e2f7 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	e2f7
Entrez Gene ID	567941
Full Name	E2F transcription factor 7
Synonyms	E2F-7,fc43h03,si:dkey-217k21.2,wu:fc43h03
General protein information	Preferred Names E2F transcription factor 7 Names transcription factor E2F7
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001045147.2	NP_001038612.2	transcription factor E2F7

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	E2f7	NP_848724.2
Danio rerio (zebrafish)	e2f7	NP_001038612.2
Canis lupus familiaris (dog)	E2F7	XP_539692.4
Homo sapiens (human)	E2F7	NP_976328.2
Pan troglodytes (chimpanzee)	E2F7	XP_001161983.1
	E2F7	XP_001083107.1
Rattus norvegicus (Norway rat)	E2f7	NP_001101562.1
Gallus gallus (chicken)	E2F7	XP_416110.4
Xenopus tropicalis (tropical clawed frog)	e2f7	XP_002935684.2
Bos taurus (cattle)	E2F7	XP_003586087.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Genome-wide analysis reveals NRP1 as a direct HIF1?-E2F7 target in the regulation of motorneuron guidance in vivo.
de Bruin A, A Cornelissen PW, Kirchmaier BC, Mokry M, Iich E, Nirmala E, Liang KH, D V?gh AM, Scholman KT, Groot Koerkamp MJ, Holstege FC, Cuppen E, Schulte-Merker S, Bakker WJ
Nucleic acids research44(8)3549-66(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

E2F8 promotes hepatic steatosis through FABP3 expression in diet-induced obesity in zebrafish.
Shimada Y, Kuninaga S, Ariyoshi M, Zhang B, Shiina Y, Takahashi Y, Umemoto N, Nishimura Y, Enari H, Tanaka T
Nutrition & metabolism1217(2015)

Atypical E2fs control lymphangiogenesis through transcriptional regulation of Ccbe1 and Flt4.
Weijts BG, van Impel A, Schulte-Merker S, de Bruin A
PloS one8(9)e73693(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The HIF1alpha-E2F7 complex regulates axon guidance of spinal motor neurons through NRP1
Title: Genome-wide analysis reveals NRP1 as a direct HIF1?-E2F7 target in the regulation of motorneuron guidance in vivo.

The following e2f7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the e2f7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01492	NM_001045147.2 Latest version!	Danio rerio E2F transcription factor 7 (e2f7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

e2f7 ( NM_001045147.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038612.2 Danio rerio E2F transcription factor 7 (e2f7), mRNA.

