EIF2B4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	EIF2B4
Entrez Gene ID	541548
Full Name	eukaryotic translation initiation factor 2B, subunit 4 delta
Synonyms	si:ch211-101l18.4,wu:fb54a09,zgc:110790
General protein information	Preferred Names eukaryotic translation initiation factor 2B, subunit 4 delta Names translation initiation factor eIF-2B subunit delta
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005160460.4	XP_005160517.1	translation initiation factor eIF-2B subunit delta isoform X1
XM_005160461.3	XP_005160518.1	translation initiation factor eIF-2B subunit delta isoform X2
NM_001014361.2	NP_001014383.1	translation initiation factor eIF-2B subunit delta
XM_005160461.4	XP_005160518.1	translation initiation factor eIF-2B subunit delta isoform X2
XM_005160462.4	XP_005160519.1	translation initiation factor eIF-2B subunit delta isoform X3
XM_005160460.3	XP_005160517.1	translation initiation factor eIF-2B subunit delta isoform X1
XM_005160462.3	XP_005160519.1	translation initiation factor eIF-2B subunit delta isoform X3

Pathways from BioSystems

Homologies

	EIF2B4	XP_001094256.2
Gallus gallus (chicken)	EIF2B4	XP_423512.2
Pan troglodytes (chimpanzee)	EIF2B4	XP_515356.2
Rattus norvegicus (Norway rat)	Eif2b4	NP_446402.1
Xenopus tropicalis (tropical clawed frog)	eif2b4	NP_001072364.1
Homo sapiens (human)	EIF2B4	NP_001029288.1
Bos taurus (cattle)	EIF2B4	NP_001029716.1
Mus musculus (house mouse)	Eif2b4	NP_034252.2
Danio rerio (zebrafish)	eif2b4	NP_001014383.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005937	XP_556607.3
Canis lupus familiaris (dog)	EIF2B4	XP_860642.1
Arabidopsis thaliana (thale cress)	AT5G38640	NP_198680.1
Oryza sativa (rice)	Os12g0617100	NP_001067287.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Evolution and genomic organization of muscle microRNAs in fish genomes.
Nachtigall PG, Dias MC, Pinhal D
BMC evolutionary biology14196(2014 Sep)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EIF2B4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF2B4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa30621	NM_001014361.2 Latest version!	Danio rerio eukaryotic translation initiation factor 2B, subunit 4 delta (eif2b4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa24732	XM_005160460.4 Latest version!	Danio rerio eukaryotic translation initiation factor 2B, subunit 4 delta (eif2b4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa24733	XM_005160461.4 Latest version!	Danio rerio eukaryotic translation initiation factor 2B, subunit 4 delta (eif2b4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa24734	XM_005160462.4 Latest version!	Danio rerio eukaryotic translation initiation factor 2B, subunit 4 delta (eif2b4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa24733	XM_005160461.3	Danio rerio eukaryotic translation initiation factor 2B, subunit 4 delta (eif2b4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa24732	XM_005160460.3	Danio rerio eukaryotic translation initiation factor 2B, subunit 4 delta (eif2b4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa24734	XM_005160462.3	Danio rerio eukaryotic translation initiation factor 2B, subunit 4 delta (eif2b4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EIF2B4 ( NM_001014361.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001014383.1 Danio rerio eukaryotic translation initiation factor 2B, subunit 4 delta (eif2b4), mRNA.

Price & Availability↑

CloneID	ODa30621
Clone ID Related Accession (Same CDS sequence)	NM_001014361.2
Accession Version	NM_001014361.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	translation initiation factor eIF-2B subunit delta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC091878.1. On Aug 30, 2012 this sequence version replaced NM_001014361.1. ##Evidence-Data-START## Transcript exon combination :: BC091878.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGACT CGGATGGGAG ACAGGTTGAC AGCATCAACC ATGCTACAGG TGAAGAAGGG 
AAGTTGCAAG ATATTGTAAA AGCAAAGAGT GAAGGTAAGG AGTTGACCAA AGAAGAAAAG 
CAGCGTCTTA GGAAAGAGAA GAAGCAGCAT AAAAAGAATA AAAAAGATGA CAACAAGGGT 
CCTCCAGAGA AGGAAAAGGA GAAGGAAAAA GAGAAAGCAT CTGCTCCACC TCCATCAAAG 
CCTGTCACCC AGAGCCCGGC ACATAAAGCT GCTCCAGCAC CTGTAACTGC ATCTGCATCT 
GAAACTCCTG CGGCTGCAGA GAAGCCTGCA AAGACCAAAG CAGAGCTGCG AGCAGAGCGA 
CGGGCACGGC AAGACTCAGA ACGAGCATCA AAACAGGGCA AGAAGGAAGG TGGTCAACAA 
TCCACCTCCA GCAAACCTAA AGCCTCCCCC AATGAGCTCC AGCCAGTTGT GAAAAGGTTA 
CCTGAGCACG TTCAGGTGGA TGATCCTGCT GCGTTGAAAA AGTTGGCCAA GAAGCTTGAG 
CGACAGCAGG TTCCTTTGAG GTCAGATTAT GGTTACAAGG TCAGCCTGTT CTCACATCTA 
CATCAATACA GCCGAAAAAA CCCTCCCACG CAGCAAATCA GCATCCCAGC CACAGTTATT 
CACCCATCCA TTGTTCGGTT GGGCCTGCAG TACTCTCAGG GCATTATAGC AGGATCCAAT 
GCTCGATCTG TGGCCCTGCT ACATGCCTTC AAACAGGTTA TTCAAGACTA TAGCACACCT 
CCAAACGAGG AGTTGTCAAG GGACCTTGTG AACAAGCTTT CACCTTATAT CAGTTTCCTC 
AGCCAGTGTC GGCCTCTGTC TGCCAGCATG GGCAATGCCA TCAAGTACAT AAAGAAGGAG 
ATTTCAAATA TACCCAATCA ATGCAAAGAA GAGGAGGCAA AAGGGAAACT GCAGGCATGT 
ATTGATAGTT ACATAAATGA GAAGATCATT TTGGCTTCTG AAGCCATTTC TAAATATGCT 
ATTGAGAAGA TAAGCAATGG AGACGTCATT CTAGTTTACG GCTGCTCGTC ACTTGTCAAT 
CACATTCTGT GCGAAGCCTT TGAGAAGCAG AGGCAGTTCA GAGTCATAGT TGTGGACAGT 
CGGCCCAGGT TGGAAGGTCG AGAGGCGCTG AGGCGTCTGG TGAAGAAAGG CATCCGATGT 
ACTTACGTGC TCATTTCTGC CTTGTCCTAC ATTCTGCCTG AAGTATCCAA GGTATTTCTC 
GGTGCTCATG CACTCCTTGC CAATGGATAT GTGATGTCAC GTGTTGGAAC TTCCCAAATT 
GCCCTGGTAG CAAAGGCCTA CAATGTACCA GTGCTGGTCT GCTGTGAGAC CTACAAGTTC 
TGTGAACGAG TGCAGACGGA CTCTTTTGTA TCCAATGAAT TAGATGACCC TGATGACCTG 
ATTGTGACCA GAAATGGGAA GACCCACCTA GAGAACTGGC AGACTGTTAA ATCGCTGGGC 
CTTCTGAACC TGGTGTATGA TGTGACGCCA CCAGACTTTG TGGACCTTGT GATCACCGAT 
TTGGGCATGA TCCCCTGCAC CTCTGTGCCT GTGGTACTGC GAGTCAAGAA TGTAGATCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014383.1
CDS	9..1631
Translation	MADSDGRQVDSINHATGEEGKLQDIVKAKSEGKELTKEEKQRLRKEKKQHKKNKKDDNKGPPEKEKEKEKEKASAPPPSKPVTQSPAHKAAPAPVTASASETPAAAEKPAKTKAELRAERRARQDSERASKQGKKEGGQQSTSSKPKASPNELQPVVKRLPEHVQVDDPAALKKLAKKLERQQVPLRSDYGYKVSLFSHLHQYSRKNPPTQQISIPATVIHPSIVRLGLQYSQGIIAGSNARSVALLHAFKQVIQDYSTPPNEELSRDLVNKLSPYISFLSQCRPLSASMGNAIKYIKKEISNIPNQCKEEEAKGKLQACIDSYINEKIILASEAISKYAIEKISNGDVILVYGCSSLVNHILCEAFEKQRQFRVIVVDSRPRLEGREALRRLVKKGIRCTYVLISALSYILPEVSKVFLGAHALLANGYVMSRVGTSQIALVAKAYNVPVLVCCETYKFCERVQTDSFVSNELDDPDDLIVTRNGKTHLENWQTVKSLGLLNLVYDVTPPDFVDLVITDLGMIPCTSVPVVLRVKNVDQ

Target ORF information:

RefSeq Version	NM_001014361.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 2B, subunit 4 delta (eif2b4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014361.2

ORF Insert Sequence:

ATGGCGGACT CGGATGGGAG ACAGGTTGAC AGCATCAACC ATGCTACAGG TGAAGAAGGG 
AAGTTGCAAG ATATTGTAAA AGCAAAGAGT GAAGGTAAGG AGTTGACCAA AGAAGAAAAG 
CAGCGTCTTA GGAAAGAGAA GAAGCAGCAT AAAAAGAATA AAAAAGATGA CAACAAGGGT 
CCTCCAGAGA AGGAAAAGGA GAAGGAAAAA GAGAAAGCAT CTGCTCCACC TCCATCAAAG 
CCTGTCACCC AGAGCCCGGC ACATAAAGCT GCTCCAGCAC CTGTAACTGC ATCTGCATCT 
GAAACTCCTG CGGCTGCAGA GAAGCCTGCA AAGACCAAAG CAGAGCTGCG AGCAGAGCGA 
CGGGCACGGC AAGACTCAGA ACGAGCATCA AAACAGGGCA AGAAGGAAGG TGGTCAACAA 
TCCACCTCCA GCAAACCTAA AGCCTCCCCC AATGAGCTCC AGCCAGTTGT GAAAAGGTTA 
CCTGAGCACG TTCAGGTGGA TGATCCTGCT GCGTTGAAAA AGTTGGCCAA GAAGCTTGAG 
CGACAGCAGG TTCCTTTGAG GTCAGATTAT GGTTACAAGG TCAGCCTGTT CTCACATCTA 
CATCAATACA GCCGAAAAAA CCCTCCCACG CAGCAAATCA GCATCCCAGC CACAGTTATT 
CACCCATCCA TTGTTCGGTT GGGCCTGCAG TACTCTCAGG GCATTATAGC AGGATCCAAT 
GCTCGATCTG TGGCCCTGCT ACATGCCTTC AAACAGGTTA TTCAAGACTA TAGCACACCT 
CCAAACGAGG AGTTGTCAAG GGACCTTGTG AACAAGCTTT CACCTTATAT CAGTTTCCTC 
AGCCAGTGTC GGCCTCTGTC TGCCAGCATG GGCAATGCCA TCAAGTACAT AAAGAAGGAG 
ATTTCAAATA TACCCAATCA ATGCAAAGAA GAGGAGGCAA AAGGGAAACT GCAGGCATGT 
ATTGATAGTT ACATAAATGA GAAGATCATT TTGGCTTCTG AAGCCATTTC TAAATATGCT 
ATTGAGAAGA TAAGCAATGG AGACGTCATT CTAGTTTACG GCTGCTCGTC ACTTGTCAAT 
CACATTCTGT GCGAAGCCTT TGAGAAGCAG AGGCAGTTCA GAGTCATAGT TGTGGACAGT 
CGGCCCAGGT TGGAAGGTCG AGAGGCGCTG AGGCGTCTGG TGAAGAAAGG CATCCGATGT 
ACTTACGTGC TCATTTCTGC CTTGTCCTAC ATTCTGCCTG AAGTATCCAA GGTATTTCTC 
GGTGCTCATG CACTCCTTGC CAATGGATAT GTGATGTCAC GTGTTGGAAC TTCCCAAATT 
GCCCTGGTAG CAAAGGCCTA CAATGTACCA GTGCTGGTCT GCTGTGAGAC CTACAAGTTC 
TGTGAACGAG TGCAGACGGA CTCTTTTGTA TCCAATGAAT TAGATGACCC TGATGACCTG 
ATTGTGACCA GAAATGGGAA GACCCACCTA GAGAACTGGC AGACTGTTAA ATCGCTGGGC 
CTTCTGAACC TGGTGTATGA TGTGACGCCA CCAGACTTTG TGGACCTTGT GATCACCGAT 
TTGGGCATGA TCCCCTGCAC CTCTGTGCCT GTGGTACTGC GAGTCAAGAA TGTAGATCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2B4 ( XM_005160460.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160517.1 Danio rerio eukaryotic translation initiation factor 2B, subunit 4 delta (eif2b4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24732
Clone ID Related Accession (Same CDS sequence)	XM_005160460.4 , XM_005160460.3
Accession Version	XM_005160460.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	translation initiation factor eIF-2B subunit delta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160460.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACT CGGATGGGAG ACAGGTTGAC AGCATCAACC ATGCTACAGG TGAAGAAGGG 
AAGTTGCAAG ATATTGTAAA AGCAAAGAGT GAAGGTAAGG AGTTGACCAA AGAAGAAAAG 
CAGCGTCTTA GGAAAGAGAA GAAGCAGCAT AAAAAGAATA AAAAAGATGA CAACAAGGGT 
CCTCCAGAGA AGGAAAAGGA GAAGGAAAAA GAGAAAGCAT CTGCTCCACC TCCATCAAAG 
CCTGTCACCC AGAGCCCGGC ACATAAAGCA GCTGCTCCAG CACCTGTAAC TGCATCTGCA 
TCTGAAACTC CTGCGGCTGC AGAGAAGCCT GCAAAGACCA AAGCAGAGCT GCGAGCAGAG 
CGACGGGCAC GGCAAGACTC AGAACGAGCA TCAAAACAGG GCAAGAAGGA AGGTGGTCAA 
CAATCCACCT CCAGCAAACC TAAAGCCTCC CCCAATGAGC TCCAGCCAGT TGTGAAAAGG 
TTACCTGAGC ACGTTCAGGT GGATGATCCT GCTGCGTTGA AAAAGTTGGC CAAGAAGCTT 
GAGCGACAGC AGAAGTCAGG GCAGGGATGC GGTTTAAATA TAACTGGAGT CCAGGTTCCT 
TTGAGGTCAG ATTATGGTTA CAAGGTCAGC CTGTTCTCAC ATCTACATCA ATACAGCCGA 
AAAAACCCTC CCACGCAGCA AATCAGCATC CCAGCCACAG TTATTCACCC ATCCATTGTT 
CGGTTGGGCC TGCAGTACTC TCAGGGCATT ATAGCAGGAT CCAATGCTCG ATCTGTGGCC 
CTGCTACATG CCTTCAAACA GGTTATTCAA GACTATAGCA CACCTCCAAA CGAGGAGTTG 
TCAAGGGACC TTGTGAACAA GCTTTCACCT TATATCAGTT TCCTCAGCCA GTGTCGGCCT 
CTGTCTGCCA GCATGGGCAA TGCCATCAAG TACATAAAGA AGGAGATTTC AAATATACCC 
AATCAATGCA AAGAAGAGGA GGCAAAAGGG AAACTGCAGG CATGTATTGA TAGTTACATA 
AATGAGAAGA TCATTTTGGC TTCTGAAGCC ATTTCTAAAT ATGCTATTGA GAAGATAAGC 
AATGGAGACG TCATTCTAGT TTACGGCTGC TCGTCACTTG TCAATCACAT TCTGTGCGAA 
GCCTTTGAGA AGCAGAGGCA GTTCAGAGTC ATAGTTGTGG ACAGTCGGCC CAGGTTGGAA 
GGTCGAGAGG CGCTGAGGCG TCTGGTGAAG AAAGGCATCC GATGTACTTA CGTGCTCATT 
TCTGCCTTGT CCTACATTCT GCCTGAAGTA TCCAAGGTAT TTCTCGGTGC TCATGCACTC 
CTTGCCAATG GATATGTGAT GTCACGTGTT GGAACTTCCC AAATTGCCCT GGTAGCAAAG 
GCCTACAATG TACCAGTGCT GGTCTGCTGT GAGACCTACA AGTTCTGTGA ACGAGTGCAG 
ACGGACTCTT TTGTATCCAA TGAATTAGAT GACCCTGATG ACCTGATTGT GACCAGAAAT 
GGGAAGACCC ACCTAGAGAA CTGGCAGACT GTTAAATCGC TGGGCCTTCT GAACCTGGTG 
TATGATGTGA CGCCACCAGA CTTTGTGGAC CTTGTGATCA CCGATTTGGG CATGATCCCC 
TGCACCTCTG TGCCTGTGGT ACTGCGAGTC AAGAATGTAG ATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160517.1
CDS	84..1751
Translation	MADSDGRQVDSINHATGEEGKLQDIVKAKSEGKELTKEEKQRLRKEKKQHKKNKKDDNKGPPEKEKEKEKEKASAPPPSKPVTQSPAHKAAAPAPVTASASETPAAAEKPAKTKAELRAERRARQDSERASKQGKKEGGQQSTSSKPKASPNELQPVVKRLPEHVQVDDPAALKKLAKKLERQQKSGQGCGLNITGVQVPLRSDYGYKVSLFSHLHQYSRKNPPTQQISIPATVIHPSIVRLGLQYSQGIIAGSNARSVALLHAFKQVIQDYSTPPNEELSRDLVNKLSPYISFLSQCRPLSASMGNAIKYIKKEISNIPNQCKEEEAKGKLQACIDSYINEKIILASEAISKYAIEKISNGDVILVYGCSSLVNHILCEAFEKQRQFRVIVVDSRPRLEGREALRRLVKKGIRCTYVLISALSYILPEVSKVFLGAHALLANGYVMSRVGTSQIALVAKAYNVPVLVCCETYKFCERVQTDSFVSNELDDPDDLIVTRNGKTHLENWQTVKSLGLLNLVYDVTPPDFVDLVITDLGMIPCTSVPVVLRVKNVDQ

