SI:DKEY-192J17.1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SI:DKEY-192J17.1
Entrez Gene ID	566883
Full Name	si:dkey-192j17.1
General protein information	Preferred Names si:dkey-192j17.1 Names potassium voltage-gated channel subfamily B member 2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_690167.7	XP_695259.4	potassium voltage-gated channel subfamily B member 2
XM_690167.6	XP_695259.4	potassium voltage-gated channel subfamily B member 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	KCNB2	NP_004761.2
Danio rerio (zebrafish)	LOC566883	XP_695259.4
	KCNB2	XP_001083422.1
Rattus norvegicus (Norway rat)	Kcnb2	NP_446452.2
Gallus gallus (chicken)	KCNB2	XP_003640873.1
Xenopus tropicalis (tropical clawed frog)	kcnb2	XP_002937117.2
Pan troglodytes (chimpanzee)	KCNB2	XP_528164.2
Canis lupus familiaris (dog)	KCNB2	NP_001003247.1
Bos taurus (cattle)	KCNB2	NP_001019734.1
Mus musculus (house mouse)	Kcnb2	NP_001091998.1

Gene Ontology

The following SI:DKEY-192J17.1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI:DKEY-192J17.1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa28444	XM_690167.7 Latest version!	Danio rerio si:dkey-192j17.1 (si:dkey-192j17.1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa28444	XM_690167.6	Danio rerio si:dkey-192j17.1 (si:dkey-192j17.1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SI:DKEY-192J17.1 ( XM_690167.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_695259.4 Danio rerio si:dkey-192j17.1 (si:dkey-192j17.1), mRNA.

