top3a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	top3a
Entrez Gene ID	560212
Full Name	DNA topoisomerase III alpha
General protein information	Preferred Names DNA topoisomerase III alpha Names DNA topoisomerase 3-alpha topoisomerase (DNA) III alpha
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_683603.8	XP_688695.4	DNA topoisomerase 3-alpha
XM_683603.9	XP_688695.4	DNA topoisomerase 3-alpha

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	top3a	XP_688695.4
Pan troglodytes (chimpanzee)	TOP3A	XP_511323.2
	TOP3A	XP_001095437.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010028	XP_319175.3
Caenorhabditis elegans (roundworm)	top-3	NP_499558.1
Xenopus tropicalis (tropical clawed frog)	top3a	XP_002935889.1
Homo sapiens (human)	TOP3A	NP_004609.1
Bos taurus (cattle)	TOP3A	NP_001071443.1
Mus musculus (house mouse)	Top3a	NP_033436.1
Rattus norvegicus (Norway rat)	Top3a	XP_006246613.1
Gallus gallus (chicken)	TOP3A	NP_001025807.1
Arabidopsis thaliana (thale cress)	TOP3A	NP_201197.1
Canis lupus familiaris (dog)	TOP3A	XP_546656.2
Drosophila melanogaster (fruit fly)	Top3alpha	NP_523602.2
Oryza sativa (rice)	Os03g0165000	NP_001173278.1

Gene Ontology

The following top3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the top3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa08651	XM_683603.9 Latest version!	Danio rerio topoisomerase (DNA) III alpha (top3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa08651	XM_683603.8	Danio rerio topoisomerase (DNA) III alpha (top3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

top3a ( XM_683603.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_688695.4 Danio rerio topoisomerase (DNA) III alpha (top3a), mRNA.

