ezrb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ezrb
Entrez Gene ID	561589
Full Name	ezrin b
Synonyms	ezr,vil2,wu:fc05c03,wu:fj31d06
General protein information	Preferred Names ezrin b Names villin 2 cb567 ezrin2 villin 2 (ezrin)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005160437.4	XP_005160494.1	villin 2 isoform X1
XM_005160437.3	XP_005160494.1	villin 2 isoform X1
NM_001030285.2	NP_001025456.1	villin 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	EZR	XP_541173.2
	EZR	XP_002803901.1
Pan troglodytes (chimpanzee)	EZR	XP_003311612.1
Xenopus tropicalis (tropical clawed frog)	ezr	NP_001025629.1
Mus musculus (house mouse)	Ezr	NP_033536.2
Rattus norvegicus (Norway rat)	Ezr	NP_062230.1
Gallus gallus (chicken)	EZR	NP_990216.1
Danio rerio (zebrafish)	ezra	NP_001018326.1
Homo sapiens (human)	EZR	NP_003370.2
Bos taurus (cattle)	EZR	NP_776642.1
Danio rerio (zebrafish)	ezrb	NP_001025456.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

The Joubert Syndrome Protein Inpp5e Controls Ciliogenesis by Regulating Phosphoinositides at the Apical Membrane.
Xu W, Jin M, Hu R, Wang H, Zhang F, Yuan S, Cao Y
Journal of the American Society of Nephrology : JASN28(1)118-129(2017 Jan)

Steering cell migration by alternating blebs and actin-rich protrusions.
Diz-Mu?oz A, Romanczuk P, Yu W, Bergert M, Ivanovitch K, Salbreux G, Heisenberg CP, Paluch EK
BMC biology1474(2016 Sep)

Identification of Novel Ezrin Inhibitors Targeting Metastatic Osteosarcoma by Screening Open Access Malaria Box.
?elik H, Hong SH, Col?n-L?pez DD, Han J, Kont YS, Minas TZ, Swift M, Paige M, Glasgow E, Toretsky JA, Bosch J, ?ren A
Molecular cancer therapeutics14(11)2497-507(2015 Nov)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ezrb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ezrb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00066	NM_001030285.2 Latest version!	Danio rerio ezrin b (ezrb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa00066	XM_005160437.4 Latest version!	Danio rerio ezrin b (ezrb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa00066	XM_005160437.3	Danio rerio ezrin b (ezrb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ezrb ( NM_001030285.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001025456.1 Danio rerio ezrin b (ezrb), mRNA.

Price & Availability↑

CloneID	ODa00066
Clone ID Related Accession (Same CDS sequence)	XM_005160437.4 , XM_005160437.3 , NM_001030285.2
Accession Version	NM_001030285.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	villin 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX248246.11. On Sep 24, 2006 this sequence version replaced NM_001030285.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC139506.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476807 [ECO:0000348] ##Evidence-Data-END##

