zak cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	zak
Entrez Gene ID	559247
Full Name	sterile alpha motif and leucine zipper containing kinase AZK
Synonyms	si:dkey-15i8.3
General protein information	Preferred Names sterile alpha motif and leucine zipper containing kinase AZK Names mitogen-activated protein kinase kinase kinase 20
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001327940.1	NP_001314869.1	mitogen-activated protein kinase kinase kinase 20

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Zak	NP_075544.1
Rattus norvegicus (Norway rat)	Zak	XP_006234431.1
Xenopus tropicalis (tropical clawed frog)	LOC100495797	XP_002934652.1
Homo sapiens (human)	ZAK	NP_057737.2
Danio rerio (zebrafish)	si:dkey-15i8.3	XP_687660.2
Pan troglodytes (chimpanzee)	LOC459745	XP_003309476.1
Bos taurus (cattle)	ZAK	NP_001192422.1
Gallus gallus (chicken)	ZAK	XP_421996.4
	ZAK	XP_001086798.2
Canis lupus familiaris (dog)	ZAK	XP_535966.2

Gene Ontology

The following zak gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the zak cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa14812	NM_001327940.1 Latest version!	Danio rerio sterile alpha motif and leucine zipper containing kinase AZK (zak), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

zak ( NM_001327940.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001314869.1 Danio rerio sterile alpha motif and leucine zipper containing kinase AZK (zak), mRNA.

