACAAAAACAA CTATGTTGGC CTGGTGAACA TCCCTGTGCA TGGGGTGATG 
GGTAGGCAGT TTGTGGAGAA GTGGTATCCA GTCAGCACTC CCACCACCAG CAAAGCCAAA 
GGTGGAGGCC CCTCCATCCG CATCAAATCC CGCTTTCAGA CCATCTCAAT TCTGCCCATG 
GAGCAGTACA AGGAGTTTGC GGAGTTTGTT ACCAATAACT ATACCATGTT GTGCTCTGTG 
TTGGAGCCGG TCATAAGCGT GAAGAACAAA GAGGAGATGG CCAGCGCTCT GGTGCACATT 
CTGCAGAGCA CGGGACGGGC AAAGGACTTC CTAACGGATT TGGTGATGTC AGAAGTGGAT 
CGCTGTGCTG ACCATGATGT GCTGATCTTT AGAGAAAACA CACTAGCCAC CAAAGCCATT 
GAAGAATACC TCAAACTGGT GGGACAGAAG TACCTACATG ATGCACTAGG GGAGTTTATT 
AAAGCCCTGT ACGAGTCGGA TGAAAATTGT GAAGTAGACC AATCAAAGTG CACCAGCAAC 
GAGCTTCCAG AGCATCAGAG TAACCTGAAG ATGTGCTGCG AACTGGCCTT CTGCAAGATC 
ATCAACTCTT ATTGTGTTTT TCCACGTGAA CTGAAGGAGG TCTTTGCATC ATGGAAGCAG 
CAGTGTCACG CTCGAGGGAA GCAGGACATC AGTCAGCGTC TTATCAGCGC ATCGCTCTTC 
CTGCGCTTTC TGTGTCCTGC CATCATGTCC CCGTCACTCT TTGGTCTCAT GCAGGAATAT 
CCAGATGACC GTACTTCTCG AACACTCACC CTCATTGCCA AAGTCATTCA GAATCTTGCT 
AACTTTGCCA AATTCGGGAA CAAAGAGGAG TACATGGCTT TCATGAATGA CTTTCTAGAG 
CACGAGTGGG CTGGTATGAC CCGTTTTCTG CTTGAGATCT CGAATCTTGA GACAATCTCC 
AACACCCCGG GCTTTGAGGG CTACATTGAC CTGGGCCGGG AGCTGTCAGT GCTTCACTCG 
CTGCTCTGGG AGGTGGTGTC TCAGCTGGAC AAGGGTGACA ATTCCTTCCT GCAGGCGACG 
GTGGCCAAAC TGGGGCCCCT GCCACGTATC CTTGGCGATA TCACCCGCTC GCTGTCCAGC 
CCTACTCCCA TCCAGCAGCA GCTCAGGCGC TTCCAGGACC ACAGCTCTGC TCATGACATC 
AGTGGAAGTG TGTCCTCAGG ACTGCAGAGA ATATTTGAAG ACCCAGCAGA CAGTGAAATG 
AGGAGCATCA AATCACCTGT CCAAGAGCAC ATGGAGGCTT TAGTCAGAGG AAAGCATCCT 
TTACTGGGTC AACAGTCGTC CGCACACAGC ATGAGCTTCT CCGACAAAGA GGAAAGGGAC 
AGTCCGCTCC CGAATGGACG CAGTATATCT TTAATGGACT TGCAGGACTC TTACCTTGCT 
CAGGGGCATC CAGGTCCCAA CTCGCTCAAT GAAGCCTCAT CTAGGTTAGG CAGAGTAGGC 
TCTCAGGCCT CCATCGGCCC TGTCCCTCCT CCACACCTCC ACCAGCCTCC AGTCCACCCG 
AAAGTTCCAC AGCTGAGGGA TAACTTACCC CAGAGTGCAC CACAGGTGCG GCGCCCGATC 
CACCCATCCC TTAGCCAGCA GCGCAGCCTA CAACCGCTGT CTTTCCAAAA CCCTGTTTAC 
CACCTCAGCA ACCTGCACGC ACAATCAACA CACTCAACCC AGTCCACACA CTCTGCCCAG 
TCCCTGCAGC CTGACTCTAG CTCAGAAAAC CTGAGCACAG GGAGCTCTCG GAGCGCCAGT 
CCTAGTGCCT CAGGTGGCCG GGGAGCAACT CCCAGAGCCC GGATACCCTC CACAAGCAGC 
GTGGAGGAGG AATTTAGCCG AAGAAGCATC CAGGGACAGG AGACGCCGCC TCCAACGGCA 
CGCTGGCACC CTCCCCTGGC AGACCAGCAC TCGGGAGCCC AGGTGGTGGC AGTGCCAAGG 
CAGAGTACCA CCGGTACGGC ACATATAGTG AAAGTGGAGC AGCAGAGTCG AGGAGTTGGG 
TTGGTGGCAG CAAATGCTGG AGCACGAACC CCAAGGTCGC TCCCTCACAG CACGTCCATC 
CGCAGCGGCA GCAGCGCCAA CACTGAACCC ATACAGCAGA GCGGTGAACG ATCAAGACAG 
CAGTCCACGT GCTCCAAAGA TGGTTCAGTA CCAGGAGGAC GAGCCAACAA ACAGGTCCAG 
TCACCTGTAG AGTCTGTCAC CATGTCCCCA GTGGAGCGAA CAGCTGCGTG GGTTTTGAAT 
AATGGCCAGT ATGAAGATGA GGAAGAGGAA GGGCAGAGTA AAGACGATGC CAAACATGTG 
GAGAAGTATG AACAGGAAAT CTCGAAGCTA AAGGAGCGTC TGCGGACGTC TAGTCGGCGG 
CTGGAGGAGT ATGAGAGACG GTTACTGGCT CAGGAGCAGC AGATGCAGAA GCTGCTCTTA 
GAATACAAGA CTCGACTGGA GGACAGTGAG GACCGTCTGC GAAGACAACA GGAGGAAAAA 
GACAGCCAGA TGAAGAGTAT TATCTGCAGG CTGATGGCCG TGGAGGAAGA GCTGAAGAGA 
GACCATGCAG AGATGCAGGC AGTCATAGAT GCCAAACAGA AGATAATCGA TGCACAGGAG 
AAGAGGATCA GCTCTCTGGA TGCTGCTAAC TCTCGGCTGA TGAGCGCTCT GACTCAGGTG 
AAGGAGCGCT ACAGCATGCA CAACCTCCGG AACGGCCTGT CACCCACCAA CCCCACCAAA 
CTCTCCATCA CAGAGAACGG GGAGTTCAAG AATAGCAGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171382.1
CDS	124..3648
Translation	MDFIAEASAERSPRRRSISGLGSSEKNISIDGPNSSPFKVPGFLSKRLKGSIKRTKSQTKLDRNTSFRLPSLRPTENDRSRGLPKLKESCSHESLLSPGSAVEALDLSMEEDVYIKPLHSSILGQDFCFEVAYSGGSKCFSCSSAAERDKWMENLKRTVQPNKDNCRRAENVLRLWIIEAKDLPPKKKYFCELCLDDMLYARTTSKTRSDCLFWGEHFEFSGLPSMKSITIHIYRDVDKKKKKDKNNYVGLVNIPVHGVMGRQFVEKWYPVSTPTTSKAKGGGPSIRIKSRFQTISILPMEQYKEFAEFVTNNYTMLCSVLEPVISVKNKEEMASALVHILQSTGRAKDFLTDLVMSEVDRCADHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDQSKCTSNELPEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCHARGKQDISQRLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWAGMTRFLLEISNLETISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKGDNSFLQATVAKLGPLPRILGDITRSLSSPTPIQQQLRRFQDHSSAHDISGSVSSGLQRIFEDPADSEMRSIKSPVQEHMEALVRGKHPLLGQQSSAHSMSFSDKEERDSPLPNGRSISLMDLQDSYLAQGHPGPNSLNEASSRLGRVGSQASIGPVPPPHLHQPPVHPKVPQLRDNLPQSAPQVRRPIHPSLSQQRSLQPLSFQNPVYHLSNLHAQSTHSTQSTHSAQSLQPDSSSENLSTGSSRSASPSASGGRGATPRARIPSTSSVEEEFSRRSIQGQETPPPTARWHPPLADQHSGAQVVAVPRQSTTGTAHIVKVEQQSRGVGLVAANAGARTPRSLPHSTSIRSGSSANTEPIQQSGERSRQQSTCSKDGSVPGGRANKQVQSPVESVTMSPVERTAAWVLNNGQYEDEEEEGQSKDDAKHVEKYEQEISKLKERLRTSSRRLEEYERRLLAQEQQMQKLLLEYKTRLEDSEDRLRRQQEEKDSQMKSIICRLMAVEEELKRDHAEMQAVIDAKQKIIDAQEKRISSLDAANSRLMSALTQVKERYSMHNLRNGLSPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_005171325.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171325.3

ORF Insert Sequence:

ATGGATTTCA TAGCGGAAGC GTCCGCGGAG CGCTCTCCCA GACGGAGGAG CATATCAGGG 
CTAGGCAGCT CAGAGAAGAA CATTTCCATC GATGGCCCCA ACTCATCTCC CTTCAAAGTG 
CCTGGTTTTT TAAGTAAACG ACTGAAAGGA TCCATCAAAA GGACGAAGAG CCAGACTAAG 
CTGGACCGCA ACACAAGTTT CAGACTCCCA TCGCTGCGAC CAACAGAAAA TGACAGGTCA 
CGAGGCCTGC CCAAATTGAA GGAGTCCTGT TCCCATGAGT CTTTGCTGAG TCCTGGCAGC 
GCTGTTGAAG CTCTGGATCT GAGTATGGAG GAGGATGTCT ACATCAAACC TTTACACAGC 
AGCATCCTAG GACAAGATTT CTGCTTTGAG GTGGCGTACT CAGGTGGAAG TAAGTGCTTC 
AGCTGTTCCT CTGCTGCTGA ACGGGACAAA TGGATGGAGA ACCTCAAAAG AACTGTGCAA 
CCTAATAAGG ATAATTGCCG GCGGGCGGAA AACGTCCTCC GCCTGTGGAT CATTGAGGCA 
AAAGACCTGC CACCAAAAAA GAAATATTTC TGTGAGCTGT GTCTGGATGA CATGCTGTAC 
GCTCGTACCA CCAGCAAAAC CCGCTCCGAC TGCCTGTTCT GGGGGGAACA CTTTGAGTTT 
TCAGGCCTCC CGTCCATGAA AAGCATCACC ATACACATCT ACCGGGATGT GGACAAGAAA 
AAGAAAAAGG ACAAAAACAA CTATGTTGGC CTGGTGAACA TCCCTGTGCA TGGGGTGATG 
GGTAGGCAGT TTGTGGAGAA GTGGTATCCA GTCAGCACTC CCACCACCAG CAAAGCCAAA 
GGTGGAGGCC CCTCCATCCG CATCAAATCC CGCTTTCAGA CCATCTCAAT TCTGCCCATG 
GAGCAGTACA AGGAGTTTGC GGAGTTTGTT ACCAATAACT ATACCATGTT GTGCTCTGTG 
TTGGAGCCGG TCATAAGCGT GAAGAACAAA GAGGAGATGG CCAGCGCTCT GGTGCACATT 
CTGCAGAGCA CGGGACGGGC AAAGGACTTC CTAACGGATT TGGTGATGTC AGAAGTGGAT 
CGCTGTGCTG ACCATGATGT GCTGATCTTT AGAGAAAACA CACTAGCCAC CAAAGCCATT 
GAAGAATACC TCAAACTGGT GGGACAGAAG TACCTACATG ATGCACTAGG GGAGTTTATT 
AAAGCCCTGT ACGAGTCGGA TGAAAATTGT GAAGTAGACC AATCAAAGTG CACCAGCAAC 
GAGCTTCCAG AGCATCAGAG TAACCTGAAG ATGTGCTGCG AACTGGCCTT CTGCAAGATC 
ATCAACTCTT ATTGTGTTTT TCCACGTGAA CTGAAGGAGG TCTTTGCATC ATGGAAGCAG 
CAGTGTCACG CTCGAGGGAA GCAGGACATC AGTCAGCGTC TTATCAGCGC ATCGCTCTTC 
CTGCGCTTTC TGTGTCCTGC CATCATGTCC CCGTCACTCT TTGGTCTCAT GCAGGAATAT 
CCAGATGACC GTACTTCTCG AACACTCACC CTCATTGCCA AAGTCATTCA GAATCTTGCT 
AACTTTGCCA AATTCGGGAA CAAAGAGGAG TACATGGCTT TCATGAATGA CTTTCTAGAG 
CACGAGTGGG CTGGTATGAC CCGTTTTCTG CTTGAGATCT CGAATCTTGA GACAATCTCC 
AACACCCCGG GCTTTGAGGG CTACATTGAC CTGGGCCGGG AGCTGTCAGT GCTTCACTCG 
CTGCTCTGGG AGGTGGTGTC TCAGCTGGAC AAGGGTGACA ATTCCTTCCT GCAGGCGACG 
GTGGCCAAAC TGGGGCCCCT GCCACGTATC CTTGGCGATA TCACCCGCTC GCTGTCCAGC 
CCTACTCCCA TCCAGCAGCA GCTCAGGCGC TTCCAGGACC ACAGCTCTGC TCATGACATC 
AGTGGAAGTG TGTCCTCAGG ACTGCAGAGA ATATTTGAAG ACCCAGCAGA CAGTGAAATG 
AGGAGCATCA AATCACCTGT CCAAGAGCAC ATGGAGGCTT TAGTCAGAGG AAAGCATCCT 
TTACTGGGTC AACAGTCGTC CGCACACAGC ATGAGCTTCT CCGACAAAGA GGAAAGGGAC 
AGTCCGCTCC CGAATGGACG CAGTATATCT TTAATGGACT TGCAGGACTC TTACCTTGCT 
CAGGGGCATC CAGGTCCCAA CTCGCTCAAT GAAGCCTCAT CTAGGTTAGG CAGAGTAGGC 
TCTCAGGCCT CCATCGGCCC TGTCCCTCCT CCACACCTCC ACCAGCCTCC AGTCCACCCG 
AAAGTTCCAC AGCTGAGGGA TAACTTACCC CAGAGTGCAC CACAGGTGCG GCGCCCGATC 
CACCCATCCC TTAGCCAGCA GCGCAGCCTA CAACCGCTGT CTTTCCAAAA CCCTGTTTAC 
CACCTCAGCA ACCTGCACGC ACAATCAACA CACTCAACCC AGTCCACACA CTCTGCCCAG 
TCCCTGCAGC CTGACTCTAG CTCAGAAAAC CTGAGCACAG GGAGCTCTCG GAGCGCCAGT 
CCTAGTGCCT CAGGTGGCCG GGGAGCAACT CCCAGAGCCC GGATACCCTC CACAAGCAGC 
GTGGAGGAGG AATTTAGCCG AAGAAGCATC CAGGGACAGG AGACGCCGCC TCCAACGGCA 
CGCTGGCACC CTCCCCTGGC AGACCAGCAC TCGGGAGCCC AGGTGGTGGC AGTGCCAAGG 
CAGAGTACCA CCGGTACGGC ACATATAGTG AAAGTGGAGC AGCAGAGTCG AGGAGTTGGG 
TTGGTGGCAG CAAATGCTGG AGCACGAACC CCAAGGTCGC TCCCTCACAG CACGTCCATC 
CGCAGCGGCA GCAGCGCCAA CACTGAACCC ATACAGCAGA GCGGTGAACG ATCAAGACAG 
CAGTCCACGT GCTCCAAAGA TGGTTCAGTA CCAGGAGGAC GAGCCAACAA ACAGGTCCAG 
TCACCTGTAG AGTCTGTCAC CATGTCCCCA GTGGAGCGAA CAGCTGCGTG GGTTTTGAAT 
AATGGCCAGT ATGAAGATGA GGAAGAGGAA GGGCAGAGTA AAGACGATGC CAAACATGTG 
GAGAAGTATG AACAGGAAAT CTCGAAGCTA AAGGAGCGTC TGCGGACGTC TAGTCGGCGG 
CTGGAGGAGT ATGAGAGACG GTTACTGGCT CAGGAGCAGC AGATGCAGAA GCTGCTCTTA 
GAATACAAGA CTCGACTGGA GGACAGTGAG GACCGTCTGC GAAGACAACA GGAGGAAAAA 
GACAGCCAGA TGAAGAGTAT TATCTGCAGG CTGATGGCCG TGGAGGAAGA GCTGAAGAGA 
GACCATGCAG AGATGCAGGC AGTCATAGAT GCCAAACAGA AGATAATCGA TGCACAGGAG 
AAGAGGATCA GCTCTCTGGA TGCTGCTAAC TCTCGGCTGA TGAGCGCTCT GACTCAGGTG 
AAGGAGCGCT ACAGCATGCA CAACCTCCGG AACGGCCTGT CACCCACCAA CCCCACCAAA 
CTCTCCATCA CAGAGAACGG GGAGTTCAAG AATAGCAGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasal2 ( XM_009298648.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296923.1 Danio rerio RAS protein activator like 2 (rasal2), transcript variant X6, mRNA.

