cacna1ab gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021479643.1	XP_021335318.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X2
XM_021479647.1	XP_021335322.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X6
XM_017358346.2	XP_017213835.2	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X8
XM_021479652.1	XP_021335327.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X12
XM_021479654.1	XP_021335329.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X14
XM_017358346.1	XP_017213835.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X2
XM_021479649.1	XP_021335324.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X9
XM_021479653.1	XP_021335328.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X13
XM_021479655.1	XP_021335330.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X15
NM_001328708.1	NP_001315637.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A
XM_021479642.1	XP_021335317.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X1
XM_021479644.1	XP_021335319.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X3
XM_021479645.1	XP_021335320.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X4
XM_021479656.1	XP_021335331.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X16
XM_021479646.1	XP_021335321.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X5
XM_021479648.1	XP_021335323.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X7
XM_021479650.1	XP_021335325.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X10
XM_021479651.1	XP_021335326.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X11

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa98295	NM_001328708.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa147893	XM_021479642.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa147894	XM_021479643.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa147895	XM_021479644.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa147896	XM_021479645.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa147897	XM_021479646.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa147898	XM_021479647.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa147899	XM_021479648.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa147900	XM_017358346.2 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa147901	XM_021479649.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa147902	XM_021479650.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa147903	XM_021479651.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa147904	XM_021479652.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa147905	XM_021479653.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa147906	XM_021479654.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa147907	XM_021479655.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa147908	XM_021479656.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa54361	XM_017358346.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

cacna1ab ( NM_001328708.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001315637.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), mRNA.

Price & Availability↑

CloneID	ODa98295
Clone ID Related Accession (Same CDS sequence)	NM_001328708.1
Accession Version	NM_001328708.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from KC192783.1. On Jun 28, 2016 this sequence version replaced XM_017358345.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: KC192783.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476764, SAMN01765705 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGGG GAGCGGTAAG GGGAAAGACA TCAGTATCAT CAAGTCTCTG 
AGGGTTCTAA GGGTGCTGCG TCCACTCAAG ACCATCAAAC GTCTTCCAAA ACTAAAAGCG 
GTGTTTGACT GTGTAGTAAA CTCTCTGAAG AATGTGTTGA ACATCCTCAT TGTCTACATG 
CTGTTCATGT TCATCTTCGC TGTGGTGGCC GTGCAGCTTT TTAAAGGACG ATTCTTTTAC 
TGCACTGATG AGTCTAAAGA GTTTGCCCGA GACTGCAGGG GCGAATATTT GGTGTACGAG 
CGCGATAACG AGGTCAAATC TCAGAAGAGG GAGTGGAAGA AGTACGATTT CCACTACGAC 
AATGTGCTTT GGGCCCTGCT TACTCTCTTT ACTGTGTCCA CCGGAGAGGG ATGGCCAGAT 
GTGTTGAAGC ACTCTGTGGA CTCCACTTAC GAGAATCGGG GCCCGAGCCC GGGATACAGG 
ATGGAAATGT CCATCTTTTA CGTGGTGTAC TTCGTTGTCT TCCCCTTTTT CTTCGTAAAC 
ATATTTGTAG CTCTCATCAT CATCACCTTC CAAGAGCAAG GAGACAAGAT GATGGAGGAC 
TACAGCCTCG AAAAAAATGA GAGAGCCTGT ATTGATTTTG CTATAAATGC CAAACCTCTA 
ACGCGCCACA TGCCTCAGAA CAAGCAAAGC TTTCAGTACC GAATGTGGCA GTTTGTGGTG 
TCGCCTCCGT TTGAGTACAG TATCATGTCT CTCATTGCGC TCAACACCAT CATCCTTATG 
ATGAAGTATA ATGGGGCTTC CGATGCATAT GACAAAGTCC TGAAGAACCT GAATATAGTG 
TTCACCACAT TCTTCTTCAT GGAGAGTATT CTGAAAATCA TTGCCTTTGG TGTCAGAAAT 
TTTTTCAGAG ATGCTTGGAA TATTTTTGAT TTTGTGTCTG TTATCGGCAG CATCACTGAT 
ATTTTAGTGA CTGAGCTCGT GAATAACTTC ATAAACCTGA GTTTTCTGAG ACTCTTTCGA 
GCAGCTCGTC TCATTAAGCT CTTGCGGCAG GGCGAGACCA TTCGCATTCT GCTTTGGACC 
TTCGTTCAGT CATTTAAGGC TCTGCCATAT GTATGCCTGC TCATTGCAAT TCTGTTTTTC 
ATCTACGCCA TCATTGGCAT GCAGCTGTTT GGGAACTTGG CTCTGGATGA CGAAGGTGCT 
ATCAATGAAC ACAACAACTT CAGAACCTTC TTCATGGCCC TCATGCTCCT CTTCAGGAGT 
GCCACGGGCG AGGCGTGGCA TGATATCATG CTGTCATGTC TGGGAGGAAT GCCCTGTGAG 
CCGGAGGAGG TTGGATCAGA ATGTGGCAGC AATGTGGCCT ATGCATACTT CGTCTCTTTC 
ATCTTTCTCT GTTCGTTCCT GATGCTGAAT CTTTTTGTGG CTGTCATCAT GGATAACTTT 
GAGTACTTGA CCAGGGACTC CTCCATCCTG GGTCCGCATC ATTTGGATGA GTATGTCAGG 
ATATGGGCAG AGTACGACCC CGCAGCCTGC GGGCGCATTC ATTATAAGGA TATGTACAGT 
TTATTGCGGG TTATCGACCC CCCGCTGGGC TTAGGCAAGA AATGTCCTCA TAGGGTGGCC 
TGCAAGAGAC TGCTCCGTAT GGACCTGCCC GTTGCTGATG ACAACACGGT GCATTTCAAC 
TCCACTCTGA TGGCCTTGAT TCGCACAGCA CTGGACATCA AAATTGCCAA GGGAGGAGCG 
GACAAACACC AGATGGACGC GGAGCTCCGT AAAGAAATGA TGGCGATTTG GCCAAATCTC 
TCTCAGAAGA ACCTGGATCT GCTGGTAACG CCACACAAGT CAGCGACAGA CCTGACAGTG 
GGCAAGATCT ACGCCGCCAT GATGATCATG GAGTACTACC GGCAGAGCAA GGCCAAAAAG 
CTGCAGGCGC TTCGTGAGGA GCAGAACCGA ACGCCATTAA TGTTTCAGCG CATGGAGCCG 
TCCCCAAACC AGGAAGGGGG ACCGGGGCTG AATGGCCTGC CCGAGCTGCC GCAGGAGCCC 
GTTAACAGCC TGCCTCCAGA GGTTGGAATG AATGAAAGCC AGTCATGGGT GACGTCACGC 
GCCCAGGAGA TGTGCCAGAA GTCCGTTCCA GAAAACTGGA ACCCTGACAG ACCATATCCT 
GATGACCATC ATGACAGCCG CCACAATCCA CAGACCATCA CCGACACAAG TCCAATGCGT 
CGTTCTACAT CCTCATTGGT CCATGGACGT TCAGGTCGAG GCGTGCGATT GGATGATTAC 
TCTCTGGAAC GGGTGGTGTC AGAAGAGGGT CACCGACATG CTCCCCGAAG GAGAGATCGT 
GATCGAGACC GGGATAGAGA TCGCAGTCAT CGAACCTCAG AGAGATCCCT CGCTCGCTAT 
ACAGATGCAG ACACAGGTCT GGGTACAGAT CTTAGCACCA CTACACAGTC GGGTGACCTC 
CCACCTAAAG AAAGGGAACG AGAACGGGGT CGAGCAAAAG ACCGTCGCCA CCACCACCAC 
CATCATCACC ACCACGGCTC GGTGGATAAA GATCGGTACA GTCACGAGCG CGACTACCAT 
CGTCATCCTC AAGACAGATC AGATCGCCAA TGGTCCCGCT CTCCGAGTGA GGGCAGAGGG 
CACAGACAGG GCAGTAGTTC TGTGAGCGGA AGCCCCGTTC CCTCGACGTC GGGGACCAGC 
ACCCCCCGGC GTGGCCGTCG CCAGTTGCCC CAGACCCCAT CCACTCCACG CCCTCACGTC 
ACCTACTCCC CTGCGGTCCG CAAGCCTCCA TATGGCTCCC CAGGCCAGGG TCGACTCCGC 
TCCCCTTCCC CGCGAAACTT TTCTCCGCCA GGTCATGAAC CGCCATACCA CCGTCCACCC 
TCCCGCCATG CCTCACCTCA TCCTGGCTCA CCCCGGCATG CCTCTCCGCG TTCCCCCAGG 
CACACGTCGC CCAGGTCCCC TCGTCACGGC CGCTGGGGCC CTCCGGACAG CCTAGAAGGT 
GATGGAACTT TCTACGAACG GGACTATGAG TACGAGCGCC ACCATGAGCC GCCAGCCTAC 
GAACAGAGTT TATCACAGGG CAACCCCCAT CCACATTCAC ACCCTCATCC CCGGTCACCA 
AGGACTGCTC GTCACGGGCC TCAACCACAC CCGCGGCGTA TGCCTAACGG CTACCGTTCC 
TCCTCGCCCT CCCCGCACCG GCGCGGCCCC CACCCAGGCC CACACCGGCC ACCGCATGGC 
CGTGGGCCCC GGAAGGGGTT GCACGAACAA TATAGCGAGA CGGACGAGGA TGATTGGTGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315637.1
CDS	1..6843
Translation	MARFGDEVPARYGGGGGGGSGQGGPGRGGSRQGGPPGAQRMYKQSMAQRARTMAIYNPIPVRQNCLTVNRSLFLFSEDNIVRKYAKKITEWPPFEWMILTTIIANCIVLALEQHLPVGDKTPLSERLEETEPYFIAIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSSVGSELDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGVLLFVAILMFAIIGLEFYMGKFHTTCFNTITGEMYDERPCGARTCDANNTQCQGYWLGPNYGITQFDNVLFAVLTVFQCITMEGWTDMLYYSNDALGSAWNWMYYVPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILAEDDNDTGSRRRPTLKKNKADLLNPQEGVDHMGDSVGSPFARGSMSGKGDETPFSKKERRLRFLIRKMVKTQAFYWTVISLVALNTLCVAVVHYDQPELLSDFLYYAEFIFLGLFMSEMLIKMYGLGTRPYFHSSFNCFDCAVIVGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFELGTPPTNFDTFPAAIMTVFQILTGEDWNVVMYDGIKSQGGVDKGMIFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEATTQKMALQKAKEVAEVSPLSAANLSIAAKEQQKNSKGSKSVWEQRTSEIRRQNLMTSREALYNELDAEDHWKMNYRNRPDVKTHLDRPLVVNPQENRNNNTNKPRPDDPNPQERSEEHPSRSHHHHSHHHHHHHHHTHHSSRQSLHLPELAETGFECDVEGAGEHRRPRHHQHRRRDEDGGERGRKHRDHHSRGETAGGDAEDGQEKRQRRHRHANHGDGDGRRERSRLHRNKKEGSLSTARPIQPGLPHTESQYSEDLDNLRNSSKMAIHDPDPDLYDPYYDPEQANNLLNLRGPDLHDSSLILTDSSKLEHTAIDMPPIYPSLNAKLQVNKNANTEPAKEGEKKEEEEDDGGDEEGHKRIPPFSSCFILSTTNPFRRCCHYILTLRYFEMCILSVIAMSSIALAAEDPVSQDSERNKVLRYFDYVFTGVFTFEMLIKMVVLGLILHEGSYFRDLWNFLDFVVVSGALVAFAFTGSGKGKDISIIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFARDCRGEYLVYERDNEVKSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPDVLKHSVDSTYENRGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYSIMSLIALNTIILMMKYNGASDAYDKVLKNLNIVFTTFFFMESILKIIAFGVRNFFRDAWNIFDFVSVIGSITDILVTELVNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAILFFIYAIIGMQLFGNLALDDEGAINEHNNFRTFFMALMLLFRSATGEAWHDIMLSCLGGMPCEPEEVGSECGSNVAYAYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKKLQALREEQNRTPLMFQRMEPSPNQEGGPGLNGLPELPQEPVNSLPPEVGMNESQSWVTSRAQEMCQKSVPENWNPDRPYPDDHHDSRHNPQTITDTSPMRRSTSSLVHGRSGRGVRLDDYSLERVVSEEGHRHAPRRRDRDRDRDRDRSHRTSERSLARYTDADTGLGTDLSTTTQSGDLPPKERERERGRAKDRRHHHHHHHHHGSVDKDRYSHERDYHRHPQDRSDRQWSRSPSEGRGHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPSTPRPHVTYSPAVRKPPYGSPGQGRLRSPSPRNFSPPGHEPPYHRPPSRHASPHPGSPRHASPRSPRHTSPRSPRHGRWGPPDSLEGDGTFYERDYEYERHHEPPAYEQSLSQGNPHPHSHPHPRSPRTARHGPQPHPRRMPNGYRSSSPSPHRRGPHPGPHRPPHGRGPRKGLHEQYSETDEDDWC

Target ORF information:

RefSeq Version	NM_001328708.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328708.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGGG GAGCGGTAAG GGGAAAGACA TCAGTATCAT CAAGTCTCTG 
AGGGTTCTAA GGGTGCTGCG TCCACTCAAG ACCATCAAAC GTCTTCCAAA ACTAAAAGCG 
GTGTTTGACT GTGTAGTAAA CTCTCTGAAG AATGTGTTGA ACATCCTCAT TGTCTACATG 
CTGTTCATGT TCATCTTCGC TGTGGTGGCC GTGCAGCTTT TTAAAGGACG ATTCTTTTAC 
TGCACTGATG AGTCTAAAGA GTTTGCCCGA GACTGCAGGG GCGAATATTT GGTGTACGAG 
CGCGATAACG AGGTCAAATC TCAGAAGAGG GAGTGGAAGA AGTACGATTT CCACTACGAC 
AATGTGCTTT GGGCCCTGCT TACTCTCTTT ACTGTGTCCA CCGGAGAGGG ATGGCCAGAT 
GTGTTGAAGC ACTCTGTGGA CTCCACTTAC GAGAATCGGG GCCCGAGCCC GGGATACAGG 
ATGGAAATGT CCATCTTTTA CGTGGTGTAC TTCGTTGTCT TCCCCTTTTT CTTCGTAAAC 
ATATTTGTAG CTCTCATCAT CATCACCTTC CAAGAGCAAG GAGACAAGAT GATGGAGGAC 
TACAGCCTCG AAAAAAATGA GAGAGCCTGT ATTGATTTTG CTATAAATGC CAAACCTCTA 
ACGCGCCACA TGCCTCAGAA CAAGCAAAGC TTTCAGTACC GAATGTGGCA GTTTGTGGTG 
TCGCCTCCGT TTGAGTACAG TATCATGTCT CTCATTGCGC TCAACACCAT CATCCTTATG 
ATGAAGTATA ATGGGGCTTC CGATGCATAT GACAAAGTCC TGAAGAACCT GAATATAGTG 
TTCACCACAT TCTTCTTCAT GGAGAGTATT CTGAAAATCA TTGCCTTTGG TGTCAGAAAT 
TTTTTCAGAG ATGCTTGGAA TATTTTTGAT TTTGTGTCTG TTATCGGCAG CATCACTGAT 
ATTTTAGTGA CTGAGCTCGT GAATAACTTC ATAAACCTGA GTTTTCTGAG ACTCTTTCGA 
GCAGCTCGTC TCATTAAGCT CTTGCGGCAG GGCGAGACCA TTCGCATTCT GCTTTGGACC 
TTCGTTCAGT CATTTAAGGC TCTGCCATAT GTATGCCTGC TCATTGCAAT TCTGTTTTTC 
ATCTACGCCA TCATTGGCAT GCAGCTGTTT GGGAACTTGG CTCTGGATGA CGAAGGTGCT 
ATCAATGAAC ACAACAACTT CAGAACCTTC TTCATGGCCC TCATGCTCCT CTTCAGGAGT 
GCCACGGGCG AGGCGTGGCA TGATATCATG CTGTCATGTC TGGGAGGAAT GCCCTGTGAG 
CCGGAGGAGG TTGGATCAGA ATGTGGCAGC AATGTGGCCT ATGCATACTT CGTCTCTTTC 
ATCTTTCTCT GTTCGTTCCT GATGCTGAAT CTTTTTGTGG CTGTCATCAT GGATAACTTT 
GAGTACTTGA CCAGGGACTC CTCCATCCTG GGTCCGCATC ATTTGGATGA GTATGTCAGG 
ATATGGGCAG AGTACGACCC CGCAGCCTGC GGGCGCATTC ATTATAAGGA TATGTACAGT 
TTATTGCGGG TTATCGACCC CCCGCTGGGC TTAGGCAAGA AATGTCCTCA TAGGGTGGCC 
TGCAAGAGAC TGCTCCGTAT GGACCTGCCC GTTGCTGATG ACAACACGGT GCATTTCAAC 
TCCACTCTGA TGGCCTTGAT TCGCACAGCA CTGGACATCA AAATTGCCAA GGGAGGAGCG 
GACAAACACC AGATGGACGC GGAGCTCCGT AAAGAAATGA TGGCGATTTG GCCAAATCTC 
TCTCAGAAGA ACCTGGATCT GCTGGTAACG CCACACAAGT CAGCGACAGA CCTGACAGTG 
GGCAAGATCT ACGCCGCCAT GATGATCATG GAGTACTACC GGCAGAGCAA GGCCAAAAAG 
CTGCAGGCGC TTCGTGAGGA GCAGAACCGA ACGCCATTAA TGTTTCAGCG CATGGAGCCG 
TCCCCAAACC AGGAAGGGGG ACCGGGGCTG AATGGCCTGC CCGAGCTGCC GCAGGAGCCC 
GTTAACAGCC TGCCTCCAGA GGTTGGAATG AATGAAAGCC AGTCATGGGT GACGTCACGC 
GCCCAGGAGA TGTGCCAGAA GTCCGTTCCA GAAAACTGGA ACCCTGACAG ACCATATCCT 
GATGACCATC ATGACAGCCG CCACAATCCA CAGACCATCA CCGACACAAG TCCAATGCGT 
CGTTCTACAT CCTCATTGGT CCATGGACGT TCAGGTCGAG GCGTGCGATT GGATGATTAC 
TCTCTGGAAC GGGTGGTGTC AGAAGAGGGT CACCGACATG CTCCCCGAAG GAGAGATCGT 
GATCGAGACC GGGATAGAGA TCGCAGTCAT CGAACCTCAG AGAGATCCCT CGCTCGCTAT 
ACAGATGCAG ACACAGGTCT GGGTACAGAT CTTAGCACCA CTACACAGTC GGGTGACCTC 
CCACCTAAAG AAAGGGAACG AGAACGGGGT CGAGCAAAAG ACCGTCGCCA CCACCACCAC 
CATCATCACC ACCACGGCTC GGTGGATAAA GATCGGTACA GTCACGAGCG CGACTACCAT 
CGTCATCCTC AAGACAGATC AGATCGCCAA TGGTCCCGCT CTCCGAGTGA GGGCAGAGGG 
CACAGACAGG GCAGTAGTTC TGTGAGCGGA AGCCCCGTTC CCTCGACGTC GGGGACCAGC 
ACCCCCCGGC GTGGCCGTCG CCAGTTGCCC CAGACCCCAT CCACTCCACG CCCTCACGTC 
ACCTACTCCC CTGCGGTCCG CAAGCCTCCA TATGGCTCCC CAGGCCAGGG TCGACTCCGC 
TCCCCTTCCC CGCGAAACTT TTCTCCGCCA GGTCATGAAC CGCCATACCA CCGTCCACCC 
TCCCGCCATG CCTCACCTCA TCCTGGCTCA CCCCGGCATG CCTCTCCGCG TTCCCCCAGG 
CACACGTCGC CCAGGTCCCC TCGTCACGGC CGCTGGGGCC CTCCGGACAG CCTAGAAGGT 
GATGGAACTT TCTACGAACG GGACTATGAG TACGAGCGCC ACCATGAGCC GCCAGCCTAC 
GAACAGAGTT TATCACAGGG CAACCCCCAT CCACATTCAC ACCCTCATCC CCGGTCACCA 
AGGACTGCTC GTCACGGGCC TCAACCACAC CCGCGGCGTA TGCCTAACGG CTACCGTTCC 
TCCTCGCCCT CCCCGCACCG GCGCGGCCCC CACCCAGGCC CACACCGGCC ACCGCATGGC 
CGTGGGCCCC GGAAGGGGTT GCACGAACAA TATAGCGAGA CGGACGAGGA TGATTGGTGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ab ( XM_021479642.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335317.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X1, mRNA.

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CloneID	ODa147893
Clone ID Related Accession (Same CDS sequence)	XM_021479642.1
Accession Version	XM_021479642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGGCGCAT TCATTATAAG 
GATATGTACA GTTTATTGCG GGTTATCGAC CCCCCGCTGG GCTTAGGCAA GAAATGTCCT 
CATAGGGTGG CCTGCAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCAGCGACA 
GACCTGACAG TGGGCAAGAT CTACGCCGCC ATGATGATCA TGGAGTACTA CCGGCAGAGC 
AAGGCCAAAA AGCTGCAGGC GCTTCGTGAG GAGCAGAACC GAACGCCATT AATGTTTCAG 
CGCATGGAGC CGTCCCCAAA CCAGGAAGGG GGACCGGGGC TGAATGGCCT GCCCGAGCTG 
CCGCAGGAGC CCGTTAACAG CCTGCCTCCA GAGGTTGGAA TGAATGAAAG CCAGTCATGG 
GTGACGTCAC GCGCCCAGGA GATGTGCCAG AAGTCCGTTC CAGAAAACTG GAACCCTGAC 
AGACCATATC CTGATGACCA TCATGACAGC CGCCACAATC CACAGACGGT AGAAATGAGA 
GAGTTTGGGC CTGTTGGGGA CGGTTACTCG GACACTGAGC CCTATCACCC AATGGAAGGG 
CATGGCCGGA CCATCTCCAT GCCTCGCCTC TCTGCAGACA ACCAACGACG GAGACACAGG 
CCGCGAGGAA GTAATCTCAG TACCATCACC GACACAAGTC CAATGCGTCG TTCTACATCC 
TCATTGGTCC ATGGACGTTC AGGTCGAGGC GTGCGATTGG ATGATTACTC TCTGGAACGG 
GTGGTGTCAG AAGAGGGTCA CCGACATGCT CCCCGAAGGA GAGATCGTGA TCGAGACCGG 
GATAGAGATC GCAGTCATCG AACCTCAGAG AGATCCCTCG CTCGCTATAC AGATGCAGAC 
ACAGGTCTGG GTACAGATCT TAGCACCACT ACACAGTCGG GTGACCTCCC ACCTAAAGAA 
AGGGAACGAG AACGGGGTCG AGCAAAAGAC CGTCGCCACC ACCACCACCA TCATCACCAC 
CACGGCTCGG TGGATAAAGA TCGGTACAGT CACGAGCGCG ACTACCATCG TCATCCTCAA 
GACAGATCAG ATCGCCAATG GTCCCGCTCT CCGAGTGAGG GCAGAGGGCA CAGACAGGGC 
AGTAGTTCTG TGAGCGGAAG CCCCGTTCCC TCGACGTCGG GGACCAGCAC CCCCCGGCGT 
GGCCGTCGCC AGTTGCCCCA GACCCCATCC ACTCCACGCC CTCACGTCAC CTACTCCCCT 
GCGGTCCGCA AGCCTCCATA TGGCTCCCCA GGCCAGGGTC GACTCCGCTC CCCTTCCCCG 
CGAAACTTTT CTCCGCCAGG TCATGAACCG CCATACCACC GTCCACCCTC CCGCCATGCC 
TCACCTCATC CTGGCTCACC CCGGCATGCC TCTCCGCGTT CCCCCAGGCA CACGTCGCCC 
AGGTCCCCTC GTCACGGCCG CTGGGGCCCT CCGGACAGCC TAGAAGGTGA TGGAACTTTC 
TACGAACGGG ACTATGAGTA CGAGCGCCAC CATGAGCCGC CAGCCTACGA ACAGAGTTTA 
TCACAGGGCA ACCCCCATCC ACATTCACAC CCTCATCCCC GGTCACCAAG GACTGCTCGT 
CACGGGCCTC AACCACACCC GCGGCGTATG CCTAACGGCT ACCGTTCCTC CTCGCCCTCC 
CCGCACCGGC GCGGCCCCCA CCCAGGCCCA CACCGGCCAC CGCATGGCCG TGGGCCCCGG 
AAGGGGTTGC ACGAACAATA TAGCGAGACG GACGAGGATG ATTGGTGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335317.1
CDS	1436..8446
Translation	MARFGDEVPARYGGGGGGGSGQGGPGRGGSRQGGPPGAQRMYKQSMAQRARTMAIYNPIPVRQNCLTVNRSLFLFSEDNIVRKYAKKITEWPPFEWMILTTIIANCIVLALEQHLPVGDKTPLSERLEETEPYFIAIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSSVGSELDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGVLLFVAILMFAIIGLEFYMGKFHTTCFNTITGEMYDERPCGARTCDANNTQCQGYWLGPNYGITQFDNVLFAVLTVFQCITMEGWTDMLYYSNDALGSAWNWMYYVPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILAEDDNDTGSRRRPTLKKNKADLLNPQEGVDHMGDSVGSPFARGSMSGKGDETPFSKKERRLRFLIRKMVKTQAFYWTVISLVALNTLCVAVVHYDQPELLSDFLYYAEFIFLGLFMSEMLIKMYGLGTRPYFHSSFNCFDCAVIVGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFELGTPPTNFDTFPAAIMTVFQILTGEDWNVVMYDGIKSQGGVDKGMIFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEATTQKMALQKAKEVAEVSPLSAANLSIAAKEQQKNSKGSKSVWEQRTSEIRRQNLMTSREALYNELDAEDHWKMNYRNRPDVKTHLDRPLVVNPQENRNNNTNKPRPDDPNPQERSEEHPSRSHHHHSHHHHHHHHHTHHSSRQSLHLPELAETGFECDVEGAGEHRRPRHHQHRRRDEDGGERGRKHRDHHSRGETAGGDAEDGQEKRQRRHRHANHGDGDGRRERSRLHRNKKEGSLSTARPIQPGLPHTESQYSEDLDNLRNSSKMAIHDPDPDLYDPYYDPEQANNLLNLRGPDLHDSSLILTDSSKLEHTAIDMPPIYPSLNAKLQVNKNANTEPAKEGEKKEEEEDDGGDEEGHKRIPPFSSCFILSTTNPFRRCCHYILTLRYFEMCILSVIAMSSIALAAEDPVSQDSERNKVLRYFDYVFTGVFTFEMLIKMVVLGLILHEGSYFRDLWNFLDFVVVSGALVAFAFTSFSGGSGKGKDISIIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFARDCRGEYLVYERDNEVKSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPDVLKHSVDSTYENRGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYSIMSLIALNTIILMMKYNGASDAYDKVLKNLNIVFTTFFFMESILKIIAFGVRNFFRDAWNIFDFVSVIGSITDILVTELVNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAILFFIYAIIGMQLFGNLALDDEGAINEHNNFRTFFMALMLLFRSATGEAWHDIMLSCLGGMPCEPEEVGSECGSNVAYAYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKKLQALREEQNRTPLMFQRMEPSPNQEGGPGLNGLPELPQEPVNSLPPEVGMNESQSWVTSRAQEMCQKSVPENWNPDRPYPDDHHDSRHNPQTVEMREFGPVGDGYSDTEPYHPMEGHGRTISMPRLSADNQRRRHRPRGSNLSTITDTSPMRRSTSSLVHGRSGRGVRLDDYSLERVVSEEGHRHAPRRRDRDRDRDRDRSHRTSERSLARYTDADTGLGTDLSTTTQSGDLPPKERERERGRAKDRRHHHHHHHHHGSVDKDRYSHERDYHRHPQDRSDRQWSRSPSEGRGHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPSTPRPHVTYSPAVRKPPYGSPGQGRLRSPSPRNFSPPGHEPPYHRPPSRHASPHPGSPRHASPRSPRHTSPRSPRHGRWGPPDSLEGDGTFYERDYEYERHHEPPAYEQSLSQGNPHPHSHPHPRSPRTARHGPQPHPRRMPNGYRSSSPSPHRRGPHPGPHRPPHGRGPRKGLHEQYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021479642.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479642.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGGCGCAT TCATTATAAG 
GATATGTACA GTTTATTGCG GGTTATCGAC CCCCCGCTGG GCTTAGGCAA GAAATGTCCT 
CATAGGGTGG CCTGCAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCAGCGACA 
GACCTGACAG TGGGCAAGAT CTACGCCGCC ATGATGATCA TGGAGTACTA CCGGCAGAGC 
AAGGCCAAAA AGCTGCAGGC GCTTCGTGAG GAGCAGAACC GAACGCCATT AATGTTTCAG 
CGCATGGAGC CGTCCCCAAA CCAGGAAGGG GGACCGGGGC TGAATGGCCT GCCCGAGCTG 
CCGCAGGAGC CCGTTAACAG CCTGCCTCCA GAGGTTGGAA TGAATGAAAG CCAGTCATGG 
GTGACGTCAC GCGCCCAGGA GATGTGCCAG AAGTCCGTTC CAGAAAACTG GAACCCTGAC 
AGACCATATC CTGATGACCA TCATGACAGC CGCCACAATC CACAGACGGT AGAAATGAGA 
GAGTTTGGGC CTGTTGGGGA CGGTTACTCG GACACTGAGC CCTATCACCC AATGGAAGGG 
CATGGCCGGA CCATCTCCAT GCCTCGCCTC TCTGCAGACA ACCAACGACG GAGACACAGG 
CCGCGAGGAA GTAATCTCAG TACCATCACC GACACAAGTC CAATGCGTCG TTCTACATCC 
TCATTGGTCC ATGGACGTTC AGGTCGAGGC GTGCGATTGG ATGATTACTC TCTGGAACGG 
GTGGTGTCAG AAGAGGGTCA CCGACATGCT CCCCGAAGGA GAGATCGTGA TCGAGACCGG 
GATAGAGATC GCAGTCATCG AACCTCAGAG AGATCCCTCG CTCGCTATAC AGATGCAGAC 
ACAGGTCTGG GTACAGATCT TAGCACCACT ACACAGTCGG GTGACCTCCC ACCTAAAGAA 
AGGGAACGAG AACGGGGTCG AGCAAAAGAC CGTCGCCACC ACCACCACCA TCATCACCAC 
CACGGCTCGG TGGATAAAGA TCGGTACAGT CACGAGCGCG ACTACCATCG TCATCCTCAA 
GACAGATCAG ATCGCCAATG GTCCCGCTCT CCGAGTGAGG GCAGAGGGCA CAGACAGGGC 
AGTAGTTCTG TGAGCGGAAG CCCCGTTCCC TCGACGTCGG GGACCAGCAC CCCCCGGCGT 
GGCCGTCGCC AGTTGCCCCA GACCCCATCC ACTCCACGCC CTCACGTCAC CTACTCCCCT 
GCGGTCCGCA AGCCTCCATA TGGCTCCCCA GGCCAGGGTC GACTCCGCTC CCCTTCCCCG 
CGAAACTTTT CTCCGCCAGG TCATGAACCG CCATACCACC GTCCACCCTC CCGCCATGCC 
TCACCTCATC CTGGCTCACC CCGGCATGCC TCTCCGCGTT CCCCCAGGCA CACGTCGCCC 
AGGTCCCCTC GTCACGGCCG CTGGGGCCCT CCGGACAGCC TAGAAGGTGA TGGAACTTTC 
TACGAACGGG ACTATGAGTA CGAGCGCCAC CATGAGCCGC CAGCCTACGA ACAGAGTTTA 
TCACAGGGCA ACCCCCATCC ACATTCACAC CCTCATCCCC GGTCACCAAG GACTGCTCGT 
CACGGGCCTC AACCACACCC GCGGCGTATG CCTAACGGCT ACCGTTCCTC CTCGCCCTCC 
CCGCACCGGC GCGGCCCCCA CCCAGGCCCA CACCGGCCAC CGCATGGCCG TGGGCCCCGG 
AAGGGGTTGC ACGAACAATA TAGCGAGACG GACGAGGATG ATTGGTGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ab ( XM_021479643.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335318.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X2, mRNA.