ATGCCTAAAC CAGTAAATGT TCGCGTCACT ACAATGGACG CTGAGCTGGA GTTTGCCGTT 
CAGCCCAGCA CAACTGGCAA GCAGCTGTTT GATCAGGTGG TGAAGACCAT CGGGTTACGT 
GAAATATGGT ACTTTGGGCT TCAATATATG GACAGCAAAG GCTACCTTAC ATGGCTGAAA 
CTTGACAAAA AGGTGTCTTC TCAGGACGTG AAGAAAGAGA ATCCGCTGCA GTTTAAGTTT 
CGTGCTAAAT TCTTCCCAGA AGACGTAGCG GATGAGCTGA TCCAGGACAT CACTCAAAAA 
CTCTTCTTCA TGCAAGTGAA AGATGGCATT CTGAGTGACG AGATCTATTG TCCTCCAGAG 
ACTGCCGTGC TCTTGGCGTC CTATTCAGTG CAGGCCAAGT TTGGGGATTT CAGCAAGGAG 
TTGCACAGGC CTGGATACCT GACATCAGAA CGCCTGCTGC CCCAACGGGT TCTGGATCAG 
CACAAGTTGT CACGGGAACA GTGGGAGGAG AGGATTCAGG TGTGGCACGA GGAACATCGG 
GGGATGCTCA GAGAGGACGC AATGCTGGAG TATTTGAAAA TCGCTCAGGA TCTGGAAATG 
TACGGCGTGA ACTATTTTGA CATCAAGAAC AAGAAGGGAA CAGAGCTGTG GCTGGGTGTG 
GATGCCCTGG GCCTAAATAT CTATGAGAAA GACGACAAAC TCACTCCAAA AATTGGATTC 
CCCTGGAGCG AAATCCGAAA CATTTCCTTC AACGATAAGA AGTTTGTCAT CAAGCCCATA 
GATAAAAAGG CTCCAGATTT CGTGTTCTAC GCTCCACGTT TGCGCATTAA TAAGCGCATC 
CTTCAGCTGT GCATGGGGAA CCACGAGCTG TACATGCGCC GCAGGAAGCC GGATACCATC 
GAGGTGCAGC AGATGAAAGC TCAGGCTCGA GAGGAGAAAC AACAGAAACA GATGGAGAGA 
GCTCAGCTGG AGAACGAGAA GAAGAGACGA GAGGCCATCG AGAGAGAGAA GGAGCAAATG 
GAAAGAGAGA AAAAGGAGCT GATGATGCAA CTCTACCAGT TCGAGGAGAG GACCAAAAAA 
GTAGAGAATG AATTGCAGGA GCAGATGCAG AGGGCCATGC AGCTGCAGCA CGAACGGAGA 
CTGGCTGATG AAGAAGCCGC TAGACTGGAG GCCGAGCGAC AGGCAGCACT GCTCGCCAAG 
GAGGAGCTGG CCCGTCAAGC ACAAGATCAG CTCAAGAGTC AGGAGCAGCT GGCTGCTGAG 
CTGGCAGAAT ACACTGCAAA AATTTCACTC CTTGAAGAAG CCAAAAAACG CAAGGAGGAA 
GAAGCTCAGA CATGGCAGAA CAAAGCACAA TTAGCTCAAG ATGACCTGAT AAAGACGCGT 
CAGGAGCTTC ACAATGTGAT GACGGCGCCA CCTCTTCCTC CTCCACCACC TGCATACGAC 
CATGATGAAA ATGATCTCGA TGATGGAGAG GAGAGCAATG GCAGCTACAC CGCAGACCTG 
CAGACGGGAG GATTCAATGA CCACCGCCTG GAGGAAGAGC GAATCACTGA GGCCGAGAAG 
AACGAACGAG TTCAGAAACA ACTGCTGGCT CTGACCTCAG AATTGGCCCA AGCTCGAGAC 
GACACCAAGA AGACTCAGAA CGACCTGCTG CACACAGAGA ACGTGCGCGC TGGCCGAGAC 
AAGTACAAAA CTCTCCGGCA GATCCGCCAG GGTAACACCA AACAGAGAAT CGACGAGTTC 
GAGGCCTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025456.1
CDS	106..1857
Translation	MPKPVNVRVTTMDAELEFAVQPSTTGKQLFDQVVKTIGLREIWYFGLQYMDSKGYLTWLKLDKKVSSQDVKKENPLQFKFRAKFFPEDVADELIQDITQKLFFMQVKDGILSDEIYCPPETAVLLASYSVQAKFGDFSKELHRPGYLTSERLLPQRVLDQHKLSREQWEERIQVWHEEHRGMLREDAMLEYLKIAQDLEMYGVNYFDIKNKKGTELWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKQQKQMERAQLENEKKRREAIEREKEQMEREKKELMMQLYQFEERTKKVENELQEQMQRAMQLQHERRLADEEAARLEAERQAALLAKEELARQAQDQLKSQEQLAAELAEYTAKISLLEEAKKRKEEEAQTWQNKAQLAQDDLIKTRQELHNVMTAPPLPPPPPAYDHDENDLDDGEESNGSYTADLQTGGFNDHRLEEERITEAEKNERVQKQLLALTSELAQARDDTKKTQNDLLHTENVRAGRDKYKTLRQIRQGNTKQRIDEFEAL

Target ORF information:

RefSeq Version	NM_001030285.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ezrin b (ezrb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030285.2