Target ORF information:

RefSeq Version	XM_005160460.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 2B, subunit 4 delta (eif2b4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160460.4

ORF Insert Sequence:

ATGGCGGACT CGGATGGGAG ACAGGTTGAC AGCATCAACC ATGCTACAGG TGAAGAAGGG 
AAGTTGCAAG ATATTGTAAA AGCAAAGAGT GAAGGTAAGG AGTTGACCAA AGAAGAAAAG 
CAGCGTCTTA GGAAAGAGAA GAAGCAGCAT AAAAAGAATA AAAAAGATGA CAACAAGGGT 
CCTCCAGAGA AGGAAAAGGA GAAGGAAAAA GAGAAAGCAT CTGCTCCACC TCCATCAAAG 
CCTGTCACCC AGAGCCCGGC ACATAAAGCA GCTGCTCCAG CACCTGTAAC TGCATCTGCA 
TCTGAAACTC CTGCGGCTGC AGAGAAGCCT GCAAAGACCA AAGCAGAGCT GCGAGCAGAG 
CGACGGGCAC GGCAAGACTC AGAACGAGCA TCAAAACAGG GCAAGAAGGA AGGTGGTCAA 
CAATCCACCT CCAGCAAACC TAAAGCCTCC CCCAATGAGC TCCAGCCAGT TGTGAAAAGG 
TTACCTGAGC ACGTTCAGGT GGATGATCCT GCTGCGTTGA AAAAGTTGGC CAAGAAGCTT 
GAGCGACAGC AGAAGTCAGG GCAGGGATGC GGTTTAAATA TAACTGGAGT CCAGGTTCCT 
TTGAGGTCAG ATTATGGTTA CAAGGTCAGC CTGTTCTCAC ATCTACATCA ATACAGCCGA 
AAAAACCCTC CCACGCAGCA AATCAGCATC CCAGCCACAG TTATTCACCC ATCCATTGTT 
CGGTTGGGCC TGCAGTACTC TCAGGGCATT ATAGCAGGAT CCAATGCTCG ATCTGTGGCC 
CTGCTACATG CCTTCAAACA GGTTATTCAA GACTATAGCA CACCTCCAAA CGAGGAGTTG 
TCAAGGGACC TTGTGAACAA GCTTTCACCT TATATCAGTT TCCTCAGCCA GTGTCGGCCT 
CTGTCTGCCA GCATGGGCAA TGCCATCAAG TACATAAAGA AGGAGATTTC AAATATACCC 
AATCAATGCA AAGAAGAGGA GGCAAAAGGG AAACTGCAGG CATGTATTGA TAGTTACATA 
AATGAGAAGA TCATTTTGGC TTCTGAAGCC ATTTCTAAAT ATGCTATTGA GAAGATAAGC 
AATGGAGACG TCATTCTAGT TTACGGCTGC TCGTCACTTG TCAATCACAT TCTGTGCGAA 
GCCTTTGAGA AGCAGAGGCA GTTCAGAGTC ATAGTTGTGG ACAGTCGGCC CAGGTTGGAA 
GGTCGAGAGG CGCTGAGGCG TCTGGTGAAG AAAGGCATCC GATGTACTTA CGTGCTCATT 
TCTGCCTTGT CCTACATTCT GCCTGAAGTA TCCAAGGTAT TTCTCGGTGC TCATGCACTC 
CTTGCCAATG GATATGTGAT GTCACGTGTT GGAACTTCCC AAATTGCCCT GGTAGCAAAG 
GCCTACAATG TACCAGTGCT GGTCTGCTGT GAGACCTACA AGTTCTGTGA ACGAGTGCAG 
ACGGACTCTT TTGTATCCAA TGAATTAGAT GACCCTGATG ACCTGATTGT GACCAGAAAT 
GGGAAGACCC ACCTAGAGAA CTGGCAGACT GTTAAATCGC TGGGCCTTCT GAACCTGGTG 
TATGATGTGA CGCCACCAGA CTTTGTGGAC CTTGTGATCA CCGATTTGGG CATGATCCCC 
TGCACCTCTG TGCCTGTGGT ACTGCGAGTC AAGAATGTAG ATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2B4 ( XM_005160460.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160517.1 Danio rerio eukaryotic translation initiation factor 2B, subunit 4 delta (eif2b4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24732
Clone ID Related Accession (Same CDS sequence)	XM_005160460.4 , XM_005160460.3
Accession Version	XM_005160460.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	translation initiation factor eIF-2B subunit delta isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160460.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACT CGGATGGGAG ACAGGTTGAC AGCATCAACC ATGCTACAGG TGAAGAAGGG 
AAGTTGCAAG ATATTGTAAA AGCAAAGAGT GAAGGTAAGG AGTTGACCAA AGAAGAAAAG 
CAGCGTCTTA GGAAAGAGAA GAAGCAGCAT AAAAAGAATA AAAAAGATGA CAACAAGGGT 
CCTCCAGAGA AGGAAAAGGA GAAGGAAAAA GAGAAAGCAT CTGCTCCACC TCCATCAAAG 
CCTGTCACCC AGAGCCCGGC ACATAAAGCA GCTGCTCCAG CACCTGTAAC TGCATCTGCA 
TCTGAAACTC CTGCGGCTGC AGAGAAGCCT GCAAAGACCA AAGCAGAGCT GCGAGCAGAG 
CGACGGGCAC GGCAAGACTC AGAACGAGCA TCAAAACAGG GCAAGAAGGA AGGTGGTCAA 
CAATCCACCT CCAGCAAACC TAAAGCCTCC CCCAATGAGC TCCAGCCAGT TGTGAAAAGG 
TTACCTGAGC ACGTTCAGGT GGATGATCCT GCTGCGTTGA AAAAGTTGGC CAAGAAGCTT 
GAGCGACAGC AGAAGTCAGG GCAGGGATGC GGTTTAAATA TAACTGGAGT CCAGGTTCCT 
TTGAGGTCAG ATTATGGTTA CAAGGTCAGC CTGTTCTCAC ATCTACATCA ATACAGCCGA 
AAAAACCCTC CCACGCAGCA AATCAGCATC CCAGCCACAG TTATTCACCC ATCCATTGTT 
CGGTTGGGCC TGCAGTACTC TCAGGGCATT ATAGCAGGAT CCAATGCTCG ATCTGTGGCC 
CTGCTACATG CCTTCAAACA GGTTATTCAA GACTATAGCA CACCTCCAAA CGAGGAGTTG 
TCAAGGGACC TTGTGAACAA GCTTTCACCT TATATCAGTT TCCTCAGCCA GTGTCGGCCT 
CTGTCTGCCA GCATGGGCAA TGCCATCAAG TACATAAAGA AGGAGATTTC AAATATACCC 
AATCAATGCA AAGAAGAGGA GGCAAAAGGG AAACTGCAGG CATGTATTGA TAGTTACATA 
AATGAGAAGA TCATTTTGGC TTCTGAAGCC ATTTCTAAAT ATGCTATTGA GAAGATAAGC 
AATGGAGACG TCATTCTAGT TTACGGCTGC TCGTCACTTG TCAATCACAT TCTGTGCGAA 
GCCTTTGAGA AGCAGAGGCA GTTCAGAGTC ATAGTTGTGG ACAGTCGGCC CAGGTTGGAA 
GGTCGAGAGG CGCTGAGGCG TCTGGTGAAG AAAGGCATCC GATGTACTTA CGTGCTCATT 
TCTGCCTTGT CCTACATTCT GCCTGAAGTA TCCAAGGTAT TTCTCGGTGC TCATGCACTC 
CTTGCCAATG GATATGTGAT GTCACGTGTT GGAACTTCCC AAATTGCCCT GGTAGCAAAG 
GCCTACAATG TACCAGTGCT GGTCTGCTGT GAGACCTACA AGTTCTGTGA ACGAGTGCAG 
ACGGACTCTT TTGTATCCAA TGAATTAGAT GACCCTGATG ACCTGATTGT GACCAGAAAT 
GGGAAGACCC ACCTAGAGAA CTGGCAGACT GTTAAATCGC TGGGCCTTCT GAACCTGGTG 
TATGATGTGA CGCCACCAGA CTTTGTGGAC CTTGTGATCA CCGATTTGGG CATGATCCCC 
TGCACCTCTG TGCCTGTGGT ACTGCGAGTC AAGAATGTAG ATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160517.1
CDS	79..1746
Translation	MADSDGRQVDSINHATGEEGKLQDIVKAKSEGKELTKEEKQRLRKEKKQHKKNKKDDNKGPPEKEKEKEKEKASAPPPSKPVTQSPAHKAAAPAPVTASASETPAAAEKPAKTKAELRAERRARQDSERASKQGKKEGGQQSTSSKPKASPNELQPVVKRLPEHVQVDDPAALKKLAKKLERQQKSGQGCGLNITGVQVPLRSDYGYKVSLFSHLHQYSRKNPPTQQISIPATVIHPSIVRLGLQYSQGIIAGSNARSVALLHAFKQVIQDYSTPPNEELSRDLVNKLSPYISFLSQCRPLSASMGNAIKYIKKEISNIPNQCKEEEAKGKLQACIDSYINEKIILASEAISKYAIEKISNGDVILVYGCSSLVNHILCEAFEKQRQFRVIVVDSRPRLEGREALRRLVKKGIRCTYVLISALSYILPEVSKVFLGAHALLANGYVMSRVGTSQIALVAKAYNVPVLVCCETYKFCERVQTDSFVSNELDDPDDLIVTRNGKTHLENWQTVKSLGLLNLVYDVTPPDFVDLVITDLGMIPCTSVPVVLRVKNVDQ

