plcd3b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	plcd3b
Entrez Gene ID	562569
Full Name	phospholipase C, delta 3b
Synonyms	si:dkeyp-94b4.3
General protein information	Preferred Names phospholipase C, delta 3b Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3-A-like DrPLC-delta 3b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005156129.3	XP_005156186.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3-A-like
XM_005156129.4	XP_005156186.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3-A-like

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PLCD3	XP_425839.4
	PLCD3	XP_001115230.1
Caenorhabditis elegans (roundworm)	plc-4	NP_501213.1
Xenopus tropicalis (tropical clawed frog)	plcd3	XP_002935564.2
Pan troglodytes (chimpanzee)	PLCD3	XP_001142960.3
Mus musculus (house mouse)	Plcd3	NP_690026.2
Danio rerio (zebrafish)	plcd3b	XP_005156186.1
Arabidopsis thaliana (thale cress)	PLC1	NP_195565.2
Bos taurus (cattle)	PLCD3	NP_001179957.1
Danio rerio (zebrafish)	plcd3a	NP_001092893.1
Arabidopsis thaliana (thale cress)	PLC1	NP_568881.1
Homo sapiens (human)	PLCD3	NP_588614.1
Canis lupus familiaris (dog)	PLCD3	XP_005625033.1
Rattus norvegicus (Norway rat)	Plcd3	NP_001099315.2

Gene Ontology

The following plcd3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the plcd3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa17720	XM_005156129.4 Latest version!	Danio rerio phospholipase C, delta 3b (plcd3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
ODa17720	XM_005156129.3	Danio rerio phospholipase C, delta 3b (plcd3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

plcd3b ( XM_005156129.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156186.1 Danio rerio phospholipase C, delta 3b (plcd3b), mRNA.