Price & Availability↑

CloneID	ODa28444
Clone ID Related Accession (Same CDS sequence)	XM_690167.7 , XM_690167.6
Accession Version	XM_690167.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	potassium voltage-gated channel subfamily B member 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_690167.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGA AGGCTGGATT GGGGGTCCCA AAACCTGGAG GCAGATCTGT GTCTTCACTT 
CCACCCGAGC CAATCGAGAT CATCCGGACC AAAGCCCGTT CCAGGAGGGT CCGAATTAAT 
GTCGGAGGCC TCAACCATGA GGTCCTATGG CGGACATTAG ACCGACTTCC CAGAACCAGA 
CTGGGCAAGC TGAGAGATTG TAACACCCAT GACTCTCTAA TGGAGGTCTG CGACGACTAC 
TGCCTGAATG AGAACGAATA CTTCTTTGAC AGACACCCCG GGGCGTTCAC GTCCATCTTG 
AATTTTTATC GAACTGGAAA GCTCCATATG ATGGAAGAAA TGTGCGCTCT GTCTTTCGGG 
CAGGAGCTTG ATTACTGGGG CATTGATGAG ATCTACCTGG AGTCGTGTTG CCAGGCTCGT 
TACCATCAGA AGAAAGAGCA GATGAATGAG GAATTGAGGA GAGAGGCAGA GACTCTGAGA 
GAGAGGGAGG GAGAAGGGGA ATTTGATAAC ACCTGCTGCC CAGAAAAGAG GAAAAAACTT 
TGGGAGCTTT TGGAGAAGCC AAGTTCTTCG GTTGCTGCTA AGATACTGGC CATCATATCC 
ATTCTGTTCA TCGTTCTTTC AACAGTTGCA CTGTCCCTGA ACACGTTGCC CGAACTCCAA 
GTGACAGATG AATTCGGTCA AGCCAATGAC AATCCTCAAC TCGCTCACGT TGAAGCCGTG 
TGTATCGCAT GGTTTACAAT GGAGTATCTC CTGCGCTTCC TGTCCTCACC AAATAAGTGG 
AAATTCTTCA AAGGGCCACT AAATGTCATT GACTTGTTAG CTATACTACC CTACTATGTC 
ACAATTTTTC TCACTGAGTC AAACAAAAGT GTGCTGCAGT TCCAAAACGT GAGGCGGGTG 
GTGCAGATAT TCCGCATCAT GAGAATACTG AGGATTCTGA AGTTGGCAAG ACATTCAACA 
GGACTACAGT CTCTAGGATT CACCCTCAGA CGCAGCTATA ATGAGCTGGG CTTACTCATA 
TTGTTCTTAG CCATGGGAAT TATGATATTC TCAAGTTTGG TCTTCTTTGC AGAAAAGGAT 
GAGGATGCAA CCAAATTTAC AAGCATCCCA GCTTCATTTT GGTGGGCTAC TATCACCATG 
ACGACAGTAG GCTATGGAGA TATATACCCT CAGACTCTGC TTGGTAAAAT AGTCGGTGGA 
CTTTGCTGCA TTGCTGGAGT GCTGGTTATT GCCTTACCCA TCCCTATTAT TGTGAATAAC 
TTCTCCGAAT TCTACAAGGA GCAGAAACGA CAGGAGAAAG CCATCAAGAG GAGAGAGGCC 
TTAGAACGAG CAAAGAGAAA TGGCAGCATC GTCTCAATGA ACCTGAAAGA TGCCTTTGCA 
CGAAGTATGG AGCTAATGGA CGTCACTGTA GAAAAAAGTG ATGACAAACG TCTAACAGAC 
AACCACCTTT CTCCGAGTCG GTGGGGAATT CCACAGCGCA CATCCTCAGA CACCAGCCTC 
