E2F8 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	E2F8
Entrez Gene ID	565952
Full Name	E2F transcription factor 8
Synonyms	im:7149356,si:ch211-215h6.2,wu:fc06b06
General protein information	Preferred Names E2F transcription factor 8 Names transcription factor E2F8 E2F family member 8
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001347691.1	NP_001334620.1	transcription factor E2F8
XM_689219.8	XP_694311.6	transcription factor E2F8

Pathways from BioSystems

Homologies

Homo sapiens (human)	E2F8	NP_001243300.1
	E2F8	XP_001095088.1
Bos taurus (cattle)	E2F8	NP_001178163.1
Pan troglodytes (chimpanzee)	E2F8	XP_508325.2
Gallus gallus (chicken)	E2F8	XP_420910.4
Mus musculus (house mouse)	E2f8	NP_001013386.2
Canis lupus familiaris (dog)	E2F8	XP_534087.2
Rattus norvegicus (Norway rat)	E2f8	XP_006229325.1
Danio rerio (zebrafish)	e2f8	XP_005168886.1
Xenopus tropicalis (tropical clawed frog)	e2f8	XP_002942881.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide analysis reveals NRP1 as a direct HIF1?-E2F7 target in the regulation of motorneuron guidance in vivo.
de Bruin A, A Cornelissen PW, Kirchmaier BC, Mokry M, Iich E, Nirmala E, Liang KH, D V?gh AM, Scholman KT, Groot Koerkamp MJ, Holstege FC, Cuppen E, Schulte-Merker S, Bakker WJ
Nucleic acids research44(8)3549-66(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

E2F8 promotes hepatic steatosis through FABP3 expression in diet-induced obesity in zebrafish.
Shimada Y, Kuninaga S, Ariyoshi M, Zhang B, Shiina Y, Takahashi Y, Umemoto N, Nishimura Y, Enari H, Tanaka T
Nutrition & metabolism1217(2015)

Evolution of the vertebrate Pax4/6 class of genes with focus on its novel member, the Pax10 gene.
Feiner N, Meyer A, Kuraku S
Genome biology and evolution6(7)1635-51(2014 Jun)

Atypical E2fs control lymphangiogenesis through transcriptional regulation of Ccbe1 and Flt4.
Weijts BG, van Impel A, Schulte-Merker S, de Bruin A
PloS one8(9)e73693(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following E2F8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the E2F8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa12735	NM_001347691.1 Latest version!	Danio rerio E2F transcription factor 8 (e2f8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa12735	XM_689219.8 Latest version!	Danio rerio E2F transcription factor 8 (e2f8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

E2F8 ( NM_001347691.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001334620.1 Danio rerio E2F transcription factor 8 (e2f8), mRNA.