Price & Availability↑

CloneID	ODa17720
Clone ID Related Accession (Same CDS sequence)	XM_005156129.3 , XM_005156129.4
Accession Version	XM_005156129.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3-A-like
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156129.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGAA AGAAGAAGAC TGAAGCCGGG ATGTCCCAAC TTAAACCAGA CAATCCTCTA 
ATAAAAATGG GAGTTCAAGA CGATGAGGAT GTTAAGATGA TGCTACAGGG CTCCTCTGTG 
ACGAAGGTGC GTTCTCCACG CTGGAAGAGG AAACGAATGC TAAAGCTGAT GAATGATGGG 
GTTACAGTCT GGTGTGAATC CAACAAGGCC ACCAGCAGAG CCAAATCTCA GCAGTCATTC 
TCTGTAGCAG AGGTGGAGTG TGTACTGGAA GGCTGTAAGT CTGAGACGTT GAGAAACCAG 
GCCGCCTCAC TCCCAGAAGA ACAGTGTCTC ACCATTGTGT TCAAAGGAGG AAGAAAGAGC 
TTGGATGTCC AGTGTGACAC AACAGAGGAA GCCCAGCACT GGGTCCATGG CATTCGAACC 
CTCCAGGGCA GAGTCAATAA CATGACCCAG AAAGAAAAAC TCGACAATTG GATCTATGGC 
TACCTGAAAC AAGCTGACAT AAATCATGAT GGCAAGATGT CATATGAGGA GGTTCAAGTC 
CTTTTCAAGC TGGTCAACAT AGACCTGAAT GAAGAATATG CTGCCAGCCT TTTTAAGAAA 
AGTGATAAAT CAGCAGATGG ACGGCTTGAA CATGAGGAGA TCGAGCTATT TTGCCGTGAG 
TTGCTACGTC GGCCAGAGTT GGATGCAGTG TTTCAACAGT ACTCTGCAAA CGGTTGTGTC 
CTGTCCACTG TGGATTTGCG GGAATTCCTG AAAGATCAGG GCGAGGACCA CACCCTTGTA 
CATGCGCAGA GCCTCATCCT CACCTATGAG CTCAATGAAT GGGCCCAGAA GAACCAATTC 
ATGACAGCGA ATGGCTTCAC CATGTACATG CTGTCTAAGG AGAATGATGT GTATAACCCA 
GATCACAGAA GAGTATACCA GGACATGAGC CACCCATTAA GTCACTACTT TATCTCCTCA 
TCACACAACA CCTACCTCAC TAAAGACCAG CTCATCAGTG ATAGTAGCGT TCACCCTTAC 
ATAAGAGCTC TAAGTCAAGG CTGTCGCTGT GTGGAGCTGG ACTGCTGGGA TGGAGAAAAG 
GAGCCAGTTA TTTACCATGG ACACACCCTC ACATCCAAAG TGCCATTTAG TGATGTGGTG 
AAGATCATCG CTGAATATGC TTTCACGACT TCTCCATATC CTCTTATTCT GTCTCTGGAG 
AACCACTGCT CTGTGGAGCA ACAGACAATC ATGGCCCAGC AGTTGCGCTC AATTCTGGGG 
GACAAACTGC TCAGCAAACC TCTCAATGAC AAGCCACTTC AATACCTGCC ATCACCAGAG 
GATCTAAAAG GGAAGATTTT GATGAAAGCA AAGAAGATCA AAGTGGAGCA GGACAGCTCC 