Price & Availability↑

CloneID	ODa08651
Clone ID Related Accession (Same CDS sequence)	XM_683603.8 , XM_683603.9
Accession Version	XM_683603.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DNA topoisomerase 3-alpha
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_683603.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGTTT TGTTTAGGGC ACTAAGAAAA ACTGTCAACA GTCGATTTTA TAGACATATA 
TCTGTAAGCA TGATAAGACG CACTCAGATT AAGAAAGTGC TGTGTGTGGC CGAGAAAAAC 
GATGCAGCTA AAGGCATAGC AGAGATCATG TCAAATGGCA GATCCAGAAG AAGAGAAGGC 
TGCTCTGTTT ACAACAAAAT ATATGAGTAT GAATACAACC TGTTCGGCCA AAATGTTACT 
GTAAACATGA CGTCAGTATC AGGGCATCTC TTGGCTCTTG AATTCAAAGC TCCTTTTCAG 
AAATGGCATA GTTGTAGTCC AGTCCTATTG TTTGATGCTG AGGTGGAAAA GTACTGCCCT 
GAGAACTTTA TACCGATTAA GAGGACTTTG GAGAGAGAGG TGAGGCAGTG TCAAGCTCTG 
ATTGTTTGGA CTGATTGTGA CAGGGAAGGA GAGAATATCG GCTTCGAGAT CATTGATGTC 
TGCAAAGCAG TGAAGCCAAA CATCCAGGTC TTCCGTGCAC GGTTCTCTGA GATCACCCCA 
AACTCCATTA GGAGAGCCTG TGAGACCCTC ACGGAGCCTG ATATCAATGT TAGCGATGCG 
GTCGACGTGA GACAGGAGCT AGATCTTCGC ATTGGGGCAT CGTTTACCAG ATTCCAGACT 
TTGCGCCTGC AGAAGATATT CCCAGAATCT CTCTCTGATC AGCTCATCAG TTATGGCAGC 
TGTCAGTTCC CCACTCTTGG CTTTGTGGTA GAACGATTTA AAGCCATTCA AGCCTTCATC 
CCTGAAACAT TCTTCAAAAT CAAAGTTGTC CATGAACCTA ATGAAGAGGA GTCAGTGGAA 
TTCAACTGGA AAAGACATCG ACTGTTCAAT CACACCGCAT GCCTTGTGCT GTATCAGATG 
TGCATGGAGG AACCCATGGC GAAAGTTATA TCTGTAACAA GTAAACCAAA AAGCAAATGG 
CGACCTTTAC CACTGGACAC TGTGGAACTT GAAAAACTGG CCTCCAGAAA ATTGAGGATA 
AGTGCCAAAG AAACCATGAA GATTGCAGAA AAGCTGTATA CTCAAGGTTT TATCAGTTAT 
CCTCGCACGG AGACCAACAT GTTTCCACAG AACCTGAACC TTACCCGTCT GGTTGAGCAA 
CAGACACAGG ACCAAGAATG GGGAAATTTT GCCCAGCGTA TTTTGGAAAG CGGTGGGCCC 
ACTCCTCGAA ACGGAAACAA ATCAGATCAG GCCCATCCCC CCATTCACCC CACCAAATAC 
ACCAACGGTT TACAGGGAAA TGAAAAGAGA CTTTATGAGT TCATTGTACG TCACTTCCTG 
GCCTGTTGCT CCAAAGATGC TCAGGGTCAA GAGGTCACAG TCGAAATTGA TATAGCGGAA 
GAGAGATTCT CTGCTAGCGG ACTGACGATC ATCGCTCGCA ATTATCTGGA AGTGTACCCT 
TATGACAAGT GGTACAATAA GGTGATTCCA TCATACAGCC CTAACACCAC ATTCCAGCCC 
ACAGCGATTG AGATGGTGGA AGGACAGACC AGTCCACCAC AGCTTCTGAC AGAGGCAGAT 
TTGATATCAC TCATGGAGAA ACATGGCATT GGTACCGATG CAACACATGC TGAACACATT 
GAGACCATTA AGAGTCGCAT GTATGTGGGG CTGACGGCAG ACCAGAGGTT CCTTCCCGGA 
GAGCTCGGCA TGGGTCTCGT TGAAGGATAT AATTCTATGG GATACGAGAT GTCTAAACCA 
GACCTGAGAG CTGAGCTGGA GGCAGACCTT AAACTTGTGT CAGAGGGCCG GAAGAATAAA 
GCATCTGTCC TCAGTTATCA TGTGGCAAAA TACAAAGCCG TTTTCATCGA GTCTGTGAGG 