Price & Availability↑

CloneID	ODa12735
Clone ID Related Accession (Same CDS sequence)	NM_001347691.1 , XM_689219.8
Accession Version	NM_001347691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	transcription factor E2F8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX890598.10 and CU041375.7. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01019896.1, GFIL01009118.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAGTA CATTATCAGA AGGTCAAACA TTAATCAAGA AATCTTTAAG TCCATCAAAG 
GCAACATCTA CAAATAACAA GGGGCATGTT TTTGTGGAGC CACAAACGCC ACTAAAAAAC 
TCAAATAAAG CTTCAACATC TGAAGCTGCT CTACCGGAGA CCCTTAAAAT CATGGGGCCC 
CTGACAACGC CTACTAAGGT CCTAGATGCA CCTTCAAGCG ACCCTTGGAC ACCCACTTCT 
AACCTAAAAA TGCTTATCAG TGCAGCCAGT CCTGAGATCC GCAACCGCGA GAAAGAACGT 
GCCGTGGACA GCAGCGAATC CGAGAACTCA CAGGAGACCG AGCAAGGGGA AGAAGTAGAA 
AAGCTCCATA TAAGCCGTAA AGACAAAAGC CTTGGTTTGT TGTGTTACAA GTTCCTTGCA 
AGGTACCCAA ACTACCCCAA CCCTGCTCTA AACAACGGTA TCAGCCTTGA TGATGTGGCA 
GCAGAACTGC ACGTGGAAAG ACGTCGCATC TACGACATCA TGAATGTCCT TGAAAGCCTT 
AACATGGTCA GCCGGCTGGC AAAGAATCGC TACACTTGGC ATGGTCGAGT AAAACTTGCA 
CAGACGCTTG CTGTGCTGAA ACGGGCCGGA AAAGAGAACC GCTATGAACA GCTTATGCAG 
CAGATCCGAC AAAGGAGTCA AGAGCGAGAA GAGAGAGAGT TCGATTTGGA TGGAGAGGAG 
AAGGAGAATG AAGAAATGTC CAGTTTTGAA GTTGACGGAG ACTCTGGGCT GGCGGATCTT 
CCAGGAGCGG ATTCTAAAGC AGCTTCTGCG AACAGTCGGA AAGACAAATC TCTGCGAGTG 
ATGAGTCAGA AGTTTGTCAT GTTGTTCCTG GTGTCCAGTC CTCCTGTGGT TAGTCTGGAT 
GTAGCTGCCA AAATCCTAAT CGGTGAAGAT CATGTGGTGG ACCAGGACAA AAACAAGTTC 
AAAACCAAGA TCCGTAGACT TTATGACATT GCGAATGTTT TGAGCAGCCT AGAGCTGATT 
AAGAAAGTGC ACGTGACTGA AGATAAAGGA AGAAAGCCTG CTTTCAAATG GACTGGGCCA 
GAAGACATAC CATCTCCAAA GGATCTGGAG ATCTCCACTA CATCCTCAGC ACCAAAGCCT 
CTCGAGTCTC GTTCCTCTGT TGAAAACTGT GCTAAAAATC TGTTTTCCTC CCCTGGAACA 
AAGCGAGGTT TTACCCGCCA TCATTCTCTA GTCAAGCTGG TTAAGAGCAT TCAGGATGAC 
CGCAGGAAAA TCAACTCTGC GCCTTCTAGT CCAATTAAAA TGACAGGTGA TTCTGCAGAC 
AGTGATTTCT ATACTACCAA AATGGCCCAT CTGGCAGCTA TCTGCAAAAA GCACCTCGAT 
GAACAGTCAG CAGACGGTCG ACCTAACAAT GCGGTGACAG ACTCTTCACA GTCATCTAAA 
CAGCCTGAAT CCACATCTGC TAGTAACCAT GGGCCTCCAG GGATGCAGAT CCCAGTTCTG 
CCAGCAGGAG CAATTTCATA CCTTCCCACA AAATGTTCCC CGATCATCCC ACTTCTGATA 
CCTCAGCACC AGACTGGTGG GCCGTATGCC GTTTACATGC ACCCAACGTC TCTCAGACCA 
