p4ha1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	p4ha1a
Entrez Gene ID	562774
Full Name	prolyl 4-hydroxylase, alpha polypeptide I a
Synonyms	SI:zC146F4.4,si:ch211-146f4.5,zC146F4.5
General protein information	Preferred Names prolyl 4-hydroxylase, alpha polypeptide I a Names prolyl 4-hydroxylase, alpha polypeptide I a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009306501.3	XP_009304776.1	prolyl 4-hydroxylase, alpha polypeptide I a isoform X1
XM_009306502.2	XP_009304777.1	prolyl 4-hydroxylase, alpha polypeptide I a isoform X2
XM_009306502.3	XP_009304777.1	prolyl 4-hydroxylase, alpha polypeptide I a isoform X2
XM_009306501.2	XP_009304776.1	prolyl 4-hydroxylase, alpha polypeptide I a isoform X1
NM_001044998.1	NP_001038463.1	prolyl 4-hydroxylase, alpha polypeptide I a precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	P4HA1	XP_005619064.1
Bos taurus (cattle)	P4HA1	NP_001069238.1
Danio rerio (zebrafish)	p4ha1a	NP_001038463.1
Drosophila melanogaster (fruit fly)	PH4alphaEFB	NP_733371.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000971	XP_309164.4
Rattus norvegicus (Norway rat)	P4ha1	NP_742059.2
Caenorhabditis elegans (roundworm)	dpy-18	NP_499464.1
Xenopus tropicalis (tropical clawed frog)	LOC100490484	XP_002936004.1
	P4HA1	XP_001103971.2
Xenopus tropicalis (tropical clawed frog)	p4ha1	XP_004915844.1
Homo sapiens (human)	P4HA1	NP_001136067.1
Mus musculus (house mouse)	P4ha1	NP_035160.1
Danio rerio (zebrafish)	LOC556879	XP_005156147.1
Caenorhabditis elegans (roundworm)	phy-2	NP_502317.1
Pan troglodytes (chimpanzee)	P4HA1	XP_001140652.1
Gallus gallus (chicken)	P4HA1	NP_001185667.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Deficiency of a transmembrane prolyl 4-hydroxylase in the zebrafish leads to basement membrane defects and compromised kidney function.
Hyv?rinen J, Parikka M, Sormunen R, R?met M, Tryggvason K, Kivirikko KI, Myllyharju J, Koivunen P
The Journal of biological chemistry285(53)42023-32(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following p4ha1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the p4ha1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa35400	NM_001044998.1 Latest version!	Danio rerio prolyl 4-hydroxylase, alpha polypeptide I a (p4ha1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa17646	XM_009306501.3 Latest version!	Danio rerio prolyl 4-hydroxylase, alpha polypeptide I a (p4ha1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa17647	XM_009306502.3 Latest version!	Danio rerio prolyl 4-hydroxylase, alpha polypeptide I a (p4ha1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa17647	XM_009306502.2	Danio rerio prolyl 4-hydroxylase, alpha polypeptide I a (p4ha1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa17646	XM_009306501.2	Danio rerio prolyl 4-hydroxylase, alpha polypeptide I a (p4ha1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

p4ha1a ( NM_001044998.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038463.1 Danio rerio prolyl 4-hydroxylase, alpha polypeptide I a (p4ha1a), mRNA.

Price & Availability↑

CloneID	ODa35400
Clone ID Related Accession (Same CDS sequence)	NM_001044998.1
Accession Version	NM_001044998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	prolyl 4-hydroxylase, alpha polypeptide I a precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL732629.6. On or before Sep 1, 2006 this sequence version replaced XM_681379.1, XM_703353.1, XM_703354.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGATGGGAG TTAGGAGTGT GTGCTGTTTT TTACTCATCT CTCTGCTAAA GTCTTCCTCT 
GCTCACAGCG ACTTCTTCAC TTCCATTGGT CATATGACAG ATCTGTTGTT CACAGAAAAA 
GACCTGGTGA CATCATTGAA AGACTACATT AAAGCAGAGG AGAGCAAACT AGAGCAAGTT 
AAACAGTGGG CAGAGAAGCT TGATGCTTTA ACAGCCACAG CCGTCCAGGA CCCAGAGGGC 
TTTTTGGGTC ATCCTGTCAA TGCCTTTAAA CTCATGAAGA GACTAAACAC AGAATGGGGT 
GAAGTGGAAG ATCTAGTGCT CAAGGACATG TCTGATGGCT TTATCTCAAA CCTGACCATT 
CAGCGCCAGT ACTTCCCCAA TGATGAAGAC CAGAACGGTG CAGCGAAAGC CCTCCTCCGA 
CTGCAGGACA CATATAAACT GGACACACAG ACCATCTCTT CTGGAGACCT GCCTGGTGTT 
AATACAGGTC TGCCATATAA AAGCACCCTG ACGGTAGAGG ACTGTTTTGA ACTAGGAAAA 
ATTGCCTACT CTGACGCAGA CTACTATCAC ACGGAGCTGT GGATGGCCCA GGCGCTCAAG 
CAGCTGGATG AAGGAGAAGA ATCCAGCGTT GAGATTGCCA CCGCTCTGGA TTACCTCAGT 
TACTCTGTCT ACCAACAGGG GGAGCTGGAG AGAGCTCTGG AATATACTAA GAGACTGCTT 
ACTGTGGATG GGAAGAAGGA ATATCTCCCG GAGAAGAGGA AATATGAGAA GCTGTGTAGA 
GGAGAAGGAC TCAAAATGAC TCCTCGGAGA CAGAAGCATT TGTTCTGCCG TTACTTCAAT 
GGTAACAGAC ATCCATTTTA CACAATTGGG CCAGTGAAAC AAGAAGACGA ATGGGACCGT 
CCCCGAATCA TCCGTTATCA TGAGATAATA ACAGAACAAG AAATAGAGAA AATCAAGGAG 
CTCTCCAAAC CAAGGCTGCG CCGAGCCACC ATCTCCAACC CCATTACTGG TGTCCTAGAG 
ACTGCACACT ATCGCATCAG TAAAAGGCGC GCCACCGTTC ATGACCCTCA AACTGGCAAA 
CTCACCACCG CCCAGTACAG AGTCTCCAAG AGTGCGTGGT TGGCGGCCTA TGAGCATCCG 
GTTGTGGATC GCATCAATCA GCGCATTGAA GACATCACAG GCCTCAATGT CAAAACCGCA 
GAAGAGTTAC AGGTGGCGAA TTATGGTGTT GGAGGACAAT ATGAGCCCCA TTTTGACTTT 
GGACGGAAAG ATGAACCAGA TGCCTTTAAA GAGCTGGGCA CAGGGAATCG CATAGCAACC 
TGGCTCTTCT ACATGAGTGA TGTAGCAGCA GGGGGAGCCA CTGTCTTTCC AGAAGTAGGA 
GCTGCAGTAA AGCCACTGAA GGGCACCGCA GTGTTCTGGT ATAATCTGTT TCCCAGTGGA 
GAAGGAGATT ACAGCACGAG ACACGCAGCC TGTCCAGTAC TGGTGGGAAA CAAGTGGGTG 
TCAAATAAAT GGATCCACGA GCGAGGTCAG GAATTCAGGA GACCCTGCGG CCTGAAAGAG 
ACCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038463.1
CDS	1..1569
Translation	MMGVRSVCCFLLISLLKSSSAHSDFFTSIGHMTDLLFTEKDLVTSLKDYIKAEESKLEQVKQWAEKLDALTATAVQDPEGFLGHPVNAFKLMKRLNTEWGEVEDLVLKDMSDGFISNLTIQRQYFPNDEDQNGAAKALLRLQDTYKLDTQTISSGDLPGVNTGLPYKSTLTVEDCFELGKIAYSDADYYHTELWMAQALKQLDEGEESSVEIATALDYLSYSVYQQGELERALEYTKRLLTVDGKKEYLPEKRKYEKLCRGEGLKMTPRRQKHLFCRYFNGNRHPFYTIGPVKQEDEWDRPRIIRYHEIITEQEIEKIKELSKPRLRRATISNPITGVLETAHYRISKRRATVHDPQTGKLTTAQYRVSKSAWLAAYEHPVVDRINQRIEDITGLNVKTAEELQVANYGVGGQYEPHFDFGRKDEPDAFKELGTGNRIATWLFYMSDVAAGGATVFPEVGAAVKPLKGTAVFWYNLFPSGEGDYSTRHAACPVLVGNKWVSNKWIHERGQEFRRPCGLKETD