Target ORF information:

RefSeq Version	XM_005160460.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 2B, subunit 4 delta (eif2b4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160460.3

ORF Insert Sequence:

ATGGCGGACT CGGATGGGAG ACAGGTTGAC AGCATCAACC ATGCTACAGG TGAAGAAGGG 
AAGTTGCAAG ATATTGTAAA AGCAAAGAGT GAAGGTAAGG AGTTGACCAA AGAAGAAAAG 
CAGCGTCTTA GGAAAGAGAA GAAGCAGCAT AAAAAGAATA AAAAAGATGA CAACAAGGGT 
CCTCCAGAGA AGGAAAAGGA GAAGGAAAAA GAGAAAGCAT CTGCTCCACC TCCATCAAAG 
CCTGTCACCC AGAGCCCGGC ACATAAAGCA GCTGCTCCAG CACCTGTAAC TGCATCTGCA 
TCTGAAACTC CTGCGGCTGC AGAGAAGCCT GCAAAGACCA AAGCAGAGCT GCGAGCAGAG 
CGACGGGCAC GGCAAGACTC AGAACGAGCA TCAAAACAGG GCAAGAAGGA AGGTGGTCAA 
CAATCCACCT CCAGCAAACC TAAAGCCTCC CCCAATGAGC TCCAGCCAGT TGTGAAAAGG 
TTACCTGAGC ACGTTCAGGT GGATGATCCT GCTGCGTTGA AAAAGTTGGC CAAGAAGCTT 
GAGCGACAGC AGAAGTCAGG GCAGGGATGC GGTTTAAATA TAACTGGAGT CCAGGTTCCT 
TTGAGGTCAG ATTATGGTTA CAAGGTCAGC CTGTTCTCAC ATCTACATCA ATACAGCCGA 
AAAAACCCTC CCACGCAGCA AATCAGCATC CCAGCCACAG TTATTCACCC ATCCATTGTT 
CGGTTGGGCC TGCAGTACTC TCAGGGCATT ATAGCAGGAT CCAATGCTCG ATCTGTGGCC 
CTGCTACATG CCTTCAAACA GGTTATTCAA GACTATAGCA CACCTCCAAA CGAGGAGTTG 
TCAAGGGACC TTGTGAACAA GCTTTCACCT TATATCAGTT TCCTCAGCCA GTGTCGGCCT 
CTGTCTGCCA GCATGGGCAA TGCCATCAAG TACATAAAGA AGGAGATTTC AAATATACCC 
AATCAATGCA AAGAAGAGGA GGCAAAAGGG AAACTGCAGG CATGTATTGA TAGTTACATA 
AATGAGAAGA TCATTTTGGC TTCTGAAGCC ATTTCTAAAT ATGCTATTGA GAAGATAAGC 
AATGGAGACG TCATTCTAGT TTACGGCTGC TCGTCACTTG TCAATCACAT TCTGTGCGAA 
GCCTTTGAGA AGCAGAGGCA GTTCAGAGTC ATAGTTGTGG ACAGTCGGCC CAGGTTGGAA 
GGTCGAGAGG CGCTGAGGCG TCTGGTGAAG AAAGGCATCC GATGTACTTA CGTGCTCATT 
TCTGCCTTGT CCTACATTCT GCCTGAAGTA TCCAAGGTAT TTCTCGGTGC TCATGCACTC 
CTTGCCAATG GATATGTGAT GTCACGTGTT GGAACTTCCC AAATTGCCCT GGTAGCAAAG 
GCCTACAATG TACCAGTGCT GGTCTGCTGT GAGACCTACA AGTTCTGTGA ACGAGTGCAG 
ACGGACTCTT TTGTATCCAA TGAATTAGAT GACCCTGATG ACCTGATTGT GACCAGAAAT 
GGGAAGACCC ACCTAGAGAA CTGGCAGACT GTTAAATCGC TGGGCCTTCT GAACCTGGTG 
TATGATGTGA CGCCACCAGA CTTTGTGGAC CTTGTGATCA CCGATTTGGG CATGATCCCC 
TGCACCTCTG TGCCTGTGGT ACTGCGAGTC AAGAATGTAG ATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2B4 ( XM_005160461.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160518.1 Danio rerio eukaryotic translation initiation factor 2B, subunit 4 delta (eif2b4), transcript variant X2, mRNA.

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CloneID	ODa24733
Clone ID Related Accession (Same CDS sequence)	XM_005160461.3 , XM_005160461.4
Accession Version	XM_005160461.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	translation initiation factor eIF-2B subunit delta isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160461.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACT CGGATGGGAG ACAGGTTGAC AGCATCAACC ATGCTACAGG TGAAGAAGGG 
AAGTTGCAAG ATATTGTAAA AGCAAAGAGT GAAGGTAAGG AGTTGACCAA AGAAGAAAAG 
CAGCGTCTTA GGAAAGAGAA GAAGCAGCAT AAAAAGAATA AAAAAGATGA CAACAAGGGT 
CCTCCAGAGA AGGAAAAGGA GAAGGAAAAA GAGAAAGCAT CTGCTCCACC TCCATCAAAG 
CCTGTCACCC AGAGCCCGGC ACATAAAGCT GCTCCAGCAC CTGTAACTGC ATCTGCATCT 
GAAACTCCTG CGGCTGCAGA GAAGCCTGCA AAGACCAAAG CAGAGCTGCG AGCAGAGCGA 
CGGGCACGGC AAGACTCAGA ACGAGCATCA AAACAGGGCA AGAAGGAAGG TGGTCAACAA 
TCCACCTCCA GCAAACCTAA AGCCTCCCCC AATGAGCTCC AGCCAGTTGT GAAAAGGTTA 
CCTGAGCACG TTCAGGTGGA TGATCCTGCT GCGTTGAAAA AGTTGGCCAA GAAGCTTGAG 
CGACAGCAGA AGTCAGGGCA GGGATGCGGT TTAAATATAA CTGGAGTCCA GGTTCCTTTG 
AGGTCAGATT ATGGTTACAA GGTCAGCCTG TTCTCACATC TACATCAATA CAGCCGAAAA 
AACCCTCCCA CGCAGCAAAT CAGCATCCCA GCCACAGTTA TTCACCCATC CATTGTTCGG 
TTGGGCCTGC AGTACTCTCA GGGCATTATA GCAGGATCCA ATGCTCGATC TGTGGCCCTG 
CTACATGCCT TCAAACAGGT TATTCAAGAC TATAGCACAC CTCCAAACGA GGAGTTGTCA 
AGGGACCTTG TGAACAAGCT TTCACCTTAT ATCAGTTTCC TCAGCCAGTG TCGGCCTCTG 
TCTGCCAGCA TGGGCAATGC CATCAAGTAC ATAAAGAAGG AGATTTCAAA TATACCCAAT 
CAATGCAAAG AAGAGGAGGC AAAAGGGAAA CTGCAGGCAT GTATTGATAG TTACATAAAT 
GAGAAGATCA TTTTGGCTTC TGAAGCCATT TCTAAATATG CTATTGAGAA GATAAGCAAT 
GGAGACGTCA TTCTAGTTTA CGGCTGCTCG TCACTTGTCA ATCACATTCT GTGCGAAGCC 
TTTGAGAAGC AGAGGCAGTT CAGAGTCATA GTTGTGGACA GTCGGCCCAG GTTGGAAGGT 
CGAGAGGCGC TGAGGCGTCT GGTGAAGAAA GGCATCCGAT GTACTTACGT GCTCATTTCT 
GCCTTGTCCT ACATTCTGCC TGAAGTATCC AAGGTATTTC TCGGTGCTCA TGCACTCCTT 
GCCAATGGAT ATGTGATGTC ACGTGTTGGA ACTTCCCAAA TTGCCCTGGT AGCAAAGGCC 
TACAATGTAC CAGTGCTGGT CTGCTGTGAG ACCTACAAGT TCTGTGAACG AGTGCAGACG 
GACTCTTTTG TATCCAATGA ATTAGATGAC CCTGATGACC TGATTGTGAC CAGAAATGGG 
AAGACCCACC TAGAGAACTG GCAGACTGTT AAATCGCTGG GCCTTCTGAA CCTGGTGTAT 
GATGTGACGC CACCAGACTT TGTGGACCTT GTGATCACCG ATTTGGGCAT GATCCCCTGC 
ACCTCTGTGC CTGTGGTACT GCGAGTCAAG AATGTAGATC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160518.1
CDS	79..1743
Translation	MADSDGRQVDSINHATGEEGKLQDIVKAKSEGKELTKEEKQRLRKEKKQHKKNKKDDNKGPPEKEKEKEKEKASAPPPSKPVTQSPAHKAAPAPVTASASETPAAAEKPAKTKAELRAERRARQDSERASKQGKKEGGQQSTSSKPKASPNELQPVVKRLPEHVQVDDPAALKKLAKKLERQQKSGQGCGLNITGVQVPLRSDYGYKVSLFSHLHQYSRKNPPTQQISIPATVIHPSIVRLGLQYSQGIIAGSNARSVALLHAFKQVIQDYSTPPNEELSRDLVNKLSPYISFLSQCRPLSASMGNAIKYIKKEISNIPNQCKEEEAKGKLQACIDSYINEKIILASEAISKYAIEKISNGDVILVYGCSSLVNHILCEAFEKQRQFRVIVVDSRPRLEGREALRRLVKKGIRCTYVLISALSYILPEVSKVFLGAHALLANGYVMSRVGTSQIALVAKAYNVPVLVCCETYKFCERVQTDSFVSNELDDPDDLIVTRNGKTHLENWQTVKSLGLLNLVYDVTPPDFVDLVITDLGMIPCTSVPVVLRVKNVDQ