AAGGCAAAGA AATTAGATGA GGCTTTGTCC AATTATCTGG GTCCGGCTCA GGAGATTGCA 
GAACAGGAGC AAGTCGAGTT GGAGATACCA TTACCTGTGC GAAAGTGTCC TCAGTGTAAC 
CGAGACATGG TGCTGAAGAA GAAGAAAGAC AGCACAGGGA TGCTTCTTTC ATGCTTGGGA 
TATCCAGCGT GTAAAGCAGC CGTGTGGTTT CCTGACACTG TCCTGGAAGT CAGCAGAGAC 
GAGAGCATCT GTCCAACTTG CCGTCCACAT CCGGTTCATA TGCTGAAGTT TAAATTCAAG 
AGGGGAAGTT TGCCACCCAT GATGCCTCTT GAATTTGTTG GATGCATCGG AGGATGTGAT 
GACACTCTCA GAGAGGTTTT AAACTTGAAA TATCTCAGAG GACGTGATGC AGAATCTGCC 
CCTCAGGCCA ATAACAGCTC CAGATCCAGT CAACACCACA TTCATAGAAC CAGCTCGGCA 
CCTCCTCCAT CCAGGGTAGA GAACATTAGA CCTCCAGCAC CCAGACCTCG AGCTGATCCT 
GGTCTGCAAC CTCCACCTTC AACTCAGGGC GGTGCCATTG TGTGTAACTG TGGTCAGGAC 
GCCTTACTTC TCACCGTGCG CAAAGAGGGT CCCAATCAAG GGCGTCAGTT TTACAAGTGT 
AACACAGGAG ACTGCAAGTT CTTCCTGTGG GCCGATCAGC CACCTGAACA GGGGGTGCCT 
GAAAGAAACA GAGGGCCTTT AAGTCAAAAC GTCCAGCCTC CCAGGCCTTC CAATGGGTTT 
GGGAATGTCT CACAGCAGAG GCAGAACCAC AGAGGGTCTG GAGGCGATGA AGGGGAAACT 
ATGTGTAACT GTAATGAGCC TTCAGTCACC AGGACTGTCA TGAAGGAAGG GCCGAACAAG 
GGCAGAATGT TTCACACCTG CGGGAAACCA CGAGATCAGC AGTGTGGCTT CTTCCAGTGG 
GCCAATGAAA ATGTGGCACC AGCCACAGCG TTTTCTGGAG GAAATGGTGG TAAACCATCT 
GGGAACAAAA AGGCCAGTAC TACCTCAGTC AAGCCTCCCA CTGCCAAAAG GCAGAGGACC 
TGTGGAATAT GTCATGAGCC AGGACACACC AGAGTCACCT GTCCTCAGGC CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_688695.4
CDS	650..3646
Translation	MNVLFRALRKTVNSRFYRHISVSMIRRTQIKKVLCVAEKNDAAKGIAEIMSNGRSRRREGCSVYNKIYEYEYNLFGQNVTVNMTSVSGHLLALEFKAPFQKWHSCSPVLLFDAEVEKYCPENFIPIKRTLEREVRQCQALIVWTDCDREGENIGFEIIDVCKAVKPNIQVFRARFSEITPNSIRRACETLTEPDINVSDAVDVRQELDLRIGASFTRFQTLRLQKIFPESLSDQLISYGSCQFPTLGFVVERFKAIQAFIPETFFKIKVVHEPNEEESVEFNWKRHRLFNHTACLVLYQMCMEEPMAKVISVTSKPKSKWRPLPLDTVELEKLASRKLRISAKETMKIAEKLYTQGFISYPRTETNMFPQNLNLTRLVEQQTQDQEWGNFAQRILESGGPTPRNGNKSDQAHPPIHPTKYTNGLQGNEKRLYEFIVRHFLACCSKDAQGQEVTVEIDIAEERFSASGLTIIARNYLEVYPYDKWYNKVIPSYSPNTTFQPTAIEMVEGQTSPPQLLTEADLISLMEKHGIGTDATHAEHIETIKSRMYVGLTADQRFLPGELGMGLVEGYNSMGYEMSKPDLRAELEADLKLVSEGRKNKASVLSYHVAKYKAVFIESVRKAKKLDEALSNYLGPAQEIAEQEQVELEIPLPVRKCPQCNRDMVLKKKKDSTGMLLSCLGYPACKAAVWFPDTVLEVSRDESICPTCRPHPVHMLKFKFKRGSLPPMMPLEFVGCIGGCDDTLREVLNLKYLRGRDAESAPQANNSSRSSQHHIHRTSSAPPPSRVENIRPPAPRPRADPGLQPPPSTQGGAIVCNCGQDALLLTVRKEGPNQGRQFYKCNTGDCKFFLWADQPPEQGVPERNRGPLSQNVQPPRPSNGFGNVSQQRQNHRGSGGDEGETMCNCNEPSVTRTVMKEGPNKGRMFHTCGKPRDQQCGFFQWANENVAPATAFSGGNGGKPSGNKKASTTSVKPPTAKRQRTCGICHEPGHTRVTCPQAH