AGCTCCGACT CAAGCTCCTC AGATGATGAA TCTAAAGCTG ATTGTAAACC TAAACCAGAG 
AAGAGAGACT GTAAGTTGAG TTCTGAACTG TCTGATCTGG TGGTTTATAC CTCCAGTGTT 
GCCTTTAAAC AGTTTGATCC ATCCGCCAAG AAAGCTCCAA ACGAGATGTC CTCTTTCTCT 
GAGAGTGATG CGCTGAAACT TATCAAAGAA TCAGGAAAAC TGTTTGTACG TCACAACAGT 
GTTCAACTGA GCCGAATCTA TCCATCTGGT CAGAGGGTGC AGTCTTCTAA CTACAACCCT 
CAGGACATGT GGAATGCTGG TTGCCAGATA GTGGCACTGA ACTTCCAAAC CCAATCTGAA 
GAGATGGATC TGAACAGAGG CCGTTTCTTA GCAAACGGAA ATTGCGGCTA TGTGCTAAAA 
CCAAGCTTTA TGTGCCAGCC TGATTCAGAG TTTAACCCAG AAAACACTGG GGGAGGTCCT 
GGCTACAACC CGACACTACT AACTATCAAG GTGATTTCAG CCCAGCAGCT GCCTAAACCT 
GACAAAGAAA AGCTCAGCTC CATTGTGGAC CCTCTTGTTT GGGTCCAAAT TTACGGGGCT 
CCCATAGACA ACAGCACGAA GAAGACACAC CATATTAACA ATAATGGGTT TAACCCTAGA 
TGGGACTGTA CCTTTAAGTT CCAGCTTCAT GTGCCAGAGC TGGTGTTAAT CCGCTTTATG 
GTCGAGGACC ATGACTACAC CACCAGGAAT GATTTTCTTG GCCAGTTTAC ACTTCCGTTC 
CCAAGCTTGC GGACAGGTTA CAGGCACGTG CGCCTTTTGA ATGCAGATGG CTGTAGCATA 
TCGCCCTCCA GTCTCTTCAT TCATGTCAAA ATCACACCTG GATTCAGCAG CCCCACAAGA 
AAAGTAGTCG CACATAGTGC CCGAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156186.1
CDS	536..2845
Translation	MLRKKKTEAGMSQLKPDNPLIKMGVQDDEDVKMMLQGSSVTKVRSPRWKRKRMLKLMNDGVTVWCESNKATSRAKSQQSFSVAEVECVLEGCKSETLRNQAASLPEEQCLTIVFKGGRKSLDVQCDTTEEAQHWVHGIRTLQGRVNNMTQKEKLDNWIYGYLKQADINHDGKMSYEEVQVLFKLVNIDLNEEYAASLFKKSDKSADGRLEHEEIELFCRELLRRPELDAVFQQYSANGCVLSTVDLREFLKDQGEDHTLVHAQSLILTYELNEWAQKNQFMTANGFTMYMLSKENDVYNPDHRRVYQDMSHPLSHYFISSSHNTYLTKDQLISDSSVHPYIRALSQGCRCVELDCWDGEKEPVIYHGHTLTSKVPFSDVVKIIAEYAFTTSPYPLILSLENHCSVEQQTIMAQQLRSILGDKLLSKPLNDKPLQYLPSPEDLKGKILMKAKKIKVEQDSSSSDSSSSDDESKADCKPKPEKRDCKLSSELSDLVVYTSSVAFKQFDPSAKKAPNEMSSFSESDALKLIKESGKLFVRHNSVQLSRIYPSGQRVQSSNYNPQDMWNAGCQIVALNFQTQSEEMDLNRGRFLANGNCGYVLKPSFMCQPDSEFNPENTGGGPGYNPTLLTIKVISAQQLPKPDKEKLSSIVDPLVWVQIYGAPIDNSTKKTHHINNNGFNPRWDCTFKFQLHVPELVLIRFMVEDHDYTTRNDFLGQFTLPFPSLRTGYRHVRLLNADGCSISPSSLFIHVKITPGFSSPTRKVVAHSARE