Target ORF information:

RefSeq Version	XM_005160461.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 2B, subunit 4 delta (eif2b4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160461.3

ORF Insert Sequence:

ATGGCGGACT CGGATGGGAG ACAGGTTGAC AGCATCAACC ATGCTACAGG TGAAGAAGGG 
AAGTTGCAAG ATATTGTAAA AGCAAAGAGT GAAGGTAAGG AGTTGACCAA AGAAGAAAAG 
CAGCGTCTTA GGAAAGAGAA GAAGCAGCAT AAAAAGAATA AAAAAGATGA CAACAAGGGT 
CCTCCAGAGA AGGAAAAGGA GAAGGAAAAA GAGAAAGCAT CTGCTCCACC TCCATCAAAG 
CCTGTCACCC AGAGCCCGGC ACATAAAGCT GCTCCAGCAC CTGTAACTGC ATCTGCATCT 
GAAACTCCTG CGGCTGCAGA GAAGCCTGCA AAGACCAAAG CAGAGCTGCG AGCAGAGCGA 
CGGGCACGGC AAGACTCAGA ACGAGCATCA AAACAGGGCA AGAAGGAAGG TGGTCAACAA 
TCCACCTCCA GCAAACCTAA AGCCTCCCCC AATGAGCTCC AGCCAGTTGT GAAAAGGTTA 
CCTGAGCACG TTCAGGTGGA TGATCCTGCT GCGTTGAAAA AGTTGGCCAA GAAGCTTGAG 
CGACAGCAGA AGTCAGGGCA GGGATGCGGT TTAAATATAA CTGGAGTCCA GGTTCCTTTG 
AGGTCAGATT ATGGTTACAA GGTCAGCCTG TTCTCACATC TACATCAATA CAGCCGAAAA 
AACCCTCCCA CGCAGCAAAT CAGCATCCCA GCCACAGTTA TTCACCCATC CATTGTTCGG 
TTGGGCCTGC AGTACTCTCA GGGCATTATA GCAGGATCCA ATGCTCGATC TGTGGCCCTG 
CTACATGCCT TCAAACAGGT TATTCAAGAC TATAGCACAC CTCCAAACGA GGAGTTGTCA 
AGGGACCTTG TGAACAAGCT TTCACCTTAT ATCAGTTTCC TCAGCCAGTG TCGGCCTCTG 
TCTGCCAGCA TGGGCAATGC CATCAAGTAC ATAAAGAAGG AGATTTCAAA TATACCCAAT 
CAATGCAAAG AAGAGGAGGC AAAAGGGAAA CTGCAGGCAT GTATTGATAG TTACATAAAT 
GAGAAGATCA TTTTGGCTTC TGAAGCCATT TCTAAATATG CTATTGAGAA GATAAGCAAT 
GGAGACGTCA TTCTAGTTTA CGGCTGCTCG TCACTTGTCA ATCACATTCT GTGCGAAGCC 
TTTGAGAAGC AGAGGCAGTT CAGAGTCATA GTTGTGGACA GTCGGCCCAG GTTGGAAGGT 
CGAGAGGCGC TGAGGCGTCT GGTGAAGAAA GGCATCCGAT GTACTTACGT GCTCATTTCT 
GCCTTGTCCT ACATTCTGCC TGAAGTATCC AAGGTATTTC TCGGTGCTCA TGCACTCCTT 
GCCAATGGAT ATGTGATGTC ACGTGTTGGA ACTTCCCAAA TTGCCCTGGT AGCAAAGGCC 
TACAATGTAC CAGTGCTGGT CTGCTGTGAG ACCTACAAGT TCTGTGAACG AGTGCAGACG 
GACTCTTTTG TATCCAATGA ATTAGATGAC CCTGATGACC TGATTGTGAC CAGAAATGGG 
AAGACCCACC TAGAGAACTG GCAGACTGTT AAATCGCTGG GCCTTCTGAA CCTGGTGTAT 
GATGTGACGC CACCAGACTT TGTGGACCTT GTGATCACCG ATTTGGGCAT GATCCCCTGC 
ACCTCTGTGC CTGTGGTACT GCGAGTCAAG AATGTAGATC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2B4 ( XM_005160461.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160518.1 Danio rerio eukaryotic translation initiation factor 2B, subunit 4 delta (eif2b4), transcript variant X2, mRNA.