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CloneID	ODa46832
Clone ID Related Accession (Same CDS sequence)	XM_009298648.2
Accession Version	XM_009298648.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras GTPase-activating protein nGAP isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298648.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGG GATACTCCTT CAAGTGGATC TTTAGTTGTT TTTCGTCACG AGGCCTGCCC 
AAATTGAAGG AGTCCTGTTC CCATGAGTCT TTGCTGAGTC CTGGCAGCGC TGTTGAAGCT 
CTGGATCTGA GTATGGAGGA GGATGTCTAC ATCAAACCTT TACACAGCAG CATCCTAGGA 
CAAGATTTCT GCTTTGAGGT GGCGTACTCA GGTGGAAGTA AGTGCTTCAG CTGTTCCTCT 
GCTGCTGAAC GGGACAAATG GATGGAGAAC CTCAAAAGAA CTGTGCAACC TAATAAGGAT 
AATTGCCGGC GGGCGGAAAA CGTCCTCCGC CTGTGGATCA TTGAGGCAAA AGACCTGCCA 
CCAAAAAAGA AATATTTCTG TGAGCTGTGT CTGGATGACA TGCTGTACGC TCGTACCACC 
AGCAAAACCC GCTCCGACTG CCTGTTCTGG GGGGAACACT TTGAGTTTTC AGGCCTCCCG 
TCCATGAAAA GCATCACCAT ACACATCTAC CGGGATGTGG ACAAGAAAAA GAAAAAGGAC 
AAAAACAACT ATGTTGGCCT GGTGAACATC CCTGTGCATG GGGTGATGGG TAGGCAGTTT 
GTGGAGAAGT GGTATCCAGT CAGCACTCCC ACCACCAGCA AAGCCAAAGG TGGAGGCCCC 
TCCATCCGCA TCAAATCCCG CTTTCAGACC ATCTCAATTC TGCCCATGGA GCAGTACAAG 
GAGTTTGCGG AGTTTGTTAC CAATAACTAT ACCATGTTGT GCTCTGTGTT GGAGCCGGTC 
ATAAGCGTGA AGAACAAAGA GGAGATGGCC AGCGCTCTGG TGCACATTCT GCAGAGCACG 
GGACGGGCAA AGGACTTCCT AACGGATTTG GTGATGTCAG AAGTGGATCG CTGTGCTGAC 
CATGATGTGC TGATCTTTAG AGAAAACACA CTAGCCACCA AAGCCATTGA AGAATACCTC 
AAACTGGTGG GACAGAAGTA CCTACATGAT GCACTAGGGG AGTTTATTAA AGCCCTGTAC 
GAGTCGGATG AAAATTGTGA AGTAGACCAA TCAAAGTGCA CCAGCAACGA GCTTCCAGAG 
CATCAGAGTA ACCTGAAGAT GTGCTGCGAA CTGGCCTTCT GCAAGATCAT CAACTCTTAT 
TGTGTTTTTC CACGTGAACT GAAGGAGGTC TTTGCATCAT GGAAGCAGCA GTGTCACGCT 
CGAGGGAAGC AGGACATCAG TCAGCGTCTT ATCAGCGCAT CGCTCTTCCT GCGCTTTCTG 
TGTCCTGCCA TCATGTCCCC GTCACTCTTT GGTCTCATGC AGGAATATCC AGATGACCGT 
ACTTCTCGAA CACTCACCCT CATTGCCAAA GTCATTCAGA ATCTTGCTAA CTTTGCCAAA 
TTCGGGAACA AAGAGGAGTA CATGGCTTTC ATGAATGACT TTCTAGAGCA CGAGTGGGCT 
GGTATGACCC GTTTTCTGCT TGAGATCTCG AATCTTGAGA CAATCTCCAA CACCCCGGGC 
TTTGAGGGCT ACATTGACCT GGGCCGGGAG CTGTCAGTGC TTCACTCGCT GCTCTGGGAG 
GTGGTGTCTC AGCTGGACAA GGGTGACAAT TCCTTCCTGC AGGCGACGGT GGCCAAACTG 
GGGCCCCTGC CACGTATCCT TGGCGATATC ACCCGCTCGC TGTCCAGCCC TACTCCCATC 
CAGCAGCAGC TCAGGCGCTT CCAGGACCAC AGCTCTGCTC ATGACATCAG TGGAAGTGTG 
TCCTCAGGAC TGCAGAGAAT ATTTGAAGAC CCAGCAGACA GTGAAATGAG GAGCATCAAA 
TCACCTGTCC AAGAGCACAT GGAGGCTTTA GTCAGAGGAA AGCATCCTTT ACTGGGTCAA 
CAGTCGTCCG CACACAGCAT GAGCTTCTCC GACAAAGAGG AAAGGGACAG TCCGCTCCCG 
AATGGACGCA GTATATCTTT AATGGACTTG CAGGACTCTT ACCTTGCTCA GGGGCATCCA 
GGTCCCAACT CGCTCAATGA AGCCTCATCT AGGTTAGGCA GAGTAGGCTC TCAGGCCTCC 
ATCGGCCCTG TCCCTCCTCC ACACCTCCAC CAGCCTCCAG TCCACCCGAA AGTTCCACAG 
CTGAGGGATA ACTTACCCCA GAGTGCACCA CAGGTGCGGC GCCCGATCCA CCCATCCCTT 
AGCCAGCAGC GCAGCCTACA ACCGCTGTCT TTCCAAAACC CTGTTTACCA CCTCAGCAAC 
CTGCACGCAC AATCAACACA CTCAACCCAG TCCACACACT CTGCCCAGTC CCTGCAGCCT 
GACTCTAGCT CAGAAAACCT GAGCACAGGG AGCTCTCGGA GCGCCAGTCC TAGTGCCTCA 
GGTGGCCGGG GAGCAACTCC CAGAGCCCGG ATACCCTCCA CAAGCAGCGT GGAGGAGGAA 
TTTAGCCGAA GAAGCATCCA GGGACAGGAG ACGCCGCCTC CAACGGCACG CTGGCACCCT 
CCCCTGGCAG ACCAGCACTC GGGAGCCCAG GTGGTGGCAG TGCCAAGGCA GAGTACCACC 
GGTACGGCAC ATATAGTGAA AGTGGAGCAG CAGAGTCGAG GAGTTGGGTT GGTGGCAGCA 
AATGCTGGAG CACGAACCCC AAGGTCGCTC CCTCACAGCA CGTCCATCCG CAGCGGCAGC 
AGCGCCAACA CTGAACCCAT ACAGCAGAGC GGTGAACGAT CAAGACAGCA GTCCACGTGC 
TCCAAAGATG GTTCAGTACC AGGAGGACGA GCCAACAAAC AGGTCCAGTC ACCTGTAGAG 
TCTGTCACCA TGTCCCCAGT GGAGCGAACA GCTGCGTGGG TTTTGAATAA TGGCCAGTAT 
GAAGATGAGG AAGAGGAAGG GCAGAGTAAA GACGATGCCA AACATGTGGA GAAGTATGAA 
CAGGAAATCT CGAAGCTAAA GGAGCGTCTG CGGACGTCTA GTCGGCGGCT GGAGGAGTAT 
GAGAGACGGT TACTGGCTCA GGAGCAGCAG ATGCAGAAGC TGCTCTTAGA ATACAAGACT 
CGACTGGAGG ACAGTGAGGA CCGTCTGCGA AGACAACAGG AGGAAAAAGA CAGCCAGATG 
AAGAGTATTA TCTGCAGGCT GATGGCCGTG GAGGAAGAGC TGAAGAGAGA CCATGCAGAG 
ATGCAGGCAG TCATAGATGC CAAACAGAAG ATAATCGATG CACAGGAGAA GAGGATCAGC 
TCTCTGGATG CTGCTAACTC TCGGCTGATG AGCGCTCTGA CTCAGGTGAA GGAGCGCTAC 
AGCATGCACA ACCTCCGGAA CGGCCTGTCA CCCACCAACC CCACCAAACT CTCCATCACA 
GAGAACGGGG AGTTCAAGAA TAGCAGCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296923.1
CDS	16..3348
Translation	MLLGYSFKWIFSCFSSRGLPKLKESCSHESLLSPGSAVEALDLSMEEDVYIKPLHSSILGQDFCFEVAYSGGSKCFSCSSAAERDKWMENLKRTVQPNKDNCRRAENVLRLWIIEAKDLPPKKKYFCELCLDDMLYARTTSKTRSDCLFWGEHFEFSGLPSMKSITIHIYRDVDKKKKKDKNNYVGLVNIPVHGVMGRQFVEKWYPVSTPTTSKAKGGGPSIRIKSRFQTISILPMEQYKEFAEFVTNNYTMLCSVLEPVISVKNKEEMASALVHILQSTGRAKDFLTDLVMSEVDRCADHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDQSKCTSNELPEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCHARGKQDISQRLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWAGMTRFLLEISNLETISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKGDNSFLQATVAKLGPLPRILGDITRSLSSPTPIQQQLRRFQDHSSAHDISGSVSSGLQRIFEDPADSEMRSIKSPVQEHMEALVRGKHPLLGQQSSAHSMSFSDKEERDSPLPNGRSISLMDLQDSYLAQGHPGPNSLNEASSRLGRVGSQASIGPVPPPHLHQPPVHPKVPQLRDNLPQSAPQVRRPIHPSLSQQRSLQPLSFQNPVYHLSNLHAQSTHSTQSTHSAQSLQPDSSSENLSTGSSRSASPSASGGRGATPRARIPSTSSVEEEFSRRSIQGQETPPPTARWHPPLADQHSGAQVVAVPRQSTTGTAHIVKVEQQSRGVGLVAANAGARTPRSLPHSTSIRSGSSANTEPIQQSGERSRQQSTCSKDGSVPGGRANKQVQSPVESVTMSPVERTAAWVLNNGQYEDEEEEGQSKDDAKHVEKYEQEISKLKERLRTSSRRLEEYERRLLAQEQQMQKLLLEYKTRLEDSEDRLRRQQEEKDSQMKSIICRLMAVEEELKRDHAEMQAVIDAKQKIIDAQEKRISSLDAANSRLMSALTQVKERYSMHNLRNGLSPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_009298648.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298648.2

ORF Insert Sequence:

ATGCTCTTGG GATACTCCTT CAAGTGGATC TTTAGTTGTT TTTCGTCACG AGGCCTGCCC 
AAATTGAAGG AGTCCTGTTC CCATGAGTCT TTGCTGAGTC CTGGCAGCGC TGTTGAAGCT 
CTGGATCTGA GTATGGAGGA GGATGTCTAC ATCAAACCTT TACACAGCAG CATCCTAGGA 
CAAGATTTCT GCTTTGAGGT GGCGTACTCA GGTGGAAGTA AGTGCTTCAG CTGTTCCTCT 
GCTGCTGAAC GGGACAAATG GATGGAGAAC CTCAAAAGAA CTGTGCAACC TAATAAGGAT 
AATTGCCGGC GGGCGGAAAA CGTCCTCCGC CTGTGGATCA TTGAGGCAAA AGACCTGCCA 
CCAAAAAAGA AATATTTCTG TGAGCTGTGT CTGGATGACA TGCTGTACGC TCGTACCACC 
AGCAAAACCC GCTCCGACTG CCTGTTCTGG GGGGAACACT TTGAGTTTTC AGGCCTCCCG 
TCCATGAAAA GCATCACCAT ACACATCTAC CGGGATGTGG ACAAGAAAAA GAAAAAGGAC 
AAAAACAACT ATGTTGGCCT GGTGAACATC CCTGTGCATG GGGTGATGGG TAGGCAGTTT 
GTGGAGAAGT GGTATCCAGT CAGCACTCCC ACCACCAGCA AAGCCAAAGG TGGAGGCCCC 
TCCATCCGCA TCAAATCCCG CTTTCAGACC ATCTCAATTC TGCCCATGGA GCAGTACAAG 
GAGTTTGCGG AGTTTGTTAC CAATAACTAT ACCATGTTGT GCTCTGTGTT GGAGCCGGTC 
ATAAGCGTGA AGAACAAAGA GGAGATGGCC AGCGCTCTGG TGCACATTCT GCAGAGCACG 
GGACGGGCAA AGGACTTCCT AACGGATTTG GTGATGTCAG AAGTGGATCG CTGTGCTGAC 
CATGATGTGC TGATCTTTAG AGAAAACACA CTAGCCACCA AAGCCATTGA AGAATACCTC 
AAACTGGTGG GACAGAAGTA CCTACATGAT GCACTAGGGG AGTTTATTAA AGCCCTGTAC 
GAGTCGGATG AAAATTGTGA AGTAGACCAA TCAAAGTGCA CCAGCAACGA GCTTCCAGAG 
CATCAGAGTA ACCTGAAGAT GTGCTGCGAA CTGGCCTTCT GCAAGATCAT CAACTCTTAT 
TGTGTTTTTC CACGTGAACT GAAGGAGGTC TTTGCATCAT GGAAGCAGCA GTGTCACGCT 
CGAGGGAAGC AGGACATCAG TCAGCGTCTT ATCAGCGCAT CGCTCTTCCT GCGCTTTCTG 
TGTCCTGCCA TCATGTCCCC GTCACTCTTT GGTCTCATGC AGGAATATCC AGATGACCGT 
ACTTCTCGAA CACTCACCCT CATTGCCAAA GTCATTCAGA ATCTTGCTAA CTTTGCCAAA 
TTCGGGAACA AAGAGGAGTA CATGGCTTTC ATGAATGACT TTCTAGAGCA CGAGTGGGCT 
GGTATGACCC GTTTTCTGCT TGAGATCTCG AATCTTGAGA CAATCTCCAA CACCCCGGGC 
TTTGAGGGCT ACATTGACCT GGGCCGGGAG CTGTCAGTGC TTCACTCGCT GCTCTGGGAG 
GTGGTGTCTC AGCTGGACAA GGGTGACAAT TCCTTCCTGC AGGCGACGGT GGCCAAACTG 
GGGCCCCTGC CACGTATCCT TGGCGATATC ACCCGCTCGC TGTCCAGCCC TACTCCCATC 
CAGCAGCAGC TCAGGCGCTT CCAGGACCAC AGCTCTGCTC ATGACATCAG TGGAAGTGTG 
TCCTCAGGAC TGCAGAGAAT ATTTGAAGAC CCAGCAGACA GTGAAATGAG GAGCATCAAA 
TCACCTGTCC AAGAGCACAT GGAGGCTTTA GTCAGAGGAA AGCATCCTTT ACTGGGTCAA 
CAGTCGTCCG CACACAGCAT GAGCTTCTCC GACAAAGAGG AAAGGGACAG TCCGCTCCCG 
AATGGACGCA GTATATCTTT AATGGACTTG CAGGACTCTT ACCTTGCTCA GGGGCATCCA 
GGTCCCAACT CGCTCAATGA AGCCTCATCT AGGTTAGGCA GAGTAGGCTC TCAGGCCTCC 
ATCGGCCCTG TCCCTCCTCC ACACCTCCAC CAGCCTCCAG TCCACCCGAA AGTTCCACAG 
CTGAGGGATA ACTTACCCCA GAGTGCACCA CAGGTGCGGC GCCCGATCCA CCCATCCCTT 
AGCCAGCAGC GCAGCCTACA ACCGCTGTCT TTCCAAAACC CTGTTTACCA CCTCAGCAAC 
CTGCACGCAC AATCAACACA CTCAACCCAG TCCACACACT CTGCCCAGTC CCTGCAGCCT 
GACTCTAGCT CAGAAAACCT GAGCACAGGG AGCTCTCGGA GCGCCAGTCC TAGTGCCTCA 
GGTGGCCGGG GAGCAACTCC CAGAGCCCGG ATACCCTCCA CAAGCAGCGT GGAGGAGGAA 
TTTAGCCGAA GAAGCATCCA GGGACAGGAG ACGCCGCCTC CAACGGCACG CTGGCACCCT 
CCCCTGGCAG ACCAGCACTC GGGAGCCCAG GTGGTGGCAG TGCCAAGGCA GAGTACCACC 
GGTACGGCAC ATATAGTGAA AGTGGAGCAG CAGAGTCGAG GAGTTGGGTT GGTGGCAGCA 
AATGCTGGAG CACGAACCCC AAGGTCGCTC CCTCACAGCA CGTCCATCCG CAGCGGCAGC 
AGCGCCAACA CTGAACCCAT ACAGCAGAGC GGTGAACGAT CAAGACAGCA GTCCACGTGC 
TCCAAAGATG GTTCAGTACC AGGAGGACGA GCCAACAAAC AGGTCCAGTC ACCTGTAGAG 
TCTGTCACCA TGTCCCCAGT GGAGCGAACA GCTGCGTGGG TTTTGAATAA TGGCCAGTAT 
GAAGATGAGG AAGAGGAAGG GCAGAGTAAA GACGATGCCA AACATGTGGA GAAGTATGAA 
CAGGAAATCT CGAAGCTAAA GGAGCGTCTG CGGACGTCTA GTCGGCGGCT GGAGGAGTAT 
GAGAGACGGT TACTGGCTCA GGAGCAGCAG ATGCAGAAGC TGCTCTTAGA ATACAAGACT 
CGACTGGAGG ACAGTGAGGA CCGTCTGCGA AGACAACAGG AGGAAAAAGA CAGCCAGATG 
AAGAGTATTA TCTGCAGGCT GATGGCCGTG GAGGAAGAGC TGAAGAGAGA CCATGCAGAG 
ATGCAGGCAG TCATAGATGC CAAACAGAAG ATAATCGATG CACAGGAGAA GAGGATCAGC 
TCTCTGGATG CTGCTAACTC TCGGCTGATG AGCGCTCTGA CTCAGGTGAA GGAGCGCTAC 
AGCATGCACA ACCTCCGGAA CGGCCTGTCA CCCACCAACC CCACCAAACT CTCCATCACA 
GAGAACGGGG AGTTCAAGAA TAGCAGCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasal2 ( XM_005171321.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171378.1 Danio rerio RAS protein activator like 2 (rasal2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa07236
Clone ID Related Accession (Same CDS sequence)	XM_005171321.3 , XM_005171321.4
Accession Version	XM_005171321.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras GTPase-activating protein nGAP isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171321.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTTAA CACGATTGGC CACACTGGAG GAACAAACGG GATCGAACAG GAGCCCATCG 
CGCTCTTCTT TTGGCAAGGA TAGTGTTCGT CGCTGGCGTC TTCTCAACAG GCCTTGGCTG 
AAGCACCGTT CAAATTCAGC TCCCGAGGAG CCCTTGGACA TGCATGACAG AAGGATCCTG 
GTTAGACAGG ACATGCGCAC ACAAAGCTTG ACATGGTCCA GAAGCAGCAG TACTAGGCCA 
TCCAATCCCC CTATCTCTAG GGTCCACAGT CTACCAGACC CTCAGCGGGT CACCAGCCGC 
GGTGTCCTCT TGGAGCATGC TAATCTTCAG AAGGACTGGT TATCTCGCAG CTCTTCGCCT 
TTTTTTAAGT CCACATCCGA ACTGGGGAGC GAAATTCACA CTGGGGCTCC ACCGCTCAGA 
CGGGTGTCAA GCTCCCATAT GTTTTCAGGG ATTCTGCTGT CCATGTCAAG ACGAATTAGC 
CGGATTTTAA ATACCTCTCC TGAGCCAGCA GTGGGAGAAC TTTCAGAAGA CGATGTGAAG 
GGTCCCGCCA GTCACCACTT CTCCTGCGGA CAGTCGCCTT TCACCGAGCC CTGCGTTTGG 
GAGAGGAAAT ACTGCATCCT GACTGATAGC CAGCTCATCC TGCTCAACAA GGAGGAAGAG 
ATGCCGTCAG AGGTGCATGA AAGCCCCTCA GCATCCTCTT CGAAGGGCCG CAGTTTGAGG 
CGGACAGTCA GTGTGCCTTC TGAAGGACCG TTCCCAGACT TCCCACCAGA GGGAGCCTCC 
ATGATAGCGG AAGCGTCCGC GGAGCGCTCT CCCAGACGGA GGAGCATATC AGGGCTAGGC 
AGCTCAGAGA AGAACATTTC CATCGATGGC CCCAACTCAT CTCCCTTCAA AGTGCCTGGT 
TTTTTAAGTA AACGACTGAA AGGATCCATC AAAAGGACGA AGAGCCAGAC TAAGCTGGAC 
CGCAACACAA GTTTCAGACT CCCATCGCTG CGACCAACAG AAAATGACAG GTCACGAGGC 
CTGCCCAAAT TGAAGGAGTC CTGTTCCCAT GAGTCTTTGC TGAGTCCTGG CAGCGCTGTT 
GAAGCTCTGG ATCTGAGTAT GGAGGAGGAT GTCTACATCA AACCTTTACA CAGCAGCATC 
CTAGGACAAG ATTTCTGCTT TGAGGTGGCG TACTCAGGTG GAAGTAAGTG CTTCAGCTGT 
TCCTCTGCTG CTGAACGGGA CAAATGGATG GAGAACCTCA AAAGAACTGT GCAACCTAAT 
AAGGATAATT GCCGGCGGGC GGAAAACGTC CTCCGCCTGT GGATCATTGA GGCAAAAGAC 
CTGCCACCAA AAAAGAAATA TTTCTGTGAG CTGTGTCTGG ATGACATGCT GTACGCTCGT 
ACCACCAGCA AAACCCGCTC CGACTGCCTG TTCTGGGGGG AACACTTTGA GTTTTCAGGC 
CTCCCGTCCA TGAAAAGCAT CACCATACAC ATCTACCGGG ATGTGGACAA GAAAAAGAAA 
AAGGACAAAA ACAACTATGT TGGCCTGGTG AACATCCCTG TGCATGGGGT GATGGGTAGG 
CAGTTTGTGG AGAAGTGGTA TCCAGTCAGC ACTCCCACCA CCAGCAAAGC CAAAGGTGGA 
GGCCCCTCCA TCCGCATCAA ATCCCGCTTT CAGACCATCT CAATTCTGCC CATGGAGCAG 
TACAAGGAGT TTGCGGAGTT TGTTACCAAT AACTATACCA TGTTGTGCTC TGTGTTGGAG 
CCGGTCATAA GCGTGAAGAA CAAAGAGGAG ATGGCCAGCG CTCTGGTGCA CATTCTGCAG 
AGCACGGGAC GGGCAAAGGA CTTCCTAACG GATTTGGTGA TGTCAGAAGT GGATCGCTGT 
GCTGACCATG ATGTGCTGAT CTTTAGAGAA AACACACTAG CCACCAAAGC CATTGAAGAA 
TACCTCAAAC TGGTGGGACA GAAGTACCTA CATGATGCAC TAGGGGAGTT TATTAAAGCC 
CTGTACGAGT CGGATGAAAA TTGTGAAGTA GACCAATCAA AGTGCACCAG CAACGAGCTT 
CCAGAGCATC AGAGTAACCT GAAGATGTGC TGCGAACTGG CCTTCTGCAA GATCATCAAC 
TCTTATTGTG TTTTTCCACG TGAACTGAAG GAGGTCTTTG CATCATGGAA GCAGCAGTGT 
CACGCTCGAG GGAAGCAGGA CATCAGTCAG CGTCTTATCA GCGCATCGCT CTTCCTGCGC 
TTTCTGTGTC CTGCCATCAT GTCCCCGTCA CTCTTTGGTC TCATGCAGGA ATATCCAGAT 
GACCGTACTT CTCGAACACT CACCCTCATT GCCAAAGTCA TTCAGAATCT TGCTAACTTT 
GCCAAATTCG GGAACAAAGA GGAGTACATG GCTTTCATGA ATGACTTTCT AGAGCACGAG 
TGGGCTGGTA TGACCCGTTT TCTGCTTGAG ATCTCGAATC TTGAGACAAT CTCCAACACC 
CCGGGCTTTG AGGGCTACAT TGACCTGGGC CGGGAGCTGT CAGTGCTTCA CTCGCTGCTC 
TGGGAGGTGG TGTCTCAGCT GGACAAGGGT GACAATTCCT TCCTGCAGGC GACGGTGGCC 
AAACTGGGGC CCCTGCCACG TATCCTTGGC GATATCACCC GCTCGCTGTC CAGCCCTACT 
CCCATCCAGC AGCAGCTCAG GCGCTTCCAG GACCACAGCT CTGCTCATGA CATCAGTGGA 
AGTGTGTCCT CAGGACTGCA GAGAATATTT GAAGACCCAG CAGACAGTGA AATGAGGAGC 
ATCAAATCAC CTGTCCAAGA GCACATGGAG GCTTTAGTCA GAGGAAAGCA TCCTTTACTG 
GGTCAACAGT CGTCCGCACA CAGCATGAGC TTCTCCGACA AAGAGGAAAG GGACAGTCCG 
CTCCCGAATG GACGCAGTAT ATCTTTAATG GACTTGCAGG ACTCTTACCT TGCTCAGGGG 
CATCCAGGTC CCAACTCGCT CAATGAAGCC TCATCTAGGT TAGGCAGAGT AGGCTCTCAG 
GCCTCCATCG GCCCTGTCCC TCCTCCACAC CTCCACCAGC CTCCAGTCCA CCCGAAAGTT 
CCACAGCTGA GGGATAACTT ACCCCAGAGT GCACCACAGG TGCGGCGCCC GATCCACCCA 
TCCCTTAGCC AGCAGCGCAG CCTACAACCG CTGTCTTTCC AAAACCCTGT TTACCACCTC 
AGCAACCTGC ACGCACAATC AACACACTCA ACCCAGTCCA CACACTCTGC CCAGTCCCTG 
CAGCCTGACT CTAGCTCAGA AAACCTGAGC ACAGGGAGCT CTCGGAGCGC CAGTCCTAGT 
GCCTCAGGTG GCCGGGGAGC AACTCCCAGA GCCCGGATAC CCTCCACAAG CAGCGTGGAG 
GAGGAATTTA GCCGAAGAAG CATCCAGGGA CAGGAGACGC CGCCTCCAAC GGCACGCTGG 
CACCCTCCCC TGGCAGACCA GCACTCGGGA GCCCAGGTGG TGGCAGTGCC AAGGCAGAGT 
ACCACCGGTA CGGCACATAT AGTGAAAGTG GAGCAGCAGA GTCGAGGAGT TGGGTTGGTG 
GCAGCAAATG CTGGAGCACG AACCCCAAGG TCGCTCCCTC ACAGCACGTC CATCCGCAGC 
GGCAGCAGCG CCAACACTGA ACCCATACAG CAGAGCGGTG AACGATCAAG ACAGCAGTCC 
ACGTGCTCCA AAGATGGTTC AGTACCAGGA GGACGAGCCA ACAAACAGGT CCAGTCACCT 
GTAGAGTCTG TCACCATGTC CCCAGTGGAG CGAACAGCTG CGTGGGTTTT GAATAATGGC 
CAGTATGAAG ATGAGGAAGA GGAAGGGCAG AGTAAAGACG ATGCCAAACA TGTGGAGAAG 
TATGAACAGG AAATCTCGAA GCTAAAGGAG CGTCTGCGGA CGTCTAGTCG GCGGCTGGAG 
GAGTATGAGA GACGGTTACT GGCTCAGGAG CAGCAGATGC AGAAGCTGCT CTTAGAATAC 
AAGACTCGAC TGGAGGACAG TGAGGACCGT CTGCGAAGAC AACAGGAGGA AAAAGACAGC 
CAGATGAAGA GTATTATCTG CAGGCTGATG GCCGTGGAGG AAGAGCTGAA GAGAGACCAT 
GCAGAGATGC AGGCAGTCAT AGATGCCAAA CAGAAGATAA TCGATGCACA GGAGAAGAGG 
ATCAGCTCTC TGGATGCTGC TAACTCTCGG CTGATGAGCG CTCTGACTCA GGTGAAGGAG 
CGCTACAGCA TGCACAACCT CCGGAACGGC CTGTCACCCA CCAACCCCAC CAAACTCTCC 
ATCACAGAGA ACGGGGAGTT CAAGAATAGC AGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171378.1
CDS	16..4314
Translation	MSLTRLATLEEQTGSNRSPSRSSFGKDSVRRWRLLNRPWLKHRSNSAPEEPLDMHDRRILVRQDMRTQSLTWSRSSSTRPSNPPISRVHSLPDPQRVTSRGVLLEHANLQKDWLSRSSSPFFKSTSELGSEIHTGAPPLRRVSSSHMFSGILLSMSRRISRILNTSPEPAVGELSEDDVKGPASHHFSCGQSPFTEPCVWERKYCILTDSQLILLNKEEEMPSEVHESPSASSSKGRSLRRTVSVPSEGPFPDFPPEGASMIAEASAERSPRRRSISGLGSSEKNISIDGPNSSPFKVPGFLSKRLKGSIKRTKSQTKLDRNTSFRLPSLRPTENDRSRGLPKLKESCSHESLLSPGSAVEALDLSMEEDVYIKPLHSSILGQDFCFEVAYSGGSKCFSCSSAAERDKWMENLKRTVQPNKDNCRRAENVLRLWIIEAKDLPPKKKYFCELCLDDMLYARTTSKTRSDCLFWGEHFEFSGLPSMKSITIHIYRDVDKKKKKDKNNYVGLVNIPVHGVMGRQFVEKWYPVSTPTTSKAKGGGPSIRIKSRFQTISILPMEQYKEFAEFVTNNYTMLCSVLEPVISVKNKEEMASALVHILQSTGRAKDFLTDLVMSEVDRCADHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDQSKCTSNELPEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCHARGKQDISQRLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWAGMTRFLLEISNLETISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKGDNSFLQATVAKLGPLPRILGDITRSLSSPTPIQQQLRRFQDHSSAHDISGSVSSGLQRIFEDPADSEMRSIKSPVQEHMEALVRGKHPLLGQQSSAHSMSFSDKEERDSPLPNGRSISLMDLQDSYLAQGHPGPNSLNEASSRLGRVGSQASIGPVPPPHLHQPPVHPKVPQLRDNLPQSAPQVRRPIHPSLSQQRSLQPLSFQNPVYHLSNLHAQSTHSTQSTHSAQSLQPDSSSENLSTGSSRSASPSASGGRGATPRARIPSTSSVEEEFSRRSIQGQETPPPTARWHPPLADQHSGAQVVAVPRQSTTGTAHIVKVEQQSRGVGLVAANAGARTPRSLPHSTSIRSGSSANTEPIQQSGERSRQQSTCSKDGSVPGGRANKQVQSPVESVTMSPVERTAAWVLNNGQYEDEEEEGQSKDDAKHVEKYEQEISKLKERLRTSSRRLEEYERRLLAQEQQMQKLLLEYKTRLEDSEDRLRRQQEEKDSQMKSIICRLMAVEEELKRDHAEMQAVIDAKQKIIDAQEKRISSLDAANSRLMSALTQVKERYSMHNLRNGLSPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_005171321.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171321.3

