b4galt5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	b4galt5
Entrez Gene ID	724080
Full Name	UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5
General protein information	Preferred Names UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 Names beta-1,4-galactosyltransferase 5
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001045332.1	NP_001038797.1	beta-1,4-galactosyltransferase 5

Pathways from BioSystems

Homologies

Homo sapiens (human)	B4GALT5	NP_004767.1
Pan troglodytes (chimpanzee)	B4GALT5	XP_001167173.1
Caenorhabditis elegans (roundworm)	W02B12.11	NP_496449.1
	B4GALT5	XP_001103384.2
Danio rerio (zebrafish)	b4galt5	NP_001038797.1
Canis lupus familiaris (dog)	B4GALT5	XP_543044.4
Bos taurus (cattle)	B4GALT5	XP_002692400.2
Mus musculus (house mouse)	B4galt5	NP_062809.2
Xenopus tropicalis (tropical clawed frog)	b4galt6	NP_001016385.1
Gallus gallus (chicken)	B4GALT5	XP_417519.4

Gene Ontology
Bibliography

Related articles in PubMed

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Briolat V, Jouneau L, Carvalho R, Palha N, Langevin C, Herbomel P, Schwartz O, Spaink HP, Levraud JP, Boudinot P
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)

A beta1,4-galactosyltransferase is required for Bmp2-dependent patterning of the dorsoventral axis during zebrafish embryogenesis.
Machingo QJ, Fritz A, Shur BD
Development (Cambridge, England)133(11)2233-41(2006 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

beta4GalT5 is a previously unidentified zebrafish galactosyltransferase that is essential for proper patterning of the dorsoventral axis by regulating bone morphogenetic protein 2 signaling
Title: A beta1,4-galactosyltransferase is required for Bmp2-dependent patterning of the dorsoventral axis during zebrafish embryogenesis.

The following b4galt5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the b4galt5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33609	NM_001045332.1 Latest version!	Danio rerio UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 (b4galt5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

b4galt5 ( NM_001045332.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038797.1 Danio rerio UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 (b4galt5), mRNA.

Price & Availability↑

CloneID	ODa33609
Clone ID Related Accession (Same CDS sequence)	NM_001045332.1
Accession Version	NM_001045332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Danio rerio(zebrafish)
Product	beta-1,4-galactosyltransferase 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ104219.1. ##Evidence-Data-START## Transcript exon combination :: DQ104219.1, BC163615.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA4476793 [ECO:0000348] ##Evidence-Data-END##

ATGCCGACAC ATCTGAGATT TCGGAGAAGG TCATTCCTCG GACTGTTATT TCTCTTCTCG 
CTCTCCACAT CTGCACTGTA CTTCATCTAC TCGGCTCCTG GAATAGTTAA TGAGTACCTG 
TTCATGGTTC AGGCCCGTGG AATCCAGATC CGTGAAAATG TGAGGAATAT GGGGGCTCAG 
GTTCTGGAGC AGGTGGTCCG CAGTGCTTAC AGCATCAACG GCACAGAGTA CACCTATGAG 
TTTAACTTCA GCGAGACAGA CGCGTCCCCC ACTCCTTTTC TCCCGGAGGG CTTCACGTAT 
AAGCCTGAGC AGGTCTGCCC AGAGAAGCTG CCCTCCATGA AGGGCCGGCT GAAGGTGAAC 
ATGAGTGAGA TCGCTCTAGA CGAGGTGGAG AAACTGCTGA AGCTAAATGA TCCGGGTCTG 
TCAGTCGGAG GCCACTGGAA ACCCCATGAC TGTCGCCCAC GCTGGAAGGT GGCCATTTTG 
GTTCCTTTCC GAAATCGCCA TGAGCACCTT CCAATACTCT TCCGGCATCT GATCCCAGCG 
CTGCAGAGAC AGAGACTGCA GTTCGGCTTC TACGTCATTG AACAGGCAGG AAATGAACCC 
TTCAACCGTG CCATGCTGTT TAATGTCGGA TTCAAAGAGG CCATGAAGGA CTTGAACTGG 
GATTGCGTGA TATTCCACGA TGTGGACCAC ATTCTGGAAA ATGACCGCAA CTATTACGGA 
TGTGGTGAAA TGCCCAGACA TTTTGCGGTC AAACTCAACA AGTACTCATA CATGCTTCCA 
TATGAGGAGT TTTTTGGTGG AGTGAGCGGA CTCACCGTCA AGCAATTCAA AAGGATAAAT 
GGCTTTCCCA ATGCATTCTG GGGCTGGGGA GGAGAAGACG ATGACCTTTG GAACAGAGTT 
CAATTTGCTG GATACAAGGT GAGCCGTCCT CATGGAGAGC TGGGCCGCTA CATGTCCATC 
CCGCACCACC ACCGAGGAGA AGTGCAGTTT CTGGGCAGAT ACAAGTTGCT CCGTAGGTCG 
AAGGAGAGGC AGAGTCTGGA CGGCCTGAAT AACTTGAATT ACTCCCCTCT AGTGTCCCGG 
AGGAGTCTTT ACACCAACGT GTCAGTGACG CTCAGTCGAG ATCTGGCTCC TGTGGCCGAT 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038797.1
CDS	249..1394
Translation	MPTHLRFRRRSFLGLLFLFSLSTSALYFIYSAPGIVNEYLFMVQARGIQIRENVRNMGAQVLEQVVRSAYSINGTEYTYEFNFSETDASPTPFLPEGFTYKPEQVCPEKLPSMKGRLKVNMSEIALDEVEKLLKLNDPGLSVGGHWKPHDCRPRWKVAILVPFRNRHEHLPILFRHLIPALQRQRLQFGFYVIEQAGNEPFNRAMLFNVGFKEAMKDLNWDCVIFHDVDHILENDRNYYGCGEMPRHFAVKLNKYSYMLPYEEFFGGVSGLTVKQFKRINGFPNAFWGWGGEDDDLWNRVQFAGYKVSRPHGELGRYMSIPHHHRGEVQFLGRYKLLRRSKERQSLDGLNNLNYSPLVSRRSLYTNVSVTLSRDLAPVADY

