amotl2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	amotl2a
Entrez Gene ID	558920
Full Name	angiomotin like 2a
Synonyms	amotl2,cb308,sb:cb308,wu:fc41b07,wu:fc64c06
General protein information	Preferred Names angiomotin like 2a Names angiomotin-like 2a angiomotin like 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001080177.1	NP_001073646.1	angiomotin-like 2a

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AMOTL2	XP_003310087.1
Mus musculus (house mouse)	Amotl2	NP_062738.2
Gallus gallus (chicken)	AMOTL2	XP_004937049.1
Xenopus tropicalis (tropical clawed frog)	amotl2	XP_002935132.2
Homo sapiens (human)	AMOTL2	NP_057285.3
Canis lupus familiaris (dog)	AMOTL2	XP_542789.3
Danio rerio (zebrafish)	amotl2a	NP_001073646.1
	AMOTL2	XP_001112164.2
Bos taurus (cattle)	AMOTL2	XP_005201993.1
Rattus norvegicus (Norway rat)	Amotl2	NP_113905.1

Gene Ontology
Bibliography

Related articles in PubMed

The E-cadherin/AmotL2 complex organizes actin filaments required for epithelial hexagonal packing and blastocyst hatching.
Hildebrand S, Hultin S, Subramani A, Petropoulos S, Zhang Y, Cao X, Mpindi J, Kalloniemi O, Johansson S, Majumdar A, Lanner F, Holmgren L
Scientific reports7(1)9540(2017 Aug)

AmotL2 integrates polarity and junctional cues to modulate cell shape.
Hultin S, Subramani A, Hildebrand S, Zheng Y, Majumdar A, Holmgren L
Scientific reports7(1)7548(2017 Aug)

Flow-Dependent Endothelial YAP Regulation Contributes to Vessel Maintenance.
Nakajima H, Yamamoto K, Agarwala S, Terai K, Fukui H, Fukuhara S, Ando K, Miyazaki T, Yokota Y, Schmelzer E, Belting HG, Affolter M, Lecaudey V, Mochizuki N
Developmental cell40(6)523-536.e6(2017 03)

Proliferation-independent regulation of organ size by Fgf/Notch signaling.
Kozlovskaja-Gumbrien? A, Yi R, Alexander R, Aman A, Jiskra R, Nagelberg D, Knaut H, McClain M, Piotrowski T
eLife6(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Amotl2a function in the control of lateral line primordium cell proliferation is mediated together by the Hippo pathway effector Yap1 and the Wnt/beta-catenin effector Lef1.
Title: Amotl2a interacts with the Hippo effector Yap1 and the Wnt/?-catenin effector Lef1 to control tissue size in zebrafish.

amotL2 associates to the VE-cadherin adhesion complex where it couples adherens junctions to contractile actin fibres.
Title: AmotL2 links VE-cadherin to contractile actin fibres necessary for aortic lumen expansion.

We demonstrate that amotl2 knockdown in zebrafish wild-type embryos results in embryonic dorsalization, and this effect can be antagonized by co-knockdown of the dorsal inducer beta-catenin2.
Title: The Amotl2 gene inhibits Wnt/?-catenin signaling and regulates embryonic development in zebrafish.

Amotl2 plays a pivotal role in polarity, migration and proliferation of angiogenic endothelial cells
Title: Angiomotin-like2 gene (amotl2) is required for migration and proliferation of endothelial cells during angiogenesis.

Amotl2 interacts preferentially with and facilitates translocation of the phosphorylated c-Src, which may in turn regulate the membrane architecture. These data provide the first evidence that amotl2 is essential for cell movements in vertebrate embryos.
Title: Amotl2 is essential for cell movements in zebrafish embryo and regulates c-Src translocation.

The following amotl2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the amotl2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00872	NM_001080177.1 Latest version!	Danio rerio angiomotin like 2a (amotl2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

amotl2a ( NM_001080177.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001073646.1 Danio rerio angiomotin like 2a (amotl2a), mRNA.