ORF Insert Sequence:

ATGAGTTTAA CACGATTGGC CACACTGGAG GAACAAACGG GATCGAACAG GAGCCCATCG 
CGCTCTTCTT TTGGCAAGGA TAGTGTTCGT CGCTGGCGTC TTCTCAACAG GCCTTGGCTG 
AAGCACCGTT CAAATTCAGC TCCCGAGGAG CCCTTGGACA TGCATGACAG AAGGATCCTG 
GTTAGACAGG ACATGCGCAC ACAAAGCTTG ACATGGTCCA GAAGCAGCAG TACTAGGCCA 
TCCAATCCCC CTATCTCTAG GGTCCACAGT CTACCAGACC CTCAGCGGGT CACCAGCCGC 
GGTGTCCTCT TGGAGCATGC TAATCTTCAG AAGGACTGGT TATCTCGCAG CTCTTCGCCT 
TTTTTTAAGT CCACATCCGA ACTGGGGAGC GAAATTCACA CTGGGGCTCC ACCGCTCAGA 
CGGGTGTCAA GCTCCCATAT GTTTTCAGGG ATTCTGCTGT CCATGTCAAG ACGAATTAGC 
CGGATTTTAA ATACCTCTCC TGAGCCAGCA GTGGGAGAAC TTTCAGAAGA CGATGTGAAG 
GGTCCCGCCA GTCACCACTT CTCCTGCGGA CAGTCGCCTT TCACCGAGCC CTGCGTTTGG 
GAGAGGAAAT ACTGCATCCT GACTGATAGC CAGCTCATCC TGCTCAACAA GGAGGAAGAG 
ATGCCGTCAG AGGTGCATGA AAGCCCCTCA GCATCCTCTT CGAAGGGCCG CAGTTTGAGG 
CGGACAGTCA GTGTGCCTTC TGAAGGACCG TTCCCAGACT TCCCACCAGA GGGAGCCTCC 
ATGATAGCGG AAGCGTCCGC GGAGCGCTCT CCCAGACGGA GGAGCATATC AGGGCTAGGC 
AGCTCAGAGA AGAACATTTC CATCGATGGC CCCAACTCAT CTCCCTTCAA AGTGCCTGGT 
TTTTTAAGTA AACGACTGAA AGGATCCATC AAAAGGACGA AGAGCCAGAC TAAGCTGGAC 
CGCAACACAA GTTTCAGACT CCCATCGCTG CGACCAACAG AAAATGACAG GTCACGAGGC 
CTGCCCAAAT TGAAGGAGTC CTGTTCCCAT GAGTCTTTGC TGAGTCCTGG CAGCGCTGTT 
GAAGCTCTGG ATCTGAGTAT GGAGGAGGAT GTCTACATCA AACCTTTACA CAGCAGCATC 
CTAGGACAAG ATTTCTGCTT TGAGGTGGCG TACTCAGGTG GAAGTAAGTG CTTCAGCTGT 
TCCTCTGCTG CTGAACGGGA CAAATGGATG GAGAACCTCA AAAGAACTGT GCAACCTAAT 
AAGGATAATT GCCGGCGGGC GGAAAACGTC CTCCGCCTGT GGATCATTGA GGCAAAAGAC 
CTGCCACCAA AAAAGAAATA TTTCTGTGAG CTGTGTCTGG ATGACATGCT GTACGCTCGT 
ACCACCAGCA AAACCCGCTC CGACTGCCTG TTCTGGGGGG AACACTTTGA GTTTTCAGGC 
CTCCCGTCCA TGAAAAGCAT CACCATACAC ATCTACCGGG ATGTGGACAA GAAAAAGAAA 
AAGGACAAAA ACAACTATGT TGGCCTGGTG AACATCCCTG TGCATGGGGT GATGGGTAGG 
CAGTTTGTGG AGAAGTGGTA TCCAGTCAGC ACTCCCACCA CCAGCAAAGC CAAAGGTGGA 
GGCCCCTCCA TCCGCATCAA ATCCCGCTTT CAGACCATCT CAATTCTGCC CATGGAGCAG 
TACAAGGAGT TTGCGGAGTT TGTTACCAAT AACTATACCA TGTTGTGCTC TGTGTTGGAG 
CCGGTCATAA GCGTGAAGAA CAAAGAGGAG ATGGCCAGCG CTCTGGTGCA CATTCTGCAG 
AGCACGGGAC GGGCAAAGGA CTTCCTAACG GATTTGGTGA TGTCAGAAGT GGATCGCTGT 
GCTGACCATG ATGTGCTGAT CTTTAGAGAA AACACACTAG CCACCAAAGC CATTGAAGAA 
TACCTCAAAC TGGTGGGACA GAAGTACCTA CATGATGCAC TAGGGGAGTT TATTAAAGCC 
CTGTACGAGT CGGATGAAAA TTGTGAAGTA GACCAATCAA AGTGCACCAG CAACGAGCTT 
CCAGAGCATC AGAGTAACCT GAAGATGTGC TGCGAACTGG CCTTCTGCAA GATCATCAAC 
TCTTATTGTG TTTTTCCACG TGAACTGAAG GAGGTCTTTG CATCATGGAA GCAGCAGTGT 
CACGCTCGAG GGAAGCAGGA CATCAGTCAG CGTCTTATCA GCGCATCGCT CTTCCTGCGC 
TTTCTGTGTC CTGCCATCAT GTCCCCGTCA CTCTTTGGTC TCATGCAGGA ATATCCAGAT 
GACCGTACTT CTCGAACACT CACCCTCATT GCCAAAGTCA TTCAGAATCT TGCTAACTTT 
GCCAAATTCG GGAACAAAGA GGAGTACATG GCTTTCATGA ATGACTTTCT AGAGCACGAG 
TGGGCTGGTA TGACCCGTTT TCTGCTTGAG ATCTCGAATC TTGAGACAAT CTCCAACACC 
CCGGGCTTTG AGGGCTACAT TGACCTGGGC CGGGAGCTGT CAGTGCTTCA CTCGCTGCTC 
TGGGAGGTGG TGTCTCAGCT GGACAAGGGT GACAATTCCT TCCTGCAGGC GACGGTGGCC 
AAACTGGGGC CCCTGCCACG TATCCTTGGC GATATCACCC GCTCGCTGTC CAGCCCTACT 
CCCATCCAGC AGCAGCTCAG GCGCTTCCAG GACCACAGCT CTGCTCATGA CATCAGTGGA 
AGTGTGTCCT CAGGACTGCA GAGAATATTT GAAGACCCAG CAGACAGTGA AATGAGGAGC 
ATCAAATCAC CTGTCCAAGA GCACATGGAG GCTTTAGTCA GAGGAAAGCA TCCTTTACTG 
GGTCAACAGT CGTCCGCACA CAGCATGAGC TTCTCCGACA AAGAGGAAAG GGACAGTCCG 
CTCCCGAATG GACGCAGTAT ATCTTTAATG GACTTGCAGG ACTCTTACCT TGCTCAGGGG 
CATCCAGGTC CCAACTCGCT CAATGAAGCC TCATCTAGGT TAGGCAGAGT AGGCTCTCAG 
GCCTCCATCG GCCCTGTCCC TCCTCCACAC CTCCACCAGC CTCCAGTCCA CCCGAAAGTT 
CCACAGCTGA GGGATAACTT ACCCCAGAGT GCACCACAGG TGCGGCGCCC GATCCACCCA 
TCCCTTAGCC AGCAGCGCAG CCTACAACCG CTGTCTTTCC AAAACCCTGT TTACCACCTC 
AGCAACCTGC ACGCACAATC AACACACTCA ACCCAGTCCA CACACTCTGC CCAGTCCCTG 
CAGCCTGACT CTAGCTCAGA AAACCTGAGC ACAGGGAGCT CTCGGAGCGC CAGTCCTAGT 
GCCTCAGGTG GCCGGGGAGC AACTCCCAGA GCCCGGATAC CCTCCACAAG CAGCGTGGAG 
GAGGAATTTA GCCGAAGAAG CATCCAGGGA CAGGAGACGC CGCCTCCAAC GGCACGCTGG 
CACCCTCCCC TGGCAGACCA GCACTCGGGA GCCCAGGTGG TGGCAGTGCC AAGGCAGAGT 
ACCACCGGTA CGGCACATAT AGTGAAAGTG GAGCAGCAGA GTCGAGGAGT TGGGTTGGTG 
GCAGCAAATG CTGGAGCACG AACCCCAAGG TCGCTCCCTC ACAGCACGTC CATCCGCAGC 
GGCAGCAGCG CCAACACTGA ACCCATACAG CAGAGCGGTG AACGATCAAG ACAGCAGTCC 
ACGTGCTCCA AAGATGGTTC AGTACCAGGA GGACGAGCCA ACAAACAGGT CCAGTCACCT 
GTAGAGTCTG TCACCATGTC CCCAGTGGAG CGAACAGCTG CGTGGGTTTT GAATAATGGC 
CAGTATGAAG ATGAGGAAGA GGAAGGGCAG AGTAAAGACG ATGCCAAACA TGTGGAGAAG 
TATGAACAGG AAATCTCGAA GCTAAAGGAG CGTCTGCGGA CGTCTAGTCG GCGGCTGGAG 
GAGTATGAGA GACGGTTACT GGCTCAGGAG CAGCAGATGC AGAAGCTGCT CTTAGAATAC 
AAGACTCGAC TGGAGGACAG TGAGGACCGT CTGCGAAGAC AACAGGAGGA AAAAGACAGC 
CAGATGAAGA GTATTATCTG CAGGCTGATG GCCGTGGAGG AAGAGCTGAA GAGAGACCAT 
GCAGAGATGC AGGCAGTCAT AGATGCCAAA CAGAAGATAA TCGATGCACA GGAGAAGAGG 
ATCAGCTCTC TGGATGCTGC TAACTCTCGG CTGATGAGCG CTCTGACTCA GGTGAAGGAG 
CGCTACAGCA TGCACAACCT CCGGAACGGC CTGTCACCCA CCAACCCCAC CAAACTCTCC 
ATCACAGAGA ACGGGGAGTT CAAGAATAGC AGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasal2 ( XM_005171321.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171378.1 Danio rerio RAS protein activator like 2 (rasal2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa07236
Clone ID Related Accession (Same CDS sequence)	XM_005171321.3 , XM_005171321.4
Accession Version	XM_005171321.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras GTPase-activating protein nGAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171321.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTTAA CACGATTGGC CACACTGGAG GAACAAACGG GATCGAACAG GAGCCCATCG 
CGCTCTTCTT TTGGCAAGGA TAGTGTTCGT CGCTGGCGTC TTCTCAACAG GCCTTGGCTG 
AAGCACCGTT CAAATTCAGC TCCCGAGGAG CCCTTGGACA TGCATGACAG AAGGATCCTG 
GTTAGACAGG ACATGCGCAC ACAAAGCTTG ACATGGTCCA GAAGCAGCAG TACTAGGCCA 
TCCAATCCCC CTATCTCTAG GGTCCACAGT CTACCAGACC CTCAGCGGGT CACCAGCCGC 
GGTGTCCTCT TGGAGCATGC TAATCTTCAG AAGGACTGGT TATCTCGCAG CTCTTCGCCT 
TTTTTTAAGT CCACATCCGA ACTGGGGAGC GAAATTCACA CTGGGGCTCC ACCGCTCAGA 
CGGGTGTCAA GCTCCCATAT GTTTTCAGGG ATTCTGCTGT CCATGTCAAG ACGAATTAGC 
CGGATTTTAA ATACCTCTCC TGAGCCAGCA GTGGGAGAAC TTTCAGAAGA CGATGTGAAG 
GGTCCCGCCA GTCACCACTT CTCCTGCGGA CAGTCGCCTT TCACCGAGCC CTGCGTTTGG 
GAGAGGAAAT ACTGCATCCT GACTGATAGC CAGCTCATCC TGCTCAACAA GGAGGAAGAG 
ATGCCGTCAG AGGTGCATGA AAGCCCCTCA GCATCCTCTT CGAAGGGCCG CAGTTTGAGG 
CGGACAGTCA GTGTGCCTTC TGAAGGACCG TTCCCAGACT TCCCACCAGA GGGAGCCTCC 
ATGATAGCGG AAGCGTCCGC GGAGCGCTCT CCCAGACGGA GGAGCATATC AGGGCTAGGC 
AGCTCAGAGA AGAACATTTC CATCGATGGC CCCAACTCAT CTCCCTTCAA AGTGCCTGGT 
TTTTTAAGTA AACGACTGAA AGGATCCATC AAAAGGACGA AGAGCCAGAC TAAGCTGGAC 
CGCAACACAA GTTTCAGACT CCCATCGCTG CGACCAACAG AAAATGACAG GTCACGAGGC 
CTGCCCAAAT TGAAGGAGTC CTGTTCCCAT GAGTCTTTGC TGAGTCCTGG CAGCGCTGTT 
GAAGCTCTGG ATCTGAGTAT GGAGGAGGAT GTCTACATCA AACCTTTACA CAGCAGCATC 
CTAGGACAAG ATTTCTGCTT TGAGGTGGCG TACTCAGGTG GAAGTAAGTG CTTCAGCTGT 
TCCTCTGCTG CTGAACGGGA CAAATGGATG GAGAACCTCA AAAGAACTGT GCAACCTAAT 
AAGGATAATT GCCGGCGGGC GGAAAACGTC CTCCGCCTGT GGATCATTGA GGCAAAAGAC 
CTGCCACCAA AAAAGAAATA TTTCTGTGAG CTGTGTCTGG ATGACATGCT GTACGCTCGT 
ACCACCAGCA AAACCCGCTC CGACTGCCTG TTCTGGGGGG AACACTTTGA GTTTTCAGGC 
CTCCCGTCCA TGAAAAGCAT CACCATACAC ATCTACCGGG ATGTGGACAA GAAAAAGAAA 
AAGGACAAAA ACAACTATGT TGGCCTGGTG AACATCCCTG TGCATGGGGT GATGGGTAGG 
CAGTTTGTGG AGAAGTGGTA TCCAGTCAGC ACTCCCACCA CCAGCAAAGC CAAAGGTGGA 
GGCCCCTCCA TCCGCATCAA ATCCCGCTTT CAGACCATCT CAATTCTGCC CATGGAGCAG 
TACAAGGAGT TTGCGGAGTT TGTTACCAAT AACTATACCA TGTTGTGCTC TGTGTTGGAG 
CCGGTCATAA GCGTGAAGAA CAAAGAGGAG ATGGCCAGCG CTCTGGTGCA CATTCTGCAG 
AGCACGGGAC GGGCAAAGGA CTTCCTAACG GATTTGGTGA TGTCAGAAGT GGATCGCTGT 
GCTGACCATG ATGTGCTGAT CTTTAGAGAA AACACACTAG CCACCAAAGC CATTGAAGAA 
TACCTCAAAC TGGTGGGACA GAAGTACCTA CATGATGCAC TAGGGGAGTT TATTAAAGCC 
CTGTACGAGT CGGATGAAAA TTGTGAAGTA GACCAATCAA AGTGCACCAG CAACGAGCTT 
CCAGAGCATC AGAGTAACCT GAAGATGTGC TGCGAACTGG CCTTCTGCAA GATCATCAAC 
TCTTATTGTG TTTTTCCACG TGAACTGAAG GAGGTCTTTG CATCATGGAA GCAGCAGTGT 
CACGCTCGAG GGAAGCAGGA CATCAGTCAG CGTCTTATCA GCGCATCGCT CTTCCTGCGC 
TTTCTGTGTC CTGCCATCAT GTCCCCGTCA CTCTTTGGTC TCATGCAGGA ATATCCAGAT 
GACCGTACTT CTCGAACACT CACCCTCATT GCCAAAGTCA TTCAGAATCT TGCTAACTTT 
GCCAAATTCG GGAACAAAGA GGAGTACATG GCTTTCATGA ATGACTTTCT AGAGCACGAG 
TGGGCTGGTA TGACCCGTTT TCTGCTTGAG ATCTCGAATC TTGAGACAAT CTCCAACACC 
CCGGGCTTTG AGGGCTACAT TGACCTGGGC CGGGAGCTGT CAGTGCTTCA CTCGCTGCTC 
TGGGAGGTGG TGTCTCAGCT GGACAAGGGT GACAATTCCT TCCTGCAGGC GACGGTGGCC 
AAACTGGGGC CCCTGCCACG TATCCTTGGC GATATCACCC GCTCGCTGTC CAGCCCTACT 
CCCATCCAGC AGCAGCTCAG GCGCTTCCAG GACCACAGCT CTGCTCATGA CATCAGTGGA 
AGTGTGTCCT CAGGACTGCA GAGAATATTT GAAGACCCAG CAGACAGTGA AATGAGGAGC 
ATCAAATCAC CTGTCCAAGA GCACATGGAG GCTTTAGTCA GAGGAAAGCA TCCTTTACTG 
GGTCAACAGT CGTCCGCACA CAGCATGAGC TTCTCCGACA AAGAGGAAAG GGACAGTCCG 
CTCCCGAATG GACGCAGTAT ATCTTTAATG GACTTGCAGG ACTCTTACCT TGCTCAGGGG 
CATCCAGGTC CCAACTCGCT CAATGAAGCC TCATCTAGGT TAGGCAGAGT AGGCTCTCAG 
GCCTCCATCG GCCCTGTCCC TCCTCCACAC CTCCACCAGC CTCCAGTCCA CCCGAAAGTT 
CCACAGCTGA GGGATAACTT ACCCCAGAGT GCACCACAGG TGCGGCGCCC GATCCACCCA 
TCCCTTAGCC AGCAGCGCAG CCTACAACCG CTGTCTTTCC AAAACCCTGT TTACCACCTC 
AGCAACCTGC ACGCACAATC AACACACTCA ACCCAGTCCA CACACTCTGC CCAGTCCCTG 
CAGCCTGACT CTAGCTCAGA AAACCTGAGC ACAGGGAGCT CTCGGAGCGC CAGTCCTAGT 
GCCTCAGGTG GCCGGGGAGC AACTCCCAGA GCCCGGATAC CCTCCACAAG CAGCGTGGAG 
GAGGAATTTA GCCGAAGAAG CATCCAGGGA CAGGAGACGC CGCCTCCAAC GGCACGCTGG 
CACCCTCCCC TGGCAGACCA GCACTCGGGA GCCCAGGTGG TGGCAGTGCC AAGGCAGAGT 
ACCACCGGTA CGGCACATAT AGTGAAAGTG GAGCAGCAGA GTCGAGGAGT TGGGTTGGTG 
GCAGCAAATG CTGGAGCACG AACCCCAAGG TCGCTCCCTC ACAGCACGTC CATCCGCAGC 
GGCAGCAGCG CCAACACTGA ACCCATACAG CAGAGCGGTG AACGATCAAG ACAGCAGTCC 
ACGTGCTCCA AAGATGGTTC AGTACCAGGA GGACGAGCCA ACAAACAGGT CCAGTCACCT 
GTAGAGTCTG TCACCATGTC CCCAGTGGAG CGAACAGCTG CGTGGGTTTT GAATAATGGC 
CAGTATGAAG ATGAGGAAGA GGAAGGGCAG AGTAAAGACG ATGCCAAACA TGTGGAGAAG 
TATGAACAGG AAATCTCGAA GCTAAAGGAG CGTCTGCGGA CGTCTAGTCG GCGGCTGGAG 
GAGTATGAGA GACGGTTACT GGCTCAGGAG CAGCAGATGC AGAAGCTGCT CTTAGAATAC 
AAGACTCGAC TGGAGGACAG TGAGGACCGT CTGCGAAGAC AACAGGAGGA AAAAGACAGC 
CAGATGAAGA GTATTATCTG CAGGCTGATG GCCGTGGAGG AAGAGCTGAA GAGAGACCAT 
GCAGAGATGC AGGCAGTCAT AGATGCCAAA CAGAAGATAA TCGATGCACA GGAGAAGAGG 
ATCAGCTCTC TGGATGCTGC TAACTCTCGG CTGATGAGCG CTCTGACTCA GGTGAAGGAG 
CGCTACAGCA TGCACAACCT CCGGAACGGC CTGTCACCCA CCAACCCCAC CAAACTCTCC 
ATCACAGAGA ACGGGGAGTT CAAGAATAGC AGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171378.1
CDS	177..4475
Translation	MSLTRLATLEEQTGSNRSPSRSSFGKDSVRRWRLLNRPWLKHRSNSAPEEPLDMHDRRILVRQDMRTQSLTWSRSSSTRPSNPPISRVHSLPDPQRVTSRGVLLEHANLQKDWLSRSSSPFFKSTSELGSEIHTGAPPLRRVSSSHMFSGILLSMSRRISRILNTSPEPAVGELSEDDVKGPASHHFSCGQSPFTEPCVWERKYCILTDSQLILLNKEEEMPSEVHESPSASSSKGRSLRRTVSVPSEGPFPDFPPEGASMIAEASAERSPRRRSISGLGSSEKNISIDGPNSSPFKVPGFLSKRLKGSIKRTKSQTKLDRNTSFRLPSLRPTENDRSRGLPKLKESCSHESLLSPGSAVEALDLSMEEDVYIKPLHSSILGQDFCFEVAYSGGSKCFSCSSAAERDKWMENLKRTVQPNKDNCRRAENVLRLWIIEAKDLPPKKKYFCELCLDDMLYARTTSKTRSDCLFWGEHFEFSGLPSMKSITIHIYRDVDKKKKKDKNNYVGLVNIPVHGVMGRQFVEKWYPVSTPTTSKAKGGGPSIRIKSRFQTISILPMEQYKEFAEFVTNNYTMLCSVLEPVISVKNKEEMASALVHILQSTGRAKDFLTDLVMSEVDRCADHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDQSKCTSNELPEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCHARGKQDISQRLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWAGMTRFLLEISNLETISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKGDNSFLQATVAKLGPLPRILGDITRSLSSPTPIQQQLRRFQDHSSAHDISGSVSSGLQRIFEDPADSEMRSIKSPVQEHMEALVRGKHPLLGQQSSAHSMSFSDKEERDSPLPNGRSISLMDLQDSYLAQGHPGPNSLNEASSRLGRVGSQASIGPVPPPHLHQPPVHPKVPQLRDNLPQSAPQVRRPIHPSLSQQRSLQPLSFQNPVYHLSNLHAQSTHSTQSTHSAQSLQPDSSSENLSTGSSRSASPSASGGRGATPRARIPSTSSVEEEFSRRSIQGQETPPPTARWHPPLADQHSGAQVVAVPRQSTTGTAHIVKVEQQSRGVGLVAANAGARTPRSLPHSTSIRSGSSANTEPIQQSGERSRQQSTCSKDGSVPGGRANKQVQSPVESVTMSPVERTAAWVLNNGQYEDEEEEGQSKDDAKHVEKYEQEISKLKERLRTSSRRLEEYERRLLAQEQQMQKLLLEYKTRLEDSEDRLRRQQEEKDSQMKSIICRLMAVEEELKRDHAEMQAVIDAKQKIIDAQEKRISSLDAANSRLMSALTQVKERYSMHNLRNGLSPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_005171321.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171321.4