CAGCCCACCA GCCTGGCTGT CCGTTCAATG ACATTCGAGA GTCCAGTTGG CGCAAATGCA 
AAGACATCTC CGGCTACTTT GACTAGCAAT AACCAGACAA ACCAGTCGTC ATCGTATGGT 
AAAGAGCAGA CAAGTCCTGT GAACCTTAAA CGGGCAAGTG GAGAAAAGAG CTCCGTGGGA 
AGTCCATCCA AAATGCAACG GACAGAGCCA AAGAGCGTTT CTCCAAAGCT GTGTGAGATT 
CTACAAGCAC GTCTGAAGGC CCGTAGAGGA GCTTTGACCT CCAACCGCCC TTCCGCAAGA 
GCTCTTCATC TGGAGTTCTC CAAACCCTCT GAAAGCCAGC CAACGGTACA AACCGGCACA 
GCATCTCTGG AGCACAGCCT GGAGACCTTT CTAGAGAAGG AGGAGAAGAG CCAGACCTCA 
GACAATGAGG CAGGACTGAC CCCTGTAAGA CAACCACATT CACAGCCCCA GAAGCTCAGC 
GCTCCCTTTC AGGACATGGT GCTGCCATCT GGACCCATAC ACACAGAGAC ATTGATCCCC 
GCGGGTTATT TGATTCCCAT CTCTCAGCAG TCCATTGTGA ACTTCAGAGA ACCGCAGTGC 
TCCAATGAAA GCTCCAAAGC CTCCACACCA ACATACAACA TCTATCACAC ACCAACAGCA 
GGTTCCAGAC CAGCTTTTCC TCAAGAAGTC ACCCCGACCC GACTCCCTCT TCACCGGATA 
CCGCCCATCT CCCCCTTCCC TTCTCACGGA CATCGCCTCC ACAGCCCAAG TCCTGCTATT 
CTCAACTTCA CCCTGCAGAA TCTGGGCCTC ATTCCTGGCA GTGTTACACC AAACCCACAC 
ACTCCTGAGC AGAGCAGCTC GCTCCAGTCC CCTCACCCAG GGCTTCCTCA CCAGGGCATG 
ATCTTCGTGA AACCCATGTC ACCAGCCCGA GCACTGCAGC AAACCTCAAT ACACGGACAG 
CCCGTCACCC TTATCAGCAT ACCACAGGCT TTAGTTACCA CACCTAAAGG CGGTCAAGCT 
TTCCAGCAGA GTTTCTTTCA CACACCCGTC TCGTTCCCTA CTGTAAACAC CACGGCTCCC 
AAAAAGATCT ACATCCCTCA GAGAAAGCTG GACGTGAGCC CCGAGGAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334620.1
CDS	304..3057
Translation	MSSTLSEGQTLIKKSLSPSKATSTNNKGHVFVEPQTPLKNSNKASTSEAALPETLKIMGPLTTPTKVLDAPSSDPWTPTSNLKMLISAASPEIRNREKERAVDSSESENSQETEQGEEVEKLHISRKDKSLGLLCYKFLARYPNYPNPALNNGISLDDVAAELHVERRRIYDIMNVLESLNMVSRLAKNRYTWHGRVKLAQTLAVLKRAGKENRYEQLMQQIRQRSQEREEREFDLDGEEKENEEMSSFEVDGDSGLADLPGADSKAASANSRKDKSLRVMSQKFVMLFLVSSPPVVSLDVAAKILIGEDHVVDQDKNKFKTKIRRLYDIANVLSSLELIKKVHVTEDKGRKPAFKWTGPEDIPSPKDLEISTTSSAPKPLESRSSVENCAKNLFSSPGTKRGFTRHHSLVKLVKSIQDDRRKINSAPSSPIKMTGDSADSDFYTTKMAHLAAICKKHLDEQSADGRPNNAVTDSSQSSKQPESTSASNHGPPGMQIPVLPAGAISYLPTKCSPIIPLLIPQHQTGGPYAVYMHPTSLRPQPTSLAVRSMTFESPVGANAKTSPATLTSNNQTNQSSSYGKEQTSPVNLKRASGEKSSVGSPSKMQRTEPKSVSPKLCEILQARLKARRGALTSNRPSARALHLEFSKPSESQPTVQTGTASLEHSLETFLEKEEKSQTSDNEAGLTPVRQPHSQPQKLSAPFQDMVLPSGPIHTETLIPAGYLIPISQQSIVNFREPQCSNESSKASTPTYNIYHTPTAGSRPAFPQEVTPTRLPLHRIPPISPFPSHGHRLHSPSPAILNFTLQNLGLIPGSVTPNPHTPEQSSSLQSPHPGLPHQGMIFVKPMSPARALQQTSIHGQPVTLISIPQALVTTPKGGQAFQQSFFHTPVSFPTVNTTAPKKIYIPQRKLDVSPEEI

