gpc1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gpc1a
Entrez Gene ID	553367
Full Name	glypican 1a
Synonyms	Gpc1,si:rp71-69p9.1,zgc:122977
General protein information	Preferred Names glypican 1a Names glypican-1 heparan sulfate proteoglycan
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001037373.1	NP_001032450.1	glypican-1 precursor

Pathways from BioSystems

Homologies

	GPC1	XP_001089255.1
Xenopus tropicalis (tropical clawed frog)	gpc1	NP_001072316.1
Homo sapiens (human)	GPC1	NP_002072.2
Pan troglodytes (chimpanzee)	GPC1	XP_001156197.2
Danio rerio (zebrafish)	gpc1a	NP_001032450.1
Canis lupus familiaris (dog)	GPC1	XP_005636000.1
Gallus gallus (chicken)	GPC1	XP_422590.2
Danio rerio (zebrafish)	gpc1b	NP_957314.1
Bos taurus (cattle)	GPC1	NP_001039701.1
Mus musculus (house mouse)	Gpc1	NP_057905.1
Rattus norvegicus (Norway rat)	Gpc1	NP_110455.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Loss of a Candidate Biliary Atresia Susceptibility Gene, add3a, Causes Biliary Developmental Defects in Zebrafish.
Tang V, Cofer ZC, Cui S, Sapp V, Loomes KM, Matthews RP
Journal of pediatric gastroenterology and nutrition63(5)524-530(2016 Nov)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Identification and expression analysis of zebrafish glypicans during embryonic development.
Gupta M, Brand M
PloS one8(11)e80824(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gpc1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gpc1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01857	NM_001037373.1 Latest version!	Danio rerio glypican 1a (gpc1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gpc1a ( NM_001037373.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001032450.1 Danio rerio glypican 1a (gpc1a), mRNA.

