rassf1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rassf1
Entrez Gene ID	447811
Full Name	Ras association (RalGDS/AF-6) domain family 1
Synonyms	wu:fc17f02,zgc:112224,zgc:92505
General protein information	Preferred Names Ras association (RalGDS/AF-6) domain family 1 Names ras association domain-containing protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005173951.4	XP_005174008.1	ras association domain-containing protein 1 isoform X1
XM_005173952.3	XP_005174009.1	ras association domain-containing protein 1 isoform X2
NM_001004550.1	NP_001004550.1	ras association domain-containing protein 1
XM_005173951.3	XP_005174008.1	ras association domain-containing protein 1 isoform X1
XM_017353113.2	XP_017208602.1	ras association domain-containing protein 1 isoform X1
XM_017353113.1	XP_017208602.1	ras association domain-containing protein 1 isoform X1
XM_005173952.4	XP_005174009.1	ras association domain-containing protein 1 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RASSF1	XP_005632562.1
Rattus norvegicus (Norway rat)	Rassf1	NP_001032644.1
Gallus gallus (chicken)	RASSF1	XP_004944511.1
Danio rerio (zebrafish)	rassf1	NP_001004550.1
	RASSF1	XP_001100583.2
Pan troglodytes (chimpanzee)	RASSF1	XP_001168270.2
Caenorhabditis elegans (roundworm)	T24F1.3	NP_001022362.1
Homo sapiens (human)	RASSF1	NP_009113.3
Mus musculus (house mouse)	Rassf1	NP_001230677.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Global and gene specific DNA methylation changes during zebrafish development.
Fang X, Corrales J, Thornton C, Scheffler BE, Willett KL
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology166(1)99-108(2013 Sep)

Benzo[a]pyrene decreases global and gene specific DNA methylation during zebrafish development.
Fang X, Thornton C, Scheffler BE, Willett KL
Environmental toxicology and pharmacology36(1)40-50(2013 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rassf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rassf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa26675	NM_001004550.1 Latest version!	Danio rerio Ras association domain family member 1 (rassf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa26675	XM_005173951.4 Latest version!	Danio rerio Ras association (RalGDS/AF-6) domain family 1 (rassf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa26675	XM_017353113.2 Latest version!	Danio rerio Ras association (RalGDS/AF-6) domain family 1 (rassf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa26676	XM_005173952.4 Latest version!	Danio rerio Ras association (RalGDS/AF-6) domain family 1 (rassf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa26675	XM_005173951.3	Danio rerio Ras association (RalGDS/AF-6) domain family 1 (rassf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa26675	XM_017353113.1	Danio rerio Ras association (RalGDS/AF-6) domain family 1 (rassf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa26676	XM_005173952.3	Danio rerio Ras association (RalGDS/AF-6) domain family 1 (rassf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rassf1 ( NM_001004550.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001004550.1 Danio rerio Ras association domain family member 1 (rassf1), mRNA.

