gfpt2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gfpt2
Entrez Gene ID	569945
Full Name	glutamine-fructose-6-phosphate transaminase 2
General protein information	Preferred Names glutamine-fructose-6-phosphate transaminase 2 Names glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001033921.1	NP_001029093.1	glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	GFPT2	XP_003642114.1
Drosophila melanogaster (fruit fly)	Gfat1	NP_001015157.2
Saccharomyces cerevisiae (baker's yeast)	GFA1	NP_012818.1
Eremothecium gossypii	AGOS_ABL036C	NP_982911.2
Oryza sativa (rice)	Os12g0131100	NP_001066074.1
	GFPT2	XP_001106226.2
Canis lupus familiaris (dog)	GFPT2	XP_005626343.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011352	XP_309298.2
Neurospora crassa	NCU11350	XP_957994.2
Pan troglodytes (chimpanzee)	GFPT2	XP_518158.4
Rattus norvegicus (Norway rat)	Gfpt2	NP_001002819.2
Caenorhabditis elegans (roundworm)	F22B3.4	NP_502156.1
Arabidopsis thaliana (thale cress)	AT3G24090	NP_189051.3
Drosophila melanogaster (fruit fly)	Gfat2	NP_651617.1
Caenorhabditis elegans (roundworm)	F07A11.2	NP_496479.1
Kluyveromyces lactis	KLLA0F13596g	XP_455692.1
Homo sapiens (human)	GFPT2	NP_005101.1
Bos taurus (cattle)	GFPT2	NP_001070351.1
Mus musculus (house mouse)	Gfpt2	NP_038557.1
Danio rerio (zebrafish)	gfpt2	NP_001029093.1
Schizosaccharomyces pombe (fission yeast)	SPBC12C2.11	NP_596011.1
Magnaporthe oryzae (rice blast fungus)	MGG_11597	XP_003718755.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetically Blocking the Zebrafish Pineal Clock Affects Circadian Behavior.
Ben-Moshe Livne Z, Alon S, Vallone D, Bayleyen Y, Tovin A, Shainer I, Nisembaum LG, Aviram I, Smadja-Storz S, Fuentes M, Falc?n J, Eisenberg E, Klein DC, Burgess HA, Foulkes NS, Gothilf Y
PLoS genetics12(11)e1006445(2016 Nov)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Mutations in gfpt1 and skiv2l2 cause distinct stage-specific defects in larval melanocyte regeneration in zebrafish.
Yang CT, Hindes AE, Hultman KA, Johnson SL
PLoS genetics3(6)e88(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gfpt2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gfpt2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa32813	NM_001033921.1 Latest version!	Danio rerio glutamine-fructose-6-phosphate transaminase 2 (gfpt2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gfpt2 ( NM_001033921.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001029093.1 Danio rerio glutamine-fructose-6-phosphate transaminase 2 (gfpt2), mRNA.