Price & Availability↑

CloneID	ODa01492
Clone ID Related Accession (Same CDS sequence)	NM_001045147.2
Accession Version	NM_001045147.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	transcription factor E2F7
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX072538.10. On Mar 1, 2008 this sequence version replaced NM_001045147.1. ##Evidence-Data-START## Transcript exon combination :: GFIL01009117.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCAGGAGG TGAAGTGTTT AACATTAAAG GATTTGCTTG GTGTCAGAAC ACTGGTGAAT 
AAGACAGGCT CAGATGATGC TGCCAGCATG AATGACCATA AAGAAAACAT CTGCATGGAC 
AGAAGGAAAA TGACCCCACT GAAGAGCGAG TCTCTGACAG CTGCTTTGAA TGGACATGGG 
AAGATTTCCA GTCCTGAAAT CACCCACATA ACCCCTATTA AACTCACTGA GAAGGCCCAT 
CCGGACCCCT GGACGCCAAC AGCGAATCTG AAAATGCTGA TCAATGCTGC TAGTCCAGAT 
ATACGTGACA GAGAAATGAA GAAAACCCTC TTCAAACCCA TTGAAAATAA AGGGAAGATT 
GCAGAGGAGG AGGAAGAGGA AGAACTTGAT GACTCGTGTC AGTATGAAGC GTTAGACGAA 
TCTGAAAGGC GACCCAGTCG AAAACAGAAG AGTTTGGGGC TTTTGTGTCA GAAATTTTTG 
GCTTTGTACC CTGATTACCC AGAATCCTCT GAAAGCATCA ATATTTCCCT GGATGAAGTG 
GCCACATGCT TGGGCGTTGA GAGACGACGC ATCTATGATA TTGTGAATGT GCTGGAGTCT 
CTGATGCTGG TCAGCAGAAA GGCAAAAAAC ATGTACGTGT GGCACGGACG CTCGCGGCTC 
CCACAGACTC TACAGGGACT TTTACAGGCC GGTCGAGATC AACACTATGA TTTACTGATG 
GACCAGAGGG AAGGGAATGG CCTGCATGCT GTCCAACACG TGCAGAACGC ACACGCCGCC 
TCCAGCAGGA GAAAGGATAA ATCGCTCCGA ATCATGAGCC AGAAGTTTGT CATGCTGTTC 
CTGGTGTCCA AAACCCAAAC TGTAACCCTC GATATGGCTG CTAAGATTTT AATTGAAGAG 
GGTCAGGAAG AGTCCTACGA CAGCAAGTAT AAAACTAAGG TGCGGCGTTT GTATGATATC 
GCTAACGTCC TGACTAGCCT GAATCTGATC AAGAAAATTC ACATGAGAGA AGAAAAGACC 
CGTAAACCAG TCTTCAAATG GATCGGGCCT GGAAATTTTC AAAGCAGCAG CAATTCAGAT 
GATTTGAGAG GTCAAATCAG CAACTCCGGC ACTGAACGAC GGGAGAAAAT GGCACGTCAT 
TCTTCCTTCC AGGTCATTAC TGCCCCTCCT GTCAATCAAA GACTGATAAG CTCCGCCCCC 
AGCACGCCTC ACAGATATTC TACAGATGAG CCGGTGGATT ACTCCAGGAA AAGCGGGAAC 