ORF Insert Sequence:

ATGCCTAAAC CAGTAAATGT TCGCGTCACT ACAATGGACG CTGAGCTGGA GTTTGCCGTT 
CAGCCCAGCA CAACTGGCAA GCAGCTGTTT GATCAGGTGG TGAAGACCAT CGGGTTACGT 
GAAATATGGT ACTTTGGGCT TCAATATATG GACAGCAAAG GCTACCTTAC ATGGCTGAAA 
CTTGACAAAA AGGTGTCTTC TCAGGACGTG AAGAAAGAGA ATCCGCTGCA GTTTAAGTTT 
CGTGCTAAAT TCTTCCCAGA AGACGTAGCG GATGAGCTGA TCCAGGACAT CACTCAAAAA 
CTCTTCTTCA TGCAAGTGAA AGATGGCATT CTGAGTGACG AGATCTATTG TCCTCCAGAG 
ACTGCCGTGC TCTTGGCGTC CTATTCAGTG CAGGCCAAGT TTGGGGATTT CAGCAAGGAG 
TTGCACAGGC CTGGATACCT GACATCAGAA CGCCTGCTGC CCCAACGGGT TCTGGATCAG 
CACAAGTTGT CACGGGAACA GTGGGAGGAG AGGATTCAGG TGTGGCACGA GGAACATCGG 
GGGATGCTCA GAGAGGACGC AATGCTGGAG TATTTGAAAA TCGCTCAGGA TCTGGAAATG 
TACGGCGTGA ACTATTTTGA CATCAAGAAC AAGAAGGGAA CAGAGCTGTG GCTGGGTGTG 
GATGCCCTGG GCCTAAATAT CTATGAGAAA GACGACAAAC TCACTCCAAA AATTGGATTC 
CCCTGGAGCG AAATCCGAAA CATTTCCTTC AACGATAAGA AGTTTGTCAT CAAGCCCATA 
GATAAAAAGG CTCCAGATTT CGTGTTCTAC GCTCCACGTT TGCGCATTAA TAAGCGCATC 
CTTCAGCTGT GCATGGGGAA CCACGAGCTG TACATGCGCC GCAGGAAGCC GGATACCATC 
GAGGTGCAGC AGATGAAAGC TCAGGCTCGA GAGGAGAAAC AACAGAAACA GATGGAGAGA 
GCTCAGCTGG AGAACGAGAA GAAGAGACGA GAGGCCATCG AGAGAGAGAA GGAGCAAATG 
GAAAGAGAGA AAAAGGAGCT GATGATGCAA CTCTACCAGT TCGAGGAGAG GACCAAAAAA 
GTAGAGAATG AATTGCAGGA GCAGATGCAG AGGGCCATGC AGCTGCAGCA CGAACGGAGA 
CTGGCTGATG AAGAAGCCGC TAGACTGGAG GCCGAGCGAC AGGCAGCACT GCTCGCCAAG 
GAGGAGCTGG CCCGTCAAGC ACAAGATCAG CTCAAGAGTC AGGAGCAGCT GGCTGCTGAG 
CTGGCAGAAT ACACTGCAAA AATTTCACTC CTTGAAGAAG CCAAAAAACG CAAGGAGGAA 
GAAGCTCAGA CATGGCAGAA CAAAGCACAA TTAGCTCAAG ATGACCTGAT AAAGACGCGT 
CAGGAGCTTC ACAATGTGAT GACGGCGCCA CCTCTTCCTC CTCCACCACC TGCATACGAC 
CATGATGAAA ATGATCTCGA TGATGGAGAG GAGAGCAATG GCAGCTACAC CGCAGACCTG 
CAGACGGGAG GATTCAATGA CCACCGCCTG GAGGAAGAGC GAATCACTGA GGCCGAGAAG 
AACGAACGAG TTCAGAAACA ACTGCTGGCT CTGACCTCAG AATTGGCCCA AGCTCGAGAC 
GACACCAAGA AGACTCAGAA CGACCTGCTG CACACAGAGA ACGTGCGCGC TGGCCGAGAC 
AAGTACAAAA CTCTCCGGCA GATCCGCCAG GGTAACACCA AACAGAGAAT CGACGAGTTC 
GAGGCCTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ezrb ( XM_005160437.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160494.1 Danio rerio ezrin b (ezrb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa00066
Clone ID Related Accession (Same CDS sequence)	XM_005160437.4 , XM_005160437.3 , NM_001030285.2
Accession Version	XM_005160437.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	villin 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160437.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTAAAC CAGTAAATGT TCGCGTCACT ACAATGGACG CTGAGCTGGA GTTTGCCGTT 