Target ORF information:

RefSeq Version	NM_001044998.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio prolyl 4-hydroxylase, alpha polypeptide I a (p4ha1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044998.1

ORF Insert Sequence:

ATGATGGGAG TTAGGAGTGT GTGCTGTTTT TTACTCATCT CTCTGCTAAA GTCTTCCTCT 
GCTCACAGCG ACTTCTTCAC TTCCATTGGT CATATGACAG ATCTGTTGTT CACAGAAAAA 
GACCTGGTGA CATCATTGAA AGACTACATT AAAGCAGAGG AGAGCAAACT AGAGCAAGTT 
AAACAGTGGG CAGAGAAGCT TGATGCTTTA ACAGCCACAG CCGTCCAGGA CCCAGAGGGC 
TTTTTGGGTC ATCCTGTCAA TGCCTTTAAA CTCATGAAGA GACTAAACAC AGAATGGGGT 
GAAGTGGAAG ATCTAGTGCT CAAGGACATG TCTGATGGCT TTATCTCAAA CCTGACCATT 
CAGCGCCAGT ACTTCCCCAA TGATGAAGAC CAGAACGGTG CAGCGAAAGC CCTCCTCCGA 
CTGCAGGACA CATATAAACT GGACACACAG ACCATCTCTT CTGGAGACCT GCCTGGTGTT 
AATACAGGTC TGCCATATAA AAGCACCCTG ACGGTAGAGG ACTGTTTTGA ACTAGGAAAA 
ATTGCCTACT CTGACGCAGA CTACTATCAC ACGGAGCTGT GGATGGCCCA GGCGCTCAAG 
CAGCTGGATG AAGGAGAAGA ATCCAGCGTT GAGATTGCCA CCGCTCTGGA TTACCTCAGT 
TACTCTGTCT ACCAACAGGG GGAGCTGGAG AGAGCTCTGG AATATACTAA GAGACTGCTT 
ACTGTGGATG GGAAGAAGGA ATATCTCCCG GAGAAGAGGA AATATGAGAA GCTGTGTAGA 
GGAGAAGGAC TCAAAATGAC TCCTCGGAGA CAGAAGCATT TGTTCTGCCG TTACTTCAAT 
GGTAACAGAC ATCCATTTTA CACAATTGGG CCAGTGAAAC AAGAAGACGA ATGGGACCGT 
CCCCGAATCA TCCGTTATCA TGAGATAATA ACAGAACAAG AAATAGAGAA AATCAAGGAG 
CTCTCCAAAC CAAGGCTGCG CCGAGCCACC ATCTCCAACC CCATTACTGG TGTCCTAGAG 
ACTGCACACT ATCGCATCAG TAAAAGGCGC GCCACCGTTC ATGACCCTCA AACTGGCAAA 
CTCACCACCG CCCAGTACAG AGTCTCCAAG AGTGCGTGGT TGGCGGCCTA TGAGCATCCG 
GTTGTGGATC GCATCAATCA GCGCATTGAA GACATCACAG GCCTCAATGT CAAAACCGCA 
GAAGAGTTAC AGGTGGCGAA TTATGGTGTT GGAGGACAAT ATGAGCCCCA TTTTGACTTT 
GGACGGAAAG ATGAACCAGA TGCCTTTAAA GAGCTGGGCA CAGGGAATCG CATAGCAACC 
TGGCTCTTCT ACATGAGTGA TGTAGCAGCA GGGGGAGCCA CTGTCTTTCC AGAAGTAGGA 
GCTGCAGTAA AGCCACTGAA GGGCACCGCA GTGTTCTGGT ATAATCTGTT TCCCAGTGGA 
GAAGGAGATT ACAGCACGAG ACACGCAGCC TGTCCAGTAC TGGTGGGAAA CAAGTGGGTG 
TCAAATAAAT GGATCCACGA GCGAGGTCAG GAATTCAGGA GACCCTGCGG CCTGAAAGAG 
ACCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