AACAGTGCAG TGTGTCGACT GCAGTTTGGA GACGGTGTTC ATCCCAGTGT TAGTCCTGCA 
GTTCCCTCTG CACTGGCGTC TCTAGCGATG CCTCTTCAAG CTGATCTGAT GCCCGTTCCA 
GCTTCGTTCT CTCACCCGCT GGCAATCCTG CCGCAGACGC CCCTGCTCAT GCTGTACAGC 
GGGAACATTT CTGACGGTGC CTCCAGCCTG AGAAAGAGGG AGAGATCAGA GGAAGACGAC 
CATCAGACCA CAAAATGCAG AAGACGCTCG GCATCCATAG AAAGTGACAC AGTGGAGAGC 
GAGAGCTTGA GCTCCAGCAC CAGACGGTCT CCAGTCTGCT CTCCCGAAGG CTCTCCGTGG 
GACGAGGCCT CGTTCGGGGG TCTGCATGAA GATGATGTGG CTGCATCCAG TTCCAAAGAC 
GCCCTCCTGT CCCCTCATTA CCTCTATGTG CCAAACACTG CAGGTCTGAA TAGTTTTAAC 
TTCCTCCTGC CAGCAGGGCA CGCGCAGGGT GGAGTGCCGG CCGTAGCCAT GCCATATTTT 
GTGGTGCAAT CACCACTAAT AGCAGGTGCA ATGCCCACAT CCAGCACTGA AGGGGCTGCT 
GGCTTCAGTG TGCCCACCGT TCTGTCGCCC GCACAGTTTG TGATGGCAGG AGGAGCTTAT 
GGTGTGACTG AAATACTGCA GAGCCCAGAA CATCATGGGA ACGTACCAGC CACAACATCA 
TCACCACGAG CAGAAGAGAG CCCGAAACCA GCACAAACAC AGACTCCTGT GACACCAAAG 
GAAGCATCGC TGGGGTCCAA ATCTTTCTTC GAGACACCTG GGGCATTTGG GTCTCTCGTC 
AACCAATCAG CAGCTCGCAA ACGAGGCTCC GCCCAGAGGA GGCTGGACAT CGGGCACACC 
GCAGCCAATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038612.2
CDS	179..2350
Translation	MQEVKCLTLKDLLGVRTLVNKTGSDDAASMNDHKENICMDRRKMTPLKSESLTAALNGHGKISSPEITHITPIKLTEKAHPDPWTPTANLKMLINAASPDIRDREMKKTLFKPIENKGKIAEEEEEEELDDSCQYEALDESERRPSRKQKSLGLLCQKFLALYPDYPESSESINISLDEVATCLGVERRRIYDIVNVLESLMLVSRKAKNMYVWHGRSRLPQTLQGLLQAGRDQHYDLLMDQREGNGLHAVQHVQNAHAASSRRKDKSLRIMSQKFVMLFLVSKTQTVTLDMAAKILIEEGQEESYDSKYKTKVRRLYDIANVLTSLNLIKKIHMREEKTRKPVFKWIGPGNFQSSSNSDDLRGQISNSGTERREKMARHSSFQVITAPPVNQRLISSAPSTPHRYSTDEPVDYSRKSGNNSAVCRLQFGDGVHPSVSPAVPSALASLAMPLQADLMPVPASFSHPLAILPQTPLLMLYSGNISDGASSLRKRERSEEDDHQTTKCRRRSASIESDTVESESLSSSTRRSPVCSPEGSPWDEASFGGLHEDDVAASSSKDALLSPHYLYVPNTAGLNSFNFLLPAGHAQGGVPAVAMPYFVVQSPLIAGAMPTSSTEGAAGFSVPTVLSPAQFVMAGGAYGVTEILQSPEHHGNVPATTSSPRAEESPKPAQTQTPVTPKEASLGSKSFFETPGAFGSLVNQSAARKRGSAQRRLDIGHTAAN

