actb2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	actb2
Entrez Gene ID	57935
Full Name	actin, beta 2
Synonyms	actbb,bact2,bactin2,fa56g06,fb38a10,wu:fa56g06,wu:fb38a10
General protein information	Preferred Names actin, beta 2 Names actin, cytoplasmic 2 beta-actin-2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_181601.4	NP_853632.3	actin, cytoplasmic 2
NM_181601.5	NP_853632.3	actin, cytoplasmic 2

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	act-1	NP_505819.1
Schizosaccharomyces pombe (fission yeast)	act1	NP_595618.1
Gallus gallus (chicken)	ACTG1	NP_001007825.1
Anopheles gambiae (African malaria mosquito)	ACT5C_ANOGA	XP_003437050.1
Eremothecium gossypii	AGOS_ABR222W	NP_983171.1
Arabidopsis thaliana (thale cress)	ACT8	NP_175350.1
Oryza sativa (rice)	Os03g0718100	NP_001051086.1
Mus musculus (house mouse)	Actg1	NP_033739.1
Rattus norvegicus (Norway rat)	Actg1	NP_001120921.1
Danio rerio (zebrafish)	actb1	NP_571106.1
Caenorhabditis elegans (roundworm)	act-3	NP_505817.1
Caenorhabditis elegans (roundworm)	act-2	NP_505818.1
Kluyveromyces lactis	KLLA0D05357g	XP_453299.1
Magnaporthe oryzae (rice blast fungus)	MGG_03982	XP_003719871.1
Neurospora crassa	NCU04173	XP_961133.2
Homo sapiens (human)	ACTG1	NP_001605.1
Bos taurus (cattle)	ACTG1	NP_001028790.1
Danio rerio (zebrafish)	actb2	NP_853632.3
Drosophila melanogaster (fruit fly)	Act5C	NP_001014726.1
Saccharomyces cerevisiae (baker's yeast)	ACT1	NP_116614.1
	ACTG1	XP_001110892.2
Caenorhabditis elegans (roundworm)	act-4	NP_508841.1
Arabidopsis thaliana (thale cress)	ACT2	NP_188508.1
Xenopus tropicalis (tropical clawed frog)	actg1	NP_989332.1

Gene Ontology

The following actb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the actb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00108	NM_181601.5 Latest version!	Danio rerio actin, beta 2 (actb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa00108	NM_181601.4	Danio rerio actin, beta 2 (actb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

actb2 ( NM_181601.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_853632.3 Danio rerio actin, beta 2 (actb2), mRNA.

Price & Availability↑

CloneID	ODa00108
Clone ID Related Accession (Same CDS sequence)	NM_181601.4 , NM_181601.5
Accession Version	NM_181601.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Danio rerio(zebrafish)
Product	actin, cytoplasmic 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL929031.3 and BC154531.1. On Feb 12, 2013 this sequence version replaced NM_181601.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC045879.1, GDQH01000046.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA3505370 [ECO:0000348] ##Evidence-Data-END##

ATGGATGATG AAATTGCCGC ACTGGTTGTT GACAACGGAT CCGGTATGTG CAAAGCCGGA 
TTCGCTGGAG ATGATGCTCC CCGTGCTGTC TTCCCATCCA TCGTGGGTCG CCCCAGACAT 
CAGGGTGTCA TGGTTGGTAT GGGACAGAAA GACAGCTACG TTGGTGATGA GGCTCAGAGC 
AAGAGAGGTA TCCTGACCCT GAAGTACCCC ATCGAGCACG GTATTGTCAC CAACTGGGAT 
GATATGGAGA AGATCTGGCA TCACACCTTC TACAACGAGC TGCGTGTTGC CCCAGAGGAG 
CACCCCGTCC TGCTCACAGA GGCCCCCCTG AACCCCAAGG CCAACAGGGA AAAGATGACA 
CAGATCATGT TCGAGACCTT CAACACCCCC GCCATGTACG TTGCCATCCA GGCTGTGCTG 
TCCCTGTATG CCTCCGGTCG TACCACTGGT ATCGTGATGG ACTCTGGTGA TGGTGTCACC 
CACACTGTGC CCATCTACGA GGGTTACGCC CTGCCCCATG CCATCCTCCG TCTGGACTTG 
GCTGGCCGTG ACCTGACTGA CTACCTCATG AAGATCCTGA CCGAGAGAGG CTACAGCTTC 
ACCACCACAG CTGAGAGGGA AATTGTCCGT GACATCAAGG AGAAGCTCTG CTATGTGGCC 
CTTGACTTTG AGCAGGAGAT GGGCACCGCT GCTTCCTCCT CCTCCCTGGA GAAGAGCTAC 
GAGCTGCCTG ACGGACAGGT CATCACCATT GGCAATGAGA GGTTCAGGTG CCCAGAGGCC 
CTGTTCCAGC CATCCTTCTT GGGTATGGAA TCTTGCGGTA TCCACGAGAC CACCTTCAAC 
TCCATCATGA AGTGTGACGT CGACATCCGT AAGGACCTGT ATGCCAACAC TGTATTGTCT 
GGTGGTACCA CCATGTACCC TGGCATTGCT GACAGGATGC AGAAGGAGAT CACATCCCTG 
GCCCCTAGCA CAATGAAGAT CAAGATCATT GCCCCACCTG AGCGTAAATA CTCTGTCTGG 
ATCGGAGGCT CCATCCTGGC CTCCCTGTCC ACCTTCCAGC AGATGTGGAT TAGCAAGCAG 
GAGTACGATG AGTCTGGACC ATCCATCGTC CACCGCAAAT GCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_853632.3
CDS	98..1225
Translation	MDDEIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMGTAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITSLAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_181601.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actin, beta 2 (actb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181601.4

ORF Insert Sequence:

ATGGATGATG AAATTGCCGC ACTGGTTGTT GACAACGGAT CCGGTATGTG CAAAGCCGGA 
TTCGCTGGAG ATGATGCTCC CCGTGCTGTC TTCCCATCCA TCGTGGGTCG CCCCAGACAT 
CAGGGTGTCA TGGTTGGTAT GGGACAGAAA GACAGCTACG TTGGTGATGA GGCTCAGAGC 
AAGAGAGGTA TCCTGACCCT GAAGTACCCC ATCGAGCACG GTATTGTCAC CAACTGGGAT 
GATATGGAGA AGATCTGGCA TCACACCTTC TACAACGAGC TGCGTGTTGC CCCAGAGGAG 
CACCCCGTCC TGCTCACAGA GGCCCCCCTG AACCCCAAGG CCAACAGGGA AAAGATGACA 
CAGATCATGT TCGAGACCTT CAACACCCCC GCCATGTACG TTGCCATCCA GGCTGTGCTG 
TCCCTGTATG CCTCCGGTCG TACCACTGGT ATCGTGATGG ACTCTGGTGA TGGTGTCACC 
CACACTGTGC CCATCTACGA GGGTTACGCC CTGCCCCATG CCATCCTCCG TCTGGACTTG 
GCTGGCCGTG ACCTGACTGA CTACCTCATG AAGATCCTGA CCGAGAGAGG CTACAGCTTC 
ACCACCACAG CTGAGAGGGA AATTGTCCGT GACATCAAGG AGAAGCTCTG CTATGTGGCC 
CTTGACTTTG AGCAGGAGAT GGGCACCGCT GCTTCCTCCT CCTCCCTGGA GAAGAGCTAC 
GAGCTGCCTG ACGGACAGGT CATCACCATT GGCAATGAGA GGTTCAGGTG CCCAGAGGCC 
CTGTTCCAGC CATCCTTCTT GGGTATGGAA TCTTGCGGTA TCCACGAGAC CACCTTCAAC 
TCCATCATGA AGTGTGACGT CGACATCCGT AAGGACCTGT ATGCCAACAC TGTATTGTCT 
GGTGGTACCA CCATGTACCC TGGCATTGCT GACAGGATGC AGAAGGAGAT CACATCCCTG 
GCCCCTAGCA CAATGAAGAT CAAGATCATT GCCCCACCTG AGCGTAAATA CTCTGTCTGG 
ATCGGAGGCT CCATCCTGGC CTCCCTGTCC ACCTTCCAGC AGATGTGGAT TAGCAAGCAG 
GAGTACGATG AGTCTGGACC ATCCATCGTC CACCGCAAAT GCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

actb2 ( NM_181601.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_853632.3 Danio rerio actin, beta 2 (actb2), mRNA.

Price & Availability↑

CloneID	ODa00108
Clone ID Related Accession (Same CDS sequence)	NM_181601.4 , NM_181601.5
Accession Version	NM_181601.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Danio rerio(zebrafish)
Product	actin, cytoplasmic 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL929031.3. On Oct 14, 2018 this sequence version replaced NM_181601.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC154531.1, GDQH01000046.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA3505370 [ECO:0000350] ##Evidence-Data-END##