Target ORF information:

RefSeq Version	XM_005156129.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phospholipase C, delta 3b (plcd3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156129.3

ORF Insert Sequence:

ATGTTGAGAA AGAAGAAGAC TGAAGCCGGG ATGTCCCAAC TTAAACCAGA CAATCCTCTA 
ATAAAAATGG GAGTTCAAGA CGATGAGGAT GTTAAGATGA TGCTACAGGG CTCCTCTGTG 
ACGAAGGTGC GTTCTCCACG CTGGAAGAGG AAACGAATGC TAAAGCTGAT GAATGATGGG 
GTTACAGTCT GGTGTGAATC CAACAAGGCC ACCAGCAGAG CCAAATCTCA GCAGTCATTC 
TCTGTAGCAG AGGTGGAGTG TGTACTGGAA GGCTGTAAGT CTGAGACGTT GAGAAACCAG 
GCCGCCTCAC TCCCAGAAGA ACAGTGTCTC ACCATTGTGT TCAAAGGAGG AAGAAAGAGC 
TTGGATGTCC AGTGTGACAC AACAGAGGAA GCCCAGCACT GGGTCCATGG CATTCGAACC 
CTCCAGGGCA GAGTCAATAA CATGACCCAG AAAGAAAAAC TCGACAATTG GATCTATGGC 
TACCTGAAAC AAGCTGACAT AAATCATGAT GGCAAGATGT CATATGAGGA GGTTCAAGTC 
CTTTTCAAGC TGGTCAACAT AGACCTGAAT GAAGAATATG CTGCCAGCCT TTTTAAGAAA 
AGTGATAAAT CAGCAGATGG ACGGCTTGAA CATGAGGAGA TCGAGCTATT TTGCCGTGAG 
TTGCTACGTC GGCCAGAGTT GGATGCAGTG TTTCAACAGT ACTCTGCAAA CGGTTGTGTC 
CTGTCCACTG TGGATTTGCG GGAATTCCTG AAAGATCAGG GCGAGGACCA CACCCTTGTA 
CATGCGCAGA GCCTCATCCT CACCTATGAG CTCAATGAAT GGGCCCAGAA GAACCAATTC 
ATGACAGCGA ATGGCTTCAC CATGTACATG CTGTCTAAGG AGAATGATGT GTATAACCCA 
GATCACAGAA GAGTATACCA GGACATGAGC CACCCATTAA GTCACTACTT TATCTCCTCA 
TCACACAACA CCTACCTCAC TAAAGACCAG CTCATCAGTG ATAGTAGCGT TCACCCTTAC 
ATAAGAGCTC TAAGTCAAGG CTGTCGCTGT GTGGAGCTGG ACTGCTGGGA TGGAGAAAAG 
GAGCCAGTTA TTTACCATGG ACACACCCTC ACATCCAAAG TGCCATTTAG TGATGTGGTG 
AAGATCATCG CTGAATATGC TTTCACGACT TCTCCATATC CTCTTATTCT GTCTCTGGAG 
AACCACTGCT CTGTGGAGCA ACAGACAATC ATGGCCCAGC AGTTGCGCTC AATTCTGGGG 
GACAAACTGC TCAGCAAACC TCTCAATGAC AAGCCACTTC AATACCTGCC ATCACCAGAG 
GATCTAAAAG GGAAGATTTT GATGAAAGCA AAGAAGATCA AAGTGGAGCA GGACAGCTCC 
AGCTCCGACT CAAGCTCCTC AGATGATGAA TCTAAAGCTG ATTGTAAACC TAAACCAGAG 
AAGAGAGACT GTAAGTTGAG TTCTGAACTG TCTGATCTGG TGGTTTATAC CTCCAGTGTT 
GCCTTTAAAC AGTTTGATCC ATCCGCCAAG AAAGCTCCAA ACGAGATGTC CTCTTTCTCT 
GAGAGTGATG CGCTGAAACT TATCAAAGAA TCAGGAAAAC TGTTTGTACG TCACAACAGT 
GTTCAACTGA GCCGAATCTA TCCATCTGGT CAGAGGGTGC AGTCTTCTAA CTACAACCCT 
CAGGACATGT GGAATGCTGG TTGCCAGATA GTGGCACTGA ACTTCCAAAC CCAATCTGAA 
GAGATGGATC TGAACAGAGG CCGTTTCTTA GCAAACGGAA ATTGCGGCTA TGTGCTAAAA 
CCAAGCTTTA TGTGCCAGCC TGATTCAGAG TTTAACCCAG AAAACACTGG GGGAGGTCCT 
GGCTACAACC CGACACTACT AACTATCAAG GTGATTTCAG CCCAGCAGCT GCCTAAACCT 
GACAAAGAAA AGCTCAGCTC CATTGTGGAC CCTCTTGTTT GGGTCCAAAT TTACGGGGCT 
CCCATAGACA ACAGCACGAA GAAGACACAC CATATTAACA ATAATGGGTT TAACCCTAGA 
TGGGACTGTA CCTTTAAGTT CCAGCTTCAT GTGCCAGAGC TGGTGTTAAT CCGCTTTATG 
GTCGAGGACC ATGACTACAC CACCAGGAAT GATTTTCTTG GCCAGTTTAC ACTTCCGTTC 
CCAAGCTTGC GGACAGGTTA CAGGCACGTG CGCCTTTTGA ATGCAGATGG CTGTAGCATA 
TCGCCCTCCA GTCTCTTCAT TCATGTCAAA ATCACACCTG GATTCAGCAG CCCCACAAGA 
AAAGTAGTCG CACATAGTGC CCGAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcd3b ( XM_005156129.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156186.1 Danio rerio phospholipase C, delta 3b (plcd3b), mRNA.