Target ORF information:

RefSeq Version	NM_001045147.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio E2F transcription factor 7 (e2f7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045147.2

ORF Insert Sequence:

ATGCAGGAGG TGAAGTGTTT AACATTAAAG GATTTGCTTG GTGTCAGAAC ACTGGTGAAT 
AAGACAGGCT CAGATGATGC TGCCAGCATG AATGACCATA AAGAAAACAT CTGCATGGAC 
AGAAGGAAAA TGACCCCACT GAAGAGCGAG TCTCTGACAG CTGCTTTGAA TGGACATGGG 
AAGATTTCCA GTCCTGAAAT CACCCACATA ACCCCTATTA AACTCACTGA GAAGGCCCAT 
CCGGACCCCT GGACGCCAAC AGCGAATCTG AAAATGCTGA TCAATGCTGC TAGTCCAGAT 
ATACGTGACA GAGAAATGAA GAAAACCCTC TTCAAACCCA TTGAAAATAA AGGGAAGATT 
GCAGAGGAGG AGGAAGAGGA AGAACTTGAT GACTCGTGTC AGTATGAAGC GTTAGACGAA 
TCTGAAAGGC GACCCAGTCG AAAACAGAAG AGTTTGGGGC TTTTGTGTCA GAAATTTTTG 
GCTTTGTACC CTGATTACCC AGAATCCTCT GAAAGCATCA ATATTTCCCT GGATGAAGTG 
GCCACATGCT TGGGCGTTGA GAGACGACGC ATCTATGATA TTGTGAATGT GCTGGAGTCT 
CTGATGCTGG TCAGCAGAAA GGCAAAAAAC ATGTACGTGT GGCACGGACG CTCGCGGCTC 
CCACAGACTC TACAGGGACT TTTACAGGCC GGTCGAGATC AACACTATGA TTTACTGATG 
GACCAGAGGG AAGGGAATGG CCTGCATGCT GTCCAACACG TGCAGAACGC ACACGCCGCC 
TCCAGCAGGA GAAAGGATAA ATCGCTCCGA ATCATGAGCC AGAAGTTTGT CATGCTGTTC 
CTGGTGTCCA AAACCCAAAC TGTAACCCTC GATATGGCTG CTAAGATTTT AATTGAAGAG 
GGTCAGGAAG AGTCCTACGA CAGCAAGTAT AAAACTAAGG TGCGGCGTTT GTATGATATC 
GCTAACGTCC TGACTAGCCT GAATCTGATC AAGAAAATTC ACATGAGAGA AGAAAAGACC 
CGTAAACCAG TCTTCAAATG GATCGGGCCT GGAAATTTTC AAAGCAGCAG CAATTCAGAT 
GATTTGAGAG GTCAAATCAG CAACTCCGGC ACTGAACGAC GGGAGAAAAT GGCACGTCAT 
TCTTCCTTCC AGGTCATTAC TGCCCCTCCT GTCAATCAAA GACTGATAAG CTCCGCCCCC 
AGCACGCCTC ACAGATATTC TACAGATGAG CCGGTGGATT ACTCCAGGAA AAGCGGGAAC 
AACAGTGCAG TGTGTCGACT GCAGTTTGGA GACGGTGTTC ATCCCAGTGT TAGTCCTGCA 
GTTCCCTCTG CACTGGCGTC TCTAGCGATG CCTCTTCAAG CTGATCTGAT GCCCGTTCCA 
GCTTCGTTCT CTCACCCGCT GGCAATCCTG CCGCAGACGC CCCTGCTCAT GCTGTACAGC 
GGGAACATTT CTGACGGTGC CTCCAGCCTG AGAAAGAGGG AGAGATCAGA GGAAGACGAC 
CATCAGACCA CAAAATGCAG AAGACGCTCG GCATCCATAG AAAGTGACAC AGTGGAGAGC 
GAGAGCTTGA GCTCCAGCAC CAGACGGTCT CCAGTCTGCT CTCCCGAAGG CTCTCCGTGG 
GACGAGGCCT CGTTCGGGGG TCTGCATGAA GATGATGTGG CTGCATCCAG TTCCAAAGAC 
GCCCTCCTGT CCCCTCATTA CCTCTATGTG CCAAACACTG CAGGTCTGAA TAGTTTTAAC 
TTCCTCCTGC CAGCAGGGCA CGCGCAGGGT GGAGTGCCGG CCGTAGCCAT GCCATATTTT 
GTGGTGCAAT CACCACTAAT AGCAGGTGCA ATGCCCACAT CCAGCACTGA AGGGGCTGCT 
GGCTTCAGTG TGCCCACCGT TCTGTCGCCC GCACAGTTTG TGATGGCAGG AGGAGCTTAT 
GGTGTGACTG AAATACTGCA GAGCCCAGAA CATCATGGGA ACGTACCAGC CACAACATCA 
TCACCACGAG CAGAAGAGAG CCCGAAACCA GCACAAACAC AGACTCCTGT GACACCAAAG 
GAAGCATCGC TGGGGTCCAA ATCTTTCTTC GAGACACCTG GGGCATTTGG GTCTCTCGTC 
AACCAATCAG CAGCTCGCAA ACGAGGCTCC GCCCAGAGGA GGCTGGACAT CGGGCACACC 
GCAGCCAATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Genome-wide analysis reveals NRP1 as a direct HIF1?-E2F7 target in the regulation of motorneuron guidance in vivo.
Nucleic acids research44(8)3549-66(2016 05)
de Bruin A,A Cornelissen PW,Kirchmaier BC,Mokry M,Iich E,Nirmala E,Liang KH,D V?gh AM,Scholman KT,Groot Koerkamp MJ,Holstege FC,Cuppen E,Schulte-Merker S,Bakker WJ

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

E2F8 promotes hepatic steatosis through FABP3 expression in diet-induced obesity in zebrafish.
Nutrition & metabolism1217(2015)
Shimada Y,Kuninaga S,Ariyoshi M,Zhang B,Shiina Y,Takahashi Y,Umemoto N,Nishimura Y,Enari H,Tanaka T

Atypical E2fs control lymphangiogenesis through transcriptional regulation of Ccbe1 and Flt4.
PloS one8(9)e73693(2013)
Weijts BG,van Impel A,Schulte-Merker S,de Bruin A