lats1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lats1
Entrez Gene ID	553164
Full Name	large tumor suppressor kinase 1
Synonyms	Lats,si:dkey-181i3.5,wu:fc19a11,zgc:123219
General protein information	Preferred Names large tumor suppressor kinase 1 Names serine/threonine-protein kinase LATS1 LATS, large tumor suppressor, homolog 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005160312.4	XP_005160369.1	serine/threonine-protein kinase LATS1 isoform X1
XM_005160312.3	XP_005160369.1	serine/threonine-protein kinase LATS1 isoform X1
XM_021468417.1	XP_021324092.1	serine/threonine-protein kinase LATS1 isoform X2
XM_009294504.2	XP_009292779.1	serine/threonine-protein kinase LATS1 isoform X2
NM_001020510.1	NP_001018346.1	serine/threonine-protein kinase LATS1
XM_009294504.3	XP_009292779.1	serine/threonine-protein kinase LATS1 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	LATS1	NP_004681.1
Bos taurus (cattle)	LATS1	NP_001179795.1
Rattus norvegicus (Norway rat)	Lats1	NP_001128015.2
Gallus gallus (chicken)	LATS1	XP_004935664.1
Pan troglodytes (chimpanzee)	LATS1	XP_001173355.1
	LOC696131	XP_002808442.1
Mus musculus (house mouse)	Lats1	NP_034820.1
Danio rerio (zebrafish)	lats1	NP_001018346.1
Xenopus tropicalis (tropical clawed frog)	lats1	XP_002943506.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Phosphorylation of angiomotin by Lats1/2 kinases inhibits F-actin binding, cell migration, and angiogenesis.
Dai X, She P, Chi F, Feng Y, Liu H, Jin D, Zhao Y, Guo X, Jiang D, Guan KL, Zhong TP, Zhao B
The Journal of biological chemistry288(47)34041-51(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Thus AMOT is a direct substrate of Lats1/2 mediating functions of the Hippo pathway in endothelial cell migration and angiogenesis.
Title: Phosphorylation of angiomotin by Lats1/2 kinases inhibits F-actin binding, cell migration, and angiogenesis.

lats genes show distinctly different expression profiles during gastrulation. lats1 is almost ubiquitously expressed through development, and lats2 is more prominently expressed in the non-neural ectoderm region of zebrafish gastrula.
Title: Comparative expression of zebrafish lats1 and lats2 and their implication in gastrulation movements.

The following lats1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lats1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa24566	XM_021468417.1 Latest version!	Danio rerio large tumor suppressor kinase 1 (lats1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa24566	XM_009294504.3 Latest version!	Danio rerio large tumor suppressor kinase 1 (lats1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa03864	NM_001020510.1 Latest version!	Danio rerio large tumor suppressor kinase 1 (lats1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa24565	XM_005160312.4 Latest version!	Danio rerio large tumor suppressor kinase 1 (lats1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa24565	XM_005160312.3	Danio rerio large tumor suppressor kinase 1 (lats1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa24566	XM_009294504.2	Danio rerio large tumor suppressor kinase 1 (lats1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lats1 ( XM_021468417.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324092.1 Danio rerio large tumor suppressor kinase 1 (lats1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa24566
Clone ID Related Accession (Same CDS sequence)	XM_021468417.1 , XM_009294504.2 , XM_009294504.3
Accession Version	XM_021468417.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase LATS1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGGG CCGAGAAACC CGAGGGATAC AGACAAATGC GACCCAAGAC GTTCCCTGCC 
AGCAACAACA GCGGCAACAG TCAGCAAATG CTGCAGGAGA TCCGAGAGAG CCTGCGAAAC 
CTCTCCCCGT CCTCCAATGC GTCCAAGCCG GAGCAGAGCT CAGGGAAGGG CCCGCCGGAT 
GAGTCCAGAC AGCAAGGGCG CAGCACCAAC CCCAGAAACT CCCACCACCA CAGAGCCCTG 
CAGGAGATCC GCAAATCCCT GCAGCCGTTT GCCCACGACA GCTCCTCCAG CGGAGACAAC 
AGCTCCGCAG ACAGCAGCAA ACACACACTC CAGACGCAGG AGCCCGGCTT TGATGAGGAG 
CTGATGGTCA ACAGGATAAA CTTCCAGGAG GCTGTGAGGG AGCAGATCCC TTCGGCACGT 
CCTGTGAACA CGGCCATAAA ACAAGCGGGT CCACCCCAAA TTCAACAGCC ATTGTTGCGG 
CGGCAAAGCT GGAAGGGTTC AAAAGAGTCT CTGGCCCCAC AGCGACACAG CGCTTTAATT 
AATGATGTCC TCATTTATCG CCCCAGCAGC CCCGGGCCTC AAAGTGACCT CTCTCGGCCT 
GCCACCTTCC CTCAAAATCT GGCATCTGCC TCCAGCGGCC AGCGAGTGAA CGCTCCCCTG 
TCCCGGCAGG TGCGCAGCAT CACTCCTCCT CCGTCTTCAT GGGACTCCAA TCCCTCAACC 
AAGCGCTATT CTGGGAACTT GGATTACCTG GTGCCCCGGA TCTCCCCAGT GCCCCAAGGC 
CCTCGAGGTG ATGGCTATAT AAATCCTCAG TCCCAAAATC AGCGCGGGCT CAGCCCGGTG 
CCGGTGGGTC GGCAGCCCAT CATCATGCAG AACTCTAACA GCAACAAGTT TAACTTCCCA 
CCATCCTGGC CTCAGAACGG CAGCATGCAA AACGAATACA TGGGCATGAA CAGTGGTGCC 
CGGCAGCCAC CGCCACCTCC CTATTCCATG CACCAAACCA ACAGGCAGAG CCCCACAGCC 
CAGCAGATGC AGTCCAGCGC TCTTACTGCA CCTCCAAACG GAGCCAGCCT CCCTCAGAGC 
ATGCTGGTGC CCAACCGCAA CAGCCACAAC CTGGACATGT ACAACCTTGG AGTAATACGG 
CCGCCAGTGC AGCAGGCTCA GACGCCCCCC GGCCAGGACA CCTCCTCATC ATGGACGCAC 
AGCGTCCCAG GACGCTCCAA CTCTTTCACA AGTGCACCAA ACAATCGACG CCAGTGTCCC 
AACTCACAGC TCTCTGCCAC CACAGTCACC ACAGTAACCC AGGCGCCCAT CTTGCAGCCT 
GTCAAGAGCA TGCGTGTGCA GAAGCCGGAG CTGCACACCG CTGTGGCCCC TGCGCACCCG 
CCCTGGCTGC AGCCACCTCC GCCCACTGCT TACCAAGAGC CGCCCACGCC CCCCGCACCC 
GCTGCTGAAG TCCCGAGCTA CCAGGGCCCA CCGCCTCCCT ATCCCAAACA CCTGCTCCAG 
CAGCAGCAGC CGCCACCAGG GTACGACTCC AGCCCCGCAC TAAAGCCCTG CAGTAAAGAA 
GGGGACGCGG CAGACAACGA AAACGACACA GACAGCACCT GCTCCAGCGA GAGGGCCGAG 
GGCTCGGAGG AAGAGAAGAA GCAGATCACG ACGTCCCCGG TGCCGGTGCG CCGTCACAAA 
CGAGACGAGG AACGCAAGGG CGAAAGCAGA CTTCCCATGT ACTCTCCGCA GGCCTTCAAG 
TTCTTCATGG AGCAGCACGT GGAGAACATC CTGAAGAACC ACCAGCAGAG GATGCGCAGG 
AAGAAGCAGC TGGAGAGCGA GATGCAGCGG TGTGTGGATC TCCTCCTTTT GCCGCTGCCT 
TTCTTTTCTT TGTCTGATCA GCTGGTTTTC ATAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324092.1
CDS	252..2150
Translation	MRRAEKPEGYRQMRPKTFPASNNSGNSQQMLQEIRESLRNLSPSSNASKPEQSSGKGPPDESRQQGRSTNPRNSHHHRALQEIRKSLQPFAHDSSSSGDNSSADSSKHTLQTQEPGFDEELMVNRINFQEAVREQIPSARPVNTAIKQAGPPQIQQPLLRRQSWKGSKESLAPQRHSALINDVLIYRPSSPGPQSDLSRPATFPQNLASASSGQRVNAPLSRQVRSITPPPSSWDSNPSTKRYSGNLDYLVPRISPVPQGPRGDGYINPQSQNQRGLSPVPVGRQPIIMQNSNSNKFNFPPSWPQNGSMQNEYMGMNSGARQPPPPPYSMHQTNRQSPTAQQMQSSALTAPPNGASLPQSMLVPNRNSHNLDMYNLGVIRPPVQQAQTPPGQDTSSSWTHSVPGRSNSFTSAPNNRRQCPNSQLSATTVTTVTQAPILQPVKSMRVQKPELHTAVAPAHPPWLQPPPPTAYQEPPTPPAPAAEVPSYQGPPPPYPKHLLQQQQPPPGYDSSPALKPCSKEGDAADNENDTDSTCSSERAEGSEEEKKQITTSPVPVRRHKRDEERKGESRLPMYSPQAFKFFMEQHVENILKNHQQRMRRKKQLESEMQRCVDLLLLPLPFFSLSDQLVFIV

Target ORF information:

RefSeq Version	XM_021468417.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio large tumor suppressor kinase 1 (lats1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468417.1

ORF Insert Sequence:

ATGAGGAGGG CCGAGAAACC CGAGGGATAC AGACAAATGC GACCCAAGAC GTTCCCTGCC 
AGCAACAACA GCGGCAACAG TCAGCAAATG CTGCAGGAGA TCCGAGAGAG CCTGCGAAAC 
CTCTCCCCGT CCTCCAATGC GTCCAAGCCG GAGCAGAGCT CAGGGAAGGG CCCGCCGGAT 
GAGTCCAGAC AGCAAGGGCG CAGCACCAAC CCCAGAAACT CCCACCACCA CAGAGCCCTG 
CAGGAGATCC GCAAATCCCT GCAGCCGTTT GCCCACGACA GCTCCTCCAG CGGAGACAAC 
AGCTCCGCAG ACAGCAGCAA ACACACACTC CAGACGCAGG AGCCCGGCTT TGATGAGGAG 
CTGATGGTCA ACAGGATAAA CTTCCAGGAG GCTGTGAGGG AGCAGATCCC TTCGGCACGT 
CCTGTGAACA CGGCCATAAA ACAAGCGGGT CCACCCCAAA TTCAACAGCC ATTGTTGCGG 
CGGCAAAGCT GGAAGGGTTC AAAAGAGTCT CTGGCCCCAC AGCGACACAG CGCTTTAATT 
AATGATGTCC TCATTTATCG CCCCAGCAGC CCCGGGCCTC AAAGTGACCT CTCTCGGCCT 
GCCACCTTCC CTCAAAATCT GGCATCTGCC TCCAGCGGCC AGCGAGTGAA CGCTCCCCTG 
TCCCGGCAGG TGCGCAGCAT CACTCCTCCT CCGTCTTCAT GGGACTCCAA TCCCTCAACC 
AAGCGCTATT CTGGGAACTT GGATTACCTG GTGCCCCGGA TCTCCCCAGT GCCCCAAGGC 
CCTCGAGGTG ATGGCTATAT AAATCCTCAG TCCCAAAATC AGCGCGGGCT CAGCCCGGTG 
CCGGTGGGTC GGCAGCCCAT CATCATGCAG AACTCTAACA GCAACAAGTT TAACTTCCCA 
CCATCCTGGC CTCAGAACGG CAGCATGCAA AACGAATACA TGGGCATGAA CAGTGGTGCC 
CGGCAGCCAC CGCCACCTCC CTATTCCATG CACCAAACCA ACAGGCAGAG CCCCACAGCC 
CAGCAGATGC AGTCCAGCGC TCTTACTGCA CCTCCAAACG GAGCCAGCCT CCCTCAGAGC 
ATGCTGGTGC CCAACCGCAA CAGCCACAAC CTGGACATGT ACAACCTTGG AGTAATACGG 
CCGCCAGTGC AGCAGGCTCA GACGCCCCCC GGCCAGGACA CCTCCTCATC ATGGACGCAC 
AGCGTCCCAG GACGCTCCAA CTCTTTCACA AGTGCACCAA ACAATCGACG CCAGTGTCCC 
AACTCACAGC TCTCTGCCAC CACAGTCACC ACAGTAACCC AGGCGCCCAT CTTGCAGCCT 
GTCAAGAGCA TGCGTGTGCA GAAGCCGGAG CTGCACACCG CTGTGGCCCC TGCGCACCCG 
CCCTGGCTGC AGCCACCTCC GCCCACTGCT TACCAAGAGC CGCCCACGCC CCCCGCACCC 
GCTGCTGAAG TCCCGAGCTA CCAGGGCCCA CCGCCTCCCT ATCCCAAACA CCTGCTCCAG 
CAGCAGCAGC CGCCACCAGG GTACGACTCC AGCCCCGCAC TAAAGCCCTG CAGTAAAGAA 
GGGGACGCGG CAGACAACGA AAACGACACA GACAGCACCT GCTCCAGCGA GAGGGCCGAG 
GGCTCGGAGG AAGAGAAGAA GCAGATCACG ACGTCCCCGG TGCCGGTGCG CCGTCACAAA 
CGAGACGAGG AACGCAAGGG CGAAAGCAGA CTTCCCATGT ACTCTCCGCA GGCCTTCAAG 
TTCTTCATGG AGCAGCACGT GGAGAACATC CTGAAGAACC ACCAGCAGAG GATGCGCAGG 
AAGAAGCAGC TGGAGAGCGA GATGCAGCGG TGTGTGGATC TCCTCCTTTT GCCGCTGCCT 
TTCTTTTCTT TGTCTGATCA GCTGGTTTTC ATAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lats1 ( XM_009294504.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292779.1 Danio rerio large tumor suppressor kinase 1 (lats1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa24566
Clone ID Related Accession (Same CDS sequence)	XM_021468417.1 , XM_009294504.2 , XM_009294504.3
Accession Version	XM_009294504.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase LATS1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294504.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGGG CCGAGAAACC CGAGGGATAC AGACAAATGC GACCCAAGAC GTTCCCTGCC 
AGCAACAACA GCGGCAACAG TCAGCAAATG CTGCAGGAGA TCCGAGAGAG CCTGCGAAAC 
CTCTCCCCGT CCTCCAATGC GTCCAAGCCG GAGCAGAGCT CAGGGAAGGG CCCGCCGGAT 
GAGTCCAGAC AGCAAGGGCG CAGCACCAAC CCCAGAAACT CCCACCACCA CAGAGCCCTG 
CAGGAGATCC GCAAATCCCT GCAGCCGTTT GCCCACGACA GCTCCTCCAG CGGAGACAAC 
AGCTCCGCAG ACAGCAGCAA ACACACACTC CAGACGCAGG AGCCCGGCTT TGATGAGGAG 
CTGATGGTCA ACAGGATAAA CTTCCAGGAG GCTGTGAGGG AGCAGATCCC TTCGGCACGT 
CCTGTGAACA CGGCCATAAA ACAAGCGGGT CCACCCCAAA TTCAACAGCC ATTGTTGCGG 
CGGCAAAGCT GGAAGGGTTC AAAAGAGTCT CTGGCCCCAC AGCGACACAG CGCTTTAATT 
AATGATGTCC TCATTTATCG CCCCAGCAGC CCCGGGCCTC AAAGTGACCT CTCTCGGCCT 
GCCACCTTCC CTCAAAATCT GGCATCTGCC TCCAGCGGCC AGCGAGTGAA CGCTCCCCTG 
TCCCGGCAGG TGCGCAGCAT CACTCCTCCT CCGTCTTCAT GGGACTCCAA TCCCTCAACC 
AAGCGCTATT CTGGGAACTT GGATTACCTG GTGCCCCGGA TCTCCCCAGT GCCCCAAGGC 
CCTCGAGGTG ATGGCTATAT AAATCCTCAG TCCCAAAATC AGCGCGGGCT CAGCCCGGTG 
CCGGTGGGTC GGCAGCCCAT CATCATGCAG AACTCTAACA GCAACAAGTT TAACTTCCCA 
CCATCCTGGC CTCAGAACGG CAGCATGCAA AACGAATACA TGGGCATGAA CAGTGGTGCC 
CGGCAGCCAC CGCCACCTCC CTATTCCATG CACCAAACCA ACAGGCAGAG CCCCACAGCC 
CAGCAGATGC AGTCCAGCGC TCTTACTGCA CCTCCAAACG GAGCCAGCCT CCCTCAGAGC 
ATGCTGGTGC CCAACCGCAA CAGCCACAAC CTGGACATGT ACAACCTTGG AGTAATACGG 
CCGCCAGTGC AGCAGGCTCA GACGCCCCCC GGCCAGGACA CCTCCTCATC ATGGACGCAC 
AGCGTCCCAG GACGCTCCAA CTCTTTCACA AGTGCACCAA ACAATCGACG CCAGTGTCCC 
AACTCACAGC TCTCTGCCAC CACAGTCACC ACAGTAACCC AGGCGCCCAT CTTGCAGCCT 
GTCAAGAGCA TGCGTGTGCA GAAGCCGGAG CTGCACACCG CTGTGGCCCC TGCGCACCCG 
CCCTGGCTGC AGCCACCTCC GCCCACTGCT TACCAAGAGC CGCCCACGCC CCCCGCACCC 
GCTGCTGAAG TCCCGAGCTA CCAGGGCCCA CCGCCTCCCT ATCCCAAACA CCTGCTCCAG 
CAGCAGCAGC CGCCACCAGG GTACGACTCC AGCCCCGCAC TAAAGCCCTG CAGTAAAGAA 
GGGGACGCGG CAGACAACGA AAACGACACA GACAGCACCT GCTCCAGCGA GAGGGCCGAG 
GGCTCGGAGG AAGAGAAGAA GCAGATCACG ACGTCCCCGG TGCCGGTGCG CCGTCACAAA 
CGAGACGAGG AACGCAAGGG CGAAAGCAGA CTTCCCATGT ACTCTCCGCA GGCCTTCAAG 
TTCTTCATGG AGCAGCACGT GGAGAACATC CTGAAGAACC ACCAGCAGAG GATGCGCAGG 
AAGAAGCAGC TGGAGAGCGA GATGCAGCGG TGTGTGGATC TCCTCCTTTT GCCGCTGCCT 
TTCTTTTCTT TGTCTGATCA GCTGGTTTTC ATAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292779.1
CDS	255..2153
Translation	MRRAEKPEGYRQMRPKTFPASNNSGNSQQMLQEIRESLRNLSPSSNASKPEQSSGKGPPDESRQQGRSTNPRNSHHHRALQEIRKSLQPFAHDSSSSGDNSSADSSKHTLQTQEPGFDEELMVNRINFQEAVREQIPSARPVNTAIKQAGPPQIQQPLLRRQSWKGSKESLAPQRHSALINDVLIYRPSSPGPQSDLSRPATFPQNLASASSGQRVNAPLSRQVRSITPPPSSWDSNPSTKRYSGNLDYLVPRISPVPQGPRGDGYINPQSQNQRGLSPVPVGRQPIIMQNSNSNKFNFPPSWPQNGSMQNEYMGMNSGARQPPPPPYSMHQTNRQSPTAQQMQSSALTAPPNGASLPQSMLVPNRNSHNLDMYNLGVIRPPVQQAQTPPGQDTSSSWTHSVPGRSNSFTSAPNNRRQCPNSQLSATTVTTVTQAPILQPVKSMRVQKPELHTAVAPAHPPWLQPPPPTAYQEPPTPPAPAAEVPSYQGPPPPYPKHLLQQQQPPPGYDSSPALKPCSKEGDAADNENDTDSTCSSERAEGSEEEKKQITTSPVPVRRHKRDEERKGESRLPMYSPQAFKFFMEQHVENILKNHQQRMRRKKQLESEMQRCVDLLLLPLPFFSLSDQLVFIV

Target ORF information:

RefSeq Version	XM_009294504.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio large tumor suppressor kinase 1 (lats1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294504.2

ORF Insert Sequence:

ATGAGGAGGG CCGAGAAACC CGAGGGATAC AGACAAATGC GACCCAAGAC GTTCCCTGCC 
AGCAACAACA GCGGCAACAG TCAGCAAATG CTGCAGGAGA TCCGAGAGAG CCTGCGAAAC 
CTCTCCCCGT CCTCCAATGC GTCCAAGCCG GAGCAGAGCT CAGGGAAGGG CCCGCCGGAT 
GAGTCCAGAC AGCAAGGGCG CAGCACCAAC CCCAGAAACT CCCACCACCA CAGAGCCCTG 
CAGGAGATCC GCAAATCCCT GCAGCCGTTT GCCCACGACA GCTCCTCCAG CGGAGACAAC 
AGCTCCGCAG ACAGCAGCAA ACACACACTC CAGACGCAGG AGCCCGGCTT TGATGAGGAG 
CTGATGGTCA ACAGGATAAA CTTCCAGGAG GCTGTGAGGG AGCAGATCCC TTCGGCACGT 
CCTGTGAACA CGGCCATAAA ACAAGCGGGT CCACCCCAAA TTCAACAGCC ATTGTTGCGG 
CGGCAAAGCT GGAAGGGTTC AAAAGAGTCT CTGGCCCCAC AGCGACACAG CGCTTTAATT 
AATGATGTCC TCATTTATCG CCCCAGCAGC CCCGGGCCTC AAAGTGACCT CTCTCGGCCT 
GCCACCTTCC CTCAAAATCT GGCATCTGCC TCCAGCGGCC AGCGAGTGAA CGCTCCCCTG 
TCCCGGCAGG TGCGCAGCAT CACTCCTCCT CCGTCTTCAT GGGACTCCAA TCCCTCAACC 
AAGCGCTATT CTGGGAACTT GGATTACCTG GTGCCCCGGA TCTCCCCAGT GCCCCAAGGC 
CCTCGAGGTG ATGGCTATAT AAATCCTCAG TCCCAAAATC AGCGCGGGCT CAGCCCGGTG 
CCGGTGGGTC GGCAGCCCAT CATCATGCAG AACTCTAACA GCAACAAGTT TAACTTCCCA 
CCATCCTGGC CTCAGAACGG CAGCATGCAA AACGAATACA TGGGCATGAA CAGTGGTGCC 
CGGCAGCCAC CGCCACCTCC CTATTCCATG CACCAAACCA ACAGGCAGAG CCCCACAGCC 
CAGCAGATGC AGTCCAGCGC TCTTACTGCA CCTCCAAACG GAGCCAGCCT CCCTCAGAGC 
ATGCTGGTGC CCAACCGCAA CAGCCACAAC CTGGACATGT ACAACCTTGG AGTAATACGG 
CCGCCAGTGC AGCAGGCTCA GACGCCCCCC GGCCAGGACA CCTCCTCATC ATGGACGCAC 
AGCGTCCCAG GACGCTCCAA CTCTTTCACA AGTGCACCAA ACAATCGACG CCAGTGTCCC 
AACTCACAGC TCTCTGCCAC CACAGTCACC ACAGTAACCC AGGCGCCCAT CTTGCAGCCT 
GTCAAGAGCA TGCGTGTGCA GAAGCCGGAG CTGCACACCG CTGTGGCCCC TGCGCACCCG 
CCCTGGCTGC AGCCACCTCC GCCCACTGCT TACCAAGAGC CGCCCACGCC CCCCGCACCC 
GCTGCTGAAG TCCCGAGCTA CCAGGGCCCA CCGCCTCCCT ATCCCAAACA CCTGCTCCAG 
CAGCAGCAGC CGCCACCAGG GTACGACTCC AGCCCCGCAC TAAAGCCCTG CAGTAAAGAA 
GGGGACGCGG CAGACAACGA AAACGACACA GACAGCACCT GCTCCAGCGA GAGGGCCGAG 
GGCTCGGAGG AAGAGAAGAA GCAGATCACG ACGTCCCCGG TGCCGGTGCG CCGTCACAAA 
CGAGACGAGG AACGCAAGGG CGAAAGCAGA CTTCCCATGT ACTCTCCGCA GGCCTTCAAG 
TTCTTCATGG AGCAGCACGT GGAGAACATC CTGAAGAACC ACCAGCAGAG GATGCGCAGG 
AAGAAGCAGC TGGAGAGCGA GATGCAGCGG TGTGTGGATC TCCTCCTTTT GCCGCTGCCT 
TTCTTTTCTT TGTCTGATCA GCTGGTTTTC ATAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lats1 ( XM_009294504.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292779.1 Danio rerio large tumor suppressor kinase 1 (lats1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa24566
Clone ID Related Accession (Same CDS sequence)	XM_021468417.1 , XM_009294504.2 , XM_009294504.3
Accession Version	XM_009294504.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase LATS1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294504.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGGG CCGAGAAACC CGAGGGATAC AGACAAATGC GACCCAAGAC GTTCCCTGCC 
AGCAACAACA GCGGCAACAG TCAGCAAATG CTGCAGGAGA TCCGAGAGAG CCTGCGAAAC 
CTCTCCCCGT CCTCCAATGC GTCCAAGCCG GAGCAGAGCT CAGGGAAGGG CCCGCCGGAT 
GAGTCCAGAC AGCAAGGGCG CAGCACCAAC CCCAGAAACT CCCACCACCA CAGAGCCCTG 
CAGGAGATCC GCAAATCCCT GCAGCCGTTT GCCCACGACA GCTCCTCCAG CGGAGACAAC 
AGCTCCGCAG ACAGCAGCAA ACACACACTC CAGACGCAGG AGCCCGGCTT TGATGAGGAG 
CTGATGGTCA ACAGGATAAA CTTCCAGGAG GCTGTGAGGG AGCAGATCCC TTCGGCACGT 
CCTGTGAACA CGGCCATAAA ACAAGCGGGT CCACCCCAAA TTCAACAGCC ATTGTTGCGG 
CGGCAAAGCT GGAAGGGTTC AAAAGAGTCT CTGGCCCCAC AGCGACACAG CGCTTTAATT 
AATGATGTCC TCATTTATCG CCCCAGCAGC CCCGGGCCTC AAAGTGACCT CTCTCGGCCT 
GCCACCTTCC CTCAAAATCT GGCATCTGCC TCCAGCGGCC AGCGAGTGAA CGCTCCCCTG 
TCCCGGCAGG TGCGCAGCAT CACTCCTCCT CCGTCTTCAT GGGACTCCAA TCCCTCAACC 
AAGCGCTATT CTGGGAACTT GGATTACCTG GTGCCCCGGA TCTCCCCAGT GCCCCAAGGC 
CCTCGAGGTG ATGGCTATAT AAATCCTCAG TCCCAAAATC AGCGCGGGCT CAGCCCGGTG 
CCGGTGGGTC GGCAGCCCAT CATCATGCAG AACTCTAACA GCAACAAGTT TAACTTCCCA 
CCATCCTGGC CTCAGAACGG CAGCATGCAA AACGAATACA TGGGCATGAA CAGTGGTGCC 
CGGCAGCCAC CGCCACCTCC CTATTCCATG CACCAAACCA ACAGGCAGAG CCCCACAGCC 
CAGCAGATGC AGTCCAGCGC TCTTACTGCA CCTCCAAACG GAGCCAGCCT CCCTCAGAGC 
ATGCTGGTGC CCAACCGCAA CAGCCACAAC CTGGACATGT ACAACCTTGG AGTAATACGG 
CCGCCAGTGC AGCAGGCTCA GACGCCCCCC GGCCAGGACA CCTCCTCATC ATGGACGCAC 
AGCGTCCCAG GACGCTCCAA CTCTTTCACA AGTGCACCAA ACAATCGACG CCAGTGTCCC 
AACTCACAGC TCTCTGCCAC CACAGTCACC ACAGTAACCC AGGCGCCCAT CTTGCAGCCT 
GTCAAGAGCA TGCGTGTGCA GAAGCCGGAG CTGCACACCG CTGTGGCCCC TGCGCACCCG 
CCCTGGCTGC AGCCACCTCC GCCCACTGCT TACCAAGAGC CGCCCACGCC CCCCGCACCC 
GCTGCTGAAG TCCCGAGCTA CCAGGGCCCA CCGCCTCCCT ATCCCAAACA CCTGCTCCAG 
CAGCAGCAGC CGCCACCAGG GTACGACTCC AGCCCCGCAC TAAAGCCCTG CAGTAAAGAA 
GGGGACGCGG CAGACAACGA AAACGACACA GACAGCACCT GCTCCAGCGA GAGGGCCGAG 
GGCTCGGAGG AAGAGAAGAA GCAGATCACG ACGTCCCCGG TGCCGGTGCG CCGTCACAAA 
CGAGACGAGG AACGCAAGGG CGAAAGCAGA CTTCCCATGT ACTCTCCGCA GGCCTTCAAG 
TTCTTCATGG AGCAGCACGT GGAGAACATC CTGAAGAACC ACCAGCAGAG GATGCGCAGG 
AAGAAGCAGC TGGAGAGCGA GATGCAGCGG TGTGTGGATC TCCTCCTTTT GCCGCTGCCT 
TTCTTTTCTT TGTCTGATCA GCTGGTTTTC ATAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292779.1
CDS	252..2150
Translation	MRRAEKPEGYRQMRPKTFPASNNSGNSQQMLQEIRESLRNLSPSSNASKPEQSSGKGPPDESRQQGRSTNPRNSHHHRALQEIRKSLQPFAHDSSSSGDNSSADSSKHTLQTQEPGFDEELMVNRINFQEAVREQIPSARPVNTAIKQAGPPQIQQPLLRRQSWKGSKESLAPQRHSALINDVLIYRPSSPGPQSDLSRPATFPQNLASASSGQRVNAPLSRQVRSITPPPSSWDSNPSTKRYSGNLDYLVPRISPVPQGPRGDGYINPQSQNQRGLSPVPVGRQPIIMQNSNSNKFNFPPSWPQNGSMQNEYMGMNSGARQPPPPPYSMHQTNRQSPTAQQMQSSALTAPPNGASLPQSMLVPNRNSHNLDMYNLGVIRPPVQQAQTPPGQDTSSSWTHSVPGRSNSFTSAPNNRRQCPNSQLSATTVTTVTQAPILQPVKSMRVQKPELHTAVAPAHPPWLQPPPPTAYQEPPTPPAPAAEVPSYQGPPPPYPKHLLQQQQPPPGYDSSPALKPCSKEGDAADNENDTDSTCSSERAEGSEEEKKQITTSPVPVRRHKRDEERKGESRLPMYSPQAFKFFMEQHVENILKNHQQRMRRKKQLESEMQRCVDLLLLPLPFFSLSDQLVFIV

Target ORF information:

RefSeq Version	XM_009294504.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio large tumor suppressor kinase 1 (lats1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294504.3

ORF Insert Sequence:

ATGAGGAGGG CCGAGAAACC CGAGGGATAC AGACAAATGC GACCCAAGAC GTTCCCTGCC 
AGCAACAACA GCGGCAACAG TCAGCAAATG CTGCAGGAGA TCCGAGAGAG CCTGCGAAAC 
CTCTCCCCGT CCTCCAATGC GTCCAAGCCG GAGCAGAGCT CAGGGAAGGG CCCGCCGGAT 
GAGTCCAGAC AGCAAGGGCG CAGCACCAAC CCCAGAAACT CCCACCACCA CAGAGCCCTG 
CAGGAGATCC GCAAATCCCT GCAGCCGTTT GCCCACGACA GCTCCTCCAG CGGAGACAAC 
AGCTCCGCAG ACAGCAGCAA ACACACACTC CAGACGCAGG AGCCCGGCTT TGATGAGGAG 
CTGATGGTCA ACAGGATAAA CTTCCAGGAG GCTGTGAGGG AGCAGATCCC TTCGGCACGT 
CCTGTGAACA CGGCCATAAA ACAAGCGGGT CCACCCCAAA TTCAACAGCC ATTGTTGCGG 
CGGCAAAGCT GGAAGGGTTC AAAAGAGTCT CTGGCCCCAC AGCGACACAG CGCTTTAATT 
AATGATGTCC TCATTTATCG CCCCAGCAGC CCCGGGCCTC AAAGTGACCT CTCTCGGCCT 
GCCACCTTCC CTCAAAATCT GGCATCTGCC TCCAGCGGCC AGCGAGTGAA CGCTCCCCTG 
TCCCGGCAGG TGCGCAGCAT CACTCCTCCT CCGTCTTCAT GGGACTCCAA TCCCTCAACC 
AAGCGCTATT CTGGGAACTT GGATTACCTG GTGCCCCGGA TCTCCCCAGT GCCCCAAGGC 
CCTCGAGGTG ATGGCTATAT AAATCCTCAG TCCCAAAATC AGCGCGGGCT CAGCCCGGTG 
CCGGTGGGTC GGCAGCCCAT CATCATGCAG AACTCTAACA GCAACAAGTT TAACTTCCCA 
CCATCCTGGC CTCAGAACGG CAGCATGCAA AACGAATACA TGGGCATGAA CAGTGGTGCC 
CGGCAGCCAC CGCCACCTCC CTATTCCATG CACCAAACCA ACAGGCAGAG CCCCACAGCC 
CAGCAGATGC AGTCCAGCGC TCTTACTGCA CCTCCAAACG GAGCCAGCCT CCCTCAGAGC 
ATGCTGGTGC CCAACCGCAA CAGCCACAAC CTGGACATGT ACAACCTTGG AGTAATACGG 
CCGCCAGTGC AGCAGGCTCA GACGCCCCCC GGCCAGGACA CCTCCTCATC ATGGACGCAC 
AGCGTCCCAG GACGCTCCAA CTCTTTCACA AGTGCACCAA ACAATCGACG CCAGTGTCCC 
AACTCACAGC TCTCTGCCAC CACAGTCACC ACAGTAACCC AGGCGCCCAT CTTGCAGCCT 
GTCAAGAGCA TGCGTGTGCA GAAGCCGGAG CTGCACACCG CTGTGGCCCC TGCGCACCCG 
CCCTGGCTGC AGCCACCTCC GCCCACTGCT TACCAAGAGC CGCCCACGCC CCCCGCACCC 
GCTGCTGAAG TCCCGAGCTA CCAGGGCCCA CCGCCTCCCT ATCCCAAACA CCTGCTCCAG 
CAGCAGCAGC CGCCACCAGG GTACGACTCC AGCCCCGCAC TAAAGCCCTG CAGTAAAGAA 
GGGGACGCGG CAGACAACGA AAACGACACA GACAGCACCT GCTCCAGCGA GAGGGCCGAG 
GGCTCGGAGG AAGAGAAGAA GCAGATCACG ACGTCCCCGG TGCCGGTGCG CCGTCACAAA 
CGAGACGAGG AACGCAAGGG CGAAAGCAGA CTTCCCATGT ACTCTCCGCA GGCCTTCAAG 
TTCTTCATGG AGCAGCACGT GGAGAACATC CTGAAGAACC ACCAGCAGAG GATGCGCAGG 
AAGAAGCAGC TGGAGAGCGA GATGCAGCGG TGTGTGGATC TCCTCCTTTT GCCGCTGCCT 
TTCTTTTCTT TGTCTGATCA GCTGGTTTTC ATAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lats1 ( NM_001020510.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001018346.1 Danio rerio large tumor suppressor kinase 1 (lats1), mRNA.

