trdmt1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	trdmt1
Entrez Gene ID	449878
Full Name	tRNA aspartic acid methyltransferase 1
Synonyms	dnmt2,im:7138158,zgc:136961
General protein information	Preferred Names tRNA aspartic acid methyltransferase 1 Names tRNA (cytosine(38)-C(5))-methyltransferase DNA (cytosine-5-)-methyltransferase 2 DNA methyltransferase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001018143.1	NP_001018153.1	tRNA (cytosine(38)-C(5))-methyltransferase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TRDMT1	XP_848593.1
Pan troglodytes (chimpanzee)	TRDMT1	XP_001151840.2
Rattus norvegicus (Norway rat)	Trdmt1	NP_001026813.1
Gallus gallus (chicken)	TRDMT1	NP_001020002.1
Bos taurus (cattle)	TRDMT1	NP_861528.1
Mus musculus (house mouse)	Trdmt1	NP_034197.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004101	XP_312975.5
Homo sapiens (human)	TRDMT1	NP_004403.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012836	XP_306830.2
Arabidopsis thaliana (thale cress)	DNMT2	NP_568474.1
Xenopus tropicalis (tropical clawed frog)	trdmt1	NP_001004959.2
Danio rerio (zebrafish)	trdmt1	NP_001018153.1
Drosophila melanogaster (fruit fly)	Mt2	NP_001036355.1
Schizosaccharomyces pombe (fission yeast)	pmt1	NP_595687.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Partial depletion of yolk during zebrafish embryogenesis changes the dynamics of methionine cycle and metabolic genes.
Huang Y, Linsen SE
BMC genomics16427(2015 Jun)

Expression of the de novo DNA methyltransferases (dnmt3 - dnmt8) during zebrafish lens development.
Seritrakul P, Gross JM
Developmental dynamics : an official publication of the American Association of Anatomists243(2)350-6(2014 Feb)

Gonad differentiation in zebrafish is regulated by the canonical Wnt signaling pathway.
Sreenivasan R, Jiang J, Wang X, B?rtfai R, Kwan HY, Christoffels A, Orb?n L
Biology of reproduction90(2)45(2014 Feb)

Analysis of DNA methylation reveals a partial reprogramming of the M?ller glia genome during retina regeneration.
Powell C, Grant AR, Cornblath E, Goldman D
Proceedings of the National Academy of Sciences of the United States of America110(49)19814-9(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dnmt2 promotes zebrafish development, likely through cytoplasmic RNA methylation.
Title: Dnmt2 functions in the cytoplasm to promote liver, brain, and retina development in zebrafish.

The following trdmt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trdmt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01158	NM_001018143.1 Latest version!	Danio rerio tRNA aspartic acid methyltransferase 1 (trdmt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trdmt1 ( NM_001018143.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001018153.1 Danio rerio tRNA aspartic acid methyltransferase 1 (trdmt1), mRNA.

Price & Availability↑

CloneID	ODa01158
Clone ID Related Accession (Same CDS sequence)	NM_001018143.1
Accession Version	NM_001018143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	tRNA (cytosine(38)-C(5))-methyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB196920.1. ##Evidence-Data-START## Transcript exon combination :: AB196920.1, BC114322.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476791 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAACA CGGAGCGACT GAGAGTATTT GAACTATACA GCGGGATAGG AGGAATGCAT 
TATGCTTTAA AGGAGAGCTT AGTTCCAGCG GAAGTTGTAG CTGCTGTGGA TGTCAACACA 
ACAGCCAATC TAATCTACAA GCACAACTTC CCCACTACAC AACTCTTGCC AAAAACCATA 
GAGGGAATGA CGTTACAGGA TTTTGACAGG CTGAATTTTG ACATGATTTT GATGAGCCCA 
CCGTGTCAAC CTTTCACCAG GATTGGTTTG CAGGGAGATG TTGCCGACCC TAGAACAAAG 
AGCTTCCTGT ATATCCTGGA TCTTCTGCCA AGGCTCAGCA AGCGGCCACG CTTCATTCTG 
CTGGAGAATG TGAAGGGCTT TGAGAGCTCA GCTGCAAGGG ATGCTTTGCT TCAGACCCTG 
AGAGAGTGCG ACTACAGCTT TCAGGAGTTT CTGATTTCTC CAACATCTCT TGGGATCCCT 
AATTCTAGGC TCAGATACTT CCTGATTGCA AAGAGAGCAC CAGAAACCTT CTCTTTCCCT 
GTTTCAACTG AGATTATTGA GGGCTTCCCC ATGTCTGAAT CTACAGACGG TCTCTCTGTT 
CCATATGATC ATCCCACATC ATCTGCATCT GAGAGAGAAA AGACGATCAT GTTTAAGCTA 
GAGACGGAAG AAGATTTGGA AAGGAAGAGA AAGCAGAACA GTCAGGAATC AGTCAGGAGG 
TTGCTGGACT TTCTGCAGGA GGAGGAAGAG GACATGGAGC CGTATCTGCT GCCTCCCAAA 
ACCCTGCTCA GATACGCTCT GGTGATGGAT ATAGTTCAGC CAAGCAGCAG GAGATCGGTC 
TGCTTTACTA AAGGGTACGG TCATTATGTA GAGGGCACGG GCTCCGTCCT GCAGTCATGT 
ATGGATGTGG ATCTGGAGAC GGTGTTTAAG AATCTGGATC AGCTTTCAGA GGAAGACAAA 
CTCAAGCAGC TCCTCAGGCT CAAGCTCAGA TACTTCACTC CCAGAGAAAT CAGCAGACTC 
ATGGGCTTCC CGGATCATTT CACATTTCCA AAGCACATTT CCTTCAAACA GCAGTACCGT 
GTGTTGGGGA ACAGCCTGAA CGTCCACGTA GTTTCACATC TTATACGTCT AATGCTCTCA 
AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018153.1
CDS	66..1211
Translation	MENTERLRVFELYSGIGGMHYALKESLVPAEVVAAVDVNTTANLIYKHNFPTTQLLPKTIEGMTLQDFDRLNFDMILMSPPCQPFTRIGLQGDVADPRTKSFLYILDLLPRLSKRPRFILLENVKGFESSAARDALLQTLRECDYSFQEFLISPTSLGIPNSRLRYFLIAKRAPETFSFPVSTEIIEGFPMSESTDGLSVPYDHPTSSASEREKTIMFKLETEEDLERKRKQNSQESVRRLLDFLQEEEEDMEPYLLPPKTLLRYALVMDIVQPSSRRSVCFTKGYGHYVEGTGSVLQSCMDVDLETVFKNLDQLSEEDKLKQLLRLKLRYFTPREISRLMGFPDHFTFPKHISFKQQYRVLGNSLNVHVVSHLIRLMLSK