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CloneID	ODa147894
Clone ID Related Accession (Same CDS sequence)	XM_021479643.1
Accession Version	XM_021479643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGCAGGAT CAGTTACAGT 
GATATGTATG AGATGCTCAG GCACATGTGT CCTCCGCTAG GCCTGGGGAA GAGGTGCCCT 
GCTCGGGTCG CCTACAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCAGCGACA 
GACCTGACAG TGGGCAAGAT CTACGCCGCC ATGATGATCA TGGAGTACTA CCGGCAGAGC 
AAGGCCAAAA AGCTGCAGGC GCTTCGTGAG GAGCAGAACC GAACGCCATT AATGTTTCAG 
CGCATGGAGC CGTCCCCAAA CCAGGAAGGG GGACCGGGGC TGAATGGCCT GCCCGAGCTG 
CCGCAGGAGC CCGTTAACAG CCTGCCTCCA GAGGTTGGAA TGAATGAAAG CCAGTCATGG 
GTGACGTCAC GCGCCCAGGA GATGTGCCAG AAGTCCGTTC CAGAAAACTG GAACCCTGAC 
AGACCATATC CTGATGACCA TCATGACAGC CGCCACAATC CACAGACGGT AGAAATGAGA 
GAGTTTGGGC CTGTTGGGGA CGGTTACTCG GACACTGAGC CCTATCACCC AATGGAAGGG 
CATGGCCGGA CCATCTCCAT GCCTCGCCTC TCTGCAGACA ACCAACGACG GAGACACAGG 
CCGCGAGGAA GTAATCTCAG TACCATCACC GACACAAGTC CAATGCGTCG TTCTACATCC 
TCATTGGTCC ATGGACGTTC AGGTCGAGGC GTGCGATTGG ATGATTACTC TCTGGAACGG 
GTGGTGTCAG AAGAGGGTCA CCGACATGCT CCCCGAAGGA GAGATCGTGA TCGAGACCGG 
GATAGAGATC GCAGTCATCG AACCTCAGAG AGATCCCTCG CTCGCTATAC AGATGCAGAC 
ACAGGTCTGG GTACAGATCT TAGCACCACT ACACAGTCGG GTGACCTCCC ACCTAAAGAA 
AGGGAACGAG AACGGGGTCG AGCAAAAGAC CGTCGCCACC ACCACCACCA TCATCACCAC 
CACGGCTCGG TGGATAAAGA TCGGTACAGT CACGAGCGCG ACTACCATCG TCATCCTCAA 
GACAGATCAG ATCGCCAATG GTCCCGCTCT CCGAGTGAGG GCAGAGGGCA CAGACAGGGC 
AGTAGTTCTG TGAGCGGAAG CCCCGTTCCC TCGACGTCGG GGACCAGCAC CCCCCGGCGT 
GGCCGTCGCC AGTTGCCCCA GACCCCATCC ACTCCACGCC CTCACGTCAC CTACTCCCCT 
GCGGTCCGCA AGCCTCCATA TGGCTCCCCA GGCCAGGGTC GACTCCGCTC CCCTTCCCCG 
CGAAACTTTT CTCCGCCAGG TCATGAACCG CCATACCACC GTCCACCCTC CCGCCATGCC 
TCACCTCATC CTGGCTCACC CCGGCATGCC TCTCCGCGTT CCCCCAGGCA CACGTCGCCC 
AGGTCCCCTC GTCACGGCCG CTGGGGCCCT CCGGACAGCC TAGAAGGTGA TGGAACTTTC 
TACGAACGGG ACTATGAGTA CGAGCGCCAC CATGAGCCGC CAGCCTACGA ACAGAGTTTA 
TCACAGGGCA ACCCCCATCC ACATTCACAC CCTCATCCCC GGTCACCAAG GACTGCTCGT 
CACGGGCCTC AACCACACCC GCGGCGTATG CCTAACGGCT ACCGTTCCTC CTCGCCCTCC 
CCGCACCGGC GCGGCCCCCA CCCAGGCCCA CACCGGCCAC CGCATGGCCG TGGGCCCCGG 
AAGGGGTTGC ACGAACAATA TAGCGAGACG GACGAGGATG ATTGGTGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335318.1
CDS	1436..8446
Translation	MARFGDEVPARYGGGGGGGSGQGGPGRGGSRQGGPPGAQRMYKQSMAQRARTMAIYNPIPVRQNCLTVNRSLFLFSEDNIVRKYAKKITEWPPFEWMILTTIIANCIVLALEQHLPVGDKTPLSERLEETEPYFIAIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSSVGSELDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGVLLFVAILMFAIIGLEFYMGKFHTTCFNTITGEMYDERPCGARTCDANNTQCQGYWLGPNYGITQFDNVLFAVLTVFQCITMEGWTDMLYYSNDALGSAWNWMYYVPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILAEDDNDTGSRRRPTLKKNKADLLNPQEGVDHMGDSVGSPFARGSMSGKGDETPFSKKERRLRFLIRKMVKTQAFYWTVISLVALNTLCVAVVHYDQPELLSDFLYYAEFIFLGLFMSEMLIKMYGLGTRPYFHSSFNCFDCAVIVGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFELGTPPTNFDTFPAAIMTVFQILTGEDWNVVMYDGIKSQGGVDKGMIFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEATTQKMALQKAKEVAEVSPLSAANLSIAAKEQQKNSKGSKSVWEQRTSEIRRQNLMTSREALYNELDAEDHWKMNYRNRPDVKTHLDRPLVVNPQENRNNNTNKPRPDDPNPQERSEEHPSRSHHHHSHHHHHHHHHTHHSSRQSLHLPELAETGFECDVEGAGEHRRPRHHQHRRRDEDGGERGRKHRDHHSRGETAGGDAEDGQEKRQRRHRHANHGDGDGRRERSRLHRNKKEGSLSTARPIQPGLPHTESQYSEDLDNLRNSSKMAIHDPDPDLYDPYYDPEQANNLLNLRGPDLHDSSLILTDSSKLEHTAIDMPPIYPSLNAKLQVNKNANTEPAKEGEKKEEEEDDGGDEEGHKRIPPFSSCFILSTTNPFRRCCHYILTLRYFEMCILSVIAMSSIALAAEDPVSQDSERNKVLRYFDYVFTGVFTFEMLIKMVVLGLILHEGSYFRDLWNFLDFVVVSGALVAFAFTSFSGGSGKGKDISIIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFARDCRGEYLVYERDNEVKSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPDVLKHSVDSTYENRGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYSIMSLIALNTIILMMKYNGASDAYDKVLKNLNIVFTTFFFMESILKIIAFGVRNFFRDAWNIFDFVSVIGSITDILVTELVNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAILFFIYAIIGMQLFGNLALDDEGAINEHNNFRTFFMALMLLFRSATGEAWHDIMLSCLGGMPCEPEEVGSECGSNVAYAYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRISYSDMYEMLRHMCPPLGLGKRCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKKLQALREEQNRTPLMFQRMEPSPNQEGGPGLNGLPELPQEPVNSLPPEVGMNESQSWVTSRAQEMCQKSVPENWNPDRPYPDDHHDSRHNPQTVEMREFGPVGDGYSDTEPYHPMEGHGRTISMPRLSADNQRRRHRPRGSNLSTITDTSPMRRSTSSLVHGRSGRGVRLDDYSLERVVSEEGHRHAPRRRDRDRDRDRDRSHRTSERSLARYTDADTGLGTDLSTTTQSGDLPPKERERERGRAKDRRHHHHHHHHHGSVDKDRYSHERDYHRHPQDRSDRQWSRSPSEGRGHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPSTPRPHVTYSPAVRKPPYGSPGQGRLRSPSPRNFSPPGHEPPYHRPPSRHASPHPGSPRHASPRSPRHTSPRSPRHGRWGPPDSLEGDGTFYERDYEYERHHEPPAYEQSLSQGNPHPHSHPHPRSPRTARHGPQPHPRRMPNGYRSSSPSPHRRGPHPGPHRPPHGRGPRKGLHEQYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021479643.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479643.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGCAGGAT CAGTTACAGT 
GATATGTATG AGATGCTCAG GCACATGTGT CCTCCGCTAG GCCTGGGGAA GAGGTGCCCT 
GCTCGGGTCG CCTACAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCAGCGACA 
GACCTGACAG TGGGCAAGAT CTACGCCGCC ATGATGATCA TGGAGTACTA CCGGCAGAGC 
AAGGCCAAAA AGCTGCAGGC GCTTCGTGAG GAGCAGAACC GAACGCCATT AATGTTTCAG 
CGCATGGAGC CGTCCCCAAA CCAGGAAGGG GGACCGGGGC TGAATGGCCT GCCCGAGCTG 
CCGCAGGAGC CCGTTAACAG CCTGCCTCCA GAGGTTGGAA TGAATGAAAG CCAGTCATGG 
GTGACGTCAC GCGCCCAGGA GATGTGCCAG AAGTCCGTTC CAGAAAACTG GAACCCTGAC 
AGACCATATC CTGATGACCA TCATGACAGC CGCCACAATC CACAGACGGT AGAAATGAGA 
GAGTTTGGGC CTGTTGGGGA CGGTTACTCG GACACTGAGC CCTATCACCC AATGGAAGGG 
CATGGCCGGA CCATCTCCAT GCCTCGCCTC TCTGCAGACA ACCAACGACG GAGACACAGG 
CCGCGAGGAA GTAATCTCAG TACCATCACC GACACAAGTC CAATGCGTCG TTCTACATCC 
TCATTGGTCC ATGGACGTTC AGGTCGAGGC GTGCGATTGG ATGATTACTC TCTGGAACGG 
GTGGTGTCAG AAGAGGGTCA CCGACATGCT CCCCGAAGGA GAGATCGTGA TCGAGACCGG 
GATAGAGATC GCAGTCATCG AACCTCAGAG AGATCCCTCG CTCGCTATAC AGATGCAGAC 
ACAGGTCTGG GTACAGATCT TAGCACCACT ACACAGTCGG GTGACCTCCC ACCTAAAGAA 
AGGGAACGAG AACGGGGTCG AGCAAAAGAC CGTCGCCACC ACCACCACCA TCATCACCAC 
CACGGCTCGG TGGATAAAGA TCGGTACAGT CACGAGCGCG ACTACCATCG TCATCCTCAA 
GACAGATCAG ATCGCCAATG GTCCCGCTCT CCGAGTGAGG GCAGAGGGCA CAGACAGGGC 
AGTAGTTCTG TGAGCGGAAG CCCCGTTCCC TCGACGTCGG GGACCAGCAC CCCCCGGCGT 
GGCCGTCGCC AGTTGCCCCA GACCCCATCC ACTCCACGCC CTCACGTCAC CTACTCCCCT 
GCGGTCCGCA AGCCTCCATA TGGCTCCCCA GGCCAGGGTC GACTCCGCTC CCCTTCCCCG 
CGAAACTTTT CTCCGCCAGG TCATGAACCG CCATACCACC GTCCACCCTC CCGCCATGCC 
TCACCTCATC CTGGCTCACC CCGGCATGCC TCTCCGCGTT CCCCCAGGCA CACGTCGCCC 
AGGTCCCCTC GTCACGGCCG CTGGGGCCCT CCGGACAGCC TAGAAGGTGA TGGAACTTTC 
TACGAACGGG ACTATGAGTA CGAGCGCCAC CATGAGCCGC CAGCCTACGA ACAGAGTTTA 
TCACAGGGCA ACCCCCATCC ACATTCACAC CCTCATCCCC GGTCACCAAG GACTGCTCGT 
CACGGGCCTC AACCACACCC GCGGCGTATG CCTAACGGCT ACCGTTCCTC CTCGCCCTCC 
CCGCACCGGC GCGGCCCCCA CCCAGGCCCA CACCGGCCAC CGCATGGCCG TGGGCCCCGG 
AAGGGGTTGC ACGAACAATA TAGCGAGACG GACGAGGATG ATTGGTGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ab ( XM_021479644.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335319.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X3, mRNA.

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CloneID	ODa147895
Clone ID Related Accession (Same CDS sequence)	XM_021479644.1
Accession Version	XM_021479644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGGCGCAT TCATTATAAG 
GATATGTACA GTTTATTGCG GGTTATCGAC CCCCCGCTGG GCTTAGGCAA GAAATGTCCT 
CATAGGGTGG CCTGCAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCGACAGAC 
CTGACAGTGG GCAAGATCTA CGCCGCCATG ATGATCATGG AGTACTACCG GCAGAGCAAG 
GCCAAAAAGC TGCAGGCGCT TCGTGAGGAG CAGAACCGAA CGCCATTAAT GTTTCAGCGC 
ATGGAGCCGT CCCCAAACCA GGAAGGGGGA CCGGGGCTGA ATGGCCTGCC CGAGCTGCCG 
CAGGAGCCCG TTAACAGCCT GCCTCCAGAG GTTGGAATGA ATGAAAGCCA GTCATGGGTG 
ACGTCACGCG CCCAGGAGAT GTGCCAGAAG TCCGTTCCAG AAAACTGGAA CCCTGACAGA 
CCATATCCTG ATGACCATCA TGACAGCCGC CACAATCCAC AGACGGTAGA AATGAGAGAG 
TTTGGGCCTG TTGGGGACGG TTACTCGGAC ACTGAGCCCT ATCACCCAAT GGAAGGGCAT 
GGCCGGACCA TCTCCATGCC TCGCCTCTCT GCAGACAACC AACGACGGAG ACACAGGCCG 
CGAGGAAGTA ATCTCAGTAC CATCACCGAC ACAAGTCCAA TGCGTCGTTC TACATCCTCA 
TTGGTCCATG GACGTTCAGG TCGAGGCGTG CGATTGGATG ATTACTCTCT GGAACGGGTG 
GTGTCAGAAG AGGGTCACCG ACATGCTCCC CGAAGGAGAG ATCGTGATCG AGACCGGGAT 
AGAGATCGCA GTCATCGAAC CTCAGAGAGA TCCCTCGCTC GCTATACAGA TGCAGACACA 
GGTCTGGGTA CAGATCTTAG CACCACTACA CAGTCGGGTG ACCTCCCACC TAAAGAAAGG 
GAACGAGAAC GGGGTCGAGC AAAAGACCGT CGCCACCACC ACCACCATCA TCACCACCAC 
GGCTCGGTGG ATAAAGATCG GTACAGTCAC GAGCGCGACT ACCATCGTCA TCCTCAAGAC 
AGATCAGATC GCCAATGGTC CCGCTCTCCG AGTGAGGGCA GAGGGCACAG ACAGGGCAGT 
AGTTCTGTGA GCGGAAGCCC CGTTCCCTCG ACGTCGGGGA CCAGCACCCC CCGGCGTGGC 
CGTCGCCAGT TGCCCCAGAC CCCATCCACT CCACGCCCTC ACGTCACCTA CTCCCCTGCG 
GTCCGCAAGC CTCCATATGG CTCCCCAGGC CAGGGTCGAC TCCGCTCCCC TTCCCCGCGA 
AACTTTTCTC CGCCAGGTCA TGAACCGCCA TACCACCGTC CACCCTCCCG CCATGCCTCA 
CCTCATCCTG GCTCACCCCG GCATGCCTCT CCGCGTTCCC CCAGGCACAC GTCGCCCAGG 
TCCCCTCGTC ACGGCCGCTG GGGCCCTCCG GACAGCCTAG AAGGTGATGG AACTTTCTAC 
GAACGGGACT ATGAGTACGA GCGCCACCAT GAGCCGCCAG CCTACGAACA GAGTTTATCA 
CAGGGCAACC CCCATCCACA TTCACACCCT CATCCCCGGT CACCAAGGAC TGCTCGTCAC 
GGGCCTCAAC CACACCCGCG GCGTATGCCT AACGGCTACC GTTCCTCCTC GCCCTCCCCG 
CACCGGCGCG GCCCCCACCC AGGCCCACAC CGGCCACCGC ATGGCCGTGG GCCCCGGAAG 
GGGTTGCACG AACAATATAG CGAGACGGAC GAGGATGATT GGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335319.1
CDS	1436..8443
Translation	MARFGDEVPARYGGGGGGGSGQGGPGRGGSRQGGPPGAQRMYKQSMAQRARTMAIYNPIPVRQNCLTVNRSLFLFSEDNIVRKYAKKITEWPPFEWMILTTIIANCIVLALEQHLPVGDKTPLSERLEETEPYFIAIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSSVGSELDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGVLLFVAILMFAIIGLEFYMGKFHTTCFNTITGEMYDERPCGARTCDANNTQCQGYWLGPNYGITQFDNVLFAVLTVFQCITMEGWTDMLYYSNDALGSAWNWMYYVPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILAEDDNDTGSRRRPTLKKNKADLLNPQEGVDHMGDSVGSPFARGSMSGKGDETPFSKKERRLRFLIRKMVKTQAFYWTVISLVALNTLCVAVVHYDQPELLSDFLYYAEFIFLGLFMSEMLIKMYGLGTRPYFHSSFNCFDCAVIVGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFELGTPPTNFDTFPAAIMTVFQILTGEDWNVVMYDGIKSQGGVDKGMIFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEATTQKMALQKAKEVAEVSPLSAANLSIAAKEQQKNSKGSKSVWEQRTSEIRRQNLMTSREALYNELDAEDHWKMNYRNRPDVKTHLDRPLVVNPQENRNNNTNKPRPDDPNPQERSEEHPSRSHHHHSHHHHHHHHHTHHSSRQSLHLPELAETGFECDVEGAGEHRRPRHHQHRRRDEDGGERGRKHRDHHSRGETAGGDAEDGQEKRQRRHRHANHGDGDGRRERSRLHRNKKEGSLSTARPIQPGLPHTESQYSEDLDNLRNSSKMAIHDPDPDLYDPYYDPEQANNLLNLRGPDLHDSSLILTDSSKLEHTAIDMPPIYPSLNAKLQVNKNANTEPAKEGEKKEEEEDDGGDEEGHKRIPPFSSCFILSTTNPFRRCCHYILTLRYFEMCILSVIAMSSIALAAEDPVSQDSERNKVLRYFDYVFTGVFTFEMLIKMVVLGLILHEGSYFRDLWNFLDFVVVSGALVAFAFTSFSGGSGKGKDISIIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFARDCRGEYLVYERDNEVKSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPDVLKHSVDSTYENRGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYSIMSLIALNTIILMMKYNGASDAYDKVLKNLNIVFTTFFFMESILKIIAFGVRNFFRDAWNIFDFVSVIGSITDILVTELVNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAILFFIYAIIGMQLFGNLALDDEGAINEHNNFRTFFMALMLLFRSATGEAWHDIMLSCLGGMPCEPEEVGSECGSNVAYAYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQALREEQNRTPLMFQRMEPSPNQEGGPGLNGLPELPQEPVNSLPPEVGMNESQSWVTSRAQEMCQKSVPENWNPDRPYPDDHHDSRHNPQTVEMREFGPVGDGYSDTEPYHPMEGHGRTISMPRLSADNQRRRHRPRGSNLSTITDTSPMRRSTSSLVHGRSGRGVRLDDYSLERVVSEEGHRHAPRRRDRDRDRDRDRSHRTSERSLARYTDADTGLGTDLSTTTQSGDLPPKERERERGRAKDRRHHHHHHHHHGSVDKDRYSHERDYHRHPQDRSDRQWSRSPSEGRGHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPSTPRPHVTYSPAVRKPPYGSPGQGRLRSPSPRNFSPPGHEPPYHRPPSRHASPHPGSPRHASPRSPRHTSPRSPRHGRWGPPDSLEGDGTFYERDYEYERHHEPPAYEQSLSQGNPHPHSHPHPRSPRTARHGPQPHPRRMPNGYRSSSPSPHRRGPHPGPHRPPHGRGPRKGLHEQYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021479644.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479644.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGGCGCAT TCATTATAAG 
GATATGTACA GTTTATTGCG GGTTATCGAC CCCCCGCTGG GCTTAGGCAA GAAATGTCCT 
CATAGGGTGG CCTGCAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCGACAGAC 
CTGACAGTGG GCAAGATCTA CGCCGCCATG ATGATCATGG AGTACTACCG GCAGAGCAAG 
GCCAAAAAGC TGCAGGCGCT TCGTGAGGAG CAGAACCGAA CGCCATTAAT GTTTCAGCGC 
ATGGAGCCGT CCCCAAACCA GGAAGGGGGA CCGGGGCTGA ATGGCCTGCC CGAGCTGCCG 
CAGGAGCCCG TTAACAGCCT GCCTCCAGAG GTTGGAATGA ATGAAAGCCA GTCATGGGTG 
ACGTCACGCG CCCAGGAGAT GTGCCAGAAG TCCGTTCCAG AAAACTGGAA CCCTGACAGA 
CCATATCCTG ATGACCATCA TGACAGCCGC CACAATCCAC AGACGGTAGA AATGAGAGAG 
TTTGGGCCTG TTGGGGACGG TTACTCGGAC ACTGAGCCCT ATCACCCAAT GGAAGGGCAT 
GGCCGGACCA TCTCCATGCC TCGCCTCTCT GCAGACAACC AACGACGGAG ACACAGGCCG 
CGAGGAAGTA ATCTCAGTAC CATCACCGAC ACAAGTCCAA TGCGTCGTTC TACATCCTCA 
TTGGTCCATG GACGTTCAGG TCGAGGCGTG CGATTGGATG ATTACTCTCT GGAACGGGTG 
GTGTCAGAAG AGGGTCACCG ACATGCTCCC CGAAGGAGAG ATCGTGATCG AGACCGGGAT 
AGAGATCGCA GTCATCGAAC CTCAGAGAGA TCCCTCGCTC GCTATACAGA TGCAGACACA 
GGTCTGGGTA CAGATCTTAG CACCACTACA CAGTCGGGTG ACCTCCCACC TAAAGAAAGG 
GAACGAGAAC GGGGTCGAGC AAAAGACCGT CGCCACCACC ACCACCATCA TCACCACCAC 
GGCTCGGTGG ATAAAGATCG GTACAGTCAC GAGCGCGACT ACCATCGTCA TCCTCAAGAC 
AGATCAGATC GCCAATGGTC CCGCTCTCCG AGTGAGGGCA GAGGGCACAG ACAGGGCAGT 
AGTTCTGTGA GCGGAAGCCC CGTTCCCTCG ACGTCGGGGA CCAGCACCCC CCGGCGTGGC 
CGTCGCCAGT TGCCCCAGAC CCCATCCACT CCACGCCCTC ACGTCACCTA CTCCCCTGCG 
GTCCGCAAGC CTCCATATGG CTCCCCAGGC CAGGGTCGAC TCCGCTCCCC TTCCCCGCGA 
AACTTTTCTC CGCCAGGTCA TGAACCGCCA TACCACCGTC CACCCTCCCG CCATGCCTCA 
CCTCATCCTG GCTCACCCCG GCATGCCTCT CCGCGTTCCC CCAGGCACAC GTCGCCCAGG 
TCCCCTCGTC ACGGCCGCTG GGGCCCTCCG GACAGCCTAG AAGGTGATGG AACTTTCTAC 
GAACGGGACT ATGAGTACGA GCGCCACCAT GAGCCGCCAG CCTACGAACA GAGTTTATCA 
CAGGGCAACC CCCATCCACA TTCACACCCT CATCCCCGGT CACCAAGGAC TGCTCGTCAC 
GGGCCTCAAC CACACCCGCG GCGTATGCCT AACGGCTACC GTTCCTCCTC GCCCTCCCCG 
CACCGGCGCG GCCCCCACCC AGGCCCACAC CGGCCACCGC ATGGCCGTGG GCCCCGGAAG 
GGGTTGCACG AACAATATAG CGAGACGGAC GAGGATGATT GGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ab ( XM_021479645.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335320.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X4, mRNA.

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CloneID	ODa147896
Clone ID Related Accession (Same CDS sequence)	XM_021479645.1
Accession Version	XM_021479645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGCAGGAT CAGTTACAGT 
GATATGTATG AGATGCTCAG GCACATGTGT CCTCCGCTAG GCCTGGGGAA GAGGTGCCCT 
GCTCGGGTCG CCTACAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCGACAGAC 
CTGACAGTGG GCAAGATCTA CGCCGCCATG ATGATCATGG AGTACTACCG GCAGAGCAAG 
GCCAAAAAGC TGCAGGCGCT TCGTGAGGAG CAGAACCGAA CGCCATTAAT GTTTCAGCGC 
ATGGAGCCGT CCCCAAACCA GGAAGGGGGA CCGGGGCTGA ATGGCCTGCC CGAGCTGCCG 
CAGGAGCCCG TTAACAGCCT GCCTCCAGAG GTTGGAATGA ATGAAAGCCA GTCATGGGTG 
ACGTCACGCG CCCAGGAGAT GTGCCAGAAG TCCGTTCCAG AAAACTGGAA CCCTGACAGA 
CCATATCCTG ATGACCATCA TGACAGCCGC CACAATCCAC AGACGGTAGA AATGAGAGAG 
TTTGGGCCTG TTGGGGACGG TTACTCGGAC ACTGAGCCCT ATCACCCAAT GGAAGGGCAT 
GGCCGGACCA TCTCCATGCC TCGCCTCTCT GCAGACAACC AACGACGGAG ACACAGGCCG 
CGAGGAAGTA ATCTCAGTAC CATCACCGAC ACAAGTCCAA TGCGTCGTTC TACATCCTCA 
TTGGTCCATG GACGTTCAGG TCGAGGCGTG CGATTGGATG ATTACTCTCT GGAACGGGTG 
GTGTCAGAAG AGGGTCACCG ACATGCTCCC CGAAGGAGAG ATCGTGATCG AGACCGGGAT 
AGAGATCGCA GTCATCGAAC CTCAGAGAGA TCCCTCGCTC GCTATACAGA TGCAGACACA 
GGTCTGGGTA CAGATCTTAG CACCACTACA CAGTCGGGTG ACCTCCCACC TAAAGAAAGG 
GAACGAGAAC GGGGTCGAGC AAAAGACCGT CGCCACCACC ACCACCATCA TCACCACCAC 
GGCTCGGTGG ATAAAGATCG GTACAGTCAC GAGCGCGACT ACCATCGTCA TCCTCAAGAC 
AGATCAGATC GCCAATGGTC CCGCTCTCCG AGTGAGGGCA GAGGGCACAG ACAGGGCAGT 
AGTTCTGTGA GCGGAAGCCC CGTTCCCTCG ACGTCGGGGA CCAGCACCCC CCGGCGTGGC 
CGTCGCCAGT TGCCCCAGAC CCCATCCACT CCACGCCCTC ACGTCACCTA CTCCCCTGCG 
GTCCGCAAGC CTCCATATGG CTCCCCAGGC CAGGGTCGAC TCCGCTCCCC TTCCCCGCGA 
AACTTTTCTC CGCCAGGTCA TGAACCGCCA TACCACCGTC CACCCTCCCG CCATGCCTCA 
CCTCATCCTG GCTCACCCCG GCATGCCTCT CCGCGTTCCC CCAGGCACAC GTCGCCCAGG 
TCCCCTCGTC ACGGCCGCTG GGGCCCTCCG GACAGCCTAG AAGGTGATGG AACTTTCTAC 
GAACGGGACT ATGAGTACGA GCGCCACCAT GAGCCGCCAG CCTACGAACA GAGTTTATCA 
CAGGGCAACC CCCATCCACA TTCACACCCT CATCCCCGGT CACCAAGGAC TGCTCGTCAC 
GGGCCTCAAC CACACCCGCG GCGTATGCCT AACGGCTACC GTTCCTCCTC GCCCTCCCCG 
CACCGGCGCG GCCCCCACCC AGGCCCACAC CGGCCACCGC ATGGCCGTGG GCCCCGGAAG 
GGGTTGCACG AACAATATAG CGAGACGGAC GAGGATGATT GGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335320.1
CDS	1436..8443
Translation	MARFGDEVPARYGGGGGGGSGQGGPGRGGSRQGGPPGAQRMYKQSMAQRARTMAIYNPIPVRQNCLTVNRSLFLFSEDNIVRKYAKKITEWPPFEWMILTTIIANCIVLALEQHLPVGDKTPLSERLEETEPYFIAIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSSVGSELDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGVLLFVAILMFAIIGLEFYMGKFHTTCFNTITGEMYDERPCGARTCDANNTQCQGYWLGPNYGITQFDNVLFAVLTVFQCITMEGWTDMLYYSNDALGSAWNWMYYVPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILAEDDNDTGSRRRPTLKKNKADLLNPQEGVDHMGDSVGSPFARGSMSGKGDETPFSKKERRLRFLIRKMVKTQAFYWTVISLVALNTLCVAVVHYDQPELLSDFLYYAEFIFLGLFMSEMLIKMYGLGTRPYFHSSFNCFDCAVIVGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFELGTPPTNFDTFPAAIMTVFQILTGEDWNVVMYDGIKSQGGVDKGMIFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEATTQKMALQKAKEVAEVSPLSAANLSIAAKEQQKNSKGSKSVWEQRTSEIRRQNLMTSREALYNELDAEDHWKMNYRNRPDVKTHLDRPLVVNPQENRNNNTNKPRPDDPNPQERSEEHPSRSHHHHSHHHHHHHHHTHHSSRQSLHLPELAETGFECDVEGAGEHRRPRHHQHRRRDEDGGERGRKHRDHHSRGETAGGDAEDGQEKRQRRHRHANHGDGDGRRERSRLHRNKKEGSLSTARPIQPGLPHTESQYSEDLDNLRNSSKMAIHDPDPDLYDPYYDPEQANNLLNLRGPDLHDSSLILTDSSKLEHTAIDMPPIYPSLNAKLQVNKNANTEPAKEGEKKEEEEDDGGDEEGHKRIPPFSSCFILSTTNPFRRCCHYILTLRYFEMCILSVIAMSSIALAAEDPVSQDSERNKVLRYFDYVFTGVFTFEMLIKMVVLGLILHEGSYFRDLWNFLDFVVVSGALVAFAFTSFSGGSGKGKDISIIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFARDCRGEYLVYERDNEVKSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPDVLKHSVDSTYENRGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYSIMSLIALNTIILMMKYNGASDAYDKVLKNLNIVFTTFFFMESILKIIAFGVRNFFRDAWNIFDFVSVIGSITDILVTELVNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAILFFIYAIIGMQLFGNLALDDEGAINEHNNFRTFFMALMLLFRSATGEAWHDIMLSCLGGMPCEPEEVGSECGSNVAYAYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRISYSDMYEMLRHMCPPLGLGKRCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQALREEQNRTPLMFQRMEPSPNQEGGPGLNGLPELPQEPVNSLPPEVGMNESQSWVTSRAQEMCQKSVPENWNPDRPYPDDHHDSRHNPQTVEMREFGPVGDGYSDTEPYHPMEGHGRTISMPRLSADNQRRRHRPRGSNLSTITDTSPMRRSTSSLVHGRSGRGVRLDDYSLERVVSEEGHRHAPRRRDRDRDRDRDRSHRTSERSLARYTDADTGLGTDLSTTTQSGDLPPKERERERGRAKDRRHHHHHHHHHGSVDKDRYSHERDYHRHPQDRSDRQWSRSPSEGRGHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPSTPRPHVTYSPAVRKPPYGSPGQGRLRSPSPRNFSPPGHEPPYHRPPSRHASPHPGSPRHASPRSPRHTSPRSPRHGRWGPPDSLEGDGTFYERDYEYERHHEPPAYEQSLSQGNPHPHSHPHPRSPRTARHGPQPHPRRMPNGYRSSSPSPHRRGPHPGPHRPPHGRGPRKGLHEQYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021479645.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479645.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGCAGGAT CAGTTACAGT 
GATATGTATG AGATGCTCAG GCACATGTGT CCTCCGCTAG GCCTGGGGAA GAGGTGCCCT 
GCTCGGGTCG CCTACAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCGACAGAC 
CTGACAGTGG GCAAGATCTA CGCCGCCATG ATGATCATGG AGTACTACCG GCAGAGCAAG 
GCCAAAAAGC TGCAGGCGCT TCGTGAGGAG CAGAACCGAA CGCCATTAAT GTTTCAGCGC 
ATGGAGCCGT CCCCAAACCA GGAAGGGGGA CCGGGGCTGA ATGGCCTGCC CGAGCTGCCG 
CAGGAGCCCG TTAACAGCCT GCCTCCAGAG GTTGGAATGA ATGAAAGCCA GTCATGGGTG 
ACGTCACGCG CCCAGGAGAT GTGCCAGAAG TCCGTTCCAG AAAACTGGAA CCCTGACAGA 
CCATATCCTG ATGACCATCA TGACAGCCGC CACAATCCAC AGACGGTAGA AATGAGAGAG 
TTTGGGCCTG TTGGGGACGG TTACTCGGAC ACTGAGCCCT ATCACCCAAT GGAAGGGCAT 
GGCCGGACCA TCTCCATGCC TCGCCTCTCT GCAGACAACC AACGACGGAG ACACAGGCCG 
CGAGGAAGTA ATCTCAGTAC CATCACCGAC ACAAGTCCAA TGCGTCGTTC TACATCCTCA 
TTGGTCCATG GACGTTCAGG TCGAGGCGTG CGATTGGATG ATTACTCTCT GGAACGGGTG 
GTGTCAGAAG AGGGTCACCG ACATGCTCCC CGAAGGAGAG ATCGTGATCG AGACCGGGAT 
AGAGATCGCA GTCATCGAAC CTCAGAGAGA TCCCTCGCTC GCTATACAGA TGCAGACACA 
GGTCTGGGTA CAGATCTTAG CACCACTACA CAGTCGGGTG ACCTCCCACC TAAAGAAAGG 
GAACGAGAAC GGGGTCGAGC AAAAGACCGT CGCCACCACC ACCACCATCA TCACCACCAC 
GGCTCGGTGG ATAAAGATCG GTACAGTCAC GAGCGCGACT ACCATCGTCA TCCTCAAGAC 
AGATCAGATC GCCAATGGTC CCGCTCTCCG AGTGAGGGCA GAGGGCACAG ACAGGGCAGT 
AGTTCTGTGA GCGGAAGCCC CGTTCCCTCG ACGTCGGGGA CCAGCACCCC CCGGCGTGGC 
CGTCGCCAGT TGCCCCAGAC CCCATCCACT CCACGCCCTC ACGTCACCTA CTCCCCTGCG 
GTCCGCAAGC CTCCATATGG CTCCCCAGGC CAGGGTCGAC TCCGCTCCCC TTCCCCGCGA 
AACTTTTCTC CGCCAGGTCA TGAACCGCCA TACCACCGTC CACCCTCCCG CCATGCCTCA 
CCTCATCCTG GCTCACCCCG GCATGCCTCT CCGCGTTCCC CCAGGCACAC GTCGCCCAGG 
TCCCCTCGTC ACGGCCGCTG GGGCCCTCCG GACAGCCTAG AAGGTGATGG AACTTTCTAC 
GAACGGGACT ATGAGTACGA GCGCCACCAT GAGCCGCCAG CCTACGAACA GAGTTTATCA 
CAGGGCAACC CCCATCCACA TTCACACCCT CATCCCCGGT CACCAAGGAC TGCTCGTCAC 
GGGCCTCAAC CACACCCGCG GCGTATGCCT AACGGCTACC GTTCCTCCTC GCCCTCCCCG 
CACCGGCGCG GCCCCCACCC AGGCCCACAC CGGCCACCGC ATGGCCGTGG GCCCCGGAAG 
GGGTTGCACG AACAATATAG CGAGACGGAC GAGGATGATT GGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ab ( XM_021479646.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335321.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X5, mRNA.