ORF Insert Sequence:

ATGAGTTTAA CACGATTGGC CACACTGGAG GAACAAACGG GATCGAACAG GAGCCCATCG 
CGCTCTTCTT TTGGCAAGGA TAGTGTTCGT CGCTGGCGTC TTCTCAACAG GCCTTGGCTG 
AAGCACCGTT CAAATTCAGC TCCCGAGGAG CCCTTGGACA TGCATGACAG AAGGATCCTG 
GTTAGACAGG ACATGCGCAC ACAAAGCTTG ACATGGTCCA GAAGCAGCAG TACTAGGCCA 
TCCAATCCCC CTATCTCTAG GGTCCACAGT CTACCAGACC CTCAGCGGGT CACCAGCCGC 
GGTGTCCTCT TGGAGCATGC TAATCTTCAG AAGGACTGGT TATCTCGCAG CTCTTCGCCT 
TTTTTTAAGT CCACATCCGA ACTGGGGAGC GAAATTCACA CTGGGGCTCC ACCGCTCAGA 
CGGGTGTCAA GCTCCCATAT GTTTTCAGGG ATTCTGCTGT CCATGTCAAG ACGAATTAGC 
CGGATTTTAA ATACCTCTCC TGAGCCAGCA GTGGGAGAAC TTTCAGAAGA CGATGTGAAG 
GGTCCCGCCA GTCACCACTT CTCCTGCGGA CAGTCGCCTT TCACCGAGCC CTGCGTTTGG 
GAGAGGAAAT ACTGCATCCT GACTGATAGC CAGCTCATCC TGCTCAACAA GGAGGAAGAG 
ATGCCGTCAG AGGTGCATGA AAGCCCCTCA GCATCCTCTT CGAAGGGCCG CAGTTTGAGG 
CGGACAGTCA GTGTGCCTTC TGAAGGACCG TTCCCAGACT TCCCACCAGA GGGAGCCTCC 
ATGATAGCGG AAGCGTCCGC GGAGCGCTCT CCCAGACGGA GGAGCATATC AGGGCTAGGC 
AGCTCAGAGA AGAACATTTC CATCGATGGC CCCAACTCAT CTCCCTTCAA AGTGCCTGGT 
TTTTTAAGTA AACGACTGAA AGGATCCATC AAAAGGACGA AGAGCCAGAC TAAGCTGGAC 
CGCAACACAA GTTTCAGACT CCCATCGCTG CGACCAACAG AAAATGACAG GTCACGAGGC 
CTGCCCAAAT TGAAGGAGTC CTGTTCCCAT GAGTCTTTGC TGAGTCCTGG CAGCGCTGTT 
GAAGCTCTGG ATCTGAGTAT GGAGGAGGAT GTCTACATCA AACCTTTACA CAGCAGCATC 
CTAGGACAAG ATTTCTGCTT TGAGGTGGCG TACTCAGGTG GAAGTAAGTG CTTCAGCTGT 
TCCTCTGCTG CTGAACGGGA CAAATGGATG GAGAACCTCA AAAGAACTGT GCAACCTAAT 
AAGGATAATT GCCGGCGGGC GGAAAACGTC CTCCGCCTGT GGATCATTGA GGCAAAAGAC 
CTGCCACCAA AAAAGAAATA TTTCTGTGAG CTGTGTCTGG ATGACATGCT GTACGCTCGT 
ACCACCAGCA AAACCCGCTC CGACTGCCTG TTCTGGGGGG AACACTTTGA GTTTTCAGGC 
CTCCCGTCCA TGAAAAGCAT CACCATACAC ATCTACCGGG ATGTGGACAA GAAAAAGAAA 
AAGGACAAAA ACAACTATGT TGGCCTGGTG AACATCCCTG TGCATGGGGT GATGGGTAGG 
CAGTTTGTGG AGAAGTGGTA TCCAGTCAGC ACTCCCACCA CCAGCAAAGC CAAAGGTGGA 
GGCCCCTCCA TCCGCATCAA ATCCCGCTTT CAGACCATCT CAATTCTGCC CATGGAGCAG 
TACAAGGAGT TTGCGGAGTT TGTTACCAAT AACTATACCA TGTTGTGCTC TGTGTTGGAG 
CCGGTCATAA GCGTGAAGAA CAAAGAGGAG ATGGCCAGCG CTCTGGTGCA CATTCTGCAG 
AGCACGGGAC GGGCAAAGGA CTTCCTAACG GATTTGGTGA TGTCAGAAGT GGATCGCTGT 
GCTGACCATG ATGTGCTGAT CTTTAGAGAA AACACACTAG CCACCAAAGC CATTGAAGAA 
TACCTCAAAC TGGTGGGACA GAAGTACCTA CATGATGCAC TAGGGGAGTT TATTAAAGCC 
CTGTACGAGT CGGATGAAAA TTGTGAAGTA GACCAATCAA AGTGCACCAG CAACGAGCTT 
CCAGAGCATC AGAGTAACCT GAAGATGTGC TGCGAACTGG CCTTCTGCAA GATCATCAAC 
TCTTATTGTG TTTTTCCACG TGAACTGAAG GAGGTCTTTG CATCATGGAA GCAGCAGTGT 
CACGCTCGAG GGAAGCAGGA CATCAGTCAG CGTCTTATCA GCGCATCGCT CTTCCTGCGC 
TTTCTGTGTC CTGCCATCAT GTCCCCGTCA CTCTTTGGTC TCATGCAGGA ATATCCAGAT 
GACCGTACTT CTCGAACACT CACCCTCATT GCCAAAGTCA TTCAGAATCT TGCTAACTTT 
GCCAAATTCG GGAACAAAGA GGAGTACATG GCTTTCATGA ATGACTTTCT AGAGCACGAG 
TGGGCTGGTA TGACCCGTTT TCTGCTTGAG ATCTCGAATC TTGAGACAAT CTCCAACACC 
CCGGGCTTTG AGGGCTACAT TGACCTGGGC CGGGAGCTGT CAGTGCTTCA CTCGCTGCTC 
TGGGAGGTGG TGTCTCAGCT GGACAAGGGT GACAATTCCT TCCTGCAGGC GACGGTGGCC 
AAACTGGGGC CCCTGCCACG TATCCTTGGC GATATCACCC GCTCGCTGTC CAGCCCTACT 
CCCATCCAGC AGCAGCTCAG GCGCTTCCAG GACCACAGCT CTGCTCATGA CATCAGTGGA 
AGTGTGTCCT CAGGACTGCA GAGAATATTT GAAGACCCAG CAGACAGTGA AATGAGGAGC 
ATCAAATCAC CTGTCCAAGA GCACATGGAG GCTTTAGTCA GAGGAAAGCA TCCTTTACTG 
GGTCAACAGT CGTCCGCACA CAGCATGAGC TTCTCCGACA AAGAGGAAAG GGACAGTCCG 
CTCCCGAATG GACGCAGTAT ATCTTTAATG GACTTGCAGG ACTCTTACCT TGCTCAGGGG 
CATCCAGGTC CCAACTCGCT CAATGAAGCC TCATCTAGGT TAGGCAGAGT AGGCTCTCAG 
GCCTCCATCG GCCCTGTCCC TCCTCCACAC CTCCACCAGC CTCCAGTCCA CCCGAAAGTT 
CCACAGCTGA GGGATAACTT ACCCCAGAGT GCACCACAGG TGCGGCGCCC GATCCACCCA 
TCCCTTAGCC AGCAGCGCAG CCTACAACCG CTGTCTTTCC AAAACCCTGT TTACCACCTC 
AGCAACCTGC ACGCACAATC AACACACTCA ACCCAGTCCA CACACTCTGC CCAGTCCCTG 
CAGCCTGACT CTAGCTCAGA AAACCTGAGC ACAGGGAGCT CTCGGAGCGC CAGTCCTAGT 
GCCTCAGGTG GCCGGGGAGC AACTCCCAGA GCCCGGATAC CCTCCACAAG CAGCGTGGAG 
GAGGAATTTA GCCGAAGAAG CATCCAGGGA CAGGAGACGC CGCCTCCAAC GGCACGCTGG 
CACCCTCCCC TGGCAGACCA GCACTCGGGA GCCCAGGTGG TGGCAGTGCC AAGGCAGAGT 
ACCACCGGTA CGGCACATAT AGTGAAAGTG GAGCAGCAGA GTCGAGGAGT TGGGTTGGTG 
GCAGCAAATG CTGGAGCACG AACCCCAAGG TCGCTCCCTC ACAGCACGTC CATCCGCAGC 
GGCAGCAGCG CCAACACTGA ACCCATACAG CAGAGCGGTG AACGATCAAG ACAGCAGTCC 
ACGTGCTCCA AAGATGGTTC AGTACCAGGA GGACGAGCCA ACAAACAGGT CCAGTCACCT 
GTAGAGTCTG TCACCATGTC CCCAGTGGAG CGAACAGCTG CGTGGGTTTT GAATAATGGC 
CAGTATGAAG ATGAGGAAGA GGAAGGGCAG AGTAAAGACG ATGCCAAACA TGTGGAGAAG 
TATGAACAGG AAATCTCGAA GCTAAAGGAG CGTCTGCGGA CGTCTAGTCG GCGGCTGGAG 
GAGTATGAGA GACGGTTACT GGCTCAGGAG CAGCAGATGC AGAAGCTGCT CTTAGAATAC 
AAGACTCGAC TGGAGGACAG TGAGGACCGT CTGCGAAGAC AACAGGAGGA AAAAGACAGC 
CAGATGAAGA GTATTATCTG CAGGCTGATG GCCGTGGAGG AAGAGCTGAA GAGAGACCAT 
GCAGAGATGC AGGCAGTCAT AGATGCCAAA CAGAAGATAA TCGATGCACA GGAGAAGAGG 
ATCAGCTCTC TGGATGCTGC TAACTCTCGG CTGATGAGCG CTCTGACTCA GGTGAAGGAG 
CGCTACAGCA TGCACAACCT CCGGAACGGC CTGTCACCCA CCAACCCCAC CAAACTCTCC 
ATCACAGAGA ACGGGGAGTT CAAGAATAGC AGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasal2 ( XM_005171322.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171379.1 Danio rerio RAS protein activator like 2 (rasal2), transcript variant X2, mRNA.