AGATCCTTTG ACAAAAAGTT TCCCAACAGC GGGTCTCAGG AATATGTCCA CATGCCGAGT 
CCACAGCATC TCAATGCTCA GAATCTGGAG GAAATGTACA ACCAGATGAC AAAGACACAG 
TCCTTCTCAG ATCTCAGCAC AAGCAACAGC ACACAACCTG CGGTGGTGTC CAAACATGAG 
GTAGAGTTGG AGATGAAGGA AGTCCCGACA GCCGTCAAAC AGCAACCCAA GGAAAGAACC 
ATCACGGATG TCAGAAGCAT ATCAAGTGTT GATAGCTTTG CAAGCTGCAC TGCTGAATTT 
GGACAGGGTG AGAAAGCAAC CACCCCTTCT CATACAGCAG AGCAGTTTCA GCAAAGGCTA 
CCAAAGGTTT CTCCGCAGCT TCACCTTGGT CAGCTGACAC CCTCAGACTT AAGTCCTGAG 
GAAAACTCTG GCATTGTCAA TCGAGACACT GATGACCTCA ATGAGTTTGT GGAGAACATC 
AGAAGCTCCA TTCGTGGCAC AAATCCAATC AAAGCTAAAG GCCTCAAAGT CAACTTCATA 
GAAACGCCAG AGGATGCTCC TCTGACACCA CCAAACACTT CATCTCCACA AGACATCAAC 
AACAGTCTAC TGGAAGATGA CTCCCCAGAG GGAGAGTGCT CTCCTCTCTG CTCAGATTCA 
GAGACGGTGG GTGGTAACTC AATGGAAGGC TTGGTTCTAT CTCCGAAGAA GCTACCGGGC 
AGATGCCATC CTGGGGAGAC AGTGCTGCTT TTGGGGAATC TTGAGGAACA GGAGTATCTC 
GATGAACAAA ACCAAATGGA AGTAGCTTCC GCAGCCATCT CCTCGCCCAA ACTAGCAGCA 
CAGAACTGCT CGCAGAATAT AATCGGTGGT AACAGCGAGG AAAAGCCAGA CTCTAACCAA 
AGTCAACACA AGGTAGAGAA CCACACGTTT ACACCGGAGA TCCATCTAAT GCCAGGGGAT 
GGTAATCACT CGCCATCTTG CAAAACCAGT ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_695259.4
CDS	562..3057
Translation	MAEKAGLGVPKPGGRSVSSLPPEPIEIIRTKARSRRVRINVGGLNHEVLWRTLDRLPRTRLGKLRDCNTHDSLMEVCDDYCLNENEYFFDRHPGAFTSILNFYRTGKLHMMEEMCALSFGQELDYWGIDEIYLESCCQARYHQKKEQMNEELRREAETLREREGEGEFDNTCCPEKRKKLWELLEKPSSSVAAKILAIISILFIVLSTVALSLNTLPELQVTDEFGQANDNPQLAHVEAVCIAWFTMEYLLRFLSSPNKWKFFKGPLNVIDLLAILPYYVTIFLTESNKSVLQFQNVRRVVQIFRIMRILRILKLARHSTGLQSLGFTLRRSYNELGLLILFLAMGIMIFSSLVFFAEKDEDATKFTSIPASFWWATITMTTVGYGDIYPQTLLGKIVGGLCCIAGVLVIALPIPIIVNNFSEFYKEQKRQEKAIKRREALERAKRNGSIVSMNLKDAFARSMELMDVTVEKSDDKRLTDNHLSPSRWGIPQRTSSDTSLRSFDKKFPNSGSQEYVHMPSPQHLNAQNLEEMYNQMTKTQSFSDLSTSNSTQPAVVSKHEVELEMKEVPTAVKQQPKERTITDVRSISSVDSFASCTAEFGQGEKATTPSHTAEQFQQRLPKVSPQLHLGQLTPSDLSPEENSGIVNRDTDDLNEFVENIRSSIRGTNPIKAKGLKVNFIETPEDAPLTPPNTSSPQDINNSLLEDDSPEGECSPLCSDSETVGGNSMEGLVLSPKKLPGRCHPGETVLLLGNLEEQEYLDEQNQMEVASAAISSPKLAAQNCSQNIIGGNSEEKPDSNQSQHKVENHTFTPEIHLMPGDGNHSPSCKTSM