Price & Availability↑

CloneID	ODa00872
Clone ID Related Accession (Same CDS sequence)	NM_001080177.1
Accession Version	NM_001080177.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Danio rerio(zebrafish)
Product	angiomotin-like 2a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ887096.1. On Mar 11, 2007 this sequence version replaced XM_702236.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ887096.1, BC163299.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGAACGG CAGAGGAATC ATCAGGAACG GTGCTGCACC GACTGATTCA AGAGCAGCTT 
CGTTATGGAA ACCCTACCGA TCCTACTCTG TTAGCCATCC AGCAGCAGGC TCTGCGTGGA 
GGCAGCAGCG GTGGAGGTGC AGGAAGCCCT CGTTCATCCT TGGAAAGCCT AACACAAGAG 
GAATCACTTT CTCCCCAGTT GTCCGCCCGG CAAGAGCCCC AGGGCCAAGA GCACCAGGGT 
GACTTCCAGC ATTCAGAGAG TCCTGTTTGT CACCTTTATC AGCTCCACAC AGAGGAGCTG 
CCTACTTATG AGGAAGCAAA GGCACACTCA CAGTATCTTG CCTACCAGAG AGGACAGATA 
GGGCTTCATC ACGGCAGTCT GGAGAGCCCT GGAGGGGTTG GAGGAGCCGA ACAGGACGAC 
AGCATGTGGG ATGCGAAACG AGAGCATGCA CGCTCACTAA GTGAGCGTCT TTTGCAACTC 
TCACTAGAGA GAAATTGTGC CCATGACAAT ATTCCCATGA GCTCATCGCA TAGTTATCCC 
CAACTCTCTA ATAACCACTC TGATACAGTT GTGAATGAGC AGTCAGTTCA TCAGCCAGAC 
CAACGGGGTC CTCCCCCGGA GTACCCCTTC ATGGTCAGAT CCCCTGGATA TATGCTTAGT 
CATTCACAGG AACATGGACA CTATTATAAT GAGCCCCCTC CTGCCTTCCA TTCACAGCAT 
TACAGGCTAT TTCCAACACA GCCACAAGCA CCTCGTCACA ATGGCCTGCC CACATTGACC 
CCTGCTGGCC AGGATGTCAA TGTTGGAGGA TACAGCATCC CAGCGAACAA CTTCCAAATG 
GAGCAGCTTA TTAAAGAGAA CGAAAGACTG AAAAGAGAAG TGGACAGCTA CAGTGAGAAG 
GCGGCAAGGT TACAGAAGCT TGAACAGGAG ATTCAGAGGA TATCGGAGGC CTATGAGACT 
CTTATGAAGG GATCTGCAAA GAGAGAAGCC CTTGAGAAAA CCATGAGGAA CAAGCTAGAG 
AGTGAGATCA AGCGACTTCA TGACTTTAAC AGAGATCTCA GGGACCGCCT GGAAACAGCC 
AACAAACAGA GAGCAGCCAT TGAAGTTGAA GACAAGAGTC GGCATGCCTT CGCCAAACTG 
GTTGAGCAAA ATGAGGACCA TCTTCGAGAG CGGGAACGTC TGGAAAAAGA GACACAGCAT 
