ifit12 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ifit12
Entrez Gene ID	567504
Full Name	interferon-induced protein with tetratricopeptide repeats 12
Synonyms	IFIT12E,ifit5,si:dkey-26g8.8
General protein information	Preferred Names interferon-induced protein with tetratricopeptide repeats 12 Names interferon-induced protein with tetratricopeptide repeats 5-like interferon-induced protein with tetratricopeptide repeats 5
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001190465.1	NP_001177394.1	interferon-induced protein with tetratricopeptide repeats 5-like

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	si:dkey-26g8.11	XP_695829.2
Xenopus tropicalis (tropical clawed frog)	LOC100486481	XP_002937235.2
Xenopus tropicalis (tropical clawed frog)	LOC100486634	XP_002937236.1
Danio rerio (zebrafish)	ifit5	NP_001177394.1
Xenopus tropicalis (tropical clawed frog)	LOC100489506	XP_002944835.2
Bos taurus (cattle)	IFIT5	NP_001069166.1
Xenopus tropicalis (tropical clawed frog)	LOC100486954	XP_002937237.2
Homo sapiens (human)	IFIT5	NP_036552.1
	IFIT5	XP_001086665.1
Danio rerio (zebrafish)	LOC572297	XP_701096.4
Xenopus tropicalis (tropical clawed frog)	LOC101735168	XP_004916534.1
Pan troglodytes (chimpanzee)	IFIT5	XP_521554.3
Canis lupus familiaris (dog)	IFIT5	XP_005637569.1
Gallus gallus (chicken)	IFIT5	XP_421662.2
Danio rerio (zebrafish)	LOC100001239	NP_001177393.1
Xenopus tropicalis (tropical clawed frog)	LOC100487106	XP_002937238.2

Gene Ontology
Bibliography

Related articles in PubMed

Protective effect of a recombinant VHSV-G vaccine using poly(I:C) loaded nanoparticles as an adjuvant in zebrafish (Danio rerio) infection model.
Kavaliauskis A, Arnemo M, Speth M, Lagos L, Rishovd AL, Estepa A, Griffiths G, Gj?en T
Developmental and comparative immunology61248-57(2016 08)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Use of Poly(I:C) Stabilized with Chitosan As a Vaccine-Adjuvant Against Viral Hemorrhagic Septicemia Virus Infection in Zebrafish.
Kavaliauskis A, Arnemo M, Kim SH, Ulanova L, Speth M, Novoa B, Dios S, Evensen ?, Griffiths GW, Gj?en T
Zebrafish12(6)421-31(2015 Dec)

Interferon-induced genes of the expanded IFIT family show conserved antiviral activities in non-mammalian species.
Varela M, Diaz-Rosales P, Pereiro P, Forn-Cun? G, Costa MM, Dios S, Romero A, Figueras A, Novoa B
PloS one9(6)e100015(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ifit12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ifit12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa05255	NM_001190465.1 Latest version!	Danio rerio interferon-induced protein with tetratricopeptide repeats 12 (ifit12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ifit12 ( NM_001190465.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001177394.1 Danio rerio interferon-induced protein with tetratricopeptide repeats 12 (ifit12), mRNA.