Target ORF information:

RefSeq Version	NM_001347691.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio E2F transcription factor 8 (e2f8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347691.1

ORF Insert Sequence:

ATGTCCAGTA CATTATCAGA AGGTCAAACA TTAATCAAGA AATCTTTAAG TCCATCAAAG 
GCAACATCTA CAAATAACAA GGGGCATGTT TTTGTGGAGC CACAAACGCC ACTAAAAAAC 
TCAAATAAAG CTTCAACATC TGAAGCTGCT CTACCGGAGA CCCTTAAAAT CATGGGGCCC 
CTGACAACGC CTACTAAGGT CCTAGATGCA CCTTCAAGCG ACCCTTGGAC ACCCACTTCT 
AACCTAAAAA TGCTTATCAG TGCAGCCAGT CCTGAGATCC GCAACCGCGA GAAAGAACGT 
GCCGTGGACA GCAGCGAATC CGAGAACTCA CAGGAGACCG AGCAAGGGGA AGAAGTAGAA 
AAGCTCCATA TAAGCCGTAA AGACAAAAGC CTTGGTTTGT TGTGTTACAA GTTCCTTGCA 
AGGTACCCAA ACTACCCCAA CCCTGCTCTA AACAACGGTA TCAGCCTTGA TGATGTGGCA 
GCAGAACTGC ACGTGGAAAG ACGTCGCATC TACGACATCA TGAATGTCCT TGAAAGCCTT 
AACATGGTCA GCCGGCTGGC AAAGAATCGC TACACTTGGC ATGGTCGAGT AAAACTTGCA 
CAGACGCTTG CTGTGCTGAA ACGGGCCGGA AAAGAGAACC GCTATGAACA GCTTATGCAG 
CAGATCCGAC AAAGGAGTCA AGAGCGAGAA GAGAGAGAGT TCGATTTGGA TGGAGAGGAG 
AAGGAGAATG AAGAAATGTC CAGTTTTGAA GTTGACGGAG ACTCTGGGCT GGCGGATCTT 
CCAGGAGCGG ATTCTAAAGC AGCTTCTGCG AACAGTCGGA AAGACAAATC TCTGCGAGTG 
ATGAGTCAGA AGTTTGTCAT GTTGTTCCTG GTGTCCAGTC CTCCTGTGGT TAGTCTGGAT 
GTAGCTGCCA AAATCCTAAT CGGTGAAGAT CATGTGGTGG ACCAGGACAA AAACAAGTTC 
AAAACCAAGA TCCGTAGACT TTATGACATT GCGAATGTTT TGAGCAGCCT AGAGCTGATT 
AAGAAAGTGC ACGTGACTGA AGATAAAGGA AGAAAGCCTG CTTTCAAATG GACTGGGCCA 
GAAGACATAC CATCTCCAAA GGATCTGGAG ATCTCCACTA CATCCTCAGC ACCAAAGCCT 
CTCGAGTCTC GTTCCTCTGT TGAAAACTGT GCTAAAAATC TGTTTTCCTC CCCTGGAACA 
AAGCGAGGTT TTACCCGCCA TCATTCTCTA GTCAAGCTGG TTAAGAGCAT TCAGGATGAC 
CGCAGGAAAA TCAACTCTGC GCCTTCTAGT CCAATTAAAA TGACAGGTGA TTCTGCAGAC 
AGTGATTTCT ATACTACCAA AATGGCCCAT CTGGCAGCTA TCTGCAAAAA GCACCTCGAT 
GAACAGTCAG CAGACGGTCG ACCTAACAAT GCGGTGACAG ACTCTTCACA GTCATCTAAA 
CAGCCTGAAT CCACATCTGC TAGTAACCAT GGGCCTCCAG GGATGCAGAT CCCAGTTCTG 
CCAGCAGGAG CAATTTCATA CCTTCCCACA AAATGTTCCC CGATCATCCC ACTTCTGATA 
CCTCAGCACC AGACTGGTGG GCCGTATGCC GTTTACATGC ACCCAACGTC TCTCAGACCA 
CAGCCCACCA GCCTGGCTGT CCGTTCAATG ACATTCGAGA GTCCAGTTGG CGCAAATGCA 
AAGACATCTC CGGCTACTTT GACTAGCAAT AACCAGACAA ACCAGTCGTC ATCGTATGGT 
AAAGAGCAGA CAAGTCCTGT GAACCTTAAA CGGGCAAGTG GAGAAAAGAG CTCCGTGGGA 
AGTCCATCCA AAATGCAACG GACAGAGCCA AAGAGCGTTT CTCCAAAGCT GTGTGAGATT 
CTACAAGCAC GTCTGAAGGC CCGTAGAGGA GCTTTGACCT CCAACCGCCC TTCCGCAAGA 
GCTCTTCATC TGGAGTTCTC CAAACCCTCT GAAAGCCAGC CAACGGTACA AACCGGCACA 
GCATCTCTGG AGCACAGCCT GGAGACCTTT CTAGAGAAGG AGGAGAAGAG CCAGACCTCA 
GACAATGAGG CAGGACTGAC CCCTGTAAGA CAACCACATT CACAGCCCCA GAAGCTCAGC 
GCTCCCTTTC AGGACATGGT GCTGCCATCT GGACCCATAC ACACAGAGAC ATTGATCCCC 
GCGGGTTATT TGATTCCCAT CTCTCAGCAG TCCATTGTGA ACTTCAGAGA ACCGCAGTGC 
TCCAATGAAA GCTCCAAAGC CTCCACACCA ACATACAACA TCTATCACAC ACCAACAGCA 
GGTTCCAGAC CAGCTTTTCC TCAAGAAGTC ACCCCGACCC GACTCCCTCT TCACCGGATA 
CCGCCCATCT CCCCCTTCCC TTCTCACGGA CATCGCCTCC ACAGCCCAAG TCCTGCTATT 
CTCAACTTCA CCCTGCAGAA TCTGGGCCTC ATTCCTGGCA GTGTTACACC AAACCCACAC 
ACTCCTGAGC AGAGCAGCTC GCTCCAGTCC CCTCACCCAG GGCTTCCTCA CCAGGGCATG 
ATCTTCGTGA AACCCATGTC ACCAGCCCGA GCACTGCAGC AAACCTCAAT ACACGGACAG 
CCCGTCACCC TTATCAGCAT ACCACAGGCT TTAGTTACCA CACCTAAAGG CGGTCAAGCT 
TTCCAGCAGA GTTTCTTTCA CACACCCGTC TCGTTCCCTA CTGTAAACAC CACGGCTCCC 
AAAAAGATCT ACATCCCTCA GAGAAAGCTG GACGTGAGCC CCGAGGAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E2F8 ( XM_689219.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_694311.6 Danio rerio E2F transcription factor 8 (e2f8), mRNA.