CAGCCCAGCA CAACTGGCAA GCAGCTGTTT GATCAGGTGG TGAAGACCAT CGGGTTACGT 
GAAATATGGT ACTTTGGGCT TCAATATATG GACAGCAAAG GCTACCTTAC ATGGCTGAAA 
CTTGACAAAA AGGTGTCTTC TCAGGACGTG AAGAAAGAGA ATCCGCTGCA GTTTAAGTTT 
CGTGCTAAAT TCTTCCCAGA AGACGTAGCG GATGAGCTGA TCCAGGACAT CACTCAAAAA 
CTCTTCTTCA TGCAAGTGAA AGATGGCATT CTGAGTGACG AGATCTATTG TCCTCCAGAG 
ACTGCCGTGC TCTTGGCGTC CTATTCAGTG CAGGCCAAGT TTGGGGATTT CAGCAAGGAG 
TTGCACAGGC CTGGATACCT GACATCAGAA CGCCTGCTGC CCCAACGGGT TCTGGATCAG 
CACAAGTTGT CACGGGAACA GTGGGAGGAG AGGATTCAGG TGTGGCACGA GGAACATCGG 
GGGATGCTCA GAGAGGACGC AATGCTGGAG TATTTGAAAA TCGCTCAGGA TCTGGAAATG 
TACGGCGTGA ACTATTTTGA CATCAAGAAC AAGAAGGGAA CAGAGCTGTG GCTGGGTGTG 
GATGCCCTGG GCCTAAATAT CTATGAGAAA GACGACAAAC TCACTCCAAA AATTGGATTC 
CCCTGGAGCG AAATCCGAAA CATTTCCTTC AACGATAAGA AGTTTGTCAT CAAGCCCATA 
GATAAAAAGG CTCCAGATTT CGTGTTCTAC GCTCCACGTT TGCGCATTAA TAAGCGCATC 
CTTCAGCTGT GCATGGGGAA CCACGAGCTG TACATGCGCC GCAGGAAGCC GGATACCATC 
GAGGTGCAGC AGATGAAAGC TCAGGCTCGA GAGGAGAAAC AACAGAAACA GATGGAGAGA 
GCTCAGCTGG AGAACGAGAA GAAGAGACGA GAGGCCATCG AGAGAGAGAA GGAGCAAATG 
GAAAGAGAGA AAAAGGAGCT GATGATGCAA CTCTACCAGT TCGAGGAGAG GACCAAAAAA 
GTAGAGAATG AATTGCAGGA GCAGATGCAG AGGGCCATGC AGCTGCAGCA CGAACGGAGA 
CTGGCTGATG AAGAAGCCGC TAGACTGGAG GCCGAGCGAC AGGCAGCACT GCTCGCCAAG 
GAGGAGCTGG CCCGTCAAGC ACAAGATCAG CTCAAGAGTC AGGAGCAGCT GGCTGCTGAG 
CTGGCAGAAT ACACTGCAAA AATTTCACTC CTTGAAGAAG CCAAAAAACG CAAGGAGGAA 
GAAGCTCAGA CATGGCAGAA CAAAGCACAA TTAGCTCAAG ATGACCTGAT AAAGACGCGT 
CAGGAGCTTC ACAATGTGAT GACGGCGCCA CCTCTTCCTC CTCCACCACC TGCATACGAC 
CATGATGAAA ATGATCTCGA TGATGGAGAG GAGAGCAATG GCAGCTACAC CGCAGACCTG 
CAGACGGGAG GATTCAATGA CCACCGCCTG GAGGAAGAGC GAATCACTGA GGCCGAGAAG 
AACGAACGAG TTCAGAAACA ACTGCTGGCT CTGACCTCAG AATTGGCCCA AGCTCGAGAC 
GACACCAAGA AGACTCAGAA CGACCTGCTG CACACAGAGA ACGTGCGCGC TGGCCGAGAC 
AAGTACAAAA CTCTCCGGCA GATCCGCCAG GGTAACACCA AACAGAGAAT CGACGAGTTC 
GAGGCCTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160494.1
CDS	97..1848
Translation	MPKPVNVRVTTMDAELEFAVQPSTTGKQLFDQVVKTIGLREIWYFGLQYMDSKGYLTWLKLDKKVSSQDVKKENPLQFKFRAKFFPEDVADELIQDITQKLFFMQVKDGILSDEIYCPPETAVLLASYSVQAKFGDFSKELHRPGYLTSERLLPQRVLDQHKLSREQWEERIQVWHEEHRGMLREDAMLEYLKIAQDLEMYGVNYFDIKNKKGTELWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKQQKQMERAQLENEKKRREAIEREKEQMEREKKELMMQLYQFEERTKKVENELQEQMQRAMQLQHERRLADEEAARLEAERQAALLAKEELARQAQDQLKSQEQLAAELAEYTAKISLLEEAKKRKEEEAQTWQNKAQLAQDDLIKTRQELHNVMTAPPLPPPPPAYDHDENDLDDGEESNGSYTADLQTGGFNDHRLEEERITEAEKNERVQKQLLALTSELAQARDDTKKTQNDLLHTENVRAGRDKYKTLRQIRQGNTKQRIDEFEAL