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CloneID	ODa07237
Clone ID Related Accession (Same CDS sequence)	XM_005171322.4 , XM_005171322.3
Accession Version	XM_005171322.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras GTPase-activating protein nGAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171322.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTTAA CACGATTGGC CACACTGGAG GAACAAACGG GATCGAACAG GAGCCCATCG 
CGCTCTTCTT TTGGCAAGGA TAGTGTTCGT CGCTGGCGTC TTCTCAACAG GCCTTGGCTG 
AAGCACCGTT CAAATTCAGC TCCCGAGGAG CCCTTGGACA TGCATGACAG AAGGATCCTG 
GTTAGACAGG ACATGCGCAC ACAAAGCTTG ACATGGTCCA GAAGCAGCAG TACTAGGCCA 
TCCAATCCCC CTATCTCTAG GGTCCACAGT CTACCAGACC CTCAGCGGGT CACCAGCCGC 
GGTGTCCTCT TGGAGCATGC TAATCTTCAG AAGGACTGGT TATCTCGCAG CTCTTCGCCT 
TTTTTTAAGT CCACATCCGA ACTGGGGAGC GAAATTCACA CTGGGGCTCC ACCGCTCAGA 
CGGGTGTCAA GCTCCCATAT GTTTTCAGGG ATTCTGCTGT CCATGTCAAG ACGAATTAGC 
CGGATTTTAA ATACCTCTCC TGAGCCAGCA GTGGGAGAAC TTTCAGAAGA CGATGTGAAG 
GGTCCCGCCA GTCACCACTT CTCCTGCGGA CAGTCGCCTT TCACCGAGCC CTGCGTTTGG 
GAGAGGAAAT ACTGCATCCT GACTGATAGC CAGCTCATCC TGCTCAACAA GGAGGAAGAG 
ATGCCGTCAG AGGTGCATGA AAGCCCCTCA GCATCCTCTT CGAAGGGCCG CAGTTTGAGG 
CGGACAGTCA GTGTGCCTTC TGAAGGACCG TTCCCAGACT TCCCACCAGA GGGAGCCTCC 
ATGATAGCGG AAGCGTCCGC GGAGCGCTCT CCCAGACGGA GGAGCATATC AGGGCTAGGC 
AGCTCAGAGA AGAACATTTC CATCGATGGC CCCAACTCAT CTCCCTTCAA AGTGCCTGGT 
TTTTTAAGTA AACGACTGAA AGGATCCATC AAAAGGACGA AGAGCCAGAC TAAGCTGGAC 
CGCAACACAA GTTTCAGACT CCCATCGCTG CGACCAACAG AAAATGACAG GTCACGAGGC 
CTGCCCAAAT TGAAGGAGTC CTGTTCCCAT GAGTCTTTGC TGAGTCCTGG CAGCGCTGTT 
GAAGCTCTGG ATCTGAGTAT GGAGGAGGAT GTCTACATCA AACCTTTACA CAGCAGCATC 
CTAGGACAAG ATTTCTGCTT TGAGGTGGCG TACTCAGGTG GAAGTAAGTG CTTCAGCTGT 
TCCTCTGCTG CTGAACGGGA CAAATGGATG GAGAACCTCA AAAGAACTGT GCAACCTAAT 
AAGGATAATT GCCGGCGGGC GGAAAACGTC CTCCGCCTGT GGATCATTGA GGCAAAAGAC 
CTGCCACCAA AAAAGAAATA TTTCTGTGAG CTGTGTCTGG ATGACATGCT GTACGCTCGT 
ACCACCAGCA AAACCCGCTC CGACTGCCTG TTCTGGGGGG AACACTTTGA GTTTTCAGGC 
CTCCCGTCCA TGAAAAGCAT CACCATACAC ATCTACCGGG ATGTGGACAA GAAAAAGAAA 
AAGGACAAAA ACAACTATGT TGGCCTGGTG AACATCCCTG TGCATGGGGT GATGGGTAGG 
CAGTTTGTGG AGAAGTGGTA TCCAGTCAGC ACTCCCACCA CCAGCAAAGC CAAAGGTGGA 
GGCCCCTCCA TCCGCATCAA ATCCCGCTTT CAGACCATCT CAATTCTGCC CATGGAGCAG 
TACAAGGAGT TTGCGGAGTT TGTTACCAAT AACTATACCA TGTTGTGCTC TGTGTTGGAG 
CCGGTCATAA GCGTGAAGAA CAAAGAGGAG ATGGCCAGCG CTCTGGTGCA CATTCTGCAG 
AGCACGGGAC GGGCAAAGGA CTTCCTAACG GATTTGGTGA TGTCAGAAGT GGATCGCTGT 
GCTGACCATG ATGTGCTGAT CTTTAGAGAA AACACACTAG CCACCAAAGC CATTGAAGAA 
TACCTCAAAC TGGTGGGACA GAAGTACCTA CATGATGCAC TAGGGGAGTT TATTAAAGCC 
CTGTACGAGT CGGATGAAAA TTGTGAAGTA GACCAATCAA AGTGCACCAG CAACGAGCTT 
CCAGAGCATC AGAGTAACCT GAAGATGTGC TGCGAACTGG CCTTCTGCAA GATCATCAAC 
TCTTATTGTG TTTTTCCACG TGAACTGAAG GAGGTCTTTG CATCATGGAA GCAGCAGTGT 
CACGCTCGAG GGAAGCAGGA CATCAGTCAG CGTCTTATCA GCGCATCGCT CTTCCTGCGC 
TTTCTGTGTC CTGCCATCAT GTCCCCGTCA CTCTTTGGTC TCATGCAGGA ATATCCAGAT 
GACCGTACTT CTCGAACACT CACCCTCATT GCCAAAGTCA TTCAGAATCT TGCTAACTTT 
GCCAAATTCG GGAACAAAGA GGAGTACATG GCTTTCATGA ATGACTTTCT AGAGCACGAG 
TGGGCTGGTA TGACCCGTTT TCTGCTTGAG ATCTCGAATC TTGAGACAAT CTCCAACACC 
CCGGGCTTTG AGGGCTACAT TGACCTGGGC CGGGAGCTGT CAGTGCTTCA CTCGCTGCTC 
TGGGAGGTGG TGTCTCAGCT GGACAAGGCG ACGGTGGCCA AACTGGGGCC CCTGCCACGT 
ATCCTTGGCG ATATCACCCG CTCGCTGTCC AGCCCTACTC CCATCCAGCA GCAGCTCAGG 
CGCTTCCAGG ACCACAGCTC TGCTCATGAC ATCAGTGGAA GTGTGTCCTC AGGACTGCAG 
AGAATATTTG AAGACCCAGC AGACAGTGAA ATGAGGAGCA TCAAATCACC TGTCCAAGAG 
CACATGGAGG CTTTAGTCAG AGGAAAGCAT CCTTTACTGG GTCAACAGTC GTCCGCACAC 
AGCATGAGCT TCTCCGACAA AGAGGAAAGG GACAGTCCGC TCCCGAATGG ACGCAGTATA 
TCTTTAATGG ACTTGCAGGA CTCTTACCTT GCTCAGGGGC ATCCAGGTCC CAACTCGCTC 
AATGAAGCCT CATCTAGGTT AGGCAGAGTA GGCTCTCAGG CCTCCATCGG CCCTGTCCCT 
CCTCCACACC TCCACCAGCC TCCAGTCCAC CCGAAAGTTC CACAGCTGAG GGATAACTTA 
CCCCAGAGTG CACCACAGGT GCGGCGCCCG ATCCACCCAT CCCTTAGCCA GCAGCGCAGC 
CTACAACCGC TGTCTTTCCA AAACCCTGTT TACCACCTCA GCAACCTGCA CGCACAATCA 
ACACACTCAA CCCAGTCCAC ACACTCTGCC CAGTCCCTGC AGCCTGACTC TAGCTCAGAA 
AACCTGAGCA CAGGGAGCTC TCGGAGCGCC AGTCCTAGTG CCTCAGGTGG CCGGGGAGCA 
ACTCCCAGAG CCCGGATACC CTCCACAAGC AGCGTGGAGG AGGAATTTAG CCGAAGAAGC 
ATCCAGGGAC AGGAGACGCC GCCTCCAACG GCACGCTGGC ACCCTCCCCT GGCAGACCAG 
CACTCGGGAG CCCAGGTGGT GGCAGTGCCA AGGCAGAGTA CCACCGGTAC GGCACATATA 
GTGAAAGTGG AGCAGCAGAG TCGAGGAGTT GGGTTGGTGG CAGCAAATGC TGGAGCACGA 
ACCCCAAGGT CGCTCCCTCA CAGCACGTCC ATCCGCAGCG GCAGCAGCGC CAACACTGAA 
CCCATACAGC AGAGCGGTGA ACGATCAAGA CAGCAGTCCA CGTGCTCCAA AGATGGTTCA 
GTACCAGGAG GACGAGCCAA CAAACAGGTC CAGTCACCTG TAGAGTCTGT CACCATGTCC 
CCAGTGGAGC GAACAGCTGC GTGGGTTTTG AATAATGGCC AGTATGAAGA TGAGGAAGAG 
GAAGGGCAGA GTAAAGACGA TGCCAAACAT GTGGAGAAGT ATGAACAGGA AATCTCGAAG 
CTAAAGGAGC GTCTGCGGAC GTCTAGTCGG CGGCTGGAGG AGTATGAGAG ACGGTTACTG 
GCTCAGGAGC AGCAGATGCA GAAGCTGCTC TTAGAATACA AGACTCGACT GGAGGACAGT 
GAGGACCGTC TGCGAAGACA ACAGGAGGAA AAAGACAGCC AGATGAAGAG TATTATCTGC 
AGGCTGATGG CCGTGGAGGA AGAGCTGAAG AGAGACCATG CAGAGATGCA GGCAGTCATA 
GATGCCAAAC AGAAGATAAT CGATGCACAG GAGAAGAGGA TCAGCTCTCT GGATGCTGCT 
AACTCTCGGC TGATGAGCGC TCTGACTCAG GTGAAGGAGC GCTACAGCAT GCACAACCTC 
CGGAACGGCC TGTCACCCAC CAACCCCACC AAACTCTCCA TCACAGAGAA CGGGGAGTTC 
AAGAATAGCA GCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171379.1
CDS	177..4454
Translation	MSLTRLATLEEQTGSNRSPSRSSFGKDSVRRWRLLNRPWLKHRSNSAPEEPLDMHDRRILVRQDMRTQSLTWSRSSSTRPSNPPISRVHSLPDPQRVTSRGVLLEHANLQKDWLSRSSSPFFKSTSELGSEIHTGAPPLRRVSSSHMFSGILLSMSRRISRILNTSPEPAVGELSEDDVKGPASHHFSCGQSPFTEPCVWERKYCILTDSQLILLNKEEEMPSEVHESPSASSSKGRSLRRTVSVPSEGPFPDFPPEGASMIAEASAERSPRRRSISGLGSSEKNISIDGPNSSPFKVPGFLSKRLKGSIKRTKSQTKLDRNTSFRLPSLRPTENDRSRGLPKLKESCSHESLLSPGSAVEALDLSMEEDVYIKPLHSSILGQDFCFEVAYSGGSKCFSCSSAAERDKWMENLKRTVQPNKDNCRRAENVLRLWIIEAKDLPPKKKYFCELCLDDMLYARTTSKTRSDCLFWGEHFEFSGLPSMKSITIHIYRDVDKKKKKDKNNYVGLVNIPVHGVMGRQFVEKWYPVSTPTTSKAKGGGPSIRIKSRFQTISILPMEQYKEFAEFVTNNYTMLCSVLEPVISVKNKEEMASALVHILQSTGRAKDFLTDLVMSEVDRCADHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDQSKCTSNELPEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCHARGKQDISQRLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWAGMTRFLLEISNLETISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRILGDITRSLSSPTPIQQQLRRFQDHSSAHDISGSVSSGLQRIFEDPADSEMRSIKSPVQEHMEALVRGKHPLLGQQSSAHSMSFSDKEERDSPLPNGRSISLMDLQDSYLAQGHPGPNSLNEASSRLGRVGSQASIGPVPPPHLHQPPVHPKVPQLRDNLPQSAPQVRRPIHPSLSQQRSLQPLSFQNPVYHLSNLHAQSTHSTQSTHSAQSLQPDSSSENLSTGSSRSASPSASGGRGATPRARIPSTSSVEEEFSRRSIQGQETPPPTARWHPPLADQHSGAQVVAVPRQSTTGTAHIVKVEQQSRGVGLVAANAGARTPRSLPHSTSIRSGSSANTEPIQQSGERSRQQSTCSKDGSVPGGRANKQVQSPVESVTMSPVERTAAWVLNNGQYEDEEEEGQSKDDAKHVEKYEQEISKLKERLRTSSRRLEEYERRLLAQEQQMQKLLLEYKTRLEDSEDRLRRQQEEKDSQMKSIICRLMAVEEELKRDHAEMQAVIDAKQKIIDAQEKRISSLDAANSRLMSALTQVKERYSMHNLRNGLSPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_005171322.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171322.4

ORF Insert Sequence:

ATGAGTTTAA CACGATTGGC CACACTGGAG GAACAAACGG GATCGAACAG GAGCCCATCG 
CGCTCTTCTT TTGGCAAGGA TAGTGTTCGT CGCTGGCGTC TTCTCAACAG GCCTTGGCTG 
AAGCACCGTT CAAATTCAGC TCCCGAGGAG CCCTTGGACA TGCATGACAG AAGGATCCTG 
GTTAGACAGG ACATGCGCAC ACAAAGCTTG ACATGGTCCA GAAGCAGCAG TACTAGGCCA 
TCCAATCCCC CTATCTCTAG GGTCCACAGT CTACCAGACC CTCAGCGGGT CACCAGCCGC 
GGTGTCCTCT TGGAGCATGC TAATCTTCAG AAGGACTGGT TATCTCGCAG CTCTTCGCCT 
TTTTTTAAGT CCACATCCGA ACTGGGGAGC GAAATTCACA CTGGGGCTCC ACCGCTCAGA 
CGGGTGTCAA GCTCCCATAT GTTTTCAGGG ATTCTGCTGT CCATGTCAAG ACGAATTAGC 
CGGATTTTAA ATACCTCTCC TGAGCCAGCA GTGGGAGAAC TTTCAGAAGA CGATGTGAAG 
GGTCCCGCCA GTCACCACTT CTCCTGCGGA CAGTCGCCTT TCACCGAGCC CTGCGTTTGG 
GAGAGGAAAT ACTGCATCCT GACTGATAGC CAGCTCATCC TGCTCAACAA GGAGGAAGAG 
ATGCCGTCAG AGGTGCATGA AAGCCCCTCA GCATCCTCTT CGAAGGGCCG CAGTTTGAGG 
CGGACAGTCA GTGTGCCTTC TGAAGGACCG TTCCCAGACT TCCCACCAGA GGGAGCCTCC 
ATGATAGCGG AAGCGTCCGC GGAGCGCTCT CCCAGACGGA GGAGCATATC AGGGCTAGGC 
AGCTCAGAGA AGAACATTTC CATCGATGGC CCCAACTCAT CTCCCTTCAA AGTGCCTGGT 
TTTTTAAGTA AACGACTGAA AGGATCCATC AAAAGGACGA AGAGCCAGAC TAAGCTGGAC 
CGCAACACAA GTTTCAGACT CCCATCGCTG CGACCAACAG AAAATGACAG GTCACGAGGC 
CTGCCCAAAT TGAAGGAGTC CTGTTCCCAT GAGTCTTTGC TGAGTCCTGG CAGCGCTGTT 
GAAGCTCTGG ATCTGAGTAT GGAGGAGGAT GTCTACATCA AACCTTTACA CAGCAGCATC 
CTAGGACAAG ATTTCTGCTT TGAGGTGGCG TACTCAGGTG GAAGTAAGTG CTTCAGCTGT 
TCCTCTGCTG CTGAACGGGA CAAATGGATG GAGAACCTCA AAAGAACTGT GCAACCTAAT 
AAGGATAATT GCCGGCGGGC GGAAAACGTC CTCCGCCTGT GGATCATTGA GGCAAAAGAC 
CTGCCACCAA AAAAGAAATA TTTCTGTGAG CTGTGTCTGG ATGACATGCT GTACGCTCGT 
ACCACCAGCA AAACCCGCTC CGACTGCCTG TTCTGGGGGG AACACTTTGA GTTTTCAGGC 
CTCCCGTCCA TGAAAAGCAT CACCATACAC ATCTACCGGG ATGTGGACAA GAAAAAGAAA 
AAGGACAAAA ACAACTATGT TGGCCTGGTG AACATCCCTG TGCATGGGGT GATGGGTAGG 
CAGTTTGTGG AGAAGTGGTA TCCAGTCAGC ACTCCCACCA CCAGCAAAGC CAAAGGTGGA 
GGCCCCTCCA TCCGCATCAA ATCCCGCTTT CAGACCATCT CAATTCTGCC CATGGAGCAG 
TACAAGGAGT TTGCGGAGTT TGTTACCAAT AACTATACCA TGTTGTGCTC TGTGTTGGAG 
CCGGTCATAA GCGTGAAGAA CAAAGAGGAG ATGGCCAGCG CTCTGGTGCA CATTCTGCAG 
AGCACGGGAC GGGCAAAGGA CTTCCTAACG GATTTGGTGA TGTCAGAAGT GGATCGCTGT 
GCTGACCATG ATGTGCTGAT CTTTAGAGAA AACACACTAG CCACCAAAGC CATTGAAGAA 
TACCTCAAAC TGGTGGGACA GAAGTACCTA CATGATGCAC TAGGGGAGTT TATTAAAGCC 
CTGTACGAGT CGGATGAAAA TTGTGAAGTA GACCAATCAA AGTGCACCAG CAACGAGCTT 
CCAGAGCATC AGAGTAACCT GAAGATGTGC TGCGAACTGG CCTTCTGCAA GATCATCAAC 
TCTTATTGTG TTTTTCCACG TGAACTGAAG GAGGTCTTTG CATCATGGAA GCAGCAGTGT 
CACGCTCGAG GGAAGCAGGA CATCAGTCAG CGTCTTATCA GCGCATCGCT CTTCCTGCGC 
TTTCTGTGTC CTGCCATCAT GTCCCCGTCA CTCTTTGGTC TCATGCAGGA ATATCCAGAT 
GACCGTACTT CTCGAACACT CACCCTCATT GCCAAAGTCA TTCAGAATCT TGCTAACTTT 
GCCAAATTCG GGAACAAAGA GGAGTACATG GCTTTCATGA ATGACTTTCT AGAGCACGAG 
TGGGCTGGTA TGACCCGTTT TCTGCTTGAG ATCTCGAATC TTGAGACAAT CTCCAACACC 
CCGGGCTTTG AGGGCTACAT TGACCTGGGC CGGGAGCTGT CAGTGCTTCA CTCGCTGCTC 
TGGGAGGTGG TGTCTCAGCT GGACAAGGCG ACGGTGGCCA AACTGGGGCC CCTGCCACGT 
ATCCTTGGCG ATATCACCCG CTCGCTGTCC AGCCCTACTC CCATCCAGCA GCAGCTCAGG 
CGCTTCCAGG ACCACAGCTC TGCTCATGAC ATCAGTGGAA GTGTGTCCTC AGGACTGCAG 
AGAATATTTG AAGACCCAGC AGACAGTGAA ATGAGGAGCA TCAAATCACC TGTCCAAGAG 
CACATGGAGG CTTTAGTCAG AGGAAAGCAT CCTTTACTGG GTCAACAGTC GTCCGCACAC 
AGCATGAGCT TCTCCGACAA AGAGGAAAGG GACAGTCCGC TCCCGAATGG ACGCAGTATA 
TCTTTAATGG ACTTGCAGGA CTCTTACCTT GCTCAGGGGC ATCCAGGTCC CAACTCGCTC 
AATGAAGCCT CATCTAGGTT AGGCAGAGTA GGCTCTCAGG CCTCCATCGG CCCTGTCCCT 
CCTCCACACC TCCACCAGCC TCCAGTCCAC CCGAAAGTTC CACAGCTGAG GGATAACTTA 
CCCCAGAGTG CACCACAGGT GCGGCGCCCG ATCCACCCAT CCCTTAGCCA GCAGCGCAGC 
CTACAACCGC TGTCTTTCCA AAACCCTGTT TACCACCTCA GCAACCTGCA CGCACAATCA 
ACACACTCAA CCCAGTCCAC ACACTCTGCC CAGTCCCTGC AGCCTGACTC TAGCTCAGAA 
AACCTGAGCA CAGGGAGCTC TCGGAGCGCC AGTCCTAGTG CCTCAGGTGG CCGGGGAGCA 
ACTCCCAGAG CCCGGATACC CTCCACAAGC AGCGTGGAGG AGGAATTTAG CCGAAGAAGC 
ATCCAGGGAC AGGAGACGCC GCCTCCAACG GCACGCTGGC ACCCTCCCCT GGCAGACCAG 
CACTCGGGAG CCCAGGTGGT GGCAGTGCCA AGGCAGAGTA CCACCGGTAC GGCACATATA 
GTGAAAGTGG AGCAGCAGAG TCGAGGAGTT GGGTTGGTGG CAGCAAATGC TGGAGCACGA 
ACCCCAAGGT CGCTCCCTCA CAGCACGTCC ATCCGCAGCG GCAGCAGCGC CAACACTGAA 
CCCATACAGC AGAGCGGTGA ACGATCAAGA CAGCAGTCCA CGTGCTCCAA AGATGGTTCA 
GTACCAGGAG GACGAGCCAA CAAACAGGTC CAGTCACCTG TAGAGTCTGT CACCATGTCC 
CCAGTGGAGC GAACAGCTGC GTGGGTTTTG AATAATGGCC AGTATGAAGA TGAGGAAGAG 
GAAGGGCAGA GTAAAGACGA TGCCAAACAT GTGGAGAAGT ATGAACAGGA AATCTCGAAG 
CTAAAGGAGC GTCTGCGGAC GTCTAGTCGG CGGCTGGAGG AGTATGAGAG ACGGTTACTG 
GCTCAGGAGC AGCAGATGCA GAAGCTGCTC TTAGAATACA AGACTCGACT GGAGGACAGT 
GAGGACCGTC TGCGAAGACA ACAGGAGGAA AAAGACAGCC AGATGAAGAG TATTATCTGC 
AGGCTGATGG CCGTGGAGGA AGAGCTGAAG AGAGACCATG CAGAGATGCA GGCAGTCATA 
GATGCCAAAC AGAAGATAAT CGATGCACAG GAGAAGAGGA TCAGCTCTCT GGATGCTGCT 
AACTCTCGGC TGATGAGCGC TCTGACTCAG GTGAAGGAGC GCTACAGCAT GCACAACCTC 
CGGAACGGCC TGTCACCCAC CAACCCCACC AAACTCTCCA TCACAGAGAA CGGGGAGTTC 
AAGAATAGCA GCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasal2 ( XM_005171322.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171379.1 Danio rerio RAS protein activator like 2 (rasal2), transcript variant X2, mRNA.