Price & Availability↑

CloneID	ODa12735
Clone ID Related Accession (Same CDS sequence)	NM_001347691.1 , XM_689219.8
Accession Version	XM_689219.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transcription factor E2F8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_689219.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGTA CATTATCAGA AGGTCAAACA TTAATCAAGA AATCTTTAAG TCCATCAAAG 
GCAACATCTA CAAATAACAA GGGGCATGTT TTTGTGGAGC CACAAACGCC ACTAAAAAAC 
TCAAATAAAG CTTCAACATC TGAAGCTGCT CTACCGGAGA CCCTTAAAAT CATGGGGCCC 
CTGACAACGC CTACTAAGGT CCTAGATGCA CCTTCAAGCG ACCCTTGGAC ACCCACTTCT 
AACCTAAAAA TGCTTATCAG TGCAGCCAGT CCTGAGATCC GCAACCGCGA GAAAGAACGT 
GCCGTGGACA GCAGCGAATC CGAGAACTCA CAGGAGACCG AGCAAGGGGA AGAAGTAGAA 
AAGCTCCATA TAAGCCGTAA AGACAAAAGC CTTGGTTTGT TGTGTTACAA GTTCCTTGCA 
AGGTACCCAA ACTACCCCAA CCCTGCTCTA AACAACGGTA TCAGCCTTGA TGATGTGGCA 
GCAGAACTGC ACGTGGAAAG ACGTCGCATC TACGACATCA TGAATGTCCT TGAAAGCCTT 
AACATGGTCA GCCGGCTGGC AAAGAATCGC TACACTTGGC ATGGTCGAGT AAAACTTGCA 
CAGACGCTTG CTGTGCTGAA ACGGGCCGGA AAAGAGAACC GCTATGAACA GCTTATGCAG 
CAGATCCGAC AAAGGAGTCA AGAGCGAGAA GAGAGAGAGT TCGATTTGGA TGGAGAGGAG 
AAGGAGAATG AAGAAATGTC CAGTTTTGAA GTTGACGGAG ACTCTGGGCT GGCGGATCTT 
CCAGGAGCGG ATTCTAAAGC AGCTTCTGCG AACAGTCGGA AAGACAAATC TCTGCGAGTG 
ATGAGTCAGA AGTTTGTCAT GTTGTTCCTG GTGTCCAGTC CTCCTGTGGT TAGTCTGGAT 
GTAGCTGCCA AAATCCTAAT CGGTGAAGAT CATGTGGTGG ACCAGGACAA AAACAAGTTC 
AAAACCAAGA TCCGTAGACT TTATGACATT GCGAATGTTT TGAGCAGCCT AGAGCTGATT 
AAGAAAGTGC ACGTGACTGA AGATAAAGGA AGAAAGCCTG CTTTCAAATG GACTGGGCCA 
GAAGACATAC CATCTCCAAA GGATCTGGAG ATCTCCACTA CATCCTCAGC ACCAAAGCCT 
CTCGAGTCTC GTTCCTCTGT TGAAAACTGT GCTAAAAATC TGTTTTCCTC CCCTGGAACA 
AAGCGAGGTT TTACCCGCCA TCATTCTCTA GTCAAGCTGG TTAAGAGCAT TCAGGATGAC 
CGCAGGAAAA TCAACTCTGC GCCTTCTAGT CCAATTAAAA TGACAGGTGA TTCTGCAGAC 
AGTGATTTCT ATACTACCAA AATGGCCCAT CTGGCAGCTA TCTGCAAAAA GCACCTCGAT 
GAACAGTCAG CAGACGGTCG ACCTAACAAT GCGGTGACAG ACTCTTCACA GTCATCTAAA 
CAGCCTGAAT CCACATCTGC TAGTAACCAT GGGCCTCCAG GGATGCAGAT CCCAGTTCTG 
CCAGCAGGAG CAATTTCATA CCTTCCCACA AAATGTTCCC CGATCATCCC ACTTCTGATA 
CCTCAGCACC AGACTGGTGG GCCGTATGCC GTTTACATGC ACCCAACGTC TCTCAGACCA 
CAGCCCACCA GCCTGGCTGT CCGTTCAATG ACATTCGAGA GTCCAGTTGG CGCAAATGCA 