Price & Availability↑

CloneID	ODa01857
Clone ID Related Accession (Same CDS sequence)	NM_001037373.1
Accession Version	NM_001037373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Danio rerio(zebrafish)
Product	glypican-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC109411.1. On Nov 26, 2005 this sequence version replaced XM_692398.1. ##Evidence-Data-START## Transcript exon combination :: BC109411.1, BC092886.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGATCTGA CAGCGGTCGC GCTGCTCGTG TCGCTCGTGT CCGTGTCTCT GTCCGCGGAG 
AACGCGGGTG GTAAAGCGCG GAGCTGCACG GACGTGCGCC AGTTCTACAG CGGCAAAGGC 
TTCACGTTAA ACGGCGTCCC GCAGTCGGAG ATCTCAGGAG AGCATCTCCG CATCTGCCCG 
CAAGGTTACA CCTGCTGCAC CAGTGCCATG GAGGAAACGC TGTCGAACCT GAGCCGCAGA 
GAATTTGAAG GCCTGGTCAG AGAAGCAGGA CGATCCATTC AAGCTTTGCT CAATGCTCAG 
TACAGAACCT TTGACACATA TTTCCTGGAA CTCCTGGATG GCTCTGAACG GTGGTTAGAG 
GAGGCTTTTG TGGCTGCACT TGGAGAACTG TATCGACTGA ATGCAGGAGT GTTTCGTGAC 
CTCTATGCCG AACTGCATCG ATATTACAGT GGTGCTTCCC TGAATCTCGA GGAGGCCTTG 
GATGAGTTCT GGATGAAGCT CCTGGAGCGA CTTCTGAAGG CGTCTGACCC TGAAACTGCC 
TCCCTGCTCT CTGATGACTT CCTTGATTGT GCCTCCAAGC AGACCGAGAC CCTGCGTCCT 
TTTGGAGATG CTCCACGGGA ACTAAAGGCC AAGCTGGTGC GGGCATTCAT TGCGGCTCGG 
GCATTTGTCC AGGGCCTTAA CGCAGCTGGA GAGATTGTAC GGAAGGTGTC TCAGGTCCCA 
CTGAGCCCAG AGTGCAATCG TGCCATTATG AAACTGGTGT ACTGCCCTCA TTGCAGAGGT 
CTGGGATCAG TCAAACCTTG CATCAACTAC TGCAAAAATG TGATGAAGGG CTGCCTGGCT 
AATCAGGCTG ACCTGGACAC TGAGTGGCAG AGCCTCATAG AAACCATGCT ACAGGTGGCC 
TCCAGTTTTG GTGCCGAGCC CAGTATGGAC ACGGTGATCT ATTCAATTCC TGTGAGAATC 
TCTGAAGCTG TCCTCGCCAT GCAGGAGAAC ATGGAGATCT ATACCAGCAA GGTTTTTAAG 
GCCTGTGGGG ACCGTGGTGA GGAAGGCACA CCAAGTTCAA TATCTGAGGA GCCAAAAAAG 
AAAGAACGCA CAGTGACTGC TCTGGAATAC AAACCCAGCC CTAAGTCTGC AGCCAGACTG 
GAGGTCCAGG TCACGGATGT GTACAGCAAA CTCAAGGAAA TGCAGCTTTA CTGGATCCAA 
CTTCCTTCGG CTTTGTGCAG TGGTAAAACG GCATCTTCAA CCACTGGTGA CAAATGCTGG 
AATGGCATCA CCAAAGCCAG TTACCTCCCA GAGGTCATGG GTGATGGGCT GGCCAATCAG 
ATTAATAACC CAGAGGTGGA GATCGACATC ACTAAGCCTG ACAGGACTAT ACGTCAACAG 
ATCATGCTGC TGAAGATCAT GTCTAACCGC CTGAAAAACG CCCTCGAAGG CAATGATGTG 
GATTTCCAAG ATACAAGTGA TGATTTCAGT GGCTCGGGAA GCGGCATGTG TGCGGATCAC 
CTGTGTGTTC GTGGTCGACC TCCGGTTTTT GGGCCGAAAA CGGACAGGCC CAAACTCTAT 
GCCACTGGGC CTGAAAACAA AAGAGTCAAA GGATCCGGGA ACCAGATTCA GCCTTCTTTC 
ATTTTACTGA TTGTGTTCTT TGTGAGTCTA TTGCTCAGAC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032450.1
CDS	408..2072
Translation	MDLTAVALLVSLVSVSLSAENAGGKARSCTDVRQFYSGKGFTLNGVPQSEISGEHLRICPQGYTCCTSAMEETLSNLSRREFEGLVREAGRSIQALLNAQYRTFDTYFLELLDGSERWLEEAFVAALGELYRLNAGVFRDLYAELHRYYSGASLNLEEALDEFWMKLLERLLKASDPETASLLSDDFLDCASKQTETLRPFGDAPRELKAKLVRAFIAARAFVQGLNAAGEIVRKVSQVPLSPECNRAIMKLVYCPHCRGLGSVKPCINYCKNVMKGCLANQADLDTEWQSLIETMLQVASSFGAEPSMDTVIYSIPVRISEAVLAMQENMEIYTSKVFKACGDRGEEGTPSSISEEPKKKERTVTALEYKPSPKSAARLEVQVTDVYSKLKEMQLYWIQLPSALCSGKTASSTTGDKCWNGITKASYLPEVMGDGLANQINNPEVEIDITKPDRTIRQQIMLLKIMSNRLKNALEGNDVDFQDTSDDFSGSGSGMCADHLCVRGRPPVFGPKTDRPKLYATGPENKRVKGSGNQIQPSFILLIVFFVSLLLRR

Target ORF information:

RefSeq Version	NM_001037373.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glypican 1a (gpc1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037373.1

ORF Insert Sequence:

ATGGATCTGA CAGCGGTCGC GCTGCTCGTG TCGCTCGTGT CCGTGTCTCT GTCCGCGGAG 
AACGCGGGTG GTAAAGCGCG GAGCTGCACG GACGTGCGCC AGTTCTACAG CGGCAAAGGC 
TTCACGTTAA ACGGCGTCCC GCAGTCGGAG ATCTCAGGAG AGCATCTCCG CATCTGCCCG 
CAAGGTTACA CCTGCTGCAC CAGTGCCATG GAGGAAACGC TGTCGAACCT GAGCCGCAGA 
GAATTTGAAG GCCTGGTCAG AGAAGCAGGA CGATCCATTC AAGCTTTGCT CAATGCTCAG 
TACAGAACCT TTGACACATA TTTCCTGGAA CTCCTGGATG GCTCTGAACG GTGGTTAGAG 
GAGGCTTTTG TGGCTGCACT TGGAGAACTG TATCGACTGA ATGCAGGAGT GTTTCGTGAC 
CTCTATGCCG AACTGCATCG ATATTACAGT GGTGCTTCCC TGAATCTCGA GGAGGCCTTG 
GATGAGTTCT GGATGAAGCT CCTGGAGCGA CTTCTGAAGG CGTCTGACCC TGAAACTGCC 
TCCCTGCTCT CTGATGACTT CCTTGATTGT GCCTCCAAGC AGACCGAGAC CCTGCGTCCT 
TTTGGAGATG CTCCACGGGA ACTAAAGGCC AAGCTGGTGC GGGCATTCAT TGCGGCTCGG 
GCATTTGTCC AGGGCCTTAA CGCAGCTGGA GAGATTGTAC GGAAGGTGTC TCAGGTCCCA 
CTGAGCCCAG AGTGCAATCG TGCCATTATG AAACTGGTGT ACTGCCCTCA TTGCAGAGGT 
CTGGGATCAG TCAAACCTTG CATCAACTAC TGCAAAAATG TGATGAAGGG CTGCCTGGCT 
AATCAGGCTG ACCTGGACAC TGAGTGGCAG AGCCTCATAG AAACCATGCT ACAGGTGGCC 
TCCAGTTTTG GTGCCGAGCC CAGTATGGAC ACGGTGATCT ATTCAATTCC TGTGAGAATC 
TCTGAAGCTG TCCTCGCCAT GCAGGAGAAC ATGGAGATCT ATACCAGCAA GGTTTTTAAG 
GCCTGTGGGG ACCGTGGTGA GGAAGGCACA CCAAGTTCAA TATCTGAGGA GCCAAAAAAG 
AAAGAACGCA CAGTGACTGC TCTGGAATAC AAACCCAGCC CTAAGTCTGC AGCCAGACTG 
GAGGTCCAGG TCACGGATGT GTACAGCAAA CTCAAGGAAA TGCAGCTTTA CTGGATCCAA 
CTTCCTTCGG CTTTGTGCAG TGGTAAAACG GCATCTTCAA CCACTGGTGA CAAATGCTGG 
AATGGCATCA CCAAAGCCAG TTACCTCCCA GAGGTCATGG GTGATGGGCT GGCCAATCAG 
ATTAATAACC CAGAGGTGGA GATCGACATC ACTAAGCCTG ACAGGACTAT ACGTCAACAG 
ATCATGCTGC TGAAGATCAT GTCTAACCGC CTGAAAAACG CCCTCGAAGG CAATGATGTG 
GATTTCCAAG ATACAAGTGA TGATTTCAGT GGCTCGGGAA GCGGCATGTG TGCGGATCAC 
CTGTGTGTTC GTGGTCGACC TCCGGTTTTT GGGCCGAAAA CGGACAGGCC CAAACTCTAT 
GCCACTGGGC CTGAAAACAA AAGAGTCAAA GGATCCGGGA ACCAGATTCA GCCTTCTTTC 
ATTTTACTGA TTGTGTTCTT TGTGAGTCTA TTGCTCAGAC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Loss of a Candidate Biliary Atresia Susceptibility Gene, add3a, Causes Biliary Developmental Defects in Zebrafish.
Journal of pediatric gastroenterology and nutrition63(5)524-530(2016 Nov)
Tang V,Cofer ZC,Cui S,Sapp V,Loomes KM,Matthews RP

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Identification and expression analysis of zebrafish glypicans during embryonic development.
PloS one8(11)e80824(2013)
Gupta M,Brand M