CTAAGGGCCT CAGGAGAAGA ATGGAAGAGG CGTCGTGAAG CCTTGGAGCA AGCACTTATC 
ACAGCTCAGA CACGAAATAG GCAGCTGGAA GAAGAGTTAC GTAGGAAGAG AGCTTATGTG 
GAGAAGGTGG AAAGGATGCA GAGTGCATTG GCGCAATTGC AGGCAGCATG TGAGAAGAGG 
GAAGCACTGG AGTTACGACT TAGAACCAGA CTGGAGCAGG AGCTGAAGAG TTTGAGAGCA 
CAGCAGTGGC AGGCCCAAAC CCAGCATGCA AGCCCAGGGT CATACTTGGA CCTTAATGTA 
TCATCACTAC AGCAGCAACT GAGGGAGAGA GAGGAGCAGG TTTTGGCCCT GGAGGCTGAC 
ATCACCCGCT GGGAGCAGAA GTATCTTGAG GAGAGCACAA TGCGTCAGTT TGCCATGGAT 
GCTGCTGCTA CTGCCGCAGC TCAGAGGGAT ACCACAATCA TCAACCATTC ACCCCGCAAT 
TCACCCAACA GCAGCTTCAA TGAGGATTTG CCGTCCCCCA ACCACAGGCA TCAGGAGATG 
GAAAACCGGA TTCGGGCACT TTATGCTCAA CTCTTGGAGA AGGATGCCAT TATAAAGGTC 
ATGCAGCAGC GGTCAAGACG AGAGCAAGGC CGACCTGAGT TACAAGGCTT ACGACCTGCT 
CGATCTGTTC CCTCCATCAA CACCGTTGCC ACAGCTAGCA CCACCCGAGC CAAAGGGAAG 
AGCCTATCAG ATGACCAGAC AGCAGTCGCA TCTTTGCCCC CATTGCCCCA TCTGCTTGCC 
AAAATCCAGT GTCGAGACAG CAGCACACAG TGTGATTCAG AAGAGCCCAG CTGTAAAGCA 
GAACCTGCTG ATGTTGCTGT ATCTGCTCCA GAACCTTCCA CTGCTTCTAG CTCTGAATCA 
ACCTCTCTCA AGACAACACA GATCAGCAGT GCCGTCGAAA ATGACATGGT GGAGATCCTC 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073646.1
CDS	216..2381
Translation	MRTAEESSGTVLHRLIQEQLRYGNPTDPTLLAIQQQALRGGSSGGGAGSPRSSLESLTQEESLSPQLSARQEPQGQEHQGDFQHSESPVCHLYQLHTEELPTYEEAKAHSQYLAYQRGQIGLHHGSLESPGGVGGAEQDDSMWDAKREHARSLSERLLQLSLERNCAHDNIPMSSSHSYPQLSNNHSDTVVNEQSVHQPDQRGPPPEYPFMVRSPGYMLSHSQEHGHYYNEPPPAFHSQHYRLFPTQPQAPRHNGLPTLTPAGQDVNVGGYSIPANNFQMEQLIKENERLKREVDSYSEKAARLQKLEQEIQRISEAYETLMKGSAKREALEKTMRNKLESEIKRLHDFNRDLRDRLETANKQRAAIEVEDKSRHAFAKLVEQNEDHLRERERLEKETQHLRASGEEWKRRREALEQALITAQTRNRQLEEELRRKRAYVEKVERMQSALAQLQAACEKREALELRLRTRLEQELKSLRAQQWQAQTQHASPGSYLDLNVSSLQQQLREREEQVLALEADITRWEQKYLEESTMRQFAMDAAATAAAQRDTTIINHSPRNSPNSSFNEDLPSPNHRHQEMENRIRALYAQLLEKDAIIKVMQQRSRREQGRPELQGLRPARSVPSINTVATASTTRAKGKSLSDDQTAVASLPPLPHLLAKIQCRDSSTQCDSEEPSCKAEPADVAVSAPEPSTASSSESTSLKTTQISSAVENDMVEILI