AAGACATCTC CGGCTACTTT GACTAGCAAT AACCAGACAA ACCAGTCGTC ATCGTATGGT 
AAAGAGCAGA CAAGTCCTGT GAACCTTAAA CGGGCAAGTG GAGAAAAGAG CTCCGTGGGA 
AGTCCATCCA AAATGCAACG GACAGAGCCA AAGAGCGTTT CTCCAAAGCT GTGTGAGATT 
CTACAAGCAC GTCTGAAGGC CCGTAGAGGA GCTTTGACCT CCAACCGCCC TTCCGCAAGA 
GCTCTTCATC TGGAGTTCTC CAAACCCTCT GAAAGCCAGC CAACGGTACA AACCGGCACA 
GCATCTCTGG AGCACAGCCT GGAGACCTTT CTAGAGAAGG AGGAGAAGAG CCAGACCTCA 
GACAATGAGG CAGGACTGAC CCCTGTAAGA CAACCACATT CACAGCCCCA GAAGCTCAGC 
GCTCCCTTTC AGGACATGGT GCTGCCATCT GGACCCATAC ACACAGAGAC ATTGATCCCC 
GCGGGTTATT TGATTCCCAT CTCTCAGCAG TCCATTGTGA ACTTCAGAGA ACCGCAGTGC 
TCCAATGAAA GCTCCAAAGC CTCCACACCA ACATACAACA TCTATCACAC ACCAACAGCA 
GGTTCCAGAC CAGCTTTTCC TCAAGAAGTC ACCCCGACCC GACTCCCTCT TCACCGGATA 
CCGCCCATCT CCCCCTTCCC TTCTCACGGA CATCGCCTCC ACAGCCCAAG TCCTGCTATT 
CTCAACTTCA CCCTGCAGAA TCTGGGCCTC ATTCCTGGCA GTGTTACACC AAACCCACAC 
ACTCCTGAGC AGAGCAGCTC GCTCCAGTCC CCTCACCCAG GGCTTCCTCA CCAGGGCATG 
ATCTTCGTGA AACCCATGTC ACCAGCCCGA GCACTGCAGC AAACCTCAAT ACACGGACAG 
CCCGTCACCC TTATCAGCAT ACCACAGGCT TTAGTTACCA CACCTAAAGG CGGTCAAGCT 
TTCCAGCAGA GTTTCTTTCA CACACCCGTC TCGTTCCCTA CTGTAAACAC CACGGCTCCC 
AAAAAGATCT ACATCCCTCA GAGAAAGCTG GACGTGAGCC CCGAGGAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_694311.6
CDS	291..3044
Translation	MSSTLSEGQTLIKKSLSPSKATSTNNKGHVFVEPQTPLKNSNKASTSEAALPETLKIMGPLTTPTKVLDAPSSDPWTPTSNLKMLISAASPEIRNREKERAVDSSESENSQETEQGEEVEKLHISRKDKSLGLLCYKFLARYPNYPNPALNNGISLDDVAAELHVERRRIYDIMNVLESLNMVSRLAKNRYTWHGRVKLAQTLAVLKRAGKENRYEQLMQQIRQRSQEREEREFDLDGEEKENEEMSSFEVDGDSGLADLPGADSKAASANSRKDKSLRVMSQKFVMLFLVSSPPVVSLDVAAKILIGEDHVVDQDKNKFKTKIRRLYDIANVLSSLELIKKVHVTEDKGRKPAFKWTGPEDIPSPKDLEISTTSSAPKPLESRSSVENCAKNLFSSPGTKRGFTRHHSLVKLVKSIQDDRRKINSAPSSPIKMTGDSADSDFYTTKMAHLAAICKKHLDEQSADGRPNNAVTDSSQSSKQPESTSASNHGPPGMQIPVLPAGAISYLPTKCSPIIPLLIPQHQTGGPYAVYMHPTSLRPQPTSLAVRSMTFESPVGANAKTSPATLTSNNQTNQSSSYGKEQTSPVNLKRASGEKSSVGSPSKMQRTEPKSVSPKLCEILQARLKARRGALTSNRPSARALHLEFSKPSESQPTVQTGTASLEHSLETFLEKEEKSQTSDNEAGLTPVRQPHSQPQKLSAPFQDMVLPSGPIHTETLIPAGYLIPISQQSIVNFREPQCSNESSKASTPTYNIYHTPTAGSRPAFPQEVTPTRLPLHRIPPISPFPSHGHRLHSPSPAILNFTLQNLGLIPGSVTPNPHTPEQSSSLQSPHPGLPHQGMIFVKPMSPARALQQTSIHGQPVTLISIPQALVTTPKGGQAFQQSFFHTPVSFPTVNTTAPKKIYIPQRKLDVSPEEI