Target ORF information:

RefSeq Version	XM_683603.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio topoisomerase (DNA) III alpha (top3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_683603.8

ORF Insert Sequence:

ATGAACGTTT TGTTTAGGGC ACTAAGAAAA ACTGTCAACA GTCGATTTTA TAGACATATA 
TCTGTAAGCA TGATAAGACG CACTCAGATT AAGAAAGTGC TGTGTGTGGC CGAGAAAAAC 
GATGCAGCTA AAGGCATAGC AGAGATCATG TCAAATGGCA GATCCAGAAG AAGAGAAGGC 
TGCTCTGTTT ACAACAAAAT ATATGAGTAT GAATACAACC TGTTCGGCCA AAATGTTACT 
GTAAACATGA CGTCAGTATC AGGGCATCTC TTGGCTCTTG AATTCAAAGC TCCTTTTCAG 
AAATGGCATA GTTGTAGTCC AGTCCTATTG TTTGATGCTG AGGTGGAAAA GTACTGCCCT 
GAGAACTTTA TACCGATTAA GAGGACTTTG GAGAGAGAGG TGAGGCAGTG TCAAGCTCTG 
ATTGTTTGGA CTGATTGTGA CAGGGAAGGA GAGAATATCG GCTTCGAGAT CATTGATGTC 
TGCAAAGCAG TGAAGCCAAA CATCCAGGTC TTCCGTGCAC GGTTCTCTGA GATCACCCCA 
AACTCCATTA GGAGAGCCTG TGAGACCCTC ACGGAGCCTG ATATCAATGT TAGCGATGCG 
GTCGACGTGA GACAGGAGCT AGATCTTCGC ATTGGGGCAT CGTTTACCAG ATTCCAGACT 
TTGCGCCTGC AGAAGATATT CCCAGAATCT CTCTCTGATC AGCTCATCAG TTATGGCAGC 
TGTCAGTTCC CCACTCTTGG CTTTGTGGTA GAACGATTTA AAGCCATTCA AGCCTTCATC 
CCTGAAACAT TCTTCAAAAT CAAAGTTGTC CATGAACCTA ATGAAGAGGA GTCAGTGGAA 
TTCAACTGGA AAAGACATCG ACTGTTCAAT CACACCGCAT GCCTTGTGCT GTATCAGATG 
TGCATGGAGG AACCCATGGC GAAAGTTATA TCTGTAACAA GTAAACCAAA AAGCAAATGG 
CGACCTTTAC CACTGGACAC TGTGGAACTT GAAAAACTGG CCTCCAGAAA ATTGAGGATA 
AGTGCCAAAG AAACCATGAA GATTGCAGAA AAGCTGTATA CTCAAGGTTT TATCAGTTAT 
CCTCGCACGG AGACCAACAT GTTTCCACAG AACCTGAACC TTACCCGTCT GGTTGAGCAA 
CAGACACAGG ACCAAGAATG GGGAAATTTT GCCCAGCGTA TTTTGGAAAG CGGTGGGCCC 
ACTCCTCGAA ACGGAAACAA ATCAGATCAG GCCCATCCCC CCATTCACCC CACCAAATAC 
ACCAACGGTT TACAGGGAAA TGAAAAGAGA CTTTATGAGT TCATTGTACG TCACTTCCTG 
GCCTGTTGCT CCAAAGATGC TCAGGGTCAA GAGGTCACAG TCGAAATTGA TATAGCGGAA 
GAGAGATTCT CTGCTAGCGG ACTGACGATC ATCGCTCGCA ATTATCTGGA AGTGTACCCT 
TATGACAAGT GGTACAATAA GGTGATTCCA TCATACAGCC CTAACACCAC ATTCCAGCCC 
ACAGCGATTG AGATGGTGGA AGGACAGACC AGTCCACCAC AGCTTCTGAC AGAGGCAGAT 
TTGATATCAC TCATGGAGAA ACATGGCATT GGTACCGATG CAACACATGC TGAACACATT 
GAGACCATTA AGAGTCGCAT GTATGTGGGG CTGACGGCAG ACCAGAGGTT CCTTCCCGGA 
GAGCTCGGCA TGGGTCTCGT TGAAGGATAT AATTCTATGG GATACGAGAT GTCTAAACCA 
GACCTGAGAG CTGAGCTGGA GGCAGACCTT AAACTTGTGT CAGAGGGCCG GAAGAATAAA 
GCATCTGTCC TCAGTTATCA TGTGGCAAAA TACAAAGCCG TTTTCATCGA GTCTGTGAGG 
AAGGCAAAGA AATTAGATGA GGCTTTGTCC AATTATCTGG GTCCGGCTCA GGAGATTGCA 
GAACAGGAGC AAGTCGAGTT GGAGATACCA TTACCTGTGC GAAAGTGTCC TCAGTGTAAC 
CGAGACATGG TGCTGAAGAA GAAGAAAGAC AGCACAGGGA TGCTTCTTTC ATGCTTGGGA 
TATCCAGCGT GTAAAGCAGC CGTGTGGTTT CCTGACACTG TCCTGGAAGT CAGCAGAGAC 
GAGAGCATCT GTCCAACTTG CCGTCCACAT CCGGTTCATA TGCTGAAGTT TAAATTCAAG 
AGGGGAAGTT TGCCACCCAT GATGCCTCTT GAATTTGTTG GATGCATCGG AGGATGTGAT 
GACACTCTCA GAGAGGTTTT AAACTTGAAA TATCTCAGAG GACGTGATGC AGAATCTGCC 
CCTCAGGCCA ATAACAGCTC CAGATCCAGT CAACACCACA TTCATAGAAC CAGCTCGGCA 
CCTCCTCCAT CCAGGGTAGA GAACATTAGA CCTCCAGCAC CCAGACCTCG AGCTGATCCT 
GGTCTGCAAC CTCCACCTTC AACTCAGGGC GGTGCCATTG TGTGTAACTG TGGTCAGGAC 
GCCTTACTTC TCACCGTGCG CAAAGAGGGT CCCAATCAAG GGCGTCAGTT TTACAAGTGT 
AACACAGGAG ACTGCAAGTT CTTCCTGTGG GCCGATCAGC CACCTGAACA GGGGGTGCCT 
GAAAGAAACA GAGGGCCTTT AAGTCAAAAC GTCCAGCCTC CCAGGCCTTC CAATGGGTTT 
GGGAATGTCT CACAGCAGAG GCAGAACCAC AGAGGGTCTG GAGGCGATGA AGGGGAAACT 
ATGTGTAACT GTAATGAGCC TTCAGTCACC AGGACTGTCA TGAAGGAAGG GCCGAACAAG 
GGCAGAATGT TTCACACCTG CGGGAAACCA CGAGATCAGC AGTGTGGCTT CTTCCAGTGG 
GCCAATGAAA ATGTGGCACC AGCCACAGCG TTTTCTGGAG GAAATGGTGG TAAACCATCT 
GGGAACAAAA AGGCCAGTAC TACCTCAGTC AAGCCTCCCA CTGCCAAAAG GCAGAGGACC 
TGTGGAATAT GTCATGAGCC AGGACACACC AGAGTCACCT GTCCTCAGGC CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