p4ha1a ( XM_009306501.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304776.1 Danio rerio prolyl 4-hydroxylase, alpha polypeptide I a (p4ha1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17646
Clone ID Related Accession (Same CDS sequence)	XM_009306501.3 , XM_009306501.2
Accession Version	XM_009306501.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	prolyl 4-hydroxylase, alpha polypeptide I a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306501.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGAG TTAGGAGTGT GTGCTGTTTT TTACTCATCT CTCTGCTAAA GTCTTCCTCT 
GCTCACAGCG ACTTCTTCAC TTCCATTGGT CATATGACAG ATCTGTTGTT CACAGAAAAA 
GACCTGGTGA CATCATTGAA AGACTACATT AAAGCAGAGG AGAGCAAACT AGAGCAAGTT 
AAACAGTGGG CAGAGAAGCT TGATGCTTTA ACAGCCACAG CCGTCCAGGA CCCAGAGGGC 
TTTTTGGGTC ATCCTGTCAA TGCCTTTAAA CTCATGAAGA GACTAAACAC AGAATGGGGT 
GAAGTGGAAG ATCTAGTGCT CAAGGACATG TCTGATGGCT TTATCTCAAA CCTGACCATT 
CAGCGCCAGT ACTTCCCCAA TGATGAAGAC CAGAACGGTG CAGCGAAAGC CCTCCTCCGA 
CTGCAGGACA CATATAAACT GGACACACAG ACCATCTCTT CTGGAGACCT GCCTGGTGTT 
AATACAGGTC TGCCATATAA AAGCACCCTG ACGGTAGAGG ACTGTTTTGA ACTAGGAAAA 
ATTGCCTACT CTGACGCAGA CTACTATCAC ACGGAGCTGT GGATGGCCCA GGCGCTCAAG 
CAGCTGGATG AAGGAGAAGA ATCCAGCGTT GAGATTGCCA CCGCTCTGGA TTACCTCAGT 
TACTCTGTCT ACCAACAGGG GGAGCTGGAG AGAGCTCTGG AATATACTAA GAGACTGCTT 
ACTGTGGAGC CGGAGCACCA GCGAGCTCTT GGGAACCTCA AATATTTTGA CTACCAGCTG 
GCCAAACAGA AGAAGGCAGA GAAGGAGCAG AGCACCAAGG AAGAGAGCAA GAAGGAGCAA 
GAAACGTCAG ATGGGAAGAA GGAATATCTC CCGGAGAAGA GGAAATATGA GAAGCTGTGT 
AGAGGAGAAG GACTCAAAAT GACTCCTCGG AGACAGAAGC ATTTGTTCTG CCGTTACTTC 
AATGGTAACA GACATCCATT TTACACAATT GGGCCAGTGA AACAAGAAGA CGAATGGGAC 
CGTCCCCGAA TCATCCGTTA TCATGAGATA ATAACAGAAC AAGAAATAGA GAAAATCAAG 
GAGCTCTCCA AACCAAGGCT GCGCCGAGCC ACCATCTCCA ACCCCATTAC TGGTGTCCTA 
GAGACTGCAC ACTATCGCAT CAGTAAAAGT GCGTGGTTGG CGGCCTATGA GCATCCGGTT 
GTGGATCGCA TCAATCAGCG CATTGAAGAC ATCACAGGCC TCAATGTCAA AACCGCAGAA 
GAGTTACAGG TGGCGAATTA TGGTGTTGGA GGACAATATG AGCCCCATTT TGACTTTGGA 
CGGAAAGATG AACCAGATGC CTTTAAAGAG CTGGGCACAG GGAATCGCAT AGCAACCTGG 
CTCTTCTACA TGAGTGATGT AGCAGCAGGG GGAGCCACTG TCTTTCCAGA AGTAGGAGCT 
GCAGTAAAGC CACTGAAGGG CACCGCAGTG TTCTGGTATA ATCTGTTTCC CAGTGGAGAA 
GGAGATTACA GCACGAGACA CGCAGCCTGT CCAGTACTGG TGGGAAACAA GTGGGTGTCA 
AATAAATGGA TCCACGAGCG AGGTCAGGAA TTCAGGAGAC CCTGCGGCCT GAAAGAGACC 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304776.1
CDS	147..1772
Translation	MMGVRSVCCFLLISLLKSSSAHSDFFTSIGHMTDLLFTEKDLVTSLKDYIKAEESKLEQVKQWAEKLDALTATAVQDPEGFLGHPVNAFKLMKRLNTEWGEVEDLVLKDMSDGFISNLTIQRQYFPNDEDQNGAAKALLRLQDTYKLDTQTISSGDLPGVNTGLPYKSTLTVEDCFELGKIAYSDADYYHTELWMAQALKQLDEGEESSVEIATALDYLSYSVYQQGELERALEYTKRLLTVEPEHQRALGNLKYFDYQLAKQKKAEKEQSTKEESKKEQETSDGKKEYLPEKRKYEKLCRGEGLKMTPRRQKHLFCRYFNGNRHPFYTIGPVKQEDEWDRPRIIRYHEIITEQEIEKIKELSKPRLRRATISNPITGVLETAHYRISKSAWLAAYEHPVVDRINQRIEDITGLNVKTAEELQVANYGVGGQYEPHFDFGRKDEPDAFKELGTGNRIATWLFYMSDVAAGGATVFPEVGAAVKPLKGTAVFWYNLFPSGEGDYSTRHAACPVLVGNKWVSNKWIHERGQEFRRPCGLKETD

Target ORF information:

RefSeq Version	XM_009306501.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio prolyl 4-hydroxylase, alpha polypeptide I a (p4ha1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306501.3

ORF Insert Sequence:

ATGATGGGAG TTAGGAGTGT GTGCTGTTTT TTACTCATCT CTCTGCTAAA GTCTTCCTCT 
GCTCACAGCG ACTTCTTCAC TTCCATTGGT CATATGACAG ATCTGTTGTT CACAGAAAAA 
GACCTGGTGA CATCATTGAA AGACTACATT AAAGCAGAGG AGAGCAAACT AGAGCAAGTT 
AAACAGTGGG CAGAGAAGCT TGATGCTTTA ACAGCCACAG CCGTCCAGGA CCCAGAGGGC 
TTTTTGGGTC ATCCTGTCAA TGCCTTTAAA CTCATGAAGA GACTAAACAC AGAATGGGGT 
GAAGTGGAAG ATCTAGTGCT CAAGGACATG TCTGATGGCT TTATCTCAAA CCTGACCATT 
CAGCGCCAGT ACTTCCCCAA TGATGAAGAC CAGAACGGTG CAGCGAAAGC CCTCCTCCGA 
CTGCAGGACA CATATAAACT GGACACACAG ACCATCTCTT CTGGAGACCT GCCTGGTGTT 
AATACAGGTC TGCCATATAA AAGCACCCTG ACGGTAGAGG ACTGTTTTGA ACTAGGAAAA 
ATTGCCTACT CTGACGCAGA CTACTATCAC ACGGAGCTGT GGATGGCCCA GGCGCTCAAG 
CAGCTGGATG AAGGAGAAGA ATCCAGCGTT GAGATTGCCA CCGCTCTGGA TTACCTCAGT 
TACTCTGTCT ACCAACAGGG GGAGCTGGAG AGAGCTCTGG AATATACTAA GAGACTGCTT 
ACTGTGGAGC CGGAGCACCA GCGAGCTCTT GGGAACCTCA AATATTTTGA CTACCAGCTG 
GCCAAACAGA AGAAGGCAGA GAAGGAGCAG AGCACCAAGG AAGAGAGCAA GAAGGAGCAA 
GAAACGTCAG ATGGGAAGAA GGAATATCTC CCGGAGAAGA GGAAATATGA GAAGCTGTGT 
AGAGGAGAAG GACTCAAAAT GACTCCTCGG AGACAGAAGC ATTTGTTCTG CCGTTACTTC 
AATGGTAACA GACATCCATT TTACACAATT GGGCCAGTGA AACAAGAAGA CGAATGGGAC 
CGTCCCCGAA TCATCCGTTA TCATGAGATA ATAACAGAAC AAGAAATAGA GAAAATCAAG 
GAGCTCTCCA AACCAAGGCT GCGCCGAGCC ACCATCTCCA ACCCCATTAC TGGTGTCCTA 
GAGACTGCAC ACTATCGCAT CAGTAAAAGT GCGTGGTTGG CGGCCTATGA GCATCCGGTT 
GTGGATCGCA TCAATCAGCG CATTGAAGAC ATCACAGGCC TCAATGTCAA AACCGCAGAA 
GAGTTACAGG TGGCGAATTA TGGTGTTGGA GGACAATATG AGCCCCATTT TGACTTTGGA 
CGGAAAGATG AACCAGATGC CTTTAAAGAG CTGGGCACAG GGAATCGCAT AGCAACCTGG 
CTCTTCTACA TGAGTGATGT AGCAGCAGGG GGAGCCACTG TCTTTCCAGA AGTAGGAGCT 
GCAGTAAAGC CACTGAAGGG CACCGCAGTG TTCTGGTATA ATCTGTTTCC CAGTGGAGAA 
GGAGATTACA GCACGAGACA CGCAGCCTGT CCAGTACTGG TGGGAAACAA GTGGGTGTCA 
AATAAATGGA TCCACGAGCG AGGTCAGGAA TTCAGGAGAC CCTGCGGCCT GAAAGAGACC 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

p4ha1a ( XM_009306501.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304776.1 Danio rerio prolyl 4-hydroxylase, alpha polypeptide I a (p4ha1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17646
Clone ID Related Accession (Same CDS sequence)	XM_009306501.3 , XM_009306501.2
Accession Version	XM_009306501.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	prolyl 4-hydroxylase, alpha polypeptide I a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306501.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGAG TTAGGAGTGT GTGCTGTTTT TTACTCATCT CTCTGCTAAA GTCTTCCTCT 
GCTCACAGCG ACTTCTTCAC TTCCATTGGT CATATGACAG ATCTGTTGTT CACAGAAAAA 
GACCTGGTGA CATCATTGAA AGACTACATT AAAGCAGAGG AGAGCAAACT AGAGCAAGTT 
AAACAGTGGG CAGAGAAGCT TGATGCTTTA ACAGCCACAG CCGTCCAGGA CCCAGAGGGC 
TTTTTGGGTC ATCCTGTCAA TGCCTTTAAA CTCATGAAGA GACTAAACAC AGAATGGGGT 
GAAGTGGAAG ATCTAGTGCT CAAGGACATG TCTGATGGCT TTATCTCAAA CCTGACCATT 
CAGCGCCAGT ACTTCCCCAA TGATGAAGAC CAGAACGGTG CAGCGAAAGC CCTCCTCCGA 
CTGCAGGACA CATATAAACT GGACACACAG ACCATCTCTT CTGGAGACCT GCCTGGTGTT 
AATACAGGTC TGCCATATAA AAGCACCCTG ACGGTAGAGG ACTGTTTTGA ACTAGGAAAA 
ATTGCCTACT CTGACGCAGA CTACTATCAC ACGGAGCTGT GGATGGCCCA GGCGCTCAAG 
CAGCTGGATG AAGGAGAAGA ATCCAGCGTT GAGATTGCCA CCGCTCTGGA TTACCTCAGT 
TACTCTGTCT ACCAACAGGG GGAGCTGGAG AGAGCTCTGG AATATACTAA GAGACTGCTT 
ACTGTGGAGC CGGAGCACCA GCGAGCTCTT GGGAACCTCA AATATTTTGA CTACCAGCTG 
GCCAAACAGA AGAAGGCAGA GAAGGAGCAG AGCACCAAGG AAGAGAGCAA GAAGGAGCAA 
GAAACGTCAG ATGGGAAGAA GGAATATCTC CCGGAGAAGA GGAAATATGA GAAGCTGTGT 
AGAGGAGAAG GACTCAAAAT GACTCCTCGG AGACAGAAGC ATTTGTTCTG CCGTTACTTC 
AATGGTAACA GACATCCATT TTACACAATT GGGCCAGTGA AACAAGAAGA CGAATGGGAC 
CGTCCCCGAA TCATCCGTTA TCATGAGATA ATAACAGAAC AAGAAATAGA GAAAATCAAG 
GAGCTCTCCA AACCAAGGCT GCGCCGAGCC ACCATCTCCA ACCCCATTAC TGGTGTCCTA 
GAGACTGCAC ACTATCGCAT CAGTAAAAGT GCGTGGTTGG CGGCCTATGA GCATCCGGTT 
GTGGATCGCA TCAATCAGCG CATTGAAGAC ATCACAGGCC TCAATGTCAA AACCGCAGAA 
GAGTTACAGG TGGCGAATTA TGGTGTTGGA GGACAATATG AGCCCCATTT TGACTTTGGA 
CGGAAAGATG AACCAGATGC CTTTAAAGAG CTGGGCACAG GGAATCGCAT AGCAACCTGG 
CTCTTCTACA TGAGTGATGT AGCAGCAGGG GGAGCCACTG TCTTTCCAGA AGTAGGAGCT 
GCAGTAAAGC CACTGAAGGG CACCGCAGTG TTCTGGTATA ATCTGTTTCC CAGTGGAGAA 
GGAGATTACA GCACGAGACA CGCAGCCTGT CCAGTACTGG TGGGAAACAA GTGGGTGTCA 
AATAAATGGA TCCACGAGCG AGGTCAGGAA TTCAGGAGAC CCTGCGGCCT GAAAGAGACC 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304776.1
CDS	146..1771
Translation	MMGVRSVCCFLLISLLKSSSAHSDFFTSIGHMTDLLFTEKDLVTSLKDYIKAEESKLEQVKQWAEKLDALTATAVQDPEGFLGHPVNAFKLMKRLNTEWGEVEDLVLKDMSDGFISNLTIQRQYFPNDEDQNGAAKALLRLQDTYKLDTQTISSGDLPGVNTGLPYKSTLTVEDCFELGKIAYSDADYYHTELWMAQALKQLDEGEESSVEIATALDYLSYSVYQQGELERALEYTKRLLTVEPEHQRALGNLKYFDYQLAKQKKAEKEQSTKEESKKEQETSDGKKEYLPEKRKYEKLCRGEGLKMTPRRQKHLFCRYFNGNRHPFYTIGPVKQEDEWDRPRIIRYHEIITEQEIEKIKELSKPRLRRATISNPITGVLETAHYRISKSAWLAAYEHPVVDRINQRIEDITGLNVKTAEELQVANYGVGGQYEPHFDFGRKDEPDAFKELGTGNRIATWLFYMSDVAAGGATVFPEVGAAVKPLKGTAVFWYNLFPSGEGDYSTRHAACPVLVGNKWVSNKWIHERGQEFRRPCGLKETD