Target ORF information:

RefSeq Version	XM_689219.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio E2F transcription factor 8 (e2f8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_689219.8

ORF Insert Sequence:

ATGTCCAGTA CATTATCAGA AGGTCAAACA TTAATCAAGA AATCTTTAAG TCCATCAAAG 
GCAACATCTA CAAATAACAA GGGGCATGTT TTTGTGGAGC CACAAACGCC ACTAAAAAAC 
TCAAATAAAG CTTCAACATC TGAAGCTGCT CTACCGGAGA CCCTTAAAAT CATGGGGCCC 
CTGACAACGC CTACTAAGGT CCTAGATGCA CCTTCAAGCG ACCCTTGGAC ACCCACTTCT 
AACCTAAAAA TGCTTATCAG TGCAGCCAGT CCTGAGATCC GCAACCGCGA GAAAGAACGT 
GCCGTGGACA GCAGCGAATC CGAGAACTCA CAGGAGACCG AGCAAGGGGA AGAAGTAGAA 
AAGCTCCATA TAAGCCGTAA AGACAAAAGC CTTGGTTTGT TGTGTTACAA GTTCCTTGCA 
AGGTACCCAA ACTACCCCAA CCCTGCTCTA AACAACGGTA TCAGCCTTGA TGATGTGGCA 
GCAGAACTGC ACGTGGAAAG ACGTCGCATC TACGACATCA TGAATGTCCT TGAAAGCCTT 
AACATGGTCA GCCGGCTGGC AAAGAATCGC TACACTTGGC ATGGTCGAGT AAAACTTGCA 
CAGACGCTTG CTGTGCTGAA ACGGGCCGGA AAAGAGAACC GCTATGAACA GCTTATGCAG 
CAGATCCGAC AAAGGAGTCA AGAGCGAGAA GAGAGAGAGT TCGATTTGGA TGGAGAGGAG 
AAGGAGAATG AAGAAATGTC CAGTTTTGAA GTTGACGGAG ACTCTGGGCT GGCGGATCTT 
CCAGGAGCGG ATTCTAAAGC AGCTTCTGCG AACAGTCGGA AAGACAAATC TCTGCGAGTG 
ATGAGTCAGA AGTTTGTCAT GTTGTTCCTG GTGTCCAGTC CTCCTGTGGT TAGTCTGGAT 
GTAGCTGCCA AAATCCTAAT CGGTGAAGAT CATGTGGTGG ACCAGGACAA AAACAAGTTC 
AAAACCAAGA TCCGTAGACT TTATGACATT GCGAATGTTT TGAGCAGCCT AGAGCTGATT 
AAGAAAGTGC ACGTGACTGA AGATAAAGGA AGAAAGCCTG CTTTCAAATG GACTGGGCCA 
GAAGACATAC CATCTCCAAA GGATCTGGAG ATCTCCACTA CATCCTCAGC ACCAAAGCCT 
CTCGAGTCTC GTTCCTCTGT TGAAAACTGT GCTAAAAATC TGTTTTCCTC CCCTGGAACA 
AAGCGAGGTT TTACCCGCCA TCATTCTCTA GTCAAGCTGG TTAAGAGCAT TCAGGATGAC 
CGCAGGAAAA TCAACTCTGC GCCTTCTAGT CCAATTAAAA TGACAGGTGA TTCTGCAGAC 
AGTGATTTCT ATACTACCAA AATGGCCCAT CTGGCAGCTA TCTGCAAAAA GCACCTCGAT 
GAACAGTCAG CAGACGGTCG ACCTAACAAT GCGGTGACAG ACTCTTCACA GTCATCTAAA 
CAGCCTGAAT CCACATCTGC TAGTAACCAT GGGCCTCCAG GGATGCAGAT CCCAGTTCTG 
CCAGCAGGAG CAATTTCATA CCTTCCCACA AAATGTTCCC CGATCATCCC ACTTCTGATA 
CCTCAGCACC AGACTGGTGG GCCGTATGCC GTTTACATGC ACCCAACGTC TCTCAGACCA 
CAGCCCACCA GCCTGGCTGT CCGTTCAATG ACATTCGAGA GTCCAGTTGG CGCAAATGCA 
AAGACATCTC CGGCTACTTT GACTAGCAAT AACCAGACAA ACCAGTCGTC ATCGTATGGT 