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CloneID	ODa14812
Clone ID Related Accession (Same CDS sequence)	NM_001327940.1
Accession Version	NM_001327940.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-06
Organism	Danio rerio(zebrafish)
Product	mitogen-activated protein kinase kinase kinase 20
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX004769.9. On May 28, 2016 this sequence version replaced XM_682568.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because no single transcript was available for the full length of the gene. The extent of this transcript is supported by RNA-seq and orthologous data. ##Evidence-Data-START## CDS exon combination :: GFIL01036773.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCATCTC TGGGCAGCTC CTTTGTGCAG ATCAAGTTTG ATGACATCCA CTTCTATGAG 
AACTGCGGCG GCGGCAGCTT CGGGAGTGTG TATCGCGCCC GCTGGCTCTC GCAGGACAGA 
GAGGTGGCGG TGAAAAAACT GCTGAAGATC GAGAAAGAGG CTGAGATTTT GAGCGTTCTC 
AGTCATCGGA ACATTATCAA GTTTTATGGA GCGATTCTGG AAGCGCCCAA TTATGGGATT 
GTTACAGAGT ATGCCAGTGG CGGGTCTCTG TTTGACTATC TGTCCAGCGA CGACAGCGAG 
GACATCAGTA TGCAGCAGAT CATGACCTGG GCCATGGACA TCGCTAAAGG CATGCATTAT 
CTGCACTCTG AGGCCCCGGT TAAAGTCATT CACAGAGACC TCAAGTCCCG AAATGTTGTG 
TTAAGCTCAG ATTCAGTACT CAAGATCTGT GATTTCGGGG CGTCCAAGTT TCATTCACAC 
ACCACACACA TGTCTCTGGT GGGCACGTTT CCCTGGATGG CCCCTGAGGT CATTCAGAGC 
CTCCCGGTGT CTGAGACCTG CGACACCTTC TCCTACGGCG TGGTCCTGTG GGAGATGCTG 
ACTCAGGAGA TTCCCTTCAA AGGTCTGGAG GGGCTTCAGG TGGCCTGGCT GGTGGTGGAG 
AAACATGAGA GGTTGACGAT CCCCAGCAGC TGCCCAGCAA GTTTCGCCTG TTTGATGAGA 
AGCTGCTGGG CGACTGAACC AAAAGAGCGG CCGCTGTTTA AACACATCCT GTCCACACTG 
GAGTCCATGT GGAGCGACAC TCAGCTGCCT GACAAGTGCA ACACTTTCCT GCAGAATAAA 
GCCGAGTGGA GGTGTGAGAT CGAGGCGACT CTGGAGCGTC TGAAGCGTTT GGAAAGAGAT 
CTGAGCACCA AAGAACAAGC GCTGAAGGAG CGAGAGAGAC GCTTGAAGAT GTGGGAGAGG 
AAACTGATCG AGCAGTCAAA CACACCGCTT CTTCCAAACC TGGCCATTCA CTCCTGGACA 
GAAGAGCATG TGCATTTCTG GATGGAGCAG ATCTTTGAAG GTGACGGAGA GCTGCAGCAG 
TACGCCAATG TCTTCAGACA GAACCACATC ACAGGACAGA GACTGCTGCT GCTGTCCGAG 
TCTGACATGA GAGATCTGGG TGTGACGTCT AAAGGACACA TCATACACCT GAAGACTGAA 
ATCGAGAAAC TGACCCACGA CTACCTGAAT CTGTTTCACT TTCCTCCGCT GATCAAGGAC 
GGTTCGGCCT CTGAGGAAGC AGACGAGGAC TGGAGCAAGA CAGTGAACCT GGAGCTGGTG 
TTTGGGTATC ACTGGAAAGC AGGCACAGGA CAAAAGGACT GCAAATGGAA AATGTACATG 
GAGCTGGACG GGGACGAGGT GGCGATAGCG TACATCAAAG ACGTGACCTT TAACGCCAAC 
AGACCAGATG TAGATGTTTT AAAGATGACC AAGCCTCCTT TCGTGATGGA TAAATGGATA 
GTGGGAATAT CTGGCACACA TACTGTTGAC TACATAGTGA ACTATGAGGG TAACGTGAAA 
TCTCCGAAGT GTACAAGACA CGTTCACACA GTGCAGTGGA GTGCAGACAG CGGAAAAGAC 
GTGATCAAAC CTGTGGAGCT GGTCATCGAG ACGGCGCCGG GCATCAGGGA GGCGCACAAC 
AGGAGCAGGT CCAACTCAGG TGTGGATCCC AAATGGATTT ACAGTCTGAG AATGAAGCAG 
ATGAAAGAGC AGGCGGCCCA GGAGTCCCCC AGCAAAATGA GCCTCCCTCA GTTCCTGTCC 
GTCATCAGCA ACCAGTCTTC ATATGCGGCA GCTGTAAGAA GATCTCCAAA CCGCAGTCCT 
CTCAGTCCCT GGACCGACTC CCGCAGCTCC TCTCCAACTC TCTCCGTCAA ACTCTCCAAC 
ATGCACCTGG GCTCCAAAGG CAGCAGTCCG TCCAGCACCA CCTCCGAGAG CGCATCCGAA 
CGCGAGCGGC CCCCGAGCGC TGGAGCCAAA CACAACTACA ACAAAAAACC CTACTACGAT 
CACCACACAC TGAAGATGAG CAGTAGAGGA GATGGAGAAC CCAGGAGGTT TCTGCCGCAG 
AGAGGATCTA GAGGCTTCCA ACATGCTGGA CGACCACGAA ACGCACACTC ATACGCAACG 
CCACCGCACT ACCAAAGGCC CATACCGGGG ATGTCAATGG CCGCTGAAGG GCCGAAGCAG 
AAAGTGGCGT CTGAGGAACC AGGGGATGGG GACTGGATTA AAGTAGAGAG GAAAAAGACA 
AACAAACAGG AGTGTAAATA TCAGGAAGTG AGGCCGGTCA GAGGTCGGTC GCGTAGAGGT 
GGGAGAGGCG GTGGACGGGG GCGTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001314869.1
CDS	215..2584
Translation	MASLGSSFVQIKFDDIHFYENCGGGSFGSVYRARWLSQDREVAVKKLLKIEKEAEILSVLSHRNIIKFYGAILEAPNYGIVTEYASGGSLFDYLSSDDSEDISMQQIMTWAMDIAKGMHYLHSEAPVKVIHRDLKSRNVVLSSDSVLKICDFGASKFHSHTTHMSLVGTFPWMAPEVIQSLPVSETCDTFSYGVVLWEMLTQEIPFKGLEGLQVAWLVVEKHERLTIPSSCPASFACLMRSCWATEPKERPLFKHILSTLESMWSDTQLPDKCNTFLQNKAEWRCEIEATLERLKRLERDLSTKEQALKERERRLKMWERKLIEQSNTPLLPNLAIHSWTEEHVHFWMEQIFEGDGELQQYANVFRQNHITGQRLLLLSESDMRDLGVTSKGHIIHLKTEIEKLTHDYLNLFHFPPLIKDGSASEEADEDWSKTVNLELVFGYHWKAGTGQKDCKWKMYMELDGDEVAIAYIKDVTFNANRPDVDVLKMTKPPFVMDKWIVGISGTHTVDYIVNYEGNVKSPKCTRHVHTVQWSADSGKDVIKPVELVIETAPGIREAHNRSRSNSGVDPKWIYSLRMKQMKEQAAQESPSKMSLPQFLSVISNQSSYAAAVRRSPNRSPLSPWTDSRSSSPTLSVKLSNMHLGSKGSSPSSTTSESASERERPPSAGAKHNYNKKPYYDHHTLKMSSRGDGEPRRFLPQRGSRGFQHAGRPRNAHSYATPPHYQRPIPGMSMAAEGPKQKVASEEPGDGDWIKVERKKTNKQECKYQEVRPVRGRSRRGGRGGGRGRN

Target ORF information:

RefSeq Version	NM_001327940.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sterile alpha motif and leucine zipper containing kinase AZK (zak), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001327940.1

ORF Insert Sequence:

ATGGCATCTC TGGGCAGCTC CTTTGTGCAG ATCAAGTTTG ATGACATCCA CTTCTATGAG 
AACTGCGGCG GCGGCAGCTT CGGGAGTGTG TATCGCGCCC GCTGGCTCTC GCAGGACAGA 
GAGGTGGCGG TGAAAAAACT GCTGAAGATC GAGAAAGAGG CTGAGATTTT GAGCGTTCTC 
AGTCATCGGA ACATTATCAA GTTTTATGGA GCGATTCTGG AAGCGCCCAA TTATGGGATT 
GTTACAGAGT ATGCCAGTGG CGGGTCTCTG TTTGACTATC TGTCCAGCGA CGACAGCGAG 
GACATCAGTA TGCAGCAGAT CATGACCTGG GCCATGGACA TCGCTAAAGG CATGCATTAT 
CTGCACTCTG AGGCCCCGGT TAAAGTCATT CACAGAGACC TCAAGTCCCG AAATGTTGTG 
TTAAGCTCAG ATTCAGTACT CAAGATCTGT GATTTCGGGG CGTCCAAGTT TCATTCACAC 
ACCACACACA TGTCTCTGGT GGGCACGTTT CCCTGGATGG CCCCTGAGGT CATTCAGAGC 
CTCCCGGTGT CTGAGACCTG CGACACCTTC TCCTACGGCG TGGTCCTGTG GGAGATGCTG 
ACTCAGGAGA TTCCCTTCAA AGGTCTGGAG GGGCTTCAGG TGGCCTGGCT GGTGGTGGAG 
AAACATGAGA GGTTGACGAT CCCCAGCAGC TGCCCAGCAA GTTTCGCCTG TTTGATGAGA 
AGCTGCTGGG CGACTGAACC AAAAGAGCGG CCGCTGTTTA AACACATCCT GTCCACACTG 
GAGTCCATGT GGAGCGACAC TCAGCTGCCT GACAAGTGCA ACACTTTCCT GCAGAATAAA 
GCCGAGTGGA GGTGTGAGAT CGAGGCGACT CTGGAGCGTC TGAAGCGTTT GGAAAGAGAT 
CTGAGCACCA AAGAACAAGC GCTGAAGGAG CGAGAGAGAC GCTTGAAGAT GTGGGAGAGG 
AAACTGATCG AGCAGTCAAA CACACCGCTT CTTCCAAACC TGGCCATTCA CTCCTGGACA 
GAAGAGCATG TGCATTTCTG GATGGAGCAG ATCTTTGAAG GTGACGGAGA GCTGCAGCAG 
TACGCCAATG TCTTCAGACA GAACCACATC ACAGGACAGA GACTGCTGCT GCTGTCCGAG 
TCTGACATGA GAGATCTGGG TGTGACGTCT AAAGGACACA TCATACACCT GAAGACTGAA 
ATCGAGAAAC TGACCCACGA CTACCTGAAT CTGTTTCACT TTCCTCCGCT GATCAAGGAC 
GGTTCGGCCT CTGAGGAAGC AGACGAGGAC TGGAGCAAGA CAGTGAACCT GGAGCTGGTG 
TTTGGGTATC ACTGGAAAGC AGGCACAGGA CAAAAGGACT GCAAATGGAA AATGTACATG 
GAGCTGGACG GGGACGAGGT GGCGATAGCG TACATCAAAG ACGTGACCTT TAACGCCAAC 
AGACCAGATG TAGATGTTTT AAAGATGACC AAGCCTCCTT TCGTGATGGA TAAATGGATA 
GTGGGAATAT CTGGCACACA TACTGTTGAC TACATAGTGA ACTATGAGGG TAACGTGAAA 
TCTCCGAAGT GTACAAGACA CGTTCACACA GTGCAGTGGA GTGCAGACAG CGGAAAAGAC 
GTGATCAAAC CTGTGGAGCT GGTCATCGAG ACGGCGCCGG GCATCAGGGA GGCGCACAAC 
AGGAGCAGGT CCAACTCAGG TGTGGATCCC AAATGGATTT ACAGTCTGAG AATGAAGCAG 
ATGAAAGAGC AGGCGGCCCA GGAGTCCCCC AGCAAAATGA GCCTCCCTCA GTTCCTGTCC 
GTCATCAGCA ACCAGTCTTC ATATGCGGCA GCTGTAAGAA GATCTCCAAA CCGCAGTCCT 
CTCAGTCCCT GGACCGACTC CCGCAGCTCC TCTCCAACTC TCTCCGTCAA ACTCTCCAAC 
ATGCACCTGG GCTCCAAAGG CAGCAGTCCG TCCAGCACCA CCTCCGAGAG CGCATCCGAA 
CGCGAGCGGC CCCCGAGCGC TGGAGCCAAA CACAACTACA ACAAAAAACC CTACTACGAT 
CACCACACAC TGAAGATGAG CAGTAGAGGA GATGGAGAAC CCAGGAGGTT TCTGCCGCAG 
AGAGGATCTA GAGGCTTCCA ACATGCTGGA CGACCACGAA ACGCACACTC ATACGCAACG 
CCACCGCACT ACCAAAGGCC CATACCGGGG ATGTCAATGG CCGCTGAAGG GCCGAAGCAG 
AAAGTGGCGT CTGAGGAACC AGGGGATGGG GACTGGATTA AAGTAGAGAG GAAAAAGACA 
AACAAACAGG AGTGTAAATA TCAGGAAGTG AGGCCGGTCA GAGGTCGGTC GCGTAGAGGT 
GGGAGAGGCG GTGGACGGGG GCGTAACTGA