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CloneID	ODa07237
Clone ID Related Accession (Same CDS sequence)	XM_005171322.4 , XM_005171322.3
Accession Version	XM_005171322.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras GTPase-activating protein nGAP isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171322.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTTAA CACGATTGGC CACACTGGAG GAACAAACGG GATCGAACAG GAGCCCATCG 
CGCTCTTCTT TTGGCAAGGA TAGTGTTCGT CGCTGGCGTC TTCTCAACAG GCCTTGGCTG 
AAGCACCGTT CAAATTCAGC TCCCGAGGAG CCCTTGGACA TGCATGACAG AAGGATCCTG 
GTTAGACAGG ACATGCGCAC ACAAAGCTTG ACATGGTCCA GAAGCAGCAG TACTAGGCCA 
TCCAATCCCC CTATCTCTAG GGTCCACAGT CTACCAGACC CTCAGCGGGT CACCAGCCGC 
GGTGTCCTCT TGGAGCATGC TAATCTTCAG AAGGACTGGT TATCTCGCAG CTCTTCGCCT 
TTTTTTAAGT CCACATCCGA ACTGGGGAGC GAAATTCACA CTGGGGCTCC ACCGCTCAGA 
CGGGTGTCAA GCTCCCATAT GTTTTCAGGG ATTCTGCTGT CCATGTCAAG ACGAATTAGC 
CGGATTTTAA ATACCTCTCC TGAGCCAGCA GTGGGAGAAC TTTCAGAAGA CGATGTGAAG 
GGTCCCGCCA GTCACCACTT CTCCTGCGGA CAGTCGCCTT TCACCGAGCC CTGCGTTTGG 
GAGAGGAAAT ACTGCATCCT GACTGATAGC CAGCTCATCC TGCTCAACAA GGAGGAAGAG 
ATGCCGTCAG AGGTGCATGA AAGCCCCTCA GCATCCTCTT CGAAGGGCCG CAGTTTGAGG 
CGGACAGTCA GTGTGCCTTC TGAAGGACCG TTCCCAGACT TCCCACCAGA GGGAGCCTCC 
ATGATAGCGG AAGCGTCCGC GGAGCGCTCT CCCAGACGGA GGAGCATATC AGGGCTAGGC 
AGCTCAGAGA AGAACATTTC CATCGATGGC CCCAACTCAT CTCCCTTCAA AGTGCCTGGT 
TTTTTAAGTA AACGACTGAA AGGATCCATC AAAAGGACGA AGAGCCAGAC TAAGCTGGAC 
CGCAACACAA GTTTCAGACT CCCATCGCTG CGACCAACAG AAAATGACAG GTCACGAGGC 
CTGCCCAAAT TGAAGGAGTC CTGTTCCCAT GAGTCTTTGC TGAGTCCTGG CAGCGCTGTT 
GAAGCTCTGG ATCTGAGTAT GGAGGAGGAT GTCTACATCA AACCTTTACA CAGCAGCATC 
CTAGGACAAG ATTTCTGCTT TGAGGTGGCG TACTCAGGTG GAAGTAAGTG CTTCAGCTGT 
TCCTCTGCTG CTGAACGGGA CAAATGGATG GAGAACCTCA AAAGAACTGT GCAACCTAAT 
AAGGATAATT GCCGGCGGGC GGAAAACGTC CTCCGCCTGT GGATCATTGA GGCAAAAGAC 
CTGCCACCAA AAAAGAAATA TTTCTGTGAG CTGTGTCTGG ATGACATGCT GTACGCTCGT 
ACCACCAGCA AAACCCGCTC CGACTGCCTG TTCTGGGGGG AACACTTTGA GTTTTCAGGC 
CTCCCGTCCA TGAAAAGCAT CACCATACAC ATCTACCGGG ATGTGGACAA GAAAAAGAAA 
AAGGACAAAA ACAACTATGT TGGCCTGGTG AACATCCCTG TGCATGGGGT GATGGGTAGG 
CAGTTTGTGG AGAAGTGGTA TCCAGTCAGC ACTCCCACCA CCAGCAAAGC CAAAGGTGGA 
GGCCCCTCCA TCCGCATCAA ATCCCGCTTT CAGACCATCT CAATTCTGCC CATGGAGCAG 
TACAAGGAGT TTGCGGAGTT TGTTACCAAT AACTATACCA TGTTGTGCTC TGTGTTGGAG 
CCGGTCATAA GCGTGAAGAA CAAAGAGGAG ATGGCCAGCG CTCTGGTGCA CATTCTGCAG 
AGCACGGGAC GGGCAAAGGA CTTCCTAACG GATTTGGTGA TGTCAGAAGT GGATCGCTGT 
GCTGACCATG ATGTGCTGAT CTTTAGAGAA AACACACTAG CCACCAAAGC CATTGAAGAA 
TACCTCAAAC TGGTGGGACA GAAGTACCTA CATGATGCAC TAGGGGAGTT TATTAAAGCC 
CTGTACGAGT CGGATGAAAA TTGTGAAGTA GACCAATCAA AGTGCACCAG CAACGAGCTT 
CCAGAGCATC AGAGTAACCT GAAGATGTGC TGCGAACTGG CCTTCTGCAA GATCATCAAC 
TCTTATTGTG TTTTTCCACG TGAACTGAAG GAGGTCTTTG CATCATGGAA GCAGCAGTGT 
CACGCTCGAG GGAAGCAGGA CATCAGTCAG CGTCTTATCA GCGCATCGCT CTTCCTGCGC 
TTTCTGTGTC CTGCCATCAT GTCCCCGTCA CTCTTTGGTC TCATGCAGGA ATATCCAGAT 
GACCGTACTT CTCGAACACT CACCCTCATT GCCAAAGTCA TTCAGAATCT TGCTAACTTT 
GCCAAATTCG GGAACAAAGA GGAGTACATG GCTTTCATGA ATGACTTTCT AGAGCACGAG 
TGGGCTGGTA TGACCCGTTT TCTGCTTGAG ATCTCGAATC TTGAGACAAT CTCCAACACC 
CCGGGCTTTG AGGGCTACAT TGACCTGGGC CGGGAGCTGT CAGTGCTTCA CTCGCTGCTC 
TGGGAGGTGG TGTCTCAGCT GGACAAGGCG ACGGTGGCCA AACTGGGGCC CCTGCCACGT 
ATCCTTGGCG ATATCACCCG CTCGCTGTCC AGCCCTACTC CCATCCAGCA GCAGCTCAGG 
CGCTTCCAGG ACCACAGCTC TGCTCATGAC ATCAGTGGAA GTGTGTCCTC AGGACTGCAG 
AGAATATTTG AAGACCCAGC AGACAGTGAA ATGAGGAGCA TCAAATCACC TGTCCAAGAG 
CACATGGAGG CTTTAGTCAG AGGAAAGCAT CCTTTACTGG GTCAACAGTC GTCCGCACAC 
AGCATGAGCT TCTCCGACAA AGAGGAAAGG GACAGTCCGC TCCCGAATGG ACGCAGTATA 
TCTTTAATGG ACTTGCAGGA CTCTTACCTT GCTCAGGGGC ATCCAGGTCC CAACTCGCTC 
AATGAAGCCT CATCTAGGTT AGGCAGAGTA GGCTCTCAGG CCTCCATCGG CCCTGTCCCT 
CCTCCACACC TCCACCAGCC TCCAGTCCAC CCGAAAGTTC CACAGCTGAG GGATAACTTA 
CCCCAGAGTG CACCACAGGT GCGGCGCCCG ATCCACCCAT CCCTTAGCCA GCAGCGCAGC 
CTACAACCGC TGTCTTTCCA AAACCCTGTT TACCACCTCA GCAACCTGCA CGCACAATCA 
ACACACTCAA CCCAGTCCAC ACACTCTGCC CAGTCCCTGC AGCCTGACTC TAGCTCAGAA 
AACCTGAGCA CAGGGAGCTC TCGGAGCGCC AGTCCTAGTG CCTCAGGTGG CCGGGGAGCA 
ACTCCCAGAG CCCGGATACC CTCCACAAGC AGCGTGGAGG AGGAATTTAG CCGAAGAAGC 
ATCCAGGGAC AGGAGACGCC GCCTCCAACG GCACGCTGGC ACCCTCCCCT GGCAGACCAG 
CACTCGGGAG CCCAGGTGGT GGCAGTGCCA AGGCAGAGTA CCACCGGTAC GGCACATATA 
GTGAAAGTGG AGCAGCAGAG TCGAGGAGTT GGGTTGGTGG CAGCAAATGC TGGAGCACGA 
ACCCCAAGGT CGCTCCCTCA CAGCACGTCC ATCCGCAGCG GCAGCAGCGC CAACACTGAA 
CCCATACAGC AGAGCGGTGA ACGATCAAGA CAGCAGTCCA CGTGCTCCAA AGATGGTTCA 
GTACCAGGAG GACGAGCCAA CAAACAGGTC CAGTCACCTG TAGAGTCTGT CACCATGTCC 
CCAGTGGAGC GAACAGCTGC GTGGGTTTTG AATAATGGCC AGTATGAAGA TGAGGAAGAG 
GAAGGGCAGA GTAAAGACGA TGCCAAACAT GTGGAGAAGT ATGAACAGGA AATCTCGAAG 
CTAAAGGAGC GTCTGCGGAC GTCTAGTCGG CGGCTGGAGG AGTATGAGAG ACGGTTACTG 
GCTCAGGAGC AGCAGATGCA GAAGCTGCTC TTAGAATACA AGACTCGACT GGAGGACAGT 
GAGGACCGTC TGCGAAGACA ACAGGAGGAA AAAGACAGCC AGATGAAGAG TATTATCTGC 
AGGCTGATGG CCGTGGAGGA AGAGCTGAAG AGAGACCATG CAGAGATGCA GGCAGTCATA 
GATGCCAAAC AGAAGATAAT CGATGCACAG GAGAAGAGGA TCAGCTCTCT GGATGCTGCT 
AACTCTCGGC TGATGAGCGC TCTGACTCAG GTGAAGGAGC GCTACAGCAT GCACAACCTC 
CGGAACGGCC TGTCACCCAC CAACCCCACC AAACTCTCCA TCACAGAGAA CGGGGAGTTC 
AAGAATAGCA GCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171379.1
CDS	16..4293
Translation	MSLTRLATLEEQTGSNRSPSRSSFGKDSVRRWRLLNRPWLKHRSNSAPEEPLDMHDRRILVRQDMRTQSLTWSRSSSTRPSNPPISRVHSLPDPQRVTSRGVLLEHANLQKDWLSRSSSPFFKSTSELGSEIHTGAPPLRRVSSSHMFSGILLSMSRRISRILNTSPEPAVGELSEDDVKGPASHHFSCGQSPFTEPCVWERKYCILTDSQLILLNKEEEMPSEVHESPSASSSKGRSLRRTVSVPSEGPFPDFPPEGASMIAEASAERSPRRRSISGLGSSEKNISIDGPNSSPFKVPGFLSKRLKGSIKRTKSQTKLDRNTSFRLPSLRPTENDRSRGLPKLKESCSHESLLSPGSAVEALDLSMEEDVYIKPLHSSILGQDFCFEVAYSGGSKCFSCSSAAERDKWMENLKRTVQPNKDNCRRAENVLRLWIIEAKDLPPKKKYFCELCLDDMLYARTTSKTRSDCLFWGEHFEFSGLPSMKSITIHIYRDVDKKKKKDKNNYVGLVNIPVHGVMGRQFVEKWYPVSTPTTSKAKGGGPSIRIKSRFQTISILPMEQYKEFAEFVTNNYTMLCSVLEPVISVKNKEEMASALVHILQSTGRAKDFLTDLVMSEVDRCADHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDQSKCTSNELPEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCHARGKQDISQRLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWAGMTRFLLEISNLETISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRILGDITRSLSSPTPIQQQLRRFQDHSSAHDISGSVSSGLQRIFEDPADSEMRSIKSPVQEHMEALVRGKHPLLGQQSSAHSMSFSDKEERDSPLPNGRSISLMDLQDSYLAQGHPGPNSLNEASSRLGRVGSQASIGPVPPPHLHQPPVHPKVPQLRDNLPQSAPQVRRPIHPSLSQQRSLQPLSFQNPVYHLSNLHAQSTHSTQSTHSAQSLQPDSSSENLSTGSSRSASPSASGGRGATPRARIPSTSSVEEEFSRRSIQGQETPPPTARWHPPLADQHSGAQVVAVPRQSTTGTAHIVKVEQQSRGVGLVAANAGARTPRSLPHSTSIRSGSSANTEPIQQSGERSRQQSTCSKDGSVPGGRANKQVQSPVESVTMSPVERTAAWVLNNGQYEDEEEEGQSKDDAKHVEKYEQEISKLKERLRTSSRRLEEYERRLLAQEQQMQKLLLEYKTRLEDSEDRLRRQQEEKDSQMKSIICRLMAVEEELKRDHAEMQAVIDAKQKIIDAQEKRISSLDAANSRLMSALTQVKERYSMHNLRNGLSPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_005171322.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171322.3

ORF Insert Sequence:

ATGAGTTTAA CACGATTGGC CACACTGGAG GAACAAACGG GATCGAACAG GAGCCCATCG 
CGCTCTTCTT TTGGCAAGGA TAGTGTTCGT CGCTGGCGTC TTCTCAACAG GCCTTGGCTG 
AAGCACCGTT CAAATTCAGC TCCCGAGGAG CCCTTGGACA TGCATGACAG AAGGATCCTG 
GTTAGACAGG ACATGCGCAC ACAAAGCTTG ACATGGTCCA GAAGCAGCAG TACTAGGCCA 
TCCAATCCCC CTATCTCTAG GGTCCACAGT CTACCAGACC CTCAGCGGGT CACCAGCCGC 
GGTGTCCTCT TGGAGCATGC TAATCTTCAG AAGGACTGGT TATCTCGCAG CTCTTCGCCT 
TTTTTTAAGT CCACATCCGA ACTGGGGAGC GAAATTCACA CTGGGGCTCC ACCGCTCAGA 
CGGGTGTCAA GCTCCCATAT GTTTTCAGGG ATTCTGCTGT CCATGTCAAG ACGAATTAGC 
CGGATTTTAA ATACCTCTCC TGAGCCAGCA GTGGGAGAAC TTTCAGAAGA CGATGTGAAG 
GGTCCCGCCA GTCACCACTT CTCCTGCGGA CAGTCGCCTT TCACCGAGCC CTGCGTTTGG 
GAGAGGAAAT ACTGCATCCT GACTGATAGC CAGCTCATCC TGCTCAACAA GGAGGAAGAG 
ATGCCGTCAG AGGTGCATGA AAGCCCCTCA GCATCCTCTT CGAAGGGCCG CAGTTTGAGG 
CGGACAGTCA GTGTGCCTTC TGAAGGACCG TTCCCAGACT TCCCACCAGA GGGAGCCTCC 
ATGATAGCGG AAGCGTCCGC GGAGCGCTCT CCCAGACGGA GGAGCATATC AGGGCTAGGC 
AGCTCAGAGA AGAACATTTC CATCGATGGC CCCAACTCAT CTCCCTTCAA AGTGCCTGGT 
TTTTTAAGTA AACGACTGAA AGGATCCATC AAAAGGACGA AGAGCCAGAC TAAGCTGGAC 
CGCAACACAA GTTTCAGACT CCCATCGCTG CGACCAACAG AAAATGACAG GTCACGAGGC 
CTGCCCAAAT TGAAGGAGTC CTGTTCCCAT GAGTCTTTGC TGAGTCCTGG CAGCGCTGTT 
GAAGCTCTGG ATCTGAGTAT GGAGGAGGAT GTCTACATCA AACCTTTACA CAGCAGCATC 
CTAGGACAAG ATTTCTGCTT TGAGGTGGCG TACTCAGGTG GAAGTAAGTG CTTCAGCTGT 
TCCTCTGCTG CTGAACGGGA CAAATGGATG GAGAACCTCA AAAGAACTGT GCAACCTAAT 
AAGGATAATT GCCGGCGGGC GGAAAACGTC CTCCGCCTGT GGATCATTGA GGCAAAAGAC 
CTGCCACCAA AAAAGAAATA TTTCTGTGAG CTGTGTCTGG ATGACATGCT GTACGCTCGT 
ACCACCAGCA AAACCCGCTC CGACTGCCTG TTCTGGGGGG AACACTTTGA GTTTTCAGGC 
CTCCCGTCCA TGAAAAGCAT CACCATACAC ATCTACCGGG ATGTGGACAA GAAAAAGAAA 
AAGGACAAAA ACAACTATGT TGGCCTGGTG AACATCCCTG TGCATGGGGT GATGGGTAGG 
CAGTTTGTGG AGAAGTGGTA TCCAGTCAGC ACTCCCACCA CCAGCAAAGC CAAAGGTGGA 
GGCCCCTCCA TCCGCATCAA ATCCCGCTTT CAGACCATCT CAATTCTGCC CATGGAGCAG 
TACAAGGAGT TTGCGGAGTT TGTTACCAAT AACTATACCA TGTTGTGCTC TGTGTTGGAG 
CCGGTCATAA GCGTGAAGAA CAAAGAGGAG ATGGCCAGCG CTCTGGTGCA CATTCTGCAG 
AGCACGGGAC GGGCAAAGGA CTTCCTAACG GATTTGGTGA TGTCAGAAGT GGATCGCTGT 
GCTGACCATG ATGTGCTGAT CTTTAGAGAA AACACACTAG CCACCAAAGC CATTGAAGAA 
TACCTCAAAC TGGTGGGACA GAAGTACCTA CATGATGCAC TAGGGGAGTT TATTAAAGCC 
CTGTACGAGT CGGATGAAAA TTGTGAAGTA GACCAATCAA AGTGCACCAG CAACGAGCTT 