Target ORF information:

RefSeq Version	XM_690167.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-192j17.1 (si:dkey-192j17.1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_690167.7

ORF Insert Sequence:

ATGGCAGAGA AGGCTGGATT GGGGGTCCCA AAACCTGGAG GCAGATCTGT GTCTTCACTT 
CCACCCGAGC CAATCGAGAT CATCCGGACC AAAGCCCGTT CCAGGAGGGT CCGAATTAAT 
GTCGGAGGCC TCAACCATGA GGTCCTATGG CGGACATTAG ACCGACTTCC CAGAACCAGA 
CTGGGCAAGC TGAGAGATTG TAACACCCAT GACTCTCTAA TGGAGGTCTG CGACGACTAC 
TGCCTGAATG AGAACGAATA CTTCTTTGAC AGACACCCCG GGGCGTTCAC GTCCATCTTG 
AATTTTTATC GAACTGGAAA GCTCCATATG ATGGAAGAAA TGTGCGCTCT GTCTTTCGGG 
CAGGAGCTTG ATTACTGGGG CATTGATGAG ATCTACCTGG AGTCGTGTTG CCAGGCTCGT 
TACCATCAGA AGAAAGAGCA GATGAATGAG GAATTGAGGA GAGAGGCAGA GACTCTGAGA 
GAGAGGGAGG GAGAAGGGGA ATTTGATAAC ACCTGCTGCC CAGAAAAGAG GAAAAAACTT 
TGGGAGCTTT TGGAGAAGCC AAGTTCTTCG GTTGCTGCTA AGATACTGGC CATCATATCC 
ATTCTGTTCA TCGTTCTTTC AACAGTTGCA CTGTCCCTGA ACACGTTGCC CGAACTCCAA 
GTGACAGATG AATTCGGTCA AGCCAATGAC AATCCTCAAC TCGCTCACGT TGAAGCCGTG 
TGTATCGCAT GGTTTACAAT GGAGTATCTC CTGCGCTTCC TGTCCTCACC AAATAAGTGG 
AAATTCTTCA AAGGGCCACT AAATGTCATT GACTTGTTAG CTATACTACC CTACTATGTC 
ACAATTTTTC TCACTGAGTC AAACAAAAGT GTGCTGCAGT TCCAAAACGT GAGGCGGGTG 
GTGCAGATAT TCCGCATCAT GAGAATACTG AGGATTCTGA AGTTGGCAAG ACATTCAACA 
GGACTACAGT CTCTAGGATT CACCCTCAGA CGCAGCTATA ATGAGCTGGG CTTACTCATA 
TTGTTCTTAG CCATGGGAAT TATGATATTC TCAAGTTTGG TCTTCTTTGC AGAAAAGGAT 
GAGGATGCAA CCAAATTTAC AAGCATCCCA GCTTCATTTT GGTGGGCTAC TATCACCATG 
ACGACAGTAG GCTATGGAGA TATATACCCT CAGACTCTGC TTGGTAAAAT AGTCGGTGGA 
CTTTGCTGCA TTGCTGGAGT GCTGGTTATT GCCTTACCCA TCCCTATTAT TGTGAATAAC 
TTCTCCGAAT TCTACAAGGA GCAGAAACGA CAGGAGAAAG CCATCAAGAG GAGAGAGGCC 
TTAGAACGAG CAAAGAGAAA TGGCAGCATC GTCTCAATGA ACCTGAAAGA TGCCTTTGCA 
CGAAGTATGG AGCTAATGGA CGTCACTGTA GAAAAAAGTG ATGACAAACG TCTAACAGAC 
AACCACCTTT CTCCGAGTCG GTGGGGAATT CCACAGCGCA CATCCTCAGA CACCAGCCTC 
AGATCCTTTG ACAAAAAGTT TCCCAACAGC GGGTCTCAGG AATATGTCCA CATGCCGAGT 
CCACAGCATC TCAATGCTCA GAATCTGGAG GAAATGTACA ACCAGATGAC AAAGACACAG 
TCCTTCTCAG ATCTCAGCAC AAGCAACAGC ACACAACCTG CGGTGGTGTC CAAACATGAG 
GTAGAGTTGG AGATGAAGGA AGTCCCGACA GCCGTCAAAC AGCAACCCAA GGAAAGAACC 
ATCACGGATG TCAGAAGCAT ATCAAGTGTT GATAGCTTTG CAAGCTGCAC TGCTGAATTT 
GGACAGGGTG AGAAAGCAAC CACCCCTTCT CATACAGCAG AGCAGTTTCA GCAAAGGCTA 
CCAAAGGTTT CTCCGCAGCT TCACCTTGGT CAGCTGACAC CCTCAGACTT AAGTCCTGAG 
GAAAACTCTG GCATTGTCAA TCGAGACACT GATGACCTCA ATGAGTTTGT GGAGAACATC 
AGAAGCTCCA TTCGTGGCAC AAATCCAATC AAAGCTAAAG GCCTCAAAGT CAACTTCATA 
GAAACGCCAG AGGATGCTCC TCTGACACCA CCAAACACTT CATCTCCACA AGACATCAAC 
AACAGTCTAC TGGAAGATGA CTCCCCAGAG GGAGAGTGCT CTCCTCTCTG CTCAGATTCA 
GAGACGGTGG GTGGTAACTC AATGGAAGGC TTGGTTCTAT CTCCGAAGAA GCTACCGGGC 
AGATGCCATC CTGGGGAGAC AGTGCTGCTT TTGGGGAATC TTGAGGAACA GGAGTATCTC 
GATGAACAAA ACCAAATGGA AGTAGCTTCC GCAGCCATCT CCTCGCCCAA ACTAGCAGCA 
CAGAACTGCT CGCAGAATAT AATCGGTGGT AACAGCGAGG AAAAGCCAGA CTCTAACCAA 
AGTCAACACA AGGTAGAGAA CCACACGTTT ACACCGGAGA TCCATCTAAT GCCAGGGGAT 
GGTAATCACT CGCCATCTTG CAAAACCAGT ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEY-192J17.1 ( XM_690167.6 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_695259.4 Danio rerio si:dkey-192j17.1 (si:dkey-192j17.1), mRNA.