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CloneID	ODa147897
Clone ID Related Accession (Same CDS sequence)	XM_021479646.1
Accession Version	XM_021479646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAGGTC ATACTCGCAG AGGATGACAA TGACACCGGA 
TCTCGACGAA GACCCACATT AAAGAAAAAT AAAGCTGACC TGTTAAATCC ACAAGAGGGA 
GTGGACCACA TGGGAGATTC AGTTGGCTCA CCTTTTGCTC GTGGAAGCAT GAGCGGGAAG 
GGTGATGAAA CCCCATTTAG CAAGAAAGAG CGACGTCTTC GCTTCCTTAT TCGAAAGATG 
GTAAAGACTC AGGCGTTTTA CTGGACCGTC ATTAGCCTGG TTGCACTCAA CACACTGTGT 
GTGGCTGTAG TACACTACGA TCAGCCTGAG CTACTGTCAG ACTTCCTCTA TTATGCTGAG 
TTCATTTTCC TAGGCCTCTT TATGTCAGAG ATGCTGATTA AGATGTACGG ACTGGGGACG 
CGCCCTTATT TCCACTCCTC ATTCAACTGT TTCGATTGTG CCGTCATCGT TGGCAGTATA 
TTCGAGGTGG TTTGGGCCAT GGTGAAACCC GGGACGTCCT TTGGAATCAG CGTGTTACGA 
GCTCTAAGAC TGCTTAGGAT CTTCAAAGTC ACCAAATACT GGGCATCGTT GCGCAATTTA 
GTAGTGTCTC TTCTGAACTC AATGAAGTCC ATTATCAGTT TACTGTTCCT TCTCTTCCTA 
TTCATCGTGG TCTTCGCTCT TCTTGGCATG CAGCTCTTTG GTGGACAGTT TAATTTTGAA 
CTGGGCACTC CTCCTACAAA CTTTGATACT TTCCCCGCTG CCATTATGAC AGTGTTTCAG 
ATTCTAACAG GAGAGGATTG GAATGTGGTG ATGTATGACG GCATCAAGTC TCAGGGTGGT 
GTGGACAAAG GAATGATCTT CTCCGTATTT TTCATTGTGC TCACACTTTT TGGCAACTAC 
ACACTACTGA ATGTATTCTT GGCTATCGCT GTGGACAACC TGGCCAACGC TCAGGAACTA 
ACTAAGGATG AGCAAGAGGA AGAAGAGGCC ACAACACAAA AGATGGCTCT TCAGAAAGCG 
AAAGAAGTGG CAGAAGTGAG CCCGCTGTCT GCTGCAAACC TCTCGATCGC TGCTAAAGAG 
CAGCAGAAGA ACTCGAAAGG TTCGAAGTCA GTGTGGGAGC AGCGCACAAG TGAGATCCGC 
AGACAGAACC TGATGACCAG CAGAGAGGCA CTCTATAATG AACTTGATGC TGAAGATCAC 
TGGAAGATGA ATTACCGCAA CCGGCCTGAC GTGAAGACCC ACTTGGACCG TCCACTAGTT 
GTCAATCCTC AGGAGAACCG CAACAACAAC ACCAACAAGC CCCGACCCGA TGACCCAAAC 
CCCCAGGAGC GAAGCGAAGA ACACCCGTCC AGATCCCATC ACCATCACAG CCACCATCAC 
CACCACCACC ACCACCACAC TCACCACTCC AGCCGCCAGT CTCTGCACCT CCCAGAGCTG 
GCCGAGACCG GTTTTGAATG TGATGTGGAG GGCGCAGGTG AGCACAGGCG ACCCCGACAC 
CACCAGCACA GGCGGAGGGA TGAAGATGGA GGAGAGAGAG GAAGGAAACA CAGAGATCAT 
CACAGCAGAG GAGAGACGGC TGGAGGAGAT GCAGAGGACG GACAGGAGAA GCGGCAGAGG 
AGACATCGAC ATGCTAACCA TGGGGATGGA GACGGGAGAA GGGAGAGGAG TCGACTGCAT 
CGCAATAAGA AAGAGGGTAG TTTATCGACG GCTCGTCCAA TTCAGCCAGG ACTACCTCAC 
ACTGAGAGCC AGTATAGCGA GGATCTGGAC AACCTGCGCA ACAGCAGCAA GATGGCCATT 
CACGACCCTG ACCCTGACCT CTATGACCCC TACTATGACC CAGAGCAGGC CAATAACCTG 
CTGAACCTGC GTGGACCAGA TCTACATGAC AGCAGTCTCA TTCTGACAGA CTCCAGCAAA 
CTGGAGCACA CTGCCATAGA CATGCCCCCC ATTTACCCCT CACTCAATGC CAAGCTACAA 
GTGAACAAGA ATGCCAACAC AGAGCCAGCT AAAGAAGGGG AGAAGAAAGA GGAGGAAGAA 
GACGATGGTG GCGATGAAGA AGGCCACAAG CGCATTCCAC CTTTTAGTTC TTGTTTCATC 
CTATCCACAA CCAACCCCTT CCGGAGATGC TGTCACTACA TCCTCACTCT ACGTTATTTC 
GAGATGTGTA TTCTGTCCGT TATCGCTATG AGCAGCATCG CGTTAGCAGC AGAAGATCCC 
GTCTCACAGG ATTCAGAGAG AAACAAAGTT TTGCGTTATT TTGACTACGT CTTTACTGGA 
GTGTTCACCT TTGAGATGCT TATCAAGATG GTGGTGTTAG GCTTGATTTT GCACGAGGGC 
TCATATTTTC GTGACCTGTG GAACTTTCTG GACTTTGTAG TGGTTAGTGG TGCCCTGGTA 
GCCTTTGCCT TCACGAGCTT CTCTGGGGGG AGCGGTAAGG GGAAAGACAT CAGTATCATC 
AAGTCTCTGA GGGTTCTAAG GGTGCTGCGT CCACTCAAGA CCATCAAACG TCTTCCAAAA 
CTAAAAGCGG TGTTTGACTG TGTAGTAAAC TCTCTGAAGA ATGTGTTGAA CATCCTCATT 
GTCTACATGC TGTTCATGTT CATCTTCGCT GTGGTGGCCG TGCAGCTTTT TAAAGGACGA 
TTCTTTTACT GCACTGATGA GTCTAAAGAG TTTGCCCGAG ACTGCAGGGG CGAATATTTG 
GTGTACGAGC GCGATAACGA GGTCAAATCT CAGAAGAGGG AGTGGAAGAA GTACGATTTC 
CACTACGACA ATGTGCTTTG GGCCCTGCTT ACTCTCTTTA CTGTGTCCAC CGGAGAGGGA 
TGGCCAGATG TGTTGAAGCA CTCTGTGGAC TCCACTTACG AGAATCGGGG CCCGAGCCCG 
GGATACAGGA TGGAAATGTC CATCTTTTAC GTGGTGTACT TCGTTGTCTT CCCCTTTTTC 
TTCGTAAACA TATTTGTAGC TCTCATCATC ATCACCTTCC AAGAGCAAGG AGACAAGATG 
ATGGAGGACT ACAGCCTCGA AAAAAATGAG AGAGCCTGTA TTGATTTTGC TATAAATGCC 
AAACCTCTAA CGCGCCACAT GCCTCAGAAC AAGCAAAGCT TTCAGTACCG AATGTGGCAG 
TTTGTGGTGT CGCCTCCGTT TGAGTACAGT ATCATGTCTC TCATTGCGCT CAACACCATC 
ATCCTTATGA TGAAGTATAA TGGGGCTTCC GATGCATATG ACAAAGTCCT GAAGAACCTG 
AATATAGTGT TCACCACATT CTTCTTCATG GAGAGTATTC TGAAAATCAT TGCCTTTGGT 
GTCAGAAATT TTTTCAGAGA TGCTTGGAAT ATTTTTGATT TTGTGTCTGT TATCGGCAGC 
ATCACTGATA TTTTAGTGAC TGAGCTCGTG AATAACTTCA TAAACCTGAG TTTTCTGAGA 
CTCTTTCGAG CAGCTCGTCT CATTAAGCTC TTGCGGCAGG GCGAGACCAT TCGCATTCTG 
CTTTGGACCT TCGTTCAGTC ATTTAAGGCT CTGCCATATG TATGCCTGCT CATTGCAATT 
CTGTTTTTCA TCTACGCCAT CATTGGCATG CAGCTGTTTG GGAACTTGGC TCTGGATGAC 
GAAGGTGCTA TCAATGAACA CAACAACTTC AGAACCTTCT TCATGGCCCT CATGCTCCTC 
TTCAGGAGTG CCACGGGCGA GGCGTGGCAT GATATCATGC TGTCATGTCT GGGAGGAATG 
CCCTGTGAGC CGGAGGAGGT TGGATCAGAA TGTGGCAGCA ATGTGGCCTA TGCATACTTC 
GTCTCTTTCA TCTTTCTCTG TTCGTTCCTG ATGCTGAATC TTTTTGTGGC TGTCATCATG 
GATAACTTTG AGTACTTGAC CAGGGACTCC TCCATCCTGG GTCCGCATCA TTTGGATGAG 
TATGTCAGGA TATGGGCAGA GTACGACCCC GCAGCCTGCG GGCGCATTCA TTATAAGGAT 
ATGTACAGTT TATTGCGGGT TATCGACCCC CCGCTGGGCT TAGGCAAGAA ATGTCCTCAT 
AGGGTGGCCT GCAAGAGACT GCTCCGTATG GACCTGCCCG TTGCTGATGA CAACACGGTG 
CATTTCAACT CCACTCTGAT GGCCTTGATT CGCACAGCAC TGGACATCAA AATTGCCAAG 
GGAGGAGCGG ACAAACACCA GATGGACGCG GAGCTCCGTA AAGAAATGAT GGCGATTTGG 
CCAAATCTCT CTCAGAAGAA CCTGGATCTG CTGGTAACGC CACACAAGTC AGCGACAGAC 
CTGACAGTGG GCAAGATCTA CGCCGCCATG ATGATCATGG AGTACTACCG GCAGAGCAAG 
GCCAAAAAGC TGCAGGCGCT TCGTGAGGAG CAGAACCGAA CGCCATTAAT GTTTCAGCGC 
ATGGAGCCGT CCCCAAACCA GGAAGGGGGA CCGGGGCTGA ATGGCCTGCC CGAGCTGCCG 
CAGGAGCCCG TTAACAGCCT GCCTCCAGAG GTTGGAATGA ATGAAAGCCA GTCATGGGTG 
ACGTCACGCG CCCAGGAGAT GTGCCAGAAG TCCGTTCCAG AAAACTGGAA CCCTGACAGA 
CCATATCCTG ATGACCATCA TGACAGCCGC CACAATCCAC AGACGGTAGA AATGAGAGAG 
TTTGGGCCTG TTGGGGACGG TTACTCGGAC ACTGAGCCCT ATCACCCAAT GGAAGGGCAT 
GGCCGGACCA TCTCCATGCC TCGCCTCTCT GCAGACAACC AACGACGGAG ACACAGGCCG 
CGAGGAAGTA ATCTCAGTAC CATCACCGAC ACAAGTCCAA TGCGTCGTTC TACATCCTCA 
TTGGTCCATG GACGTTCAGG TCGAGGCGTG CGATTGGATG ATTACTCTCT GGAACGGGTG 
GTGTCAGAAG AGGGTCACCG ACATGCTCCC CGAAGGAGAG ATCGTGATCG AGACCGGGAT 
AGAGATCGCA GTCATCGAAC CTCAGAGAGA TCCCTCGCTC GCTATACAGA TGCAGACACA 
GGTCTGGGTA CAGATCTTAG CACCACTACA CAGTCGGGTG ACCTCCCACC TAAAGAAAGG 
GAACGAGAAC GGGGTCGAGC AAAAGACCGT CGCCACCACC ACCACCATCA TCACCACCAC 
GGCTCGGTGG ATAAAGATCG GTACAGTCAC GAGCGCGACT ACCATCGTCA TCCTCAAGAC 
AGATCAGATC GCCAATGGTC CCGCTCTCCG AGTGAGGGCA GAGGGCACAG ACAGGGCAGT 
AGTTCTGTGA GCGGAAGCCC CGTTCCCTCG ACGTCGGGGA CCAGCACCCC CCGGCGTGGC 
CGTCGCCAGT TGCCCCAGAC CCCATCCACT CCACGCCCTC ACGTCACCTA CTCCCCTGCG 
GTCCGCAAGC CTCCATATGG CTCCCCAGGC CAGGGTCGAC TCCGCTCCCC TTCCCCGCGA 
AACTTTTCTC CGCCAGGTCA TGAACCGCCA TACCACCGTC CACCCTCCCG CCATGCCTCA 
CCTCATCCTG GCTCACCCCG GCATGCCTCT CCGCGTTCCC CCAGGCACAC GTCGCCCAGG 
TCCCCTCGTC ACGGCCGCTG GGGCCCTCCG GACAGCCTAG AAGGTGATGG AACTTTCTAC 
GAACGGGACT ATGAGTACGA GCGCCACCAT GAGCCGCCAG CCTACGAACA GAGTTTATCA 
CAGGGCAACC CCCATCCACA TTCACACCCT CATCCCCGGT CACCAAGGAC TGCTCGTCAC 
GGGCCTCAAC CACACCCGCG GCGTATGCCT AACGGCTACC GTTCCTCCTC GCCCTCCCCG 
CACCGGCGCG GCCCCCACCC AGGCCCACAC CGGCCACCGC ATGGCCGTGG GCCCCGGAAG 
GGGTTGCACG AACAATATAG CGAGACGGAC GAGGATGATT GGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335321.1
CDS	1436..8443
Translation	MARFGDEVPARYGGGGGGGSGQGGPGRGGSRQGGPPGAQRMYKQSMAQRARTMAIYNPIPVRQNCLTVNRSLFLFSEDNIVRKYAKKITEWPPFEWMILTTIIANCIVLALEQHLPVGDKTPLSERLEETEPYFIAIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSSVGSELDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGVLLFVAILMFAIIGLEFYMGKFHTTCFNTITGEMYDERPCGARTCDANNTQCQGYWLGPNYGITQFDNVLFAVLTVFQCITMEGWTDMLYYSNDALGSAWNWMYYVPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEVILAEDDNDTGSRRRPTLKKNKADLLNPQEGVDHMGDSVGSPFARGSMSGKGDETPFSKKERRLRFLIRKMVKTQAFYWTVISLVALNTLCVAVVHYDQPELLSDFLYYAEFIFLGLFMSEMLIKMYGLGTRPYFHSSFNCFDCAVIVGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFELGTPPTNFDTFPAAIMTVFQILTGEDWNVVMYDGIKSQGGVDKGMIFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEATTQKMALQKAKEVAEVSPLSAANLSIAAKEQQKNSKGSKSVWEQRTSEIRRQNLMTSREALYNELDAEDHWKMNYRNRPDVKTHLDRPLVVNPQENRNNNTNKPRPDDPNPQERSEEHPSRSHHHHSHHHHHHHHHTHHSSRQSLHLPELAETGFECDVEGAGEHRRPRHHQHRRRDEDGGERGRKHRDHHSRGETAGGDAEDGQEKRQRRHRHANHGDGDGRRERSRLHRNKKEGSLSTARPIQPGLPHTESQYSEDLDNLRNSSKMAIHDPDPDLYDPYYDPEQANNLLNLRGPDLHDSSLILTDSSKLEHTAIDMPPIYPSLNAKLQVNKNANTEPAKEGEKKEEEEDDGGDEEGHKRIPPFSSCFILSTTNPFRRCCHYILTLRYFEMCILSVIAMSSIALAAEDPVSQDSERNKVLRYFDYVFTGVFTFEMLIKMVVLGLILHEGSYFRDLWNFLDFVVVSGALVAFAFTSFSGGSGKGKDISIIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFARDCRGEYLVYERDNEVKSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPDVLKHSVDSTYENRGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYSIMSLIALNTIILMMKYNGASDAYDKVLKNLNIVFTTFFFMESILKIIAFGVRNFFRDAWNIFDFVSVIGSITDILVTELVNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAILFFIYAIIGMQLFGNLALDDEGAINEHNNFRTFFMALMLLFRSATGEAWHDIMLSCLGGMPCEPEEVGSECGSNVAYAYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKKLQALREEQNRTPLMFQRMEPSPNQEGGPGLNGLPELPQEPVNSLPPEVGMNESQSWVTSRAQEMCQKSVPENWNPDRPYPDDHHDSRHNPQTVEMREFGPVGDGYSDTEPYHPMEGHGRTISMPRLSADNQRRRHRPRGSNLSTITDTSPMRRSTSSLVHGRSGRGVRLDDYSLERVVSEEGHRHAPRRRDRDRDRDRDRSHRTSERSLARYTDADTGLGTDLSTTTQSGDLPPKERERERGRAKDRRHHHHHHHHHGSVDKDRYSHERDYHRHPQDRSDRQWSRSPSEGRGHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPSTPRPHVTYSPAVRKPPYGSPGQGRLRSPSPRNFSPPGHEPPYHRPPSRHASPHPGSPRHASPRSPRHTSPRSPRHGRWGPPDSLEGDGTFYERDYEYERHHEPPAYEQSLSQGNPHPHSHPHPRSPRTARHGPQPHPRRMPNGYRSSSPSPHRRGPHPGPHRPPHGRGPRKGLHEQYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021479646.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479646.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAGGTC ATACTCGCAG AGGATGACAA TGACACCGGA 
TCTCGACGAA GACCCACATT AAAGAAAAAT AAAGCTGACC TGTTAAATCC ACAAGAGGGA 
GTGGACCACA TGGGAGATTC AGTTGGCTCA CCTTTTGCTC GTGGAAGCAT GAGCGGGAAG 
GGTGATGAAA CCCCATTTAG CAAGAAAGAG CGACGTCTTC GCTTCCTTAT TCGAAAGATG 
GTAAAGACTC AGGCGTTTTA CTGGACCGTC ATTAGCCTGG TTGCACTCAA CACACTGTGT 
GTGGCTGTAG TACACTACGA TCAGCCTGAG CTACTGTCAG ACTTCCTCTA TTATGCTGAG 
TTCATTTTCC TAGGCCTCTT TATGTCAGAG ATGCTGATTA AGATGTACGG ACTGGGGACG 
CGCCCTTATT TCCACTCCTC ATTCAACTGT TTCGATTGTG CCGTCATCGT TGGCAGTATA 
TTCGAGGTGG TTTGGGCCAT GGTGAAACCC GGGACGTCCT TTGGAATCAG CGTGTTACGA 
GCTCTAAGAC TGCTTAGGAT CTTCAAAGTC ACCAAATACT GGGCATCGTT GCGCAATTTA 
GTAGTGTCTC TTCTGAACTC AATGAAGTCC ATTATCAGTT TACTGTTCCT TCTCTTCCTA 
TTCATCGTGG TCTTCGCTCT TCTTGGCATG CAGCTCTTTG GTGGACAGTT TAATTTTGAA 
CTGGGCACTC CTCCTACAAA CTTTGATACT TTCCCCGCTG CCATTATGAC AGTGTTTCAG 
ATTCTAACAG GAGAGGATTG GAATGTGGTG ATGTATGACG GCATCAAGTC TCAGGGTGGT 
GTGGACAAAG GAATGATCTT CTCCGTATTT TTCATTGTGC TCACACTTTT TGGCAACTAC 
ACACTACTGA ATGTATTCTT GGCTATCGCT GTGGACAACC TGGCCAACGC TCAGGAACTA 
ACTAAGGATG AGCAAGAGGA AGAAGAGGCC ACAACACAAA AGATGGCTCT TCAGAAAGCG 
AAAGAAGTGG CAGAAGTGAG CCCGCTGTCT GCTGCAAACC TCTCGATCGC TGCTAAAGAG 
CAGCAGAAGA ACTCGAAAGG TTCGAAGTCA GTGTGGGAGC AGCGCACAAG TGAGATCCGC 
AGACAGAACC TGATGACCAG CAGAGAGGCA CTCTATAATG AACTTGATGC TGAAGATCAC 
TGGAAGATGA ATTACCGCAA CCGGCCTGAC GTGAAGACCC ACTTGGACCG TCCACTAGTT 
GTCAATCCTC AGGAGAACCG CAACAACAAC ACCAACAAGC CCCGACCCGA TGACCCAAAC 
CCCCAGGAGC GAAGCGAAGA ACACCCGTCC AGATCCCATC ACCATCACAG CCACCATCAC 
CACCACCACC ACCACCACAC TCACCACTCC AGCCGCCAGT CTCTGCACCT CCCAGAGCTG 
GCCGAGACCG GTTTTGAATG TGATGTGGAG GGCGCAGGTG AGCACAGGCG ACCCCGACAC 
CACCAGCACA GGCGGAGGGA TGAAGATGGA GGAGAGAGAG GAAGGAAACA CAGAGATCAT 
CACAGCAGAG GAGAGACGGC TGGAGGAGAT GCAGAGGACG GACAGGAGAA GCGGCAGAGG 
AGACATCGAC ATGCTAACCA TGGGGATGGA GACGGGAGAA GGGAGAGGAG TCGACTGCAT 
CGCAATAAGA AAGAGGGTAG TTTATCGACG GCTCGTCCAA TTCAGCCAGG ACTACCTCAC 
ACTGAGAGCC AGTATAGCGA GGATCTGGAC AACCTGCGCA ACAGCAGCAA GATGGCCATT 
CACGACCCTG ACCCTGACCT CTATGACCCC TACTATGACC CAGAGCAGGC CAATAACCTG 
CTGAACCTGC GTGGACCAGA TCTACATGAC AGCAGTCTCA TTCTGACAGA CTCCAGCAAA 
CTGGAGCACA CTGCCATAGA CATGCCCCCC ATTTACCCCT CACTCAATGC CAAGCTACAA 
GTGAACAAGA ATGCCAACAC AGAGCCAGCT AAAGAAGGGG AGAAGAAAGA GGAGGAAGAA 
GACGATGGTG GCGATGAAGA AGGCCACAAG CGCATTCCAC CTTTTAGTTC TTGTTTCATC 
CTATCCACAA CCAACCCCTT CCGGAGATGC TGTCACTACA TCCTCACTCT ACGTTATTTC 
GAGATGTGTA TTCTGTCCGT TATCGCTATG AGCAGCATCG CGTTAGCAGC AGAAGATCCC 
GTCTCACAGG ATTCAGAGAG AAACAAAGTT TTGCGTTATT TTGACTACGT CTTTACTGGA 
GTGTTCACCT TTGAGATGCT TATCAAGATG GTGGTGTTAG GCTTGATTTT GCACGAGGGC 
TCATATTTTC GTGACCTGTG GAACTTTCTG GACTTTGTAG TGGTTAGTGG TGCCCTGGTA 
GCCTTTGCCT TCACGAGCTT CTCTGGGGGG AGCGGTAAGG GGAAAGACAT CAGTATCATC 
AAGTCTCTGA GGGTTCTAAG GGTGCTGCGT CCACTCAAGA CCATCAAACG TCTTCCAAAA 
CTAAAAGCGG TGTTTGACTG TGTAGTAAAC TCTCTGAAGA ATGTGTTGAA CATCCTCATT 
GTCTACATGC TGTTCATGTT CATCTTCGCT GTGGTGGCCG TGCAGCTTTT TAAAGGACGA 
TTCTTTTACT GCACTGATGA GTCTAAAGAG TTTGCCCGAG ACTGCAGGGG CGAATATTTG 
GTGTACGAGC GCGATAACGA GGTCAAATCT CAGAAGAGGG AGTGGAAGAA GTACGATTTC 
CACTACGACA ATGTGCTTTG GGCCCTGCTT ACTCTCTTTA CTGTGTCCAC CGGAGAGGGA 
TGGCCAGATG TGTTGAAGCA CTCTGTGGAC TCCACTTACG AGAATCGGGG CCCGAGCCCG 
GGATACAGGA TGGAAATGTC CATCTTTTAC GTGGTGTACT TCGTTGTCTT CCCCTTTTTC 
TTCGTAAACA TATTTGTAGC TCTCATCATC ATCACCTTCC AAGAGCAAGG AGACAAGATG 
ATGGAGGACT ACAGCCTCGA AAAAAATGAG AGAGCCTGTA TTGATTTTGC TATAAATGCC 
AAACCTCTAA CGCGCCACAT GCCTCAGAAC AAGCAAAGCT TTCAGTACCG AATGTGGCAG 
TTTGTGGTGT CGCCTCCGTT TGAGTACAGT ATCATGTCTC TCATTGCGCT CAACACCATC 
ATCCTTATGA TGAAGTATAA TGGGGCTTCC GATGCATATG ACAAAGTCCT GAAGAACCTG 
AATATAGTGT TCACCACATT CTTCTTCATG GAGAGTATTC TGAAAATCAT TGCCTTTGGT 
GTCAGAAATT TTTTCAGAGA TGCTTGGAAT ATTTTTGATT TTGTGTCTGT TATCGGCAGC 
ATCACTGATA TTTTAGTGAC TGAGCTCGTG AATAACTTCA TAAACCTGAG TTTTCTGAGA 
CTCTTTCGAG CAGCTCGTCT CATTAAGCTC TTGCGGCAGG GCGAGACCAT TCGCATTCTG 
CTTTGGACCT TCGTTCAGTC ATTTAAGGCT CTGCCATATG TATGCCTGCT CATTGCAATT 
CTGTTTTTCA TCTACGCCAT CATTGGCATG CAGCTGTTTG GGAACTTGGC TCTGGATGAC 
GAAGGTGCTA TCAATGAACA CAACAACTTC AGAACCTTCT TCATGGCCCT CATGCTCCTC 
TTCAGGAGTG CCACGGGCGA GGCGTGGCAT GATATCATGC TGTCATGTCT GGGAGGAATG 
CCCTGTGAGC CGGAGGAGGT TGGATCAGAA TGTGGCAGCA ATGTGGCCTA TGCATACTTC 
GTCTCTTTCA TCTTTCTCTG TTCGTTCCTG ATGCTGAATC TTTTTGTGGC TGTCATCATG 
GATAACTTTG AGTACTTGAC CAGGGACTCC TCCATCCTGG GTCCGCATCA TTTGGATGAG 
TATGTCAGGA TATGGGCAGA GTACGACCCC GCAGCCTGCG GGCGCATTCA TTATAAGGAT 
ATGTACAGTT TATTGCGGGT TATCGACCCC CCGCTGGGCT TAGGCAAGAA ATGTCCTCAT 
AGGGTGGCCT GCAAGAGACT GCTCCGTATG GACCTGCCCG TTGCTGATGA CAACACGGTG 
CATTTCAACT CCACTCTGAT GGCCTTGATT CGCACAGCAC TGGACATCAA AATTGCCAAG 
GGAGGAGCGG ACAAACACCA GATGGACGCG GAGCTCCGTA AAGAAATGAT GGCGATTTGG 
CCAAATCTCT CTCAGAAGAA CCTGGATCTG CTGGTAACGC CACACAAGTC AGCGACAGAC 
CTGACAGTGG GCAAGATCTA CGCCGCCATG ATGATCATGG AGTACTACCG GCAGAGCAAG 
GCCAAAAAGC TGCAGGCGCT TCGTGAGGAG CAGAACCGAA CGCCATTAAT GTTTCAGCGC 
ATGGAGCCGT CCCCAAACCA GGAAGGGGGA CCGGGGCTGA ATGGCCTGCC CGAGCTGCCG 
CAGGAGCCCG TTAACAGCCT GCCTCCAGAG GTTGGAATGA ATGAAAGCCA GTCATGGGTG 
ACGTCACGCG CCCAGGAGAT GTGCCAGAAG TCCGTTCCAG AAAACTGGAA CCCTGACAGA 
CCATATCCTG ATGACCATCA TGACAGCCGC CACAATCCAC AGACGGTAGA AATGAGAGAG 
TTTGGGCCTG TTGGGGACGG TTACTCGGAC ACTGAGCCCT ATCACCCAAT GGAAGGGCAT 
GGCCGGACCA TCTCCATGCC TCGCCTCTCT GCAGACAACC AACGACGGAG ACACAGGCCG 
CGAGGAAGTA ATCTCAGTAC CATCACCGAC ACAAGTCCAA TGCGTCGTTC TACATCCTCA 
TTGGTCCATG GACGTTCAGG TCGAGGCGTG CGATTGGATG ATTACTCTCT GGAACGGGTG 
GTGTCAGAAG AGGGTCACCG ACATGCTCCC CGAAGGAGAG ATCGTGATCG AGACCGGGAT 
AGAGATCGCA GTCATCGAAC CTCAGAGAGA TCCCTCGCTC GCTATACAGA TGCAGACACA 
GGTCTGGGTA CAGATCTTAG CACCACTACA CAGTCGGGTG ACCTCCCACC TAAAGAAAGG 
GAACGAGAAC GGGGTCGAGC AAAAGACCGT CGCCACCACC ACCACCATCA TCACCACCAC 
GGCTCGGTGG ATAAAGATCG GTACAGTCAC GAGCGCGACT ACCATCGTCA TCCTCAAGAC 
AGATCAGATC GCCAATGGTC CCGCTCTCCG AGTGAGGGCA GAGGGCACAG ACAGGGCAGT 
AGTTCTGTGA GCGGAAGCCC CGTTCCCTCG ACGTCGGGGA CCAGCACCCC CCGGCGTGGC 
CGTCGCCAGT TGCCCCAGAC CCCATCCACT CCACGCCCTC ACGTCACCTA CTCCCCTGCG 
GTCCGCAAGC CTCCATATGG CTCCCCAGGC CAGGGTCGAC TCCGCTCCCC TTCCCCGCGA 
AACTTTTCTC CGCCAGGTCA TGAACCGCCA TACCACCGTC CACCCTCCCG CCATGCCTCA 
CCTCATCCTG GCTCACCCCG GCATGCCTCT CCGCGTTCCC CCAGGCACAC GTCGCCCAGG 
TCCCCTCGTC ACGGCCGCTG GGGCCCTCCG GACAGCCTAG AAGGTGATGG AACTTTCTAC 
GAACGGGACT ATGAGTACGA GCGCCACCAT GAGCCGCCAG CCTACGAACA GAGTTTATCA 
CAGGGCAACC CCCATCCACA TTCACACCCT CATCCCCGGT CACCAAGGAC TGCTCGTCAC 
GGGCCTCAAC CACACCCGCG GCGTATGCCT AACGGCTACC GTTCCTCCTC GCCCTCCCCG 
CACCGGCGCG GCCCCCACCC AGGCCCACAC CGGCCACCGC ATGGCCGTGG GCCCCGGAAG 
GGGTTGCACG AACAATATAG CGAGACGGAC GAGGATGATT GGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ab ( XM_021479647.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335322.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X6, mRNA.