Target ORF information:

RefSeq Version	XM_005160437.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ezrin b (ezrb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160437.4

ORF Insert Sequence:

ATGCCTAAAC CAGTAAATGT TCGCGTCACT ACAATGGACG CTGAGCTGGA GTTTGCCGTT 
CAGCCCAGCA CAACTGGCAA GCAGCTGTTT GATCAGGTGG TGAAGACCAT CGGGTTACGT 
GAAATATGGT ACTTTGGGCT TCAATATATG GACAGCAAAG GCTACCTTAC ATGGCTGAAA 
CTTGACAAAA AGGTGTCTTC TCAGGACGTG AAGAAAGAGA ATCCGCTGCA GTTTAAGTTT 
CGTGCTAAAT TCTTCCCAGA AGACGTAGCG GATGAGCTGA TCCAGGACAT CACTCAAAAA 
CTCTTCTTCA TGCAAGTGAA AGATGGCATT CTGAGTGACG AGATCTATTG TCCTCCAGAG 
ACTGCCGTGC TCTTGGCGTC CTATTCAGTG CAGGCCAAGT TTGGGGATTT CAGCAAGGAG 
TTGCACAGGC CTGGATACCT GACATCAGAA CGCCTGCTGC CCCAACGGGT TCTGGATCAG 
CACAAGTTGT CACGGGAACA GTGGGAGGAG AGGATTCAGG TGTGGCACGA GGAACATCGG 
GGGATGCTCA GAGAGGACGC AATGCTGGAG TATTTGAAAA TCGCTCAGGA TCTGGAAATG 
TACGGCGTGA ACTATTTTGA CATCAAGAAC AAGAAGGGAA CAGAGCTGTG GCTGGGTGTG 
GATGCCCTGG GCCTAAATAT CTATGAGAAA GACGACAAAC TCACTCCAAA AATTGGATTC 
CCCTGGAGCG AAATCCGAAA CATTTCCTTC AACGATAAGA AGTTTGTCAT CAAGCCCATA 
GATAAAAAGG CTCCAGATTT CGTGTTCTAC GCTCCACGTT TGCGCATTAA TAAGCGCATC 
CTTCAGCTGT GCATGGGGAA CCACGAGCTG TACATGCGCC GCAGGAAGCC GGATACCATC 
GAGGTGCAGC AGATGAAAGC TCAGGCTCGA GAGGAGAAAC AACAGAAACA GATGGAGAGA 
GCTCAGCTGG AGAACGAGAA GAAGAGACGA GAGGCCATCG AGAGAGAGAA GGAGCAAATG 
GAAAGAGAGA AAAAGGAGCT GATGATGCAA CTCTACCAGT TCGAGGAGAG GACCAAAAAA 
GTAGAGAATG AATTGCAGGA GCAGATGCAG AGGGCCATGC AGCTGCAGCA CGAACGGAGA 
CTGGCTGATG AAGAAGCCGC TAGACTGGAG GCCGAGCGAC AGGCAGCACT GCTCGCCAAG 
GAGGAGCTGG CCCGTCAAGC ACAAGATCAG CTCAAGAGTC AGGAGCAGCT GGCTGCTGAG 
CTGGCAGAAT ACACTGCAAA AATTTCACTC CTTGAAGAAG CCAAAAAACG CAAGGAGGAA 
GAAGCTCAGA CATGGCAGAA CAAAGCACAA TTAGCTCAAG ATGACCTGAT AAAGACGCGT 