top3a ( XM_683603.9 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_688695.4 Danio rerio topoisomerase (DNA) III alpha (top3a), mRNA.

Price & Availability↑

CloneID	ODa08651
Clone ID Related Accession (Same CDS sequence)	XM_683603.8 , XM_683603.9
Accession Version	XM_683603.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DNA topoisomerase 3-alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_683603.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGTTT TGTTTAGGGC ACTAAGAAAA ACTGTCAACA GTCGATTTTA TAGACATATA 
TCTGTAAGCA TGATAAGACG CACTCAGATT AAGAAAGTGC TGTGTGTGGC CGAGAAAAAC 
GATGCAGCTA AAGGCATAGC AGAGATCATG TCAAATGGCA GATCCAGAAG AAGAGAAGGC 
TGCTCTGTTT ACAACAAAAT ATATGAGTAT GAATACAACC TGTTCGGCCA AAATGTTACT 
GTAAACATGA CGTCAGTATC AGGGCATCTC TTGGCTCTTG AATTCAAAGC TCCTTTTCAG 
AAATGGCATA GTTGTAGTCC AGTCCTATTG TTTGATGCTG AGGTGGAAAA GTACTGCCCT 
GAGAACTTTA TACCGATTAA GAGGACTTTG GAGAGAGAGG TGAGGCAGTG TCAAGCTCTG 
ATTGTTTGGA CTGATTGTGA CAGGGAAGGA GAGAATATCG GCTTCGAGAT CATTGATGTC 
TGCAAAGCAG TGAAGCCAAA CATCCAGGTC TTCCGTGCAC GGTTCTCTGA GATCACCCCA 
AACTCCATTA GGAGAGCCTG TGAGACCCTC ACGGAGCCTG ATATCAATGT TAGCGATGCG 
GTCGACGTGA GACAGGAGCT AGATCTTCGC ATTGGGGCAT CGTTTACCAG ATTCCAGACT 
TTGCGCCTGC AGAAGATATT CCCAGAATCT CTCTCTGATC AGCTCATCAG TTATGGCAGC 
TGTCAGTTCC CCACTCTTGG CTTTGTGGTA GAACGATTTA AAGCCATTCA AGCCTTCATC 
CCTGAAACAT TCTTCAAAAT CAAAGTTGTC CATGAACCTA ATGAAGAGGA GTCAGTGGAA 
TTCAACTGGA AAAGACATCG ACTGTTCAAT CACACCGCAT GCCTTGTGCT GTATCAGATG 
TGCATGGAGG AACCCATGGC GAAAGTTATA TCTGTAACAA GTAAACCAAA AAGCAAATGG 
CGACCTTTAC CACTGGACAC TGTGGAACTT GAAAAACTGG CCTCCAGAAA ATTGAGGATA 
AGTGCCAAAG AAACCATGAA GATTGCAGAA AAGCTGTATA CTCAAGGTTT TATCAGTTAT 
CCTCGCACGG AGACCAACAT GTTTCCACAG AACCTGAACC TTACCCGTCT GGTTGAGCAA 
CAGACACAGG ACCAAGAATG GGGAAATTTT GCCCAGCGTA TTTTGGAAAG CGGTGGGCCC 
ACTCCTCGAA ACGGAAACAA ATCAGATCAG GCCCATCCCC CCATTCACCC CACCAAATAC 
ACCAACGGTT TACAGGGAAA TGAAAAGAGA CTTTATGAGT TCATTGTACG TCACTTCCTG 
GCCTGTTGCT CCAAAGATGC TCAGGGTCAA GAGGTCACAG TCGAAATTGA TATAGCGGAA 
GAGAGATTCT CTGCTAGCGG ACTGACGATC ATCGCTCGCA ATTATCTGGA AGTGTACCCT 
TATGACAAGT GGTACAATAA GGTGATTCCA TCATACAGCC CTAACACCAC ATTCCAGCCC 
ACAGCGATTG AGATGGTGGA AGGACAGACC AGTCCACCAC AGCTTCTGAC AGAGGCAGAT 
TTGATATCAC TCATGGAGAA ACATGGCATT GGTACCGATG CAACACATGC TGAACACATT 
GAGACCATTA AGAGTCGCAT GTATGTGGGG CTGACGGCAG ACCAGAGGTT CCTTCCCGGA 
GAGCTCGGCA TGGGTCTCGT TGAAGGATAT AATTCTATGG GATACGAGAT GTCTAAACCA 
GACCTGAGAG CTGAGCTGGA GGCAGACCTT AAACTTGTGT CAGAGGGCCG GAAGAATAAA 