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CloneID	ODa03864
Clone ID Related Accession (Same CDS sequence)	NM_001020510.1
Accession Version	NM_001020510.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-21
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase LATS1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY957463.1. ##Evidence-Data-START## Transcript exon combination :: AY957463.1, BC171344.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476730, SAMEA4476732 [ECO:0000348] ##Evidence-Data-END##

ATGAGGAGGG CCGAGAAACC CGAGGGATAC AGACAAATGC GACCCAAGAC GTTCCCTGCC 
AGCAACAACA GCGGCAACAG TCAGCAAATG CTGCAGGAGA TCCGAGAGAG CCTGCGAAAC 
CTCTCCCCGT CCTCCAATGC GTCCAAGCCG GAGCAGAGCT CAGGGAAGGG CCCGCCGGAT 
GAGTCCCGAC AGCAGGGGCG CAGCACCAAC CCCAGAAACT CCCACCACCA CAGAGCCCTG 
CAGGAGATCC GCAAATCCCT GCAACCGTTC GCCCACGACA GCTCCTCCAG CGGAGACAAC 
AGCTCCGCAG ACAGCAGCAA ACACACACTC CAGACGCAGG AGCCCGGCTT TGATGAGGAG 
CTGATGGTCA ACAGGATAAA CTTCCAGGAG GCTGTGAGGG AGCAGATCCC TTCGGCACGT 
CCTGTGAACA CGGCCATAAA ACAAGCGGGT CCACCCCAAA TTCAACAGCC ATTGTTGCGG 
CGGCAAAGCT GGAAGGGTTC AAAAGAGTCT CTGGCCCCAC AGCGACACAG CGCTTTAATT 
AATGATGTCC TCATTTATCG CCCCAGCAGC CCCGGGCCTC AAAGTGACCT CTCTCGGCCT 
GCCACCTTCC CTCAAAATCT GGCTTCTGCC TCCAGCGGCC AGCGAGTGAA CGCTCCCCTG 
TCCCGGCAGG TGCGCAGCAT CACTCCTCCT CCGTCTTCAT GGGACTCCAA TCCCTCAACC 
AAGCGCTATT CTGGGAACTT GGATTACCTG GTGCCCCGGA TCTCCCCAGT GCCCCAAGGC 
CCTCGAGGTG ATGGCTATAT AAACCCTCAG TCCCAAAATC AGCGCGGGCT CCGCCCGGTG 
CCGGTGGGTC GGCAGCCCAT CATCATGCAG AACTCTAACA GCAACAAGTT TAACTTCCCA 
CCATCCTGGC CTCAGAACGG CAGCATGCAA AACGAATACA TGGGCATGAA CAGTGGTGCC 
CGGCAGCCAC CGCCACCTCC CTATTCCATG CACCAAACCA ACAGGCAGAG CCCCACAGCC 
CAGCAGATGC AGTCCAGCGC TCTTACTGCA CCTCCAAATG GAGCCAGCCT CCCTCAGAGC 
ATGCTGGTGC CCAACCGCAA CAGCCACAAC CTGGACATGT ACAACCTTGG AGTAATACGG 
CCGCCAGTGC AGCAGGCTCA GACGCCCCCC GGCCAGGACA CCTCCTCATC ATGGACGCAC 
AGCGTCCCAG GACGCTCCAA CTCTTTCACA AGTGCACCAA ACAATCGACG CCAGTGTCCC 
AACTCACAGC TCTCTGCCAC CACAGTCACC ACAGTAACCC AGGCGCCCAT CTTGCAGCCT 
GTCAAGAGCA TGCGTGTGCA GAAGCCGGAG CTGCACACCG CTGTGGCCCC TGCGCACCCG 
CCCTGGCTGC AGCCACCTCC GCCCACTGCT TACCAAGAGC CGCCTACACC CCCCGCACCC 
GCTGCTGAAG TCCCGAGCTA CCAGGGCCCA CCGCCTCCCT ATCCCAAACA CCTGCTCCAG 
CAGCAGCAGC CGCCACCAGG GTACGACTCC AGCCCCGCAC TAAAGCCCTG CAGTAAAGAA 
GGGGACGCGG CAGACAACGA AAACGACACA GACAGCACCT GCTCCAGCGA GAGGGCCGAG 
GGCTCGGAGG AAGAGAAGAA GCAGATCACG ACGTCCCCGG TGCCGGTGCG CCGTCACAAA 
CGAGACGAGG AACGCAAGGG CGAAAGCAGA CTTCCCATGT ACTCTCCGCA GGCCTTCAAG 
TTCTTCATGG AGCAGCACGT GGAGAACATC CTGAAGAACC ACCAGCAGAG GATGCGCAGG 
AAGAAGCAGC TGGAGAGCGA GATGCAGCGG GTGGGACTCT CGGGCGACGC ACAGGAGCAG 
ATGCGCATGA TGCTGAGCCA GAAGGAGTCC AACTACATCC GCCTCAAGCG CGCCAAAATG 
GACAAATGCA TGTTCGAGAA GATCAAGACT CTGGGAATCG GGGCGTTTGG AGAAGTGTGT 
TTGGCTCGGA AAGTGGACAC TGGAGCTCTG TACGCCATGA AGACCCTCCG TAAAAAAGAC 
GTGTTGTTAA GGAACCAGGT AGCCCACGTG AAAGCCGAGC GGGATATTCT CGCTGAAGCG 
GATAACGAGT GGGTCGTCCG CTTGTACTAC TCTTTCCAGG ATAAAGATAA CTTGTACTTT 
GTCATGGACT ACATCCCTGG AGGAGACATG ATGAGTCTTC TGATCCGAAT GGGCATTTTT 
CAGGAGGACT TGGCTCAGTT TTACATCGCA GAGCTCATCT GTGCTGTGGA AAGCGTCCAC 
AAAATGGGTT TCATCCATCG AGACATCAAA CCTGACAACA TCCTGATCGA CCGTGATGGA 
CACATCAAAC TGACAGACTT CGGCCTCTGC ACTGGATTCA GATGGACTCA CGACTCGAAA 
TACTACCAAA GCGGTGATCA TGTGCGTCAG GACAGCATGG ACTTCAGTAT GGAGTGGGAG 
GATTCCGCAA ACTGCCGCTG CGGGGATCGC CTGAAGCCCC TGGAGCGCAG AGCAGCTAGA 
CAACACCAGC GCTGCCTGGC ACACTCTCTA GTGGGAACGC CCAATTACAT CGCACCCGAG 
GTTCTGCTGC GCACCGGCTA CACGCAGCTG TGTGATTGGT GGAGTGTTGG CGTTATCCTC 
TATGAGATGG TCGTCGGTCA GCCTCCGTTT CTGGCCACAA CTCCTCTGGA GACGCAGATG 
AAGGTGATCC GCTGGCAGAC GTCACTCCAC ATCCCACTGC AGGCCAAGCT CAGCCCGGAG 
GCCACAGACC TGATCCTGAA GCTGTGCCGT GGCCCGGATG ACCGGCTGGG CAGAAACGGA 
GCCGATGAGA TCAAAGCACA GCCCTTCTTC AGGACTATCG ACCTCTCCAA GGACCTGCGG 
CAGCAACATC AAGCCCCCTA CATCCCCAAA ATCACACACT CCACCGACAC CTCCAACTTC 
GACCCAGTGG ACCCGGACAA ACTGTGGAGC GAGGACGCGG ACGGAAATCA CAACGACACG 
CTCACCCGCT GGTTCAAGAA CGGCAAGCAC CCCGAGCACG CCTTCTACGA GTTCACCTTC 
CGCCGCTTTT TCGACGACAG CGGCCACCCG TACAGCTGCC CCAAGCCCAT CGAGAGCGAG 
GAGGAGGACG AGGACGAAGA CTACGTGGAA GAGGACGAGC CGTCGCAGCG GCCGATGGAG 
CAGAGCCGAG ATCTTGTGTA CGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018346.1
CDS	1..3207
Translation	MRRAEKPEGYRQMRPKTFPASNNSGNSQQMLQEIRESLRNLSPSSNASKPEQSSGKGPPDESRQQGRSTNPRNSHHHRALQEIRKSLQPFAHDSSSSGDNSSADSSKHTLQTQEPGFDEELMVNRINFQEAVREQIPSARPVNTAIKQAGPPQIQQPLLRRQSWKGSKESLAPQRHSALINDVLIYRPSSPGPQSDLSRPATFPQNLASASSGQRVNAPLSRQVRSITPPPSSWDSNPSTKRYSGNLDYLVPRISPVPQGPRGDGYINPQSQNQRGLRPVPVGRQPIIMQNSNSNKFNFPPSWPQNGSMQNEYMGMNSGARQPPPPPYSMHQTNRQSPTAQQMQSSALTAPPNGASLPQSMLVPNRNSHNLDMYNLGVIRPPVQQAQTPPGQDTSSSWTHSVPGRSNSFTSAPNNRRQCPNSQLSATTVTTVTQAPILQPVKSMRVQKPELHTAVAPAHPPWLQPPPPTAYQEPPTPPAPAAEVPSYQGPPPPYPKHLLQQQQPPPGYDSSPALKPCSKEGDAADNENDTDSTCSSERAEGSEEEKKQITTSPVPVRRHKRDEERKGESRLPMYSPQAFKFFMEQHVENILKNHQQRMRRKKQLESEMQRVGLSGDAQEQMRMMLSQKESNYIRLKRAKMDKCMFEKIKTLGIGAFGEVCLARKVDTGALYAMKTLRKKDVLLRNQVAHVKAERDILAEADNEWVVRLYYSFQDKDNLYFVMDYIPGGDMMSLLIRMGIFQEDLAQFYIAELICAVESVHKMGFIHRDIKPDNILIDRDGHIKLTDFGLCTGFRWTHDSKYYQSGDHVRQDSMDFSMEWEDSANCRCGDRLKPLERRAARQHQRCLAHSLVGTPNYIAPEVLLRTGYTQLCDWWSVGVILYEMVVGQPPFLATTPLETQMKVIRWQTSLHIPLQAKLSPEATDLILKLCRGPDDRLGRNGADEIKAQPFFRTIDLSKDLRQQHQAPYIPKITHSTDTSNFDPVDPDKLWSEDADGNHNDTLTRWFKNGKHPEHAFYEFTFRRFFDDSGHPYSCPKPIESEEEDEDEDYVEEDEPSQRPMEQSRDLVYV