Target ORF information:

RefSeq Version	XM_009306501.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio prolyl 4-hydroxylase, alpha polypeptide I a (p4ha1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306501.2

ORF Insert Sequence:

ATGATGGGAG TTAGGAGTGT GTGCTGTTTT TTACTCATCT CTCTGCTAAA GTCTTCCTCT 
GCTCACAGCG ACTTCTTCAC TTCCATTGGT CATATGACAG ATCTGTTGTT CACAGAAAAA 
GACCTGGTGA CATCATTGAA AGACTACATT AAAGCAGAGG AGAGCAAACT AGAGCAAGTT 
AAACAGTGGG CAGAGAAGCT TGATGCTTTA ACAGCCACAG CCGTCCAGGA CCCAGAGGGC 
TTTTTGGGTC ATCCTGTCAA TGCCTTTAAA CTCATGAAGA GACTAAACAC AGAATGGGGT 
GAAGTGGAAG ATCTAGTGCT CAAGGACATG TCTGATGGCT TTATCTCAAA CCTGACCATT 
CAGCGCCAGT ACTTCCCCAA TGATGAAGAC CAGAACGGTG CAGCGAAAGC CCTCCTCCGA 
CTGCAGGACA CATATAAACT GGACACACAG ACCATCTCTT CTGGAGACCT GCCTGGTGTT 
AATACAGGTC TGCCATATAA AAGCACCCTG ACGGTAGAGG ACTGTTTTGA ACTAGGAAAA 
ATTGCCTACT CTGACGCAGA CTACTATCAC ACGGAGCTGT GGATGGCCCA GGCGCTCAAG 
CAGCTGGATG AAGGAGAAGA ATCCAGCGTT GAGATTGCCA CCGCTCTGGA TTACCTCAGT 
TACTCTGTCT ACCAACAGGG GGAGCTGGAG AGAGCTCTGG AATATACTAA GAGACTGCTT 
ACTGTGGAGC CGGAGCACCA GCGAGCTCTT GGGAACCTCA AATATTTTGA CTACCAGCTG 
GCCAAACAGA AGAAGGCAGA GAAGGAGCAG AGCACCAAGG AAGAGAGCAA GAAGGAGCAA 
GAAACGTCAG ATGGGAAGAA GGAATATCTC CCGGAGAAGA GGAAATATGA GAAGCTGTGT 
AGAGGAGAAG GACTCAAAAT GACTCCTCGG AGACAGAAGC ATTTGTTCTG CCGTTACTTC 
AATGGTAACA GACATCCATT TTACACAATT GGGCCAGTGA AACAAGAAGA CGAATGGGAC 
CGTCCCCGAA TCATCCGTTA TCATGAGATA ATAACAGAAC AAGAAATAGA GAAAATCAAG 
GAGCTCTCCA AACCAAGGCT GCGCCGAGCC ACCATCTCCA ACCCCATTAC TGGTGTCCTA 
GAGACTGCAC ACTATCGCAT CAGTAAAAGT GCGTGGTTGG CGGCCTATGA GCATCCGGTT 
GTGGATCGCA TCAATCAGCG CATTGAAGAC ATCACAGGCC TCAATGTCAA AACCGCAGAA 
GAGTTACAGG TGGCGAATTA TGGTGTTGGA GGACAATATG AGCCCCATTT TGACTTTGGA 
CGGAAAGATG AACCAGATGC CTTTAAAGAG CTGGGCACAG GGAATCGCAT AGCAACCTGG 
CTCTTCTACA TGAGTGATGT AGCAGCAGGG GGAGCCACTG TCTTTCCAGA AGTAGGAGCT 
GCAGTAAAGC CACTGAAGGG CACCGCAGTG TTCTGGTATA ATCTGTTTCC CAGTGGAGAA 
GGAGATTACA GCACGAGACA CGCAGCCTGT CCAGTACTGG TGGGAAACAA GTGGGTGTCA 
AATAAATGGA TCCACGAGCG AGGTCAGGAA TTCAGGAGAC CCTGCGGCCT GAAAGAGACC 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

p4ha1a ( XM_009306502.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304777.1 Danio rerio prolyl 4-hydroxylase, alpha polypeptide I a (p4ha1a), transcript variant X2, mRNA.