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CloneID	ODa147898
Clone ID Related Accession (Same CDS sequence)	XM_021479647.1
Accession Version	XM_021479647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGGG GAGCGGTAAG GGGAAAGACA TCAGTATCAT CAAGTCTCTG 
AGGGTTCTAA GGGTGCTGCG TCCACTCAAG ACCATCAAAC GTCTTCCAAA ACTAAAAGCG 
GTGTTTGACT GTGTAGTAAA CTCTCTGAAG AATGTGTTGA ACATCCTCAT TGTCTACATG 
CTGTTCATGT TCATCTTCGC TGTGGTGGCC GTGCAGCTTT TTAAAGGACG ATTCTTTTAC 
TGCACTGATG AGTCTAAAGA GTTTGCCCGA GACTGCAGGG GCGAATATTT GGTGTACGAG 
CGCGATAACG AGGTCAAATC TCAGAAGAGG GAGTGGAAGA AGTACGATTT CCACTACGAC 
AATGTGCTTT GGGCCCTGCT TACTCTCTTT ACTGTGTCCA CCGGAGAGGG ATGGCCAGAT 
GTGTTGAAGC ACTCTGTGGA CTCCACTTAC GAGAATCGGG GCCCGAGCCC GGGATACAGG 
ATGGAAATGT CCATCTTTTA CGTGGTGTAC TTCGTTGTCT TCCCCTTTTT CTTCGTAAAC 
ATATTTGTAG CTCTCATCAT CATCACCTTC CAAGAGCAAG GAGACAAGAT GATGGAGGAC 
TACAGCCTCG AAAAAAATGA GAGAGCCTGT ATTGATTTTG CTATAAATGC CAAACCTCTA 
ACGCGCCACA TGCCTCAGAA CAAGCAAAGC TTTCAGTACC GAATGTGGCA GTTTGTGGTG 
TCGCCTCCGT TTGAGTACAG TATCATGTCT CTCATTGCGC TCAACACCAT CATCCTTATG 
ATGAAGTATA ATGGGGCTTC CGATGCATAT GACAAAGTCC TGAAGAACCT GAATATAGTG 
TTCACCACAT TCTTCTTCAT GGAGAGTATT CTGAAAATCA TTGCCTTTGG TGTCAGAAAT 
TTTTTCAGAG ATGCTTGGAA TATTTTTGAT TTTGTGTCTG TTATCGGCAG CATCACTGAT 
ATTTTAGTGA CTGAGCTCGT GAATAACTTC ATAAACCTGA GTTTTCTGAG ACTCTTTCGA 
GCAGCTCGTC TCATTAAGCT CTTGCGGCAG GGCGAGACCA TTCGCATTCT GCTTTGGACC 
TTCGTTCAGT CATTTAAGGC TCTGCCATAT GTATGCCTGC TCATTGCAAT TCTGTTTTTC 
ATCTACGCCA TCATTGGCAT GCAGCTGTTT GGGAACTTGG CTCTGGATGA CGAAGGTGCT 
ATCAATGAAC ACAACAACTT CAGAACCTTC TTCATGGCCC TCATGCTCCT CTTCAGGAGT 
GCCACGGGCG AGGCGTGGCA TGATATCATG CTGTCATGTC TGGGAGGAAT GCCCTGTGAG 
CCGGAGGAGG TTGGATCAGA ATGTGGCAGC AATGTGGCCT ATGCATACTT CGTCTCTTTC 
ATCTTTCTCT GTTCGTTCCT GATGCTGAAT CTTTTTGTGG CTGTCATCAT GGATAACTTT 
GAGTACTTGA CCAGGGACTC CTCCATCCTG GGTCCGCATC ATTTGGATGA GTATGTCAGG 
ATATGGGCAG AGTACGACCC CGCAGCCTGC GGGCGCATTC ATTATAAGGA TATGTACAGT 
TTATTGCGGG TTATCGACCC CCCGCTGGGC TTAGGCAAGA AATGTCCTCA TAGGGTGGCC 
TGCAAGAGAC TGCTCCGTAT GGACCTGCCC GTTGCTGATG ACAACACGGT GCATTTCAAC 
TCCACTCTGA TGGCCTTGAT TCGCACAGCA CTGGACATCA AAATTGCCAA GGGAGGAGCG 
GACAAACACC AGATGGACGC GGAGCTCCGT AAAGAAATGA TGGCGATTTG GCCAAATCTC 
TCTCAGAAGA ACCTGGATCT GCTGGTAACG CCACACAAGT CAGCGACAGA CCTGACAGTG 
GGCAAGATCT ACGCCGCCAT GATGATCATG GAGTACTACC GGCAGAGCAA GGCCAAAAAG 
CTGCAGGCGC TTCGTGAGGA GCAGAACCGA ACGCCATTAA TGTTTCAGCG CATGGAGCCG 
TCCCCAAACC AGGAAGGGGG ACCGGGGCTG AATGGCCTGC CCGAGCTGCC GCAGGAGCCC 
GTTAACAGCC TGCCTCCAGA GGTTGGAATG AATGAAAGCC AGTCATGGGT GACGTCACGC 
GCCCAGGAGA TGTGCCAGAA GTCCGTTCCA GAAAACTGGA ACCCTGACAG ACCATATCCT 
GATGACCATC ATGACAGCCG CCACAATCCA CAGACGGTAG AAATGAGAGA GTTTGGGCCT 
GTTGGGGACG GTTACTCGGA CACTGAGCCC TATCACCCAA TGGAAGGGCA TGGCCGGACC 
ATCTCCATGC CTCGCCTCTC TGCAGACAAC CAACGACGGA GACACAGGCC GCGAGGAAGT 
AATCTCAGTA CCATCACCGA CACAAGTCCA ATGCGTCGTT CTACATCCTC ATTGGTCCAT 
GGACGTTCAG GTCGAGGCGT GCGATTGGAT GATTACTCTC TGGAACGGGT GGTGTCAGAA 
GAGGGTCACC GACATGCTCC CCGAAGGAGA GATCGTGATC GAGACCGGGA TAGAGATCGC 
AGTCATCGAA CCTCAGAGAG ATCCCTCGCT CGCTATACAG ATGCAGACAC AGGTCTGGGT 
ACAGATCTTA GCACCACTAC ACAGTCGGGT GACCTCCCAC CTAAAGAAAG GGAACGAGAA 
CGGGGTCGAG CAAAAGACCG TCGCCACCAC CACCACCATC ATCACCACCA CGGCTCGGTG 
GATAAAGATC GGTACAGTCA CGAGCGCGAC TACCATCGTC ATCCTCAAGA CAGATCAGAT 
CGCCAATGGT CCCGCTCTCC GAGTGAGGGC AGAGGGCACA GACAGGGCAG TAGTTCTGTG 
AGCGGAAGCC CCGTTCCCTC GACGTCGGGG ACCAGCACCC CCCGGCGTGG CCGTCGCCAG 
TTGCCCCAGA CCCCATCCAC TCCACGCCCT CACGTCACCT ACTCCCCTGC GGTCCGCAAG 
CCTCCATATG GCTCCCCAGG CCAGGGTCGA CTCCGCTCCC CTTCCCCGCG AAACTTTTCT 
CCGCCAGGTC ATGAACCGCC ATACCACCGT CCACCCTCCC GCCATGCCTC ACCTCATCCT 
GGCTCACCCC GGCATGCCTC TCCGCGTTCC CCCAGGCACA CGTCGCCCAG GTCCCCTCGT 
CACGGCCGCT GGGGCCCTCC GGACAGCCTA GAAGGTGATG GAACTTTCTA CGAACGGGAC 
TATGAGTACG AGCGCCACCA TGAGCCGCCA GCCTACGAAC AGAGTTTATC ACAGGGCAAC 
CCCCATCCAC ATTCACACCC TCATCCCCGG TCACCAAGGA CTGCTCGTCA CGGGCCTCAA 
CCACACCCGC GGCGTATGCC TAACGGCTAC CGTTCCTCCT CGCCCTCCCC GCACCGGCGC 
GGCCCCCACC CAGGCCCACA CCGGCCACCG CATGGCCGTG GGCCCCGGAA GGGGTTGCAC 
GAACAATATA GCGAGACGGA CGAGGATGAT TGGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335322.1
CDS	1436..8434
Translation	MARFGDEVPARYGGGGGGGSGQGGPGRGGSRQGGPPGAQRMYKQSMAQRARTMAIYNPIPVRQNCLTVNRSLFLFSEDNIVRKYAKKITEWPPFEWMILTTIIANCIVLALEQHLPVGDKTPLSERLEETEPYFIAIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSSVGSELDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGVLLFVAILMFAIIGLEFYMGKFHTTCFNTITGEMYDERPCGARTCDANNTQCQGYWLGPNYGITQFDNVLFAVLTVFQCITMEGWTDMLYYSNDALGSAWNWMYYVPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILAEDDNDTGSRRRPTLKKNKADLLNPQEGVDHMGDSVGSPFARGSMSGKGDETPFSKKERRLRFLIRKMVKTQAFYWTVISLVALNTLCVAVVHYDQPELLSDFLYYAEFIFLGLFMSEMLIKMYGLGTRPYFHSSFNCFDCAVIVGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFELGTPPTNFDTFPAAIMTVFQILTGEDWNVVMYDGIKSQGGVDKGMIFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEATTQKMALQKAKEVAEVSPLSAANLSIAAKEQQKNSKGSKSVWEQRTSEIRRQNLMTSREALYNELDAEDHWKMNYRNRPDVKTHLDRPLVVNPQENRNNNTNKPRPDDPNPQERSEEHPSRSHHHHSHHHHHHHHHTHHSSRQSLHLPELAETGFECDVEGAGEHRRPRHHQHRRRDEDGGERGRKHRDHHSRGETAGGDAEDGQEKRQRRHRHANHGDGDGRRERSRLHRNKKEGSLSTARPIQPGLPHTESQYSEDLDNLRNSSKMAIHDPDPDLYDPYYDPEQANNLLNLRGPDLHDSSLILTDSSKLEHTAIDMPPIYPSLNAKLQVNKNANTEPAKEGEKKEEEEDDGGDEEGHKRIPPFSSCFILSTTNPFRRCCHYILTLRYFEMCILSVIAMSSIALAAEDPVSQDSERNKVLRYFDYVFTGVFTFEMLIKMVVLGLILHEGSYFRDLWNFLDFVVVSGALVAFAFTGSGKGKDISIIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFARDCRGEYLVYERDNEVKSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPDVLKHSVDSTYENRGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYSIMSLIALNTIILMMKYNGASDAYDKVLKNLNIVFTTFFFMESILKIIAFGVRNFFRDAWNIFDFVSVIGSITDILVTELVNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAILFFIYAIIGMQLFGNLALDDEGAINEHNNFRTFFMALMLLFRSATGEAWHDIMLSCLGGMPCEPEEVGSECGSNVAYAYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKKLQALREEQNRTPLMFQRMEPSPNQEGGPGLNGLPELPQEPVNSLPPEVGMNESQSWVTSRAQEMCQKSVPENWNPDRPYPDDHHDSRHNPQTVEMREFGPVGDGYSDTEPYHPMEGHGRTISMPRLSADNQRRRHRPRGSNLSTITDTSPMRRSTSSLVHGRSGRGVRLDDYSLERVVSEEGHRHAPRRRDRDRDRDRDRSHRTSERSLARYTDADTGLGTDLSTTTQSGDLPPKERERERGRAKDRRHHHHHHHHHGSVDKDRYSHERDYHRHPQDRSDRQWSRSPSEGRGHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPSTPRPHVTYSPAVRKPPYGSPGQGRLRSPSPRNFSPPGHEPPYHRPPSRHASPHPGSPRHASPRSPRHTSPRSPRHGRWGPPDSLEGDGTFYERDYEYERHHEPPAYEQSLSQGNPHPHSHPHPRSPRTARHGPQPHPRRMPNGYRSSSPSPHRRGPHPGPHRPPHGRGPRKGLHEQYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021479647.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479647.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGGG GAGCGGTAAG GGGAAAGACA TCAGTATCAT CAAGTCTCTG 
AGGGTTCTAA GGGTGCTGCG TCCACTCAAG ACCATCAAAC GTCTTCCAAA ACTAAAAGCG 
GTGTTTGACT GTGTAGTAAA CTCTCTGAAG AATGTGTTGA ACATCCTCAT TGTCTACATG 
CTGTTCATGT TCATCTTCGC TGTGGTGGCC GTGCAGCTTT TTAAAGGACG ATTCTTTTAC 
TGCACTGATG AGTCTAAAGA GTTTGCCCGA GACTGCAGGG GCGAATATTT GGTGTACGAG 
CGCGATAACG AGGTCAAATC TCAGAAGAGG GAGTGGAAGA AGTACGATTT CCACTACGAC 
AATGTGCTTT GGGCCCTGCT TACTCTCTTT ACTGTGTCCA CCGGAGAGGG ATGGCCAGAT 
GTGTTGAAGC ACTCTGTGGA CTCCACTTAC GAGAATCGGG GCCCGAGCCC GGGATACAGG 
ATGGAAATGT CCATCTTTTA CGTGGTGTAC TTCGTTGTCT TCCCCTTTTT CTTCGTAAAC 
ATATTTGTAG CTCTCATCAT CATCACCTTC CAAGAGCAAG GAGACAAGAT GATGGAGGAC 
TACAGCCTCG AAAAAAATGA GAGAGCCTGT ATTGATTTTG CTATAAATGC CAAACCTCTA 
ACGCGCCACA TGCCTCAGAA CAAGCAAAGC TTTCAGTACC GAATGTGGCA GTTTGTGGTG 
TCGCCTCCGT TTGAGTACAG TATCATGTCT CTCATTGCGC TCAACACCAT CATCCTTATG 
ATGAAGTATA ATGGGGCTTC CGATGCATAT GACAAAGTCC TGAAGAACCT GAATATAGTG 
TTCACCACAT TCTTCTTCAT GGAGAGTATT CTGAAAATCA TTGCCTTTGG TGTCAGAAAT 
TTTTTCAGAG ATGCTTGGAA TATTTTTGAT TTTGTGTCTG TTATCGGCAG CATCACTGAT 
ATTTTAGTGA CTGAGCTCGT GAATAACTTC ATAAACCTGA GTTTTCTGAG ACTCTTTCGA 
GCAGCTCGTC TCATTAAGCT CTTGCGGCAG GGCGAGACCA TTCGCATTCT GCTTTGGACC 
TTCGTTCAGT CATTTAAGGC TCTGCCATAT GTATGCCTGC TCATTGCAAT TCTGTTTTTC 
ATCTACGCCA TCATTGGCAT GCAGCTGTTT GGGAACTTGG CTCTGGATGA CGAAGGTGCT 
ATCAATGAAC ACAACAACTT CAGAACCTTC TTCATGGCCC TCATGCTCCT CTTCAGGAGT 
GCCACGGGCG AGGCGTGGCA TGATATCATG CTGTCATGTC TGGGAGGAAT GCCCTGTGAG 
CCGGAGGAGG TTGGATCAGA ATGTGGCAGC AATGTGGCCT ATGCATACTT CGTCTCTTTC 
ATCTTTCTCT GTTCGTTCCT GATGCTGAAT CTTTTTGTGG CTGTCATCAT GGATAACTTT 
GAGTACTTGA CCAGGGACTC CTCCATCCTG GGTCCGCATC ATTTGGATGA GTATGTCAGG 
ATATGGGCAG AGTACGACCC CGCAGCCTGC GGGCGCATTC ATTATAAGGA TATGTACAGT 
TTATTGCGGG TTATCGACCC CCCGCTGGGC TTAGGCAAGA AATGTCCTCA TAGGGTGGCC 
TGCAAGAGAC TGCTCCGTAT GGACCTGCCC GTTGCTGATG ACAACACGGT GCATTTCAAC 
TCCACTCTGA TGGCCTTGAT TCGCACAGCA CTGGACATCA AAATTGCCAA GGGAGGAGCG 
GACAAACACC AGATGGACGC GGAGCTCCGT AAAGAAATGA TGGCGATTTG GCCAAATCTC 
TCTCAGAAGA ACCTGGATCT GCTGGTAACG CCACACAAGT CAGCGACAGA CCTGACAGTG 
GGCAAGATCT ACGCCGCCAT GATGATCATG GAGTACTACC GGCAGAGCAA GGCCAAAAAG 
CTGCAGGCGC TTCGTGAGGA GCAGAACCGA ACGCCATTAA TGTTTCAGCG CATGGAGCCG 
TCCCCAAACC AGGAAGGGGG ACCGGGGCTG AATGGCCTGC CCGAGCTGCC GCAGGAGCCC 
GTTAACAGCC TGCCTCCAGA GGTTGGAATG AATGAAAGCC AGTCATGGGT GACGTCACGC 
GCCCAGGAGA TGTGCCAGAA GTCCGTTCCA GAAAACTGGA ACCCTGACAG ACCATATCCT 
GATGACCATC ATGACAGCCG CCACAATCCA CAGACGGTAG AAATGAGAGA GTTTGGGCCT 
GTTGGGGACG GTTACTCGGA CACTGAGCCC TATCACCCAA TGGAAGGGCA TGGCCGGACC 
ATCTCCATGC CTCGCCTCTC TGCAGACAAC CAACGACGGA GACACAGGCC GCGAGGAAGT 
AATCTCAGTA CCATCACCGA CACAAGTCCA ATGCGTCGTT CTACATCCTC ATTGGTCCAT 
GGACGTTCAG GTCGAGGCGT GCGATTGGAT GATTACTCTC TGGAACGGGT GGTGTCAGAA 
GAGGGTCACC GACATGCTCC CCGAAGGAGA GATCGTGATC GAGACCGGGA TAGAGATCGC 
AGTCATCGAA CCTCAGAGAG ATCCCTCGCT CGCTATACAG ATGCAGACAC AGGTCTGGGT 
ACAGATCTTA GCACCACTAC ACAGTCGGGT GACCTCCCAC CTAAAGAAAG GGAACGAGAA 
CGGGGTCGAG CAAAAGACCG TCGCCACCAC CACCACCATC ATCACCACCA CGGCTCGGTG 
GATAAAGATC GGTACAGTCA CGAGCGCGAC TACCATCGTC ATCCTCAAGA CAGATCAGAT 
CGCCAATGGT CCCGCTCTCC GAGTGAGGGC AGAGGGCACA GACAGGGCAG TAGTTCTGTG 
AGCGGAAGCC CCGTTCCCTC GACGTCGGGG ACCAGCACCC CCCGGCGTGG CCGTCGCCAG 
TTGCCCCAGA CCCCATCCAC TCCACGCCCT CACGTCACCT ACTCCCCTGC GGTCCGCAAG 
CCTCCATATG GCTCCCCAGG CCAGGGTCGA CTCCGCTCCC CTTCCCCGCG AAACTTTTCT 
CCGCCAGGTC ATGAACCGCC ATACCACCGT CCACCCTCCC GCCATGCCTC ACCTCATCCT 
GGCTCACCCC GGCATGCCTC TCCGCGTTCC CCCAGGCACA CGTCGCCCAG GTCCCCTCGT 
CACGGCCGCT GGGGCCCTCC GGACAGCCTA GAAGGTGATG GAACTTTCTA CGAACGGGAC 
TATGAGTACG AGCGCCACCA TGAGCCGCCA GCCTACGAAC AGAGTTTATC ACAGGGCAAC 
CCCCATCCAC ATTCACACCC TCATCCCCGG TCACCAAGGA CTGCTCGTCA CGGGCCTCAA 
CCACACCCGC GGCGTATGCC TAACGGCTAC CGTTCCTCCT CGCCCTCCCC GCACCGGCGC 
GGCCCCCACC CAGGCCCACA CCGGCCACCG CATGGCCGTG GGCCCCGGAA GGGGTTGCAC 
GAACAATATA GCGAGACGGA CGAGGATGAT TGGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ab ( XM_021479648.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335323.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X7, mRNA.

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CloneID	ODa147899
Clone ID Related Accession (Same CDS sequence)	XM_021479648.1
Accession Version	XM_021479648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGGCGCAT TCATTATAAG 
GATATGTACA GTTTATTGCG GGTTATCGAC CCCCCGCTGG GCTTAGGCAA GAAATGTCCT 
CATAGGGTGG CCTGCAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCAGCGACA 
GACCTGACAG TGGGCAAGAT CTACGCCGCC ATGATGATCA TGGAGTACTA CCGGCAGAGC 
AAGGCCAAAA AGCTGCAGGC GCTTCGTGAG GAGCAGAACC GAACGCCATT AATGTTTCAG 
CGCATGGAGC CGTCCCCAAA CCAGGAAGGG GGACCGGGGC TGAATGGCCT GCCCGAGCTG 
CCGCAGGAGC CCGTTAACAG CCTGCCTCCA GAGGTTGGAA TGAATGAAAG CCAGTCATGG 
GTGACGTCAC GCGCCCAGGA GATGTGCCAG AAGTCCGTTC CAGAAAACTG GAACCCTGAC 
AGACCATATC CTGATGACCA TCATGACAGC CGCCACAATC CACAGACGGT AGAAATGAGA 
GAGTTTGGGC CTGTTGGGGA CGGTTACTCG GACACTGAGC CCTATCACCC AATGGAAGGG 
CATGGCCGGA CCATCTCCAT GCCTCGCCTC TCTGCAGACA ACCAACGACG GAGACACAGG 
CCGCGAGGAA GTAATCTCAG TACCATCACC GACACAAGTC CAATGCGTCG TTCTACATCC 
TCATTGGTCC ATGGACGTTC AGGTCGAGGC GTGCGATTGG ATGATTACTC TCTGGAACGG 
GTGGTGTCAG AAGAGGGTCA CCGACATGCT CCCCGAAGGA GAGATCGTGA TCGAGACCGG 
GATAGAGATC GCAGTCATCG AACCTCAGAG AGATCCCTCG CTCGCTATAC AGATGCAGAC 
ACAGATCTTA GCACCACTAC ACAGTCGGGT GACCTCCCAC CTAAAGAAAG GGAACGAGAA 
CGGGGTCGAG CAAAAGACCG TCGCCACCAC CACCACCATC ATCACCACCA CGGCTCGGTG 
GATAAAGATC GGTACAGTCA CGAGCGCGAC TACCATCGTC ATCCTCAAGA CAGATCAGAT 
CGCCAATGGT CCCGCTCTCC GAGTGAGGGC AGAGGGCACA GACAGGGCAG TAGTTCTGTG 
AGCGGAAGCC CCGTTCCCTC GACGTCGGGG ACCAGCACCC CCCGGCGTGG CCGTCGCCAG 
TTGCCCCAGA CCCCATCCAC TCCACGCCCT CACGTCACCT ACTCCCCTGC GGTCCGCAAG 
CCTCCATATG GCTCCCCAGG CCAGGGTCGA CTCCGCTCCC CTTCCCCGCG AAACTTTTCT 
CCGCCAGGTC ATGAACCGCC ATACCACCGT CCACCCTCCC GCCATGCCTC ACCTCATCCT 
GGCTCACCCC GGCATGCCTC TCCGCGTTCC CCCAGGCACA CGTCGCCCAG GTCCCCTCGT 
CACGGCCGCT GGGGCCCTCC GGACAGCCTA GAAGGTGATG GAACTTTCTA CGAACGGGAC 
TATGAGTACG AGCGCCACCA TGAGCCGCCA GCCTACGAAC AGAGTTTATC ACAGGGCAAC 
CCCCATCCAC ATTCACACCC TCATCCCCGG TCACCAAGGA CTGCTCGTCA CGGGCCTCAA 
CCACACCCGC GGCGTATGCC TAACGGCTAC CGTTCCTCCT CGCCCTCCCC GCACCGGCGC 
GGCCCCCACC CAGGCCCACA CCGGCCACCG CATGGCCGTG GGCCCCGGAA GGGGTTGCAC 
GAACAATATA GCGAGACGGA CGAGGATGAT TGGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335323.1
CDS	1438..8436
Translation	MARFGDEVPARYGGGGGGGSGQGGPGRGGSRQGGPPGAQRMYKQSMAQRARTMAIYNPIPVRQNCLTVNRSLFLFSEDNIVRKYAKKITEWPPFEWMILTTIIANCIVLALEQHLPVGDKTPLSERLEETEPYFIAIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSSVGSELDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGVLLFVAILMFAIIGLEFYMGKFHTTCFNTITGEMYDERPCGARTCDANNTQCQGYWLGPNYGITQFDNVLFAVLTVFQCITMEGWTDMLYYSNDALGSAWNWMYYVPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILAEDDNDTGSRRRPTLKKNKADLLNPQEGVDHMGDSVGSPFARGSMSGKGDETPFSKKERRLRFLIRKMVKTQAFYWTVISLVALNTLCVAVVHYDQPELLSDFLYYAEFIFLGLFMSEMLIKMYGLGTRPYFHSSFNCFDCAVIVGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFELGTPPTNFDTFPAAIMTVFQILTGEDWNVVMYDGIKSQGGVDKGMIFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEATTQKMALQKAKEVAEVSPLSAANLSIAAKEQQKNSKGSKSVWEQRTSEIRRQNLMTSREALYNELDAEDHWKMNYRNRPDVKTHLDRPLVVNPQENRNNNTNKPRPDDPNPQERSEEHPSRSHHHHSHHHHHHHHHTHHSSRQSLHLPELAETGFECDVEGAGEHRRPRHHQHRRRDEDGGERGRKHRDHHSRGETAGGDAEDGQEKRQRRHRHANHGDGDGRRERSRLHRNKKEGSLSTARPIQPGLPHTESQYSEDLDNLRNSSKMAIHDPDPDLYDPYYDPEQANNLLNLRGPDLHDSSLILTDSSKLEHTAIDMPPIYPSLNAKLQVNKNANTEPAKEGEKKEEEEDDGGDEEGHKRIPPFSSCFILSTTNPFRRCCHYILTLRYFEMCILSVIAMSSIALAAEDPVSQDSERNKVLRYFDYVFTGVFTFEMLIKMVVLGLILHEGSYFRDLWNFLDFVVVSGALVAFAFTSFSGGSGKGKDISIIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFARDCRGEYLVYERDNEVKSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPDVLKHSVDSTYENRGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYSIMSLIALNTIILMMKYNGASDAYDKVLKNLNIVFTTFFFMESILKIIAFGVRNFFRDAWNIFDFVSVIGSITDILVTELVNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAILFFIYAIIGMQLFGNLALDDEGAINEHNNFRTFFMALMLLFRSATGEAWHDIMLSCLGGMPCEPEEVGSECGSNVAYAYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKKLQALREEQNRTPLMFQRMEPSPNQEGGPGLNGLPELPQEPVNSLPPEVGMNESQSWVTSRAQEMCQKSVPENWNPDRPYPDDHHDSRHNPQTVEMREFGPVGDGYSDTEPYHPMEGHGRTISMPRLSADNQRRRHRPRGSNLSTITDTSPMRRSTSSLVHGRSGRGVRLDDYSLERVVSEEGHRHAPRRRDRDRDRDRDRSHRTSERSLARYTDADTDLSTTTQSGDLPPKERERERGRAKDRRHHHHHHHHHGSVDKDRYSHERDYHRHPQDRSDRQWSRSPSEGRGHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPSTPRPHVTYSPAVRKPPYGSPGQGRLRSPSPRNFSPPGHEPPYHRPPSRHASPHPGSPRHASPRSPRHTSPRSPRHGRWGPPDSLEGDGTFYERDYEYERHHEPPAYEQSLSQGNPHPHSHPHPRSPRTARHGPQPHPRRMPNGYRSSSPSPHRRGPHPGPHRPPHGRGPRKGLHEQYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021479648.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479648.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGGCGCAT TCATTATAAG 
GATATGTACA GTTTATTGCG GGTTATCGAC CCCCCGCTGG GCTTAGGCAA GAAATGTCCT 
CATAGGGTGG CCTGCAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCAGCGACA 
GACCTGACAG TGGGCAAGAT CTACGCCGCC ATGATGATCA TGGAGTACTA CCGGCAGAGC 
AAGGCCAAAA AGCTGCAGGC GCTTCGTGAG GAGCAGAACC GAACGCCATT AATGTTTCAG 
CGCATGGAGC CGTCCCCAAA CCAGGAAGGG GGACCGGGGC TGAATGGCCT GCCCGAGCTG 
CCGCAGGAGC CCGTTAACAG CCTGCCTCCA GAGGTTGGAA TGAATGAAAG CCAGTCATGG 
GTGACGTCAC GCGCCCAGGA GATGTGCCAG AAGTCCGTTC CAGAAAACTG GAACCCTGAC 
AGACCATATC CTGATGACCA TCATGACAGC CGCCACAATC CACAGACGGT AGAAATGAGA 
GAGTTTGGGC CTGTTGGGGA CGGTTACTCG GACACTGAGC CCTATCACCC AATGGAAGGG 
CATGGCCGGA CCATCTCCAT GCCTCGCCTC TCTGCAGACA ACCAACGACG GAGACACAGG 
CCGCGAGGAA GTAATCTCAG TACCATCACC GACACAAGTC CAATGCGTCG TTCTACATCC 
TCATTGGTCC ATGGACGTTC AGGTCGAGGC GTGCGATTGG ATGATTACTC TCTGGAACGG 
GTGGTGTCAG AAGAGGGTCA CCGACATGCT CCCCGAAGGA GAGATCGTGA TCGAGACCGG 
GATAGAGATC GCAGTCATCG AACCTCAGAG AGATCCCTCG CTCGCTATAC AGATGCAGAC 
ACAGATCTTA GCACCACTAC ACAGTCGGGT GACCTCCCAC CTAAAGAAAG GGAACGAGAA 
CGGGGTCGAG CAAAAGACCG TCGCCACCAC CACCACCATC ATCACCACCA CGGCTCGGTG 
GATAAAGATC GGTACAGTCA CGAGCGCGAC TACCATCGTC ATCCTCAAGA CAGATCAGAT 
CGCCAATGGT CCCGCTCTCC GAGTGAGGGC AGAGGGCACA GACAGGGCAG TAGTTCTGTG 
AGCGGAAGCC CCGTTCCCTC GACGTCGGGG ACCAGCACCC CCCGGCGTGG CCGTCGCCAG 
TTGCCCCAGA CCCCATCCAC TCCACGCCCT CACGTCACCT ACTCCCCTGC GGTCCGCAAG 
CCTCCATATG GCTCCCCAGG CCAGGGTCGA CTCCGCTCCC CTTCCCCGCG AAACTTTTCT 
CCGCCAGGTC ATGAACCGCC ATACCACCGT CCACCCTCCC GCCATGCCTC ACCTCATCCT 
GGCTCACCCC GGCATGCCTC TCCGCGTTCC CCCAGGCACA CGTCGCCCAG GTCCCCTCGT 
CACGGCCGCT GGGGCCCTCC GGACAGCCTA GAAGGTGATG GAACTTTCTA CGAACGGGAC 
TATGAGTACG AGCGCCACCA TGAGCCGCCA GCCTACGAAC AGAGTTTATC ACAGGGCAAC 
CCCCATCCAC ATTCACACCC TCATCCCCGG TCACCAAGGA CTGCTCGTCA CGGGCCTCAA 
CCACACCCGC GGCGTATGCC TAACGGCTAC CGTTCCTCCT CGCCCTCCCC GCACCGGCGC 
GGCCCCCACC CAGGCCCACA CCGGCCACCG CATGGCCGTG GGCCCCGGAA GGGGTTGCAC 
GAACAATATA GCGAGACGGA CGAGGATGAT TGGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ab ( XM_017358346.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213835.2 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X8, mRNA.

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CloneID	ODa147900
Clone ID Related Accession (Same CDS sequence)	XM_017358346.2
Accession Version	XM_017358346.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358346.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGGG GAGCGGTAAG GGGAAAGACA TCAGTATCAT CAAGTCTCTG 
AGGGTTCTAA GGGTGCTGCG TCCACTCAAG ACCATCAAAC GTCTTCCAAA ACTAAAAGCG 
GTGTTTGACT GTGTAGTAAA CTCTCTGAAG AATGTGTTGA ACATCCTCAT TGTCTACATG 
CTGTTCATGT TCATCTTCGC TGTGGTGGCC GTGCAGCTTT TTAAAGGACG ATTCTTTTAC 
TGCACTGATG AGTCTAAAGA GTTTGCCCGA GACTGCAGGG GCGAATATTT GGTGTACGAG 
CGCGATAACG AGGTCAAATC TCAGAAGAGG GAGTGGAAGA AGTACGATTT CCACTACGAC 
AATGTGCTTT GGGCCCTGCT TACTCTCTTT ACTGTGTCCA CCGGAGAGGG ATGGCCAGAT 
GTGTTGAAGC ACTCTGTGGA CTCCACTTAC GAGAATCGGG GCCCGAGCCC GGGATACAGG 
ATGGAAATGT CCATCTTTTA CGTGGTGTAC TTCGTTGTCT TCCCCTTTTT CTTCGTAAAC 
ATATTTGTAG CTCTCATCAT CATCACCTTC CAAGAGCAAG GAGACAAGAT GATGGAGGAC 
TACAGCCTCG AAAAAAATGA GAGAGCCTGT ATTGATTTTG CTATAAATGC CAAACCTCTA 
ACGCGCCACA TGCCTCAGAA CAAGCAAAGC TTTCAGTACC GAATGTGGCA GTTTGTGGTG 
TCGCCTCCGT TTGAGTACAG TATCATGTCT CTCATTGCGC TCAACACCAT CATCCTTATG 
ATGAAGTATA ATGGGGCTTC CGATGCATAT GACAAAGTCC TGAAGAACCT GAATATAGTG 
TTCACCACAT TCTTCTTCAT GGAGAGTATT CTGAAAATCA TTGCCTTTGG TGTCAGAAAT 
TTTTTCAGAG ATGCTTGGAA TATTTTTGAT TTTGTGTCTG TTATCGGCAG CATCACTGAT 
ATTTTAGTGA CTGAGCTCGT GAATAACTTC ATAAACCTGA GTTTTCTGAG ACTCTTTCGA 
GCAGCTCGTC TCATTAAGCT CTTGCGGCAG GGCGAGACCA TTCGCATTCT GCTTTGGACC 
TTCGTTCAGT CATTTAAGGC TCTGCCATAT GTATGCCTGC TCATTGCAAT TCTGTTTTTC 
ATCTACGCCA TCATTGGCAT GCAGCTGTTT GGGAACTTGG CTCTGGATGA CGAAGGTGCT 
ATCAATGAAC ACAACAACTT CAGAACCTTC TTCATGGCCC TCATGCTCCT CTTCAGGAGT 
GCCACGGGCG AGGCGTGGCA TGATATCATG CTGTCATGTC TGGGAGGAAT GCCCTGTGAG 
CCGGAGGAGG TTGGATCAGA ATGTGGCAGC AATGTGGCCT ATGCATACTT CGTCTCTTTC 
ATCTTTCTCT GTTCGTTCCT GATGCTGAAT CTTTTTGTGG CTGTCATCAT GGATAACTTT 
GAGTACTTGA CCAGGGACTC CTCCATCCTG GGTCCGCATC ATTTGGATGA GTATGTCAGG 
ATATGGGCAG AGTACGACCC CGCAGCCTGC GGGCGCATTC ATTATAAGGA TATGTACAGT 
TTATTGCGGG TTATCGACCC CCCGCTGGGC TTAGGCAAGA AATGTCCTCA TAGGGTGGCC 
TGCAAGAGAC TGCTCCGTAT GGACCTGCCC GTTGCTGATG ACAACACGGT GCATTTCAAC 
TCCACTCTGA TGGCCTTGAT TCGCACAGCA CTGGACATCA AAATTGCCAA GGGAGGAGCG 
GACAAACACC AGATGGACGC GGAGCTCCGT AAAGAAATGA TGGCGATTTG GCCAAATCTC 
TCTCAGAAGA ACCTGGATCT GCTGGTAACG CCACACAAGT CGACAGACCT GACAGTGGGC 
AAGATCTACG CCGCCATGAT GATCATGGAG TACTACCGGC AGAGCAAGGC CAAAAAGCTG 
CAGGCGCTTC GTGAGGAGCA GAACCGAACG CCATTAATGT TTCAGCGCAT GGAGCCGTCC 
CCAAACCAGG AAGGGGGACC GGGGCTGAAT GGCCTGCCCG AGCTGCCGCA GGAGCCCGTT 
AACAGCCTGC CTCCAGAGGT TGGAATGAAT GAAAGCCAGT CATGGGTGAC GTCACGCGCC 
CAGGAGATGT GCCAGAAGTC CGTTCCAGAA AACTGGAACC CTGACAGACC ATATCCTGAT 
GACCATCATG ACAGCCGCCA CAATCCACAG ACGGTAGAAA TGAGAGAGTT TGGGCCTGTT 
GGGGACGGTT ACTCGGACAC TGAGCCCTAT CACCCAATGG AAGGGCATGG CCGGACCATC 
TCCATGCCTC GCCTCTCTGC AGACAACCAA CGACGGAGAC ACAGGCCGCG AGGAAGTAAT 
CTCAGTACCA TCACCGACAC AAGTCCAATG CGTCGTTCTA CATCCTCATT GGTCCATGGA 
CGTTCAGGTC GAGGCGTGCG ATTGGATGAT TACTCTCTGG AACGGGTGGT GTCAGAAGAG 
GGTCACCGAC ATGCTCCCCG AAGGAGAGAT CGTGATCGAG ACCGGGATAG AGATCGCAGT 
CATCGAACCT CAGAGAGATC CCTCGCTCGC TATACAGATG CAGACACAGG TCTGGGTACA 
GATCTTAGCA CCACTACACA GTCGGGTGAC CTCCCACCTA AAGAAAGGGA ACGAGAACGG 
GGTCGAGCAA AAGACCGTCG CCACCACCAC CACCATCATC ACCACCACGG CTCGGTGGAT 
AAAGATCGGT ACAGTCACGA GCGCGACTAC CATCGTCATC CTCAAGACAG ATCAGATCGC 
CAATGGTCCC GCTCTCCGAG TGAGGGCAGA GGGCACAGAC AGGGCAGTAG TTCTGTGAGC 
GGAAGCCCCG TTCCCTCGAC GTCGGGGACC AGCACCCCCC GGCGTGGCCG TCGCCAGTTG 
CCCCAGACCC CATCCACTCC ACGCCCTCAC GTCACCTACT CCCCTGCGGT CCGCAAGCCT 
CCATATGGCT CCCCAGGCCA GGGTCGACTC CGCTCCCCTT CCCCGCGAAA CTTTTCTCCG 
CCAGGTCATG AACCGCCATA CCACCGTCCA CCCTCCCGCC ATGCCTCACC TCATCCTGGC 
TCACCCCGGC ATGCCTCTCC GCGTTCCCCC AGGCACACGT CGCCCAGGTC CCCTCGTCAC 
GGCCGCTGGG GCCCTCCGGA CAGCCTAGAA GGTGATGGAA CTTTCTACGA ACGGGACTAT 
GAGTACGAGC GCCACCATGA GCCGCCAGCC TACGAACAGA GTTTATCACA GGGCAACCCC 
CATCCACATT CACACCCTCA TCCCCGGTCA CCAAGGACTG CTCGTCACGG GCCTCAACCA 
CACCCGCGGC GTATGCCTAA CGGCTACCGT TCCTCCTCGC CCTCCCCGCA CCGGCGCGGC 
CCCCACCCAG GCCCACACCG GCCACCGCAT GGCCGTGGGC CCCGGAAGGG GTTGCACGAA 
CAATATAGCG AGACGGACGA GGATGATTGG TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213835.2
CDS	1436..8431
Translation	MARFGDEVPARYGGGGGGGSGQGGPGRGGSRQGGPPGAQRMYKQSMAQRARTMAIYNPIPVRQNCLTVNRSLFLFSEDNIVRKYAKKITEWPPFEWMILTTIIANCIVLALEQHLPVGDKTPLSERLEETEPYFIAIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSSVGSELDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGVLLFVAILMFAIIGLEFYMGKFHTTCFNTITGEMYDERPCGARTCDANNTQCQGYWLGPNYGITQFDNVLFAVLTVFQCITMEGWTDMLYYSNDALGSAWNWMYYVPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILAEDDNDTGSRRRPTLKKNKADLLNPQEGVDHMGDSVGSPFARGSMSGKGDETPFSKKERRLRFLIRKMVKTQAFYWTVISLVALNTLCVAVVHYDQPELLSDFLYYAEFIFLGLFMSEMLIKMYGLGTRPYFHSSFNCFDCAVIVGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFELGTPPTNFDTFPAAIMTVFQILTGEDWNVVMYDGIKSQGGVDKGMIFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEATTQKMALQKAKEVAEVSPLSAANLSIAAKEQQKNSKGSKSVWEQRTSEIRRQNLMTSREALYNELDAEDHWKMNYRNRPDVKTHLDRPLVVNPQENRNNNTNKPRPDDPNPQERSEEHPSRSHHHHSHHHHHHHHHTHHSSRQSLHLPELAETGFECDVEGAGEHRRPRHHQHRRRDEDGGERGRKHRDHHSRGETAGGDAEDGQEKRQRRHRHANHGDGDGRRERSRLHRNKKEGSLSTARPIQPGLPHTESQYSEDLDNLRNSSKMAIHDPDPDLYDPYYDPEQANNLLNLRGPDLHDSSLILTDSSKLEHTAIDMPPIYPSLNAKLQVNKNANTEPAKEGEKKEEEEDDGGDEEGHKRIPPFSSCFILSTTNPFRRCCHYILTLRYFEMCILSVIAMSSIALAAEDPVSQDSERNKVLRYFDYVFTGVFTFEMLIKMVVLGLILHEGSYFRDLWNFLDFVVVSGALVAFAFTGSGKGKDISIIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFARDCRGEYLVYERDNEVKSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPDVLKHSVDSTYENRGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYSIMSLIALNTIILMMKYNGASDAYDKVLKNLNIVFTTFFFMESILKIIAFGVRNFFRDAWNIFDFVSVIGSITDILVTELVNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAILFFIYAIIGMQLFGNLALDDEGAINEHNNFRTFFMALMLLFRSATGEAWHDIMLSCLGGMPCEPEEVGSECGSNVAYAYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQALREEQNRTPLMFQRMEPSPNQEGGPGLNGLPELPQEPVNSLPPEVGMNESQSWVTSRAQEMCQKSVPENWNPDRPYPDDHHDSRHNPQTVEMREFGPVGDGYSDTEPYHPMEGHGRTISMPRLSADNQRRRHRPRGSNLSTITDTSPMRRSTSSLVHGRSGRGVRLDDYSLERVVSEEGHRHAPRRRDRDRDRDRDRSHRTSERSLARYTDADTGLGTDLSTTTQSGDLPPKERERERGRAKDRRHHHHHHHHHGSVDKDRYSHERDYHRHPQDRSDRQWSRSPSEGRGHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPSTPRPHVTYSPAVRKPPYGSPGQGRLRSPSPRNFSPPGHEPPYHRPPSRHASPHPGSPRHASPRSPRHTSPRSPRHGRWGPPDSLEGDGTFYERDYEYERHHEPPAYEQSLSQGNPHPHSHPHPRSPRTARHGPQPHPRRMPNGYRSSSPSPHRRGPHPGPHRPPHGRGPRKGLHEQYSETDEDDWC