Target ORF information:

RefSeq Version	NM_001020510.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio large tumor suppressor kinase 1 (lats1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020510.1

ORF Insert Sequence:

ATGAGGAGGG CCGAGAAACC CGAGGGATAC AGACAAATGC GACCCAAGAC GTTCCCTGCC 
AGCAACAACA GCGGCAACAG TCAGCAAATG CTGCAGGAGA TCCGAGAGAG CCTGCGAAAC 
CTCTCCCCGT CCTCCAATGC GTCCAAGCCG GAGCAGAGCT CAGGGAAGGG CCCGCCGGAT 
GAGTCCCGAC AGCAGGGGCG CAGCACCAAC CCCAGAAACT CCCACCACCA CAGAGCCCTG 
CAGGAGATCC GCAAATCCCT GCAACCGTTC GCCCACGACA GCTCCTCCAG CGGAGACAAC 
AGCTCCGCAG ACAGCAGCAA ACACACACTC CAGACGCAGG AGCCCGGCTT TGATGAGGAG 
CTGATGGTCA ACAGGATAAA CTTCCAGGAG GCTGTGAGGG AGCAGATCCC TTCGGCACGT 
CCTGTGAACA CGGCCATAAA ACAAGCGGGT CCACCCCAAA TTCAACAGCC ATTGTTGCGG 
CGGCAAAGCT GGAAGGGTTC AAAAGAGTCT CTGGCCCCAC AGCGACACAG CGCTTTAATT 
AATGATGTCC TCATTTATCG CCCCAGCAGC CCCGGGCCTC AAAGTGACCT CTCTCGGCCT 
GCCACCTTCC CTCAAAATCT GGCTTCTGCC TCCAGCGGCC AGCGAGTGAA CGCTCCCCTG 
TCCCGGCAGG TGCGCAGCAT CACTCCTCCT CCGTCTTCAT GGGACTCCAA TCCCTCAACC 
AAGCGCTATT CTGGGAACTT GGATTACCTG GTGCCCCGGA TCTCCCCAGT GCCCCAAGGC 
CCTCGAGGTG ATGGCTATAT AAACCCTCAG TCCCAAAATC AGCGCGGGCT CCGCCCGGTG 
CCGGTGGGTC GGCAGCCCAT CATCATGCAG AACTCTAACA GCAACAAGTT TAACTTCCCA 
CCATCCTGGC CTCAGAACGG CAGCATGCAA AACGAATACA TGGGCATGAA CAGTGGTGCC 
CGGCAGCCAC CGCCACCTCC CTATTCCATG CACCAAACCA ACAGGCAGAG CCCCACAGCC 
CAGCAGATGC AGTCCAGCGC TCTTACTGCA CCTCCAAATG GAGCCAGCCT CCCTCAGAGC 
ATGCTGGTGC CCAACCGCAA CAGCCACAAC CTGGACATGT ACAACCTTGG AGTAATACGG 
CCGCCAGTGC AGCAGGCTCA GACGCCCCCC GGCCAGGACA CCTCCTCATC ATGGACGCAC 
AGCGTCCCAG GACGCTCCAA CTCTTTCACA AGTGCACCAA ACAATCGACG CCAGTGTCCC 
AACTCACAGC TCTCTGCCAC CACAGTCACC ACAGTAACCC AGGCGCCCAT CTTGCAGCCT 
GTCAAGAGCA TGCGTGTGCA GAAGCCGGAG CTGCACACCG CTGTGGCCCC TGCGCACCCG 
CCCTGGCTGC AGCCACCTCC GCCCACTGCT TACCAAGAGC CGCCTACACC CCCCGCACCC 
GCTGCTGAAG TCCCGAGCTA CCAGGGCCCA CCGCCTCCCT ATCCCAAACA CCTGCTCCAG 
CAGCAGCAGC CGCCACCAGG GTACGACTCC AGCCCCGCAC TAAAGCCCTG CAGTAAAGAA 
GGGGACGCGG CAGACAACGA AAACGACACA GACAGCACCT GCTCCAGCGA GAGGGCCGAG 
GGCTCGGAGG AAGAGAAGAA GCAGATCACG ACGTCCCCGG TGCCGGTGCG CCGTCACAAA 
CGAGACGAGG AACGCAAGGG CGAAAGCAGA CTTCCCATGT ACTCTCCGCA GGCCTTCAAG 
TTCTTCATGG AGCAGCACGT GGAGAACATC CTGAAGAACC ACCAGCAGAG GATGCGCAGG 
AAGAAGCAGC TGGAGAGCGA GATGCAGCGG GTGGGACTCT CGGGCGACGC ACAGGAGCAG 
ATGCGCATGA TGCTGAGCCA GAAGGAGTCC AACTACATCC GCCTCAAGCG CGCCAAAATG 
GACAAATGCA TGTTCGAGAA GATCAAGACT CTGGGAATCG GGGCGTTTGG AGAAGTGTGT 
TTGGCTCGGA AAGTGGACAC TGGAGCTCTG TACGCCATGA AGACCCTCCG TAAAAAAGAC 
GTGTTGTTAA GGAACCAGGT AGCCCACGTG AAAGCCGAGC GGGATATTCT CGCTGAAGCG 
GATAACGAGT GGGTCGTCCG CTTGTACTAC TCTTTCCAGG ATAAAGATAA CTTGTACTTT 
GTCATGGACT ACATCCCTGG AGGAGACATG ATGAGTCTTC TGATCCGAAT GGGCATTTTT 
CAGGAGGACT TGGCTCAGTT TTACATCGCA GAGCTCATCT GTGCTGTGGA AAGCGTCCAC 
AAAATGGGTT TCATCCATCG AGACATCAAA CCTGACAACA TCCTGATCGA CCGTGATGGA 
CACATCAAAC TGACAGACTT CGGCCTCTGC ACTGGATTCA GATGGACTCA CGACTCGAAA 
TACTACCAAA GCGGTGATCA TGTGCGTCAG GACAGCATGG ACTTCAGTAT GGAGTGGGAG 
GATTCCGCAA ACTGCCGCTG CGGGGATCGC CTGAAGCCCC TGGAGCGCAG AGCAGCTAGA 
CAACACCAGC GCTGCCTGGC ACACTCTCTA GTGGGAACGC CCAATTACAT CGCACCCGAG 
GTTCTGCTGC GCACCGGCTA CACGCAGCTG TGTGATTGGT GGAGTGTTGG CGTTATCCTC 
TATGAGATGG TCGTCGGTCA GCCTCCGTTT CTGGCCACAA CTCCTCTGGA GACGCAGATG 
AAGGTGATCC GCTGGCAGAC GTCACTCCAC ATCCCACTGC AGGCCAAGCT CAGCCCGGAG 
GCCACAGACC TGATCCTGAA GCTGTGCCGT GGCCCGGATG ACCGGCTGGG CAGAAACGGA 
GCCGATGAGA TCAAAGCACA GCCCTTCTTC AGGACTATCG ACCTCTCCAA GGACCTGCGG 
CAGCAACATC AAGCCCCCTA CATCCCCAAA ATCACACACT CCACCGACAC CTCCAACTTC 
GACCCAGTGG ACCCGGACAA ACTGTGGAGC GAGGACGCGG ACGGAAATCA CAACGACACG 
CTCACCCGCT GGTTCAAGAA CGGCAAGCAC CCCGAGCACG CCTTCTACGA GTTCACCTTC 
CGCCGCTTTT TCGACGACAG CGGCCACCCG TACAGCTGCC CCAAGCCCAT CGAGAGCGAG 
GAGGAGGACG AGGACGAAGA CTACGTGGAA GAGGACGAGC CGTCGCAGCG GCCGATGGAG 
CAGAGCCGAG ATCTTGTGTA CGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lats1 ( XM_005160312.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160369.1 Danio rerio large tumor suppressor kinase 1 (lats1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24565
Clone ID Related Accession (Same CDS sequence)	XM_005160312.4 , XM_005160312.3
Accession Version	XM_005160312.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase LATS1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160312.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGGG CCGAGAAACC CGAGGGATAC AGACAAATGC GACCCAAGAC GTTCCCTGCC 
AGCAACAACA GCGGCAACAG TCAGCAAATG CTGCAGGAGA TCCGAGAGAG CCTGCGAAAC 
CTCTCCCCGT CCTCCAATGC GTCCAAGCCG GAGCAGAGCT CAGGGAAGGG CCCGCCGGAT 
GAGTCCAGAC AGCAAGGGCG CAGCACCAAC CCCAGAAACT CCCACCACCA CAGAGCCCTG 
CAGGAGATCC GCAAATCCCT GCAGCCGTTT GCCCACGACA GCTCCTCCAG CGGAGACAAC 
AGCTCCGCAG ACAGCAGCAA ACACACACTC CAGACGCAGG AGCCCGGCTT TGATGAGGAG 
CTGATGGTCA ACAGGATAAA CTTCCAGGAG GCTGTGAGGG AGCAGATCCC TTCGGCACGT 
CCTGTGAACA CGGCCATAAA ACAAGCGGGT CCACCCCAAA TTCAACAGCC ATTGTTGCGG 
CGGCAAAGCT GGAAGGGTTC AAAAGAGTCT CTGGCCCCAC AGCGACACAG CGCTTTAATT 
AATGATGTCC TCATTTATCG CCCCAGCAGC CCCGGGCCTC AAAGTGACCT CTCTCGGCCT 
GCCACCTTCC CTCAAAATCT GGCATCTGCC TCCAGCGGCC AGCGAGTGAA CGCTCCCCTG 
TCCCGGCAGG TGCGCAGCAT CACTCCTCCT CCGTCTTCAT GGGACTCCAA TCCCTCAACC 
AAGCGCTATT CTGGGAACTT GGATTACCTG GTGCCCCGGA TCTCCCCAGT GCCCCAAGGC 
CCTCGAGGTG ATGGCTATAT AAATCCTCAG TCCCAAAATC AGCGCGGGCT CAGCCCGGTG 
CCGGTGGGTC GGCAGCCCAT CATCATGCAG AACTCTAACA GCAACAAGTT TAACTTCCCA 
CCATCCTGGC CTCAGAACGG CAGCATGCAA AACGAATACA TGGGCATGAA CAGTGGTGCC 
CGGCAGCCAC CGCCACCTCC CTATTCCATG CACCAAACCA ACAGGCAGAG CCCCACAGCC 
CAGCAGATGC AGTCCAGCGC TCTTACTGCA CCTCCAAACG GAGCCAGCCT CCCTCAGAGC 
ATGCTGGTGC CCAACCGCAA CAGCCACAAC CTGGACATGT ACAACCTTGG AGTAATACGG 
CCGCCAGTGC AGCAGGCTCA GACGCCCCCC GGCCAGGACA CCTCCTCATC ATGGACGCAC 
AGCGTCCCAG GACGCTCCAA CTCTTTCACA AGTGCACCAA ACAATCGACG CCAGTGTCCC 
AACTCACAGC TCTCTGCCAC CACAGTCACC ACAGTAACCC AGGCGCCCAT CTTGCAGCCT 
GTCAAGAGCA TGCGTGTGCA GAAGCCGGAG CTGCACACCG CTGTGGCCCC TGCGCACCCG 
CCCTGGCTGC AGCCACCTCC GCCCACTGCT TACCAAGAGC CGCCCACGCC CCCCGCACCC 
GCTGCTGAAG TCCCGAGCTA CCAGGGCCCA CCGCCTCCCT ATCCCAAACA CCTGCTCCAG 
CAGCAGCAGC CGCCACCAGG GTACGACTCC AGCCCCGCAC TAAAGCCCTG CAGTAAAGAA 
GGGGACGCGG CAGACAACGA AAACGACACA GACAGCACCT GCTCCAGCGA GAGGGCCGAG 
GGCTCGGAGG AAGAGAAGAA GCAGATCACG ACGTCCCCGG TGCCGGTGCG CCGTCACAAA 
CGAGACGAGG AACGCAAGGG CGAAAGCAGA CTTCCCATGT ACTCTCCGCA GGCCTTCAAG 
TTCTTCATGG AGCAGCACGT GGAGAACATC CTGAAGAACC ACCAGCAGAG GATGCGCAGG 
AAGAAGCAGC TGGAGAGCGA GATGCAGCGG GTGGGACTCT CGGGCGACGC ACAGGAGCAG 
ATGCGCATGA TGCTGAGCCA GAAGGAGTCC AACTACATCC GCCTCAAGCG CGCCAAAATG 
GACAAATGCA TGTTCGAGAA GATCAAGACT CTGGGAATCG GGGCGTTTGG AGAAGTGTGT 
TTGGCTCGGA AAGTGGACAC TGGAGCTCTG TACGCCATGA AGACCCTCCG TAAAAAAGAC 
GTGTTGTTAA GGAACCAGGT AGCCCACGTG AAAGCCGAGC GGGATATTCT CGCTGAAGCG 
GATAACGAGT GGGTCGTCCG CTTGTACTAC TCTTTCCAGG ATAAAGATAA CTTGTACTTT 
GTCATGGACT ACATCCCTGG AGGAGACATG ATGAGTCTTC TGATCCGAAT GGGCATTTTT 
CAGGAGGACT TGGCTCAGTT TTACATCGCA GAGCTCATCT GTGCTGTGGA AAGCGTCCAC 
AAAATGGGTT TCATCCATCG AGACATCAAA CCTGACAACA TCCTGATCGA CCGTGATGGA 
CACATCAAAC TGACAGACTT CGGCCTCTGC ACTGGATTCA GATGGACTCA CGACTCGAAA 
TACTACCAAA GCGGTGATCA TGTGCGTCAG GACAGCATGG ACTTCAGTAT GGAGTGGGAA 
GATTCTGCAA ACTGCCGCTG CGGGGATCGC CTGAAGCCCC TGGAGCGCAG AGCGGCTAGA 
CAACACCAGC GCTGCCTGGC ACACTCTCTA GTGGGAACGC CCAATTACAT CGCACCCGAG 
GTTCTGCTGC GCACCGGCTA CACGCAGCTG TGTGATTGGT GGAGTGTTGG CGTTATCCTC 
TATGAGATGG TCGTCGGTCA GCCTCCGTTT CTGGCCACAA CTCCTCTGGA GACGCAGATG 
AAGGTGATCC GCTGGCAGAC GTCACTCCAC ATCCCACTGC AGGCCAAGCT CAGCCCGGAG 
GCCACAGACC TGATCCTGAA GCTGTGCCGT GGCCCGGATG ACCGGCTGGG CAGAAACGGA 
GCCGATGAGA TCAAAGCACA GCCCTTCTTC AGGACTATCG ACTTCTCCAA GGACCTGCGG 
CAGCAACATC AAGCCCCCTA CATCCCCAAA ATCACACACT CCACCGACAC CTCCAACTTC 
GACCCAGTGG ACCCGGACAA ACTGTGGAGC GAGGACGCGG ACGGAAACCA CAACGACACG 
CTCACCCGCT GGTTCAAGAA CGGCAAGCAC CCCGAGCACG CCTTCTACGA GTTCACCTTC 
CGCCGCTTTT TCGACGACAA CGGCCACCCG TACAGCTGCC CCAAGCCCAT CGAGAGCGAG 
GAGGAGGACG AGGACGAAGA CTACGTGGAA GAGGACGAGC CGTCGCAGCG GCCGATGGAG 
CAGAGCCGAG ATCTTGTGTA TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160369.1
CDS	249..3455
Translation	MRRAEKPEGYRQMRPKTFPASNNSGNSQQMLQEIRESLRNLSPSSNASKPEQSSGKGPPDESRQQGRSTNPRNSHHHRALQEIRKSLQPFAHDSSSSGDNSSADSSKHTLQTQEPGFDEELMVNRINFQEAVREQIPSARPVNTAIKQAGPPQIQQPLLRRQSWKGSKESLAPQRHSALINDVLIYRPSSPGPQSDLSRPATFPQNLASASSGQRVNAPLSRQVRSITPPPSSWDSNPSTKRYSGNLDYLVPRISPVPQGPRGDGYINPQSQNQRGLSPVPVGRQPIIMQNSNSNKFNFPPSWPQNGSMQNEYMGMNSGARQPPPPPYSMHQTNRQSPTAQQMQSSALTAPPNGASLPQSMLVPNRNSHNLDMYNLGVIRPPVQQAQTPPGQDTSSSWTHSVPGRSNSFTSAPNNRRQCPNSQLSATTVTTVTQAPILQPVKSMRVQKPELHTAVAPAHPPWLQPPPPTAYQEPPTPPAPAAEVPSYQGPPPPYPKHLLQQQQPPPGYDSSPALKPCSKEGDAADNENDTDSTCSSERAEGSEEEKKQITTSPVPVRRHKRDEERKGESRLPMYSPQAFKFFMEQHVENILKNHQQRMRRKKQLESEMQRVGLSGDAQEQMRMMLSQKESNYIRLKRAKMDKCMFEKIKTLGIGAFGEVCLARKVDTGALYAMKTLRKKDVLLRNQVAHVKAERDILAEADNEWVVRLYYSFQDKDNLYFVMDYIPGGDMMSLLIRMGIFQEDLAQFYIAELICAVESVHKMGFIHRDIKPDNILIDRDGHIKLTDFGLCTGFRWTHDSKYYQSGDHVRQDSMDFSMEWEDSANCRCGDRLKPLERRAARQHQRCLAHSLVGTPNYIAPEVLLRTGYTQLCDWWSVGVILYEMVVGQPPFLATTPLETQMKVIRWQTSLHIPLQAKLSPEATDLILKLCRGPDDRLGRNGADEIKAQPFFRTIDFSKDLRQQHQAPYIPKITHSTDTSNFDPVDPDKLWSEDADGNHNDTLTRWFKNGKHPEHAFYEFTFRRFFDDNGHPYSCPKPIESEEEDEDEDYVEEDEPSQRPMEQSRDLVYV