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CloneID	ODa17647
Clone ID Related Accession (Same CDS sequence)	XM_009306502.2 , XM_009306502.3
Accession Version	XM_009306502.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	prolyl 4-hydroxylase, alpha polypeptide I a isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306502.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGAG TTAGGAGTGT GTGCTGTTTT TTACTCATCT CTCTGCTAAA GTCTTCCTCT 
GCTCACAGCG ACTTCTTCAC TTCCATTGGT CATATGACAG ATCTGTTGTT CACAGAAAAA 
GACCTGGTGA CATCATTGAA AGACTACATT AAAGCAGAGG AGAGCAAACT AGAGCAAGTT 
AAACAGTGGG CAGAGAAGCT TGATGCTTTA ACAGCCACAG CCGTCCAGGA CCCAGAGGGC 
TTTTTGGGTC ATCCTGTCAA TGCCTTTAAA CTCATGAAGA GACTAAACAC AGAATGGGGT 
GAAGTGGAAG ATCTAGTGCT CAAGGACATG TCTGATGGCT TTATCTCAAA CCTGACCATT 
CAGCGCCAGT ACTTCCCCAA TGATGAAGAC CAGAACGGTG CAGCGAAAGC CCTCCTCCGA 
CTGCAGGACA CATATAAACT GGACACACAG ACCATCTCTT CTGGAGACCT GCCTGGTGTT 
AATACAGGTC TGCCATATAA AAGCACCCTG ACGGTAGAGG ACTGTTTTGA ACTAGGAAAA 
ATTGCCTACT CTGACGCAGA CTACTATCAC ACGGAGCTGT GGATGGCCCA GGCGCTCAAG 
CAGCTGGATG AAGGAGAAGA ATCCAGCGTT GAGATTGCCA CCGCTCTGGA TTACCTCAGT 
TACTCTGTCT ACCAACAGGG GGAGCTGGAG AGAGCTCTGG AATATACTAA GAGACTGCTT 
ACTGTGGAGC CGGAGCACCA GCGAGCTCTT GGGAACCTCA AATATTTTGA CTACCAGCTG 
GCCAAACAGA AGAAGGCAGA GAAGGAGCAG AGCACCAAGG AAGAGAGCAA GAAGGAGCAA 
GAAACGTCAG ATGGGAAGAA GGAATATCTC CCGGAGAAGA GGAAATATGA GAAGCTGTGT 
AGAGGAGAAG GACTCAAAAT GACTCCTCGG AGACAGAAGC ATTTGTTCTG CCGTTACTTC 
AATGGTAACA GACATCCATT TTACACAATT GGGCCAGTGA AACAAGAAGA CGAATGGGAC 
CGTCCCCGAA TCATCCGTTA TCATGAGATA ATAACAGAAC AAGAAATAGA GAAAATCAAG 
GAGCTCTCCA AACCAAGGCT CAGGCGCGCC ACCGTTCATG ACCCTCAAAC TGGCAAACTC 
ACCACCGCCC AGTACAGAGT CTCCAAGAGT GCGTGGTTGG CGGCCTATGA GCATCCGGTT 
GTGGATCGCA TCAATCAGCG CATTGAAGAC ATCACAGGCC TCAATGTCAA AACCGCAGAA 
GAGTTACAGG TGGCGAATTA TGGTGTTGGA GGACAATATG AGCCCCATTT TGACTTTGGA 
CGGAAAGATG AACCAGATGC CTTTAAAGAG CTGGGCACAG GGAATCGCAT AGCAACCTGG 
CTCTTCTACA TGAGTGATGT AGCAGCAGGG GGAGCCACTG TCTTTCCAGA AGTAGGAGCT 
GCAGTAAAGC CACTGAAGGG CACCGCAGTG TTCTGGTATA ATCTGTTTCC CAGTGGAGAA 
GGAGATTACA GCACGAGACA CGCAGCCTGT CCAGTACTGG TGGGAAACAA GTGGGTGTCA 
AATAAATGGA TCCACGAGCG AGGTCAGGAA TTCAGGAGAC CCTGCGGCCT GAAAGAGACC 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304777.1
CDS	146..1771
Translation	MMGVRSVCCFLLISLLKSSSAHSDFFTSIGHMTDLLFTEKDLVTSLKDYIKAEESKLEQVKQWAEKLDALTATAVQDPEGFLGHPVNAFKLMKRLNTEWGEVEDLVLKDMSDGFISNLTIQRQYFPNDEDQNGAAKALLRLQDTYKLDTQTISSGDLPGVNTGLPYKSTLTVEDCFELGKIAYSDADYYHTELWMAQALKQLDEGEESSVEIATALDYLSYSVYQQGELERALEYTKRLLTVEPEHQRALGNLKYFDYQLAKQKKAEKEQSTKEESKKEQETSDGKKEYLPEKRKYEKLCRGEGLKMTPRRQKHLFCRYFNGNRHPFYTIGPVKQEDEWDRPRIIRYHEIITEQEIEKIKELSKPRLRRATVHDPQTGKLTTAQYRVSKSAWLAAYEHPVVDRINQRIEDITGLNVKTAEELQVANYGVGGQYEPHFDFGRKDEPDAFKELGTGNRIATWLFYMSDVAAGGATVFPEVGAAVKPLKGTAVFWYNLFPSGEGDYSTRHAACPVLVGNKWVSNKWIHERGQEFRRPCGLKETD

Target ORF information:

RefSeq Version	XM_009306502.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio prolyl 4-hydroxylase, alpha polypeptide I a (p4ha1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306502.2

ORF Insert Sequence:

ATGATGGGAG TTAGGAGTGT GTGCTGTTTT TTACTCATCT CTCTGCTAAA GTCTTCCTCT 
GCTCACAGCG ACTTCTTCAC TTCCATTGGT CATATGACAG ATCTGTTGTT CACAGAAAAA 
GACCTGGTGA CATCATTGAA AGACTACATT AAAGCAGAGG AGAGCAAACT AGAGCAAGTT 
AAACAGTGGG CAGAGAAGCT TGATGCTTTA ACAGCCACAG CCGTCCAGGA CCCAGAGGGC 
TTTTTGGGTC ATCCTGTCAA TGCCTTTAAA CTCATGAAGA GACTAAACAC AGAATGGGGT 
GAAGTGGAAG ATCTAGTGCT CAAGGACATG TCTGATGGCT TTATCTCAAA CCTGACCATT 
CAGCGCCAGT ACTTCCCCAA TGATGAAGAC CAGAACGGTG CAGCGAAAGC CCTCCTCCGA 
CTGCAGGACA CATATAAACT GGACACACAG ACCATCTCTT CTGGAGACCT GCCTGGTGTT 
AATACAGGTC TGCCATATAA AAGCACCCTG ACGGTAGAGG ACTGTTTTGA ACTAGGAAAA 
ATTGCCTACT CTGACGCAGA CTACTATCAC ACGGAGCTGT GGATGGCCCA GGCGCTCAAG 
CAGCTGGATG AAGGAGAAGA ATCCAGCGTT GAGATTGCCA CCGCTCTGGA TTACCTCAGT 
TACTCTGTCT ACCAACAGGG GGAGCTGGAG AGAGCTCTGG AATATACTAA GAGACTGCTT 
ACTGTGGAGC CGGAGCACCA GCGAGCTCTT GGGAACCTCA AATATTTTGA CTACCAGCTG 
GCCAAACAGA AGAAGGCAGA GAAGGAGCAG AGCACCAAGG AAGAGAGCAA GAAGGAGCAA 
GAAACGTCAG ATGGGAAGAA GGAATATCTC CCGGAGAAGA GGAAATATGA GAAGCTGTGT 
AGAGGAGAAG GACTCAAAAT GACTCCTCGG AGACAGAAGC ATTTGTTCTG CCGTTACTTC 
AATGGTAACA GACATCCATT TTACACAATT GGGCCAGTGA AACAAGAAGA CGAATGGGAC 
CGTCCCCGAA TCATCCGTTA TCATGAGATA ATAACAGAAC AAGAAATAGA GAAAATCAAG 
GAGCTCTCCA AACCAAGGCT CAGGCGCGCC ACCGTTCATG ACCCTCAAAC TGGCAAACTC 
ACCACCGCCC AGTACAGAGT CTCCAAGAGT GCGTGGTTGG CGGCCTATGA GCATCCGGTT 
GTGGATCGCA TCAATCAGCG CATTGAAGAC ATCACAGGCC TCAATGTCAA AACCGCAGAA 
GAGTTACAGG TGGCGAATTA TGGTGTTGGA GGACAATATG AGCCCCATTT TGACTTTGGA 
CGGAAAGATG AACCAGATGC CTTTAAAGAG CTGGGCACAG GGAATCGCAT AGCAACCTGG 
CTCTTCTACA TGAGTGATGT AGCAGCAGGG GGAGCCACTG TCTTTCCAGA AGTAGGAGCT 
GCAGTAAAGC CACTGAAGGG CACCGCAGTG TTCTGGTATA ATCTGTTTCC CAGTGGAGAA 
GGAGATTACA GCACGAGACA CGCAGCCTGT CCAGTACTGG TGGGAAACAA GTGGGTGTCA 
AATAAATGGA TCCACGAGCG AGGTCAGGAA TTCAGGAGAC CCTGCGGCCT GAAAGAGACC 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

p4ha1a ( XM_009306502.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304777.1 Danio rerio prolyl 4-hydroxylase, alpha polypeptide I a (p4ha1a), transcript variant X2, mRNA.

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CloneID	ODa17647
Clone ID Related Accession (Same CDS sequence)	XM_009306502.2 , XM_009306502.3
Accession Version	XM_009306502.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	prolyl 4-hydroxylase, alpha polypeptide I a isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306502.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGAG TTAGGAGTGT GTGCTGTTTT TTACTCATCT CTCTGCTAAA GTCTTCCTCT 
GCTCACAGCG ACTTCTTCAC TTCCATTGGT CATATGACAG ATCTGTTGTT CACAGAAAAA 
GACCTGGTGA CATCATTGAA AGACTACATT AAAGCAGAGG AGAGCAAACT AGAGCAAGTT 
AAACAGTGGG CAGAGAAGCT TGATGCTTTA ACAGCCACAG CCGTCCAGGA CCCAGAGGGC 
TTTTTGGGTC ATCCTGTCAA TGCCTTTAAA CTCATGAAGA GACTAAACAC AGAATGGGGT 
GAAGTGGAAG ATCTAGTGCT CAAGGACATG TCTGATGGCT TTATCTCAAA CCTGACCATT 
CAGCGCCAGT ACTTCCCCAA TGATGAAGAC CAGAACGGTG CAGCGAAAGC CCTCCTCCGA 
CTGCAGGACA CATATAAACT GGACACACAG ACCATCTCTT CTGGAGACCT GCCTGGTGTT 
AATACAGGTC TGCCATATAA AAGCACCCTG ACGGTAGAGG ACTGTTTTGA ACTAGGAAAA 
ATTGCCTACT CTGACGCAGA CTACTATCAC ACGGAGCTGT GGATGGCCCA GGCGCTCAAG 
CAGCTGGATG AAGGAGAAGA ATCCAGCGTT GAGATTGCCA CCGCTCTGGA TTACCTCAGT 
TACTCTGTCT ACCAACAGGG GGAGCTGGAG AGAGCTCTGG AATATACTAA GAGACTGCTT 
ACTGTGGAGC CGGAGCACCA GCGAGCTCTT GGGAACCTCA AATATTTTGA CTACCAGCTG 
GCCAAACAGA AGAAGGCAGA GAAGGAGCAG AGCACCAAGG AAGAGAGCAA GAAGGAGCAA 
GAAACGTCAG ATGGGAAGAA GGAATATCTC CCGGAGAAGA GGAAATATGA GAAGCTGTGT 
AGAGGAGAAG GACTCAAAAT GACTCCTCGG AGACAGAAGC ATTTGTTCTG CCGTTACTTC 
AATGGTAACA GACATCCATT TTACACAATT GGGCCAGTGA AACAAGAAGA CGAATGGGAC 
CGTCCCCGAA TCATCCGTTA TCATGAGATA ATAACAGAAC AAGAAATAGA GAAAATCAAG 
GAGCTCTCCA AACCAAGGCT CAGGCGCGCC ACCGTTCATG ACCCTCAAAC TGGCAAACTC 
ACCACCGCCC AGTACAGAGT CTCCAAGAGT GCGTGGTTGG CGGCCTATGA GCATCCGGTT 
GTGGATCGCA TCAATCAGCG CATTGAAGAC ATCACAGGCC TCAATGTCAA AACCGCAGAA 
GAGTTACAGG TGGCGAATTA TGGTGTTGGA GGACAATATG AGCCCCATTT TGACTTTGGA 
CGGAAAGATG AACCAGATGC CTTTAAAGAG CTGGGCACAG GGAATCGCAT AGCAACCTGG 
CTCTTCTACA TGAGTGATGT AGCAGCAGGG GGAGCCACTG TCTTTCCAGA AGTAGGAGCT 
GCAGTAAAGC CACTGAAGGG CACCGCAGTG TTCTGGTATA ATCTGTTTCC CAGTGGAGAA 
GGAGATTACA GCACGAGACA CGCAGCCTGT CCAGTACTGG TGGGAAACAA GTGGGTGTCA 
AATAAATGGA TCCACGAGCG AGGTCAGGAA TTCAGGAGAC CCTGCGGCCT GAAAGAGACC 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304777.1
CDS	147..1772
Translation	MMGVRSVCCFLLISLLKSSSAHSDFFTSIGHMTDLLFTEKDLVTSLKDYIKAEESKLEQVKQWAEKLDALTATAVQDPEGFLGHPVNAFKLMKRLNTEWGEVEDLVLKDMSDGFISNLTIQRQYFPNDEDQNGAAKALLRLQDTYKLDTQTISSGDLPGVNTGLPYKSTLTVEDCFELGKIAYSDADYYHTELWMAQALKQLDEGEESSVEIATALDYLSYSVYQQGELERALEYTKRLLTVEPEHQRALGNLKYFDYQLAKQKKAEKEQSTKEESKKEQETSDGKKEYLPEKRKYEKLCRGEGLKMTPRRQKHLFCRYFNGNRHPFYTIGPVKQEDEWDRPRIIRYHEIITEQEIEKIKELSKPRLRRATVHDPQTGKLTTAQYRVSKSAWLAAYEHPVVDRINQRIEDITGLNVKTAEELQVANYGVGGQYEPHFDFGRKDEPDAFKELGTGNRIATWLFYMSDVAAGGATVFPEVGAAVKPLKGTAVFWYNLFPSGEGDYSTRHAACPVLVGNKWVSNKWIHERGQEFRRPCGLKETD