Price & Availability↑

CloneID	ODa17720
Clone ID Related Accession (Same CDS sequence)	XM_005156129.3 , XM_005156129.4
Accession Version	XM_005156129.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3-A-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156129.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGAA AGAAGAAGAC TGAAGCCGGG ATGTCCCAAC TTAAACCAGA CAATCCTCTA 
ATAAAAATGG GAGTTCAAGA CGATGAGGAT GTTAAGATGA TGCTACAGGG CTCCTCTGTG 
ACGAAGGTGC GTTCTCCACG CTGGAAGAGG AAACGAATGC TAAAGCTGAT GAATGATGGG 
GTTACAGTCT GGTGTGAATC CAACAAGGCC ACCAGCAGAG CCAAATCTCA GCAGTCATTC 
TCTGTAGCAG AGGTGGAGTG TGTACTGGAA GGCTGTAAGT CTGAGACGTT GAGAAACCAG 
GCCGCCTCAC TCCCAGAAGA ACAGTGTCTC ACCATTGTGT TCAAAGGAGG AAGAAAGAGC 
TTGGATGTCC AGTGTGACAC AACAGAGGAA GCCCAGCACT GGGTCCATGG CATTCGAACC 
CTCCAGGGCA GAGTCAATAA CATGACCCAG AAAGAAAAAC TCGACAATTG GATCTATGGC 
TACCTGAAAC AAGCTGACAT AAATCATGAT GGCAAGATGT CATATGAGGA GGTTCAAGTC 
CTTTTCAAGC TGGTCAACAT AGACCTGAAT GAAGAATATG CTGCCAGCCT TTTTAAGAAA 
AGTGATAAAT CAGCAGATGG ACGGCTTGAA CATGAGGAGA TCGAGCTATT TTGCCGTGAG 
TTGCTACGTC GGCCAGAGTT GGATGCAGTG TTTCAACAGT ACTCTGCAAA CGGTTGTGTC 
CTGTCCACTG TGGATTTGCG GGAATTCCTG AAAGATCAGG GCGAGGACCA CACCCTTGTA 
CATGCGCAGA GCCTCATCCT CACCTATGAG CTCAATGAAT GGGCCCAGAA GAACCAATTC 
ATGACAGCGA ATGGCTTCAC CATGTACATG CTGTCTAAGG AGAATGATGT GTATAACCCA 
GATCACAGAA GAGTATACCA GGACATGAGC CACCCATTAA GTCACTACTT TATCTCCTCA 
TCACACAACA CCTACCTCAC TAAAGACCAG CTCATCAGTG ATAGTAGCGT TCACCCTTAC 
ATAAGAGCTC TAAGTCAAGG CTGTCGCTGT GTGGAGCTGG ACTGCTGGGA TGGAGAAAAG 
GAGCCAGTTA TTTACCATGG ACACACCCTC ACATCCAAAG TGCCATTTAG TGATGTGGTG 
AAGATCATCG CTGAATATGC TTTCACGACT TCTCCATATC CTCTTATTCT GTCTCTGGAG 
AACCACTGCT CTGTGGAGCA ACAGACAATC ATGGCCCAGC AGTTGCGCTC AATTCTGGGG 
GACAAACTGC TCAGCAAACC TCTCAATGAC AAGCCACTTC AATACCTGCC ATCACCAGAG 
GATCTAAAAG GGAAGATTTT GATGAAAGCA AAGAAGATCA AAGTGGAGCA GGACAGCTCC 