CAGGAGCTTC ACAATGTGAT GACGGCGCCA CCTCTTCCTC CTCCACCACC TGCATACGAC 
CATGATGAAA ATGATCTCGA TGATGGAGAG GAGAGCAATG GCAGCTACAC CGCAGACCTG 
CAGACGGGAG GATTCAATGA CCACCGCCTG GAGGAAGAGC GAATCACTGA GGCCGAGAAG 
AACGAACGAG TTCAGAAACA ACTGCTGGCT CTGACCTCAG AATTGGCCCA AGCTCGAGAC 
GACACCAAGA AGACTCAGAA CGACCTGCTG CACACAGAGA ACGTGCGCGC TGGCCGAGAC 
AAGTACAAAA CTCTCCGGCA GATCCGCCAG GGTAACACCA AACAGAGAAT CGACGAGTTC 
GAGGCCTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ezrb ( XM_005160437.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160494.1 Danio rerio ezrin b (ezrb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa00066
Clone ID Related Accession (Same CDS sequence)	XM_005160437.4 , XM_005160437.3 , NM_001030285.2
Accession Version	XM_005160437.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	villin 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160437.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTAAAC CAGTAAATGT TCGCGTCACT ACAATGGACG CTGAGCTGGA GTTTGCCGTT 
CAGCCCAGCA CAACTGGCAA GCAGCTGTTT GATCAGGTGG TGAAGACCAT CGGGTTACGT 
GAAATATGGT ACTTTGGGCT TCAATATATG GACAGCAAAG GCTACCTTAC ATGGCTGAAA 
CTTGACAAAA AGGTGTCTTC TCAGGACGTG AAGAAAGAGA ATCCGCTGCA GTTTAAGTTT 
CGTGCTAAAT TCTTCCCAGA AGACGTAGCG GATGAGCTGA TCCAGGACAT CACTCAAAAA 
CTCTTCTTCA TGCAAGTGAA AGATGGCATT CTGAGTGACG AGATCTATTG TCCTCCAGAG 
ACTGCCGTGC TCTTGGCGTC CTATTCAGTG CAGGCCAAGT TTGGGGATTT CAGCAAGGAG 
TTGCACAGGC CTGGATACCT GACATCAGAA CGCCTGCTGC CCCAACGGGT TCTGGATCAG 
CACAAGTTGT CACGGGAACA GTGGGAGGAG AGGATTCAGG TGTGGCACGA GGAACATCGG 
GGGATGCTCA GAGAGGACGC AATGCTGGAG TATTTGAAAA TCGCTCAGGA TCTGGAAATG 
TACGGCGTGA ACTATTTTGA CATCAAGAAC AAGAAGGGAA CAGAGCTGTG GCTGGGTGTG 
GATGCCCTGG GCCTAAATAT CTATGAGAAA GACGACAAAC TCACTCCAAA AATTGGATTC 