GCATCTGTCC TCAGTTATCA TGTGGCAAAA TACAAAGCCG TTTTCATCGA GTCTGTGAGG 
AAGGCAAAGA AATTAGATGA GGCTTTGTCC AATTATCTGG GTCCGGCTCA GGAGATTGCA 
GAACAGGAGC AAGTCGAGTT GGAGATACCA TTACCTGTGC GAAAGTGTCC TCAGTGTAAC 
CGAGACATGG TGCTGAAGAA GAAGAAAGAC AGCACAGGGA TGCTTCTTTC ATGCTTGGGA 
TATCCAGCGT GTAAAGCAGC CGTGTGGTTT CCTGACACTG TCCTGGAAGT CAGCAGAGAC 
GAGAGCATCT GTCCAACTTG CCGTCCACAT CCGGTTCATA TGCTGAAGTT TAAATTCAAG 
AGGGGAAGTT TGCCACCCAT GATGCCTCTT GAATTTGTTG GATGCATCGG AGGATGTGAT 
GACACTCTCA GAGAGGTTTT AAACTTGAAA TATCTCAGAG GACGTGATGC AGAATCTGCC 
CCTCAGGCCA ATAACAGCTC CAGATCCAGT CAACACCACA TTCATAGAAC CAGCTCGGCA 
CCTCCTCCAT CCAGGGTAGA GAACATTAGA CCTCCAGCAC CCAGACCTCG AGCTGATCCT 
GGTCTGCAAC CTCCACCTTC AACTCAGGGC GGTGCCATTG TGTGTAACTG TGGTCAGGAC 
GCCTTACTTC TCACCGTGCG CAAAGAGGGT CCCAATCAAG GGCGTCAGTT TTACAAGTGT 
AACACAGGAG ACTGCAAGTT CTTCCTGTGG GCCGATCAGC CACCTGAACA GGGGGTGCCT 
GAAAGAAACA GAGGGCCTTT AAGTCAAAAC GTCCAGCCTC CCAGGCCTTC CAATGGGTTT 
GGGAATGTCT CACAGCAGAG GCAGAACCAC AGAGGGTCTG GAGGCGATGA AGGGGAAACT 
ATGTGTAACT GTAATGAGCC TTCAGTCACC AGGACTGTCA TGAAGGAAGG GCCGAACAAG 
GGCAGAATGT TTCACACCTG CGGGAAACCA CGAGATCAGC AGTGTGGCTT CTTCCAGTGG 
GCCAATGAAA ATGTGGCACC AGCCACAGCG TTTTCTGGAG GAAATGGTGG TAAACCATCT 
GGGAACAAAA AGGCCAGTAC TACCTCAGTC AAGCCTCCCA CTGCCAAAAG GCAGAGGACC 
TGTGGAATAT GTCATGAGCC AGGACACACC AGAGTCACCT GTCCTCAGGC CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_688695.4
CDS	24..3020
Translation	MNVLFRALRKTVNSRFYRHISVSMIRRTQIKKVLCVAEKNDAAKGIAEIMSNGRSRRREGCSVYNKIYEYEYNLFGQNVTVNMTSVSGHLLALEFKAPFQKWHSCSPVLLFDAEVEKYCPENFIPIKRTLEREVRQCQALIVWTDCDREGENIGFEIIDVCKAVKPNIQVFRARFSEITPNSIRRACETLTEPDINVSDAVDVRQELDLRIGASFTRFQTLRLQKIFPESLSDQLISYGSCQFPTLGFVVERFKAIQAFIPETFFKIKVVHEPNEEESVEFNWKRHRLFNHTACLVLYQMCMEEPMAKVISVTSKPKSKWRPLPLDTVELEKLASRKLRISAKETMKIAEKLYTQGFISYPRTETNMFPQNLNLTRLVEQQTQDQEWGNFAQRILESGGPTPRNGNKSDQAHPPIHPTKYTNGLQGNEKRLYEFIVRHFLACCSKDAQGQEVTVEIDIAEERFSASGLTIIARNYLEVYPYDKWYNKVIPSYSPNTTFQPTAIEMVEGQTSPPQLLTEADLISLMEKHGIGTDATHAEHIETIKSRMYVGLTADQRFLPGELGMGLVEGYNSMGYEMSKPDLRAELEADLKLVSEGRKNKASVLSYHVAKYKAVFIESVRKAKKLDEALSNYLGPAQEIAEQEQVELEIPLPVRKCPQCNRDMVLKKKKDSTGMLLSCLGYPACKAAVWFPDTVLEVSRDESICPTCRPHPVHMLKFKFKRGSLPPMMPLEFVGCIGGCDDTLREVLNLKYLRGRDAESAPQANNSSRSSQHHIHRTSSAPPPSRVENIRPPAPRPRADPGLQPPPSTQGGAIVCNCGQDALLLTVRKEGPNQGRQFYKCNTGDCKFFLWADQPPEQGVPERNRGPLSQNVQPPRPSNGFGNVSQQRQNHRGSGGDEGETMCNCNEPSVTRTVMKEGPNKGRMFHTCGKPRDQQCGFFQWANENVAPATAFSGGNGGKPSGNKKASTTSVKPPTAKRQRTCGICHEPGHTRVTCPQAH