Target ORF information:

RefSeq Version	XM_017358346.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358346.2

ORF Insert Sequence:

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGGG GAGCGGTAAG GGGAAAGACA TCAGTATCAT CAAGTCTCTG 
AGGGTTCTAA GGGTGCTGCG TCCACTCAAG ACCATCAAAC GTCTTCCAAA ACTAAAAGCG 
GTGTTTGACT GTGTAGTAAA CTCTCTGAAG AATGTGTTGA ACATCCTCAT TGTCTACATG 
CTGTTCATGT TCATCTTCGC TGTGGTGGCC GTGCAGCTTT TTAAAGGACG ATTCTTTTAC 
TGCACTGATG AGTCTAAAGA GTTTGCCCGA GACTGCAGGG GCGAATATTT GGTGTACGAG 
CGCGATAACG AGGTCAAATC TCAGAAGAGG GAGTGGAAGA AGTACGATTT CCACTACGAC 
AATGTGCTTT GGGCCCTGCT TACTCTCTTT ACTGTGTCCA CCGGAGAGGG ATGGCCAGAT 
GTGTTGAAGC ACTCTGTGGA CTCCACTTAC GAGAATCGGG GCCCGAGCCC GGGATACAGG 
ATGGAAATGT CCATCTTTTA CGTGGTGTAC TTCGTTGTCT TCCCCTTTTT CTTCGTAAAC 
ATATTTGTAG CTCTCATCAT CATCACCTTC CAAGAGCAAG GAGACAAGAT GATGGAGGAC 
TACAGCCTCG AAAAAAATGA GAGAGCCTGT ATTGATTTTG CTATAAATGC CAAACCTCTA 
ACGCGCCACA TGCCTCAGAA CAAGCAAAGC TTTCAGTACC GAATGTGGCA GTTTGTGGTG 
TCGCCTCCGT TTGAGTACAG TATCATGTCT CTCATTGCGC TCAACACCAT CATCCTTATG 
ATGAAGTATA ATGGGGCTTC CGATGCATAT GACAAAGTCC TGAAGAACCT GAATATAGTG 
TTCACCACAT TCTTCTTCAT GGAGAGTATT CTGAAAATCA TTGCCTTTGG TGTCAGAAAT 
TTTTTCAGAG ATGCTTGGAA TATTTTTGAT TTTGTGTCTG TTATCGGCAG CATCACTGAT 
ATTTTAGTGA CTGAGCTCGT GAATAACTTC ATAAACCTGA GTTTTCTGAG ACTCTTTCGA 
GCAGCTCGTC TCATTAAGCT CTTGCGGCAG GGCGAGACCA TTCGCATTCT GCTTTGGACC 
TTCGTTCAGT CATTTAAGGC TCTGCCATAT GTATGCCTGC TCATTGCAAT TCTGTTTTTC 
ATCTACGCCA TCATTGGCAT GCAGCTGTTT GGGAACTTGG CTCTGGATGA CGAAGGTGCT 
ATCAATGAAC ACAACAACTT CAGAACCTTC TTCATGGCCC TCATGCTCCT CTTCAGGAGT 
GCCACGGGCG AGGCGTGGCA TGATATCATG CTGTCATGTC TGGGAGGAAT GCCCTGTGAG 
CCGGAGGAGG TTGGATCAGA ATGTGGCAGC AATGTGGCCT ATGCATACTT CGTCTCTTTC 
ATCTTTCTCT GTTCGTTCCT GATGCTGAAT CTTTTTGTGG CTGTCATCAT GGATAACTTT 
GAGTACTTGA CCAGGGACTC CTCCATCCTG GGTCCGCATC ATTTGGATGA GTATGTCAGG 
ATATGGGCAG AGTACGACCC CGCAGCCTGC GGGCGCATTC ATTATAAGGA TATGTACAGT 
TTATTGCGGG TTATCGACCC CCCGCTGGGC TTAGGCAAGA AATGTCCTCA TAGGGTGGCC 
TGCAAGAGAC TGCTCCGTAT GGACCTGCCC GTTGCTGATG ACAACACGGT GCATTTCAAC 
TCCACTCTGA TGGCCTTGAT TCGCACAGCA CTGGACATCA AAATTGCCAA GGGAGGAGCG 
GACAAACACC AGATGGACGC GGAGCTCCGT AAAGAAATGA TGGCGATTTG GCCAAATCTC 
TCTCAGAAGA ACCTGGATCT GCTGGTAACG CCACACAAGT CGACAGACCT GACAGTGGGC 
AAGATCTACG CCGCCATGAT GATCATGGAG TACTACCGGC AGAGCAAGGC CAAAAAGCTG 
CAGGCGCTTC GTGAGGAGCA GAACCGAACG CCATTAATGT TTCAGCGCAT GGAGCCGTCC 
CCAAACCAGG AAGGGGGACC GGGGCTGAAT GGCCTGCCCG AGCTGCCGCA GGAGCCCGTT 
AACAGCCTGC CTCCAGAGGT TGGAATGAAT GAAAGCCAGT CATGGGTGAC GTCACGCGCC 
CAGGAGATGT GCCAGAAGTC CGTTCCAGAA AACTGGAACC CTGACAGACC ATATCCTGAT 
GACCATCATG ACAGCCGCCA CAATCCACAG ACGGTAGAAA TGAGAGAGTT TGGGCCTGTT 
GGGGACGGTT ACTCGGACAC TGAGCCCTAT CACCCAATGG AAGGGCATGG CCGGACCATC 
TCCATGCCTC GCCTCTCTGC AGACAACCAA CGACGGAGAC ACAGGCCGCG AGGAAGTAAT 
CTCAGTACCA TCACCGACAC AAGTCCAATG CGTCGTTCTA CATCCTCATT GGTCCATGGA 
CGTTCAGGTC GAGGCGTGCG ATTGGATGAT TACTCTCTGG AACGGGTGGT GTCAGAAGAG 
GGTCACCGAC ATGCTCCCCG AAGGAGAGAT CGTGATCGAG ACCGGGATAG AGATCGCAGT 
CATCGAACCT CAGAGAGATC CCTCGCTCGC TATACAGATG CAGACACAGG TCTGGGTACA 
GATCTTAGCA CCACTACACA GTCGGGTGAC CTCCCACCTA AAGAAAGGGA ACGAGAACGG 
GGTCGAGCAA AAGACCGTCG CCACCACCAC CACCATCATC ACCACCACGG CTCGGTGGAT 
AAAGATCGGT ACAGTCACGA GCGCGACTAC CATCGTCATC CTCAAGACAG ATCAGATCGC 
CAATGGTCCC GCTCTCCGAG TGAGGGCAGA GGGCACAGAC AGGGCAGTAG TTCTGTGAGC 
GGAAGCCCCG TTCCCTCGAC GTCGGGGACC AGCACCCCCC GGCGTGGCCG TCGCCAGTTG 
CCCCAGACCC CATCCACTCC ACGCCCTCAC GTCACCTACT CCCCTGCGGT CCGCAAGCCT 
CCATATGGCT CCCCAGGCCA GGGTCGACTC CGCTCCCCTT CCCCGCGAAA CTTTTCTCCG 
CCAGGTCATG AACCGCCATA CCACCGTCCA CCCTCCCGCC ATGCCTCACC TCATCCTGGC 
TCACCCCGGC ATGCCTCTCC GCGTTCCCCC AGGCACACGT CGCCCAGGTC CCCTCGTCAC 
GGCCGCTGGG GCCCTCCGGA CAGCCTAGAA GGTGATGGAA CTTTCTACGA ACGGGACTAT 
GAGTACGAGC GCCACCATGA GCCGCCAGCC TACGAACAGA GTTTATCACA GGGCAACCCC 
CATCCACATT CACACCCTCA TCCCCGGTCA CCAAGGACTG CTCGTCACGG GCCTCAACCA 
CACCCGCGGC GTATGCCTAA CGGCTACCGT TCCTCCTCGC CCTCCCCGCA CCGGCGCGGC 
CCCCACCCAG GCCCACACCG GCCACCGCAT GGCCGTGGGC CCCGGAAGGG GTTGCACGAA 
CAATATAGCG AGACGGACGA GGATGATTGG TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ab ( XM_021479649.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335324.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X9, mRNA.

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CloneID	ODa147901
Clone ID Related Accession (Same CDS sequence)	XM_021479649.1
Accession Version	XM_021479649.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGGG GAGCGGTAAG GGGAAAGACA TCAGTATCAT CAAGTCTCTG 
AGGGTTCTAA GGGTGCTGCG TCCACTCAAG ACCATCAAAC GTCTTCCAAA ACTAAAAGCG 
GTGTTTGACT GTGTAGTAAA CTCTCTGAAG AATGTGTTGA ACATCCTCAT TGTCTACATG 
CTGTTCATGT TCATCTTCGC TGTGGTGGCC GTGCAGCTTT TTAAAGGACG ATTCTTTTAC 
TGCACTGATG AGTCTAAAGA GTTTGCCCGA GACTGCAGGG GCGAATATTT GGTGTACGAG 
CGCGATAACG AGGTCAAATC TCAGAAGAGG GAGTGGAAGA AGTACGATTT CCACTACGAC 
AATGTGCTTT GGGCCCTGCT TACTCTCTTT ACTGTGTCCA CCGGAGAGGG ATGGCCAGAT 
GTGTTGAAGC ACTCTGTGGA CTCCACTTAC GAGAATCGGG GCCCGAGCCC GGGATACAGG 
ATGGAAATGT CCATCTTTTA CGTGGTGTAC TTCGTTGTCT TCCCCTTTTT CTTCGTAAAC 
ATATTTGTAG CTCTCATCAT CATCACCTTC CAAGAGCAAG GAGACAAGAT GATGGAGGAC 
TACAGCCTCG AAAAAAATGA GAGAGCCTGT ATTGATTTTG CTATAAATGC CAAACCTCTA 
ACGCGCCACA TGCCTCAGAA CAAGCAAAGC TTTCAGTACC GAATGTGGCA GTTTGTGGTG 
TCGCCTCCGT TTGAGTACAG TATCATGTCT CTCATTGCGC TCAACACCAT CATCCTTATG 
ATGAAGTATA ATGGGGCTTC CGATGCATAT GACAAAGTCC TGAAGAACCT GAATATAGTG 
TTCACCACAT TCTTCTTCAT GGAGAGTATT CTGAAAATCA TTGCCTTTGG TGTCAGAAAT 
TTTTTCAGAG ATGCTTGGAA TATTTTTGAT TTTGTGTCTG TTATCGGCAG CATCACTGAT 
ATTTTAGTGA CTGAGCTCGT GAATAACTTC ATAAACCTGA GTTTTCTGAG ACTCTTTCGA 
GCAGCTCGTC TCATTAAGCT CTTGCGGCAG GGCGAGACCA TTCGCATTCT GCTTTGGACC 
TTCGTTCAGT CATTTAAGGC TCTGCCATAT GTATGCCTGC TCATTGCAAT TCTGTTTTTC 
ATCTACGCCA TCATTGGCAT GCAGCTGTTT GGGAACTTGG CTCTGGATGA CGAAGGTGCT 
ATCAATGAAC ACAACAACTT CAGAACCTTC TTCATGGCCC TCATGCTCCT CTTCAGGAGT 
GCCACGGGCG AGGCGTGGCA TGATATCATG CTGTCATGTC TGGGAGGAAT GCCCTGTGAG 
CCGGAGGAGG TTGGATCAGA ATGTGGCAGC AATGTGGCCT ATGCATACTT CGTCTCTTTC 
ATCTTTCTCT GTTCGTTCCT GATGCTGAAT CTTTTTGTGG CTGTCATCAT GGATAACTTT 
GAGTACTTGA CCAGGGACTC CTCCATCCTG GGTCCGCATC ATTTGGATGA GTATGTCAGG 
ATATGGGCAG AGTACGACCC CGCAGCCTGC GGCAGGATCA GTTACAGTGA TATGTATGAG 
ATGCTCAGGC ACATGTGTCC TCCGCTAGGC CTGGGGAAGA GGTGCCCTGC TCGGGTCGCC 
TACAAGAGAC TGCTCCGTAT GGACCTGCCC GTTGCTGATG ACAACACGGT GCATTTCAAC 
TCCACTCTGA TGGCCTTGAT TCGCACAGCA CTGGACATCA AAATTGCCAA GGGAGGAGCG 
GACAAACACC AGATGGACGC GGAGCTCCGT AAAGAAATGA TGGCGATTTG GCCAAATCTC 
TCTCAGAAGA ACCTGGATCT GCTGGTAACG CCACACAAGT CGACAGACCT GACAGTGGGC 
AAGATCTACG CCGCCATGAT GATCATGGAG TACTACCGGC AGAGCAAGGC CAAAAAGCTG 
CAGGCGCTTC GTGAGGAGCA GAACCGAACG CCATTAATGT TTCAGCGCAT GGAGCCGTCC 
CCAAACCAGG AAGGGGGACC GGGGCTGAAT GGCCTGCCCG AGCTGCCGCA GGAGCCCGTT 
AACAGCCTGC CTCCAGAGGT TGGAATGAAT GAAAGCCAGT CATGGGTGAC GTCACGCGCC 
CAGGAGATGT GCCAGAAGTC CGTTCCAGAA AACTGGAACC CTGACAGACC ATATCCTGAT 
GACCATCATG ACAGCCGCCA CAATCCACAG ACGGTAGAAA TGAGAGAGTT TGGGCCTGTT 
GGGGACGGTT ACTCGGACAC TGAGCCCTAT CACCCAATGG AAGGGCATGG CCGGACCATC 
TCCATGCCTC GCCTCTCTGC AGACAACCAA CGACGGAGAC ACAGGCCGCG AGGAAGTAAT 
CTCAGTACCA TCACCGACAC AAGTCCAATG CGTCGTTCTA CATCCTCATT GGTCCATGGA 
CGTTCAGGTC GAGGCGTGCG ATTGGATGAT TACTCTCTGG AACGGGTGGT GTCAGAAGAG 
GGTCACCGAC ATGCTCCCCG AAGGAGAGAT CGTGATCGAG ACCGGGATAG AGATCGCAGT 
CATCGAACCT CAGAGAGATC CCTCGCTCGC TATACAGATG CAGACACAGG TCTGGGTACA 
GATCTTAGCA CCACTACACA GTCGGGTGAC CTCCCACCTA AAGAAAGGGA ACGAGAACGG 
GGTCGAGCAA AAGACCGTCG CCACCACCAC CACCATCATC ACCACCACGG CTCGGTGGAT 
AAAGATCGGT ACAGTCACGA GCGCGACTAC CATCGTCATC CTCAAGACAG ATCAGATCGC 
CAATGGTCCC GCTCTCCGAG TGAGGGCAGA GGGCACAGAC AGGGCAGTAG TTCTGTGAGC 
GGAAGCCCCG TTCCCTCGAC GTCGGGGACC AGCACCCCCC GGCGTGGCCG TCGCCAGTTG 
CCCCAGACCC CATCCACTCC ACGCCCTCAC GTCACCTACT CCCCTGCGGT CCGCAAGCCT 
CCATATGGCT CCCCAGGCCA GGGTCGACTC CGCTCCCCTT CCCCGCGAAA CTTTTCTCCG 
CCAGGTCATG AACCGCCATA CCACCGTCCA CCCTCCCGCC ATGCCTCACC TCATCCTGGC 
TCACCCCGGC ATGCCTCTCC GCGTTCCCCC AGGCACACGT CGCCCAGGTC CCCTCGTCAC 
GGCCGCTGGG GCCCTCCGGA CAGCCTAGAA GGTGATGGAA CTTTCTACGA ACGGGACTAT 
GAGTACGAGC GCCACCATGA GCCGCCAGCC TACGAACAGA GTTTATCACA GGGCAACCCC 
CATCCACATT CACACCCTCA TCCCCGGTCA CCAAGGACTG CTCGTCACGG GCCTCAACCA 
CACCCGCGGC GTATGCCTAA CGGCTACCGT TCCTCCTCGC CCTCCCCGCA CCGGCGCGGC 
CCCCACCCAG GCCCACACCG GCCACCGCAT GGCCGTGGGC CCCGGAAGGG GTTGCACGAA 
CAATATAGCG AGACGGACGA GGATGATTGG TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335324.1
CDS	1436..8431
Translation	MARFGDEVPARYGGGGGGGSGQGGPGRGGSRQGGPPGAQRMYKQSMAQRARTMAIYNPIPVRQNCLTVNRSLFLFSEDNIVRKYAKKITEWPPFEWMILTTIIANCIVLALEQHLPVGDKTPLSERLEETEPYFIAIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSSVGSELDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGVLLFVAILMFAIIGLEFYMGKFHTTCFNTITGEMYDERPCGARTCDANNTQCQGYWLGPNYGITQFDNVLFAVLTVFQCITMEGWTDMLYYSNDALGSAWNWMYYVPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILAEDDNDTGSRRRPTLKKNKADLLNPQEGVDHMGDSVGSPFARGSMSGKGDETPFSKKERRLRFLIRKMVKTQAFYWTVISLVALNTLCVAVVHYDQPELLSDFLYYAEFIFLGLFMSEMLIKMYGLGTRPYFHSSFNCFDCAVIVGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFELGTPPTNFDTFPAAIMTVFQILTGEDWNVVMYDGIKSQGGVDKGMIFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEATTQKMALQKAKEVAEVSPLSAANLSIAAKEQQKNSKGSKSVWEQRTSEIRRQNLMTSREALYNELDAEDHWKMNYRNRPDVKTHLDRPLVVNPQENRNNNTNKPRPDDPNPQERSEEHPSRSHHHHSHHHHHHHHHTHHSSRQSLHLPELAETGFECDVEGAGEHRRPRHHQHRRRDEDGGERGRKHRDHHSRGETAGGDAEDGQEKRQRRHRHANHGDGDGRRERSRLHRNKKEGSLSTARPIQPGLPHTESQYSEDLDNLRNSSKMAIHDPDPDLYDPYYDPEQANNLLNLRGPDLHDSSLILTDSSKLEHTAIDMPPIYPSLNAKLQVNKNANTEPAKEGEKKEEEEDDGGDEEGHKRIPPFSSCFILSTTNPFRRCCHYILTLRYFEMCILSVIAMSSIALAAEDPVSQDSERNKVLRYFDYVFTGVFTFEMLIKMVVLGLILHEGSYFRDLWNFLDFVVVSGALVAFAFTGSGKGKDISIIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFARDCRGEYLVYERDNEVKSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPDVLKHSVDSTYENRGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYSIMSLIALNTIILMMKYNGASDAYDKVLKNLNIVFTTFFFMESILKIIAFGVRNFFRDAWNIFDFVSVIGSITDILVTELVNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAILFFIYAIIGMQLFGNLALDDEGAINEHNNFRTFFMALMLLFRSATGEAWHDIMLSCLGGMPCEPEEVGSECGSNVAYAYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRISYSDMYEMLRHMCPPLGLGKRCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQALREEQNRTPLMFQRMEPSPNQEGGPGLNGLPELPQEPVNSLPPEVGMNESQSWVTSRAQEMCQKSVPENWNPDRPYPDDHHDSRHNPQTVEMREFGPVGDGYSDTEPYHPMEGHGRTISMPRLSADNQRRRHRPRGSNLSTITDTSPMRRSTSSLVHGRSGRGVRLDDYSLERVVSEEGHRHAPRRRDRDRDRDRDRSHRTSERSLARYTDADTGLGTDLSTTTQSGDLPPKERERERGRAKDRRHHHHHHHHHGSVDKDRYSHERDYHRHPQDRSDRQWSRSPSEGRGHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPSTPRPHVTYSPAVRKPPYGSPGQGRLRSPSPRNFSPPGHEPPYHRPPSRHASPHPGSPRHASPRSPRHTSPRSPRHGRWGPPDSLEGDGTFYERDYEYERHHEPPAYEQSLSQGNPHPHSHPHPRSPRTARHGPQPHPRRMPNGYRSSSPSPHRRGPHPGPHRPPHGRGPRKGLHEQYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021479649.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479649.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGGG GAGCGGTAAG GGGAAAGACA TCAGTATCAT CAAGTCTCTG 
AGGGTTCTAA GGGTGCTGCG TCCACTCAAG ACCATCAAAC GTCTTCCAAA ACTAAAAGCG 
GTGTTTGACT GTGTAGTAAA CTCTCTGAAG AATGTGTTGA ACATCCTCAT TGTCTACATG 
CTGTTCATGT TCATCTTCGC TGTGGTGGCC GTGCAGCTTT TTAAAGGACG ATTCTTTTAC 
TGCACTGATG AGTCTAAAGA GTTTGCCCGA GACTGCAGGG GCGAATATTT GGTGTACGAG 
CGCGATAACG AGGTCAAATC TCAGAAGAGG GAGTGGAAGA AGTACGATTT CCACTACGAC 
AATGTGCTTT GGGCCCTGCT TACTCTCTTT ACTGTGTCCA CCGGAGAGGG ATGGCCAGAT 
GTGTTGAAGC ACTCTGTGGA CTCCACTTAC GAGAATCGGG GCCCGAGCCC GGGATACAGG 
ATGGAAATGT CCATCTTTTA CGTGGTGTAC TTCGTTGTCT TCCCCTTTTT CTTCGTAAAC 
ATATTTGTAG CTCTCATCAT CATCACCTTC CAAGAGCAAG GAGACAAGAT GATGGAGGAC 
TACAGCCTCG AAAAAAATGA GAGAGCCTGT ATTGATTTTG CTATAAATGC CAAACCTCTA 
ACGCGCCACA TGCCTCAGAA CAAGCAAAGC TTTCAGTACC GAATGTGGCA GTTTGTGGTG 
TCGCCTCCGT TTGAGTACAG TATCATGTCT CTCATTGCGC TCAACACCAT CATCCTTATG 
ATGAAGTATA ATGGGGCTTC CGATGCATAT GACAAAGTCC TGAAGAACCT GAATATAGTG 
TTCACCACAT TCTTCTTCAT GGAGAGTATT CTGAAAATCA TTGCCTTTGG TGTCAGAAAT 
TTTTTCAGAG ATGCTTGGAA TATTTTTGAT TTTGTGTCTG TTATCGGCAG CATCACTGAT 
ATTTTAGTGA CTGAGCTCGT GAATAACTTC ATAAACCTGA GTTTTCTGAG ACTCTTTCGA 
GCAGCTCGTC TCATTAAGCT CTTGCGGCAG GGCGAGACCA TTCGCATTCT GCTTTGGACC 
TTCGTTCAGT CATTTAAGGC TCTGCCATAT GTATGCCTGC TCATTGCAAT TCTGTTTTTC 
ATCTACGCCA TCATTGGCAT GCAGCTGTTT GGGAACTTGG CTCTGGATGA CGAAGGTGCT 
ATCAATGAAC ACAACAACTT CAGAACCTTC TTCATGGCCC TCATGCTCCT CTTCAGGAGT 
GCCACGGGCG AGGCGTGGCA TGATATCATG CTGTCATGTC TGGGAGGAAT GCCCTGTGAG 
CCGGAGGAGG TTGGATCAGA ATGTGGCAGC AATGTGGCCT ATGCATACTT CGTCTCTTTC 
ATCTTTCTCT GTTCGTTCCT GATGCTGAAT CTTTTTGTGG CTGTCATCAT GGATAACTTT 
GAGTACTTGA CCAGGGACTC CTCCATCCTG GGTCCGCATC ATTTGGATGA GTATGTCAGG 
ATATGGGCAG AGTACGACCC CGCAGCCTGC GGCAGGATCA GTTACAGTGA TATGTATGAG 
ATGCTCAGGC ACATGTGTCC TCCGCTAGGC CTGGGGAAGA GGTGCCCTGC TCGGGTCGCC 
TACAAGAGAC TGCTCCGTAT GGACCTGCCC GTTGCTGATG ACAACACGGT GCATTTCAAC 
TCCACTCTGA TGGCCTTGAT TCGCACAGCA CTGGACATCA AAATTGCCAA GGGAGGAGCG 
GACAAACACC AGATGGACGC GGAGCTCCGT AAAGAAATGA TGGCGATTTG GCCAAATCTC 
TCTCAGAAGA ACCTGGATCT GCTGGTAACG CCACACAAGT CGACAGACCT GACAGTGGGC 
AAGATCTACG CCGCCATGAT GATCATGGAG TACTACCGGC AGAGCAAGGC CAAAAAGCTG 
CAGGCGCTTC GTGAGGAGCA GAACCGAACG CCATTAATGT TTCAGCGCAT GGAGCCGTCC 
CCAAACCAGG AAGGGGGACC GGGGCTGAAT GGCCTGCCCG AGCTGCCGCA GGAGCCCGTT 
AACAGCCTGC CTCCAGAGGT TGGAATGAAT GAAAGCCAGT CATGGGTGAC GTCACGCGCC 
CAGGAGATGT GCCAGAAGTC CGTTCCAGAA AACTGGAACC CTGACAGACC ATATCCTGAT 
GACCATCATG ACAGCCGCCA CAATCCACAG ACGGTAGAAA TGAGAGAGTT TGGGCCTGTT 
GGGGACGGTT ACTCGGACAC TGAGCCCTAT CACCCAATGG AAGGGCATGG CCGGACCATC 
TCCATGCCTC GCCTCTCTGC AGACAACCAA CGACGGAGAC ACAGGCCGCG AGGAAGTAAT 
CTCAGTACCA TCACCGACAC AAGTCCAATG CGTCGTTCTA CATCCTCATT GGTCCATGGA 
CGTTCAGGTC GAGGCGTGCG ATTGGATGAT TACTCTCTGG AACGGGTGGT GTCAGAAGAG 
GGTCACCGAC ATGCTCCCCG AAGGAGAGAT CGTGATCGAG ACCGGGATAG AGATCGCAGT 
CATCGAACCT CAGAGAGATC CCTCGCTCGC TATACAGATG CAGACACAGG TCTGGGTACA 
GATCTTAGCA CCACTACACA GTCGGGTGAC CTCCCACCTA AAGAAAGGGA ACGAGAACGG 
GGTCGAGCAA AAGACCGTCG CCACCACCAC CACCATCATC ACCACCACGG CTCGGTGGAT 
AAAGATCGGT ACAGTCACGA GCGCGACTAC CATCGTCATC CTCAAGACAG ATCAGATCGC 
CAATGGTCCC GCTCTCCGAG TGAGGGCAGA GGGCACAGAC AGGGCAGTAG TTCTGTGAGC 
GGAAGCCCCG TTCCCTCGAC GTCGGGGACC AGCACCCCCC GGCGTGGCCG TCGCCAGTTG 
CCCCAGACCC CATCCACTCC ACGCCCTCAC GTCACCTACT CCCCTGCGGT CCGCAAGCCT 
CCATATGGCT CCCCAGGCCA GGGTCGACTC CGCTCCCCTT CCCCGCGAAA CTTTTCTCCG 
CCAGGTCATG AACCGCCATA CCACCGTCCA CCCTCCCGCC ATGCCTCACC TCATCCTGGC 
TCACCCCGGC ATGCCTCTCC GCGTTCCCCC AGGCACACGT CGCCCAGGTC CCCTCGTCAC 
GGCCGCTGGG GCCCTCCGGA CAGCCTAGAA GGTGATGGAA CTTTCTACGA ACGGGACTAT 
GAGTACGAGC GCCACCATGA GCCGCCAGCC TACGAACAGA GTTTATCACA GGGCAACCCC 
CATCCACATT CACACCCTCA TCCCCGGTCA CCAAGGACTG CTCGTCACGG GCCTCAACCA 
CACCCGCGGC GTATGCCTAA CGGCTACCGT TCCTCCTCGC CCTCCCCGCA CCGGCGCGGC 
CCCCACCCAG GCCCACACCG GCCACCGCAT GGCCGTGGGC CCCGGAAGGG GTTGCACGAA 
CAATATAGCG AGACGGACGA GGATGATTGG TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ab ( XM_021479650.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335325.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X10, mRNA.