Target ORF information:

RefSeq Version	XM_005160312.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio large tumor suppressor kinase 1 (lats1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160312.4

ORF Insert Sequence:

ATGAGGAGGG CCGAGAAACC CGAGGGATAC AGACAAATGC GACCCAAGAC GTTCCCTGCC 
AGCAACAACA GCGGCAACAG TCAGCAAATG CTGCAGGAGA TCCGAGAGAG CCTGCGAAAC 
CTCTCCCCGT CCTCCAATGC GTCCAAGCCG GAGCAGAGCT CAGGGAAGGG CCCGCCGGAT 
GAGTCCAGAC AGCAAGGGCG CAGCACCAAC CCCAGAAACT CCCACCACCA CAGAGCCCTG 
CAGGAGATCC GCAAATCCCT GCAGCCGTTT GCCCACGACA GCTCCTCCAG CGGAGACAAC 
AGCTCCGCAG ACAGCAGCAA ACACACACTC CAGACGCAGG AGCCCGGCTT TGATGAGGAG 
CTGATGGTCA ACAGGATAAA CTTCCAGGAG GCTGTGAGGG AGCAGATCCC TTCGGCACGT 
CCTGTGAACA CGGCCATAAA ACAAGCGGGT CCACCCCAAA TTCAACAGCC ATTGTTGCGG 
CGGCAAAGCT GGAAGGGTTC AAAAGAGTCT CTGGCCCCAC AGCGACACAG CGCTTTAATT 
AATGATGTCC TCATTTATCG CCCCAGCAGC CCCGGGCCTC AAAGTGACCT CTCTCGGCCT 
GCCACCTTCC CTCAAAATCT GGCATCTGCC TCCAGCGGCC AGCGAGTGAA CGCTCCCCTG 
TCCCGGCAGG TGCGCAGCAT CACTCCTCCT CCGTCTTCAT GGGACTCCAA TCCCTCAACC 
AAGCGCTATT CTGGGAACTT GGATTACCTG GTGCCCCGGA TCTCCCCAGT GCCCCAAGGC 
CCTCGAGGTG ATGGCTATAT AAATCCTCAG TCCCAAAATC AGCGCGGGCT CAGCCCGGTG 
CCGGTGGGTC GGCAGCCCAT CATCATGCAG AACTCTAACA GCAACAAGTT TAACTTCCCA 
CCATCCTGGC CTCAGAACGG CAGCATGCAA AACGAATACA TGGGCATGAA CAGTGGTGCC 
CGGCAGCCAC CGCCACCTCC CTATTCCATG CACCAAACCA ACAGGCAGAG CCCCACAGCC 
CAGCAGATGC AGTCCAGCGC TCTTACTGCA CCTCCAAACG GAGCCAGCCT CCCTCAGAGC 
ATGCTGGTGC CCAACCGCAA CAGCCACAAC CTGGACATGT ACAACCTTGG AGTAATACGG 
CCGCCAGTGC AGCAGGCTCA GACGCCCCCC GGCCAGGACA CCTCCTCATC ATGGACGCAC 
AGCGTCCCAG GACGCTCCAA CTCTTTCACA AGTGCACCAA ACAATCGACG CCAGTGTCCC 
AACTCACAGC TCTCTGCCAC CACAGTCACC ACAGTAACCC AGGCGCCCAT CTTGCAGCCT 
GTCAAGAGCA TGCGTGTGCA GAAGCCGGAG CTGCACACCG CTGTGGCCCC TGCGCACCCG 
CCCTGGCTGC AGCCACCTCC GCCCACTGCT TACCAAGAGC CGCCCACGCC CCCCGCACCC 
GCTGCTGAAG TCCCGAGCTA CCAGGGCCCA CCGCCTCCCT ATCCCAAACA CCTGCTCCAG 
CAGCAGCAGC CGCCACCAGG GTACGACTCC AGCCCCGCAC TAAAGCCCTG CAGTAAAGAA 
GGGGACGCGG CAGACAACGA AAACGACACA GACAGCACCT GCTCCAGCGA GAGGGCCGAG 
GGCTCGGAGG AAGAGAAGAA GCAGATCACG ACGTCCCCGG TGCCGGTGCG CCGTCACAAA 
CGAGACGAGG AACGCAAGGG CGAAAGCAGA CTTCCCATGT ACTCTCCGCA GGCCTTCAAG 
TTCTTCATGG AGCAGCACGT GGAGAACATC CTGAAGAACC ACCAGCAGAG GATGCGCAGG 
AAGAAGCAGC TGGAGAGCGA GATGCAGCGG GTGGGACTCT CGGGCGACGC ACAGGAGCAG 
ATGCGCATGA TGCTGAGCCA GAAGGAGTCC AACTACATCC GCCTCAAGCG CGCCAAAATG 
GACAAATGCA TGTTCGAGAA GATCAAGACT CTGGGAATCG GGGCGTTTGG AGAAGTGTGT 
TTGGCTCGGA AAGTGGACAC TGGAGCTCTG TACGCCATGA AGACCCTCCG TAAAAAAGAC 
GTGTTGTTAA GGAACCAGGT AGCCCACGTG AAAGCCGAGC GGGATATTCT CGCTGAAGCG 
GATAACGAGT GGGTCGTCCG CTTGTACTAC TCTTTCCAGG ATAAAGATAA CTTGTACTTT 
GTCATGGACT ACATCCCTGG AGGAGACATG ATGAGTCTTC TGATCCGAAT GGGCATTTTT 
CAGGAGGACT TGGCTCAGTT TTACATCGCA GAGCTCATCT GTGCTGTGGA AAGCGTCCAC 
AAAATGGGTT TCATCCATCG AGACATCAAA CCTGACAACA TCCTGATCGA CCGTGATGGA 
CACATCAAAC TGACAGACTT CGGCCTCTGC ACTGGATTCA GATGGACTCA CGACTCGAAA 
TACTACCAAA GCGGTGATCA TGTGCGTCAG GACAGCATGG ACTTCAGTAT GGAGTGGGAA 
GATTCTGCAA ACTGCCGCTG CGGGGATCGC CTGAAGCCCC TGGAGCGCAG AGCGGCTAGA 
CAACACCAGC GCTGCCTGGC ACACTCTCTA GTGGGAACGC CCAATTACAT CGCACCCGAG 
GTTCTGCTGC GCACCGGCTA CACGCAGCTG TGTGATTGGT GGAGTGTTGG CGTTATCCTC 
TATGAGATGG TCGTCGGTCA GCCTCCGTTT CTGGCCACAA CTCCTCTGGA GACGCAGATG 
AAGGTGATCC GCTGGCAGAC GTCACTCCAC ATCCCACTGC AGGCCAAGCT CAGCCCGGAG 
GCCACAGACC TGATCCTGAA GCTGTGCCGT GGCCCGGATG ACCGGCTGGG CAGAAACGGA 
GCCGATGAGA TCAAAGCACA GCCCTTCTTC AGGACTATCG ACTTCTCCAA GGACCTGCGG 
CAGCAACATC AAGCCCCCTA CATCCCCAAA ATCACACACT CCACCGACAC CTCCAACTTC 
GACCCAGTGG ACCCGGACAA ACTGTGGAGC GAGGACGCGG ACGGAAACCA CAACGACACG 
CTCACCCGCT GGTTCAAGAA CGGCAAGCAC CCCGAGCACG CCTTCTACGA GTTCACCTTC 
CGCCGCTTTT TCGACGACAA CGGCCACCCG TACAGCTGCC CCAAGCCCAT CGAGAGCGAG 
GAGGAGGACG AGGACGAAGA CTACGTGGAA GAGGACGAGC CGTCGCAGCG GCCGATGGAG 
CAGAGCCGAG ATCTTGTGTA TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lats1 ( XM_005160312.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160369.1 Danio rerio large tumor suppressor kinase 1 (lats1), transcript variant X1, mRNA.

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CloneID	ODa24565
Clone ID Related Accession (Same CDS sequence)	XM_005160312.4 , XM_005160312.3
Accession Version	XM_005160312.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase LATS1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160312.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGGG CCGAGAAACC CGAGGGATAC AGACAAATGC GACCCAAGAC GTTCCCTGCC 
AGCAACAACA GCGGCAACAG TCAGCAAATG CTGCAGGAGA TCCGAGAGAG CCTGCGAAAC 
CTCTCCCCGT CCTCCAATGC GTCCAAGCCG GAGCAGAGCT CAGGGAAGGG CCCGCCGGAT 
