fech cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	fech
Entrez Gene ID	58215
Full Name	ferrochelatase
Synonyms	fch,zgc:109851
General protein information	Preferred Names ferrochelatase Names ferrochelatase, mitochondrial dracula drc frx
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021468899.1	XP_021324574.1	ferrochelatase, mitochondrial isoform X1
NM_131631.2	NP_571706.1	ferrochelatase, mitochondrial

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	FECH	NP_989527.1
Kluyveromyces lactis	KLLA0F20141g	XP_455984.1
Eremothecium gossypii	AGOS_AGR272W	NP_986938.1
Schizosaccharomyces pombe (fission yeast)	hem15	NP_587720.1
Xenopus tropicalis (tropical clawed frog)	fech	XP_002934473.1
	FECH	XP_002800996.1
Canis lupus familiaris (dog)	FECH	XP_852936.1
Saccharomyces cerevisiae (baker's yeast)	HEM15	NP_014819.3
Neurospora crassa	NCU08291	XP_962504.1
Oryza sativa (rice)	Os05g0361200	NP_001055309.1
Homo sapiens (human)	FECH	NP_000131.2
Pan troglodytes (chimpanzee)	FECH	NP_001033101.1
Mus musculus (house mouse)	Fech	NP_032024.2
Rattus norvegicus (Norway rat)	Fech	NP_001101904.1
Danio rerio (zebrafish)	fech	NP_571706.1
Drosophila melanogaster (fruit fly)	ferrochelatase	NP_524613.1
Arabidopsis thaliana (thale cress)	FC2	NP_001189639.1
Bos taurus (cattle)	FECH	NP_776479.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003719	XP_310249.3
Magnaporthe oryzae (rice blast fungus)	MGG_15723	XP_003714486.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Edwardsiella tarda-Induced Inhibition of Apoptosis: A Strategy for Intracellular Survival.
Zhou ZJ, Sun L
Frontiers in cellular and infection microbiology676(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following fech gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fech cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37290	NM_131631.2 Latest version!	Danio rerio ferrochelatase (fech), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa150941	XM_021468899.1 Latest version!	Danio rerio ferrochelatase (fech), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fech ( NM_131631.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571706.1 Danio rerio ferrochelatase (fech), mRNA.