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CloneID	ODa147902
Clone ID Related Accession (Same CDS sequence)	XM_021479650.1
Accession Version	XM_021479650.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGGCGCAT TCATTATAAG 
GATATGTACA GTTTATTGCG GGTTATCGAC CCCCCGCTGG GCTTAGGCAA GAAATGTCCT 
CATAGGGTGG CCTGCAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCAGCGACA 
GACCTGACAG TGGGCAAGAT CTACGCCGCC ATGATGATCA TGGAGTACTA CCGGCAGAGC 
AAGGCCAAAA AGCTGCAGGC GCTTCGTGAG GAGCAGAACC GAACGCCATT AATGTTTCAG 
CGCATGGAGC CGTCCCCAAA CCAGGAAGGG GGACCGGGGC TGAATGGCCT GCCCGAGCTG 
CCGCAGGAGC CCGTTAACAG CCTGCCTCCA GAGGTTGGAA TGAATGAAAG CCAGTCATGG 
GTGACGTCAC GCGCCCAGGA GATGTGCCAG AAGTCCGTTC CAGAAAACTG GAACCCTGAC 
AGACCATATC CTGATGACCA TCATGACAGC CGCCACAATC CACAGACGGT AGAAATGAGA 
GAGTTTGGGC CTGTTGGGGA CGGTTACTCG GACACTGAGC CCTATCACCC AATGGAAGGG 
CATGGCCGGA CCATCTCCAT GCCTCGCCTC TCTGCAGACA ACCAAAGTGT GCGTTCTGCT 
GCGACCATCA CCGACACAAG TCCAATGCGT CGTTCTACAT CCTCATTGGT CCATGGACGT 
TCAGGTCGAG GCGTGCGATT GGATGATTAC TCTCTGGAAC GGGTGGTGTC AGAAGAGGGT 
CACCGACATG CTCCCCGAAG GAGAGATCGT GATCGAGACC GGGATAGAGA TCGCAGTCAT 
CGAACCTCAG AGAGATCCCT CGCTCGCTAT ACAGATGCAG ACACAGGTCT GGGTACAGAT 
CTTAGCACCA CTACACAGTC GGGTGACCTC CCACCTAAAG AAAGGGAACG AGAACGGGGT 
CGAGCAAAAG ACCGTCGCCA CCACCACCAC CATCATCACC ACCACGGCTC GGTGGATAAA 
GATCGGTACA GTCACGAGCG CGACTACCAT CGTCATCCTC AAGACAGATC AGATCGCCAA 
TGGTCCCGCT CTCCGAGTGA GGGCAGAGGG CACAGACAGG GCAGTAGTTC TGTGAGCGGA 
AGCCCCGTTC CCTCGACGTC GGGGACCAGC ACCCCCCGGC GTGGCCGTCG CCAGTTGCCC 
CAGACCCCAT CCACTCCACG CCCTCACGTC ACCTACTCCC CTGCGGTCCG CAAGCCTCCA 
TATGGCTCCC CAGGCCAGGG TCGACTCCGC TCCCCTTCCC CGCGAAACTT TTCTCCGCCA 
GGTCATGAAC CGCCATACCA CCGTCCACCC TCCCGCCATG CCTCACCTCA TCCTGGCTCA 
CCCCGGCATG CCTCTCCGCG TTCCCCCAGG CACACGTCGC CCAGGTCCCC TCGTCACGGC 
CGCTGGGGCC CTCCGGACAG CCTAGAAGGT GATGGAACTT TCTACGAACG GGACTATGAG 
TACGAGCGCC ACCATGAGCC GCCAGCCTAC GAACAGAGTT TATCACAGGG CAACCCCCAT 
CCACATTCAC ACCCTCATCC CCGGTCACCA AGGACTGCTC GTCACGGGCC TCAACCACAC 
CCGCGGCGTA TGCCTAACGG CTACCGTTCC TCCTCGCCCT CCCCGCACCG GCGCGGCCCC 
CACCCAGGCC CACACCGGCC ACCGCATGGC CGTGGGCCCC GGAAGGGGTT GCACGAACAA 
TATAGCGAGA CGGACGAGGA TGATTGGTGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335325.1
CDS	1436..8428
Translation	MARFGDEVPARYGGGGGGGSGQGGPGRGGSRQGGPPGAQRMYKQSMAQRARTMAIYNPIPVRQNCLTVNRSLFLFSEDNIVRKYAKKITEWPPFEWMILTTIIANCIVLALEQHLPVGDKTPLSERLEETEPYFIAIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSSVGSELDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGVLLFVAILMFAIIGLEFYMGKFHTTCFNTITGEMYDERPCGARTCDANNTQCQGYWLGPNYGITQFDNVLFAVLTVFQCITMEGWTDMLYYSNDALGSAWNWMYYVPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILAEDDNDTGSRRRPTLKKNKADLLNPQEGVDHMGDSVGSPFARGSMSGKGDETPFSKKERRLRFLIRKMVKTQAFYWTVISLVALNTLCVAVVHYDQPELLSDFLYYAEFIFLGLFMSEMLIKMYGLGTRPYFHSSFNCFDCAVIVGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFELGTPPTNFDTFPAAIMTVFQILTGEDWNVVMYDGIKSQGGVDKGMIFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEATTQKMALQKAKEVAEVSPLSAANLSIAAKEQQKNSKGSKSVWEQRTSEIRRQNLMTSREALYNELDAEDHWKMNYRNRPDVKTHLDRPLVVNPQENRNNNTNKPRPDDPNPQERSEEHPSRSHHHHSHHHHHHHHHTHHSSRQSLHLPELAETGFECDVEGAGEHRRPRHHQHRRRDEDGGERGRKHRDHHSRGETAGGDAEDGQEKRQRRHRHANHGDGDGRRERSRLHRNKKEGSLSTARPIQPGLPHTESQYSEDLDNLRNSSKMAIHDPDPDLYDPYYDPEQANNLLNLRGPDLHDSSLILTDSSKLEHTAIDMPPIYPSLNAKLQVNKNANTEPAKEGEKKEEEEDDGGDEEGHKRIPPFSSCFILSTTNPFRRCCHYILTLRYFEMCILSVIAMSSIALAAEDPVSQDSERNKVLRYFDYVFTGVFTFEMLIKMVVLGLILHEGSYFRDLWNFLDFVVVSGALVAFAFTSFSGGSGKGKDISIIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFARDCRGEYLVYERDNEVKSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPDVLKHSVDSTYENRGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYSIMSLIALNTIILMMKYNGASDAYDKVLKNLNIVFTTFFFMESILKIIAFGVRNFFRDAWNIFDFVSVIGSITDILVTELVNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAILFFIYAIIGMQLFGNLALDDEGAINEHNNFRTFFMALMLLFRSATGEAWHDIMLSCLGGMPCEPEEVGSECGSNVAYAYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKKLQALREEQNRTPLMFQRMEPSPNQEGGPGLNGLPELPQEPVNSLPPEVGMNESQSWVTSRAQEMCQKSVPENWNPDRPYPDDHHDSRHNPQTVEMREFGPVGDGYSDTEPYHPMEGHGRTISMPRLSADNQSVRSAATITDTSPMRRSTSSLVHGRSGRGVRLDDYSLERVVSEEGHRHAPRRRDRDRDRDRDRSHRTSERSLARYTDADTGLGTDLSTTTQSGDLPPKERERERGRAKDRRHHHHHHHHHGSVDKDRYSHERDYHRHPQDRSDRQWSRSPSEGRGHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPSTPRPHVTYSPAVRKPPYGSPGQGRLRSPSPRNFSPPGHEPPYHRPPSRHASPHPGSPRHASPRSPRHTSPRSPRHGRWGPPDSLEGDGTFYERDYEYERHHEPPAYEQSLSQGNPHPHSHPHPRSPRTARHGPQPHPRRMPNGYRSSSPSPHRRGPHPGPHRPPHGRGPRKGLHEQYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021479650.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479650.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGGCGCAT TCATTATAAG 
GATATGTACA GTTTATTGCG GGTTATCGAC CCCCCGCTGG GCTTAGGCAA GAAATGTCCT 
CATAGGGTGG CCTGCAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCAGCGACA 
GACCTGACAG TGGGCAAGAT CTACGCCGCC ATGATGATCA TGGAGTACTA CCGGCAGAGC 
AAGGCCAAAA AGCTGCAGGC GCTTCGTGAG GAGCAGAACC GAACGCCATT AATGTTTCAG 
CGCATGGAGC CGTCCCCAAA CCAGGAAGGG GGACCGGGGC TGAATGGCCT GCCCGAGCTG 
CCGCAGGAGC CCGTTAACAG CCTGCCTCCA GAGGTTGGAA TGAATGAAAG CCAGTCATGG 
GTGACGTCAC GCGCCCAGGA GATGTGCCAG AAGTCCGTTC CAGAAAACTG GAACCCTGAC 
AGACCATATC CTGATGACCA TCATGACAGC CGCCACAATC CACAGACGGT AGAAATGAGA 
GAGTTTGGGC CTGTTGGGGA CGGTTACTCG GACACTGAGC CCTATCACCC AATGGAAGGG 
CATGGCCGGA CCATCTCCAT GCCTCGCCTC TCTGCAGACA ACCAAAGTGT GCGTTCTGCT 
GCGACCATCA CCGACACAAG TCCAATGCGT CGTTCTACAT CCTCATTGGT CCATGGACGT 
TCAGGTCGAG GCGTGCGATT GGATGATTAC TCTCTGGAAC GGGTGGTGTC AGAAGAGGGT 
CACCGACATG CTCCCCGAAG GAGAGATCGT GATCGAGACC GGGATAGAGA TCGCAGTCAT 
CGAACCTCAG AGAGATCCCT CGCTCGCTAT ACAGATGCAG ACACAGGTCT GGGTACAGAT 
CTTAGCACCA CTACACAGTC GGGTGACCTC CCACCTAAAG AAAGGGAACG AGAACGGGGT 
CGAGCAAAAG ACCGTCGCCA CCACCACCAC CATCATCACC ACCACGGCTC GGTGGATAAA 
GATCGGTACA GTCACGAGCG CGACTACCAT CGTCATCCTC AAGACAGATC AGATCGCCAA 
TGGTCCCGCT CTCCGAGTGA GGGCAGAGGG CACAGACAGG GCAGTAGTTC TGTGAGCGGA 
AGCCCCGTTC CCTCGACGTC GGGGACCAGC ACCCCCCGGC GTGGCCGTCG CCAGTTGCCC 
CAGACCCCAT CCACTCCACG CCCTCACGTC ACCTACTCCC CTGCGGTCCG CAAGCCTCCA 
TATGGCTCCC CAGGCCAGGG TCGACTCCGC TCCCCTTCCC CGCGAAACTT TTCTCCGCCA 
GGTCATGAAC CGCCATACCA CCGTCCACCC TCCCGCCATG CCTCACCTCA TCCTGGCTCA 
CCCCGGCATG CCTCTCCGCG TTCCCCCAGG CACACGTCGC CCAGGTCCCC TCGTCACGGC 
CGCTGGGGCC CTCCGGACAG CCTAGAAGGT GATGGAACTT TCTACGAACG GGACTATGAG 
TACGAGCGCC ACCATGAGCC GCCAGCCTAC GAACAGAGTT TATCACAGGG CAACCCCCAT 
CCACATTCAC ACCCTCATCC CCGGTCACCA AGGACTGCTC GTCACGGGCC TCAACCACAC 
CCGCGGCGTA TGCCTAACGG CTACCGTTCC TCCTCGCCCT CCCCGCACCG GCGCGGCCCC 
CACCCAGGCC CACACCGGCC ACCGCATGGC CGTGGGCCCC GGAAGGGGTT GCACGAACAA 
TATAGCGAGA CGGACGAGGA TGATTGGTGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ab ( XM_021479651.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335326.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X11, mRNA.

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CloneID	ODa147903
Clone ID Related Accession (Same CDS sequence)	XM_021479651.1
Accession Version	XM_021479651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGGCGCAT TCATTATAAG 
GATATGTACA GTTTATTGCG GGTTATCGAC CCCCCGCTGG GCTTAGGCAA GAAATGTCCT 
CATAGGGTGG CCTGCAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCAGCGACA 
GACCTGACAG TGGGCAAGAT CTACGCCGCC ATGATGATCA TGGAGTACTA CCGGCAGAGC 
AAGGCCAAAA AGCTGCAGGC GCTTCGTGAG GAGCAGCGCA TGGAGCCGTC CCCAAACCAG 
GAAGGGGGAC CGGGGCTGAA TGGCCTGCCC GAGCTGCCGC AGGAGCCCGT TAACAGCCTG 
CCTCCAGAGG TTGGAATGAA TGAAAGCCAG TCATGGGTGA CGTCACGCGC CCAGGAGATG 
TGCCAGAAGT CCGTTCCAGA AAACTGGAAC CCTGACAGAC CATATCCTGA TGACCATCAT 
GACAGCCGCC ACAATCCACA GACGGTAGAA ATGAGAGAGT TTGGGCCTGT TGGGGACGGT 
TACTCGGACA CTGAGCCCTA TCACCCAATG GAAGGGCATG GCCGGACCAT CTCCATGCCT 
CGCCTCTCTG CAGACAACCA ACGACGGAGA CACAGGCCGC GAGGAAGTAA TCTCAGTACC 
ATCACCGACA CAAGTCCAAT GCGTCGTTCT ACATCCTCAT TGGTCCATGG ACGTTCAGGT 
CGAGGCGTGC GATTGGATGA TTACTCTCTG GAACGGGTGG TGTCAGAAGA GGGTCACCGA 
CATGCTCCCC GAAGGAGAGA TCGTGATCGA GACCGGGATA GAGATCGCAG TCATCGAACC 
TCAGAGAGAT CCCTCGCTCG CTATACAGAT GCAGACACAG GTCTGGGTAC AGATCTTAGC 
ACCACTACAC AGTCGGGTGA CCTCCCACCT AAAGAAAGGG AACGAGAACG GGGTCGAGCA 
AAAGACCGTC GCCACCACCA CCACCATCAT CACCACCACG GCTCGGTGGA TAAAGATCGG 
TACAGTCACG AGCGCGACTA CCATCGTCAT CCTCAAGACA GATCAGATCG CCAATGGTCC 
CGCTCTCCGA GTGAGGGCAG AGGGCACAGA CAGGGCAGTA GTTCTGTGAG CGGAAGCCCC 
GTTCCCTCGA CGTCGGGGAC CAGCACCCCC CGGCGTGGCC GTCGCCAGTT GCCCCAGACC 
CCATCCACTC CACGCCCTCA CGTCACCTAC TCCCCTGCGG TCCGCAAGCC TCCATATGGC 
TCCCCAGGCC AGGGTCGACT CCGCTCCCCT TCCCCGCGAA ACTTTTCTCC GCCAGGTCAT 
GAACCGCCAT ACCACCGTCC ACCCTCCCGC CATGCCTCAC CTCATCCTGG CTCACCCCGG 
CATGCCTCTC CGCGTTCCCC CAGGCACACG TCGCCCAGGT CCCCTCGTCA CGGCCGCTGG 
GGCCCTCCGG ACAGCCTAGA AGGTGATGGA ACTTTCTACG AACGGGACTA TGAGTACGAG 
CGCCACCATG AGCCGCCAGC CTACGAACAG AGTTTATCAC AGGGCAACCC CCATCCACAT 
TCACACCCTC ATCCCCGGTC ACCAAGGACT GCTCGTCACG GGCCTCAACC ACACCCGCGG 
CGTATGCCTA ACGGCTACCG TTCCTCCTCG CCCTCCCCGC ACCGGCGCGG CCCCCACCCA 
GGCCCACACC GGCCACCGCA TGGCCGTGGG CCCCGGAAGG GGTTGCACGA ACAATATAGC 
GAGACGGACG AGGATGATTG GTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335326.1
CDS	1437..8423
Translation	MARFGDEVPARYGGGGGGGSGQGGPGRGGSRQGGPPGAQRMYKQSMAQRARTMAIYNPIPVRQNCLTVNRSLFLFSEDNIVRKYAKKITEWPPFEWMILTTIIANCIVLALEQHLPVGDKTPLSERLEETEPYFIAIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSSVGSELDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGVLLFVAILMFAIIGLEFYMGKFHTTCFNTITGEMYDERPCGARTCDANNTQCQGYWLGPNYGITQFDNVLFAVLTVFQCITMEGWTDMLYYSNDALGSAWNWMYYVPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILAEDDNDTGSRRRPTLKKNKADLLNPQEGVDHMGDSVGSPFARGSMSGKGDETPFSKKERRLRFLIRKMVKTQAFYWTVISLVALNTLCVAVVHYDQPELLSDFLYYAEFIFLGLFMSEMLIKMYGLGTRPYFHSSFNCFDCAVIVGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFELGTPPTNFDTFPAAIMTVFQILTGEDWNVVMYDGIKSQGGVDKGMIFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEATTQKMALQKAKEVAEVSPLSAANLSIAAKEQQKNSKGSKSVWEQRTSEIRRQNLMTSREALYNELDAEDHWKMNYRNRPDVKTHLDRPLVVNPQENRNNNTNKPRPDDPNPQERSEEHPSRSHHHHSHHHHHHHHHTHHSSRQSLHLPELAETGFECDVEGAGEHRRPRHHQHRRRDEDGGERGRKHRDHHSRGETAGGDAEDGQEKRQRRHRHANHGDGDGRRERSRLHRNKKEGSLSTARPIQPGLPHTESQYSEDLDNLRNSSKMAIHDPDPDLYDPYYDPEQANNLLNLRGPDLHDSSLILTDSSKLEHTAIDMPPIYPSLNAKLQVNKNANTEPAKEGEKKEEEEDDGGDEEGHKRIPPFSSCFILSTTNPFRRCCHYILTLRYFEMCILSVIAMSSIALAAEDPVSQDSERNKVLRYFDYVFTGVFTFEMLIKMVVLGLILHEGSYFRDLWNFLDFVVVSGALVAFAFTSFSGGSGKGKDISIIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFARDCRGEYLVYERDNEVKSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPDVLKHSVDSTYENRGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYSIMSLIALNTIILMMKYNGASDAYDKVLKNLNIVFTTFFFMESILKIIAFGVRNFFRDAWNIFDFVSVIGSITDILVTELVNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAILFFIYAIIGMQLFGNLALDDEGAINEHNNFRTFFMALMLLFRSATGEAWHDIMLSCLGGMPCEPEEVGSECGSNVAYAYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKKLQALREEQRMEPSPNQEGGPGLNGLPELPQEPVNSLPPEVGMNESQSWVTSRAQEMCQKSVPENWNPDRPYPDDHHDSRHNPQTVEMREFGPVGDGYSDTEPYHPMEGHGRTISMPRLSADNQRRRHRPRGSNLSTITDTSPMRRSTSSLVHGRSGRGVRLDDYSLERVVSEEGHRHAPRRRDRDRDRDRDRSHRTSERSLARYTDADTGLGTDLSTTTQSGDLPPKERERERGRAKDRRHHHHHHHHHGSVDKDRYSHERDYHRHPQDRSDRQWSRSPSEGRGHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPSTPRPHVTYSPAVRKPPYGSPGQGRLRSPSPRNFSPPGHEPPYHRPPSRHASPHPGSPRHASPRSPRHTSPRSPRHGRWGPPDSLEGDGTFYERDYEYERHHEPPAYEQSLSQGNPHPHSHPHPRSPRTARHGPQPHPRRMPNGYRSSSPSPHRRGPHPGPHRPPHGRGPRKGLHEQYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021479651.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479651.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGGCGCAT TCATTATAAG 
GATATGTACA GTTTATTGCG GGTTATCGAC CCCCCGCTGG GCTTAGGCAA GAAATGTCCT 
CATAGGGTGG CCTGCAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCAGCGACA 
GACCTGACAG TGGGCAAGAT CTACGCCGCC ATGATGATCA TGGAGTACTA CCGGCAGAGC 
AAGGCCAAAA AGCTGCAGGC GCTTCGTGAG GAGCAGCGCA TGGAGCCGTC CCCAAACCAG 
GAAGGGGGAC CGGGGCTGAA TGGCCTGCCC GAGCTGCCGC AGGAGCCCGT TAACAGCCTG 
CCTCCAGAGG TTGGAATGAA TGAAAGCCAG TCATGGGTGA CGTCACGCGC CCAGGAGATG 
TGCCAGAAGT CCGTTCCAGA AAACTGGAAC CCTGACAGAC CATATCCTGA TGACCATCAT 
GACAGCCGCC ACAATCCACA GACGGTAGAA ATGAGAGAGT TTGGGCCTGT TGGGGACGGT 
TACTCGGACA CTGAGCCCTA TCACCCAATG GAAGGGCATG GCCGGACCAT CTCCATGCCT 
CGCCTCTCTG CAGACAACCA ACGACGGAGA CACAGGCCGC GAGGAAGTAA TCTCAGTACC 
ATCACCGACA CAAGTCCAAT GCGTCGTTCT ACATCCTCAT TGGTCCATGG ACGTTCAGGT 
CGAGGCGTGC GATTGGATGA TTACTCTCTG GAACGGGTGG TGTCAGAAGA GGGTCACCGA 
CATGCTCCCC GAAGGAGAGA TCGTGATCGA GACCGGGATA GAGATCGCAG TCATCGAACC 
TCAGAGAGAT CCCTCGCTCG CTATACAGAT GCAGACACAG GTCTGGGTAC AGATCTTAGC 
ACCACTACAC AGTCGGGTGA CCTCCCACCT AAAGAAAGGG AACGAGAACG GGGTCGAGCA 
AAAGACCGTC GCCACCACCA CCACCATCAT CACCACCACG GCTCGGTGGA TAAAGATCGG 
TACAGTCACG AGCGCGACTA CCATCGTCAT CCTCAAGACA GATCAGATCG CCAATGGTCC 
CGCTCTCCGA GTGAGGGCAG AGGGCACAGA CAGGGCAGTA GTTCTGTGAG CGGAAGCCCC 
GTTCCCTCGA CGTCGGGGAC CAGCACCCCC CGGCGTGGCC GTCGCCAGTT GCCCCAGACC 
CCATCCACTC CACGCCCTCA CGTCACCTAC TCCCCTGCGG TCCGCAAGCC TCCATATGGC 
TCCCCAGGCC AGGGTCGACT CCGCTCCCCT TCCCCGCGAA ACTTTTCTCC GCCAGGTCAT 
GAACCGCCAT ACCACCGTCC ACCCTCCCGC CATGCCTCAC CTCATCCTGG CTCACCCCGG 
CATGCCTCTC CGCGTTCCCC CAGGCACACG TCGCCCAGGT CCCCTCGTCA CGGCCGCTGG 
GGCCCTCCGG ACAGCCTAGA AGGTGATGGA ACTTTCTACG AACGGGACTA TGAGTACGAG 
CGCCACCATG AGCCGCCAGC CTACGAACAG AGTTTATCAC AGGGCAACCC CCATCCACAT 
TCACACCCTC ATCCCCGGTC ACCAAGGACT GCTCGTCACG GGCCTCAACC ACACCCGCGG 
CGTATGCCTA ACGGCTACCG TTCCTCCTCG CCCTCCCCGC ACCGGCGCGG CCCCCACCCA 
GGCCCACACC GGCCACCGCA TGGCCGTGGG CCCCGGAAGG GGTTGCACGA ACAATATAGC 
GAGACGGACG AGGATGATTG GTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ab ( XM_021479652.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335327.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X12, mRNA.

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CloneID	ODa147904
Clone ID Related Accession (Same CDS sequence)	XM_021479652.1
Accession Version	XM_021479652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGGCGCAT TCATTATAAG 
GATATGTACA GTTTATTGCG GGTTATCGAC CCCCCGCTGG GCTTAGGCAA GAAATGTCCT 
CATAGGGTGG CCTGCAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCAGCGACA 
GACCTGACAG TGGGCAAGAT CTACGCCGCC ATGATGATCA TGGAGTACTA CCGGCAGAGC 
AAGGCCAAAA AGCTGCAGGC GCTTCGTGAG GAGCAGAACC GAACGCCATT AATGTTTCAG 
CGCATGGAGC CGTCCCCAAA CCAGGAAGGG GGACCGGGGC TGAATGGCCT GCCCGAGCTG 
CCGCAGGAGC CCGTTAACAG CCTGCCTCCA GAGGTTGGAA TGAATGAAAG CCAGTCATGG 
GTGACGTCAC GCGCCCAGGA GATGTGCCAG AAGTCCGTTC CAGAAAACTG GAACCCTGAC 
AGACCATATC CTGATGACCA TCATGACAGC CGCCACAATC CACAGACGGT AGAAATGAGA 
GAGTTTGGGC CTGTTGGGGA CGGTTACTCG GACACTGAGC CCTATCACCC AATGGAAGGG 
CATGGCCGGA CCATCTCCAT GCCTCGCCTC TCTGCAGACA ACCAAACCAT CACCGACACA 
AGTCCAATGC GTCGTTCTAC ATCCTCATTG GTCCATGGAC GTTCAGGTCG AGGCGTGCGA 
TTGGATGATT ACTCTCTGGA ACGGGTGGTG TCAGAAGAGG GTCACCGACA TGCTCCCCGA 
AGGAGAGATC GTGATCGAGA CCGGGATAGA GATCGCAGTC ATCGAACCTC AGAGAGATCC 
CTCGCTCGCT ATACAGATGC AGACACAGGT CTGGGTACAG ATCTTAGCAC CACTACACAG 
TCGGGTGACC TCCCACCTAA AGAAAGGGAA CGAGAACGGG GTCGAGCAAA AGACCGTCGC 
CACCACCACC ACCATCATCA CCACCACGGC TCGGTGGATA AAGATCGGTA CAGTCACGAG 
CGCGACTACC ATCGTCATCC TCAAGACAGA TCAGATCGCC AATGGTCCCG CTCTCCGAGT 
GAGGGCAGAG GGCACAGACA GGGCAGTAGT TCTGTGAGCG GAAGCCCCGT TCCCTCGACG 
TCGGGGACCA GCACCCCCCG GCGTGGCCGT CGCCAGTTGC CCCAGACCCC ATCCACTCCA 
CGCCCTCACG TCACCTACTC CCCTGCGGTC CGCAAGCCTC CATATGGCTC CCCAGGCCAG 
GGTCGACTCC GCTCCCCTTC CCCGCGAAAC TTTTCTCCGC CAGGTCATGA ACCGCCATAC 
CACCGTCCAC CCTCCCGCCA TGCCTCACCT CATCCTGGCT CACCCCGGCA TGCCTCTCCG 
CGTTCCCCCA GGCACACGTC GCCCAGGTCC CCTCGTCACG GCCGCTGGGG CCCTCCGGAC 
AGCCTAGAAG GTGATGGAAC TTTCTACGAA CGGGACTATG AGTACGAGCG CCACCATGAG 
CCGCCAGCCT ACGAACAGAG TTTATCACAG GGCAACCCCC ATCCACATTC ACACCCTCAT 
CCCCGGTCAC CAAGGACTGC TCGTCACGGG CCTCAACCAC ACCCGCGGCG TATGCCTAAC 
GGCTACCGTT CCTCCTCGCC CTCCCCGCAC CGGCGCGGCC CCCACCCAGG CCCACACCGG 
CCACCGCATG GCCGTGGGCC CCGGAAGGGG TTGCACGAAC AATATAGCGA GACGGACGAG 
GATGATTGGT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335327.1
CDS	1436..8410
Translation	MARFGDEVPARYGGGGGGGSGQGGPGRGGSRQGGPPGAQRMYKQSMAQRARTMAIYNPIPVRQNCLTVNRSLFLFSEDNIVRKYAKKITEWPPFEWMILTTIIANCIVLALEQHLPVGDKTPLSERLEETEPYFIAIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSSVGSELDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGVLLFVAILMFAIIGLEFYMGKFHTTCFNTITGEMYDERPCGARTCDANNTQCQGYWLGPNYGITQFDNVLFAVLTVFQCITMEGWTDMLYYSNDALGSAWNWMYYVPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILAEDDNDTGSRRRPTLKKNKADLLNPQEGVDHMGDSVGSPFARGSMSGKGDETPFSKKERRLRFLIRKMVKTQAFYWTVISLVALNTLCVAVVHYDQPELLSDFLYYAEFIFLGLFMSEMLIKMYGLGTRPYFHSSFNCFDCAVIVGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFELGTPPTNFDTFPAAIMTVFQILTGEDWNVVMYDGIKSQGGVDKGMIFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEATTQKMALQKAKEVAEVSPLSAANLSIAAKEQQKNSKGSKSVWEQRTSEIRRQNLMTSREALYNELDAEDHWKMNYRNRPDVKTHLDRPLVVNPQENRNNNTNKPRPDDPNPQERSEEHPSRSHHHHSHHHHHHHHHTHHSSRQSLHLPELAETGFECDVEGAGEHRRPRHHQHRRRDEDGGERGRKHRDHHSRGETAGGDAEDGQEKRQRRHRHANHGDGDGRRERSRLHRNKKEGSLSTARPIQPGLPHTESQYSEDLDNLRNSSKMAIHDPDPDLYDPYYDPEQANNLLNLRGPDLHDSSLILTDSSKLEHTAIDMPPIYPSLNAKLQVNKNANTEPAKEGEKKEEEEDDGGDEEGHKRIPPFSSCFILSTTNPFRRCCHYILTLRYFEMCILSVIAMSSIALAAEDPVSQDSERNKVLRYFDYVFTGVFTFEMLIKMVVLGLILHEGSYFRDLWNFLDFVVVSGALVAFAFTSFSGGSGKGKDISIIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFARDCRGEYLVYERDNEVKSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPDVLKHSVDSTYENRGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYSIMSLIALNTIILMMKYNGASDAYDKVLKNLNIVFTTFFFMESILKIIAFGVRNFFRDAWNIFDFVSVIGSITDILVTELVNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAILFFIYAIIGMQLFGNLALDDEGAINEHNNFRTFFMALMLLFRSATGEAWHDIMLSCLGGMPCEPEEVGSECGSNVAYAYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKKLQALREEQNRTPLMFQRMEPSPNQEGGPGLNGLPELPQEPVNSLPPEVGMNESQSWVTSRAQEMCQKSVPENWNPDRPYPDDHHDSRHNPQTVEMREFGPVGDGYSDTEPYHPMEGHGRTISMPRLSADNQTITDTSPMRRSTSSLVHGRSGRGVRLDDYSLERVVSEEGHRHAPRRRDRDRDRDRDRSHRTSERSLARYTDADTGLGTDLSTTTQSGDLPPKERERERGRAKDRRHHHHHHHHHGSVDKDRYSHERDYHRHPQDRSDRQWSRSPSEGRGHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPSTPRPHVTYSPAVRKPPYGSPGQGRLRSPSPRNFSPPGHEPPYHRPPSRHASPHPGSPRHASPRSPRHTSPRSPRHGRWGPPDSLEGDGTFYERDYEYERHHEPPAYEQSLSQGNPHPHSHPHPRSPRTARHGPQPHPRRMPNGYRSSSPSPHRRGPHPGPHRPPHGRGPRKGLHEQYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021479652.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479652.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGGCGCAT TCATTATAAG 
GATATGTACA GTTTATTGCG GGTTATCGAC CCCCCGCTGG GCTTAGGCAA GAAATGTCCT 
CATAGGGTGG CCTGCAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCAGCGACA 
GACCTGACAG TGGGCAAGAT CTACGCCGCC ATGATGATCA TGGAGTACTA CCGGCAGAGC 
AAGGCCAAAA AGCTGCAGGC GCTTCGTGAG GAGCAGAACC GAACGCCATT AATGTTTCAG 
CGCATGGAGC CGTCCCCAAA CCAGGAAGGG GGACCGGGGC TGAATGGCCT GCCCGAGCTG 
CCGCAGGAGC CCGTTAACAG CCTGCCTCCA GAGGTTGGAA TGAATGAAAG CCAGTCATGG 
GTGACGTCAC GCGCCCAGGA GATGTGCCAG AAGTCCGTTC CAGAAAACTG GAACCCTGAC 
AGACCATATC CTGATGACCA TCATGACAGC CGCCACAATC CACAGACGGT AGAAATGAGA 
GAGTTTGGGC CTGTTGGGGA CGGTTACTCG GACACTGAGC CCTATCACCC AATGGAAGGG 
CATGGCCGGA CCATCTCCAT GCCTCGCCTC TCTGCAGACA ACCAAACCAT CACCGACACA 
AGTCCAATGC GTCGTTCTAC ATCCTCATTG GTCCATGGAC GTTCAGGTCG AGGCGTGCGA 
TTGGATGATT ACTCTCTGGA ACGGGTGGTG TCAGAAGAGG GTCACCGACA TGCTCCCCGA 
AGGAGAGATC GTGATCGAGA CCGGGATAGA GATCGCAGTC ATCGAACCTC AGAGAGATCC 
CTCGCTCGCT ATACAGATGC AGACACAGGT CTGGGTACAG ATCTTAGCAC CACTACACAG 
TCGGGTGACC TCCCACCTAA AGAAAGGGAA CGAGAACGGG GTCGAGCAAA AGACCGTCGC 
CACCACCACC ACCATCATCA CCACCACGGC TCGGTGGATA AAGATCGGTA CAGTCACGAG 
CGCGACTACC ATCGTCATCC TCAAGACAGA TCAGATCGCC AATGGTCCCG CTCTCCGAGT 
GAGGGCAGAG GGCACAGACA GGGCAGTAGT TCTGTGAGCG GAAGCCCCGT TCCCTCGACG 
TCGGGGACCA GCACCCCCCG GCGTGGCCGT CGCCAGTTGC CCCAGACCCC ATCCACTCCA 
CGCCCTCACG TCACCTACTC CCCTGCGGTC CGCAAGCCTC CATATGGCTC CCCAGGCCAG 
GGTCGACTCC GCTCCCCTTC CCCGCGAAAC TTTTCTCCGC CAGGTCATGA ACCGCCATAC 
CACCGTCCAC CCTCCCGCCA TGCCTCACCT CATCCTGGCT CACCCCGGCA TGCCTCTCCG 
CGTTCCCCCA GGCACACGTC GCCCAGGTCC CCTCGTCACG GCCGCTGGGG CCCTCCGGAC 
AGCCTAGAAG GTGATGGAAC TTTCTACGAA CGGGACTATG AGTACGAGCG CCACCATGAG 
CCGCCAGCCT ACGAACAGAG TTTATCACAG GGCAACCCCC ATCCACATTC ACACCCTCAT 
CCCCGGTCAC CAAGGACTGC TCGTCACGGG CCTCAACCAC ACCCGCGGCG TATGCCTAAC 
GGCTACCGTT CCTCCTCGCC CTCCCCGCAC CGGCGCGGCC CCCACCCAGG CCCACACCGG 
CCACCGCATG GCCGTGGGCC CCGGAAGGGG TTGCACGAAC AATATAGCGA GACGGACGAG 
GATGATTGGT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ab ( XM_021479653.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335328.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X13, mRNA.