GAGTCCAGAC AGCAAGGGCG CAGCACCAAC CCCAGAAACT CCCACCACCA CAGAGCCCTG 
CAGGAGATCC GCAAATCCCT GCAGCCGTTT GCCCACGACA GCTCCTCCAG CGGAGACAAC 
AGCTCCGCAG ACAGCAGCAA ACACACACTC CAGACGCAGG AGCCCGGCTT TGATGAGGAG 
CTGATGGTCA ACAGGATAAA CTTCCAGGAG GCTGTGAGGG AGCAGATCCC TTCGGCACGT 
CCTGTGAACA CGGCCATAAA ACAAGCGGGT CCACCCCAAA TTCAACAGCC ATTGTTGCGG 
CGGCAAAGCT GGAAGGGTTC AAAAGAGTCT CTGGCCCCAC AGCGACACAG CGCTTTAATT 
AATGATGTCC TCATTTATCG CCCCAGCAGC CCCGGGCCTC AAAGTGACCT CTCTCGGCCT 
GCCACCTTCC CTCAAAATCT GGCATCTGCC TCCAGCGGCC AGCGAGTGAA CGCTCCCCTG 
TCCCGGCAGG TGCGCAGCAT CACTCCTCCT CCGTCTTCAT GGGACTCCAA TCCCTCAACC 
AAGCGCTATT CTGGGAACTT GGATTACCTG GTGCCCCGGA TCTCCCCAGT GCCCCAAGGC 
CCTCGAGGTG ATGGCTATAT AAATCCTCAG TCCCAAAATC AGCGCGGGCT CAGCCCGGTG 
CCGGTGGGTC GGCAGCCCAT CATCATGCAG AACTCTAACA GCAACAAGTT TAACTTCCCA 
CCATCCTGGC CTCAGAACGG CAGCATGCAA AACGAATACA TGGGCATGAA CAGTGGTGCC 
CGGCAGCCAC CGCCACCTCC CTATTCCATG CACCAAACCA ACAGGCAGAG CCCCACAGCC 
CAGCAGATGC AGTCCAGCGC TCTTACTGCA CCTCCAAACG GAGCCAGCCT CCCTCAGAGC 
ATGCTGGTGC CCAACCGCAA CAGCCACAAC CTGGACATGT ACAACCTTGG AGTAATACGG 
CCGCCAGTGC AGCAGGCTCA GACGCCCCCC GGCCAGGACA CCTCCTCATC ATGGACGCAC 
AGCGTCCCAG GACGCTCCAA CTCTTTCACA AGTGCACCAA ACAATCGACG CCAGTGTCCC 
AACTCACAGC TCTCTGCCAC CACAGTCACC ACAGTAACCC AGGCGCCCAT CTTGCAGCCT 
GTCAAGAGCA TGCGTGTGCA GAAGCCGGAG CTGCACACCG CTGTGGCCCC TGCGCACCCG 
CCCTGGCTGC AGCCACCTCC GCCCACTGCT TACCAAGAGC CGCCCACGCC CCCCGCACCC 
GCTGCTGAAG TCCCGAGCTA CCAGGGCCCA CCGCCTCCCT ATCCCAAACA CCTGCTCCAG 
CAGCAGCAGC CGCCACCAGG GTACGACTCC AGCCCCGCAC TAAAGCCCTG CAGTAAAGAA 
GGGGACGCGG CAGACAACGA AAACGACACA GACAGCACCT GCTCCAGCGA GAGGGCCGAG 
GGCTCGGAGG AAGAGAAGAA GCAGATCACG ACGTCCCCGG TGCCGGTGCG CCGTCACAAA 
CGAGACGAGG AACGCAAGGG CGAAAGCAGA CTTCCCATGT ACTCTCCGCA GGCCTTCAAG 
TTCTTCATGG AGCAGCACGT GGAGAACATC CTGAAGAACC ACCAGCAGAG GATGCGCAGG 
AAGAAGCAGC TGGAGAGCGA GATGCAGCGG GTGGGACTCT CGGGCGACGC ACAGGAGCAG 
ATGCGCATGA TGCTGAGCCA GAAGGAGTCC AACTACATCC GCCTCAAGCG CGCCAAAATG 
GACAAATGCA TGTTCGAGAA GATCAAGACT CTGGGAATCG GGGCGTTTGG AGAAGTGTGT 
TTGGCTCGGA AAGTGGACAC TGGAGCTCTG TACGCCATGA AGACCCTCCG TAAAAAAGAC 
GTGTTGTTAA GGAACCAGGT AGCCCACGTG AAAGCCGAGC GGGATATTCT CGCTGAAGCG 
GATAACGAGT GGGTCGTCCG CTTGTACTAC TCTTTCCAGG ATAAAGATAA CTTGTACTTT 
GTCATGGACT ACATCCCTGG AGGAGACATG ATGAGTCTTC TGATCCGAAT GGGCATTTTT 
CAGGAGGACT TGGCTCAGTT TTACATCGCA GAGCTCATCT GTGCTGTGGA AAGCGTCCAC 
AAAATGGGTT TCATCCATCG AGACATCAAA CCTGACAACA TCCTGATCGA CCGTGATGGA 
CACATCAAAC TGACAGACTT CGGCCTCTGC ACTGGATTCA GATGGACTCA CGACTCGAAA 
TACTACCAAA GCGGTGATCA TGTGCGTCAG GACAGCATGG ACTTCAGTAT GGAGTGGGAA 
GATTCTGCAA ACTGCCGCTG CGGGGATCGC CTGAAGCCCC TGGAGCGCAG AGCGGCTAGA 
CAACACCAGC GCTGCCTGGC ACACTCTCTA GTGGGAACGC CCAATTACAT CGCACCCGAG 
GTTCTGCTGC GCACCGGCTA CACGCAGCTG TGTGATTGGT GGAGTGTTGG CGTTATCCTC 
TATGAGATGG TCGTCGGTCA GCCTCCGTTT CTGGCCACAA CTCCTCTGGA GACGCAGATG 
AAGGTGATCC GCTGGCAGAC GTCACTCCAC ATCCCACTGC AGGCCAAGCT CAGCCCGGAG 
GCCACAGACC TGATCCTGAA GCTGTGCCGT GGCCCGGATG ACCGGCTGGG CAGAAACGGA 
GCCGATGAGA TCAAAGCACA GCCCTTCTTC AGGACTATCG ACTTCTCCAA GGACCTGCGG 
CAGCAACATC AAGCCCCCTA CATCCCCAAA ATCACACACT CCACCGACAC CTCCAACTTC 
GACCCAGTGG ACCCGGACAA ACTGTGGAGC GAGGACGCGG ACGGAAACCA CAACGACACG 
CTCACCCGCT GGTTCAAGAA CGGCAAGCAC CCCGAGCACG CCTTCTACGA GTTCACCTTC 
CGCCGCTTTT TCGACGACAA CGGCCACCCG TACAGCTGCC CCAAGCCCAT CGAGAGCGAG 
GAGGAGGACG AGGACGAAGA CTACGTGGAA GAGGACGAGC CGTCGCAGCG GCCGATGGAG 
CAGAGCCGAG ATCTTGTGTA TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160369.1
CDS	252..3458
Translation	MRRAEKPEGYRQMRPKTFPASNNSGNSQQMLQEIRESLRNLSPSSNASKPEQSSGKGPPDESRQQGRSTNPRNSHHHRALQEIRKSLQPFAHDSSSSGDNSSADSSKHTLQTQEPGFDEELMVNRINFQEAVREQIPSARPVNTAIKQAGPPQIQQPLLRRQSWKGSKESLAPQRHSALINDVLIYRPSSPGPQSDLSRPATFPQNLASASSGQRVNAPLSRQVRSITPPPSSWDSNPSTKRYSGNLDYLVPRISPVPQGPRGDGYINPQSQNQRGLSPVPVGRQPIIMQNSNSNKFNFPPSWPQNGSMQNEYMGMNSGARQPPPPPYSMHQTNRQSPTAQQMQSSALTAPPNGASLPQSMLVPNRNSHNLDMYNLGVIRPPVQQAQTPPGQDTSSSWTHSVPGRSNSFTSAPNNRRQCPNSQLSATTVTTVTQAPILQPVKSMRVQKPELHTAVAPAHPPWLQPPPPTAYQEPPTPPAPAAEVPSYQGPPPPYPKHLLQQQQPPPGYDSSPALKPCSKEGDAADNENDTDSTCSSERAEGSEEEKKQITTSPVPVRRHKRDEERKGESRLPMYSPQAFKFFMEQHVENILKNHQQRMRRKKQLESEMQRVGLSGDAQEQMRMMLSQKESNYIRLKRAKMDKCMFEKIKTLGIGAFGEVCLARKVDTGALYAMKTLRKKDVLLRNQVAHVKAERDILAEADNEWVVRLYYSFQDKDNLYFVMDYIPGGDMMSLLIRMGIFQEDLAQFYIAELICAVESVHKMGFIHRDIKPDNILIDRDGHIKLTDFGLCTGFRWTHDSKYYQSGDHVRQDSMDFSMEWEDSANCRCGDRLKPLERRAARQHQRCLAHSLVGTPNYIAPEVLLRTGYTQLCDWWSVGVILYEMVVGQPPFLATTPLETQMKVIRWQTSLHIPLQAKLSPEATDLILKLCRGPDDRLGRNGADEIKAQPFFRTIDFSKDLRQQHQAPYIPKITHSTDTSNFDPVDPDKLWSEDADGNHNDTLTRWFKNGKHPEHAFYEFTFRRFFDDNGHPYSCPKPIESEEEDEDEDYVEEDEPSQRPMEQSRDLVYV