Price & Availability↑

CloneID	ODa28444
Clone ID Related Accession (Same CDS sequence)	XM_690167.7 , XM_690167.6
Accession Version	XM_690167.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	potassium voltage-gated channel subfamily B member 2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_690167.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGA AGGCTGGATT GGGGGTCCCA AAACCTGGAG GCAGATCTGT GTCTTCACTT 
CCACCCGAGC CAATCGAGAT CATCCGGACC AAAGCCCGTT CCAGGAGGGT CCGAATTAAT 
GTCGGAGGCC TCAACCATGA GGTCCTATGG CGGACATTAG ACCGACTTCC CAGAACCAGA 
CTGGGCAAGC TGAGAGATTG TAACACCCAT GACTCTCTAA TGGAGGTCTG CGACGACTAC 
TGCCTGAATG AGAACGAATA CTTCTTTGAC AGACACCCCG GGGCGTTCAC GTCCATCTTG 
AATTTTTATC GAACTGGAAA GCTCCATATG ATGGAAGAAA TGTGCGCTCT GTCTTTCGGG 
CAGGAGCTTG ATTACTGGGG CATTGATGAG ATCTACCTGG AGTCGTGTTG CCAGGCTCGT 
TACCATCAGA AGAAAGAGCA GATGAATGAG GAATTGAGGA GAGAGGCAGA GACTCTGAGA 
GAGAGGGAGG GAGAAGGGGA ATTTGATAAC ACCTGCTGCC CAGAAAAGAG GAAAAAACTT 
TGGGAGCTTT TGGAGAAGCC AAGTTCTTCG GTTGCTGCTA AGATACTGGC CATCATATCC 
ATTCTGTTCA TCGTTCTTTC AACAGTTGCA CTGTCCCTGA ACACGTTGCC CGAACTCCAA 
GTGACAGATG AATTCGGTCA AGCCAATGAC AATCCTCAAC TCGCTCACGT TGAAGCCGTG 
TGTATCGCAT GGTTTACAAT GGAGTATCTC CTGCGCTTCC TGTCCTCACC AAATAAGTGG 
AAATTCTTCA AAGGGCCACT AAATGTCATT GACTTGTTAG CTATACTACC CTACTATGTC 
ACAATTTTTC TCACTGAGTC AAACAAAAGT GTGCTGCAGT TCCAAAACGT GAGGCGGGTG 
GTGCAGATAT TCCGCATCAT GAGAATACTG AGGATTCTGA AGTTGGCAAG ACATTCAACA 
GGACTACAGT CTCTAGGATT CACCCTCAGA CGCAGCTATA ATGAGCTGGG CTTACTCATA 
TTGTTCTTAG CCATGGGAAT TATGATATTC TCAAGTTTGG TCTTCTTTGC AGAAAAGGAT 
GAGGATGCAA CCAAATTTAC AAGCATCCCA GCTTCATTTT GGTGGGCTAC TATCACCATG 
ACGACAGTAG GCTATGGAGA TATATACCCT CAGACTCTGC TTGGTAAAAT AGTCGGTGGA 
CTTTGCTGCA TTGCTGGAGT GCTGGTTATT GCCTTACCCA TCCCTATTAT TGTGAATAAC 
TTCTCCGAAT TCTACAAGGA GCAGAAACGA CAGGAGAAAG CCATCAAGAG GAGAGAGGCC 
TTAGAACGAG CAAAGAGAAA TGGCAGCATC GTCTCAATGA ACCTGAAAGA TGCCTTTGCA 
CGAAGTATGG AGCTAATGGA CGTCACTGTA GAAAAAAGTG ATGACAAACG TCTAACAGAC 
AACCACCTTT CTCCGAGTCG GTGGGGAATT CCACAGCGCA CATCCTCAGA CACCAGCCTC 