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CloneID	ODa147905
Clone ID Related Accession (Same CDS sequence)	XM_021479653.1
Accession Version	XM_021479653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGGCGCAT TCATTATAAG 
GATATGTACA GTTTATTGCG GGTTATCGAC CCCCCGCTGG GCTTAGGCAA GAAATGTCCT 
CATAGGGTGG CCTGCAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCAGCGACA 
GACCTGACAG TGGGCAAGAT CTACGCCGCC ATGATGATCA TGGAGTACTA CCGGCAGAGC 
AAGGCCAAAA AGCTGCAGGC GCTTCGTGAG GAGCAGAACC GAACGCCATT AATGTTTCAG 
CGCATGGAGC CGTCCCCAAA CCAGGAAGGG GGACCGGGGC TGAATGGCCT GCCCGAGCTG 
CCGCAGGAGC CCGTTAACAG CCTGCCTCCA GAGGTTGGAA TGAATGAAAG CCAGTCATGG 
GTGACGTCAC GCGCCCAGGA GATGTGCCAG AAGTCCGTTC CAGAAAACTG GAACCCTGAC 
AGACCATATC CTGATGACCA TCATGACAGC CGCCACAATC CACAGCGACG GAGACACAGG 
CCGCGAGGAA GTAATCTCAG TACCATCACC GACACAAGTC CAATGCGTCG TTCTACATCC 
TCATTGGTCC ATGGACGTTC AGGTCGAGGC GTGCGATTGG ATGATTACTC TCTGGAACGG 
GTGGTGTCAG AAGAGGGTCA CCGACATGCT CCCCGAAGGA GAGATCGTGA TCGAGACCGG 
GATAGAGATC GCAGTCATCG AACCTCAGAG AGATCCCTCG CTCGCTATAC AGATGCAGAC 
ACAGGTCTGG GTACAGATCT TAGCACCACT ACACAGTCGG GTGACCTCCC ACCTAAAGAA 
AGGGAACGAG AACGGGGTCG AGCAAAAGAC CGTCGCCACC ACCACCACCA TCATCACCAC 
CACGGCTCGG TGGATAAAGA TCGGTACAGT CACGAGCGCG ACTACCATCG TCATCCTCAA 
GACAGATCAG ATCGCCAATG GTCCCGCTCT CCGAGTGAGG GCAGAGGGCA CAGACAGGGC 
AGTAGTTCTG TGAGCGGAAG CCCCGTTCCC TCGACGTCGG GGACCAGCAC CCCCCGGCGT 
GGCCGTCGCC AGTTGCCCCA GACCCCATCC ACTCCACGCC CTCACGTCAC CTACTCCCCT 
GCGGTCCGCA AGCCTCCATA TGGCTCCCCA GGCCAGGGTC GACTCCGCTC CCCTTCCCCG 
CGAAACTTTT CTCCGCCAGG TCATGAACCG CCATACCACC GTCCACCCTC CCGCCATGCC 
TCACCTCATC CTGGCTCACC CCGGCATGCC TCTCCGCGTT CCCCCAGGCA CACGTCGCCC 
AGGTCCCCTC GTCACGGCCG CTGGGGCCCT CCGGACAGCC TAGAAGGTGA TGGAACTTTC 
TACGAACGGG ACTATGAGTA CGAGCGCCAC CATGAGCCGC CAGCCTACGA ACAGAGTTTA 
TCACAGGGCA ACCCCCATCC ACATTCACAC CCTCATCCCC GGTCACCAAG GACTGCTCGT 
CACGGGCCTC AACCACACCC GCGGCGTATG CCTAACGGCT ACCGTTCCTC CTCGCCCTCC 
CCGCACCGGC GCGGCCCCCA CCCAGGCCCA CACCGGCCAC CGCATGGCCG TGGGCCCCGG 
AAGGGGTTGC ACGAACAATA TAGCGAGACG GACGAGGATG ATTGGTGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335328.1
CDS	1436..8326
Translation	MARFGDEVPARYGGGGGGGSGQGGPGRGGSRQGGPPGAQRMYKQSMAQRARTMAIYNPIPVRQNCLTVNRSLFLFSEDNIVRKYAKKITEWPPFEWMILTTIIANCIVLALEQHLPVGDKTPLSERLEETEPYFIAIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSSVGSELDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGVLLFVAILMFAIIGLEFYMGKFHTTCFNTITGEMYDERPCGARTCDANNTQCQGYWLGPNYGITQFDNVLFAVLTVFQCITMEGWTDMLYYSNDALGSAWNWMYYVPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILAEDDNDTGSRRRPTLKKNKADLLNPQEGVDHMGDSVGSPFARGSMSGKGDETPFSKKERRLRFLIRKMVKTQAFYWTVISLVALNTLCVAVVHYDQPELLSDFLYYAEFIFLGLFMSEMLIKMYGLGTRPYFHSSFNCFDCAVIVGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFELGTPPTNFDTFPAAIMTVFQILTGEDWNVVMYDGIKSQGGVDKGMIFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEATTQKMALQKAKEVAEVSPLSAANLSIAAKEQQKNSKGSKSVWEQRTSEIRRQNLMTSREALYNELDAEDHWKMNYRNRPDVKTHLDRPLVVNPQENRNNNTNKPRPDDPNPQERSEEHPSRSHHHHSHHHHHHHHHTHHSSRQSLHLPELAETGFECDVEGAGEHRRPRHHQHRRRDEDGGERGRKHRDHHSRGETAGGDAEDGQEKRQRRHRHANHGDGDGRRERSRLHRNKKEGSLSTARPIQPGLPHTESQYSEDLDNLRNSSKMAIHDPDPDLYDPYYDPEQANNLLNLRGPDLHDSSLILTDSSKLEHTAIDMPPIYPSLNAKLQVNKNANTEPAKEGEKKEEEEDDGGDEEGHKRIPPFSSCFILSTTNPFRRCCHYILTLRYFEMCILSVIAMSSIALAAEDPVSQDSERNKVLRYFDYVFTGVFTFEMLIKMVVLGLILHEGSYFRDLWNFLDFVVVSGALVAFAFTSFSGGSGKGKDISIIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFARDCRGEYLVYERDNEVKSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPDVLKHSVDSTYENRGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYSIMSLIALNTIILMMKYNGASDAYDKVLKNLNIVFTTFFFMESILKIIAFGVRNFFRDAWNIFDFVSVIGSITDILVTELVNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAILFFIYAIIGMQLFGNLALDDEGAINEHNNFRTFFMALMLLFRSATGEAWHDIMLSCLGGMPCEPEEVGSECGSNVAYAYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKKLQALREEQNRTPLMFQRMEPSPNQEGGPGLNGLPELPQEPVNSLPPEVGMNESQSWVTSRAQEMCQKSVPENWNPDRPYPDDHHDSRHNPQRRRHRPRGSNLSTITDTSPMRRSTSSLVHGRSGRGVRLDDYSLERVVSEEGHRHAPRRRDRDRDRDRDRSHRTSERSLARYTDADTGLGTDLSTTTQSGDLPPKERERERGRAKDRRHHHHHHHHHGSVDKDRYSHERDYHRHPQDRSDRQWSRSPSEGRGHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPSTPRPHVTYSPAVRKPPYGSPGQGRLRSPSPRNFSPPGHEPPYHRPPSRHASPHPGSPRHASPRSPRHTSPRSPRHGRWGPPDSLEGDGTFYERDYEYERHHEPPAYEQSLSQGNPHPHSHPHPRSPRTARHGPQPHPRRMPNGYRSSSPSPHRRGPHPGPHRPPHGRGPRKGLHEQYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021479653.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479653.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGGCGCAT TCATTATAAG 
GATATGTACA GTTTATTGCG GGTTATCGAC CCCCCGCTGG GCTTAGGCAA GAAATGTCCT 
CATAGGGTGG CCTGCAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCAGCGACA 
GACCTGACAG TGGGCAAGAT CTACGCCGCC ATGATGATCA TGGAGTACTA CCGGCAGAGC 
AAGGCCAAAA AGCTGCAGGC GCTTCGTGAG GAGCAGAACC GAACGCCATT AATGTTTCAG 
CGCATGGAGC CGTCCCCAAA CCAGGAAGGG GGACCGGGGC TGAATGGCCT GCCCGAGCTG 
CCGCAGGAGC CCGTTAACAG CCTGCCTCCA GAGGTTGGAA TGAATGAAAG CCAGTCATGG 
GTGACGTCAC GCGCCCAGGA GATGTGCCAG AAGTCCGTTC CAGAAAACTG GAACCCTGAC 
AGACCATATC CTGATGACCA TCATGACAGC CGCCACAATC CACAGCGACG GAGACACAGG 
CCGCGAGGAA GTAATCTCAG TACCATCACC GACACAAGTC CAATGCGTCG TTCTACATCC 
TCATTGGTCC ATGGACGTTC AGGTCGAGGC GTGCGATTGG ATGATTACTC TCTGGAACGG 
GTGGTGTCAG AAGAGGGTCA CCGACATGCT CCCCGAAGGA GAGATCGTGA TCGAGACCGG 
GATAGAGATC GCAGTCATCG AACCTCAGAG AGATCCCTCG CTCGCTATAC AGATGCAGAC 
ACAGGTCTGG GTACAGATCT TAGCACCACT ACACAGTCGG GTGACCTCCC ACCTAAAGAA 
AGGGAACGAG AACGGGGTCG AGCAAAAGAC CGTCGCCACC ACCACCACCA TCATCACCAC 
CACGGCTCGG TGGATAAAGA TCGGTACAGT CACGAGCGCG ACTACCATCG TCATCCTCAA 
GACAGATCAG ATCGCCAATG GTCCCGCTCT CCGAGTGAGG GCAGAGGGCA CAGACAGGGC 
AGTAGTTCTG TGAGCGGAAG CCCCGTTCCC TCGACGTCGG GGACCAGCAC CCCCCGGCGT 
GGCCGTCGCC AGTTGCCCCA GACCCCATCC ACTCCACGCC CTCACGTCAC CTACTCCCCT 
GCGGTCCGCA AGCCTCCATA TGGCTCCCCA GGCCAGGGTC GACTCCGCTC CCCTTCCCCG 
CGAAACTTTT CTCCGCCAGG TCATGAACCG CCATACCACC GTCCACCCTC CCGCCATGCC 
TCACCTCATC CTGGCTCACC CCGGCATGCC TCTCCGCGTT CCCCCAGGCA CACGTCGCCC 
AGGTCCCCTC GTCACGGCCG CTGGGGCCCT CCGGACAGCC TAGAAGGTGA TGGAACTTTC 
TACGAACGGG ACTATGAGTA CGAGCGCCAC CATGAGCCGC CAGCCTACGA ACAGAGTTTA 
TCACAGGGCA ACCCCCATCC ACATTCACAC CCTCATCCCC GGTCACCAAG GACTGCTCGT 
CACGGGCCTC AACCACACCC GCGGCGTATG CCTAACGGCT ACCGTTCCTC CTCGCCCTCC 
CCGCACCGGC GCGGCCCCCA CCCAGGCCCA CACCGGCCAC CGCATGGCCG TGGGCCCCGG 
AAGGGGTTGC ACGAACAATA TAGCGAGACG GACGAGGATG ATTGGTGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ab ( XM_021479654.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335329.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X14, mRNA.

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CloneID	ODa147906
Clone ID Related Accession (Same CDS sequence)	XM_021479654.1
Accession Version	XM_021479654.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGGCGCAT TCATTATAAG 
GATATGTACA GTTTATTGCG GGTTATCGAC CCCCCGCTGG GCTTAGGCAA GAAATGTCCT 
CATAGGGTGG CCTGCAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCAGCGACA 
GACCTGACAG TGGGCAAGAT CTACGCCGCC ATGATGATCA TGGAGTACTA CCGGCAGAGC 
AAGGCCAAAA AGCTGCAGGC GCTTCGTGAG GAGCAGAACC GAACGCCATT AATGTTTCAG 
CGCATGGAGC CGTCCCCAAA CCAGGAAGGG GGACCGGGGC TGAATGGCCT GCCCGAGCTG 
CCGCAGGAGC CCGTTAACAG CCTGCCTCCA GAGGTTGGAA TGAATGAAAG CCAGTCATGG 
GTGACGTCAC GCGCCCAGGA GATGTGCCAG AAGTCCGTTC CAGAAAACTG GAACCCTGAC 
AGACCATATC CTGATGACCA TCATGACAGC CGCCACAATC CACAGACCAT CACCGACACA 
AGTCCAATGC GTCGTTCTAC ATCCTCATTG GTCCATGGAC GTTCAGGTCG AGGCGTGCGA 
TTGGATGATT ACTCTCTGGA ACGGGTGGTG TCAGAAGAGG GTCACCGACA TGCTCCCCGA 
AGGAGAGATC GTGATCGAGA CCGGGATAGA GATCGCAGTC ATCGAACCTC AGAGAGATCC 
CTCGCTCGCT ATACAGATGC AGACACAGGT CTGGGTACAG ATCTTAGCAC CACTACACAG 
TCGGGTGACC TCCCACCTAA AGAAAGGGAA CGAGAACGGG GTCGAGCAAA AGACCGTCGC 
CACCACCACC ACCATCATCA CCACCACGGC TCGGTGGATA AAGATCGGTA CAGTCACGAG 
CGCGACTACC ATCGTCATCC TCAAGACAGA TCAGATCGCC AATGGTCCCG CTCTCCGAGT 
GAGGGCAGAG GGCACAGACA GGGCAGTAGT TCTGTGAGCG GAAGCCCCGT TCCCTCGACG 
TCGGGGACCA GCACCCCCCG GCGTGGCCGT CGCCAGTTGC CCCAGACCCC ATCCACTCCA 
CGCCCTCACG TCACCTACTC CCCTGCGGTC CGCAAGCCTC CATATGGCTC CCCAGGCCAG 
GGTCGACTCC GCTCCCCTTC CCCGCGAAAC TTTTCTCCGC CAGGTCATGA ACCGCCATAC 
CACCGTCCAC CCTCCCGCCA TGCCTCACCT CATCCTGGCT CACCCCGGCA TGCCTCTCCG 
CGTTCCCCCA GGCACACGTC GCCCAGGTCC CCTCGTCACG GCCGCTGGGG CCCTCCGGAC 
AGCCTAGAAG GTGATGGAAC TTTCTACGAA CGGGACTATG AGTACGAGCG CCACCATGAG 
CCGCCAGCCT ACGAACAGAG TTTATCACAG GGCAACCCCC ATCCACATTC ACACCCTCAT 
CCCCGGTCAC CAAGGACTGC TCGTCACGGG CCTCAACCAC ACCCGCGGCG TATGCCTAAC 
GGCTACCGTT CCTCCTCGCC CTCCCCGCAC CGGCGCGGCC CCCACCCAGG CCCACACCGG 
CCACCGCATG GCCGTGGGCC CCGGAAGGGG TTGCACGAAC AATATAGCGA GACGGACGAG 
GATGATTGGT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335329.1
CDS	1435..8289
Translation	MARFGDEVPARYGGGGGGGSGQGGPGRGGSRQGGPPGAQRMYKQSMAQRARTMAIYNPIPVRQNCLTVNRSLFLFSEDNIVRKYAKKITEWPPFEWMILTTIIANCIVLALEQHLPVGDKTPLSERLEETEPYFIAIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSSVGSELDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGVLLFVAILMFAIIGLEFYMGKFHTTCFNTITGEMYDERPCGARTCDANNTQCQGYWLGPNYGITQFDNVLFAVLTVFQCITMEGWTDMLYYSNDALGSAWNWMYYVPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILAEDDNDTGSRRRPTLKKNKADLLNPQEGVDHMGDSVGSPFARGSMSGKGDETPFSKKERRLRFLIRKMVKTQAFYWTVISLVALNTLCVAVVHYDQPELLSDFLYYAEFIFLGLFMSEMLIKMYGLGTRPYFHSSFNCFDCAVIVGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFELGTPPTNFDTFPAAIMTVFQILTGEDWNVVMYDGIKSQGGVDKGMIFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEATTQKMALQKAKEVAEVSPLSAANLSIAAKEQQKNSKGSKSVWEQRTSEIRRQNLMTSREALYNELDAEDHWKMNYRNRPDVKTHLDRPLVVNPQENRNNNTNKPRPDDPNPQERSEEHPSRSHHHHSHHHHHHHHHTHHSSRQSLHLPELAETGFECDVEGAGEHRRPRHHQHRRRDEDGGERGRKHRDHHSRGETAGGDAEDGQEKRQRRHRHANHGDGDGRRERSRLHRNKKEGSLSTARPIQPGLPHTESQYSEDLDNLRNSSKMAIHDPDPDLYDPYYDPEQANNLLNLRGPDLHDSSLILTDSSKLEHTAIDMPPIYPSLNAKLQVNKNANTEPAKEGEKKEEEEDDGGDEEGHKRIPPFSSCFILSTTNPFRRCCHYILTLRYFEMCILSVIAMSSIALAAEDPVSQDSERNKVLRYFDYVFTGVFTFEMLIKMVVLGLILHEGSYFRDLWNFLDFVVVSGALVAFAFTSFSGGSGKGKDISIIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFARDCRGEYLVYERDNEVKSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPDVLKHSVDSTYENRGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYSIMSLIALNTIILMMKYNGASDAYDKVLKNLNIVFTTFFFMESILKIIAFGVRNFFRDAWNIFDFVSVIGSITDILVTELVNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAILFFIYAIIGMQLFGNLALDDEGAINEHNNFRTFFMALMLLFRSATGEAWHDIMLSCLGGMPCEPEEVGSECGSNVAYAYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKKLQALREEQNRTPLMFQRMEPSPNQEGGPGLNGLPELPQEPVNSLPPEVGMNESQSWVTSRAQEMCQKSVPENWNPDRPYPDDHHDSRHNPQTITDTSPMRRSTSSLVHGRSGRGVRLDDYSLERVVSEEGHRHAPRRRDRDRDRDRDRSHRTSERSLARYTDADTGLGTDLSTTTQSGDLPPKERERERGRAKDRRHHHHHHHHHGSVDKDRYSHERDYHRHPQDRSDRQWSRSPSEGRGHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPSTPRPHVTYSPAVRKPPYGSPGQGRLRSPSPRNFSPPGHEPPYHRPPSRHASPHPGSPRHASPRSPRHTSPRSPRHGRWGPPDSLEGDGTFYERDYEYERHHEPPAYEQSLSQGNPHPHSHPHPRSPRTARHGPQPHPRRMPNGYRSSSPSPHRRGPHPGPHRPPHGRGPRKGLHEQYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021479654.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479654.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGGCGCAT TCATTATAAG 
GATATGTACA GTTTATTGCG GGTTATCGAC CCCCCGCTGG GCTTAGGCAA GAAATGTCCT 
CATAGGGTGG CCTGCAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCAGCGACA 
GACCTGACAG TGGGCAAGAT CTACGCCGCC ATGATGATCA TGGAGTACTA CCGGCAGAGC 
AAGGCCAAAA AGCTGCAGGC GCTTCGTGAG GAGCAGAACC GAACGCCATT AATGTTTCAG 
CGCATGGAGC CGTCCCCAAA CCAGGAAGGG GGACCGGGGC TGAATGGCCT GCCCGAGCTG 
CCGCAGGAGC CCGTTAACAG CCTGCCTCCA GAGGTTGGAA TGAATGAAAG CCAGTCATGG 
GTGACGTCAC GCGCCCAGGA GATGTGCCAG AAGTCCGTTC CAGAAAACTG GAACCCTGAC 
AGACCATATC CTGATGACCA TCATGACAGC CGCCACAATC CACAGACCAT CACCGACACA 
AGTCCAATGC GTCGTTCTAC ATCCTCATTG GTCCATGGAC GTTCAGGTCG AGGCGTGCGA 
TTGGATGATT ACTCTCTGGA ACGGGTGGTG TCAGAAGAGG GTCACCGACA TGCTCCCCGA 
AGGAGAGATC GTGATCGAGA CCGGGATAGA GATCGCAGTC ATCGAACCTC AGAGAGATCC 
CTCGCTCGCT ATACAGATGC AGACACAGGT CTGGGTACAG ATCTTAGCAC CACTACACAG 
TCGGGTGACC TCCCACCTAA AGAAAGGGAA CGAGAACGGG GTCGAGCAAA AGACCGTCGC 
CACCACCACC ACCATCATCA CCACCACGGC TCGGTGGATA AAGATCGGTA CAGTCACGAG 
CGCGACTACC ATCGTCATCC TCAAGACAGA TCAGATCGCC AATGGTCCCG CTCTCCGAGT 
GAGGGCAGAG GGCACAGACA GGGCAGTAGT TCTGTGAGCG GAAGCCCCGT TCCCTCGACG 
TCGGGGACCA GCACCCCCCG GCGTGGCCGT CGCCAGTTGC CCCAGACCCC ATCCACTCCA 
CGCCCTCACG TCACCTACTC CCCTGCGGTC CGCAAGCCTC CATATGGCTC CCCAGGCCAG 
GGTCGACTCC GCTCCCCTTC CCCGCGAAAC TTTTCTCCGC CAGGTCATGA ACCGCCATAC 
CACCGTCCAC CCTCCCGCCA TGCCTCACCT CATCCTGGCT CACCCCGGCA TGCCTCTCCG 
CGTTCCCCCA GGCACACGTC GCCCAGGTCC CCTCGTCACG GCCGCTGGGG CCCTCCGGAC 
AGCCTAGAAG GTGATGGAAC TTTCTACGAA CGGGACTATG AGTACGAGCG CCACCATGAG 
CCGCCAGCCT ACGAACAGAG TTTATCACAG GGCAACCCCC ATCCACATTC ACACCCTCAT 
CCCCGGTCAC CAAGGACTGC TCGTCACGGG CCTCAACCAC ACCCGCGGCG TATGCCTAAC 
GGCTACCGTT CCTCCTCGCC CTCCCCGCAC CGGCGCGGCC CCCACCCAGG CCCACACCGG 
CCACCGCATG GCCGTGGGCC CCGGAAGGGG TTGCACGAAC AATATAGCGA GACGGACGAG 
GATGATTGGT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ab ( XM_021479655.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335330.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X15, mRNA.

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CloneID	ODa147907
Clone ID Related Accession (Same CDS sequence)	XM_021479655.1
Accession Version	XM_021479655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGGCGCAT TCATTATAAG 
GATATGTACA GTTTATTGCG GGTTATCGAC CCCCCGCTGG GCTTAGGCAA GAAATGTCCT 
CATAGGGTGG CCTGCAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCAGCGACA 
GACCTGACAG TGGGCAAGAT CTACGCCGCC ATGATGATCA TGGAGTACTA CCGGCAGAGC 
AAGGCCAAAA AGCTGCAGGC GCTTCGTGAG GAGCAGAACC GAACGCCATT AATGTTTCAG 
CGCATGGAGC CGTCCCCAAA CCAGGAAGGG GGACCGGGGC TGAATGGCCT GCCCGAGCTG 
CCGCAGGAGC CCGTTAACAG CCTGCCTCCA GAGGTTGGAA TGAATGAAAG CCAGTCATGG 
GTGACGTCAC GCGCCCAGGA GATGTGCCAG AAGTCCGTTC CAGAAAACTG GAACCCTGAC 
AGACCATATC CTGATGACCA TCATGACAGC CGCCACAATC CACAGACGGT AGAAATGAGA 
GAGTTTGGGC CTGTTGGGGA CGGTTACTCG GACACTGAGC CCTATCACCC AATGGAAGGG 
CATGGCCGGA CCATCTCCAT GCCTCGCCTC TCTGCAGACA ACCAACGACG GAGACACAGG 
CCGCGAGGAA GTAATCTCAG TACCATCACC GACACAAGTC CAATGCGTCG TTCTACATCC 
TCATTGGTCC ATGGACGTTC AGGTCGAGGC GTGCGATTGG ATGATTACTC TCTGGAACGG 
GTGGTGTCAG AAGAGGGTCA CCGACATGCT CCCCGAAGGA GAGATCGTGA TCGAGACCGG 
GATAGAGATC GCAGTCATCG AACCTCAGAG AGATCCCTCG CTCGCTATAC AGATGCAGAC 
ACAGGTCTGG GTACAGATCT TAGCACCACT ACACAGTCGG GTGACCTCCC ACCTAAAGAA 
AGGGAACGAG AACGGGGTCG AGCAAAAGAC CGTCGCCACC ACCACCACCA TCATCACCAC 
CACGGCTCGG TGGATAAAGA TCGGTACAGT CACGAGCGCG ACTACCATCG TCATCCTCAA 
GACAGATCAG ATCGCCAATG GTCCCGCTCT CCGAGTGAGG GCAGAGGGCA CAGACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335330.1
CDS	1441..7800
Translation	MARFGDEVPARYGGGGGGGSGQGGPGRGGSRQGGPPGAQRMYKQSMAQRARTMAIYNPIPVRQNCLTVNRSLFLFSEDNIVRKYAKKITEWPPFEWMILTTIIANCIVLALEQHLPVGDKTPLSERLEETEPYFIAIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSSVGSELDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGVLLFVAILMFAIIGLEFYMGKFHTTCFNTITGEMYDERPCGARTCDANNTQCQGYWLGPNYGITQFDNVLFAVLTVFQCITMEGWTDMLYYSNDALGSAWNWMYYVPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILAEDDNDTGSRRRPTLKKNKADLLNPQEGVDHMGDSVGSPFARGSMSGKGDETPFSKKERRLRFLIRKMVKTQAFYWTVISLVALNTLCVAVVHYDQPELLSDFLYYAEFIFLGLFMSEMLIKMYGLGTRPYFHSSFNCFDCAVIVGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFELGTPPTNFDTFPAAIMTVFQILTGEDWNVVMYDGIKSQGGVDKGMIFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEATTQKMALQKAKEVAEVSPLSAANLSIAAKEQQKNSKGSKSVWEQRTSEIRRQNLMTSREALYNELDAEDHWKMNYRNRPDVKTHLDRPLVVNPQENRNNNTNKPRPDDPNPQERSEEHPSRSHHHHSHHHHHHHHHTHHSSRQSLHLPELAETGFECDVEGAGEHRRPRHHQHRRRDEDGGERGRKHRDHHSRGETAGGDAEDGQEKRQRRHRHANHGDGDGRRERSRLHRNKKEGSLSTARPIQPGLPHTESQYSEDLDNLRNSSKMAIHDPDPDLYDPYYDPEQANNLLNLRGPDLHDSSLILTDSSKLEHTAIDMPPIYPSLNAKLQVNKNANTEPAKEGEKKEEEEDDGGDEEGHKRIPPFSSCFILSTTNPFRRCCHYILTLRYFEMCILSVIAMSSIALAAEDPVSQDSERNKVLRYFDYVFTGVFTFEMLIKMVVLGLILHEGSYFRDLWNFLDFVVVSGALVAFAFTSFSGGSGKGKDISIIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFARDCRGEYLVYERDNEVKSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPDVLKHSVDSTYENRGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYSIMSLIALNTIILMMKYNGASDAYDKVLKNLNIVFTTFFFMESILKIIAFGVRNFFRDAWNIFDFVSVIGSITDILVTELVNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAILFFIYAIIGMQLFGNLALDDEGAINEHNNFRTFFMALMLLFRSATGEAWHDIMLSCLGGMPCEPEEVGSECGSNVAYAYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKKLQALREEQNRTPLMFQRMEPSPNQEGGPGLNGLPELPQEPVNSLPPEVGMNESQSWVTSRAQEMCQKSVPENWNPDRPYPDDHHDSRHNPQTVEMREFGPVGDGYSDTEPYHPMEGHGRTISMPRLSADNQRRRHRPRGSNLSTITDTSPMRRSTSSLVHGRSGRGVRLDDYSLERVVSEEGHRHAPRRRDRDRDRDRDRSHRTSERSLARYTDADTGLGTDLSTTTQSGDLPPKERERERGRAKDRRHHHHHHHHHGSVDKDRYSHERDYHRHPQDRSDRQWSRSPSEGRGHRQ

Target ORF information:

RefSeq Version	XM_021479655.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479655.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGGCGCAT TCATTATAAG 
GATATGTACA GTTTATTGCG GGTTATCGAC CCCCCGCTGG GCTTAGGCAA GAAATGTCCT 
CATAGGGTGG CCTGCAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCAGCGACA 
GACCTGACAG TGGGCAAGAT CTACGCCGCC ATGATGATCA TGGAGTACTA CCGGCAGAGC 
AAGGCCAAAA AGCTGCAGGC GCTTCGTGAG GAGCAGAACC GAACGCCATT AATGTTTCAG 
CGCATGGAGC CGTCCCCAAA CCAGGAAGGG GGACCGGGGC TGAATGGCCT GCCCGAGCTG 
CCGCAGGAGC CCGTTAACAG CCTGCCTCCA GAGGTTGGAA TGAATGAAAG CCAGTCATGG 
GTGACGTCAC GCGCCCAGGA GATGTGCCAG AAGTCCGTTC CAGAAAACTG GAACCCTGAC 
AGACCATATC CTGATGACCA TCATGACAGC CGCCACAATC CACAGACGGT AGAAATGAGA 
GAGTTTGGGC CTGTTGGGGA CGGTTACTCG GACACTGAGC CCTATCACCC AATGGAAGGG 
CATGGCCGGA CCATCTCCAT GCCTCGCCTC TCTGCAGACA ACCAACGACG GAGACACAGG 
CCGCGAGGAA GTAATCTCAG TACCATCACC GACACAAGTC CAATGCGTCG TTCTACATCC 
TCATTGGTCC ATGGACGTTC AGGTCGAGGC GTGCGATTGG ATGATTACTC TCTGGAACGG 
GTGGTGTCAG AAGAGGGTCA CCGACATGCT CCCCGAAGGA GAGATCGTGA TCGAGACCGG 
GATAGAGATC GCAGTCATCG AACCTCAGAG AGATCCCTCG CTCGCTATAC AGATGCAGAC 
ACAGGTCTGG GTACAGATCT TAGCACCACT ACACAGTCGG GTGACCTCCC ACCTAAAGAA 
AGGGAACGAG AACGGGGTCG AGCAAAAGAC CGTCGCCACC ACCACCACCA TCATCACCAC 
CACGGCTCGG TGGATAAAGA TCGGTACAGT CACGAGCGCG ACTACCATCG TCATCCTCAA 
GACAGATCAG ATCGCCAATG GTCCCGCTCT CCGAGTGAGG GCAGAGGGCA CAGACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ab ( XM_021479656.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335331.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X16, mRNA.

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CloneID	ODa147908
Clone ID Related Accession (Same CDS sequence)	XM_021479656.1
Accession Version	XM_021479656.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335331.1
CDS	1445..6526
Translation	MARFGDEVPARYGGGGGGGSGQGGPGRGGSRQGGPPGAQRMYKQSMAQRARTMAIYNPIPVRQNCLTVNRSLFLFSEDNIVRKYAKKITEWPPFEWMILTTIIANCIVLALEQHLPVGDKTPLSERLEETEPYFIAIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSSVGSELDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGVLLFVAILMFAIIGLEFYMGKFHTTCFNTITGEMYDERPCGARTCDANNTQCQGYWLGPNYGITQFDNVLFAVLTVFQCITMEGWTDMLYYSNDALGSAWNWMYYVPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILAEDDNDTGSRRRPTLKKNKADLLNPQEGVDHMGDSVGSPFARGSMSGKGDETPFSKKERRLRFLIRKMVKTQAFYWTVISLVALNTLCVAVVHYDQPELLSDFLYYAEFIFLGLFMSEMLIKMYGLGTRPYFHSSFNCFDCAVIVGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFELGTPPTNFDTFPAAIMTVFQILTGEDWNVVMYDGIKSQGGVDKGMIFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEATTQKMALQKAKEVAEVSPLSAANLSIAAKEQQKNSKGSKSVWEQRTSEIRRQNLMTSREALYNELDAEDHWKMNYRNRPDVKTHLDRPLVVNPQENRNNNTNKPRPDDPNPQERSEEHPSRSHHHHSHHHHHHHHHTHHSSRQSLHLPELAETGFECDVEGAGEHRRPRHHQHRRRDEDGGERGRKHRDHHSRGETAGGDAEDGQEKRQRRHRHANHGDGDGRRERSRLHRNKKEGSLSTARPIQPGLPHTESQYSEDLDNLRNSSKMAIHDPDPDLYDPYYDPEQANNLLNLRGPDLHDSSLILTDSSKLEHTAIDMPPIYPSLNAKLQVNKNANTEPAKEGEKKEEEEDDGGDEEGHKRIPPFSSCFILSTTNPFRRCCHYILTLRYFEMCILSVIAMSSIALAAEDPVSQDSERNKVLRYFDYVFTGVFTFEMLIKMVVLGLILHEGSYFRDLWNFLDFVVVSGALVAFAFTSFSGGSGKGKDISIIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFARDCRGEYLVYERDNEVKSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPDVLKHSVDSTYENRGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYSIMSLIALNTIILMMKYNGASDAYDKVLKNLNIVFTTFFFMESILKIIAFGVRNFFRDAWNIFDFVSVIGSITDILVTELVNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAILFFIYAIIGMQLFGNLALDDEGAINEHNNFRTFFMALMLLFRSATGEAWHDIMLSCLGGMPCEPEEVGSECGSNVAYAYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAA

Target ORF information:

RefSeq Version	XM_021479656.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479656.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGACGA AGTGCCGGCC AGGTACGGCG GCGGCGGCGG CGGGGGCTCC 
GGTCAGGGGG GACCCGGCCG CGGCGGCAGC AGGCAGGGCG GCCCGCCAGG GGCCCAGAGG 
ATGTACAAGC AATCGATGGC CCAGAGAGCC CGGACCATGG CCATCTACAA CCCTATCCCG 
GTCCGACAGA ACTGCCTGAC GGTCAACCGC TCTCTCTTTC TCTTCAGTGA AGACAACATA 
GTGAGAAAAT ACGCCAAAAA GATCACCGAA TGGCCTCCGT TTGAGTGGAT GATTTTGACC 
ACCATCATCG CCAACTGCAT CGTACTGGCT CTCGAACAAC ACTTACCAGT TGGAGATAAA 
ACTCCACTGT CAGAACGACT GGAAGAGACG GAGCCCTATT TCATTGCTAT TTTCTGTTTT 
GAGTCGGGCA TTAAGATTCT GGCGCTGGGA TTCGCCTTCC ACAAGGGCTC CTACCTGAGA 
AACGGCTGGA ACGTCATGGA TTTTGTTGTG GTGCTCACAG GTATCCTGTC CTCCGTGGGG 
TCAGAATTAG ACCTGCGGAC GCTGCGAGCA GTCAGAGTGT TACGGCCGCT AAAACTGGTG 
TCAGGAATTC CTAGCCTGCA GGTGGTGCTG AAGTCCATCA TGAAGGCCAT GATTCCACTG 
CTGCAAATTG GTGTGCTTTT ATTCGTGGCC ATTCTTATGT TTGCCATTAT TGGCCTGGAG 
TTCTACATGG GCAAATTCCA CACCACCTGC TTCAACACCA TCACTGGTGA GATGTATGAT 
GAACGGCCAT GTGGAGCTCG GACATGTGAT GCAAACAATA CGCAATGCCA GGGCTACTGG 
CTTGGGCCCA ACTATGGGAT CACACAGTTC GACAATGTAC TTTTTGCTGT GCTCACTGTA 
TTCCAGTGCA TCACAATGGA GGGCTGGACT GATATGCTTT ATTATAGCAA TGATGCCTTA 
GGGAGTGCCT GGAACTGGAT GTACTATGTT CCGCTCATCA TTATTGGATC CTTCTTCATG 
CTTAATCTGG TGTTGGGTGT TCTCTCAGGT GAGTTTGCCA AAGAAAGAGA GCGTGTAGAG 
AACAGGAGTG AGTTCCTCAA GCTCAAACGG CAGCAGCAGA TCGAGAGAGA GCTCAACGGT 
TACCTGGAGT GGATCTGCAA AGCAGAAGAG GTCATACTCG CAGAGGATGA CAATGACACC 
GGATCTCGAC GAAGACCCAC ATTAAAGAAA AATAAAGCTG ACCTGTTAAA TCCACAAGAG 
GGAGTGGACC ACATGGGAGA TTCAGTTGGC TCACCTTTTG CTCGTGGAAG CATGAGCGGG 
AAGGGTGATG AAACCCCATT TAGCAAGAAA GAGCGACGTC TTCGCTTCCT TATTCGAAAG 
ATGGTAAAGA CTCAGGCGTT TTACTGGACC GTCATTAGCC TGGTTGCACT CAACACACTG 
TGTGTGGCTG TAGTACACTA CGATCAGCCT GAGCTACTGT CAGACTTCCT CTATTATGCT 
GAGTTCATTT TCCTAGGCCT CTTTATGTCA GAGATGCTGA TTAAGATGTA CGGACTGGGG 
ACGCGCCCTT ATTTCCACTC CTCATTCAAC TGTTTCGATT GTGCCGTCAT CGTTGGCAGT 
ATATTCGAGG TGGTTTGGGC CATGGTGAAA CCCGGGACGT CCTTTGGAAT CAGCGTGTTA 
CGAGCTCTAA GACTGCTTAG GATCTTCAAA GTCACCAAAT ACTGGGCATC GTTGCGCAAT 
TTAGTAGTGT CTCTTCTGAA CTCAATGAAG TCCATTATCA GTTTACTGTT CCTTCTCTTC 
CTATTCATCG TGGTCTTCGC TCTTCTTGGC ATGCAGCTCT TTGGTGGACA GTTTAATTTT 
GAACTGGGCA CTCCTCCTAC AAACTTTGAT ACTTTCCCCG CTGCCATTAT GACAGTGTTT 
CAGATTCTAA CAGGAGAGGA TTGGAATGTG GTGATGTATG ACGGCATCAA GTCTCAGGGT 
GGTGTGGACA AAGGAATGAT CTTCTCCGTA TTTTTCATTG TGCTCACACT TTTTGGCAAC 
TACACACTAC TGAATGTATT CTTGGCTATC GCTGTGGACA ACCTGGCCAA CGCTCAGGAA 
CTAACTAAGG ATGAGCAAGA GGAAGAAGAG GCCACAACAC AAAAGATGGC TCTTCAGAAA 
GCGAAAGAAG TGGCAGAAGT GAGCCCGCTG TCTGCTGCAA ACCTCTCGAT CGCTGCTAAA 
GAGCAGCAGA AGAACTCGAA AGGTTCGAAG TCAGTGTGGG AGCAGCGCAC AAGTGAGATC 
CGCAGACAGA ACCTGATGAC CAGCAGAGAG GCACTCTATA ATGAACTTGA TGCTGAAGAT 
CACTGGAAGA TGAATTACCG CAACCGGCCT GACGTGAAGA CCCACTTGGA CCGTCCACTA 
GTTGTCAATC CTCAGGAGAA CCGCAACAAC AACACCAACA AGCCCCGACC CGATGACCCA 
AACCCCCAGG AGCGAAGCGA AGAACACCCG TCCAGATCCC ATCACCATCA CAGCCACCAT 
CACCACCACC ACCACCACCA CACTCACCAC TCCAGCCGCC AGTCTCTGCA CCTCCCAGAG 
CTGGCCGAGA CCGGTTTTGA ATGTGATGTG GAGGGCGCAG GTGAGCACAG GCGACCCCGA 
CACCACCAGC ACAGGCGGAG GGATGAAGAT GGAGGAGAGA GAGGAAGGAA ACACAGAGAT 
CATCACAGCA GAGGAGAGAC GGCTGGAGGA GATGCAGAGG ACGGACAGGA GAAGCGGCAG 
AGGAGACATC GACATGCTAA CCATGGGGAT GGAGACGGGA GAAGGGAGAG GAGTCGACTG 
CATCGCAATA AGAAAGAGGG TAGTTTATCG ACGGCTCGTC CAATTCAGCC AGGACTACCT 
CACACTGAGA GCCAGTATAG CGAGGATCTG GACAACCTGC GCAACAGCAG CAAGATGGCC 
ATTCACGACC CTGACCCTGA CCTCTATGAC CCCTACTATG ACCCAGAGCA GGCCAATAAC 
CTGCTGAACC TGCGTGGACC AGATCTACAT GACAGCAGTC TCATTCTGAC AGACTCCAGC 
AAACTGGAGC ACACTGCCAT AGACATGCCC CCCATTTACC CCTCACTCAA TGCCAAGCTA 
CAAGTGAACA AGAATGCCAA CACAGAGCCA GCTAAAGAAG GGGAGAAGAA AGAGGAGGAA 
GAAGACGATG GTGGCGATGA AGAAGGCCAC AAGCGCATTC CACCTTTTAG TTCTTGTTTC 
ATCCTATCCA CAACCAACCC CTTCCGGAGA TGCTGTCACT ACATCCTCAC TCTACGTTAT 
TTCGAGATGT GTATTCTGTC CGTTATCGCT ATGAGCAGCA TCGCGTTAGC AGCAGAAGAT 
CCCGTCTCAC AGGATTCAGA GAGAAACAAA GTTTTGCGTT ATTTTGACTA CGTCTTTACT 
GGAGTGTTCA CCTTTGAGAT GCTTATCAAG ATGGTGGTGT TAGGCTTGAT TTTGCACGAG 
GGCTCATATT TTCGTGACCT GTGGAACTTT CTGGACTTTG TAGTGGTTAG TGGTGCCCTG 
GTAGCCTTTG CCTTCACGAG CTTCTCTGGG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ab ( XM_017358346.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213835.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X2, mRNA.