CCCTGGAGCG AAATCCGAAA CATTTCCTTC AACGATAAGA AGTTTGTCAT CAAGCCCATA 
GATAAAAAGG CTCCAGATTT CGTGTTCTAC GCTCCACGTT TGCGCATTAA TAAGCGCATC 
CTTCAGCTGT GCATGGGGAA CCACGAGCTG TACATGCGCC GCAGGAAGCC GGATACCATC 
GAGGTGCAGC AGATGAAAGC TCAGGCTCGA GAGGAGAAAC AACAGAAACA GATGGAGAGA 
GCTCAGCTGG AGAACGAGAA GAAGAGACGA GAGGCCATCG AGAGAGAGAA GGAGCAAATG 
GAAAGAGAGA AAAAGGAGCT GATGATGCAA CTCTACCAGT TCGAGGAGAG GACCAAAAAA 
GTAGAGAATG AATTGCAGGA GCAGATGCAG AGGGCCATGC AGCTGCAGCA CGAACGGAGA 
CTGGCTGATG AAGAAGCCGC TAGACTGGAG GCCGAGCGAC AGGCAGCACT GCTCGCCAAG 
GAGGAGCTGG CCCGTCAAGC ACAAGATCAG CTCAAGAGTC AGGAGCAGCT GGCTGCTGAG 
CTGGCAGAAT ACACTGCAAA AATTTCACTC CTTGAAGAAG CCAAAAAACG CAAGGAGGAA 
GAAGCTCAGA CATGGCAGAA CAAAGCACAA TTAGCTCAAG ATGACCTGAT AAAGACGCGT 
CAGGAGCTTC ACAATGTGAT GACGGCGCCA CCTCTTCCTC CTCCACCACC TGCATACGAC 
CATGATGAAA ATGATCTCGA TGATGGAGAG GAGAGCAATG GCAGCTACAC CGCAGACCTG 
CAGACGGGAG GATTCAATGA CCACCGCCTG GAGGAAGAGC GAATCACTGA GGCCGAGAAG 
AACGAACGAG TTCAGAAACA ACTGCTGGCT CTGACCTCAG AATTGGCCCA AGCTCGAGAC 
GACACCAAGA AGACTCAGAA CGACCTGCTG CACACAGAGA ACGTGCGCGC TGGCCGAGAC 
AAGTACAAAA CTCTCCGGCA GATCCGCCAG GGTAACACCA AACAGAGAAT CGACGAGTTC 
GAGGCCTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160494.1
CDS	95..1846
Translation	MPKPVNVRVTTMDAELEFAVQPSTTGKQLFDQVVKTIGLREIWYFGLQYMDSKGYLTWLKLDKKVSSQDVKKENPLQFKFRAKFFPEDVADELIQDITQKLFFMQVKDGILSDEIYCPPETAVLLASYSVQAKFGDFSKELHRPGYLTSERLLPQRVLDQHKLSREQWEERIQVWHEEHRGMLREDAMLEYLKIAQDLEMYGVNYFDIKNKKGTELWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKQQKQMERAQLENEKKRREAIEREKEQMEREKKELMMQLYQFEERTKKVENELQEQMQRAMQLQHERRLADEEAARLEAERQAALLAKEELARQAQDQLKSQEQLAAELAEYTAKISLLEEAKKRKEEEAQTWQNKAQLAQDDLIKTRQELHNVMTAPPLPPPPPAYDHDENDLDDGEESNGSYTADLQTGGFNDHRLEEERITEAEKNERVQKQLLALTSELAQARDDTKKTQNDLLHTENVRAGRDKYKTLRQIRQGNTKQRIDEFEAL