Target ORF information:

RefSeq Version	NM_001018143.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tRNA aspartic acid methyltransferase 1 (trdmt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018143.1

ORF Insert Sequence:

ATGGAGAACA CGGAGCGACT GAGAGTATTT GAACTATACA GCGGGATAGG AGGAATGCAT 
TATGCTTTAA AGGAGAGCTT AGTTCCAGCG GAAGTTGTAG CTGCTGTGGA TGTCAACACA 
ACAGCCAATC TAATCTACAA GCACAACTTC CCCACTACAC AACTCTTGCC AAAAACCATA 
GAGGGAATGA CGTTACAGGA TTTTGACAGG CTGAATTTTG ACATGATTTT GATGAGCCCA 
CCGTGTCAAC CTTTCACCAG GATTGGTTTG CAGGGAGATG TTGCCGACCC TAGAACAAAG 
AGCTTCCTGT ATATCCTGGA TCTTCTGCCA AGGCTCAGCA AGCGGCCACG CTTCATTCTG 
CTGGAGAATG TGAAGGGCTT TGAGAGCTCA GCTGCAAGGG ATGCTTTGCT TCAGACCCTG 
AGAGAGTGCG ACTACAGCTT TCAGGAGTTT CTGATTTCTC CAACATCTCT TGGGATCCCT 
AATTCTAGGC TCAGATACTT CCTGATTGCA AAGAGAGCAC CAGAAACCTT CTCTTTCCCT 
GTTTCAACTG AGATTATTGA GGGCTTCCCC ATGTCTGAAT CTACAGACGG TCTCTCTGTT 
CCATATGATC ATCCCACATC ATCTGCATCT GAGAGAGAAA AGACGATCAT GTTTAAGCTA 
GAGACGGAAG AAGATTTGGA AAGGAAGAGA AAGCAGAACA GTCAGGAATC AGTCAGGAGG 
TTGCTGGACT TTCTGCAGGA GGAGGAAGAG GACATGGAGC CGTATCTGCT GCCTCCCAAA 
ACCCTGCTCA GATACGCTCT GGTGATGGAT ATAGTTCAGC CAAGCAGCAG GAGATCGGTC 
TGCTTTACTA AAGGGTACGG TCATTATGTA GAGGGCACGG GCTCCGTCCT GCAGTCATGT 
ATGGATGTGG ATCTGGAGAC GGTGTTTAAG AATCTGGATC AGCTTTCAGA GGAAGACAAA 
CTCAAGCAGC TCCTCAGGCT CAAGCTCAGA TACTTCACTC CCAGAGAAAT CAGCAGACTC 
ATGGGCTTCC CGGATCATTT CACATTTCCA AAGCACATTT CCTTCAAACA GCAGTACCGT 
GTGTTGGGGA ACAGCCTGAA CGTCCACGTA GTTTCACATC TTATACGTCT AATGCTCTCA 
AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Partial depletion of yolk during zebrafish embryogenesis changes the dynamics of methionine cycle and metabolic genes.
BMC genomics16427(2015 Jun)
Huang Y,Linsen SE

Expression of the de novo DNA methyltransferases (dnmt3 - dnmt8) during zebrafish lens development.
Developmental dynamics : an official publication of the American Association of Anatomists243(2)350-6(2014 Feb)
Seritrakul P,Gross JM

Gonad differentiation in zebrafish is regulated by the canonical Wnt signaling pathway.
Biology of reproduction90(2)45(2014 Feb)
Sreenivasan R,Jiang J,Wang X,B?rtfai R,Kwan HY,Christoffels A,Orb?n L

Analysis of DNA methylation reveals a partial reprogramming of the M?ller glia genome during retina regeneration.
Proceedings of the National Academy of Sciences of the United States of America110(49)19814-9(2013 Dec)
Powell C,Grant AR,Cornblath E,Goldman D