Target ORF information:

RefSeq Version	XM_683603.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio topoisomerase (DNA) III alpha (top3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_683603.9

ORF Insert Sequence:

ATGAACGTTT TGTTTAGGGC ACTAAGAAAA ACTGTCAACA GTCGATTTTA TAGACATATA 
TCTGTAAGCA TGATAAGACG CACTCAGATT AAGAAAGTGC TGTGTGTGGC CGAGAAAAAC 
GATGCAGCTA AAGGCATAGC AGAGATCATG TCAAATGGCA GATCCAGAAG AAGAGAAGGC 
TGCTCTGTTT ACAACAAAAT ATATGAGTAT GAATACAACC TGTTCGGCCA AAATGTTACT 
GTAAACATGA CGTCAGTATC AGGGCATCTC TTGGCTCTTG AATTCAAAGC TCCTTTTCAG 
AAATGGCATA GTTGTAGTCC AGTCCTATTG TTTGATGCTG AGGTGGAAAA GTACTGCCCT 
GAGAACTTTA TACCGATTAA GAGGACTTTG GAGAGAGAGG TGAGGCAGTG TCAAGCTCTG 
ATTGTTTGGA CTGATTGTGA CAGGGAAGGA GAGAATATCG GCTTCGAGAT CATTGATGTC 
TGCAAAGCAG TGAAGCCAAA CATCCAGGTC TTCCGTGCAC GGTTCTCTGA GATCACCCCA 
AACTCCATTA GGAGAGCCTG TGAGACCCTC ACGGAGCCTG ATATCAATGT TAGCGATGCG 
GTCGACGTGA GACAGGAGCT AGATCTTCGC ATTGGGGCAT CGTTTACCAG ATTCCAGACT 
TTGCGCCTGC AGAAGATATT CCCAGAATCT CTCTCTGATC AGCTCATCAG TTATGGCAGC 
TGTCAGTTCC CCACTCTTGG CTTTGTGGTA GAACGATTTA AAGCCATTCA AGCCTTCATC 
CCTGAAACAT TCTTCAAAAT CAAAGTTGTC CATGAACCTA ATGAAGAGGA GTCAGTGGAA 
TTCAACTGGA AAAGACATCG ACTGTTCAAT CACACCGCAT GCCTTGTGCT GTATCAGATG 
TGCATGGAGG AACCCATGGC GAAAGTTATA TCTGTAACAA GTAAACCAAA AAGCAAATGG 
CGACCTTTAC CACTGGACAC TGTGGAACTT GAAAAACTGG CCTCCAGAAA ATTGAGGATA 
AGTGCCAAAG AAACCATGAA GATTGCAGAA AAGCTGTATA CTCAAGGTTT TATCAGTTAT 
CCTCGCACGG AGACCAACAT GTTTCCACAG AACCTGAACC TTACCCGTCT GGTTGAGCAA 
CAGACACAGG ACCAAGAATG GGGAAATTTT GCCCAGCGTA TTTTGGAAAG CGGTGGGCCC 
ACTCCTCGAA ACGGAAACAA ATCAGATCAG GCCCATCCCC CCATTCACCC CACCAAATAC 
ACCAACGGTT TACAGGGAAA TGAAAAGAGA CTTTATGAGT TCATTGTACG TCACTTCCTG 
GCCTGTTGCT CCAAAGATGC TCAGGGTCAA GAGGTCACAG TCGAAATTGA TATAGCGGAA 