CCAGAGCATC AGAGTAACCT GAAGATGTGC TGCGAACTGG CCTTCTGCAA GATCATCAAC 
TCTTATTGTG TTTTTCCACG TGAACTGAAG GAGGTCTTTG CATCATGGAA GCAGCAGTGT 
CACGCTCGAG GGAAGCAGGA CATCAGTCAG CGTCTTATCA GCGCATCGCT CTTCCTGCGC 
TTTCTGTGTC CTGCCATCAT GTCCCCGTCA CTCTTTGGTC TCATGCAGGA ATATCCAGAT 
GACCGTACTT CTCGAACACT CACCCTCATT GCCAAAGTCA TTCAGAATCT TGCTAACTTT 
GCCAAATTCG GGAACAAAGA GGAGTACATG GCTTTCATGA ATGACTTTCT AGAGCACGAG 
TGGGCTGGTA TGACCCGTTT TCTGCTTGAG ATCTCGAATC TTGAGACAAT CTCCAACACC 
CCGGGCTTTG AGGGCTACAT TGACCTGGGC CGGGAGCTGT CAGTGCTTCA CTCGCTGCTC 
TGGGAGGTGG TGTCTCAGCT GGACAAGGCG ACGGTGGCCA AACTGGGGCC CCTGCCACGT 
ATCCTTGGCG ATATCACCCG CTCGCTGTCC AGCCCTACTC CCATCCAGCA GCAGCTCAGG 
CGCTTCCAGG ACCACAGCTC TGCTCATGAC ATCAGTGGAA GTGTGTCCTC AGGACTGCAG 
AGAATATTTG AAGACCCAGC AGACAGTGAA ATGAGGAGCA TCAAATCACC TGTCCAAGAG 
CACATGGAGG CTTTAGTCAG AGGAAAGCAT CCTTTACTGG GTCAACAGTC GTCCGCACAC 
AGCATGAGCT TCTCCGACAA AGAGGAAAGG GACAGTCCGC TCCCGAATGG ACGCAGTATA 
TCTTTAATGG ACTTGCAGGA CTCTTACCTT GCTCAGGGGC ATCCAGGTCC CAACTCGCTC 
AATGAAGCCT CATCTAGGTT AGGCAGAGTA GGCTCTCAGG CCTCCATCGG CCCTGTCCCT 
CCTCCACACC TCCACCAGCC TCCAGTCCAC CCGAAAGTTC CACAGCTGAG GGATAACTTA 
CCCCAGAGTG CACCACAGGT GCGGCGCCCG ATCCACCCAT CCCTTAGCCA GCAGCGCAGC 
CTACAACCGC TGTCTTTCCA AAACCCTGTT TACCACCTCA GCAACCTGCA CGCACAATCA 
ACACACTCAA CCCAGTCCAC ACACTCTGCC CAGTCCCTGC AGCCTGACTC TAGCTCAGAA 
AACCTGAGCA CAGGGAGCTC TCGGAGCGCC AGTCCTAGTG CCTCAGGTGG CCGGGGAGCA 
ACTCCCAGAG CCCGGATACC CTCCACAAGC AGCGTGGAGG AGGAATTTAG CCGAAGAAGC 
ATCCAGGGAC AGGAGACGCC GCCTCCAACG GCACGCTGGC ACCCTCCCCT GGCAGACCAG 
CACTCGGGAG CCCAGGTGGT GGCAGTGCCA AGGCAGAGTA CCACCGGTAC GGCACATATA 
GTGAAAGTGG AGCAGCAGAG TCGAGGAGTT GGGTTGGTGG CAGCAAATGC TGGAGCACGA 
ACCCCAAGGT CGCTCCCTCA CAGCACGTCC ATCCGCAGCG GCAGCAGCGC CAACACTGAA 
CCCATACAGC AGAGCGGTGA ACGATCAAGA CAGCAGTCCA CGTGCTCCAA AGATGGTTCA 
GTACCAGGAG GACGAGCCAA CAAACAGGTC CAGTCACCTG TAGAGTCTGT CACCATGTCC 
CCAGTGGAGC GAACAGCTGC GTGGGTTTTG AATAATGGCC AGTATGAAGA TGAGGAAGAG 
GAAGGGCAGA GTAAAGACGA TGCCAAACAT GTGGAGAAGT ATGAACAGGA AATCTCGAAG 
CTAAAGGAGC GTCTGCGGAC GTCTAGTCGG CGGCTGGAGG AGTATGAGAG ACGGTTACTG 
GCTCAGGAGC AGCAGATGCA GAAGCTGCTC TTAGAATACA AGACTCGACT GGAGGACAGT 
GAGGACCGTC TGCGAAGACA ACAGGAGGAA AAAGACAGCC AGATGAAGAG TATTATCTGC 
AGGCTGATGG CCGTGGAGGA AGAGCTGAAG AGAGACCATG CAGAGATGCA GGCAGTCATA 
GATGCCAAAC AGAAGATAAT CGATGCACAG GAGAAGAGGA TCAGCTCTCT GGATGCTGCT 
AACTCTCGGC TGATGAGCGC TCTGACTCAG GTGAAGGAGC GCTACAGCAT GCACAACCTC 
CGGAACGGCC TGTCACCCAC CAACCCCACC AAACTCTCCA TCACAGAGAA CGGGGAGTTC 
AAGAATAGCA GCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasal2 ( XM_001332652.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001332688.5 Danio rerio RAS protein activator like 2 (rasal2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa07239
Clone ID Related Accession (Same CDS sequence)	XM_001332652.8 , XM_001332652.7
Accession Version	XM_001332652.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras GTPase-activating protein nGAP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001332652.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ACTCAGTGAT ACCAAACCAA CACAAACGAA CGGAAAACAT TGTGATTGAG 
CCTCTTCTGC CACGCGCTTG GAAACCCAGC GGACTGGAGT CTTTACCTGG ACGTTACCGT 
GGAGGTGTAT CGAGCATGGA GACGGATTCG GTAACCGGTG GCGACGTGGA CTCGGTTCAG 
GGAGGCATGC TTTCTTTAGA CCCTGTGGTC GATGGGATTT TGATGGACTC GTTTTGCCAG 
CAGCAGGGAT GGGTGCGGGT GTACGATGTG AAGGGTCCCG CCAGTCACCA CTTCTCCTGC 
GGACAGTCGC CTTTCACCGA GCCCTGCGTT TGGGAGAGGA AATACTGCAT CCTGACTGAT 
AGCCAGCTCA TCCTGCTCAA CAAGGAGGAA GAGATGCCGT CAGAGGTGCA TGAAAGCCCC 
TCAGCATCCT CTTCGAAGGG CCGCAGTTTG AGGCGGACAG TCAGTGTGCC TTCTGAAGGA 
CCGTTCCCAG ACTTCCCACC AGAGGGAGCC TCCATGATAG CGGAAGCGTC CGCGGAGCGC 
TCTCCCAGAC GGAGGAGCAT ATCAGGGCTA GGCAGCTCAG AGAAGAACAT TTCCATCGAT 
GGCCCCAACT CATCTCCCTT CAAAGTGCCT GGTTTTTTAA GTAAACGACT GAAAGGATCC 
ATCAAAAGGA CGAAGAGCCA GACTAAGCTG GACCGCAACA CAAGTTTCAG ACTCCCATCG 
CTGCGACCAA CAGAAAATGA CAGGTCACGA GGCCTGCCCA AATTGAAGGA GTCCTGTTCC 
CATGAGTCTT TGCTGAGTCC TGGCAGCGCT GTTGAAGCTC TGGATCTGAG TATGGAGGAG 
GATGTCTACA TCAAACCTTT ACACAGCAGC ATCCTAGGAC AAGATTTCTG CTTTGAGGTG 
GCGTACTCAG GTGGAAGTAA GTGCTTCAGC TGTTCCTCTG CTGCTGAACG GGACAAATGG 
ATGGAGAACC TCAAAAGAAC TGTGCAACCT AATAAGGATA ATTGCCGGCG GGCGGAAAAC 
GTCCTCCGCC TGTGGATCAT TGAGGCAAAA GACCTGCCAC CAAAAAAGAA ATATTTCTGT 
GAGCTGTGTC TGGATGACAT GCTGTACGCT CGTACCACCA GCAAAACCCG CTCCGACTGC 
CTGTTCTGGG GGGAACACTT TGAGTTTTCA GGCCTCCCGT CCATGAAAAG CATCACCATA 
CACATCTACC GGGATGTGGA CAAGAAAAAG AAAAAGGACA AAAACAACTA TGTTGGCCTG 
GTGAACATCC CTGTGCATGG GGTGATGGGT AGGCAGTTTG TGGAGAAGTG GTATCCAGTC 
AGCACTCCCA CCACCAGCAA AGCCAAAGGT GGAGGCCCCT CCATCCGCAT CAAATCCCGC 
TTTCAGACCA TCTCAATTCT GCCCATGGAG CAGTACAAGG AGTTTGCGGA GTTTGTTACC 
AATAACTATA CCATGTTGTG CTCTGTGTTG GAGCCGGTCA TAAGCGTGAA GAACAAAGAG 
GAGATGGCCA GCGCTCTGGT GCACATTCTG CAGAGCACGG GACGGGCAAA GGACTTCCTA 
ACGGATTTGG TGATGTCAGA AGTGGATCGC TGTGCTGACC ATGATGTGCT GATCTTTAGA 
GAAAACACAC TAGCCACCAA AGCCATTGAA GAATACCTCA AACTGGTGGG ACAGAAGTAC 
CTACATGATG CACTAGGGGA GTTTATTAAA GCCCTGTACG AGTCGGATGA AAATTGTGAA 
GTAGACCAAT CAAAGTGCAC CAGCAACGAG CTTCCAGAGC ATCAGAGTAA CCTGAAGATG 
TGCTGCGAAC TGGCCTTCTG CAAGATCATC AACTCTTATT GTGTTTTTCC ACGTGAACTG 
AAGGAGGTCT TTGCATCATG GAAGCAGCAG TGTCACGCTC GAGGGAAGCA GGACATCAGT 
CAGCGTCTTA TCAGCGCATC GCTCTTCCTG CGCTTTCTGT GTCCTGCCAT CATGTCCCCG 
TCACTCTTTG GTCTCATGCA GGAATATCCA GATGACCGTA CTTCTCGAAC ACTCACCCTC 
ATTGCCAAAG TCATTCAGAA TCTTGCTAAC TTTGCCAAAT TCGGGAACAA AGAGGAGTAC 
ATGGCTTTCA TGAATGACTT TCTAGAGCAC GAGTGGGCTG GTATGACCCG TTTTCTGCTT 
GAGATCTCGA ATCTTGAGAC AATCTCCAAC ACCCCGGGCT TTGAGGGCTA CATTGACCTG 
GGCCGGGAGC TGTCAGTGCT TCACTCGCTG CTCTGGGAGG TGGTGTCTCA GCTGGACAAG 
GGTGACAATT CCTTCCTGCA GGCGACGGTG GCCAAACTGG GGCCCCTGCC ACGTATCCTT 
GGCGATATCA CCCGCTCGCT GTCCAGCCCT ACTCCCATCC AGCAGCAGCT CAGGCGCTTC 
CAGGACCACA GCTCTGCTCA TGACATCAGT GGAAGTGTGT CCTCAGGACT GCAGAGAATA 
TTTGAAGACC CAGCAGACAG TGAAATGAGG AGCATCAAAT CACCTGTCCA AGAGCACATG 
GAGGCTTTAG TCAGAGGAAA GCATCCTTTA CTGGGTCAAC AGTCGTCCGC ACACAGCATG 
AGCTTCTCCG ACAAAGAGGA AAGGGACAGT CCGCTCCCGA ATGGACGCAG TATATCTTTA 
ATGGACTTGC AGGACTCTTA CCTTGCTCAG GGGCATCCAG GTCCCAACTC GCTCAATGAA 
GCCTCATCTA GGTTAGGCAG AGTAGGCTCT CAGGCCTCCA TCGGCCCTGT CCCTCCTCCA 
CACCTCCACC AGCCTCCAGT CCACCCGAAA GTTCCACAGC TGAGGGATAA CTTACCCCAG 
AGTGCACCAC AGGTGCGGCG CCCGATCCAC CCATCCCTTA GCCAGCAGCG CAGCCTACAA 
CCGCTGTCTT TCCAAAACCC TGTTTACCAC CTCAGCAACC TGCACGCACA ATCAACACAC 
TCAACCCAGT CCACACACTC TGCCCAGTCC CTGCAGCCTG ACTCTAGCTC AGAAAACCTG 
AGCACAGGGA GCTCTCGGAG CGCCAGTCCT AGTGCCTCAG GTGGCCGGGG AGCAACTCCC 
AGAGCCCGGA TACCCTCCAC AAGCAGCGTG GAGGAGGAAT TTAGCCGAAG AAGCATCCAG 
GGACAGGAGA CGCCGCCTCC AACGGCACGC TGGCACCCTC CCCTGGCAGA CCAGCACTCG 
GGAGCCCAGG TGGTGGCAGT GCCAAGGCAG AGTACCACCG GTACGGCACA TATAGTGAAA 
GTGGAGCAGC AGAGTCGAGG AGTTGGGTTG GTGGCAGCAA ATGCTGGAGC ACGAACCCCA 
AGGTCGCTCC CTCACAGCAC GTCCATCCGC AGCGGCAGCA GCGCCAACAC TGAACCCATA 
CAGCAGAGCG GTGAACGATC AAGACAGCAG TCCACGTGCT CCAAAGATGG TTCAGTACCA 
GGAGGACGAG CCAACAAACA GGTCCAGTCA CCTGTAGAGT CTGTCACCAT GTCCCCAGTG 
GAGCGAACAG CTGCGTGGGT TTTGAATAAT GGCCAGTATG AAGATGAGGA AGAGGAAGGG 
CAGAGTAAAG ACGATGCCAA ACATGTGGAG AAGTATGAAC AGGAAATCTC GAAGCTAAAG 
GAGCGTCTGC GGACGTCTAG TCGGCGGCTG GAGGAGTATG AGAGACGGTT ACTGGCTCAG 
GAGCAGCAGA TGCAGAAGCT GCTCTTAGAA TACAAGACTC GACTGGAGGA CAGTGAGGAC 
CGTCTGCGAA GACAACAGGA GGAAAAAGAC AGCCAGATGA AGAGTATTAT CTGCAGGCTG 
ATGGCCGTGG AGGAAGAGCT GAAGAGAGAC CATGCAGAGA TGCAGGCAGT CATAGATGCC 
AAACAGAAGA TAATCGATGC ACAGGAGAAG AGGATCAGCT CTCTGGATGC TGCTAACTCT 
CGGCTGATGA GCGCTCTGAC TCAGGTGAAG GAGCGCTACA GCATGCACAA CCTCCGGAAC 
GGCCTGTCAC CCACCAACCC CACCAAACTC TCCATCACAG AGAACGGGGA GTTCAAGAAT 
AGCAGCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001332688.5
CDS	659..4690
Translation	MVVNSVIPNQHKRTENIVIEPLLPRAWKPSGLESLPGRYRGGVSSMETDSVTGGDVDSVQGGMLSLDPVVDGILMDSFCQQQGWVRVYDVKGPASHHFSCGQSPFTEPCVWERKYCILTDSQLILLNKEEEMPSEVHESPSASSSKGRSLRRTVSVPSEGPFPDFPPEGASMIAEASAERSPRRRSISGLGSSEKNISIDGPNSSPFKVPGFLSKRLKGSIKRTKSQTKLDRNTSFRLPSLRPTENDRSRGLPKLKESCSHESLLSPGSAVEALDLSMEEDVYIKPLHSSILGQDFCFEVAYSGGSKCFSCSSAAERDKWMENLKRTVQPNKDNCRRAENVLRLWIIEAKDLPPKKKYFCELCLDDMLYARTTSKTRSDCLFWGEHFEFSGLPSMKSITIHIYRDVDKKKKKDKNNYVGLVNIPVHGVMGRQFVEKWYPVSTPTTSKAKGGGPSIRIKSRFQTISILPMEQYKEFAEFVTNNYTMLCSVLEPVISVKNKEEMASALVHILQSTGRAKDFLTDLVMSEVDRCADHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDQSKCTSNELPEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCHARGKQDISQRLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWAGMTRFLLEISNLETISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKGDNSFLQATVAKLGPLPRILGDITRSLSSPTPIQQQLRRFQDHSSAHDISGSVSSGLQRIFEDPADSEMRSIKSPVQEHMEALVRGKHPLLGQQSSAHSMSFSDKEERDSPLPNGRSISLMDLQDSYLAQGHPGPNSLNEASSRLGRVGSQASIGPVPPPHLHQPPVHPKVPQLRDNLPQSAPQVRRPIHPSLSQQRSLQPLSFQNPVYHLSNLHAQSTHSTQSTHSAQSLQPDSSSENLSTGSSRSASPSASGGRGATPRARIPSTSSVEEEFSRRSIQGQETPPPTARWHPPLADQHSGAQVVAVPRQSTTGTAHIVKVEQQSRGVGLVAANAGARTPRSLPHSTSIRSGSSANTEPIQQSGERSRQQSTCSKDGSVPGGRANKQVQSPVESVTMSPVERTAAWVLNNGQYEDEEEEGQSKDDAKHVEKYEQEISKLKERLRTSSRRLEEYERRLLAQEQQMQKLLLEYKTRLEDSEDRLRRQQEEKDSQMKSIICRLMAVEEELKRDHAEMQAVIDAKQKIIDAQEKRISSLDAANSRLMSALTQVKERYSMHNLRNGLSPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_001332652.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001332652.8