AGATCCTTTG ACAAAAAGTT TCCCAACAGC GGGTCTCAGG AATATGTCCA CATGCCGAGT 
CCACAGCATC TCAATGCTCA GAATCTGGAG GAAATGTACA ACCAGATGAC AAAGACACAG 
TCCTTCTCAG ATCTCAGCAC AAGCAACAGC ACACAACCTG CGGTGGTGTC CAAACATGAG 
GTAGAGTTGG AGATGAAGGA AGTCCCGACA GCCGTCAAAC AGCAACCCAA GGAAAGAACC 
ATCACGGATG TCAGAAGCAT ATCAAGTGTT GATAGCTTTG CAAGCTGCAC TGCTGAATTT 
GGACAGGGTG AGAAAGCAAC CACCCCTTCT CATACAGCAG AGCAGTTTCA GCAAAGGCTA 
CCAAAGGTTT CTCCGCAGCT TCACCTTGGT CAGCTGACAC CCTCAGACTT AAGTCCTGAG 
GAAAACTCTG GCATTGTCAA TCGAGACACT GATGACCTCA ATGAGTTTGT GGAGAACATC 
AGAAGCTCCA TTCGTGGCAC AAATCCAATC AAAGCTAAAG GCCTCAAAGT CAACTTCATA 
GAAACGCCAG AGGATGCTCC TCTGACACCA CCAAACACTT CATCTCCACA AGACATCAAC 
AACAGTCTAC TGGAAGATGA CTCCCCAGAG GGAGAGTGCT CTCCTCTCTG CTCAGATTCA 
GAGACGGTGG GTGGTAACTC AATGGAAGGC TTGGTTCTAT CTCCGAAGAA GCTACCGGGC 
AGATGCCATC CTGGGGAGAC AGTGCTGCTT TTGGGGAATC TTGAGGAACA GGAGTATCTC 
GATGAACAAA ACCAAATGGA AGTAGCTTCC GCAGCCATCT CCTCGCCCAA ACTAGCAGCA 
CAGAACTGCT CGCAGAATAT AATCGGTGGT AACAGCGAGG AAAAGCCAGA CTCTAACCAA 
AGTCAACACA AGGTAGAGAA CCACACGTTT ACACCGGAGA TCCATCTAAT GCCAGGGGAT 
GGTAATCACT CGCCATCTTG CAAAACCAGT ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_695259.4
CDS	556..3051
Translation	MAEKAGLGVPKPGGRSVSSLPPEPIEIIRTKARSRRVRINVGGLNHEVLWRTLDRLPRTRLGKLRDCNTHDSLMEVCDDYCLNENEYFFDRHPGAFTSILNFYRTGKLHMMEEMCALSFGQELDYWGIDEIYLESCCQARYHQKKEQMNEELRREAETLREREGEGEFDNTCCPEKRKKLWELLEKPSSSVAAKILAIISILFIVLSTVALSLNTLPELQVTDEFGQANDNPQLAHVEAVCIAWFTMEYLLRFLSSPNKWKFFKGPLNVIDLLAILPYYVTIFLTESNKSVLQFQNVRRVVQIFRIMRILRILKLARHSTGLQSLGFTLRRSYNELGLLILFLAMGIMIFSSLVFFAEKDEDATKFTSIPASFWWATITMTTVGYGDIYPQTLLGKIVGGLCCIAGVLVIALPIPIIVNNFSEFYKEQKRQEKAIKRREALERAKRNGSIVSMNLKDAFARSMELMDVTVEKSDDKRLTDNHLSPSRWGIPQRTSSDTSLRSFDKKFPNSGSQEYVHMPSPQHLNAQNLEEMYNQMTKTQSFSDLSTSNSTQPAVVSKHEVELEMKEVPTAVKQQPKERTITDVRSISSVDSFASCTAEFGQGEKATTPSHTAEQFQQRLPKVSPQLHLGQLTPSDLSPEENSGIVNRDTDDLNEFVENIRSSIRGTNPIKAKGLKVNFIETPEDAPLTPPNTSSPQDINNSLLEDDSPEGECSPLCSDSETVGGNSMEGLVLSPKKLPGRCHPGETVLLLGNLEEQEYLDEQNQMEVASAAISSPKLAAQNCSQNIIGGNSEEKPDSNQSQHKVENHTFTPEIHLMPGDGNHSPSCKTSM