AGCTCCGACT CAAGCTCCTC AGATGATGAA TCTAAAGCTG ATTGTAAACC TAAACCAGAG 
AAGAGAGACT GTAAGTTGAG TTCTGAACTG TCTGATCTGG TGGTTTATAC CTCCAGTGTT 
GCCTTTAAAC AGTTTGATCC ATCCGCCAAG AAAGCTCCAA ACGAGATGTC CTCTTTCTCT 
GAGAGTGATG CGCTGAAACT TATCAAAGAA TCAGGAAAAC TGTTTGTACG TCACAACAGT 
GTTCAACTGA GCCGAATCTA TCCATCTGGT CAGAGGGTGC AGTCTTCTAA CTACAACCCT 
CAGGACATGT GGAATGCTGG TTGCCAGATA GTGGCACTGA ACTTCCAAAC CCAATCTGAA 
GAGATGGATC TGAACAGAGG CCGTTTCTTA GCAAACGGAA ATTGCGGCTA TGTGCTAAAA 
CCAAGCTTTA TGTGCCAGCC TGATTCAGAG TTTAACCCAG AAAACACTGG GGGAGGTCCT 
GGCTACAACC CGACACTACT AACTATCAAG GTGATTTCAG CCCAGCAGCT GCCTAAACCT 
GACAAAGAAA AGCTCAGCTC CATTGTGGAC CCTCTTGTTT GGGTCCAAAT TTACGGGGCT 
CCCATAGACA ACAGCACGAA GAAGACACAC CATATTAACA ATAATGGGTT TAACCCTAGA 
TGGGACTGTA CCTTTAAGTT CCAGCTTCAT GTGCCAGAGC TGGTGTTAAT CCGCTTTATG 
GTCGAGGACC ATGACTACAC CACCAGGAAT GATTTTCTTG GCCAGTTTAC ACTTCCGTTC 
CCAAGCTTGC GGACAGGTTA CAGGCACGTG CGCCTTTTGA ATGCAGATGG CTGTAGCATA 
TCGCCCTCCA GTCTCTTCAT TCATGTCAAA ATCACACCTG GATTCAGCAG CCCCACAAGA 
AAAGTAGTCG CACATAGTGC CCGAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156186.1
CDS	539..2848
Translation	MLRKKKTEAGMSQLKPDNPLIKMGVQDDEDVKMMLQGSSVTKVRSPRWKRKRMLKLMNDGVTVWCESNKATSRAKSQQSFSVAEVECVLEGCKSETLRNQAASLPEEQCLTIVFKGGRKSLDVQCDTTEEAQHWVHGIRTLQGRVNNMTQKEKLDNWIYGYLKQADINHDGKMSYEEVQVLFKLVNIDLNEEYAASLFKKSDKSADGRLEHEEIELFCRELLRRPELDAVFQQYSANGCVLSTVDLREFLKDQGEDHTLVHAQSLILTYELNEWAQKNQFMTANGFTMYMLSKENDVYNPDHRRVYQDMSHPLSHYFISSSHNTYLTKDQLISDSSVHPYIRALSQGCRCVELDCWDGEKEPVIYHGHTLTSKVPFSDVVKIIAEYAFTTSPYPLILSLENHCSVEQQTIMAQQLRSILGDKLLSKPLNDKPLQYLPSPEDLKGKILMKAKKIKVEQDSSSSDSSSSDDESKADCKPKPEKRDCKLSSELSDLVVYTSSVAFKQFDPSAKKAPNEMSSFSESDALKLIKESGKLFVRHNSVQLSRIYPSGQRVQSSNYNPQDMWNAGCQIVALNFQTQSEEMDLNRGRFLANGNCGYVLKPSFMCQPDSEFNPENTGGGPGYNPTLLTIKVISAQQLPKPDKEKLSSIVDPLVWVQIYGAPIDNSTKKTHHINNNGFNPRWDCTFKFQLHVPELVLIRFMVEDHDYTTRNDFLGQFTLPFPSLRTGYRHVRLLNADGCSISPSSLFIHVKITPGFSSPTRKVVAHSARE