GAGAGATTCT CTGCTAGCGG ACTGACGATC ATCGCTCGCA ATTATCTGGA AGTGTACCCT 
TATGACAAGT GGTACAATAA GGTGATTCCA TCATACAGCC CTAACACCAC ATTCCAGCCC 
ACAGCGATTG AGATGGTGGA AGGACAGACC AGTCCACCAC AGCTTCTGAC AGAGGCAGAT 
TTGATATCAC TCATGGAGAA ACATGGCATT GGTACCGATG CAACACATGC TGAACACATT 
GAGACCATTA AGAGTCGCAT GTATGTGGGG CTGACGGCAG ACCAGAGGTT CCTTCCCGGA 
GAGCTCGGCA TGGGTCTCGT TGAAGGATAT AATTCTATGG GATACGAGAT GTCTAAACCA 
GACCTGAGAG CTGAGCTGGA GGCAGACCTT AAACTTGTGT CAGAGGGCCG GAAGAATAAA 
GCATCTGTCC TCAGTTATCA TGTGGCAAAA TACAAAGCCG TTTTCATCGA GTCTGTGAGG 
AAGGCAAAGA AATTAGATGA GGCTTTGTCC AATTATCTGG GTCCGGCTCA GGAGATTGCA 
GAACAGGAGC AAGTCGAGTT GGAGATACCA TTACCTGTGC GAAAGTGTCC TCAGTGTAAC 
CGAGACATGG TGCTGAAGAA GAAGAAAGAC AGCACAGGGA TGCTTCTTTC ATGCTTGGGA 
TATCCAGCGT GTAAAGCAGC CGTGTGGTTT CCTGACACTG TCCTGGAAGT CAGCAGAGAC 
GAGAGCATCT GTCCAACTTG CCGTCCACAT CCGGTTCATA TGCTGAAGTT TAAATTCAAG 
AGGGGAAGTT TGCCACCCAT GATGCCTCTT GAATTTGTTG GATGCATCGG AGGATGTGAT 
GACACTCTCA GAGAGGTTTT AAACTTGAAA TATCTCAGAG GACGTGATGC AGAATCTGCC 
CCTCAGGCCA ATAACAGCTC CAGATCCAGT CAACACCACA TTCATAGAAC CAGCTCGGCA 
CCTCCTCCAT CCAGGGTAGA GAACATTAGA CCTCCAGCAC CCAGACCTCG AGCTGATCCT 
GGTCTGCAAC CTCCACCTTC AACTCAGGGC GGTGCCATTG TGTGTAACTG TGGTCAGGAC 
GCCTTACTTC TCACCGTGCG CAAAGAGGGT CCCAATCAAG GGCGTCAGTT TTACAAGTGT 
AACACAGGAG ACTGCAAGTT CTTCCTGTGG GCCGATCAGC CACCTGAACA GGGGGTGCCT 
GAAAGAAACA GAGGGCCTTT AAGTCAAAAC GTCCAGCCTC CCAGGCCTTC CAATGGGTTT 
GGGAATGTCT CACAGCAGAG GCAGAACCAC AGAGGGTCTG GAGGCGATGA AGGGGAAACT 
ATGTGTAACT GTAATGAGCC TTCAGTCACC AGGACTGTCA TGAAGGAAGG GCCGAACAAG 
GGCAGAATGT TTCACACCTG CGGGAAACCA CGAGATCAGC AGTGTGGCTT CTTCCAGTGG 
GCCAATGAAA ATGTGGCACC AGCCACAGCG TTTTCTGGAG GAAATGGTGG TAAACCATCT 
GGGAACAAAA AGGCCAGTAC TACCTCAGTC AAGCCTCCCA CTGCCAAAAG GCAGAGGACC 
TGTGGAATAT GTCATGAGCC AGGACACACC AGAGTCACCT GTCCTCAGGC CCACTGA