Price & Availability↑

CloneID	ODa32813
Clone ID Related Accession (Same CDS sequence)	NM_001033921.1
Accession Version	NM_001033921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ173929.1. On Oct 1, 2005 this sequence version replaced XM_693363.1. ##Evidence-Data-START## Transcript exon combination :: DQ173929.1, BC163281.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTGCGGAA TTTTCGCCTA CCTGAATTAC AGAGTTCCAC GAAAGAGGAG GGATATCTTT 
GAGACACTTG TAAAGGGACT TCAAAGGCTG GAGTACAGGG GTTATGACTC CGCAGGTATT 
GCAGTAGATG GCCCTGTGAA AGATGACGAA GCTAAAATCT GCTTATATAA GAAGACCGGG 
AAGGTGAAAG CTCTTAATGA AGAGATCTAC AAGAATGACT CCATTGACCT CGAAGCAGAA 
CTTGACACAC ACTTTGGAAT TGCACACACT CGCTGGGCGA CACACGGAGA GCCCAGTCCT 
CTCAACAGCC ACCCTCACCG ATCCGACAAG AACAACGAGT TTGTTGTCAT CCACAATGGC 
ATCATCACAA ATTACAAGGA GCTGAAAAAG TACCTGGGTT CCAAAGGCTA TGAGTTTGAG 
TCGGACACAG ACACGGAGGT CATCCCTAAG TTGATCAAGT ACCTCTATGA CAACCGAGAG 
AACGATTATG TGTCCTTCTC CACCCTAGTG GAGCGCGTCA TCAAGCAGCT GGAAGGAGCA 
TTTGCTCTGG TTTTCAAAAG CATTCATTTC CCAGGAGAGG CTGTTGCCAC CAGGAGAGGC 
AGTCCACTGC TCATCGGCAT CCGCAGTCAA TATGAACTTT CCACAGAGCA GATTCCTGTC 
CAGTACAACT GCGGTGATTC CAGAAGAGAT GAACAAGAGA AAAACCTGCA TAACGGAGTG 
GGAAGCTCTA ATGCCCTGCA CTCAATTGAC GACAGACTGG CGGTGGAGTT TTACTTTGCT 
TCTGATGCCA GTGCCATCAT TGAGCACACC AACAAGGTGA TTTATGTGGA GGACGATGAC 
GTTGCTGTAG TGATGGAGGG GAAGCTCTAT GTGCACCGCG TTAAGCGAGC GGCTGGAGAA 
GATCCTGTCC GTGTCATTCA AACCCTTCAG ATGGAGCTCC AGCAGATCAT GAAAGGTAAC 
TTCAAGGCCT TCATGCAGAA GGAGATCTTT GAACAGCCTG AATCTGTCTA CAACACCATG 
AGAGGCAGAA TCTTTTTTGA CCCCAACACA GTTTTTCTCG GGGGTTTGAC TGACCATCTG 
AAAGAGATCA AAAGATGCAG ACGCCTGATC ATGATTGGCT GCGGGACCAG TTATCATGCT 
GGAGTGGCGA CTCGACAGAT CCTGGAGGAG TTGACTGAGC TTCCTGTTAT GGTGGAGCTG 
GCCAGTGATT TCCTGGATCG CATCACTCCT GTCTTCAGAG ATGACGTCTG TTTCTTCATC 
AGCCAGTCGG GCGAGACGGC GGACACTCTG ATGGCTCTCC GCTACTGTAA GAAGCGTGGG 
GCATTGACTG TGGGCATCAC CAACACTGTG GGCAGCTCAA TCTGCAGAGA GACCGACTGT 
GGGGTTCACA TCAATGCTGG GCCTGAGGTC GGGGTCGCCA GCACTAAGGC CTACACCAGC 
CAGTTTGTGT CTCTGATCAT GTTCGGCCTG ATGATGTGTG AAGACAGACT TTCACTGGAG 
CCGAGGAGAC AGGAGATCAT CAGTGGCCTC AATCAACTGC CAGAGTTGAT CAAGAAAGTT 
CTGGCTCAGG ATGACAATAT CAAAACCATT GCAGAGGAGC TTCACCATCA GAGGTCTCTA 
TTAGTCATGG GTCGAGGCTT CAACTACGCC ACCTGTCTAG AGGGGGCGCT GAAAATCAAA 
GAAATCACAT ACATGCATTC AGAGGGCATT CTGGCTGGAG AGCTGAAGCA CGGCCCATTG 
GCACTCATCG ACAAGCACAT GCCAGTCATC ATGATCATTA TGAGAGACGC CTGCTACCAA 
AAGTGTCATA ATGCCTTGCA ACAGGTCACA GCCAGACAGG GTCGCCCCAT CATCATATGC 
TGCCAGGACG ACCCAGAGAT CAGCAAAATG GCCTATAAGA CTATTGAACT GCCTCAAACA 
GTGGACTGTC TGCAGGGCAT CCTCAGTGTC ATTCCTCTGC AGCTCATATC GTTCCATCTC 
GCCGTTCTCA GGGGATATGA TGTGGACTGC CCGAGGAACC TGGCCAAATC TGTGACAGTG 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029093.1
CDS	30..2075
Translation	MCGIFAYLNYRVPRKRRDIFETLVKGLQRLEYRGYDSAGIAVDGPVKDDEAKICLYKKTGKVKALNEEIYKNDSIDLEAELDTHFGIAHTRWATHGEPSPLNSHPHRSDKNNEFVVIHNGIITNYKELKKYLGSKGYEFESDTDTEVIPKLIKYLYDNRENDYVSFSTLVERVIKQLEGAFALVFKSIHFPGEAVATRRGSPLLIGIRSQYELSTEQIPVQYNCGDSRRDEQEKNLHNGVGSSNALHSIDDRLAVEFYFASDASAIIEHTNKVIYVEDDDVAVVMEGKLYVHRVKRAAGEDPVRVIQTLQMELQQIMKGNFKAFMQKEIFEQPESVYNTMRGRIFFDPNTVFLGGLTDHLKEIKRCRRLIMIGCGTSYHAGVATRQILEELTELPVMVELASDFLDRITPVFRDDVCFFISQSGETADTLMALRYCKKRGALTVGITNTVGSSICRETDCGVHINAGPEVGVASTKAYTSQFVSLIMFGLMMCEDRLSLEPRRQEIISGLNQLPELIKKVLAQDDNIKTIAEELHHQRSLLVMGRGFNYATCLEGALKIKEITYMHSEGILAGELKHGPLALIDKHMPVIMIIMRDACYQKCHNALQQVTARQGRPIIICCQDDPEISKMAYKTIELPQTVDCLQGILSVIPLQLISFHLAVLRGYDVDCPRNLAKSVTVE

Target ORF information:

RefSeq Version	NM_001033921.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glutamine-fructose-6-phosphate transaminase 2 (gfpt2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033921.1

ORF Insert Sequence:

ATGTGCGGAA TTTTCGCCTA CCTGAATTAC AGAGTTCCAC GAAAGAGGAG GGATATCTTT 
GAGACACTTG TAAAGGGACT TCAAAGGCTG GAGTACAGGG GTTATGACTC CGCAGGTATT 
GCAGTAGATG GCCCTGTGAA AGATGACGAA GCTAAAATCT GCTTATATAA GAAGACCGGG 
AAGGTGAAAG CTCTTAATGA AGAGATCTAC AAGAATGACT CCATTGACCT CGAAGCAGAA 
CTTGACACAC ACTTTGGAAT TGCACACACT CGCTGGGCGA CACACGGAGA GCCCAGTCCT 
CTCAACAGCC ACCCTCACCG ATCCGACAAG AACAACGAGT TTGTTGTCAT CCACAATGGC 
ATCATCACAA ATTACAAGGA GCTGAAAAAG TACCTGGGTT CCAAAGGCTA TGAGTTTGAG 
TCGGACACAG ACACGGAGGT CATCCCTAAG TTGATCAAGT ACCTCTATGA CAACCGAGAG 
AACGATTATG TGTCCTTCTC CACCCTAGTG GAGCGCGTCA TCAAGCAGCT GGAAGGAGCA 
TTTGCTCTGG TTTTCAAAAG CATTCATTTC CCAGGAGAGG CTGTTGCCAC CAGGAGAGGC 
AGTCCACTGC TCATCGGCAT CCGCAGTCAA TATGAACTTT CCACAGAGCA GATTCCTGTC 
CAGTACAACT GCGGTGATTC CAGAAGAGAT GAACAAGAGA AAAACCTGCA TAACGGAGTG 
GGAAGCTCTA ATGCCCTGCA CTCAATTGAC GACAGACTGG CGGTGGAGTT TTACTTTGCT 
TCTGATGCCA GTGCCATCAT TGAGCACACC AACAAGGTGA TTTATGTGGA GGACGATGAC 
GTTGCTGTAG TGATGGAGGG GAAGCTCTAT GTGCACCGCG TTAAGCGAGC GGCTGGAGAA 
GATCCTGTCC GTGTCATTCA AACCCTTCAG ATGGAGCTCC AGCAGATCAT GAAAGGTAAC 
TTCAAGGCCT TCATGCAGAA GGAGATCTTT GAACAGCCTG AATCTGTCTA CAACACCATG 
AGAGGCAGAA TCTTTTTTGA CCCCAACACA GTTTTTCTCG GGGGTTTGAC TGACCATCTG 
AAAGAGATCA AAAGATGCAG ACGCCTGATC ATGATTGGCT GCGGGACCAG TTATCATGCT 
GGAGTGGCGA CTCGACAGAT CCTGGAGGAG TTGACTGAGC TTCCTGTTAT GGTGGAGCTG 
GCCAGTGATT TCCTGGATCG CATCACTCCT GTCTTCAGAG ATGACGTCTG TTTCTTCATC 
AGCCAGTCGG GCGAGACGGC GGACACTCTG ATGGCTCTCC GCTACTGTAA GAAGCGTGGG 
GCATTGACTG TGGGCATCAC CAACACTGTG GGCAGCTCAA TCTGCAGAGA GACCGACTGT 
GGGGTTCACA TCAATGCTGG GCCTGAGGTC GGGGTCGCCA GCACTAAGGC CTACACCAGC 
CAGTTTGTGT CTCTGATCAT GTTCGGCCTG ATGATGTGTG AAGACAGACT TTCACTGGAG 
CCGAGGAGAC AGGAGATCAT CAGTGGCCTC AATCAACTGC CAGAGTTGAT CAAGAAAGTT 
CTGGCTCAGG ATGACAATAT CAAAACCATT GCAGAGGAGC TTCACCATCA GAGGTCTCTA 
TTAGTCATGG GTCGAGGCTT CAACTACGCC ACCTGTCTAG AGGGGGCGCT GAAAATCAAA 
GAAATCACAT ACATGCATTC AGAGGGCATT CTGGCTGGAG AGCTGAAGCA CGGCCCATTG 
GCACTCATCG ACAAGCACAT GCCAGTCATC ATGATCATTA TGAGAGACGC CTGCTACCAA 
AAGTGTCATA ATGCCTTGCA ACAGGTCACA GCCAGACAGG GTCGCCCCAT CATCATATGC 
TGCCAGGACG ACCCAGAGAT CAGCAAAATG GCCTATAAGA CTATTGAACT GCCTCAAACA 
GTGGACTGTC TGCAGGGCAT CCTCAGTGTC ATTCCTCTGC AGCTCATATC GTTCCATCTC 
GCCGTTCTCA GGGGATATGA TGTGGACTGC CCGAGGAACC TGGCCAAATC TGTGACAGTG 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetically Blocking the Zebrafish Pineal Clock Affects Circadian Behavior.
PLoS genetics12(11)e1006445(2016 Nov)
Ben-Moshe Livne Z,Alon S,Vallone D,Bayleyen Y,Tovin A,Shainer I,Nisembaum LG,Aviram I,Smadja-Storz S,Fuentes M,Falc?n J,Eisenberg E,Klein DC,Burgess HA,Foulkes NS,Gothilf Y

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Mutations in gfpt1 and skiv2l2 cause distinct stage-specific defects in larval melanocyte regeneration in zebrafish.
PLoS genetics3(6)e88(2007 Jun)
Yang CT,Hindes AE,Hultman KA,Johnson SL