Price & Availability↑

CloneID	ODa37290
Clone ID Related Accession (Same CDS sequence)	NM_131631.2
Accession Version	NM_131631.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-12
Organism	Danio rerio(zebrafish)
Product	ferrochelatase, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF250368.1. On Aug 30, 2012 this sequence version replaced NM_131631.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF250368.1, BC092711.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA3505393 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGTTT TAGGAGGCGC GTGCCGGCTC GTGCAGCTGG TCAGATGTGG TTCTCCTGTG 
GGTTTGTGTT TGTCTTCATC TCTCAGACGA CAGTCGACGG CCACAGCAGC GGCGTTCAAC 
ACCACAGCCA CTCCAGAGAC TAAAGAGAGC AGAAAACCCA AAACAGGCAT TCTGATGCTG 
AATATGGGCG GTCCAGAGAA GCTGGAGGAT GTTCATGATT TCCTGCTGCG CCTCTTCATG 
GATACAGACT TCATGCAGCT GCCCGTGCAG AATAAACTCG GGCCATTCAT TGCCAAAAGA 
CGCACCCCAA AGATCCAGGA GCAGTACAGT AAGATTGGCG GAGGGTCTCC CATTAAAGCC 
TGGACCACCA TGCAGGGGGA AGGAATGGTG AAACTGCTGG ACGAGATGTG TCCGGACACG 
GCTCCTCATA AGTTTTACAT TGGTTTCCGG TACGTTCATC CGCTGACAGA GGAGGCCATA 
GAGTTGATGG AGAAAGATGG AGTGGAGAGA GCAGTGGCCT TCACACAGTA CCCACAGTAC 
AGCTGCTCCA CCACCGGCAG CAGTCTGAAC GCCATCTACA GATACTACAG TAATCGCGCC 
GACAGACCGA AGATGCGCTG GAGTGTGATT GACAGGTGGC CGACACACCC GCTACTGATA 
GAGTGTTTCG CGGAGCATGT TCGTAATGAG CTGGACAAGT TTCCTGTAGA GAAGAGAGAT 
GATGTAGTGA TTCTGTTCTC TGCGCACTCT CTGCCCCTAT CTGTGGTGAA CCGGGGTGAT 
CCATACCCAC AGGAAGTGGG CGCTACAGTT CAGAGAGTGA TGGACCGACT TGGCCACTGT 
AACCCATACA GACTCGTCTG GCAGTCAAAG GTTGGGCCGA TGGCGTGGTT GGGGCCACAG 
ACAGATGAGG TCATCAAGGG TCTCTGTCAG CGAGGCAAGA GGAACCTGCT GCTGGTGCCC 
ATCGCCTTCA CCAGCGACCA CATCGAGACG CTGCACGAGC TGGACATCGA GTACTCACAG 
GTCCTCGGAG AAGAAGTTGG AGTGGAAAAC ATCAGAAGAG CAGAATCTCT GAATGGAAAT 
CCACTGTTCT TCAGGGCTCT GGCTGATCTG GTTCAGTCTC ACCTGCAGTC TAATGAATCA 
TGCTCTCGTC AGCTGACCCT GCGCTGTCCG CTGTGTGTGA ACCCAACCTG TGCTCAAACC 
AAAGCCTTCT TCTCCAGCCA GAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571706.1
CDS	69..1298
Translation	MAVLGGACRLVQLVRCGSPVGLCLSSSLRRQSTATAAAFNTTATPETKESRKPKTGILMLNMGGPEKLEDVHDFLLRLFMDTDFMQLPVQNKLGPFIAKRRTPKIQEQYSKIGGGSPIKAWTTMQGEGMVKLLDEMCPDTAPHKFYIGFRYVHPLTEEAIELMEKDGVERAVAFTQYPQYSCSTTGSSLNAIYRYYSNRADRPKMRWSVIDRWPTHPLLIECFAEHVRNELDKFPVEKRDDVVILFSAHSLPLSVVNRGDPYPQEVGATVQRVMDRLGHCNPYRLVWQSKVGPMAWLGPQTDEVIKGLCQRGKRNLLLVPIAFTSDHIETLHELDIEYSQVLGEEVGVENIRRAESLNGNPLFFRALADLVQSHLQSNESCSRQLTLRCPLCVNPTCAQTKAFFSSQKL

Target ORF information:

RefSeq Version	NM_131631.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ferrochelatase (fech), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131631.2

ORF Insert Sequence:

ATGGCGGTTT TAGGAGGCGC GTGCCGGCTC GTGCAGCTGG TCAGATGTGG TTCTCCTGTG 
GGTTTGTGTT TGTCTTCATC TCTCAGACGA CAGTCGACGG CCACAGCAGC GGCGTTCAAC 
ACCACAGCCA CTCCAGAGAC TAAAGAGAGC AGAAAACCCA AAACAGGCAT TCTGATGCTG 
AATATGGGCG GTCCAGAGAA GCTGGAGGAT GTTCATGATT TCCTGCTGCG CCTCTTCATG 
GATACAGACT TCATGCAGCT GCCCGTGCAG AATAAACTCG GGCCATTCAT TGCCAAAAGA 
CGCACCCCAA AGATCCAGGA GCAGTACAGT AAGATTGGCG GAGGGTCTCC CATTAAAGCC 
TGGACCACCA TGCAGGGGGA AGGAATGGTG AAACTGCTGG ACGAGATGTG TCCGGACACG 
GCTCCTCATA AGTTTTACAT TGGTTTCCGG TACGTTCATC CGCTGACAGA GGAGGCCATA 
GAGTTGATGG AGAAAGATGG AGTGGAGAGA GCAGTGGCCT TCACACAGTA CCCACAGTAC 
AGCTGCTCCA CCACCGGCAG CAGTCTGAAC GCCATCTACA GATACTACAG TAATCGCGCC 
GACAGACCGA AGATGCGCTG GAGTGTGATT GACAGGTGGC CGACACACCC GCTACTGATA 
GAGTGTTTCG CGGAGCATGT TCGTAATGAG CTGGACAAGT TTCCTGTAGA GAAGAGAGAT 
GATGTAGTGA TTCTGTTCTC TGCGCACTCT CTGCCCCTAT CTGTGGTGAA CCGGGGTGAT 
CCATACCCAC AGGAAGTGGG CGCTACAGTT CAGAGAGTGA TGGACCGACT TGGCCACTGT 
AACCCATACA GACTCGTCTG GCAGTCAAAG GTTGGGCCGA TGGCGTGGTT GGGGCCACAG 
ACAGATGAGG TCATCAAGGG TCTCTGTCAG CGAGGCAAGA GGAACCTGCT GCTGGTGCCC 
ATCGCCTTCA CCAGCGACCA CATCGAGACG CTGCACGAGC TGGACATCGA GTACTCACAG 
GTCCTCGGAG AAGAAGTTGG AGTGGAAAAC ATCAGAAGAG CAGAATCTCT GAATGGAAAT 
CCACTGTTCT TCAGGGCTCT GGCTGATCTG GTTCAGTCTC ACCTGCAGTC TAATGAATCA 
TGCTCTCGTC AGCTGACCCT GCGCTGTCCG CTGTGTGTGA ACCCAACCTG TGCTCAAACC 
AAAGCCTTCT TCTCCAGCCA GAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fech ( XM_021468899.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324574.1 Danio rerio ferrochelatase (fech), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa150941
Clone ID Related Accession (Same CDS sequence)	XM_021468899.1
Accession Version	XM_021468899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ferrochelatase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTTT TAGGAGGCGC GTGCCGGCTC GTGCAGCTGG TCAGATGTGG TTCTCCTGTG 
GGTTTGTGTT TGTCTTCATC TCTCAGACGA CAGTCGACGG CCACAGCAGC GGCGTTCAAC 
ACCACAGCCA CTCCAGAGAC TAAAGAGAGC AGAAAACCCA AAACAGGCAT TCTGATGCTG 
AATATGGGCG GTCCAGAGAA GCTGGAGGAT GTTCATGATT TCCTGCTGCG CCTCTTCATG 
GATACAGACC TCATGCAGCT GCCCGTGCAG AATAAACTCG GGCCATTCAT TGCCAAAAGA 
CGCACCCCAA AGATCCAGGA GCAGTACAGT AAGATTGGCG GAGGGTCTCC TATTAAAGCC 
TGGACCACCA TGCAGGGGGA AGGAATGGTG AAACTGCTGG ATGAGATGTG TCCGGACACG 
GCTCCTCATA AGTTTTACAT TGGTTTCCGG TACGTTCATC CGCTGACGGA GGAGGCCATA 
GAGTTGATGG AGAAAGATGG AGTGGAGAGA GCTGTGGCCT TCACACAGTA CCCACAGTAC 
AGCTGCTCCA CCACCGGCAG CAGTCTGAAC GCCATCTACA GATACTACAG TAATCGCGCC 
GACAGACCGA AGATGCGCTG GAGTGTGATT GACAGGTGGC CGACACACCC GCTGCTGATA 
GAGTGTTTCG CGGAGCATGT TCGTAATGAG CTGGACAAGT TTCCTGTGGA GAAGAGAGAT 
GATGTGGTGA TTCTGTTCTC TGCGCACTCT CTGCCTCTAT CTGTGGTGAA CCGGGGTGAT 
CCATACCCAC AGGAAGTGGG CGCTACAGTT CAGAGAGTGA TGGACCGACT GGGCCACTGT 
AACCCATACA GACTCGTCTG GCAGTCAAAG GTTGGGCCGA TGGCGTGGTT GGGGCCACAG 
ACAGATGAGG TCATCAAGGG TCTCTGTCAG CGAGGCAAGA GGAACCTGCT GCTGGTGCCC 
ATCGCCTTCA CCAGCGACCA CATCGAGACG CTGCACGAGC TGGACATCGA GTACTCACAG 
GTCCTCGGAG AAGAGGTTGG AGTGGAAAAC ATCAGAAGAG CAGAATCTCT GAATGGAAAT 
CCACTGTTCT TCAGGGCTTT GGCTGATCTG GTTCAGTCTC ACCTGCAGTC TAATGAATCA 
TGCTCTCGTC AGCTGACCCT GCGCTGTCCG CTGTGTGTGA ACCCAACCTG TGCTCAAACC 
AAAGCCTTCT TCTCCAGCCA GAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324574.1
CDS	103..1332
Translation	MAVLGGACRLVQLVRCGSPVGLCLSSSLRRQSTATAAAFNTTATPETKESRKPKTGILMLNMGGPEKLEDVHDFLLRLFMDTDLMQLPVQNKLGPFIAKRRTPKIQEQYSKIGGGSPIKAWTTMQGEGMVKLLDEMCPDTAPHKFYIGFRYVHPLTEEAIELMEKDGVERAVAFTQYPQYSCSTTGSSLNAIYRYYSNRADRPKMRWSVIDRWPTHPLLIECFAEHVRNELDKFPVEKRDDVVILFSAHSLPLSVVNRGDPYPQEVGATVQRVMDRLGHCNPYRLVWQSKVGPMAWLGPQTDEVIKGLCQRGKRNLLLVPIAFTSDHIETLHELDIEYSQVLGEEVGVENIRRAESLNGNPLFFRALADLVQSHLQSNESCSRQLTLRCPLCVNPTCAQTKAFFSSQKL