Target ORF information:

RefSeq Version	XM_005160437.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ezrin b (ezrb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160437.3

ORF Insert Sequence:

ATGCCTAAAC CAGTAAATGT TCGCGTCACT ACAATGGACG CTGAGCTGGA GTTTGCCGTT 
CAGCCCAGCA CAACTGGCAA GCAGCTGTTT GATCAGGTGG TGAAGACCAT CGGGTTACGT 
GAAATATGGT ACTTTGGGCT TCAATATATG GACAGCAAAG GCTACCTTAC ATGGCTGAAA 
CTTGACAAAA AGGTGTCTTC TCAGGACGTG AAGAAAGAGA ATCCGCTGCA GTTTAAGTTT 
CGTGCTAAAT TCTTCCCAGA AGACGTAGCG GATGAGCTGA TCCAGGACAT CACTCAAAAA 
CTCTTCTTCA TGCAAGTGAA AGATGGCATT CTGAGTGACG AGATCTATTG TCCTCCAGAG 
ACTGCCGTGC TCTTGGCGTC CTATTCAGTG CAGGCCAAGT TTGGGGATTT CAGCAAGGAG 
TTGCACAGGC CTGGATACCT GACATCAGAA CGCCTGCTGC CCCAACGGGT TCTGGATCAG 
CACAAGTTGT CACGGGAACA GTGGGAGGAG AGGATTCAGG TGTGGCACGA GGAACATCGG 
GGGATGCTCA GAGAGGACGC AATGCTGGAG TATTTGAAAA TCGCTCAGGA TCTGGAAATG 
TACGGCGTGA ACTATTTTGA CATCAAGAAC AAGAAGGGAA CAGAGCTGTG GCTGGGTGTG 
GATGCCCTGG GCCTAAATAT CTATGAGAAA GACGACAAAC TCACTCCAAA AATTGGATTC 
CCCTGGAGCG AAATCCGAAA CATTTCCTTC AACGATAAGA AGTTTGTCAT CAAGCCCATA 
GATAAAAAGG CTCCAGATTT CGTGTTCTAC GCTCCACGTT TGCGCATTAA TAAGCGCATC 
CTTCAGCTGT GCATGGGGAA CCACGAGCTG TACATGCGCC GCAGGAAGCC GGATACCATC 
GAGGTGCAGC AGATGAAAGC TCAGGCTCGA GAGGAGAAAC AACAGAAACA GATGGAGAGA 
GCTCAGCTGG AGAACGAGAA GAAGAGACGA GAGGCCATCG AGAGAGAGAA GGAGCAAATG 
GAAAGAGAGA AAAAGGAGCT GATGATGCAA CTCTACCAGT TCGAGGAGAG GACCAAAAAA 
GTAGAGAATG AATTGCAGGA GCAGATGCAG AGGGCCATGC AGCTGCAGCA CGAACGGAGA 
CTGGCTGATG AAGAAGCCGC TAGACTGGAG GCCGAGCGAC AGGCAGCACT GCTCGCCAAG 
GAGGAGCTGG CCCGTCAAGC ACAAGATCAG CTCAAGAGTC AGGAGCAGCT GGCTGCTGAG 
CTGGCAGAAT ACACTGCAAA AATTTCACTC CTTGAAGAAG CCAAAAAACG CAAGGAGGAA 
GAAGCTCAGA CATGGCAGAA CAAAGCACAA TTAGCTCAAG ATGACCTGAT AAAGACGCGT 
CAGGAGCTTC ACAATGTGAT GACGGCGCCA CCTCTTCCTC CTCCACCACC TGCATACGAC 
CATGATGAAA ATGATCTCGA TGATGGAGAG GAGAGCAATG GCAGCTACAC CGCAGACCTG 
CAGACGGGAG GATTCAATGA CCACCGCCTG GAGGAAGAGC GAATCACTGA GGCCGAGAAG 
AACGAACGAG TTCAGAAACA ACTGCTGGCT CTGACCTCAG AATTGGCCCA AGCTCGAGAC 
GACACCAAGA AGACTCAGAA CGACCTGCTG CACACAGAGA ACGTGCGCGC TGGCCGAGAC 
AAGTACAAAA CTCTCCGGCA GATCCGCCAG GGTAACACCA AACAGAGAAT CGACGAGTTC 
GAGGCCTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

The Joubert Syndrome Protein Inpp5e Controls Ciliogenesis by Regulating Phosphoinositides at the Apical Membrane.
Journal of the American Society of Nephrology : JASN28(1)118-129(2017 Jan)
Xu W,Jin M,Hu R,Wang H,Zhang F,Yuan S,Cao Y

Steering cell migration by alternating blebs and actin-rich protrusions.
BMC biology1474(2016 Sep)
Diz-Mu?oz A,Romanczuk P,Yu W,Bergert M,Ivanovitch K,Salbreux G,Heisenberg CP,Paluch EK

Identification of Novel Ezrin Inhibitors Targeting Metastatic Osteosarcoma by Screening Open Access Malaria Box.
Molecular cancer therapeutics14(11)2497-507(2015 Nov)
?elik H,Hong SH,Col?n-L?pez DD,Han J,Kont YS,Minas TZ,Swift M,Paige M,Glasgow E,Toretsky JA,Bosch J,?ren A

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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ezrb cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム