vps33a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	vps33a
Entrez Gene ID	448942
Full Name	vacuolar protein sorting 33A
Synonyms	im:7136893,wu:fc08g01,wu:fc26c10
General protein information	Preferred Names vacuolar protein sorting 33A Names vacuolar protein sorting-associated protein 33A vacuolar protein sorting 33 homolog A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001099973.1	NP_001093443.1	vacuolar protein sorting-associated protein 33A

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	vps33a	NP_001093443.1
Drosophila melanogaster (fruit fly)	car	NP_001259707.1
Magnaporthe oryzae (rice blast fungus)	MGG_09914	XP_003710012.1
Arabidopsis thaliana (thale cress)	ATVPS33	NP_001154679.1
Oryza sativa (rice)	Os04g0223000	NP_001052270.1
Homo sapiens (human)	VPS33A	NP_075067.2
Pan troglodytes (chimpanzee)	VPS33A	XP_001167604.1
	VPS33A	XP_001098683.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003203	XP_312907.4
Canis lupus familiaris (dog)	VPS33A	XP_534660.2
Gallus gallus (chicken)	VPS33A	XP_415153.1
Bos taurus (cattle)	VPS33A	NP_001039514.1
Rattus norvegicus (Norway rat)	Vps33a	NP_075250.2
Neurospora crassa	NCU09121	XP_958893.1
Mus musculus (house mouse)	Vps33a	NP_084205.3
Schizosaccharomyces pombe (fission yeast)	vps33	NP_596210.1
Xenopus tropicalis (tropical clawed frog)	vps33a	NP_001016935.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Genetic characterization and in vivo image analysis of novel zebrafish Danio rerio pigment mutants.
Hsu CC, Pai WY, Lai CY, Lu MW, Her GM
Journal of fish biology82(5)1671-83(2013 May)

Zebrafish vps33b, an ortholog of the gene responsible for human arthrogryposis-renal dysfunction-cholestasis syndrome, regulates biliary development downstream of the onecut transcription factor hnf6.
Matthews RP, Plumb-Rudewiez N, Lorent K, Gissen P, Johnson CA, Lemaigre F, Pack M
Development (Cambridge, England)132(23)5295-306(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following vps33a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vps33a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa04116	NM_001099973.1 Latest version!	Danio rerio VPS33A core subunit of CORVET and HOPS complexes (vps33a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vps33a ( NM_001099973.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001093443.1 Danio rerio VPS33A core subunit of CORVET and HOPS complexes (vps33a), mRNA.

Price & Availability↑

CloneID	ODa04116
Clone ID Related Accession (Same CDS sequence)	NM_001099973.1
Accession Version	NM_001099973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	vacuolar protein sorting-associated protein 33A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CT978957.10. On Jul 26, 2007 this sequence version replaced XM_678109.2. ##Evidence-Data-START## Transcript exon combination :: BC095344.1, GDQQ01021446.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTCAC ATTTGTTTTG CGGTAGAGTA AACCTAAATA TTTTGAGAGA GGCTGCACGA 
AAAGACCTGC GCGAGTTTTT GGATAAATGT TCGGGAAGCA AGGCCATTGT TTGGGATGAG 
TATCTAACAG GACCTTTCGG GCTGATTGCT CAGTATTCAC TTTTGAAGGA ACATGAAGTG 
GAGAAAATGT TCACTCTTAA AGGAGGCCGA ATACCATCTG CCGCCGTCAA GAATATTGTC 
TTCTTTGTTC GCCCGAGGTT GGAGTTAATG GACATCATTG CAGAAAATGT TTCGAGTGAG 
GATAAACTGC AGCCTCGTGA GTTTCACATT TTGTTCGTGC CTCGCCGGAG CCTCCTGTGT 
GAGCAGCGGC TGAAGGAGAA AGGCGTCCTG AACTCCTTCA CAAACATCGA CGAGTACATC 
CTCGACCTCA TCCCTTATGA TGGAGATCTG CTTTCCATGG AGTCTGAGAG CTCCTTTAGA 
GAGTGTTATT TAGAAAATGA TCAGACGAGC CTCTATCATG CTGCCAAAGG CCTCATGACT 
TTGCAAGCAC TTTATGGAAC AATACCACAG ATATTTGGCA AAGGAGAGTG TGCACGGCAT 
GTGGCCAACA TGATGCTGCG GATGAAGAGA GAGTTTGCAG GCAGCCACAC TCAGATCCTG 
CCTGTGTTTG ACACTCTTCT GCTGCTGGAT CGAAACGTGG ATTTACTCAC ACCGCTCGCC 
ACGCAGCTCA CATACGAGGG TCTCATCGAC GAGATCTTTG GCATCACCAA TGGTTATGTG 
AAGCTCCCGC CTGAAAAGTT TGCTCAGAAG AAACAGGGTG AAGGCGGCAA AGATCTGCCC 
ACCGAGCCCA AAAAACTGCA GCTGAACTCC GCTGAGGAGC TTTACGCCGA GATACGCGAC 
AAGAACTTCA ATGCTGTCGG TGCGGCTCTC AGCAAGAAGG CCAAGATTAT TTCAGCAGCG 
TTTGAGGAGC GACACAACGC CAAAACTGTG GGAGAAATTA AGCAGTTTGT TTCCCAGCTG 
CCGCACATGC AGGCGGCGCG CAGCTCTCTG GCCAATCACA CCTCCATCGC TGAACTTATC 
AAAGACATCA CCACTTCAGA GGTTTTCTTT GACAGTCTGA CGGTCGAGCA GGAGTTCATG 
ACTGGAGTCG ACACAGACAA GGTCAGCACT TATATTGAGG ACTGCATTGC GCAGAAAGAC 
CCGCTGATAA AAATCCTGCG GCTGGTCTGT ATGCAGTCTG TCTGCAACAA CGGACTCAAA 
CAGAAAGTTC TGGACTACTA TAAGAGGGAG ATCCTTCAGA CGTACGGTTA TAAGCACATT 
CTGACCCTCA AGAACTTGGA GAAGGTTGGT TTGCTCAAAC CTCAGAGCAC CATGAGGAAC 
AACTACCCCA CAATCAGGAA AACACTCAAG CTGTGGATGG AGGACGCCAA CGAACAGAAC 
CCGAATGATA TCTCGTACGT GTACAGCGGC TACGCTCCGC TCAGCGTTCG CCTCACTCAG 
GTTCTGGCGC GACCAGGATG GAGGAGCATC GAGGAGGTGC TGAAGATGCT GCCTGGGCCA 
CACTTTGAGG AACGACAGCA GGTTCCCACA GGCCTCCACA AGAAGCGTCA GCCTGGAGAA 
AACCGCACCA CTTTAGTCTT CTTTCTGGGC GGCGTGACGT ACGCTGAGAT CGCCGCTCTG 
CGTTTTCTCT CTCATATGGA GGACAGCGGG ACCGAGTACA TCATCGCCAC CACCAAACTG 
ATCAACGGCA CCAGCTGGGT CAAATCTCTG ATGGATCACC CAGAGGAAAA CCAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093443.1
CDS	27..1826
Translation	MASHLFCGRVNLNILREAARKDLREFLDKCSGSKAIVWDEYLTGPFGLIAQYSLLKEHEVEKMFTLKGGRIPSAAVKNIVFFVRPRLELMDIIAENVSSEDKLQPREFHILFVPRRSLLCEQRLKEKGVLNSFTNIDEYILDLIPYDGDLLSMESESSFRECYLENDQTSLYHAAKGLMTLQALYGTIPQIFGKGECARHVANMMLRMKREFAGSHTQILPVFDTLLLLDRNVDLLTPLATQLTYEGLIDEIFGITNGYVKLPPEKFAQKKQGEGGKDLPTEPKKLQLNSAEELYAEIRDKNFNAVGAALSKKAKIISAAFEERHNAKTVGEIKQFVSQLPHMQAARSSLANHTSIAELIKDITTSEVFFDSLTVEQEFMTGVDTDKVSTYIEDCIAQKDPLIKILRLVCMQSVCNNGLKQKVLDYYKREILQTYGYKHILTLKNLEKVGLLKPQSTMRNNYPTIRKTLKLWMEDANEQNPNDISYVYSGYAPLSVRLTQVLARPGWRSIEEVLKMLPGPHFEERQQVPTGLHKKRQPGENRTTLVFFLGGVTYAEIAALRFLSHMEDSGTEYIIATTKLINGTSWVKSLMDHPEENQQ

Target ORF information:

RefSeq Version	NM_001099973.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio VPS33A core subunit of CORVET and HOPS complexes (vps33a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099973.1

ORF Insert Sequence:

ATGGCGTCAC ATTTGTTTTG CGGTAGAGTA AACCTAAATA TTTTGAGAGA GGCTGCACGA 
AAAGACCTGC GCGAGTTTTT GGATAAATGT TCGGGAAGCA AGGCCATTGT TTGGGATGAG 
TATCTAACAG GACCTTTCGG GCTGATTGCT CAGTATTCAC TTTTGAAGGA ACATGAAGTG 
GAGAAAATGT TCACTCTTAA AGGAGGCCGA ATACCATCTG CCGCCGTCAA GAATATTGTC 
TTCTTTGTTC GCCCGAGGTT GGAGTTAATG GACATCATTG CAGAAAATGT TTCGAGTGAG 
GATAAACTGC AGCCTCGTGA GTTTCACATT TTGTTCGTGC CTCGCCGGAG CCTCCTGTGT 
GAGCAGCGGC TGAAGGAGAA AGGCGTCCTG AACTCCTTCA CAAACATCGA CGAGTACATC 
CTCGACCTCA TCCCTTATGA TGGAGATCTG CTTTCCATGG AGTCTGAGAG CTCCTTTAGA 
GAGTGTTATT TAGAAAATGA TCAGACGAGC CTCTATCATG CTGCCAAAGG CCTCATGACT 
TTGCAAGCAC TTTATGGAAC AATACCACAG ATATTTGGCA AAGGAGAGTG TGCACGGCAT 
GTGGCCAACA TGATGCTGCG GATGAAGAGA GAGTTTGCAG GCAGCCACAC TCAGATCCTG 
CCTGTGTTTG ACACTCTTCT GCTGCTGGAT CGAAACGTGG ATTTACTCAC ACCGCTCGCC 
ACGCAGCTCA CATACGAGGG TCTCATCGAC GAGATCTTTG GCATCACCAA TGGTTATGTG 
AAGCTCCCGC CTGAAAAGTT TGCTCAGAAG AAACAGGGTG AAGGCGGCAA AGATCTGCCC 
ACCGAGCCCA AAAAACTGCA GCTGAACTCC GCTGAGGAGC TTTACGCCGA GATACGCGAC 
AAGAACTTCA ATGCTGTCGG TGCGGCTCTC AGCAAGAAGG CCAAGATTAT TTCAGCAGCG 
TTTGAGGAGC GACACAACGC CAAAACTGTG GGAGAAATTA AGCAGTTTGT TTCCCAGCTG 
CCGCACATGC AGGCGGCGCG CAGCTCTCTG GCCAATCACA CCTCCATCGC TGAACTTATC 
AAAGACATCA CCACTTCAGA GGTTTTCTTT GACAGTCTGA CGGTCGAGCA GGAGTTCATG 
ACTGGAGTCG ACACAGACAA GGTCAGCACT TATATTGAGG ACTGCATTGC GCAGAAAGAC 
CCGCTGATAA AAATCCTGCG GCTGGTCTGT ATGCAGTCTG TCTGCAACAA CGGACTCAAA 
CAGAAAGTTC TGGACTACTA TAAGAGGGAG ATCCTTCAGA CGTACGGTTA TAAGCACATT 
CTGACCCTCA AGAACTTGGA GAAGGTTGGT TTGCTCAAAC CTCAGAGCAC CATGAGGAAC 
AACTACCCCA CAATCAGGAA AACACTCAAG CTGTGGATGG AGGACGCCAA CGAACAGAAC 
CCGAATGATA TCTCGTACGT GTACAGCGGC TACGCTCCGC TCAGCGTTCG CCTCACTCAG 
GTTCTGGCGC GACCAGGATG GAGGAGCATC GAGGAGGTGC TGAAGATGCT GCCTGGGCCA 
CACTTTGAGG AACGACAGCA GGTTCCCACA GGCCTCCACA AGAAGCGTCA GCCTGGAGAA 
AACCGCACCA CTTTAGTCTT CTTTCTGGGC GGCGTGACGT ACGCTGAGAT CGCCGCTCTG 
CGTTTTCTCT CTCATATGGA GGACAGCGGG ACCGAGTACA TCATCGCCAC CACCAAACTG 
ATCAACGGCA CCAGCTGGGT CAAATCTCTG ATGGATCACC CAGAGGAAAA CCAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Genetic characterization and in vivo image analysis of novel zebrafish Danio rerio pigment mutants.
Journal of fish biology82(5)1671-83(2013 May)
Hsu CC,Pai WY,Lai CY,Lu MW,Her GM

Zebrafish vps33b, an ortholog of the gene responsible for human arthrogryposis-renal dysfunction-cholestasis syndrome, regulates biliary development downstream of the onecut transcription factor hnf6.
Development (Cambridge, England)132(23)5295-306(2005 Dec)
Matthews RP,Plumb-Rudewiez N,Lorent K,Gissen P,Johnson CA,Lemaigre F,Pack M