Target ORF information:

RefSeq Version	XM_005160312.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio large tumor suppressor kinase 1 (lats1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160312.3

ORF Insert Sequence:

ATGAGGAGGG CCGAGAAACC CGAGGGATAC AGACAAATGC GACCCAAGAC GTTCCCTGCC 
AGCAACAACA GCGGCAACAG TCAGCAAATG CTGCAGGAGA TCCGAGAGAG CCTGCGAAAC 
CTCTCCCCGT CCTCCAATGC GTCCAAGCCG GAGCAGAGCT CAGGGAAGGG CCCGCCGGAT 
GAGTCCAGAC AGCAAGGGCG CAGCACCAAC CCCAGAAACT CCCACCACCA CAGAGCCCTG 
CAGGAGATCC GCAAATCCCT GCAGCCGTTT GCCCACGACA GCTCCTCCAG CGGAGACAAC 
AGCTCCGCAG ACAGCAGCAA ACACACACTC CAGACGCAGG AGCCCGGCTT TGATGAGGAG 
CTGATGGTCA ACAGGATAAA CTTCCAGGAG GCTGTGAGGG AGCAGATCCC TTCGGCACGT 
CCTGTGAACA CGGCCATAAA ACAAGCGGGT CCACCCCAAA TTCAACAGCC ATTGTTGCGG 
CGGCAAAGCT GGAAGGGTTC AAAAGAGTCT CTGGCCCCAC AGCGACACAG CGCTTTAATT 
AATGATGTCC TCATTTATCG CCCCAGCAGC CCCGGGCCTC AAAGTGACCT CTCTCGGCCT 
GCCACCTTCC CTCAAAATCT GGCATCTGCC TCCAGCGGCC AGCGAGTGAA CGCTCCCCTG 
TCCCGGCAGG TGCGCAGCAT CACTCCTCCT CCGTCTTCAT GGGACTCCAA TCCCTCAACC 
AAGCGCTATT CTGGGAACTT GGATTACCTG GTGCCCCGGA TCTCCCCAGT GCCCCAAGGC 
CCTCGAGGTG ATGGCTATAT AAATCCTCAG TCCCAAAATC AGCGCGGGCT CAGCCCGGTG 
CCGGTGGGTC GGCAGCCCAT CATCATGCAG AACTCTAACA GCAACAAGTT TAACTTCCCA 
CCATCCTGGC CTCAGAACGG CAGCATGCAA AACGAATACA TGGGCATGAA CAGTGGTGCC 
CGGCAGCCAC CGCCACCTCC CTATTCCATG CACCAAACCA ACAGGCAGAG CCCCACAGCC 
CAGCAGATGC AGTCCAGCGC TCTTACTGCA CCTCCAAACG GAGCCAGCCT CCCTCAGAGC 
ATGCTGGTGC CCAACCGCAA CAGCCACAAC CTGGACATGT ACAACCTTGG AGTAATACGG 
CCGCCAGTGC AGCAGGCTCA GACGCCCCCC GGCCAGGACA CCTCCTCATC ATGGACGCAC 
AGCGTCCCAG GACGCTCCAA CTCTTTCACA AGTGCACCAA ACAATCGACG CCAGTGTCCC 
AACTCACAGC TCTCTGCCAC CACAGTCACC ACAGTAACCC AGGCGCCCAT CTTGCAGCCT 
GTCAAGAGCA TGCGTGTGCA GAAGCCGGAG CTGCACACCG CTGTGGCCCC TGCGCACCCG 
CCCTGGCTGC AGCCACCTCC GCCCACTGCT TACCAAGAGC CGCCCACGCC CCCCGCACCC 
GCTGCTGAAG TCCCGAGCTA CCAGGGCCCA CCGCCTCCCT ATCCCAAACA CCTGCTCCAG 
CAGCAGCAGC CGCCACCAGG GTACGACTCC AGCCCCGCAC TAAAGCCCTG CAGTAAAGAA 
GGGGACGCGG CAGACAACGA AAACGACACA GACAGCACCT GCTCCAGCGA GAGGGCCGAG 
GGCTCGGAGG AAGAGAAGAA GCAGATCACG ACGTCCCCGG TGCCGGTGCG CCGTCACAAA 
CGAGACGAGG AACGCAAGGG CGAAAGCAGA CTTCCCATGT ACTCTCCGCA GGCCTTCAAG 
TTCTTCATGG AGCAGCACGT GGAGAACATC CTGAAGAACC ACCAGCAGAG GATGCGCAGG 
AAGAAGCAGC TGGAGAGCGA GATGCAGCGG GTGGGACTCT CGGGCGACGC ACAGGAGCAG 
ATGCGCATGA TGCTGAGCCA GAAGGAGTCC AACTACATCC GCCTCAAGCG CGCCAAAATG 
GACAAATGCA TGTTCGAGAA GATCAAGACT CTGGGAATCG GGGCGTTTGG AGAAGTGTGT 
TTGGCTCGGA AAGTGGACAC TGGAGCTCTG TACGCCATGA AGACCCTCCG TAAAAAAGAC 
GTGTTGTTAA GGAACCAGGT AGCCCACGTG AAAGCCGAGC GGGATATTCT CGCTGAAGCG 
GATAACGAGT GGGTCGTCCG CTTGTACTAC TCTTTCCAGG ATAAAGATAA CTTGTACTTT 
GTCATGGACT ACATCCCTGG AGGAGACATG ATGAGTCTTC TGATCCGAAT GGGCATTTTT 
CAGGAGGACT TGGCTCAGTT TTACATCGCA GAGCTCATCT GTGCTGTGGA AAGCGTCCAC 
AAAATGGGTT TCATCCATCG AGACATCAAA CCTGACAACA TCCTGATCGA CCGTGATGGA 
CACATCAAAC TGACAGACTT CGGCCTCTGC ACTGGATTCA GATGGACTCA CGACTCGAAA 
TACTACCAAA GCGGTGATCA TGTGCGTCAG GACAGCATGG ACTTCAGTAT GGAGTGGGAA 
GATTCTGCAA ACTGCCGCTG CGGGGATCGC CTGAAGCCCC TGGAGCGCAG AGCGGCTAGA 
CAACACCAGC GCTGCCTGGC ACACTCTCTA GTGGGAACGC CCAATTACAT CGCACCCGAG 
GTTCTGCTGC GCACCGGCTA CACGCAGCTG TGTGATTGGT GGAGTGTTGG CGTTATCCTC 
TATGAGATGG TCGTCGGTCA GCCTCCGTTT CTGGCCACAA CTCCTCTGGA GACGCAGATG 
AAGGTGATCC GCTGGCAGAC GTCACTCCAC ATCCCACTGC AGGCCAAGCT CAGCCCGGAG 
GCCACAGACC TGATCCTGAA GCTGTGCCGT GGCCCGGATG ACCGGCTGGG CAGAAACGGA 
GCCGATGAGA TCAAAGCACA GCCCTTCTTC AGGACTATCG ACTTCTCCAA GGACCTGCGG 
CAGCAACATC AAGCCCCCTA CATCCCCAAA ATCACACACT CCACCGACAC CTCCAACTTC 
GACCCAGTGG ACCCGGACAA ACTGTGGAGC GAGGACGCGG ACGGAAACCA CAACGACACG 
CTCACCCGCT GGTTCAAGAA CGGCAAGCAC CCCGAGCACG CCTTCTACGA GTTCACCTTC 
CGCCGCTTTT TCGACGACAA CGGCCACCCG TACAGCTGCC CCAAGCCCAT CGAGAGCGAG 
GAGGAGGACG AGGACGAAGA CTACGTGGAA GAGGACGAGC CGTCGCAGCG GCCGATGGAG 
CAGAGCCGAG ATCTTGTGTA TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Phosphorylation of angiomotin by Lats1/2 kinases inhibits F-actin binding, cell migration, and angiogenesis.
The Journal of biological chemistry288(47)34041-51(2013 Nov)
Dai X,She P,Chi F,Feng Y,Liu H,Jin D,Zhao Y,Guo X,Jiang D,Guan KL,Zhong TP,Zhao B

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lats1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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