Price & Availability↑

CloneID	ODa05255
Clone ID Related Accession (Same CDS sequence)	NM_001190465.1
Accession Version	NM_001190465.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-25
Organism	Danio rerio(zebrafish)
Product	interferon-induced protein with tetratricopeptide repeats 5-like
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX000534.11. On Jun 26, 2010 this sequence version replaced XM_690798.3. ##Evidence-Data-START## Transcript exon combination :: CO958541.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCAGAGA TGAGTTTAAT TCAGGCCAGT TTAAAAAAGA AACTGGATCA GCTGGAGTGC 
CACTTCACAT GGGACATTAC AAAAGATCAT CTAGACTTTA CGAACCTCCT CAACAGACTT 
GAGGAACAAA ACAAATTGGA TTTAGGGAAT GAGGAAGGAG CTGCACGAGC GCAGTGCTCA 
ATGGGATATC TAAAATTCCT TCTCAACTGC AAAGAAGAAG CTCTTACTCA CCTGTCGAGA 
TCTGAGGCAC TCATCAAAGA AAACTTTGCA GACAACAACG ACAAGGCTCT TATTGTGACC 
TATGGAAATT TTGCCTGGAT AAACTACCAC ATGGAGAACT ACACAGAGTG CGAAAGTTAC 
CTGGTTAAGC TTCAGAAGAT ACATGAAAGA TTTGCAACTG AGTCTTCAGC TGTTCCAGAG 
GTGCTTGGGG AGAAAGGGTG GACTTATCTT AAATTCTCTC GTAAATATTA TGACAAGGCA 
GTAGAAGTTT TTCAGAAAGC TGTGGAACTG GATCCAACAA ATAGTGAGTG GAACGCAGGT 
TATGCCATCG CTCTGTATCG CACTGAGAAT TTTAACCCAC CACCTTGTAC TGTGGAAACA 
CCTGCAATAA AACAGCTGAG ACAGGCCATT GACTTAAACC CAGATGATGA TGTTCTGAGA 
GTTATTCTAG GAATTAAACT TTTGAATTGC TCCAAAGGTT TAATGAACGA GTCCGAGCAA 
TTGGTTGAGA AAGCTTTGAA TCAGTCTCCA GAACATCCAC ATGTCATTAG ATATGTTGGA 
ATGTTCCTCA GGGATCAGGG ATCTGTTGAC AGGTCCATTG AAATTCTGAA GAAAGCCCTT 
CAAAGATCAC CAAAATCAGG CTTCATATGT CATCAATTAG CAATGTGCTA CAAGACCAAG 
AAAATAAACC TATGTAAAGA AAAACGGAGA AAAGTGGAGG TAGATGAGGC TCGCGATCAA 
AGCATATACT ACTTAGAAGA GGCAACTTCC TTAATACCAT CCTTTATCTC TGCAGTGTGT 
CAGCTCGGAC TTCAGTATGG AGACCGACGT GATCTGCTTA AAGCAGAAGA GCAGTTTGAC 
AAAGCATTTA AGTTAGTGAA AGAGAAAAAC GAGCATCTTC AATCTGTTCA TTTGTCTTAT 
GCTCAGTTCC AGCAGTACAG CAACAGACGT GAGGATTTAG CAATCGAGCA CTATGAGAAG 
TGTTTGACAA TGAATCCACA CACTCCTGAT GGAAATAGAA GTGCACAGAA ACTGTGCAAC 
ATTGCCAAGA AAAATCTCAA GTTAAATCCA AATGATTGGA AAGCCAATGA AATACTGGGC 
TTAGTTTATA ACTTGAAAGG TGAAATGTTT GAAAGGTATG GGAACACTCC AGACAACGAA 
GATGATTCAT TCCTCTGTAA TCTTCTTGAG GTTATGTCTT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177394.1
CDS	26..1450
Translation	MSEMSLIQASLKKKLDQLECHFTWDITKDHLDFTNLLNRLEEQNKLDLGNEEGAARAQCSMGYLKFLLNCKEEALTHLSRSEALIKENFADNNDKALIVTYGNFAWINYHMENYTECESYLVKLQKIHERFATESSAVPEVLGEKGWTYLKFSRKYYDKAVEVFQKAVELDPTNSEWNAGYAIALYRTENFNPPPCTVETPAIKQLRQAIDLNPDDDVLRVILGIKLLNCSKGLMNESEQLVEKALNQSPEHPHVIRYVGMFLRDQGSVDRSIEILKKALQRSPKSGFICHQLAMCYKTKKINLCKEKRRKVEVDEARDQSIYYLEEATSLIPSFISAVCQLGLQYGDRRDLLKAEEQFDKAFKLVKEKNEHLQSVHLSYAQFQQYSNRREDLAIEHYEKCLTMNPHTPDGNRSAQKLCNIAKKNLKLNPNDWKANEILGLVYNLKGEMFERYGNTPDNEDDSFLCNLLEVMSL

Target ORF information:

RefSeq Version	NM_001190465.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio interferon-induced protein with tetratricopeptide repeats 12 (ifit12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190465.1

ORF Insert Sequence:

ATGTCAGAGA TGAGTTTAAT TCAGGCCAGT TTAAAAAAGA AACTGGATCA GCTGGAGTGC 
CACTTCACAT GGGACATTAC AAAAGATCAT CTAGACTTTA CGAACCTCCT CAACAGACTT 
GAGGAACAAA ACAAATTGGA TTTAGGGAAT GAGGAAGGAG CTGCACGAGC GCAGTGCTCA 
ATGGGATATC TAAAATTCCT TCTCAACTGC AAAGAAGAAG CTCTTACTCA CCTGTCGAGA 
TCTGAGGCAC TCATCAAAGA AAACTTTGCA GACAACAACG ACAAGGCTCT TATTGTGACC 
TATGGAAATT TTGCCTGGAT AAACTACCAC ATGGAGAACT ACACAGAGTG CGAAAGTTAC 
CTGGTTAAGC TTCAGAAGAT ACATGAAAGA TTTGCAACTG AGTCTTCAGC TGTTCCAGAG 
GTGCTTGGGG AGAAAGGGTG GACTTATCTT AAATTCTCTC GTAAATATTA TGACAAGGCA 
GTAGAAGTTT TTCAGAAAGC TGTGGAACTG GATCCAACAA ATAGTGAGTG GAACGCAGGT 
TATGCCATCG CTCTGTATCG CACTGAGAAT TTTAACCCAC CACCTTGTAC TGTGGAAACA 
CCTGCAATAA AACAGCTGAG ACAGGCCATT GACTTAAACC CAGATGATGA TGTTCTGAGA 
GTTATTCTAG GAATTAAACT TTTGAATTGC TCCAAAGGTT TAATGAACGA GTCCGAGCAA 
TTGGTTGAGA AAGCTTTGAA TCAGTCTCCA GAACATCCAC ATGTCATTAG ATATGTTGGA 
ATGTTCCTCA GGGATCAGGG ATCTGTTGAC AGGTCCATTG AAATTCTGAA GAAAGCCCTT 
CAAAGATCAC CAAAATCAGG CTTCATATGT CATCAATTAG CAATGTGCTA CAAGACCAAG 
AAAATAAACC TATGTAAAGA AAAACGGAGA AAAGTGGAGG TAGATGAGGC TCGCGATCAA 
AGCATATACT ACTTAGAAGA GGCAACTTCC TTAATACCAT CCTTTATCTC TGCAGTGTGT 
CAGCTCGGAC TTCAGTATGG AGACCGACGT GATCTGCTTA AAGCAGAAGA GCAGTTTGAC 
AAAGCATTTA AGTTAGTGAA AGAGAAAAAC GAGCATCTTC AATCTGTTCA TTTGTCTTAT 
GCTCAGTTCC AGCAGTACAG CAACAGACGT GAGGATTTAG CAATCGAGCA CTATGAGAAG 
TGTTTGACAA TGAATCCACA CACTCCTGAT GGAAATAGAA GTGCACAGAA ACTGTGCAAC 
ATTGCCAAGA AAAATCTCAA GTTAAATCCA AATGATTGGA AAGCCAATGA AATACTGGGC 
TTAGTTTATA ACTTGAAAGG TGAAATGTTT GAAAGGTATG GGAACACTCC AGACAACGAA 
GATGATTCAT TCCTCTGTAA TCTTCTTGAG GTTATGTCTT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protective effect of a recombinant VHSV-G vaccine using poly(I:C) loaded nanoparticles as an adjuvant in zebrafish (Danio rerio) infection model.
Developmental and comparative immunology61248-57(2016 08)
Kavaliauskis A,Arnemo M,Speth M,Lagos L,Rishovd AL,Estepa A,Griffiths G,Gj?en T

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Use of Poly(I:C) Stabilized with Chitosan As a Vaccine-Adjuvant Against Viral Hemorrhagic Septicemia Virus Infection in Zebrafish.
Zebrafish12(6)421-31(2015 Dec)
Kavaliauskis A,Arnemo M,Kim SH,Ulanova L,Speth M,Novoa B,Dios S,Evensen ?,Griffiths GW,Gj?en T

Interferon-induced genes of the expanded IFIT family show conserved antiviral activities in non-mammalian species.
PloS one9(6)e100015(2014)
Varela M,Diaz-Rosales P,Pereiro P,Forn-Cun? G,Costa MM,Dios S,Romero A,Figueras A,Novoa B