Target ORF information:

RefSeq Version	XM_005156129.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phospholipase C, delta 3b (plcd3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156129.4

ORF Insert Sequence:

ATGTTGAGAA AGAAGAAGAC TGAAGCCGGG ATGTCCCAAC TTAAACCAGA CAATCCTCTA 
ATAAAAATGG GAGTTCAAGA CGATGAGGAT GTTAAGATGA TGCTACAGGG CTCCTCTGTG 
ACGAAGGTGC GTTCTCCACG CTGGAAGAGG AAACGAATGC TAAAGCTGAT GAATGATGGG 
GTTACAGTCT GGTGTGAATC CAACAAGGCC ACCAGCAGAG CCAAATCTCA GCAGTCATTC 
TCTGTAGCAG AGGTGGAGTG TGTACTGGAA GGCTGTAAGT CTGAGACGTT GAGAAACCAG 
GCCGCCTCAC TCCCAGAAGA ACAGTGTCTC ACCATTGTGT TCAAAGGAGG AAGAAAGAGC 
TTGGATGTCC AGTGTGACAC AACAGAGGAA GCCCAGCACT GGGTCCATGG CATTCGAACC 
CTCCAGGGCA GAGTCAATAA CATGACCCAG AAAGAAAAAC TCGACAATTG GATCTATGGC 
TACCTGAAAC AAGCTGACAT AAATCATGAT GGCAAGATGT CATATGAGGA GGTTCAAGTC 
CTTTTCAAGC TGGTCAACAT AGACCTGAAT GAAGAATATG CTGCCAGCCT TTTTAAGAAA 
AGTGATAAAT CAGCAGATGG ACGGCTTGAA CATGAGGAGA TCGAGCTATT TTGCCGTGAG 
TTGCTACGTC GGCCAGAGTT GGATGCAGTG TTTCAACAGT ACTCTGCAAA CGGTTGTGTC 
CTGTCCACTG TGGATTTGCG GGAATTCCTG AAAGATCAGG GCGAGGACCA CACCCTTGTA 
CATGCGCAGA GCCTCATCCT CACCTATGAG CTCAATGAAT GGGCCCAGAA GAACCAATTC 
ATGACAGCGA ATGGCTTCAC CATGTACATG CTGTCTAAGG AGAATGATGT GTATAACCCA 
GATCACAGAA GAGTATACCA GGACATGAGC CACCCATTAA GTCACTACTT TATCTCCTCA 
TCACACAACA CCTACCTCAC TAAAGACCAG CTCATCAGTG ATAGTAGCGT TCACCCTTAC 
ATAAGAGCTC TAAGTCAAGG CTGTCGCTGT GTGGAGCTGG ACTGCTGGGA TGGAGAAAAG 
GAGCCAGTTA TTTACCATGG ACACACCCTC ACATCCAAAG TGCCATTTAG TGATGTGGTG 
AAGATCATCG CTGAATATGC TTTCACGACT TCTCCATATC CTCTTATTCT GTCTCTGGAG 
AACCACTGCT CTGTGGAGCA ACAGACAATC ATGGCCCAGC AGTTGCGCTC AATTCTGGGG 
GACAAACTGC TCAGCAAACC TCTCAATGAC AAGCCACTTC AATACCTGCC ATCACCAGAG 
GATCTAAAAG GGAAGATTTT GATGAAAGCA AAGAAGATCA AAGTGGAGCA GGACAGCTCC 
AGCTCCGACT CAAGCTCCTC AGATGATGAA TCTAAAGCTG ATTGTAAACC TAAACCAGAG 
AAGAGAGACT GTAAGTTGAG TTCTGAACTG TCTGATCTGG TGGTTTATAC CTCCAGTGTT 
GCCTTTAAAC AGTTTGATCC ATCCGCCAAG AAAGCTCCAA ACGAGATGTC CTCTTTCTCT 
GAGAGTGATG CGCTGAAACT TATCAAAGAA TCAGGAAAAC TGTTTGTACG TCACAACAGT 
GTTCAACTGA GCCGAATCTA TCCATCTGGT CAGAGGGTGC AGTCTTCTAA CTACAACCCT 
CAGGACATGT GGAATGCTGG TTGCCAGATA GTGGCACTGA ACTTCCAAAC CCAATCTGAA 
GAGATGGATC TGAACAGAGG CCGTTTCTTA GCAAACGGAA ATTGCGGCTA TGTGCTAAAA 
CCAAGCTTTA TGTGCCAGCC TGATTCAGAG TTTAACCCAG AAAACACTGG GGGAGGTCCT 
GGCTACAACC CGACACTACT AACTATCAAG GTGATTTCAG CCCAGCAGCT GCCTAAACCT 
GACAAAGAAA AGCTCAGCTC CATTGTGGAC CCTCTTGTTT GGGTCCAAAT TTACGGGGCT 
CCCATAGACA ACAGCACGAA GAAGACACAC CATATTAACA ATAATGGGTT TAACCCTAGA 
TGGGACTGTA CCTTTAAGTT CCAGCTTCAT GTGCCAGAGC TGGTGTTAAT CCGCTTTATG 
GTCGAGGACC ATGACTACAC CACCAGGAAT GATTTTCTTG GCCAGTTTAC ACTTCCGTTC 
CCAAGCTTGC GGACAGGTTA CAGGCACGTG CGCCTTTTGA ATGCAGATGG CTGTAGCATA 
TCGCCCTCCA GTCTCTTCAT TCATGTCAAA ATCACACCTG GATTCAGCAG CCCCACAAGA 
AAAGTAGTCG CACATAGTGC CCGAGAATAA