Target ORF information:

RefSeq Version	NM_001080177.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio angiomotin like 2a (amotl2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080177.1

ORF Insert Sequence:

ATGAGAACGG CAGAGGAATC ATCAGGAACG GTGCTGCACC GACTGATTCA AGAGCAGCTT 
CGTTATGGAA ACCCTACCGA TCCTACTCTG TTAGCCATCC AGCAGCAGGC TCTGCGTGGA 
GGCAGCAGCG GTGGAGGTGC AGGAAGCCCT CGTTCATCCT TGGAAAGCCT AACACAAGAG 
GAATCACTTT CTCCCCAGTT GTCCGCCCGG CAAGAGCCCC AGGGCCAAGA GCACCAGGGT 
GACTTCCAGC ATTCAGAGAG TCCTGTTTGT CACCTTTATC AGCTCCACAC AGAGGAGCTG 
CCTACTTATG AGGAAGCAAA GGCACACTCA CAGTATCTTG CCTACCAGAG AGGACAGATA 
GGGCTTCATC ACGGCAGTCT GGAGAGCCCT GGAGGGGTTG GAGGAGCCGA ACAGGACGAC 
AGCATGTGGG ATGCGAAACG AGAGCATGCA CGCTCACTAA GTGAGCGTCT TTTGCAACTC 
TCACTAGAGA GAAATTGTGC CCATGACAAT ATTCCCATGA GCTCATCGCA TAGTTATCCC 
CAACTCTCTA ATAACCACTC TGATACAGTT GTGAATGAGC AGTCAGTTCA TCAGCCAGAC 
CAACGGGGTC CTCCCCCGGA GTACCCCTTC ATGGTCAGAT CCCCTGGATA TATGCTTAGT 
CATTCACAGG AACATGGACA CTATTATAAT GAGCCCCCTC CTGCCTTCCA TTCACAGCAT 
TACAGGCTAT TTCCAACACA GCCACAAGCA CCTCGTCACA ATGGCCTGCC CACATTGACC 
CCTGCTGGCC AGGATGTCAA TGTTGGAGGA TACAGCATCC CAGCGAACAA CTTCCAAATG 
GAGCAGCTTA TTAAAGAGAA CGAAAGACTG AAAAGAGAAG TGGACAGCTA CAGTGAGAAG 
GCGGCAAGGT TACAGAAGCT TGAACAGGAG ATTCAGAGGA TATCGGAGGC CTATGAGACT 
CTTATGAAGG GATCTGCAAA GAGAGAAGCC CTTGAGAAAA CCATGAGGAA CAAGCTAGAG 
AGTGAGATCA AGCGACTTCA TGACTTTAAC AGAGATCTCA GGGACCGCCT GGAAACAGCC 
AACAAACAGA GAGCAGCCAT TGAAGTTGAA GACAAGAGTC GGCATGCCTT CGCCAAACTG 
GTTGAGCAAA ATGAGGACCA TCTTCGAGAG CGGGAACGTC TGGAAAAAGA GACACAGCAT 
CTAAGGGCCT CAGGAGAAGA ATGGAAGAGG CGTCGTGAAG CCTTGGAGCA AGCACTTATC 
ACAGCTCAGA CACGAAATAG GCAGCTGGAA GAAGAGTTAC GTAGGAAGAG AGCTTATGTG 
GAGAAGGTGG AAAGGATGCA GAGTGCATTG GCGCAATTGC AGGCAGCATG TGAGAAGAGG 
GAAGCACTGG AGTTACGACT TAGAACCAGA CTGGAGCAGG AGCTGAAGAG TTTGAGAGCA 
CAGCAGTGGC AGGCCCAAAC CCAGCATGCA AGCCCAGGGT CATACTTGGA CCTTAATGTA 
TCATCACTAC AGCAGCAACT GAGGGAGAGA GAGGAGCAGG TTTTGGCCCT GGAGGCTGAC 
ATCACCCGCT GGGAGCAGAA GTATCTTGAG GAGAGCACAA TGCGTCAGTT TGCCATGGAT 
GCTGCTGCTA CTGCCGCAGC TCAGAGGGAT ACCACAATCA TCAACCATTC ACCCCGCAAT 
TCACCCAACA GCAGCTTCAA TGAGGATTTG CCGTCCCCCA ACCACAGGCA TCAGGAGATG 
GAAAACCGGA TTCGGGCACT TTATGCTCAA CTCTTGGAGA AGGATGCCAT TATAAAGGTC 
ATGCAGCAGC GGTCAAGACG AGAGCAAGGC CGACCTGAGT TACAAGGCTT ACGACCTGCT 
CGATCTGTTC CCTCCATCAA CACCGTTGCC ACAGCTAGCA CCACCCGAGC CAAAGGGAAG 
AGCCTATCAG ATGACCAGAC AGCAGTCGCA TCTTTGCCCC CATTGCCCCA TCTGCTTGCC 
AAAATCCAGT GTCGAGACAG CAGCACACAG TGTGATTCAG AAGAGCCCAG CTGTAAAGCA 
GAACCTGCTG ATGTTGCTGT ATCTGCTCCA GAACCTTCCA CTGCTTCTAG CTCTGAATCA 
ACCTCTCTCA AGACAACACA GATCAGCAGT GCCGTCGAAA ATGACATGGT GGAGATCCTC 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The E-cadherin/AmotL2 complex organizes actin filaments required for epithelial hexagonal packing and blastocyst hatching.
Scientific reports7(1)9540(2017 Aug)
Hildebrand S,Hultin S,Subramani A,Petropoulos S,Zhang Y,Cao X,Mpindi J,Kalloniemi O,Johansson S,Majumdar A,Lanner F,Holmgren L

AmotL2 integrates polarity and junctional cues to modulate cell shape.
Scientific reports7(1)7548(2017 Aug)
Hultin S,Subramani A,Hildebrand S,Zheng Y,Majumdar A,Holmgren L

Flow-Dependent Endothelial YAP Regulation Contributes to Vessel Maintenance.
Developmental cell40(6)523-536.e6(2017 03)
Nakajima H,Yamamoto K,Agarwala S,Terai K,Fukui H,Fukuhara S,Ando K,Miyazaki T,Yokota Y,Schmelzer E,Belting HG,Affolter M,Lecaudey V,Mochizuki N

Proliferation-independent regulation of organ size by Fgf/Notch signaling.
eLife6(2017 Jan)
Kozlovskaja-Gumbrien? A,Yi R,Alexander R,Aman A,Jiskra R,Nagelberg D,Knaut H,McClain M,Piotrowski T

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amotl2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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