Target ORF information:

RefSeq Version	XM_021468899.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ferrochelatase (fech), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468899.1

ORF Insert Sequence:

ATGGCGGTTT TAGGAGGCGC GTGCCGGCTC GTGCAGCTGG TCAGATGTGG TTCTCCTGTG 
GGTTTGTGTT TGTCTTCATC TCTCAGACGA CAGTCGACGG CCACAGCAGC GGCGTTCAAC 
ACCACAGCCA CTCCAGAGAC TAAAGAGAGC AGAAAACCCA AAACAGGCAT TCTGATGCTG 
AATATGGGCG GTCCAGAGAA GCTGGAGGAT GTTCATGATT TCCTGCTGCG CCTCTTCATG 
GATACAGACC TCATGCAGCT GCCCGTGCAG AATAAACTCG GGCCATTCAT TGCCAAAAGA 
CGCACCCCAA AGATCCAGGA GCAGTACAGT AAGATTGGCG GAGGGTCTCC TATTAAAGCC 
TGGACCACCA TGCAGGGGGA AGGAATGGTG AAACTGCTGG ATGAGATGTG TCCGGACACG 
GCTCCTCATA AGTTTTACAT TGGTTTCCGG TACGTTCATC CGCTGACGGA GGAGGCCATA 
GAGTTGATGG AGAAAGATGG AGTGGAGAGA GCTGTGGCCT TCACACAGTA CCCACAGTAC 
AGCTGCTCCA CCACCGGCAG CAGTCTGAAC GCCATCTACA GATACTACAG TAATCGCGCC 
GACAGACCGA AGATGCGCTG GAGTGTGATT GACAGGTGGC CGACACACCC GCTGCTGATA 
GAGTGTTTCG CGGAGCATGT TCGTAATGAG CTGGACAAGT TTCCTGTGGA GAAGAGAGAT 
GATGTGGTGA TTCTGTTCTC TGCGCACTCT CTGCCTCTAT CTGTGGTGAA CCGGGGTGAT 
CCATACCCAC AGGAAGTGGG CGCTACAGTT CAGAGAGTGA TGGACCGACT GGGCCACTGT 
AACCCATACA GACTCGTCTG GCAGTCAAAG GTTGGGCCGA TGGCGTGGTT GGGGCCACAG 
ACAGATGAGG TCATCAAGGG TCTCTGTCAG CGAGGCAAGA GGAACCTGCT GCTGGTGCCC 
ATCGCCTTCA CCAGCGACCA CATCGAGACG CTGCACGAGC TGGACATCGA GTACTCACAG 
GTCCTCGGAG AAGAGGTTGG AGTGGAAAAC ATCAGAAGAG CAGAATCTCT GAATGGAAAT 
CCACTGTTCT TCAGGGCTTT GGCTGATCTG GTTCAGTCTC ACCTGCAGTC TAATGAATCA 
TGCTCTCGTC AGCTGACCCT GCGCTGTCCG CTGTGTGTGA ACCCAACCTG TGCTCAAACC 
AAAGCCTTCT TCTCCAGCCA GAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Edwardsiella tarda-Induced Inhibition of Apoptosis: A Strategy for Intracellular Survival.
Frontiers in cellular and infection microbiology676(2016)
Zhou ZJ,Sun L