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CloneID	ODa24733
Clone ID Related Accession (Same CDS sequence)	XM_005160461.3 , XM_005160461.4
Accession Version	XM_005160461.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	translation initiation factor eIF-2B subunit delta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160461.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACT CGGATGGGAG ACAGGTTGAC AGCATCAACC ATGCTACAGG TGAAGAAGGG 
AAGTTGCAAG ATATTGTAAA AGCAAAGAGT GAAGGTAAGG AGTTGACCAA AGAAGAAAAG 
CAGCGTCTTA GGAAAGAGAA GAAGCAGCAT AAAAAGAATA AAAAAGATGA CAACAAGGGT 
CCTCCAGAGA AGGAAAAGGA GAAGGAAAAA GAGAAAGCAT CTGCTCCACC TCCATCAAAG 
CCTGTCACCC AGAGCCCGGC ACATAAAGCT GCTCCAGCAC CTGTAACTGC ATCTGCATCT 
GAAACTCCTG CGGCTGCAGA GAAGCCTGCA AAGACCAAAG CAGAGCTGCG AGCAGAGCGA 
CGGGCACGGC AAGACTCAGA ACGAGCATCA AAACAGGGCA AGAAGGAAGG TGGTCAACAA 
TCCACCTCCA GCAAACCTAA AGCCTCCCCC AATGAGCTCC AGCCAGTTGT GAAAAGGTTA 
CCTGAGCACG TTCAGGTGGA TGATCCTGCT GCGTTGAAAA AGTTGGCCAA GAAGCTTGAG 
CGACAGCAGA AGTCAGGGCA GGGATGCGGT TTAAATATAA CTGGAGTCCA GGTTCCTTTG 
AGGTCAGATT ATGGTTACAA GGTCAGCCTG TTCTCACATC TACATCAATA CAGCCGAAAA 
AACCCTCCCA CGCAGCAAAT CAGCATCCCA GCCACAGTTA TTCACCCATC CATTGTTCGG 
TTGGGCCTGC AGTACTCTCA GGGCATTATA GCAGGATCCA ATGCTCGATC TGTGGCCCTG 
CTACATGCCT TCAAACAGGT TATTCAAGAC TATAGCACAC CTCCAAACGA GGAGTTGTCA 
AGGGACCTTG TGAACAAGCT TTCACCTTAT ATCAGTTTCC TCAGCCAGTG TCGGCCTCTG 
TCTGCCAGCA TGGGCAATGC CATCAAGTAC ATAAAGAAGG AGATTTCAAA TATACCCAAT 
CAATGCAAAG AAGAGGAGGC AAAAGGGAAA CTGCAGGCAT GTATTGATAG TTACATAAAT 
GAGAAGATCA TTTTGGCTTC TGAAGCCATT TCTAAATATG CTATTGAGAA GATAAGCAAT 
GGAGACGTCA TTCTAGTTTA CGGCTGCTCG TCACTTGTCA ATCACATTCT GTGCGAAGCC 
TTTGAGAAGC AGAGGCAGTT CAGAGTCATA GTTGTGGACA GTCGGCCCAG GTTGGAAGGT 
CGAGAGGCGC TGAGGCGTCT GGTGAAGAAA GGCATCCGAT GTACTTACGT GCTCATTTCT 
GCCTTGTCCT ACATTCTGCC TGAAGTATCC AAGGTATTTC TCGGTGCTCA TGCACTCCTT 
GCCAATGGAT ATGTGATGTC ACGTGTTGGA ACTTCCCAAA TTGCCCTGGT AGCAAAGGCC 
TACAATGTAC CAGTGCTGGT CTGCTGTGAG ACCTACAAGT TCTGTGAACG AGTGCAGACG 
GACTCTTTTG TATCCAATGA ATTAGATGAC CCTGATGACC TGATTGTGAC CAGAAATGGG 
AAGACCCACC TAGAGAACTG GCAGACTGTT AAATCGCTGG GCCTTCTGAA CCTGGTGTAT 
GATGTGACGC CACCAGACTT TGTGGACCTT GTGATCACCG ATTTGGGCAT GATCCCCTGC 
ACCTCTGTGC CTGTGGTACT GCGAGTCAAG AATGTAGATC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160518.1
CDS	84..1748
Translation	MADSDGRQVDSINHATGEEGKLQDIVKAKSEGKELTKEEKQRLRKEKKQHKKNKKDDNKGPPEKEKEKEKEKASAPPPSKPVTQSPAHKAAPAPVTASASETPAAAEKPAKTKAELRAERRARQDSERASKQGKKEGGQQSTSSKPKASPNELQPVVKRLPEHVQVDDPAALKKLAKKLERQQKSGQGCGLNITGVQVPLRSDYGYKVSLFSHLHQYSRKNPPTQQISIPATVIHPSIVRLGLQYSQGIIAGSNARSVALLHAFKQVIQDYSTPPNEELSRDLVNKLSPYISFLSQCRPLSASMGNAIKYIKKEISNIPNQCKEEEAKGKLQACIDSYINEKIILASEAISKYAIEKISNGDVILVYGCSSLVNHILCEAFEKQRQFRVIVVDSRPRLEGREALRRLVKKGIRCTYVLISALSYILPEVSKVFLGAHALLANGYVMSRVGTSQIALVAKAYNVPVLVCCETYKFCERVQTDSFVSNELDDPDDLIVTRNGKTHLENWQTVKSLGLLNLVYDVTPPDFVDLVITDLGMIPCTSVPVVLRVKNVDQ

Target ORF information:

RefSeq Version	XM_005160461.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 2B, subunit 4 delta (eif2b4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160461.4

ORF Insert Sequence:

ATGGCGGACT CGGATGGGAG ACAGGTTGAC AGCATCAACC ATGCTACAGG TGAAGAAGGG 
AAGTTGCAAG ATATTGTAAA AGCAAAGAGT GAAGGTAAGG AGTTGACCAA AGAAGAAAAG 
CAGCGTCTTA GGAAAGAGAA GAAGCAGCAT AAAAAGAATA AAAAAGATGA CAACAAGGGT 
CCTCCAGAGA AGGAAAAGGA GAAGGAAAAA GAGAAAGCAT CTGCTCCACC TCCATCAAAG 
CCTGTCACCC AGAGCCCGGC ACATAAAGCT GCTCCAGCAC CTGTAACTGC ATCTGCATCT 
GAAACTCCTG CGGCTGCAGA GAAGCCTGCA AAGACCAAAG CAGAGCTGCG AGCAGAGCGA 
CGGGCACGGC AAGACTCAGA ACGAGCATCA AAACAGGGCA AGAAGGAAGG TGGTCAACAA 
TCCACCTCCA GCAAACCTAA AGCCTCCCCC AATGAGCTCC AGCCAGTTGT GAAAAGGTTA 
CCTGAGCACG TTCAGGTGGA TGATCCTGCT GCGTTGAAAA AGTTGGCCAA GAAGCTTGAG 
CGACAGCAGA AGTCAGGGCA GGGATGCGGT TTAAATATAA CTGGAGTCCA GGTTCCTTTG 
AGGTCAGATT ATGGTTACAA GGTCAGCCTG TTCTCACATC TACATCAATA CAGCCGAAAA 
AACCCTCCCA CGCAGCAAAT CAGCATCCCA GCCACAGTTA TTCACCCATC CATTGTTCGG 
TTGGGCCTGC AGTACTCTCA GGGCATTATA GCAGGATCCA ATGCTCGATC TGTGGCCCTG 
CTACATGCCT TCAAACAGGT TATTCAAGAC TATAGCACAC CTCCAAACGA GGAGTTGTCA 
AGGGACCTTG TGAACAAGCT TTCACCTTAT ATCAGTTTCC TCAGCCAGTG TCGGCCTCTG 
TCTGCCAGCA TGGGCAATGC CATCAAGTAC ATAAAGAAGG AGATTTCAAA TATACCCAAT 
CAATGCAAAG AAGAGGAGGC AAAAGGGAAA CTGCAGGCAT GTATTGATAG TTACATAAAT 
GAGAAGATCA TTTTGGCTTC TGAAGCCATT TCTAAATATG CTATTGAGAA GATAAGCAAT 
GGAGACGTCA TTCTAGTTTA CGGCTGCTCG TCACTTGTCA ATCACATTCT GTGCGAAGCC 
TTTGAGAAGC AGAGGCAGTT CAGAGTCATA GTTGTGGACA GTCGGCCCAG GTTGGAAGGT 
CGAGAGGCGC TGAGGCGTCT GGTGAAGAAA GGCATCCGAT GTACTTACGT GCTCATTTCT 
GCCTTGTCCT ACATTCTGCC TGAAGTATCC AAGGTATTTC TCGGTGCTCA TGCACTCCTT 
GCCAATGGAT ATGTGATGTC ACGTGTTGGA ACTTCCCAAA TTGCCCTGGT AGCAAAGGCC 
TACAATGTAC CAGTGCTGGT CTGCTGTGAG ACCTACAAGT TCTGTGAACG AGTGCAGACG 
GACTCTTTTG TATCCAATGA ATTAGATGAC CCTGATGACC TGATTGTGAC CAGAAATGGG 
AAGACCCACC TAGAGAACTG GCAGACTGTT AAATCGCTGG GCCTTCTGAA CCTGGTGTAT 
GATGTGACGC CACCAGACTT TGTGGACCTT GTGATCACCG ATTTGGGCAT GATCCCCTGC 
ACCTCTGTGC CTGTGGTACT GCGAGTCAAG AATGTAGATC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2B4 ( XM_005160462.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160519.1 Danio rerio eukaryotic translation initiation factor 2B, subunit 4 delta (eif2b4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa24734
Clone ID Related Accession (Same CDS sequence)	XM_005160462.4 , XM_005160462.3
Accession Version	XM_005160462.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	translation initiation factor eIF-2B subunit delta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160462.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACT CGGATGGGAG ACAGGTTGAC AGCATCAACC ATGCTACAGG TGAAGAAGGG 
AAGTTGCAAG ATATTGTAAA AGCAAAGAGT GAAGGTAAGG AGTTGACCAA AGAAGAAAAG 
CAGCGTCTTA GGAAAGAGAA GAAGCAGCAT AAAAAGAATA AAAAAGATGA CAACAAGGGT 
CCTCCAGAGA AGGAAAAGGA GAAGGAAAAA GAGAAAGCAT CTGCTCCACC TCCATCAAAG 
CCTGTCACCC AGAGCCCGGC ACATAAAGCA GCTGCTCCAG CACCTGTAAC TGCATCTGCA 
TCTGAAACTC CTGCGGCTGC AGAGAAGCCT GCAAAGACCA AAGCAGAGCT GCGAGCAGAG 
CGACGGGCAC GGCAAGACTC AGAACGAGCA TCAAAACAGG GCAAGAAGGA AGGTGGTCAA 
CAATCCACCT CCAGCAAACC TAAAGCCTCC CCCAATGAGC TCCAGCCAGT TGTGAAAAGG 
TTACCTGAGC ACGTTCAGGT GGATGATCCT GCTGCGTTGA AAAAGTTGGC CAAGAAGCTT 
GAGCGACAGC AGGTTCCTTT GAGGTCAGAT TATGGTTACA AGGTCAGCCT GTTCTCACAT 
CTACATCAAT ACAGCCGAAA AAACCCTCCC ACGCAGCAAA TCAGCATCCC AGCCACAGTT 
ATTCACCCAT CCATTGTTCG GTTGGGCCTG CAGTACTCTC AGGGCATTAT AGCAGGATCC 
AATGCTCGAT CTGTGGCCCT GCTACATGCC TTCAAACAGG TTATTCAAGA CTATAGCACA 
CCTCCAAACG AGGAGTTGTC AAGGGACCTT GTGAACAAGC TTTCACCTTA TATCAGTTTC 
CTCAGCCAGT GTCGGCCTCT GTCTGCCAGC ATGGGCAATG CCATCAAGTA CATAAAGAAG 
GAGATTTCAA ATATACCCAA TCAATGCAAA GAAGAGGAGG CAAAAGGGAA ACTGCAGGCA 
TGTATTGATA GTTACATAAA TGAGAAGATC ATTTTGGCTT CTGAAGCCAT TTCTAAATAT 
GCTATTGAGA AGATAAGCAA TGGAGACGTC ATTCTAGTTT ACGGCTGCTC GTCACTTGTC 
AATCACATTC TGTGCGAAGC CTTTGAGAAG CAGAGGCAGT TCAGAGTCAT AGTTGTGGAC 
AGTCGGCCCA GGTTGGAAGG TCGAGAGGCG CTGAGGCGTC TGGTGAAGAA AGGCATCCGA 
TGTACTTACG TGCTCATTTC TGCCTTGTCC TACATTCTGC CTGAAGTATC CAAGGTATTT 
CTCGGTGCTC ATGCACTCCT TGCCAATGGA TATGTGATGT CACGTGTTGG AACTTCCCAA 
ATTGCCCTGG TAGCAAAGGC CTACAATGTA CCAGTGCTGG TCTGCTGTGA GACCTACAAG 
TTCTGTGAAC GAGTGCAGAC GGACTCTTTT GTATCCAATG AATTAGATGA CCCTGATGAC 
CTGATTGTGA CCAGAAATGG GAAGACCCAC CTAGAGAACT GGCAGACTGT TAAATCGCTG 
GGCCTTCTGA ACCTGGTGTA TGATGTGACG CCACCAGACT TTGTGGACCT TGTGATCACC 
GATTTGGGCA TGATCCCCTG CACCTCTGTG CCTGTGGTAC TGCGAGTCAA GAATGTAGAT 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160519.1
CDS	83..1708
Translation	MADSDGRQVDSINHATGEEGKLQDIVKAKSEGKELTKEEKQRLRKEKKQHKKNKKDDNKGPPEKEKEKEKEKASAPPPSKPVTQSPAHKAAAPAPVTASASETPAAAEKPAKTKAELRAERRARQDSERASKQGKKEGGQQSTSSKPKASPNELQPVVKRLPEHVQVDDPAALKKLAKKLERQQVPLRSDYGYKVSLFSHLHQYSRKNPPTQQISIPATVIHPSIVRLGLQYSQGIIAGSNARSVALLHAFKQVIQDYSTPPNEELSRDLVNKLSPYISFLSQCRPLSASMGNAIKYIKKEISNIPNQCKEEEAKGKLQACIDSYINEKIILASEAISKYAIEKISNGDVILVYGCSSLVNHILCEAFEKQRQFRVIVVDSRPRLEGREALRRLVKKGIRCTYVLISALSYILPEVSKVFLGAHALLANGYVMSRVGTSQIALVAKAYNVPVLVCCETYKFCERVQTDSFVSNELDDPDDLIVTRNGKTHLENWQTVKSLGLLNLVYDVTPPDFVDLVITDLGMIPCTSVPVVLRVKNVDQ