Target ORF information:

RefSeq Version	NM_001045332.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 (b4galt5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045332.1

ORF Insert Sequence:

ATGCCGACAC ATCTGAGATT TCGGAGAAGG TCATTCCTCG GACTGTTATT TCTCTTCTCG 
CTCTCCACAT CTGCACTGTA CTTCATCTAC TCGGCTCCTG GAATAGTTAA TGAGTACCTG 
TTCATGGTTC AGGCCCGTGG AATCCAGATC CGTGAAAATG TGAGGAATAT GGGGGCTCAG 
GTTCTGGAGC AGGTGGTCCG CAGTGCTTAC AGCATCAACG GCACAGAGTA CACCTATGAG 
TTTAACTTCA GCGAGACAGA CGCGTCCCCC ACTCCTTTTC TCCCGGAGGG CTTCACGTAT 
AAGCCTGAGC AGGTCTGCCC AGAGAAGCTG CCCTCCATGA AGGGCCGGCT GAAGGTGAAC 
ATGAGTGAGA TCGCTCTAGA CGAGGTGGAG AAACTGCTGA AGCTAAATGA TCCGGGTCTG 
TCAGTCGGAG GCCACTGGAA ACCCCATGAC TGTCGCCCAC GCTGGAAGGT GGCCATTTTG 
GTTCCTTTCC GAAATCGCCA TGAGCACCTT CCAATACTCT TCCGGCATCT GATCCCAGCG 
CTGCAGAGAC AGAGACTGCA GTTCGGCTTC TACGTCATTG AACAGGCAGG AAATGAACCC 
TTCAACCGTG CCATGCTGTT TAATGTCGGA TTCAAAGAGG CCATGAAGGA CTTGAACTGG 
GATTGCGTGA TATTCCACGA TGTGGACCAC ATTCTGGAAA ATGACCGCAA CTATTACGGA 
TGTGGTGAAA TGCCCAGACA TTTTGCGGTC AAACTCAACA AGTACTCATA CATGCTTCCA 
TATGAGGAGT TTTTTGGTGG AGTGAGCGGA CTCACCGTCA AGCAATTCAA AAGGATAAAT 
GGCTTTCCCA ATGCATTCTG GGGCTGGGGA GGAGAAGACG ATGACCTTTG GAACAGAGTT 
CAATTTGCTG GATACAAGGT GAGCCGTCCT CATGGAGAGC TGGGCCGCTA CATGTCCATC 
CCGCACCACC ACCGAGGAGA AGTGCAGTTT CTGGGCAGAT ACAAGTTGCT CCGTAGGTCG 
AAGGAGAGGC AGAGTCTGGA CGGCCTGAAT AACTTGAATT ACTCCCCTCT AGTGTCCCGG 
AGGAGTCTTT ACACCAACGT GTCAGTGACG CTCAGTCGAG ATCTGGCTCC TGTGGCCGAT 
TACTGA