ORF Insert Sequence:

ATGGTTGTAA ACTCAGTGAT ACCAAACCAA CACAAACGAA CGGAAAACAT TGTGATTGAG 
CCTCTTCTGC CACGCGCTTG GAAACCCAGC GGACTGGAGT CTTTACCTGG ACGTTACCGT 
GGAGGTGTAT CGAGCATGGA GACGGATTCG GTAACCGGTG GCGACGTGGA CTCGGTTCAG 
GGAGGCATGC TTTCTTTAGA CCCTGTGGTC GATGGGATTT TGATGGACTC GTTTTGCCAG 
CAGCAGGGAT GGGTGCGGGT GTACGATGTG AAGGGTCCCG CCAGTCACCA CTTCTCCTGC 
GGACAGTCGC CTTTCACCGA GCCCTGCGTT TGGGAGAGGA AATACTGCAT CCTGACTGAT 
AGCCAGCTCA TCCTGCTCAA CAAGGAGGAA GAGATGCCGT CAGAGGTGCA TGAAAGCCCC 
TCAGCATCCT CTTCGAAGGG CCGCAGTTTG AGGCGGACAG TCAGTGTGCC TTCTGAAGGA 
CCGTTCCCAG ACTTCCCACC AGAGGGAGCC TCCATGATAG CGGAAGCGTC CGCGGAGCGC 
TCTCCCAGAC GGAGGAGCAT ATCAGGGCTA GGCAGCTCAG AGAAGAACAT TTCCATCGAT 
GGCCCCAACT CATCTCCCTT CAAAGTGCCT GGTTTTTTAA GTAAACGACT GAAAGGATCC 
ATCAAAAGGA CGAAGAGCCA GACTAAGCTG GACCGCAACA CAAGTTTCAG ACTCCCATCG 
CTGCGACCAA CAGAAAATGA CAGGTCACGA GGCCTGCCCA AATTGAAGGA GTCCTGTTCC 
CATGAGTCTT TGCTGAGTCC TGGCAGCGCT GTTGAAGCTC TGGATCTGAG TATGGAGGAG 
GATGTCTACA TCAAACCTTT ACACAGCAGC ATCCTAGGAC AAGATTTCTG CTTTGAGGTG 
GCGTACTCAG GTGGAAGTAA GTGCTTCAGC TGTTCCTCTG CTGCTGAACG GGACAAATGG 
ATGGAGAACC TCAAAAGAAC TGTGCAACCT AATAAGGATA ATTGCCGGCG GGCGGAAAAC 
GTCCTCCGCC TGTGGATCAT TGAGGCAAAA GACCTGCCAC CAAAAAAGAA ATATTTCTGT 
GAGCTGTGTC TGGATGACAT GCTGTACGCT CGTACCACCA GCAAAACCCG CTCCGACTGC 
CTGTTCTGGG GGGAACACTT TGAGTTTTCA GGCCTCCCGT CCATGAAAAG CATCACCATA 
CACATCTACC GGGATGTGGA CAAGAAAAAG AAAAAGGACA AAAACAACTA TGTTGGCCTG 
GTGAACATCC CTGTGCATGG GGTGATGGGT AGGCAGTTTG TGGAGAAGTG GTATCCAGTC 
AGCACTCCCA CCACCAGCAA AGCCAAAGGT GGAGGCCCCT CCATCCGCAT CAAATCCCGC 
TTTCAGACCA TCTCAATTCT GCCCATGGAG CAGTACAAGG AGTTTGCGGA GTTTGTTACC 
AATAACTATA CCATGTTGTG CTCTGTGTTG GAGCCGGTCA TAAGCGTGAA GAACAAAGAG 
GAGATGGCCA GCGCTCTGGT GCACATTCTG CAGAGCACGG GACGGGCAAA GGACTTCCTA 
ACGGATTTGG TGATGTCAGA AGTGGATCGC TGTGCTGACC ATGATGTGCT GATCTTTAGA 
GAAAACACAC TAGCCACCAA AGCCATTGAA GAATACCTCA AACTGGTGGG ACAGAAGTAC 
CTACATGATG CACTAGGGGA GTTTATTAAA GCCCTGTACG AGTCGGATGA AAATTGTGAA 
GTAGACCAAT CAAAGTGCAC CAGCAACGAG CTTCCAGAGC ATCAGAGTAA CCTGAAGATG 
TGCTGCGAAC TGGCCTTCTG CAAGATCATC AACTCTTATT GTGTTTTTCC ACGTGAACTG 
AAGGAGGTCT TTGCATCATG GAAGCAGCAG TGTCACGCTC GAGGGAAGCA GGACATCAGT 
CAGCGTCTTA TCAGCGCATC GCTCTTCCTG CGCTTTCTGT GTCCTGCCAT CATGTCCCCG 
TCACTCTTTG GTCTCATGCA GGAATATCCA GATGACCGTA CTTCTCGAAC ACTCACCCTC 
ATTGCCAAAG TCATTCAGAA TCTTGCTAAC TTTGCCAAAT TCGGGAACAA AGAGGAGTAC 
ATGGCTTTCA TGAATGACTT TCTAGAGCAC GAGTGGGCTG GTATGACCCG TTTTCTGCTT 
GAGATCTCGA ATCTTGAGAC AATCTCCAAC ACCCCGGGCT TTGAGGGCTA CATTGACCTG 
GGCCGGGAGC TGTCAGTGCT TCACTCGCTG CTCTGGGAGG TGGTGTCTCA GCTGGACAAG 
GGTGACAATT CCTTCCTGCA GGCGACGGTG GCCAAACTGG GGCCCCTGCC ACGTATCCTT 
GGCGATATCA CCCGCTCGCT GTCCAGCCCT ACTCCCATCC AGCAGCAGCT CAGGCGCTTC 
CAGGACCACA GCTCTGCTCA TGACATCAGT GGAAGTGTGT CCTCAGGACT GCAGAGAATA 
TTTGAAGACC CAGCAGACAG TGAAATGAGG AGCATCAAAT CACCTGTCCA AGAGCACATG 
GAGGCTTTAG TCAGAGGAAA GCATCCTTTA CTGGGTCAAC AGTCGTCCGC ACACAGCATG 
AGCTTCTCCG ACAAAGAGGA AAGGGACAGT CCGCTCCCGA ATGGACGCAG TATATCTTTA 
ATGGACTTGC AGGACTCTTA CCTTGCTCAG GGGCATCCAG GTCCCAACTC GCTCAATGAA 
GCCTCATCTA GGTTAGGCAG AGTAGGCTCT CAGGCCTCCA TCGGCCCTGT CCCTCCTCCA 
CACCTCCACC AGCCTCCAGT CCACCCGAAA GTTCCACAGC TGAGGGATAA CTTACCCCAG 
AGTGCACCAC AGGTGCGGCG CCCGATCCAC CCATCCCTTA GCCAGCAGCG CAGCCTACAA 
CCGCTGTCTT TCCAAAACCC TGTTTACCAC CTCAGCAACC TGCACGCACA ATCAACACAC 
TCAACCCAGT CCACACACTC TGCCCAGTCC CTGCAGCCTG ACTCTAGCTC AGAAAACCTG 
AGCACAGGGA GCTCTCGGAG CGCCAGTCCT AGTGCCTCAG GTGGCCGGGG AGCAACTCCC 
AGAGCCCGGA TACCCTCCAC AAGCAGCGTG GAGGAGGAAT TTAGCCGAAG AAGCATCCAG 
GGACAGGAGA CGCCGCCTCC AACGGCACGC TGGCACCCTC CCCTGGCAGA CCAGCACTCG 
GGAGCCCAGG TGGTGGCAGT GCCAAGGCAG AGTACCACCG GTACGGCACA TATAGTGAAA 
GTGGAGCAGC AGAGTCGAGG AGTTGGGTTG GTGGCAGCAA ATGCTGGAGC ACGAACCCCA 
AGGTCGCTCC CTCACAGCAC GTCCATCCGC AGCGGCAGCA GCGCCAACAC TGAACCCATA 
CAGCAGAGCG GTGAACGATC AAGACAGCAG TCCACGTGCT CCAAAGATGG TTCAGTACCA 
GGAGGACGAG CCAACAAACA GGTCCAGTCA CCTGTAGAGT CTGTCACCAT GTCCCCAGTG 
GAGCGAACAG CTGCGTGGGT TTTGAATAAT GGCCAGTATG AAGATGAGGA AGAGGAAGGG 
CAGAGTAAAG ACGATGCCAA ACATGTGGAG AAGTATGAAC AGGAAATCTC GAAGCTAAAG 
GAGCGTCTGC GGACGTCTAG TCGGCGGCTG GAGGAGTATG AGAGACGGTT ACTGGCTCAG 
GAGCAGCAGA TGCAGAAGCT GCTCTTAGAA TACAAGACTC GACTGGAGGA CAGTGAGGAC 
CGTCTGCGAA GACAACAGGA GGAAAAAGAC AGCCAGATGA AGAGTATTAT CTGCAGGCTG 
ATGGCCGTGG AGGAAGAGCT GAAGAGAGAC CATGCAGAGA TGCAGGCAGT CATAGATGCC 
AAACAGAAGA TAATCGATGC ACAGGAGAAG AGGATCAGCT CTCTGGATGC TGCTAACTCT 
CGGCTGATGA GCGCTCTGAC TCAGGTGAAG GAGCGCTACA GCATGCACAA CCTCCGGAAC 
GGCCTGTCAC CCACCAACCC CACCAAACTC TCCATCACAG AGAACGGGGA GTTCAAGAAT 
AGCAGCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasal2 ( XM_001332652.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001332688.5 Danio rerio RAS protein activator like 2 (rasal2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa07239
Clone ID Related Accession (Same CDS sequence)	XM_001332652.8 , XM_001332652.7
Accession Version	XM_001332652.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras GTPase-activating protein nGAP isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001332652.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ACTCAGTGAT ACCAAACCAA CACAAACGAA CGGAAAACAT TGTGATTGAG 
CCTCTTCTGC CACGCGCTTG GAAACCCAGC GGACTGGAGT CTTTACCTGG ACGTTACCGT 
GGAGGTGTAT CGAGCATGGA GACGGATTCG GTAACCGGTG GCGACGTGGA CTCGGTTCAG 
GGAGGCATGC TTTCTTTAGA CCCTGTGGTC GATGGGATTT TGATGGACTC GTTTTGCCAG 
CAGCAGGGAT GGGTGCGGGT GTACGATGTG AAGGGTCCCG CCAGTCACCA CTTCTCCTGC 
GGACAGTCGC CTTTCACCGA GCCCTGCGTT TGGGAGAGGA AATACTGCAT CCTGACTGAT 
AGCCAGCTCA TCCTGCTCAA CAAGGAGGAA GAGATGCCGT CAGAGGTGCA TGAAAGCCCC 
TCAGCATCCT CTTCGAAGGG CCGCAGTTTG AGGCGGACAG TCAGTGTGCC TTCTGAAGGA 
CCGTTCCCAG ACTTCCCACC AGAGGGAGCC TCCATGATAG CGGAAGCGTC CGCGGAGCGC 
TCTCCCAGAC GGAGGAGCAT ATCAGGGCTA GGCAGCTCAG AGAAGAACAT TTCCATCGAT 
GGCCCCAACT CATCTCCCTT CAAAGTGCCT GGTTTTTTAA GTAAACGACT GAAAGGATCC 
ATCAAAAGGA CGAAGAGCCA GACTAAGCTG GACCGCAACA CAAGTTTCAG ACTCCCATCG 
CTGCGACCAA CAGAAAATGA CAGGTCACGA GGCCTGCCCA AATTGAAGGA GTCCTGTTCC 
CATGAGTCTT TGCTGAGTCC TGGCAGCGCT GTTGAAGCTC TGGATCTGAG TATGGAGGAG 
GATGTCTACA TCAAACCTTT ACACAGCAGC ATCCTAGGAC AAGATTTCTG CTTTGAGGTG 
GCGTACTCAG GTGGAAGTAA GTGCTTCAGC TGTTCCTCTG CTGCTGAACG GGACAAATGG 
ATGGAGAACC TCAAAAGAAC TGTGCAACCT AATAAGGATA ATTGCCGGCG GGCGGAAAAC 
GTCCTCCGCC TGTGGATCAT TGAGGCAAAA GACCTGCCAC CAAAAAAGAA ATATTTCTGT 
GAGCTGTGTC TGGATGACAT GCTGTACGCT CGTACCACCA GCAAAACCCG CTCCGACTGC 
CTGTTCTGGG GGGAACACTT TGAGTTTTCA GGCCTCCCGT CCATGAAAAG CATCACCATA 
CACATCTACC GGGATGTGGA CAAGAAAAAG AAAAAGGACA AAAACAACTA TGTTGGCCTG 
GTGAACATCC CTGTGCATGG GGTGATGGGT AGGCAGTTTG TGGAGAAGTG GTATCCAGTC 
AGCACTCCCA CCACCAGCAA AGCCAAAGGT GGAGGCCCCT CCATCCGCAT CAAATCCCGC 
TTTCAGACCA TCTCAATTCT GCCCATGGAG CAGTACAAGG AGTTTGCGGA GTTTGTTACC 
AATAACTATA CCATGTTGTG CTCTGTGTTG GAGCCGGTCA TAAGCGTGAA GAACAAAGAG 
GAGATGGCCA GCGCTCTGGT GCACATTCTG CAGAGCACGG GACGGGCAAA GGACTTCCTA 
ACGGATTTGG TGATGTCAGA AGTGGATCGC TGTGCTGACC ATGATGTGCT GATCTTTAGA 
GAAAACACAC TAGCCACCAA AGCCATTGAA GAATACCTCA AACTGGTGGG ACAGAAGTAC 
CTACATGATG CACTAGGGGA GTTTATTAAA GCCCTGTACG AGTCGGATGA AAATTGTGAA 
GTAGACCAAT CAAAGTGCAC CAGCAACGAG CTTCCAGAGC ATCAGAGTAA CCTGAAGATG 
TGCTGCGAAC TGGCCTTCTG CAAGATCATC AACTCTTATT GTGTTTTTCC ACGTGAACTG 
AAGGAGGTCT TTGCATCATG GAAGCAGCAG TGTCACGCTC GAGGGAAGCA GGACATCAGT 
CAGCGTCTTA TCAGCGCATC GCTCTTCCTG CGCTTTCTGT GTCCTGCCAT CATGTCCCCG 
TCACTCTTTG GTCTCATGCA GGAATATCCA GATGACCGTA CTTCTCGAAC ACTCACCCTC 
ATTGCCAAAG TCATTCAGAA TCTTGCTAAC TTTGCCAAAT TCGGGAACAA AGAGGAGTAC 
ATGGCTTTCA TGAATGACTT TCTAGAGCAC GAGTGGGCTG GTATGACCCG TTTTCTGCTT 
GAGATCTCGA ATCTTGAGAC AATCTCCAAC ACCCCGGGCT TTGAGGGCTA CATTGACCTG 
GGCCGGGAGC TGTCAGTGCT TCACTCGCTG CTCTGGGAGG TGGTGTCTCA GCTGGACAAG 
GGTGACAATT CCTTCCTGCA GGCGACGGTG GCCAAACTGG GGCCCCTGCC ACGTATCCTT 
GGCGATATCA CCCGCTCGCT GTCCAGCCCT ACTCCCATCC AGCAGCAGCT CAGGCGCTTC 
CAGGACCACA GCTCTGCTCA TGACATCAGT GGAAGTGTGT CCTCAGGACT GCAGAGAATA 
TTTGAAGACC CAGCAGACAG TGAAATGAGG AGCATCAAAT CACCTGTCCA AGAGCACATG 
GAGGCTTTAG TCAGAGGAAA GCATCCTTTA CTGGGTCAAC AGTCGTCCGC ACACAGCATG 
AGCTTCTCCG ACAAAGAGGA AAGGGACAGT CCGCTCCCGA ATGGACGCAG TATATCTTTA 
ATGGACTTGC AGGACTCTTA CCTTGCTCAG GGGCATCCAG GTCCCAACTC GCTCAATGAA 
GCCTCATCTA GGTTAGGCAG AGTAGGCTCT CAGGCCTCCA TCGGCCCTGT CCCTCCTCCA 
CACCTCCACC AGCCTCCAGT CCACCCGAAA GTTCCACAGC TGAGGGATAA CTTACCCCAG 
AGTGCACCAC AGGTGCGGCG CCCGATCCAC CCATCCCTTA GCCAGCAGCG CAGCCTACAA 
CCGCTGTCTT TCCAAAACCC TGTTTACCAC CTCAGCAACC TGCACGCACA ATCAACACAC 
TCAACCCAGT CCACACACTC TGCCCAGTCC CTGCAGCCTG ACTCTAGCTC AGAAAACCTG 
AGCACAGGGA GCTCTCGGAG CGCCAGTCCT AGTGCCTCAG GTGGCCGGGG AGCAACTCCC 
AGAGCCCGGA TACCCTCCAC AAGCAGCGTG GAGGAGGAAT TTAGCCGAAG AAGCATCCAG 
GGACAGGAGA CGCCGCCTCC AACGGCACGC TGGCACCCTC CCCTGGCAGA CCAGCACTCG 
GGAGCCCAGG TGGTGGCAGT GCCAAGGCAG AGTACCACCG GTACGGCACA TATAGTGAAA 
GTGGAGCAGC AGAGTCGAGG AGTTGGGTTG GTGGCAGCAA ATGCTGGAGC ACGAACCCCA 
AGGTCGCTCC CTCACAGCAC GTCCATCCGC AGCGGCAGCA GCGCCAACAC TGAACCCATA 
CAGCAGAGCG GTGAACGATC AAGACAGCAG TCCACGTGCT CCAAAGATGG TTCAGTACCA 
GGAGGACGAG CCAACAAACA GGTCCAGTCA CCTGTAGAGT CTGTCACCAT GTCCCCAGTG 
GAGCGAACAG CTGCGTGGGT TTTGAATAAT GGCCAGTATG AAGATGAGGA AGAGGAAGGG 
CAGAGTAAAG ACGATGCCAA ACATGTGGAG AAGTATGAAC AGGAAATCTC GAAGCTAAAG 
GAGCGTCTGC GGACGTCTAG TCGGCGGCTG GAGGAGTATG AGAGACGGTT ACTGGCTCAG 
GAGCAGCAGA TGCAGAAGCT GCTCTTAGAA TACAAGACTC GACTGGAGGA CAGTGAGGAC 
CGTCTGCGAA GACAACAGGA GGAAAAAGAC AGCCAGATGA AGAGTATTAT CTGCAGGCTG 
ATGGCCGTGG AGGAAGAGCT GAAGAGAGAC CATGCAGAGA TGCAGGCAGT CATAGATGCC 
AAACAGAAGA TAATCGATGC ACAGGAGAAG AGGATCAGCT CTCTGGATGC TGCTAACTCT 
CGGCTGATGA GCGCTCTGAC TCAGGTGAAG GAGCGCTACA GCATGCACAA CCTCCGGAAC 
GGCCTGTCAC CCACCAACCC CACCAAACTC TCCATCACAG AGAACGGGGA GTTCAAGAAT 
AGCAGCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001332688.5
CDS	619..4650
Translation	MVVNSVIPNQHKRTENIVIEPLLPRAWKPSGLESLPGRYRGGVSSMETDSVTGGDVDSVQGGMLSLDPVVDGILMDSFCQQQGWVRVYDVKGPASHHFSCGQSPFTEPCVWERKYCILTDSQLILLNKEEEMPSEVHESPSASSSKGRSLRRTVSVPSEGPFPDFPPEGASMIAEASAERSPRRRSISGLGSSEKNISIDGPNSSPFKVPGFLSKRLKGSIKRTKSQTKLDRNTSFRLPSLRPTENDRSRGLPKLKESCSHESLLSPGSAVEALDLSMEEDVYIKPLHSSILGQDFCFEVAYSGGSKCFSCSSAAERDKWMENLKRTVQPNKDNCRRAENVLRLWIIEAKDLPPKKKYFCELCLDDMLYARTTSKTRSDCLFWGEHFEFSGLPSMKSITIHIYRDVDKKKKKDKNNYVGLVNIPVHGVMGRQFVEKWYPVSTPTTSKAKGGGPSIRIKSRFQTISILPMEQYKEFAEFVTNNYTMLCSVLEPVISVKNKEEMASALVHILQSTGRAKDFLTDLVMSEVDRCADHDVLIFRENTLATKAIEEYLKLVGQKYLHDALGEFIKALYESDENCEVDQSKCTSNELPEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCHARGKQDISQRLISASLFLRFLCPAIMSPSLFGLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWAGMTRFLLEISNLETISNTPGFEGYIDLGRELSVLHSLLWEVVSQLDKGDNSFLQATVAKLGPLPRILGDITRSLSSPTPIQQQLRRFQDHSSAHDISGSVSSGLQRIFEDPADSEMRSIKSPVQEHMEALVRGKHPLLGQQSSAHSMSFSDKEERDSPLPNGRSISLMDLQDSYLAQGHPGPNSLNEASSRLGRVGSQASIGPVPPPHLHQPPVHPKVPQLRDNLPQSAPQVRRPIHPSLSQQRSLQPLSFQNPVYHLSNLHAQSTHSTQSTHSAQSLQPDSSSENLSTGSSRSASPSASGGRGATPRARIPSTSSVEEEFSRRSIQGQETPPPTARWHPPLADQHSGAQVVAVPRQSTTGTAHIVKVEQQSRGVGLVAANAGARTPRSLPHSTSIRSGSSANTEPIQQSGERSRQQSTCSKDGSVPGGRANKQVQSPVESVTMSPVERTAAWVLNNGQYEDEEEEGQSKDDAKHVEKYEQEISKLKERLRTSSRRLEEYERRLLAQEQQMQKLLLEYKTRLEDSEDRLRRQQEEKDSQMKSIICRLMAVEEELKRDHAEMQAVIDAKQKIIDAQEKRISSLDAANSRLMSALTQVKERYSMHNLRNGLSPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_001332652.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio RAS protein activator like 2 (rasal2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001332652.7

ORF Insert Sequence:

ATGGTTGTAA ACTCAGTGAT ACCAAACCAA CACAAACGAA CGGAAAACAT TGTGATTGAG 
CCTCTTCTGC CACGCGCTTG GAAACCCAGC GGACTGGAGT CTTTACCTGG ACGTTACCGT 
GGAGGTGTAT CGAGCATGGA GACGGATTCG GTAACCGGTG GCGACGTGGA CTCGGTTCAG 
GGAGGCATGC TTTCTTTAGA CCCTGTGGTC GATGGGATTT TGATGGACTC GTTTTGCCAG 
CAGCAGGGAT GGGTGCGGGT GTACGATGTG AAGGGTCCCG CCAGTCACCA CTTCTCCTGC 
GGACAGTCGC CTTTCACCGA GCCCTGCGTT TGGGAGAGGA AATACTGCAT CCTGACTGAT 
AGCCAGCTCA TCCTGCTCAA CAAGGAGGAA GAGATGCCGT CAGAGGTGCA TGAAAGCCCC 
TCAGCATCCT CTTCGAAGGG CCGCAGTTTG AGGCGGACAG TCAGTGTGCC TTCTGAAGGA 
CCGTTCCCAG ACTTCCCACC AGAGGGAGCC TCCATGATAG CGGAAGCGTC CGCGGAGCGC 
TCTCCCAGAC GGAGGAGCAT ATCAGGGCTA GGCAGCTCAG AGAAGAACAT TTCCATCGAT 
GGCCCCAACT CATCTCCCTT CAAAGTGCCT GGTTTTTTAA GTAAACGACT GAAAGGATCC 
ATCAAAAGGA CGAAGAGCCA GACTAAGCTG GACCGCAACA CAAGTTTCAG ACTCCCATCG 
CTGCGACCAA CAGAAAATGA CAGGTCACGA GGCCTGCCCA AATTGAAGGA GTCCTGTTCC 
CATGAGTCTT TGCTGAGTCC TGGCAGCGCT GTTGAAGCTC TGGATCTGAG TATGGAGGAG 
GATGTCTACA TCAAACCTTT ACACAGCAGC ATCCTAGGAC AAGATTTCTG CTTTGAGGTG 
GCGTACTCAG GTGGAAGTAA GTGCTTCAGC TGTTCCTCTG CTGCTGAACG GGACAAATGG 
ATGGAGAACC TCAAAAGAAC TGTGCAACCT AATAAGGATA ATTGCCGGCG GGCGGAAAAC 
GTCCTCCGCC TGTGGATCAT TGAGGCAAAA GACCTGCCAC CAAAAAAGAA ATATTTCTGT 
GAGCTGTGTC TGGATGACAT GCTGTACGCT CGTACCACCA GCAAAACCCG CTCCGACTGC 
CTGTTCTGGG GGGAACACTT TGAGTTTTCA GGCCTCCCGT CCATGAAAAG CATCACCATA 
CACATCTACC GGGATGTGGA CAAGAAAAAG AAAAAGGACA AAAACAACTA TGTTGGCCTG 
GTGAACATCC CTGTGCATGG GGTGATGGGT AGGCAGTTTG TGGAGAAGTG GTATCCAGTC 
AGCACTCCCA CCACCAGCAA AGCCAAAGGT GGAGGCCCCT CCATCCGCAT CAAATCCCGC 
TTTCAGACCA TCTCAATTCT GCCCATGGAG CAGTACAAGG AGTTTGCGGA GTTTGTTACC 
AATAACTATA CCATGTTGTG CTCTGTGTTG GAGCCGGTCA TAAGCGTGAA GAACAAAGAG 
GAGATGGCCA GCGCTCTGGT GCACATTCTG CAGAGCACGG GACGGGCAAA GGACTTCCTA 
ACGGATTTGG TGATGTCAGA AGTGGATCGC TGTGCTGACC ATGATGTGCT GATCTTTAGA 
GAAAACACAC TAGCCACCAA AGCCATTGAA GAATACCTCA AACTGGTGGG ACAGAAGTAC 
CTACATGATG CACTAGGGGA GTTTATTAAA GCCCTGTACG AGTCGGATGA AAATTGTGAA 
GTAGACCAAT CAAAGTGCAC CAGCAACGAG CTTCCAGAGC ATCAGAGTAA CCTGAAGATG 
TGCTGCGAAC TGGCCTTCTG CAAGATCATC AACTCTTATT GTGTTTTTCC ACGTGAACTG 
AAGGAGGTCT TTGCATCATG GAAGCAGCAG TGTCACGCTC GAGGGAAGCA GGACATCAGT 
CAGCGTCTTA TCAGCGCATC GCTCTTCCTG CGCTTTCTGT GTCCTGCCAT CATGTCCCCG 
TCACTCTTTG GTCTCATGCA GGAATATCCA GATGACCGTA CTTCTCGAAC ACTCACCCTC 
ATTGCCAAAG TCATTCAGAA TCTTGCTAAC TTTGCCAAAT TCGGGAACAA AGAGGAGTAC 
ATGGCTTTCA TGAATGACTT TCTAGAGCAC GAGTGGGCTG GTATGACCCG TTTTCTGCTT 
GAGATCTCGA ATCTTGAGAC AATCTCCAAC ACCCCGGGCT TTGAGGGCTA CATTGACCTG 
GGCCGGGAGC TGTCAGTGCT TCACTCGCTG CTCTGGGAGG TGGTGTCTCA GCTGGACAAG 
GGTGACAATT CCTTCCTGCA GGCGACGGTG GCCAAACTGG GGCCCCTGCC ACGTATCCTT 
GGCGATATCA CCCGCTCGCT GTCCAGCCCT ACTCCCATCC AGCAGCAGCT CAGGCGCTTC 
CAGGACCACA GCTCTGCTCA TGACATCAGT GGAAGTGTGT CCTCAGGACT GCAGAGAATA 
TTTGAAGACC CAGCAGACAG TGAAATGAGG AGCATCAAAT CACCTGTCCA AGAGCACATG 
GAGGCTTTAG TCAGAGGAAA GCATCCTTTA CTGGGTCAAC AGTCGTCCGC ACACAGCATG 
AGCTTCTCCG ACAAAGAGGA AAGGGACAGT CCGCTCCCGA ATGGACGCAG TATATCTTTA 
ATGGACTTGC AGGACTCTTA CCTTGCTCAG GGGCATCCAG GTCCCAACTC GCTCAATGAA 
GCCTCATCTA GGTTAGGCAG AGTAGGCTCT CAGGCCTCCA TCGGCCCTGT CCCTCCTCCA 
CACCTCCACC AGCCTCCAGT CCACCCGAAA GTTCCACAGC TGAGGGATAA CTTACCCCAG 
AGTGCACCAC AGGTGCGGCG CCCGATCCAC CCATCCCTTA GCCAGCAGCG CAGCCTACAA 
CCGCTGTCTT TCCAAAACCC TGTTTACCAC CTCAGCAACC TGCACGCACA ATCAACACAC 
TCAACCCAGT CCACACACTC TGCCCAGTCC CTGCAGCCTG ACTCTAGCTC AGAAAACCTG 
AGCACAGGGA GCTCTCGGAG CGCCAGTCCT AGTGCCTCAG GTGGCCGGGG AGCAACTCCC 
AGAGCCCGGA TACCCTCCAC AAGCAGCGTG GAGGAGGAAT TTAGCCGAAG AAGCATCCAG 
GGACAGGAGA CGCCGCCTCC AACGGCACGC TGGCACCCTC CCCTGGCAGA CCAGCACTCG 
GGAGCCCAGG TGGTGGCAGT GCCAAGGCAG AGTACCACCG GTACGGCACA TATAGTGAAA 
GTGGAGCAGC AGAGTCGAGG AGTTGGGTTG GTGGCAGCAA ATGCTGGAGC ACGAACCCCA 
AGGTCGCTCC CTCACAGCAC GTCCATCCGC AGCGGCAGCA GCGCCAACAC TGAACCCATA 
CAGCAGAGCG GTGAACGATC AAGACAGCAG TCCACGTGCT CCAAAGATGG TTCAGTACCA 
GGAGGACGAG CCAACAAACA GGTCCAGTCA CCTGTAGAGT CTGTCACCAT GTCCCCAGTG 
GAGCGAACAG CTGCGTGGGT TTTGAATAAT GGCCAGTATG AAGATGAGGA AGAGGAAGGG 
CAGAGTAAAG ACGATGCCAA ACATGTGGAG AAGTATGAAC AGGAAATCTC GAAGCTAAAG 
GAGCGTCTGC GGACGTCTAG TCGGCGGCTG GAGGAGTATG AGAGACGGTT ACTGGCTCAG 
GAGCAGCAGA TGCAGAAGCT GCTCTTAGAA TACAAGACTC GACTGGAGGA CAGTGAGGAC 
CGTCTGCGAA GACAACAGGA GGAAAAAGAC AGCCAGATGA AGAGTATTAT CTGCAGGCTG 
ATGGCCGTGG AGGAAGAGCT GAAGAGAGAC CATGCAGAGA TGCAGGCAGT CATAGATGCC 
AAACAGAAGA TAATCGATGC ACAGGAGAAG AGGATCAGCT CTCTGGATGC TGCTAACTCT 
CGGCTGATGA GCGCTCTGAC TCAGGTGAAG GAGCGCTACA GCATGCACAA CCTCCGGAAC 
GGCCTGTCAC CCACCAACCC CACCAAACTC TCCATCACAG AGAACGGGGA GTTCAAGAAT 
AGCAGCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	rasal2
Entrez Gene ID	793730
Full Name	RAS protein activator like 2
Synonyms	fc75a03,si:ch211-198i20.2,wu:fc75a03
General protein information	Preferred Names RAS protein activator like 2 Names ras GTPase-activating protein nGAP
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Danio rerio (zebrafish)	rasal2	XP_001332688.5
Xenopus tropicalis (tropical clawed frog)	rasal2	XP_004914087.1
Homo sapiens (human)	RASAL2	NP_733793.2
Gallus gallus (chicken)	RASAL2	XP_004943302.1
Canis lupus familiaris (dog)	RASAL2	XP_005622535.1
Bos taurus (cattle)	RASAL2	XP_005217296.1
Pan troglodytes (chimpanzee)	RASAL2	XP_003949696.1
Mus musculus (house mouse)	Rasal2	NP_808312.3
	RASAL2	XP_002802086.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

rasal2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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