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CloneID	ODa54361
Clone ID Related Accession (Same CDS sequence)	XM_017358346.1
Accession Version	XM_017358346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTTGA CCACCATCAT CGCCAACTGC ATCGTACTGG CTCTCGAACA ACACTTACCA 
GTTGGAGATA AAACTCCACT GTCAGAACGA CTGGAAGAGA CGGAGCCCTA TTTCATTGCT 
ATTTTCTGTT TTGAGTCGGG CATTAAGATT CTGGCGCTGG GATTCGCCTT CCACAAGGGC 
TCCTACCTGA GAAACGGCTG GAACGTCATG GATTTTGTTG TGGTGCTCAC AGGTATCCTG 
TCCTCCGTGG GGTCAGAATT AGACCTGCGG ACGCTGCGAG CAGTCAGAGT GTTACGGCCG 
CTAAAACTGG TGTCAGGAAT TCCTAGCCTG CAGGTGGTGC TGAAGTCCAT CATGAAGGCC 
ATGATTCCAC TGCTGCAAAT TGGTGTGCTT TTATTCGTGG CCATTCTTAT GTTTGCCATT 
ATTGGCCTGG AGTTCTACAT GGGCAAATTC CACACCACCT GCTTCAACAC CATCACTGGT 
GAGATGTATG ATGAACGGCC ATGTGGAGCT CGGACATGTG ATGCAAACAA TACGCAATGC 
CAGGGCTACT GGCTTGGGCC CAACTATGGG ATCACACAGT TCGACAATGT ACTTTTTGCT 
GTGCTCACTG TATTCCAGTG CATCACAATG GAGGGCTGGA CTGATATGCT TTATTATAGC 
AATGATGCCT TAGGGAGTGC CTGGAACTGG ATGTACTATG TTCCGCTCAT CATTATTGGA 
TCCTTCTTCA TGCTTAATCT GGTGTTGGGT GTTCTCTCAG GTGAGTTTGC CAAAGAAAGA 
GAGCGTGTAG AGAACAGGAG TGAGTTCCTC AAGCTCAAAC GGCAGCAGCA GATCGAGAGA 
GAGCTCAACG GTTACCTGGA GTGGATCTGC AAAGCAGAAG AGGTCATACT CGCAGAGGAT 
GACAATGACA CCGGATCTCG ACGAAGACCC ACATTAAAGA AAAATAAAGC TGACCTGTTA 
AATCCACAAG AGGGAGTGGA CCACATGGGA GATTCAGTTG GCTCACCTTT TGCTCGTGGA 
AGCATGAGCG GGAAGGGTGA TGAAACCCCA TTTAGCAAGA AAGAGCGACG TCTTCGCTTC 
CTTATTCGAA AGATGGTAAA GACTCAGGCG TTTTACTGGA CCGTCATTAG CCTGGTTGCA 
CTCAACACAC TGTGTGTGGC TGTAGTACAC TACGATCAGC CTGAGCTACT GTCAGACTTC 
CTCTATTATG CTGAGTTCAT TTTCCTAGGC CTCTTTATGT CAGAGATGCT GATTAAGATG 
TACGGACTGG GGACGCGCCC TTATTTCCAC TCCTCATTCA ACTGTTTCGA TTGTGCCGTC 
ATCGTTGGCA GTATATTCGA GGTGGTTTGG GCCATGGTGA AACCCGGGAC GTCCTTTGGA 
ATCAGCGTGT TACGAGCTCT AAGACTGCTT AGGATCTTCA AAGTCACCAA ATACTGGGCA 
TCGTTGCGCA ATTTAGTAGT GTCTCTTCTG AACTCAATGA AGTCCATTAT CAGTTTACTG 
TTCCTTCTCT TCCTATTCAT CGTGGTCTTC GCTCTTCTTG GCATGCAGCT CTTTGGTGGA 
CAGTTTAATT TTGAACTGGG CACTCCTCCT ACAAACTTTG ATACTTTCCC CGCTGCCATT 
ATGACAGTGT TTCAGATTCT AACAGGAGAG GATTGGAATG TGGTGATGTA TGACGGCATC 
AAGTCTCAGG GTGGTGTGGA CAAAGGAATG ATCTTCTCCG TATTTTTCAT TGTGCTCACA 
CTTTTTGGCA ACTACACACT ACTGAATGTA TTCTTGGCTA TCGCTGTGGA CAACCTGGCC 
AACGCTCAGG AACTAACTAA GGATGAGCAA GAGGAAGAAG AGGCCACAAC ACAAAAGATG 
GCTCTTCAGA AAGCGAAAGA AGTGGCAGAA GTGAGCCCGC TGTCTGCTGC AAACCTCTCG 
ATCGCTGCTA AAGAGCAGCA GAAGAACTCG AAAGGTTCGA AGTCAGTGTG GGAGCAGCGC 
ACAAGTGAGA TCCGCAGACA GAACCTGATG ACCAGCAGAG AGGCACTCTA TAATGAACTT 
GATGCTGAAG ATCACTGGAA GATGAATTAC CGCAACCGGC CTGACGTGAA GACCCACTTG 
GACCGTCCAC TAGTTGTCAA TCCTCAGGAG AACCGCAACA ACAACACCAA CAAGCCCCGA 
CCCGATGACC CAAACCCCCA GGAGCGAAGC GAAGAACACC CGTCCAGATC CCATCACCAT 
CACAGCCACC ATCACCACCA CCACCACCAC CACACTCACC ACTCCAGCCG CCAGTCTCTG 
CACCTCCCAG AGCTGGCCGA GACCGGTTTT GAATGTGATG TGGAGGGCGC AGGTGAGCAC 
AGGCGACCCC GACACCACCA GCACAGGCGG AGGGATGAAG ATGGAGGAGA GAGAGGAAGG 
AAACACAGAG ATCATCACAG CAGAGGAGAG ACGGCTGGAG GAGATGCAGA GGACGGACAG 
GAGAAGCGGC AGAGGAGACA TCGACATGCT AACCATGGGG ATGGAGACGG GAGAAGGGAG 
AGGAGTCGAC TGCATCGCAA TAAGAAAGAG GGTAGTTTAT CGACGGCTCG TCCAATTCAG 
CCAGGACTAC CTCACACTGA GAGCCAGTAT AGCGAGGATC TGGACAACCT GCGCAACAGC 
AGCAAGATGG CCATTCACGA CCCTGACCCT GACCTCTATG ACCCCTACTA TGACCCAGAG 
CAGGCCAATA ACCTGCTGAA CCTGCGTGGA CCAGATCTAC ATGACAGCAG TCTCATTCTG 
ACAGACTCCA GCAAACTGGA GCACACTGCC ATAGACATGC CCCCCATTTA CCCCTCACTC 
AATGCCAAGC TACAAGTGAA CAAGAATGCC AACACAGAGC CAGCTAAAGA AGGGGAGAAG 
AAAGAGGAGG AAGAAGACGA TGGTGGCGAT GAAGAAGGCC ACAAGCGCAT TCCACCTTTT 
AGTTCTTGTT TCATCCTATC CACAACCAAC CCCTTCCGGA GATGCTGTCA CTACATCCTC 
ACTCTACGTT ATTTCGAGAT GTGTATTCTG TCCGTTATCG CTATGAGCAG CATCGCGTTA 
GCAGCAGAAG ATCCCGTCTC ACAGGATTCA GAGAGAAACA AAGTTTTGCG TTATTTTGAC 
TACGTCTTTA CTGGAGTGTT CACCTTTGAG ATGCTTATCA AGATGGTGGT GTTAGGCTTG 
ATTTTGCACG AGGGCTCATA TTTTCGTGAC CTGTGGAACT TTCTGGACTT TGTAGTGGTT 
AGTGGTGCCC TGGTAGCCTT TGCCTTCACG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGGCGCAT TCATTATAAG 
GATATGTACA GTTTATTGCG GGTTATCGAC CCCCCGCTGG GCTTAGGCAA GAAATGTCCT 
CATAGGGTGG CCTGCAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCGACAGAC 
CTGACAGTGG GCAAGATCTA CGCCGCCATG ATGATCATGG AGTACTACCG GCAGAGCAAG 
GCCAAAAAGC TGCAGGCGCT TCGTGAGGAG CAGAACCGAA CGCCATTAAT GTTTCAGCGC 
ATGGAGCCGT CCCCAAACCA GGAAGGGGGA CCGGGGCTGA ATGGCCTGCC CGAGCTGCCG 
CAGGAGCCCG TTAACAGCCT GCCTCCAGAG GTTGGAATGA ATGAAAGCCA GTCATGGGTG 
ACGTCACGCG CCCAGGAGAT GTGCCAGAAG TCCGTTCCAG AAAACTGGAA CCCTGACAGA 
CCATATCCTG ATGACCATCA TGACAGCCGC CACAATCCAC AGACGGTAGA AATGAGAGAG 
TTTGGGCCTG TTGGGGACGG TTACTCGGAC ACTGAGCCCT ATCACCCAAT GGAAGGGCAT 
GGCCGGACCA TCTCCATGCC TCGCCTCTCT GCAGACAACC AACGACGGAG ACACAGGCCG 
CGAGGAAGTA ATCTCAGTAC CATCACCGAC ACAAGTCCAA TGCGTCGTTC TACATCCTCA 
TTGGTCCATG GACGTTCAGG TCGAGGCGTG CGATTGGATG ATTACTCTCT GGAACGGGTG 
GTGTCAGAAG AGGGTCACCG ACATGCTCCC CGAAGGAGAG ATCGTGATCG AGACCGGGAT 
AGAGATCGCA GTCATCGAAC CTCAGAGAGA TCCCTCGCTC GCTATACAGA TGCAGACACA 
GGTCTGGGTA CAGATCTTAG CACCACTACA CAGTCGGGTG ACCTCCCACC TAAAGAAAGG 
GAACGAGAAC GGGGTCGAGC AAAAGACCGT CGCCACCACC ACCACCATCA TCACCACCAC 
GGCTCGGTGG ATAAAGATCG GTACAGTCAC GAGCGCGACT ACCATCGTCA TCCTCAAGAC 
AGATCAGATC GCCAATGGTC CCGCTCTCCG AGTGAGGGCA GAGGGCACAG ACAGGGCAGT 
AGTTCTGTGA GCGGAAGCCC CGTTCCCTCG ACGTCGGGGA CCAGCACCCC CCGGCGTGGC 
CGTCGCCAGT TGCCCCAGAC CCCATCCACT CCACGCCCTC ACGTCACCTA CTCCCCTGCG 
GTCCGCAAGC CTCCATATGG CTCCCCAGGC CAGGGTCGAC TCCGCTCCCC TTCCCCGCGA 
AACTTTTCTC CGCCAGGTCA TGAACCGCCA TACCACCGTC CACCCTCCCG CCATGCCTCA 
CCTCATCCTG GCTCACCCCG GCATGCCTCT CCGCGTTCCC CCAGGCACAC GTCGCCCAGG 
TCCCCTCGTC ACGGCCGCTG GGGCCCTCCG GACAGCCTAG AAGGTGATGG AACTTTCTAC 
GAACGGGACT ATGAGTACGA GCGCCACCAT GAGCCGCCAG CCTACGAACA GAGTTTATCA 
CAGGGCAACC CCCATCCACA TTCACACCCT CATCCCCGGT CACCAAGGAC TGCTCGTCAC 
GGGCCTCAAC CACACCCGCG GCGTATGCCT AACGGCTACC GTTCCTCCTC GCCCTCCCCG 
CACCGGCGCG GCCCCCACCC AGGCCCACAC CGGCCACCGC ATGGCCGTGG GCCCCGGAAG 
GGGTTGCACG AACAATATAG CGAGACGGAC GAGGATGATT GGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213835.1
CDS	9..6716
Translation	MILTTIIANCIVLALEQHLPVGDKTPLSERLEETEPYFIAIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSSVGSELDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGVLLFVAILMFAIIGLEFYMGKFHTTCFNTITGEMYDERPCGARTCDANNTQCQGYWLGPNYGITQFDNVLFAVLTVFQCITMEGWTDMLYYSNDALGSAWNWMYYVPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILAEDDNDTGSRRRPTLKKNKADLLNPQEGVDHMGDSVGSPFARGSMSGKGDETPFSKKERRLRFLIRKMVKTQAFYWTVISLVALNTLCVAVVHYDQPELLSDFLYYAEFIFLGLFMSEMLIKMYGLGTRPYFHSSFNCFDCAVIVGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFELGTPPTNFDTFPAAIMTVFQILTGEDWNVVMYDGIKSQGGVDKGMIFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEATTQKMALQKAKEVAEVSPLSAANLSIAAKEQQKNSKGSKSVWEQRTSEIRRQNLMTSREALYNELDAEDHWKMNYRNRPDVKTHLDRPLVVNPQENRNNNTNKPRPDDPNPQERSEEHPSRSHHHHSHHHHHHHHHTHHSSRQSLHLPELAETGFECDVEGAGEHRRPRHHQHRRRDEDGGERGRKHRDHHSRGETAGGDAEDGQEKRQRRHRHANHGDGDGRRERSRLHRNKKEGSLSTARPIQPGLPHTESQYSEDLDNLRNSSKMAIHDPDPDLYDPYYDPEQANNLLNLRGPDLHDSSLILTDSSKLEHTAIDMPPIYPSLNAKLQVNKNANTEPAKEGEKKEEEEDDGGDEEGHKRIPPFSSCFILSTTNPFRRCCHYILTLRYFEMCILSVIAMSSIALAAEDPVSQDSERNKVLRYFDYVFTGVFTFEMLIKMVVLGLILHEGSYFRDLWNFLDFVVVSGALVAFAFTGSGKGKDISIIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFARDCRGEYLVYERDNEVKSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPDVLKHSVDSTYENRGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYSIMSLIALNTIILMMKYNGASDAYDKVLKNLNIVFTTFFFMESILKIIAFGVRNFFRDAWNIFDFVSVIGSITDILVTELVNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAILFFIYAIIGMQLFGNLALDDEGAINEHNNFRTFFMALMLLFRSATGEAWHDIMLSCLGGMPCEPEEVGSECGSNVAYAYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQALREEQNRTPLMFQRMEPSPNQEGGPGLNGLPELPQEPVNSLPPEVGMNESQSWVTSRAQEMCQKSVPENWNPDRPYPDDHHDSRHNPQTVEMREFGPVGDGYSDTEPYHPMEGHGRTISMPRLSADNQRRRHRPRGSNLSTITDTSPMRRSTSSLVHGRSGRGVRLDDYSLERVVSEEGHRHAPRRRDRDRDRDRDRSHRTSERSLARYTDADTGLGTDLSTTTQSGDLPPKERERERGRAKDRRHHHHHHHHHGSVDKDRYSHERDYHRHPQDRSDRQWSRSPSEGRGHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPSTPRPHVTYSPAVRKPPYGSPGQGRLRSPSPRNFSPPGHEPPYHRPPSRHASPHPGSPRHASPRSPRHTSPRSPRHGRWGPPDSLEGDGTFYERDYEYERHHEPPAYEQSLSQGNPHPHSHPHPRSPRTARHGPQPHPRRMPNGYRSSSPSPHRRGPHPGPHRPPHGRGPRKGLHEQYSETDEDDWC

Target ORF information:

RefSeq Version	XM_017358346.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b (cacna1ab), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358346.1

ORF Insert Sequence:

ATGATTTTGA CCACCATCAT CGCCAACTGC ATCGTACTGG CTCTCGAACA ACACTTACCA 
GTTGGAGATA AAACTCCACT GTCAGAACGA CTGGAAGAGA CGGAGCCCTA TTTCATTGCT 
ATTTTCTGTT TTGAGTCGGG CATTAAGATT CTGGCGCTGG GATTCGCCTT CCACAAGGGC 
TCCTACCTGA GAAACGGCTG GAACGTCATG GATTTTGTTG TGGTGCTCAC AGGTATCCTG 
TCCTCCGTGG GGTCAGAATT AGACCTGCGG ACGCTGCGAG CAGTCAGAGT GTTACGGCCG 
CTAAAACTGG TGTCAGGAAT TCCTAGCCTG CAGGTGGTGC TGAAGTCCAT CATGAAGGCC 
ATGATTCCAC TGCTGCAAAT TGGTGTGCTT TTATTCGTGG CCATTCTTAT GTTTGCCATT 
ATTGGCCTGG AGTTCTACAT GGGCAAATTC CACACCACCT GCTTCAACAC CATCACTGGT 
GAGATGTATG ATGAACGGCC ATGTGGAGCT CGGACATGTG ATGCAAACAA TACGCAATGC 
CAGGGCTACT GGCTTGGGCC CAACTATGGG ATCACACAGT TCGACAATGT ACTTTTTGCT 
GTGCTCACTG TATTCCAGTG CATCACAATG GAGGGCTGGA CTGATATGCT TTATTATAGC 
AATGATGCCT TAGGGAGTGC CTGGAACTGG ATGTACTATG TTCCGCTCAT CATTATTGGA 
TCCTTCTTCA TGCTTAATCT GGTGTTGGGT GTTCTCTCAG GTGAGTTTGC CAAAGAAAGA 
GAGCGTGTAG AGAACAGGAG TGAGTTCCTC AAGCTCAAAC GGCAGCAGCA GATCGAGAGA 
GAGCTCAACG GTTACCTGGA GTGGATCTGC AAAGCAGAAG AGGTCATACT CGCAGAGGAT 
GACAATGACA CCGGATCTCG ACGAAGACCC ACATTAAAGA AAAATAAAGC TGACCTGTTA 
AATCCACAAG AGGGAGTGGA CCACATGGGA GATTCAGTTG GCTCACCTTT TGCTCGTGGA 
AGCATGAGCG GGAAGGGTGA TGAAACCCCA TTTAGCAAGA AAGAGCGACG TCTTCGCTTC 
CTTATTCGAA AGATGGTAAA GACTCAGGCG TTTTACTGGA CCGTCATTAG CCTGGTTGCA 
CTCAACACAC TGTGTGTGGC TGTAGTACAC TACGATCAGC CTGAGCTACT GTCAGACTTC 
CTCTATTATG CTGAGTTCAT TTTCCTAGGC CTCTTTATGT CAGAGATGCT GATTAAGATG 
TACGGACTGG GGACGCGCCC TTATTTCCAC TCCTCATTCA ACTGTTTCGA TTGTGCCGTC 
ATCGTTGGCA GTATATTCGA GGTGGTTTGG GCCATGGTGA AACCCGGGAC GTCCTTTGGA 
ATCAGCGTGT TACGAGCTCT AAGACTGCTT AGGATCTTCA AAGTCACCAA ATACTGGGCA 
TCGTTGCGCA ATTTAGTAGT GTCTCTTCTG AACTCAATGA AGTCCATTAT CAGTTTACTG 
TTCCTTCTCT TCCTATTCAT CGTGGTCTTC GCTCTTCTTG GCATGCAGCT CTTTGGTGGA 
CAGTTTAATT TTGAACTGGG CACTCCTCCT ACAAACTTTG ATACTTTCCC CGCTGCCATT 
ATGACAGTGT TTCAGATTCT AACAGGAGAG GATTGGAATG TGGTGATGTA TGACGGCATC 
AAGTCTCAGG GTGGTGTGGA CAAAGGAATG ATCTTCTCCG TATTTTTCAT TGTGCTCACA 
CTTTTTGGCA ACTACACACT ACTGAATGTA TTCTTGGCTA TCGCTGTGGA CAACCTGGCC 
AACGCTCAGG AACTAACTAA GGATGAGCAA GAGGAAGAAG AGGCCACAAC ACAAAAGATG 
GCTCTTCAGA AAGCGAAAGA AGTGGCAGAA GTGAGCCCGC TGTCTGCTGC AAACCTCTCG 
ATCGCTGCTA AAGAGCAGCA GAAGAACTCG AAAGGTTCGA AGTCAGTGTG GGAGCAGCGC 
ACAAGTGAGA TCCGCAGACA GAACCTGATG ACCAGCAGAG AGGCACTCTA TAATGAACTT 
GATGCTGAAG ATCACTGGAA GATGAATTAC CGCAACCGGC CTGACGTGAA GACCCACTTG 
GACCGTCCAC TAGTTGTCAA TCCTCAGGAG AACCGCAACA ACAACACCAA CAAGCCCCGA 
CCCGATGACC CAAACCCCCA GGAGCGAAGC GAAGAACACC CGTCCAGATC CCATCACCAT 
CACAGCCACC ATCACCACCA CCACCACCAC CACACTCACC ACTCCAGCCG CCAGTCTCTG 
CACCTCCCAG AGCTGGCCGA GACCGGTTTT GAATGTGATG TGGAGGGCGC AGGTGAGCAC 
AGGCGACCCC GACACCACCA GCACAGGCGG AGGGATGAAG ATGGAGGAGA GAGAGGAAGG 
AAACACAGAG ATCATCACAG CAGAGGAGAG ACGGCTGGAG GAGATGCAGA GGACGGACAG 
GAGAAGCGGC AGAGGAGACA TCGACATGCT AACCATGGGG ATGGAGACGG GAGAAGGGAG 
AGGAGTCGAC TGCATCGCAA TAAGAAAGAG GGTAGTTTAT CGACGGCTCG TCCAATTCAG 
CCAGGACTAC CTCACACTGA GAGCCAGTAT AGCGAGGATC TGGACAACCT GCGCAACAGC 
AGCAAGATGG CCATTCACGA CCCTGACCCT GACCTCTATG ACCCCTACTA TGACCCAGAG 
CAGGCCAATA ACCTGCTGAA CCTGCGTGGA CCAGATCTAC ATGACAGCAG TCTCATTCTG 
ACAGACTCCA GCAAACTGGA GCACACTGCC ATAGACATGC CCCCCATTTA CCCCTCACTC 
AATGCCAAGC TACAAGTGAA CAAGAATGCC AACACAGAGC CAGCTAAAGA AGGGGAGAAG 
AAAGAGGAGG AAGAAGACGA TGGTGGCGAT GAAGAAGGCC ACAAGCGCAT TCCACCTTTT 
AGTTCTTGTT TCATCCTATC CACAACCAAC CCCTTCCGGA GATGCTGTCA CTACATCCTC 
ACTCTACGTT ATTTCGAGAT GTGTATTCTG TCCGTTATCG CTATGAGCAG CATCGCGTTA 
GCAGCAGAAG ATCCCGTCTC ACAGGATTCA GAGAGAAACA AAGTTTTGCG TTATTTTGAC 
TACGTCTTTA CTGGAGTGTT CACCTTTGAG ATGCTTATCA AGATGGTGGT GTTAGGCTTG 
ATTTTGCACG AGGGCTCATA TTTTCGTGAC CTGTGGAACT TTCTGGACTT TGTAGTGGTT 
AGTGGTGCCC TGGTAGCCTT TGCCTTCACG GGGAGCGGTA AGGGGAAAGA CATCAGTATC 
ATCAAGTCTC TGAGGGTTCT AAGGGTGCTG CGTCCACTCA AGACCATCAA ACGTCTTCCA 
AAACTAAAAG CGGTGTTTGA CTGTGTAGTA AACTCTCTGA AGAATGTGTT GAACATCCTC 
ATTGTCTACA TGCTGTTCAT GTTCATCTTC GCTGTGGTGG CCGTGCAGCT TTTTAAAGGA 
CGATTCTTTT ACTGCACTGA TGAGTCTAAA GAGTTTGCCC GAGACTGCAG GGGCGAATAT 
TTGGTGTACG AGCGCGATAA CGAGGTCAAA TCTCAGAAGA GGGAGTGGAA GAAGTACGAT 
TTCCACTACG ACAATGTGCT TTGGGCCCTG CTTACTCTCT TTACTGTGTC CACCGGAGAG 
GGATGGCCAG ATGTGTTGAA GCACTCTGTG GACTCCACTT ACGAGAATCG GGGCCCGAGC 
CCGGGATACA GGATGGAAAT GTCCATCTTT TACGTGGTGT ACTTCGTTGT CTTCCCCTTT 
TTCTTCGTAA ACATATTTGT AGCTCTCATC ATCATCACCT TCCAAGAGCA AGGAGACAAG 
ATGATGGAGG ACTACAGCCT CGAAAAAAAT GAGAGAGCCT GTATTGATTT TGCTATAAAT 
GCCAAACCTC TAACGCGCCA CATGCCTCAG AACAAGCAAA GCTTTCAGTA CCGAATGTGG 
CAGTTTGTGG TGTCGCCTCC GTTTGAGTAC AGTATCATGT CTCTCATTGC GCTCAACACC 
ATCATCCTTA TGATGAAGTA TAATGGGGCT TCCGATGCAT ATGACAAAGT CCTGAAGAAC 
CTGAATATAG TGTTCACCAC ATTCTTCTTC ATGGAGAGTA TTCTGAAAAT CATTGCCTTT 
GGTGTCAGAA ATTTTTTCAG AGATGCTTGG AATATTTTTG ATTTTGTGTC TGTTATCGGC 
AGCATCACTG ATATTTTAGT GACTGAGCTC GTGAATAACT TCATAAACCT GAGTTTTCTG 
AGACTCTTTC GAGCAGCTCG TCTCATTAAG CTCTTGCGGC AGGGCGAGAC CATTCGCATT 
CTGCTTTGGA CCTTCGTTCA GTCATTTAAG GCTCTGCCAT ATGTATGCCT GCTCATTGCA 
ATTCTGTTTT TCATCTACGC CATCATTGGC ATGCAGCTGT TTGGGAACTT GGCTCTGGAT 
GACGAAGGTG CTATCAATGA ACACAACAAC TTCAGAACCT TCTTCATGGC CCTCATGCTC 
CTCTTCAGGA GTGCCACGGG CGAGGCGTGG CATGATATCA TGCTGTCATG TCTGGGAGGA 
ATGCCCTGTG AGCCGGAGGA GGTTGGATCA GAATGTGGCA GCAATGTGGC CTATGCATAC 
TTCGTCTCTT TCATCTTTCT CTGTTCGTTC CTGATGCTGA ATCTTTTTGT GGCTGTCATC 
ATGGATAACT TTGAGTACTT GACCAGGGAC TCCTCCATCC TGGGTCCGCA TCATTTGGAT 
GAGTATGTCA GGATATGGGC AGAGTACGAC CCCGCAGCCT GCGGGCGCAT TCATTATAAG 
GATATGTACA GTTTATTGCG GGTTATCGAC CCCCCGCTGG GCTTAGGCAA GAAATGTCCT 
CATAGGGTGG CCTGCAAGAG ACTGCTCCGT ATGGACCTGC CCGTTGCTGA TGACAACACG 
GTGCATTTCA ACTCCACTCT GATGGCCTTG ATTCGCACAG CACTGGACAT CAAAATTGCC 
AAGGGAGGAG CGGACAAACA CCAGATGGAC GCGGAGCTCC GTAAAGAAAT GATGGCGATT 
TGGCCAAATC TCTCTCAGAA GAACCTGGAT CTGCTGGTAA CGCCACACAA GTCGACAGAC 
CTGACAGTGG GCAAGATCTA CGCCGCCATG ATGATCATGG AGTACTACCG GCAGAGCAAG 
GCCAAAAAGC TGCAGGCGCT TCGTGAGGAG CAGAACCGAA CGCCATTAAT GTTTCAGCGC 
ATGGAGCCGT CCCCAAACCA GGAAGGGGGA CCGGGGCTGA ATGGCCTGCC CGAGCTGCCG 
CAGGAGCCCG TTAACAGCCT GCCTCCAGAG GTTGGAATGA ATGAAAGCCA GTCATGGGTG 
ACGTCACGCG CCCAGGAGAT GTGCCAGAAG TCCGTTCCAG AAAACTGGAA CCCTGACAGA 
CCATATCCTG ATGACCATCA TGACAGCCGC CACAATCCAC AGACGGTAGA AATGAGAGAG 
TTTGGGCCTG TTGGGGACGG TTACTCGGAC ACTGAGCCCT ATCACCCAAT GGAAGGGCAT 
GGCCGGACCA TCTCCATGCC TCGCCTCTCT GCAGACAACC AACGACGGAG ACACAGGCCG 
CGAGGAAGTA ATCTCAGTAC CATCACCGAC ACAAGTCCAA TGCGTCGTTC TACATCCTCA 
TTGGTCCATG GACGTTCAGG TCGAGGCGTG CGATTGGATG ATTACTCTCT GGAACGGGTG 
GTGTCAGAAG AGGGTCACCG ACATGCTCCC CGAAGGAGAG ATCGTGATCG AGACCGGGAT 
AGAGATCGCA GTCATCGAAC CTCAGAGAGA TCCCTCGCTC GCTATACAGA TGCAGACACA 
GGTCTGGGTA CAGATCTTAG CACCACTACA CAGTCGGGTG ACCTCCCACC TAAAGAAAGG 
GAACGAGAAC GGGGTCGAGC AAAAGACCGT CGCCACCACC ACCACCATCA TCACCACCAC 
GGCTCGGTGG ATAAAGATCG GTACAGTCAC GAGCGCGACT ACCATCGTCA TCCTCAAGAC 
AGATCAGATC GCCAATGGTC CCGCTCTCCG AGTGAGGGCA GAGGGCACAG ACAGGGCAGT 
AGTTCTGTGA GCGGAAGCCC CGTTCCCTCG ACGTCGGGGA CCAGCACCCC CCGGCGTGGC 
CGTCGCCAGT TGCCCCAGAC CCCATCCACT CCACGCCCTC ACGTCACCTA CTCCCCTGCG 
GTCCGCAAGC CTCCATATGG CTCCCCAGGC CAGGGTCGAC TCCGCTCCCC TTCCCCGCGA 
AACTTTTCTC CGCCAGGTCA TGAACCGCCA TACCACCGTC CACCCTCCCG CCATGCCTCA 
CCTCATCCTG GCTCACCCCG GCATGCCTCT CCGCGTTCCC CCAGGCACAC GTCGCCCAGG 
TCCCCTCGTC ACGGCCGCTG GGGCCCTCCG GACAGCCTAG AAGGTGATGG AACTTTCTAC 
GAACGGGACT ATGAGTACGA GCGCCACCAT GAGCCGCCAG CCTACGAACA GAGTTTATCA 
CAGGGCAACC CCCATCCACA TTCACACCCT CATCCCCGGT CACCAAGGAC TGCTCGTCAC 
GGGCCTCAAC CACACCCGCG GCGTATGCCT AACGGCTACC GTTCCTCCTC GCCCTCCCCG 
CACCGGCGCG GCCCCCACCC AGGCCCACAC CGGCCACCGC ATGGCCGTGG GCCCCGGAAG 
GGGTTGCACG AACAATATAG CGAGACGGAC GAGGATGATT GGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	cacna1ab
Entrez Gene ID	569528
Full Name	calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b
General protein information	Preferred Names calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, b Names voltage-dependent P/Q-type calcium channel subunit alpha-1A fakir far unm tb204 unm_tb204
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Homo sapiens (human)	CACNA1A	NP_001120694.1
Pan troglodytes (chimpanzee)	CACNA1A	XP_003316202.1
Xenopus tropicalis (tropical clawed frog)	cacna1a	XP_004919196.1
	CACNA1A	XP_002801141.1
Danio rerio (zebrafish)	cacna1ab	XP_698014.6
Canis lupus familiaris (dog)	CACNA1A	XP_005632867.1
Bos taurus (cattle)	CACNA1A	NP_001068597.1
Mus musculus (house mouse)	Cacna1a	NP_031604.3
Rattus norvegicus (Norway rat)	Cacna1a	NP_037050.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

cacna1ab cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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