AAAGAGCAGA CAAGTCCTGT GAACCTTAAA CGGGCAAGTG GAGAAAAGAG CTCCGTGGGA 
AGTCCATCCA AAATGCAACG GACAGAGCCA AAGAGCGTTT CTCCAAAGCT GTGTGAGATT 
CTACAAGCAC GTCTGAAGGC CCGTAGAGGA GCTTTGACCT CCAACCGCCC TTCCGCAAGA 
GCTCTTCATC TGGAGTTCTC CAAACCCTCT GAAAGCCAGC CAACGGTACA AACCGGCACA 
GCATCTCTGG AGCACAGCCT GGAGACCTTT CTAGAGAAGG AGGAGAAGAG CCAGACCTCA 
GACAATGAGG CAGGACTGAC CCCTGTAAGA CAACCACATT CACAGCCCCA GAAGCTCAGC 
GCTCCCTTTC AGGACATGGT GCTGCCATCT GGACCCATAC ACACAGAGAC ATTGATCCCC 
GCGGGTTATT TGATTCCCAT CTCTCAGCAG TCCATTGTGA ACTTCAGAGA ACCGCAGTGC 
TCCAATGAAA GCTCCAAAGC CTCCACACCA ACATACAACA TCTATCACAC ACCAACAGCA 
GGTTCCAGAC CAGCTTTTCC TCAAGAAGTC ACCCCGACCC GACTCCCTCT TCACCGGATA 
CCGCCCATCT CCCCCTTCCC TTCTCACGGA CATCGCCTCC ACAGCCCAAG TCCTGCTATT 
CTCAACTTCA CCCTGCAGAA TCTGGGCCTC ATTCCTGGCA GTGTTACACC AAACCCACAC 
ACTCCTGAGC AGAGCAGCTC GCTCCAGTCC CCTCACCCAG GGCTTCCTCA CCAGGGCATG 
ATCTTCGTGA AACCCATGTC ACCAGCCCGA GCACTGCAGC AAACCTCAAT ACACGGACAG 
CCCGTCACCC TTATCAGCAT ACCACAGGCT TTAGTTACCA CACCTAAAGG CGGTCAAGCT 
TTCCAGCAGA GTTTCTTTCA CACACCCGTC TCGTTCCCTA CTGTAAACAC CACGGCTCCC 
AAAAAGATCT ACATCCCTCA GAGAAAGCTG GACGTGAGCC CCGAGGAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide analysis reveals NRP1 as a direct HIF1?-E2F7 target in the regulation of motorneuron guidance in vivo.
Nucleic acids research44(8)3549-66(2016 05)
de Bruin A,A Cornelissen PW,Kirchmaier BC,Mokry M,Iich E,Nirmala E,Liang KH,D V?gh AM,Scholman KT,Groot Koerkamp MJ,Holstege FC,Cuppen E,Schulte-Merker S,Bakker WJ

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

E2F8 promotes hepatic steatosis through FABP3 expression in diet-induced obesity in zebrafish.
Nutrition & metabolism1217(2015)
Shimada Y,Kuninaga S,Ariyoshi M,Zhang B,Shiina Y,Takahashi Y,Umemoto N,Nishimura Y,Enari H,Tanaka T

Evolution of the vertebrate Pax4/6 class of genes with focus on its novel member, the Pax10 gene.
Genome biology and evolution6(7)1635-51(2014 Jun)
Feiner N,Meyer A,Kuraku S

Atypical E2fs control lymphangiogenesis through transcriptional regulation of Ccbe1 and Flt4.
PloS one8(9)e73693(2013)
Weijts BG,van Impel A,Schulte-Merker S,de Bruin A