Target ORF information:

RefSeq Version	XM_009306502.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio prolyl 4-hydroxylase, alpha polypeptide I a (p4ha1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306502.3

ORF Insert Sequence:

ATGATGGGAG TTAGGAGTGT GTGCTGTTTT TTACTCATCT CTCTGCTAAA GTCTTCCTCT 
GCTCACAGCG ACTTCTTCAC TTCCATTGGT CATATGACAG ATCTGTTGTT CACAGAAAAA 
GACCTGGTGA CATCATTGAA AGACTACATT AAAGCAGAGG AGAGCAAACT AGAGCAAGTT 
AAACAGTGGG CAGAGAAGCT TGATGCTTTA ACAGCCACAG CCGTCCAGGA CCCAGAGGGC 
TTTTTGGGTC ATCCTGTCAA TGCCTTTAAA CTCATGAAGA GACTAAACAC AGAATGGGGT 
GAAGTGGAAG ATCTAGTGCT CAAGGACATG TCTGATGGCT TTATCTCAAA CCTGACCATT 
CAGCGCCAGT ACTTCCCCAA TGATGAAGAC CAGAACGGTG CAGCGAAAGC CCTCCTCCGA 
CTGCAGGACA CATATAAACT GGACACACAG ACCATCTCTT CTGGAGACCT GCCTGGTGTT 
AATACAGGTC TGCCATATAA AAGCACCCTG ACGGTAGAGG ACTGTTTTGA ACTAGGAAAA 
ATTGCCTACT CTGACGCAGA CTACTATCAC ACGGAGCTGT GGATGGCCCA GGCGCTCAAG 
CAGCTGGATG AAGGAGAAGA ATCCAGCGTT GAGATTGCCA CCGCTCTGGA TTACCTCAGT 
TACTCTGTCT ACCAACAGGG GGAGCTGGAG AGAGCTCTGG AATATACTAA GAGACTGCTT 
ACTGTGGAGC CGGAGCACCA GCGAGCTCTT GGGAACCTCA AATATTTTGA CTACCAGCTG 
GCCAAACAGA AGAAGGCAGA GAAGGAGCAG AGCACCAAGG AAGAGAGCAA GAAGGAGCAA 
GAAACGTCAG ATGGGAAGAA GGAATATCTC CCGGAGAAGA GGAAATATGA GAAGCTGTGT 
AGAGGAGAAG GACTCAAAAT GACTCCTCGG AGACAGAAGC ATTTGTTCTG CCGTTACTTC 
AATGGTAACA GACATCCATT TTACACAATT GGGCCAGTGA AACAAGAAGA CGAATGGGAC 
CGTCCCCGAA TCATCCGTTA TCATGAGATA ATAACAGAAC AAGAAATAGA GAAAATCAAG 
GAGCTCTCCA AACCAAGGCT CAGGCGCGCC ACCGTTCATG ACCCTCAAAC TGGCAAACTC 
ACCACCGCCC AGTACAGAGT CTCCAAGAGT GCGTGGTTGG CGGCCTATGA GCATCCGGTT 
GTGGATCGCA TCAATCAGCG CATTGAAGAC ATCACAGGCC TCAATGTCAA AACCGCAGAA 
GAGTTACAGG TGGCGAATTA TGGTGTTGGA GGACAATATG AGCCCCATTT TGACTTTGGA 
CGGAAAGATG AACCAGATGC CTTTAAAGAG CTGGGCACAG GGAATCGCAT AGCAACCTGG 
CTCTTCTACA TGAGTGATGT AGCAGCAGGG GGAGCCACTG TCTTTCCAGA AGTAGGAGCT 
GCAGTAAAGC CACTGAAGGG CACCGCAGTG TTCTGGTATA ATCTGTTTCC CAGTGGAGAA 
GGAGATTACA GCACGAGACA CGCAGCCTGT CCAGTACTGG TGGGAAACAA GTGGGTGTCA 
AATAAATGGA TCCACGAGCG AGGTCAGGAA TTCAGGAGAC CCTGCGGCCT GAAAGAGACC 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Deficiency of a transmembrane prolyl 4-hydroxylase in the zebrafish leads to basement membrane defects and compromised kidney function.
The Journal of biological chemistry285(53)42023-32(2010 Dec)
Hyv?rinen J,Parikka M,Sormunen R,R?met M,Tryggvason K,Kivirikko KI,Myllyharju J,Koivunen P

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p4ha1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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