Target ORF information:

RefSeq Version	XM_690167.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:dkey-192j17.1 (si:dkey-192j17.1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_690167.6

ORF Insert Sequence:

ATGGCAGAGA AGGCTGGATT GGGGGTCCCA AAACCTGGAG GCAGATCTGT GTCTTCACTT 
CCACCCGAGC CAATCGAGAT CATCCGGACC AAAGCCCGTT CCAGGAGGGT CCGAATTAAT 
GTCGGAGGCC TCAACCATGA GGTCCTATGG CGGACATTAG ACCGACTTCC CAGAACCAGA 
CTGGGCAAGC TGAGAGATTG TAACACCCAT GACTCTCTAA TGGAGGTCTG CGACGACTAC 
TGCCTGAATG AGAACGAATA CTTCTTTGAC AGACACCCCG GGGCGTTCAC GTCCATCTTG 
AATTTTTATC GAACTGGAAA GCTCCATATG ATGGAAGAAA TGTGCGCTCT GTCTTTCGGG 
CAGGAGCTTG ATTACTGGGG CATTGATGAG ATCTACCTGG AGTCGTGTTG CCAGGCTCGT 
TACCATCAGA AGAAAGAGCA GATGAATGAG GAATTGAGGA GAGAGGCAGA GACTCTGAGA 
GAGAGGGAGG GAGAAGGGGA ATTTGATAAC ACCTGCTGCC CAGAAAAGAG GAAAAAACTT 
TGGGAGCTTT TGGAGAAGCC AAGTTCTTCG GTTGCTGCTA AGATACTGGC CATCATATCC 
ATTCTGTTCA TCGTTCTTTC AACAGTTGCA CTGTCCCTGA ACACGTTGCC CGAACTCCAA 
GTGACAGATG AATTCGGTCA AGCCAATGAC AATCCTCAAC TCGCTCACGT TGAAGCCGTG 
TGTATCGCAT GGTTTACAAT GGAGTATCTC CTGCGCTTCC TGTCCTCACC AAATAAGTGG 
AAATTCTTCA AAGGGCCACT AAATGTCATT GACTTGTTAG CTATACTACC CTACTATGTC 
ACAATTTTTC TCACTGAGTC AAACAAAAGT GTGCTGCAGT TCCAAAACGT GAGGCGGGTG 
GTGCAGATAT TCCGCATCAT GAGAATACTG AGGATTCTGA AGTTGGCAAG ACATTCAACA 
GGACTACAGT CTCTAGGATT CACCCTCAGA CGCAGCTATA ATGAGCTGGG CTTACTCATA 
TTGTTCTTAG CCATGGGAAT TATGATATTC TCAAGTTTGG TCTTCTTTGC AGAAAAGGAT 
GAGGATGCAA CCAAATTTAC AAGCATCCCA GCTTCATTTT GGTGGGCTAC TATCACCATG 
ACGACAGTAG GCTATGGAGA TATATACCCT CAGACTCTGC TTGGTAAAAT AGTCGGTGGA 
CTTTGCTGCA TTGCTGGAGT GCTGGTTATT GCCTTACCCA TCCCTATTAT TGTGAATAAC 
TTCTCCGAAT TCTACAAGGA GCAGAAACGA CAGGAGAAAG CCATCAAGAG GAGAGAGGCC 
TTAGAACGAG CAAAGAGAAA TGGCAGCATC GTCTCAATGA ACCTGAAAGA TGCCTTTGCA 
CGAAGTATGG AGCTAATGGA CGTCACTGTA GAAAAAAGTG ATGACAAACG TCTAACAGAC 
AACCACCTTT CTCCGAGTCG GTGGGGAATT CCACAGCGCA CATCCTCAGA CACCAGCCTC 
AGATCCTTTG ACAAAAAGTT TCCCAACAGC GGGTCTCAGG AATATGTCCA CATGCCGAGT 
CCACAGCATC TCAATGCTCA GAATCTGGAG GAAATGTACA ACCAGATGAC AAAGACACAG 
TCCTTCTCAG ATCTCAGCAC AAGCAACAGC ACACAACCTG CGGTGGTGTC CAAACATGAG 
GTAGAGTTGG AGATGAAGGA AGTCCCGACA GCCGTCAAAC AGCAACCCAA GGAAAGAACC 
ATCACGGATG TCAGAAGCAT ATCAAGTGTT GATAGCTTTG CAAGCTGCAC TGCTGAATTT 
GGACAGGGTG AGAAAGCAAC CACCCCTTCT CATACAGCAG AGCAGTTTCA GCAAAGGCTA 
CCAAAGGTTT CTCCGCAGCT TCACCTTGGT CAGCTGACAC CCTCAGACTT AAGTCCTGAG 
GAAAACTCTG GCATTGTCAA TCGAGACACT GATGACCTCA ATGAGTTTGT GGAGAACATC 
AGAAGCTCCA TTCGTGGCAC AAATCCAATC AAAGCTAAAG GCCTCAAAGT CAACTTCATA 
GAAACGCCAG AGGATGCTCC TCTGACACCA CCAAACACTT CATCTCCACA AGACATCAAC 
AACAGTCTAC TGGAAGATGA CTCCCCAGAG GGAGAGTGCT CTCCTCTCTG CTCAGATTCA 
GAGACGGTGG GTGGTAACTC AATGGAAGGC TTGGTTCTAT CTCCGAAGAA GCTACCGGGC 
AGATGCCATC CTGGGGAGAC AGTGCTGCTT TTGGGGAATC TTGAGGAACA GGAGTATCTC 
GATGAACAAA ACCAAATGGA AGTAGCTTCC GCAGCCATCT CCTCGCCCAA ACTAGCAGCA 
CAGAACTGCT CGCAGAATAT AATCGGTGGT AACAGCGAGG AAAAGCCAGA CTCTAACCAA 
AGTCAACACA AGGTAGAGAA CCACACGTTT ACACCGGAGA TCCATCTAAT GCCAGGGGAT 
GGTAATCACT CGCCATCTTG CAAAACCAGT ATGTGA