mep1a.2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mep1a.2
Entrez Gene ID	565535
Full Name	meprin A, alpha (PABA peptide hydrolase), tandem duplicate 2
Synonyms	si:dkey-30j22.6
General protein information	Preferred Names meprin A, alpha (PABA peptide hydrolase), tandem duplicate 2 Names meprin A subunit alpha MEP1Ac mephrin A, alpha.2 meprin A, alpha, tandem duplicate 2 meprin A, alpha.2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001128727.1	NP_001122199.1	meprin A subunit alpha precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MEP1A	XP_003587735.2
Mus musculus (house mouse)	Mep1a	NP_032611.2
Xenopus tropicalis (tropical clawed frog)	mep1a	NP_001015767.1
Gallus gallus (chicken)	MEP1A	NP_001264650.1
	MEP1A	XP_001103444.1
Danio rerio (zebrafish)	mep1a.2	NP_001122199.1
Canis lupus familiaris (dog)	MEP1A	XP_538947.3
Rattus norvegicus (Norway rat)	Mep1a	NP_037275.1
Homo sapiens (human)	MEP1A	NP_005579.2
Pan troglodytes (chimpanzee)	MEP1A	XP_518520.3

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Let it flow: Morpholino knockdown in zebrafish embryos reveals a pro-angiogenic effect of the metalloprotease meprin alpha2.
Sch?tte A, Hedrich J, St?cker W, Becker-Pauly C
PloS one5(1)e8835(2010 Jan)

Two alpha subunits and one beta subunit of meprin zinc-endopeptidases are differentially expressed in the zebrafish Danio rerio.
Sch?tte A, Lottaz D, Sterchi EE, St?cker W, Becker-Pauly C
Biological chemistry388(5)523-31(2007 May)

The evolution of the vertebrate metzincins; insights from Ciona intestinalis and Danio rerio.
Huxley-Jones J, Clarke TK, Beck C, Toubaris G, Robertson DL, Boot-Handford RP
BMC evolutionary biology763(2007 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mep1a.2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mep1a.2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02159	NM_001128727.1 Latest version!	Danio rerio meprin A, alpha (PABA peptide hydrolase), tandem duplicate 2 (mep1a.2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mep1a.2 ( NM_001128727.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001122199.1 Danio rerio meprin A, alpha (PABA peptide hydrolase), tandem duplicate 2 (mep1a.2), mRNA.

Price & Availability↑

CloneID	ODa02159
Clone ID Related Accession (Same CDS sequence)	NM_001128727.1
Accession Version	NM_001128727.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	meprin A subunit alpha precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC163930.1. On Sep 9, 2008 this sequence version replaced XM_688815.2. ##Evidence-Data-START## Transcript exon combination :: BC163930.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END##