Target ORF information:

RefSeq Version	XM_005160462.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 2B, subunit 4 delta (eif2b4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160462.4

ORF Insert Sequence:

ATGGCGGACT CGGATGGGAG ACAGGTTGAC AGCATCAACC ATGCTACAGG TGAAGAAGGG 
AAGTTGCAAG ATATTGTAAA AGCAAAGAGT GAAGGTAAGG AGTTGACCAA AGAAGAAAAG 
CAGCGTCTTA GGAAAGAGAA GAAGCAGCAT AAAAAGAATA AAAAAGATGA CAACAAGGGT 
CCTCCAGAGA AGGAAAAGGA GAAGGAAAAA GAGAAAGCAT CTGCTCCACC TCCATCAAAG 
CCTGTCACCC AGAGCCCGGC ACATAAAGCA GCTGCTCCAG CACCTGTAAC TGCATCTGCA 
TCTGAAACTC CTGCGGCTGC AGAGAAGCCT GCAAAGACCA AAGCAGAGCT GCGAGCAGAG 
CGACGGGCAC GGCAAGACTC AGAACGAGCA TCAAAACAGG GCAAGAAGGA AGGTGGTCAA 
CAATCCACCT CCAGCAAACC TAAAGCCTCC CCCAATGAGC TCCAGCCAGT TGTGAAAAGG 
TTACCTGAGC ACGTTCAGGT GGATGATCCT GCTGCGTTGA AAAAGTTGGC CAAGAAGCTT 
GAGCGACAGC AGGTTCCTTT GAGGTCAGAT TATGGTTACA AGGTCAGCCT GTTCTCACAT 
CTACATCAAT ACAGCCGAAA AAACCCTCCC ACGCAGCAAA TCAGCATCCC AGCCACAGTT 
ATTCACCCAT CCATTGTTCG GTTGGGCCTG CAGTACTCTC AGGGCATTAT AGCAGGATCC 
AATGCTCGAT CTGTGGCCCT GCTACATGCC TTCAAACAGG TTATTCAAGA CTATAGCACA 
CCTCCAAACG AGGAGTTGTC AAGGGACCTT GTGAACAAGC TTTCACCTTA TATCAGTTTC 
CTCAGCCAGT GTCGGCCTCT GTCTGCCAGC ATGGGCAATG CCATCAAGTA CATAAAGAAG 
GAGATTTCAA ATATACCCAA TCAATGCAAA GAAGAGGAGG CAAAAGGGAA ACTGCAGGCA 
TGTATTGATA GTTACATAAA TGAGAAGATC ATTTTGGCTT CTGAAGCCAT TTCTAAATAT 
GCTATTGAGA AGATAAGCAA TGGAGACGTC ATTCTAGTTT ACGGCTGCTC GTCACTTGTC 
AATCACATTC TGTGCGAAGC CTTTGAGAAG CAGAGGCAGT TCAGAGTCAT AGTTGTGGAC 
AGTCGGCCCA GGTTGGAAGG TCGAGAGGCG CTGAGGCGTC TGGTGAAGAA AGGCATCCGA 
TGTACTTACG TGCTCATTTC TGCCTTGTCC TACATTCTGC CTGAAGTATC CAAGGTATTT 
CTCGGTGCTC ATGCACTCCT TGCCAATGGA TATGTGATGT CACGTGTTGG AACTTCCCAA 
ATTGCCCTGG TAGCAAAGGC CTACAATGTA CCAGTGCTGG TCTGCTGTGA GACCTACAAG 
TTCTGTGAAC GAGTGCAGAC GGACTCTTTT GTATCCAATG AATTAGATGA CCCTGATGAC 
CTGATTGTGA CCAGAAATGG GAAGACCCAC CTAGAGAACT GGCAGACTGT TAAATCGCTG 
GGCCTTCTGA ACCTGGTGTA TGATGTGACG CCACCAGACT TTGTGGACCT TGTGATCACC 
GATTTGGGCA TGATCCCCTG CACCTCTGTG CCTGTGGTAC TGCGAGTCAA GAATGTAGAT 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2B4 ( XM_005160462.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160519.1 Danio rerio eukaryotic translation initiation factor 2B, subunit 4 delta (eif2b4), transcript variant X3, mRNA.