ATGGATGATG AAATTGCCGC ACTGGTTGTT GACAACGGAT CCGGTATGTG CAAAGCCGGA 
TTCGCTGGAG ATGATGCTCC CCGTGCTGTC TTCCCATCCA TCGTGGGTCG CCCCAGACAT 
CAGGGTGTCA TGGTTGGTAT GGGACAGAAA GACAGCTACG TTGGTGATGA GGCTCAGAGC 
AAGAGAGGTA TCCTGACCCT GAAGTACCCC ATCGAGCACG GTATTGTCAC CAACTGGGAT 
GATATGGAGA AGATCTGGCA TCACACCTTC TACAACGAGC TGCGTGTTGC CCCAGAGGAG 
CACCCCGTCC TGCTCACAGA GGCCCCCCTG AACCCCAAGG CCAACAGGGA AAAGATGACA 
CAGATCATGT TCGAGACCTT CAACACCCCC GCCATGTACG TTGCCATCCA GGCTGTGCTG 
TCCCTGTATG CCTCCGGTCG TACCACTGGT ATCGTGATGG ACTCTGGTGA TGGTGTCACC 
CACACTGTGC CCATCTACGA GGGTTACGCC CTGCCCCATG CCATCCTCCG TCTGGACTTG 
GCTGGCCGTG ACCTGACTGA CTACCTCATG AAGATCCTGA CCGAGAGAGG CTACAGCTTC 
ACCACCACAG CTGAGAGGGA AATTGTCCGT GACATCAAGG AGAAGCTCTG CTATGTGGCC 
CTTGACTTTG AGCAGGAGAT GGGCACCGCT GCTTCCTCCT CCTCCCTGGA GAAGAGCTAC 
GAGCTGCCTG ACGGACAGGT CATCACCATT GGCAATGAGA GGTTCAGGTG CCCAGAGGCC 
CTGTTCCAGC CATCCTTCTT GGGTATGGAA TCTTGCGGTA TCCACGAGAC CACCTTCAAC 
TCCATCATGA AGTGTGACGT CGACATCCGT AAGGACCTGT ATGCCAACAC TGTATTGTCT 
GGTGGTACCA CCATGTACCC TGGCATTGCT GACAGGATGC AGAAGGAGAT CACATCCCTG 
GCCCCTAGCA CAATGAAGAT CAAGATCATT GCCCCACCTG AGCGTAAATA CTCTGTCTGG 
ATCGGAGGCT CCATCCTGGC CTCCCTGTCC ACCTTCCAGC AGATGTGGAT TAGCAAGCAG 
GAGTACGATG AGTCTGGACC ATCCATCGTC CACCGCAAAT GCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_853632.3
CDS	111..1238
Translation	MDDEIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMGTAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITSLAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_181601.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actin, beta 2 (actb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181601.5

ORF Insert Sequence:

ATGGATGATG AAATTGCCGC ACTGGTTGTT GACAACGGAT CCGGTATGTG CAAAGCCGGA 
TTCGCTGGAG ATGATGCTCC CCGTGCTGTC TTCCCATCCA TCGTGGGTCG CCCCAGACAT 
CAGGGTGTCA TGGTTGGTAT GGGACAGAAA GACAGCTACG TTGGTGATGA GGCTCAGAGC 
AAGAGAGGTA TCCTGACCCT GAAGTACCCC ATCGAGCACG GTATTGTCAC CAACTGGGAT 
GATATGGAGA AGATCTGGCA TCACACCTTC TACAACGAGC TGCGTGTTGC CCCAGAGGAG 
CACCCCGTCC TGCTCACAGA GGCCCCCCTG AACCCCAAGG CCAACAGGGA AAAGATGACA 
CAGATCATGT TCGAGACCTT CAACACCCCC GCCATGTACG TTGCCATCCA GGCTGTGCTG 
TCCCTGTATG CCTCCGGTCG TACCACTGGT ATCGTGATGG ACTCTGGTGA TGGTGTCACC 
CACACTGTGC CCATCTACGA GGGTTACGCC CTGCCCCATG CCATCCTCCG TCTGGACTTG 
GCTGGCCGTG ACCTGACTGA CTACCTCATG AAGATCCTGA CCGAGAGAGG CTACAGCTTC 
ACCACCACAG CTGAGAGGGA AATTGTCCGT GACATCAAGG AGAAGCTCTG CTATGTGGCC 
CTTGACTTTG AGCAGGAGAT GGGCACCGCT GCTTCCTCCT CCTCCCTGGA GAAGAGCTAC 
GAGCTGCCTG ACGGACAGGT CATCACCATT GGCAATGAGA GGTTCAGGTG CCCAGAGGCC 
CTGTTCCAGC CATCCTTCTT GGGTATGGAA TCTTGCGGTA TCCACGAGAC CACCTTCAAC 
TCCATCATGA AGTGTGACGT CGACATCCGT AAGGACCTGT ATGCCAACAC TGTATTGTCT 
GGTGGTACCA CCATGTACCC TGGCATTGCT GACAGGATGC AGAAGGAGAT CACATCCCTG 
GCCCCTAGCA CAATGAAGAT CAAGATCATT GCCCCACCTG AGCGTAAATA CTCTGTCTGG 
ATCGGAGGCT CCATCCTGGC CTCCCTGTCC ACCTTCCAGC AGATGTGGAT TAGCAAGCAG 
GAGTACGATG AGTCTGGACC ATCCATCGTC CACCGCAAAT GCTTCTAA