ATGGACTCCA TGTGGAGAAT CAGCCTCTTC TTTGTACTTC TAGCGCTCAA GGCATGTGCC 
CTTCCAGCAC AATATGGCGA AGATGCAGAT GCAGGTGAAT TACGGGAGGA CATTCTTGAA 
ATTAATTTAG ATTCCCAAAG AGAATTGTTT GAAGGAGATA TTGCAGGTGA TCCACGAAGA 
AATGCAATAA TAGATGAAAA AGCAAGATGG CAGTTTCCCA TTCCATATAT ACTCACAGAC 
ACATTGGATC TGAATGCAAA AGGAGTGATC CTTCAAGCAC TTGAAATGTA TCGTCTGAAG 
TCTTGTGTGG ACTTTAAGCC CTATGAAGGA GAAAGCACCT ACATTTCTTT CACAAAACTG 
GATGGATGTT GGTCATTTGT AGGAGATTTA AAGACAGGCC AGAACGTCTC CATCGGGGAG 
AGATGTGACA CTAAAGCCAT TGTAGAACAT GAGCTTCTCC ATGCACTGGG TTTCTATCAT 
GAGCAATCCC GCTCAGACCG GGACGACTAT GTCAAAATCT GGTGGGATCA AATCATTGAA 
GGAAAGGAGC ACAATTTTAA TAAGTACGAG GATGATTTTA TCACTGATTT GAACACACCC 
TATGATTATG AGTCCATCAT GCACTACAGG CCTCTGTCGT TCAACAAGGA TCCTGATATT 
CCTACTATAA CCACCACCAT CCCTGCCTTC AACAACATCA TAGGACAGCG CTTAGACTTC 
AGTGCACTTG ATCTGGAGAG ACTCAATCGC ATGTATGAAT GCACTGCAAC CCACACTCTG 
CTGGATCAGT GTGCCTTTGA GCAGATCAAC ATTTGTGGAA TGATTCAGAA TGATGAGGAT 
GATGCTGACT GGGTCCAGAC TTTGAGCTCT ACAGATTTAA AGGATCACAC ACTTGGAGGA 
CAATGCAGAG ATTCAGGTTA TTTCATGAAG TTTGACACCG ATAACAAGAC CGAGGGACAC 
AGTGCTTTGC TGGAGTCACG GATTCTTTAC CCCAAGAGGA ACCAGCAGTG CCTTGAGTTC 
TTCTACAGGA TGAGTGGAGA GCCAGGCGAC AAGCTCATCA TCTGGGTCAG AACTGACGAT 
GGAACTGGCA ATGTGAACAA AGTCAGGAAA GTTCACACAA TCACAGGTCA TGGGGATAAC 
TCTTGGAACA TTGCACAAGT CACTCTAAAT GTTAAGGAAA AATTCCGGTA CTTCTTCCAG 
GGTATTGTGG GCCCCAACAA AACATCAGGG ATCTTTATAG ATGACATTAT TCTGACTGAA 
ACATCATGTC CAAACACTGT CTGGAGGATC CAGAACTTTA CCAACCTCCT CAATACTCTC 
CCACATGATG CCAAAGTTCA GAGTGAACGT TTTTACAACT CTGAGGGTTA CGCTTATGGA 
ATTAATGTTT ATCCAAATGG CAGGGTGAAT TCATCTAAAG AGTTTGTAGG GATTACGTTT 
AATCTTTTCG GTGGTGAGAA TGATGCAGTA TTAGAATGGC CTGCGGTGAA TCGGCAAGTC 
ACTGTTACAG CAAAGGACCA AAATCCAGAT GCAACCCTGC AAATGTCCAA CAGCAGAAGC 
TTTACCACTG ATGCTGACAT GCGGTGGAAT AAACCATCTA CATTTGAACA ATGGGATGAT 
AGTTGCCTTT GCTTCAGAGG ACCAGAGTTT GGCTGGGGTA CCTTCATATC TCACGATCAG 
CTCCGCAGGA GAGACTTTCT CAAGAACGAC GACCTGATCA TCACTATTAA TTTTGATGAT 
TTGGCACATC TTGTAAAATC TGAAGTTCCA ACCAAAGTCT CAAACAACCC CCAGCCTGCA 
GAAAAAAACA TTGAGAAACC AAAAATCCGA CAACCACGAG CAATCAGTGA TCTGTGTCAA 
CCAAACCCCT GCCAAAATGG AGGAGCGTGT GTCACACATC AGGGGAAAGC AACATGCAGG 
TGTGCCTCTG GACAGGCTGT TGTGTACACA GGAGACACTT GTGAGAAACA ACACATTGAC 
GGAGGAATTA TGGGAGTTTT GATTGGGGGT GCTGTTGGAA CTGTAGCACT TACTGTTGCT 
ATCATTGCAG TGATCTACAG GCAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122199.1
CDS	33..2102
Translation	MDSMWRISLFFVLLALKACALPAQYGEDADAGELREDILEINLDSQRELFEGDIAGDPRRNAIIDEKARWQFPIPYILTDTLDLNAKGVILQALEMYRLKSCVDFKPYEGESTYISFTKLDGCWSFVGDLKTGQNVSIGERCDTKAIVEHELLHALGFYHEQSRSDRDDYVKIWWDQIIEGKEHNFNKYEDDFITDLNTPYDYESIMHYRPLSFNKDPDIPTITTTIPAFNNIIGQRLDFSALDLERLNRMYECTATHTLLDQCAFEQINICGMIQNDEDDADWVQTLSSTDLKDHTLGGQCRDSGYFMKFDTDNKTEGHSALLESRILYPKRNQQCLEFFYRMSGEPGDKLIIWVRTDDGTGNVNKVRKVHTITGHGDNSWNIAQVTLNVKEKFRYFFQGIVGPNKTSGIFIDDIILTETSCPNTVWRIQNFTNLLNTLPHDAKVQSERFYNSEGYAYGINVYPNGRVNSSKEFVGITFNLFGGENDAVLEWPAVNRQVTVTAKDQNPDATLQMSNSRSFTTDADMRWNKPSTFEQWDDSCLCFRGPEFGWGTFISHDQLRRRDFLKNDDLIITINFDDLAHLVKSEVPTKVSNNPQPAEKNIEKPKIRQPRAISDLCQPNPCQNGGACVTHQGKATCRCASGQAVVYTGDTCEKQHIDGGIMGVLIGGAVGTVALTVAIIAVIYRQK

Target ORF information:

RefSeq Version	NM_001128727.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio meprin A, alpha (PABA peptide hydrolase), tandem duplicate 2 (mep1a.2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128727.1

ORF Insert Sequence:

ATGGACTCCA TGTGGAGAAT CAGCCTCTTC TTTGTACTTC TAGCGCTCAA GGCATGTGCC 
CTTCCAGCAC AATATGGCGA AGATGCAGAT GCAGGTGAAT TACGGGAGGA CATTCTTGAA 
ATTAATTTAG ATTCCCAAAG AGAATTGTTT GAAGGAGATA TTGCAGGTGA TCCACGAAGA 
AATGCAATAA TAGATGAAAA AGCAAGATGG CAGTTTCCCA TTCCATATAT ACTCACAGAC 
ACATTGGATC TGAATGCAAA AGGAGTGATC CTTCAAGCAC TTGAAATGTA TCGTCTGAAG 
TCTTGTGTGG ACTTTAAGCC CTATGAAGGA GAAAGCACCT ACATTTCTTT CACAAAACTG 
GATGGATGTT GGTCATTTGT AGGAGATTTA AAGACAGGCC AGAACGTCTC CATCGGGGAG 
AGATGTGACA CTAAAGCCAT TGTAGAACAT GAGCTTCTCC ATGCACTGGG TTTCTATCAT 
GAGCAATCCC GCTCAGACCG GGACGACTAT GTCAAAATCT GGTGGGATCA AATCATTGAA 
GGAAAGGAGC ACAATTTTAA TAAGTACGAG GATGATTTTA TCACTGATTT GAACACACCC 
TATGATTATG AGTCCATCAT GCACTACAGG CCTCTGTCGT TCAACAAGGA TCCTGATATT 
CCTACTATAA CCACCACCAT CCCTGCCTTC AACAACATCA TAGGACAGCG CTTAGACTTC 
AGTGCACTTG ATCTGGAGAG ACTCAATCGC ATGTATGAAT GCACTGCAAC CCACACTCTG 
CTGGATCAGT GTGCCTTTGA GCAGATCAAC ATTTGTGGAA TGATTCAGAA TGATGAGGAT 
GATGCTGACT GGGTCCAGAC TTTGAGCTCT ACAGATTTAA AGGATCACAC ACTTGGAGGA 
CAATGCAGAG ATTCAGGTTA TTTCATGAAG TTTGACACCG ATAACAAGAC CGAGGGACAC 
AGTGCTTTGC TGGAGTCACG GATTCTTTAC CCCAAGAGGA ACCAGCAGTG CCTTGAGTTC 
TTCTACAGGA TGAGTGGAGA GCCAGGCGAC AAGCTCATCA TCTGGGTCAG AACTGACGAT 
GGAACTGGCA ATGTGAACAA AGTCAGGAAA GTTCACACAA TCACAGGTCA TGGGGATAAC 
TCTTGGAACA TTGCACAAGT CACTCTAAAT GTTAAGGAAA AATTCCGGTA CTTCTTCCAG 
GGTATTGTGG GCCCCAACAA AACATCAGGG ATCTTTATAG ATGACATTAT TCTGACTGAA 
ACATCATGTC CAAACACTGT CTGGAGGATC CAGAACTTTA CCAACCTCCT CAATACTCTC 
CCACATGATG CCAAAGTTCA GAGTGAACGT TTTTACAACT CTGAGGGTTA CGCTTATGGA 
ATTAATGTTT ATCCAAATGG CAGGGTGAAT TCATCTAAAG AGTTTGTAGG GATTACGTTT 
AATCTTTTCG GTGGTGAGAA TGATGCAGTA TTAGAATGGC CTGCGGTGAA TCGGCAAGTC 
ACTGTTACAG CAAAGGACCA AAATCCAGAT GCAACCCTGC AAATGTCCAA CAGCAGAAGC 
TTTACCACTG ATGCTGACAT GCGGTGGAAT AAACCATCTA CATTTGAACA ATGGGATGAT 
AGTTGCCTTT GCTTCAGAGG ACCAGAGTTT GGCTGGGGTA CCTTCATATC TCACGATCAG 
CTCCGCAGGA GAGACTTTCT CAAGAACGAC GACCTGATCA TCACTATTAA TTTTGATGAT 
TTGGCACATC TTGTAAAATC TGAAGTTCCA ACCAAAGTCT CAAACAACCC CCAGCCTGCA 
GAAAAAAACA TTGAGAAACC AAAAATCCGA CAACCACGAG CAATCAGTGA TCTGTGTCAA 
CCAAACCCCT GCCAAAATGG AGGAGCGTGT GTCACACATC AGGGGAAAGC AACATGCAGG 
TGTGCCTCTG GACAGGCTGT TGTGTACACA GGAGACACTT GTGAGAAACA ACACATTGAC 
GGAGGAATTA TGGGAGTTTT GATTGGGGGT GCTGTTGGAA CTGTAGCACT TACTGTTGCT 
ATCATTGCAG TGATCTACAG GCAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Let it flow: Morpholino knockdown in zebrafish embryos reveals a pro-angiogenic effect of the metalloprotease meprin alpha2.
PloS one5(1)e8835(2010 Jan)
Sch?tte A,Hedrich J,St?cker W,Becker-Pauly C

Two alpha subunits and one beta subunit of meprin zinc-endopeptidases are differentially expressed in the zebrafish Danio rerio.
Biological chemistry388(5)523-31(2007 May)
Sch?tte A,Lottaz D,Sterchi EE,St?cker W,Becker-Pauly C

The evolution of the vertebrate metzincins; insights from Ciona intestinalis and Danio rerio.
BMC evolutionary biology763(2007 Apr)
Huxley-Jones J,Clarke TK,Beck C,Toubaris G,Robertson DL,Boot-Handford RP