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CloneID	ODa24734
Clone ID Related Accession (Same CDS sequence)	XM_005160462.4 , XM_005160462.3
Accession Version	XM_005160462.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	translation initiation factor eIF-2B subunit delta isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160462.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACT CGGATGGGAG ACAGGTTGAC AGCATCAACC ATGCTACAGG TGAAGAAGGG 
AAGTTGCAAG ATATTGTAAA AGCAAAGAGT GAAGGTAAGG AGTTGACCAA AGAAGAAAAG 
CAGCGTCTTA GGAAAGAGAA GAAGCAGCAT AAAAAGAATA AAAAAGATGA CAACAAGGGT 
CCTCCAGAGA AGGAAAAGGA GAAGGAAAAA GAGAAAGCAT CTGCTCCACC TCCATCAAAG 
CCTGTCACCC AGAGCCCGGC ACATAAAGCA GCTGCTCCAG CACCTGTAAC TGCATCTGCA 
TCTGAAACTC CTGCGGCTGC AGAGAAGCCT GCAAAGACCA AAGCAGAGCT GCGAGCAGAG 
CGACGGGCAC GGCAAGACTC AGAACGAGCA TCAAAACAGG GCAAGAAGGA AGGTGGTCAA 
CAATCCACCT CCAGCAAACC TAAAGCCTCC CCCAATGAGC TCCAGCCAGT TGTGAAAAGG 
TTACCTGAGC ACGTTCAGGT GGATGATCCT GCTGCGTTGA AAAAGTTGGC CAAGAAGCTT 
GAGCGACAGC AGGTTCCTTT GAGGTCAGAT TATGGTTACA AGGTCAGCCT GTTCTCACAT 
CTACATCAAT ACAGCCGAAA AAACCCTCCC ACGCAGCAAA TCAGCATCCC AGCCACAGTT 
ATTCACCCAT CCATTGTTCG GTTGGGCCTG CAGTACTCTC AGGGCATTAT AGCAGGATCC 
AATGCTCGAT CTGTGGCCCT GCTACATGCC TTCAAACAGG TTATTCAAGA CTATAGCACA 
CCTCCAAACG AGGAGTTGTC AAGGGACCTT GTGAACAAGC TTTCACCTTA TATCAGTTTC 
CTCAGCCAGT GTCGGCCTCT GTCTGCCAGC ATGGGCAATG CCATCAAGTA CATAAAGAAG 
GAGATTTCAA ATATACCCAA TCAATGCAAA GAAGAGGAGG CAAAAGGGAA ACTGCAGGCA 
TGTATTGATA GTTACATAAA TGAGAAGATC ATTTTGGCTT CTGAAGCCAT TTCTAAATAT 
GCTATTGAGA AGATAAGCAA TGGAGACGTC ATTCTAGTTT ACGGCTGCTC GTCACTTGTC 
AATCACATTC TGTGCGAAGC CTTTGAGAAG CAGAGGCAGT TCAGAGTCAT AGTTGTGGAC 
AGTCGGCCCA GGTTGGAAGG TCGAGAGGCG CTGAGGCGTC TGGTGAAGAA AGGCATCCGA 
TGTACTTACG TGCTCATTTC TGCCTTGTCC TACATTCTGC CTGAAGTATC CAAGGTATTT 
CTCGGTGCTC ATGCACTCCT TGCCAATGGA TATGTGATGT CACGTGTTGG AACTTCCCAA 
ATTGCCCTGG TAGCAAAGGC CTACAATGTA CCAGTGCTGG TCTGCTGTGA GACCTACAAG 
TTCTGTGAAC GAGTGCAGAC GGACTCTTTT GTATCCAATG AATTAGATGA CCCTGATGAC 
CTGATTGTGA CCAGAAATGG GAAGACCCAC CTAGAGAACT GGCAGACTGT TAAATCGCTG 
GGCCTTCTGA ACCTGGTGTA TGATGTGACG CCACCAGACT TTGTGGACCT TGTGATCACC 
GATTTGGGCA TGATCCCCTG CACCTCTGTG CCTGTGGTAC TGCGAGTCAA GAATGTAGAT 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160519.1
CDS	79..1704
Translation	MADSDGRQVDSINHATGEEGKLQDIVKAKSEGKELTKEEKQRLRKEKKQHKKNKKDDNKGPPEKEKEKEKEKASAPPPSKPVTQSPAHKAAAPAPVTASASETPAAAEKPAKTKAELRAERRARQDSERASKQGKKEGGQQSTSSKPKASPNELQPVVKRLPEHVQVDDPAALKKLAKKLERQQVPLRSDYGYKVSLFSHLHQYSRKNPPTQQISIPATVIHPSIVRLGLQYSQGIIAGSNARSVALLHAFKQVIQDYSTPPNEELSRDLVNKLSPYISFLSQCRPLSASMGNAIKYIKKEISNIPNQCKEEEAKGKLQACIDSYINEKIILASEAISKYAIEKISNGDVILVYGCSSLVNHILCEAFEKQRQFRVIVVDSRPRLEGREALRRLVKKGIRCTYVLISALSYILPEVSKVFLGAHALLANGYVMSRVGTSQIALVAKAYNVPVLVCCETYKFCERVQTDSFVSNELDDPDDLIVTRNGKTHLENWQTVKSLGLLNLVYDVTPPDFVDLVITDLGMIPCTSVPVVLRVKNVDQ

Target ORF information:

RefSeq Version	XM_005160462.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 2B, subunit 4 delta (eif2b4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160462.3

ORF Insert Sequence:

ATGGCGGACT CGGATGGGAG ACAGGTTGAC AGCATCAACC ATGCTACAGG TGAAGAAGGG 
AAGTTGCAAG ATATTGTAAA AGCAAAGAGT GAAGGTAAGG AGTTGACCAA AGAAGAAAAG 
CAGCGTCTTA GGAAAGAGAA GAAGCAGCAT AAAAAGAATA AAAAAGATGA CAACAAGGGT 
CCTCCAGAGA AGGAAAAGGA GAAGGAAAAA GAGAAAGCAT CTGCTCCACC TCCATCAAAG 
CCTGTCACCC AGAGCCCGGC ACATAAAGCA GCTGCTCCAG CACCTGTAAC TGCATCTGCA 
TCTGAAACTC CTGCGGCTGC AGAGAAGCCT GCAAAGACCA AAGCAGAGCT GCGAGCAGAG 
CGACGGGCAC GGCAAGACTC AGAACGAGCA TCAAAACAGG GCAAGAAGGA AGGTGGTCAA 
CAATCCACCT CCAGCAAACC TAAAGCCTCC CCCAATGAGC TCCAGCCAGT TGTGAAAAGG 
TTACCTGAGC ACGTTCAGGT GGATGATCCT GCTGCGTTGA AAAAGTTGGC CAAGAAGCTT 
GAGCGACAGC AGGTTCCTTT GAGGTCAGAT TATGGTTACA AGGTCAGCCT GTTCTCACAT 
CTACATCAAT ACAGCCGAAA AAACCCTCCC ACGCAGCAAA TCAGCATCCC AGCCACAGTT 
ATTCACCCAT CCATTGTTCG GTTGGGCCTG CAGTACTCTC AGGGCATTAT AGCAGGATCC 
AATGCTCGAT CTGTGGCCCT GCTACATGCC TTCAAACAGG TTATTCAAGA CTATAGCACA 
CCTCCAAACG AGGAGTTGTC AAGGGACCTT GTGAACAAGC TTTCACCTTA TATCAGTTTC 
CTCAGCCAGT GTCGGCCTCT GTCTGCCAGC ATGGGCAATG CCATCAAGTA CATAAAGAAG 
GAGATTTCAA ATATACCCAA TCAATGCAAA GAAGAGGAGG CAAAAGGGAA ACTGCAGGCA 
TGTATTGATA GTTACATAAA TGAGAAGATC ATTTTGGCTT CTGAAGCCAT TTCTAAATAT 
GCTATTGAGA AGATAAGCAA TGGAGACGTC ATTCTAGTTT ACGGCTGCTC GTCACTTGTC 
AATCACATTC TGTGCGAAGC CTTTGAGAAG CAGAGGCAGT TCAGAGTCAT AGTTGTGGAC 
AGTCGGCCCA GGTTGGAAGG TCGAGAGGCG CTGAGGCGTC TGGTGAAGAA AGGCATCCGA 
TGTACTTACG TGCTCATTTC TGCCTTGTCC TACATTCTGC CTGAAGTATC CAAGGTATTT 
CTCGGTGCTC ATGCACTCCT TGCCAATGGA TATGTGATGT CACGTGTTGG AACTTCCCAA 
ATTGCCCTGG TAGCAAAGGC CTACAATGTA CCAGTGCTGG TCTGCTGTGA GACCTACAAG 
TTCTGTGAAC GAGTGCAGAC GGACTCTTTT GTATCCAATG AATTAGATGA CCCTGATGAC 
CTGATTGTGA CCAGAAATGG GAAGACCCAC CTAGAGAACT GGCAGACTGT TAAATCGCTG 
GGCCTTCTGA ACCTGGTGTA TGATGTGACG CCACCAGACT TTGTGGACCT TGTGATCACC 
GATTTGGGCA TGATCCCCTG CACCTCTGTG CCTGTGGTAC TGCGAGTCAA GAATGTAGAT 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Evolution and genomic organization of muscle microRNAs in fish genomes.
BMC evolutionary biology14196(2014 Sep)
Nachtigall PG,Dias MC,Pinhal D

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

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EIF2B4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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