Price & Availability↑

CloneID	ODa26675
Clone ID Related Accession (Same CDS sequence)	XM_005173951.4 , NM_001004550.1 , XM_005173951.3 , XM_017353113.2 , XM_017353113.1
Accession Version	NM_001004550.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-29
Organism	Danio rerio(zebrafish)
Product	ras association domain-containing protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081661.1. ##Evidence-Data-START## Transcript exon combination :: BC081661.1, CK027942.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCAAAAT GTGAGCTCAT CGAGTTGCAG GACTTGACTC CGAATGACCG TATTGAGCTG 
GCACCCCCTA GTGTCCCTCC ACCCACCGTG GTGCCCACTC TGGACAGGTG GAGCAGAGGG 
AAGGTGGTGC GCATGGTGGG CGAGCGCGTG CGCCTGGAGG ACCCCGATTG GCTGACGTGT 
AAACCAGGAC GAGGACATGA CTTTCAGCCC TGCAGCCAGA CACAGCTGAG CTGGTGTGAC 
CTGTGTGGAG AGTTCATCTG GGGCCTGTAC AGACAGAGCC TCCGCTGCAC ACACTGTAAC 
TACACTTGTC ACTACCGCTG TCAACCCTTC ATTCAGCTGG ACTGCAGCTC CAACACCGAC 
ACTATCTGCG AACAATCAAA CTACAGCGAG GACACCATCG AGACAGACAC CAATGTGGAT 
GAGCAGTCTG AAGTGGACTG GAGGAAACAG GATCTGTCTG TCACTGAAAT ACAGCAGAAA 
GTGAAGGAAT ACAATGCTCA GGTCAACAGT AACCTCTTCA TGGTTCTGAA TCGTGACGGC 
TCATACACTG GCTTCATCAA GGTCCAGTTT AAGCTGGCGC GACCCGTGTC TCTTCCTCCT 
CCCCGCAGCG TCTCCTCCTC CTCCATCTCC TCCTCTTGTT TAGGATGGGA TGGCGGCTGT 
CAGGAGCGAA CTTCCTTCTA CCTGCCCAGA GACACAGTCA AGCACCTGCA CATCAGCTCC 
AGCACCCGTG CCAGAGAGGT CATCCAGGCC CTGCTCAACA AGTTCACTGT GGTGGACAAT 
CCGGCTAAAT ATTCCCTGTA TGAGCGCAGC CAGCGGGACA ATCAAGTGTA CTTAAGGAAG 
TTAGCTGATG ATGAATGTCC ACTTTTCCTG CGTCTGTGTG CTGGACCCAA TGAGAAAGTC 
CTGAGTTTAG TGCTTAAAGA GAATGAAACC GGGGAAGTGA ATTGGGATGC GTTCAGTTTT 
CCTGAACTCC AGAACTTCCT GCGGATTCTC CAGCGGGAGG AAGAAGATCA CGTCCGGCAA 
ATCATACGCC GATACACTCT GGCTCGTGAT AAGATGAAAG AGGCTATGAA GAACTTCAGC 
AAGCCTGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004550.1
CDS	45..1136
Translation	MAKCELIELQDLTPNDRIELAPPSVPPPTVVPTLDRWSRGKVVRMVGERVRLEDPDWLTCKPGRGHDFQPCSQTQLSWCDLCGEFIWGLYRQSLRCTHCNYTCHYRCQPFIQLDCSSNTDTICEQSNYSEDTIETDTNVDEQSEVDWRKQDLSVTEIQQKVKEYNAQVNSNLFMVLNRDGSYTGFIKVQFKLARPVSLPPPRSVSSSSISSSCLGWDGGCQERTSFYLPRDTVKHLHISSSTRAREVIQALLNKFTVVDNPAKYSLYERSQRDNQVYLRKLADDECPLFLRLCAGPNEKVLSLVLKENETGEVNWDAFSFPELQNFLRILQREEEDHVRQIIRRYTLARDKMKEAMKNFSKPG

Target ORF information:

RefSeq Version	NM_001004550.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ras association domain family member 1 (rassf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004550.1

ORF Insert Sequence:

ATGGCAAAAT GTGAGCTCAT CGAGTTGCAG GACTTGACTC CGAATGACCG TATTGAGCTG 
GCACCCCCTA GTGTCCCTCC ACCCACCGTG GTGCCCACTC TGGACAGGTG GAGCAGAGGG 
AAGGTGGTGC GCATGGTGGG CGAGCGCGTG CGCCTGGAGG ACCCCGATTG GCTGACGTGT 
AAACCAGGAC GAGGACATGA CTTTCAGCCC TGCAGCCAGA CACAGCTGAG CTGGTGTGAC 
CTGTGTGGAG AGTTCATCTG GGGCCTGTAC AGACAGAGCC TCCGCTGCAC ACACTGTAAC 
TACACTTGTC ACTACCGCTG TCAACCCTTC ATTCAGCTGG ACTGCAGCTC CAACACCGAC 
ACTATCTGCG AACAATCAAA CTACAGCGAG GACACCATCG AGACAGACAC CAATGTGGAT 
GAGCAGTCTG AAGTGGACTG GAGGAAACAG GATCTGTCTG TCACTGAAAT ACAGCAGAAA 
GTGAAGGAAT ACAATGCTCA GGTCAACAGT AACCTCTTCA TGGTTCTGAA TCGTGACGGC 
TCATACACTG GCTTCATCAA GGTCCAGTTT AAGCTGGCGC GACCCGTGTC TCTTCCTCCT 
CCCCGCAGCG TCTCCTCCTC CTCCATCTCC TCCTCTTGTT TAGGATGGGA TGGCGGCTGT 
CAGGAGCGAA CTTCCTTCTA CCTGCCCAGA GACACAGTCA AGCACCTGCA CATCAGCTCC 
AGCACCCGTG CCAGAGAGGT CATCCAGGCC CTGCTCAACA AGTTCACTGT GGTGGACAAT 
CCGGCTAAAT ATTCCCTGTA TGAGCGCAGC CAGCGGGACA ATCAAGTGTA CTTAAGGAAG 
TTAGCTGATG ATGAATGTCC ACTTTTCCTG CGTCTGTGTG CTGGACCCAA TGAGAAAGTC 
CTGAGTTTAG TGCTTAAAGA GAATGAAACC GGGGAAGTGA ATTGGGATGC GTTCAGTTTT 
CCTGAACTCC AGAACTTCCT GCGGATTCTC CAGCGGGAGG AAGAAGATCA CGTCCGGCAA 
ATCATACGCC GATACACTCT GGCTCGTGAT AAGATGAAAG AGGCTATGAA GAACTTCAGC 
AAGCCTGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rassf1 ( XM_005173951.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005174008.1 Danio rerio Ras association (RalGDS/AF-6) domain family 1 (rassf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26675
Clone ID Related Accession (Same CDS sequence)	XM_005173951.4 , NM_001004550.1 , XM_005173951.3 , XM_017353113.2 , XM_017353113.1
Accession Version	XM_005173951.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras association domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173951.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAT GTGAGCTCAT CGAGTTGCAG GACTTGACTC CGAATGACCG TATTGAGCTG 
GCACCCCCTA GTGTCCCTCC ACCCACCGTG GTGCCCACTC TGGACAGGTG GAGCAGAGGG 
AAGGTGGTGC GCATGGTGGG CGAGCGCGTG CGCCTGGAGG ACCCCGATTG GCTGACGTGT 
AAACCAGGAC GAGGACATGA CTTTCAGCCC TGCAGCCAGA CACAGCTGAG CTGGTGTGAC 
CTGTGTGGAG AGTTCATCTG GGGCCTGTAC AGACAGAGCC TCCGCTGCAC ACACTGTAAC 
TACACTTGTC ACTACCGCTG TCAACCCTTC ATTCAGCTGG ACTGCAGCTC CAACACCGAC 
ACTATCTGCG AACAATCAAA CTACAGCGAG GACACCATCG AGACAGACAC CAATGTGGAT 
GAGCAGTCTG AAGTGGACTG GAGGAAACAG GATCTGTCTG TCACTGAAAT ACAGCAGAAA 
GTGAAGGAAT ACAATGCTCA GGTCAACAGT AACCTCTTCA TGGTTCTGAA TCGTGACGGC 
TCATACACTG GCTTCATCAA GGTCCAGTTT AAGCTGGCGC GACCCGTGTC TCTTCCTCCT 
CCCCGCAGCG TCTCCTCCTC CTCCATCTCC TCCTCTTGTT TAGGATGGGA TGGCGGCTGT 
CAGGAGCGAA CTTCCTTCTA CCTGCCCAGA GACACAGTCA AGCACCTGCA CATCAGCTCC 
AGCACCCGTG CCAGAGAGGT CATCCAGGCC CTGCTCAACA AGTTCACTGT GGTGGACAAT 
CCGGCTAAAT ATTCCCTGTA TGAGCGCAGC CAGCGGGACA ATCAAGTGTA CTTAAGGAAG 
TTAGCTGATG ATGAATGTCC ACTTTTCCTG CGTCTGTGTG CTGGACCCAA TGAGAAAGTC 
CTGAGTTTAG TGCTTAAAGA GAATGAAACC GGGGAAGTGA ATTGGGATGC GTTCAGTTTT 
CCTGAACTCC AGAACTTCCT GCGGATTCTC CAGCGGGAGG AAGAAGATCA CGTCCGGCAA 
ATCATACGCC GATACACTCT GGCTCGTGAT AAGATGAAAG AGGCTATGAA GAACTTCAGC 
AAGCCTGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174008.1
CDS	687..1778
Translation	MAKCELIELQDLTPNDRIELAPPSVPPPTVVPTLDRWSRGKVVRMVGERVRLEDPDWLTCKPGRGHDFQPCSQTQLSWCDLCGEFIWGLYRQSLRCTHCNYTCHYRCQPFIQLDCSSNTDTICEQSNYSEDTIETDTNVDEQSEVDWRKQDLSVTEIQQKVKEYNAQVNSNLFMVLNRDGSYTGFIKVQFKLARPVSLPPPRSVSSSSISSSCLGWDGGCQERTSFYLPRDTVKHLHISSSTRAREVIQALLNKFTVVDNPAKYSLYERSQRDNQVYLRKLADDECPLFLRLCAGPNEKVLSLVLKENETGEVNWDAFSFPELQNFLRILQREEEDHVRQIIRRYTLARDKMKEAMKNFSKPG

Target ORF information:

RefSeq Version	XM_005173951.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) domain family 1 (rassf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173951.4

ORF Insert Sequence:

ATGGCAAAAT GTGAGCTCAT CGAGTTGCAG GACTTGACTC CGAATGACCG TATTGAGCTG 
GCACCCCCTA GTGTCCCTCC ACCCACCGTG GTGCCCACTC TGGACAGGTG GAGCAGAGGG 
AAGGTGGTGC GCATGGTGGG CGAGCGCGTG CGCCTGGAGG ACCCCGATTG GCTGACGTGT 
AAACCAGGAC GAGGACATGA CTTTCAGCCC TGCAGCCAGA CACAGCTGAG CTGGTGTGAC 
CTGTGTGGAG AGTTCATCTG GGGCCTGTAC AGACAGAGCC TCCGCTGCAC ACACTGTAAC 
TACACTTGTC ACTACCGCTG TCAACCCTTC ATTCAGCTGG ACTGCAGCTC CAACACCGAC 
ACTATCTGCG AACAATCAAA CTACAGCGAG GACACCATCG AGACAGACAC CAATGTGGAT 
GAGCAGTCTG AAGTGGACTG GAGGAAACAG GATCTGTCTG TCACTGAAAT ACAGCAGAAA 
GTGAAGGAAT ACAATGCTCA GGTCAACAGT AACCTCTTCA TGGTTCTGAA TCGTGACGGC 
TCATACACTG GCTTCATCAA GGTCCAGTTT AAGCTGGCGC GACCCGTGTC TCTTCCTCCT 
CCCCGCAGCG TCTCCTCCTC CTCCATCTCC TCCTCTTGTT TAGGATGGGA TGGCGGCTGT 
CAGGAGCGAA CTTCCTTCTA CCTGCCCAGA GACACAGTCA AGCACCTGCA CATCAGCTCC 
AGCACCCGTG CCAGAGAGGT CATCCAGGCC CTGCTCAACA AGTTCACTGT GGTGGACAAT 
CCGGCTAAAT ATTCCCTGTA TGAGCGCAGC CAGCGGGACA ATCAAGTGTA CTTAAGGAAG 
TTAGCTGATG ATGAATGTCC ACTTTTCCTG CGTCTGTGTG CTGGACCCAA TGAGAAAGTC 
CTGAGTTTAG TGCTTAAAGA GAATGAAACC GGGGAAGTGA ATTGGGATGC GTTCAGTTTT 
CCTGAACTCC AGAACTTCCT GCGGATTCTC CAGCGGGAGG AAGAAGATCA CGTCCGGCAA 
ATCATACGCC GATACACTCT GGCTCGTGAT AAGATGAAAG AGGCTATGAA GAACTTCAGC 
AAGCCTGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rassf1 ( XM_005173951.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005174008.1 Danio rerio Ras association (RalGDS/AF-6) domain family 1 (rassf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26675
Clone ID Related Accession (Same CDS sequence)	XM_005173951.4 , NM_001004550.1 , XM_005173951.3 , XM_017353113.2 , XM_017353113.1
Accession Version	XM_005173951.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras association domain-containing protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173951.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAT GTGAGCTCAT CGAGTTGCAG GACTTGACTC CGAATGACCG TATTGAGCTG 
GCACCCCCTA GTGTCCCTCC ACCCACCGTG GTGCCCACTC TGGACAGGTG GAGCAGAGGG 
AAGGTGGTGC GCATGGTGGG CGAGCGCGTG CGCCTGGAGG ACCCCGATTG GCTGACGTGT 
AAACCAGGAC GAGGACATGA CTTTCAGCCC TGCAGCCAGA CACAGCTGAG CTGGTGTGAC 
CTGTGTGGAG AGTTCATCTG GGGCCTGTAC AGACAGAGCC TCCGCTGCAC ACACTGTAAC 
TACACTTGTC ACTACCGCTG TCAACCCTTC ATTCAGCTGG ACTGCAGCTC CAACACCGAC 
ACTATCTGCG AACAATCAAA CTACAGCGAG GACACCATCG AGACAGACAC CAATGTGGAT 
GAGCAGTCTG AAGTGGACTG GAGGAAACAG GATCTGTCTG TCACTGAAAT ACAGCAGAAA 
GTGAAGGAAT ACAATGCTCA GGTCAACAGT AACCTCTTCA TGGTTCTGAA TCGTGACGGC 
TCATACACTG GCTTCATCAA GGTCCAGTTT AAGCTGGCGC GACCCGTGTC TCTTCCTCCT 
CCCCGCAGCG TCTCCTCCTC CTCCATCTCC TCCTCTTGTT TAGGATGGGA TGGCGGCTGT 
CAGGAGCGAA CTTCCTTCTA CCTGCCCAGA GACACAGTCA AGCACCTGCA CATCAGCTCC 
AGCACCCGTG CCAGAGAGGT CATCCAGGCC CTGCTCAACA AGTTCACTGT GGTGGACAAT 
CCGGCTAAAT ATTCCCTGTA TGAGCGCAGC CAGCGGGACA ATCAAGTGTA CTTAAGGAAG 
TTAGCTGATG ATGAATGTCC ACTTTTCCTG CGTCTGTGTG CTGGACCCAA TGAGAAAGTC 
CTGAGTTTAG TGCTTAAAGA GAATGAAACC GGGGAAGTGA ATTGGGATGC GTTCAGTTTT 
CCTGAACTCC AGAACTTCCT GCGGATTCTC CAGCGGGAGG AAGAAGATCA CGTCCGGCAA 
ATCATACGCC GATACACTCT GGCTCGTGAT AAGATGAAAG AGGCTATGAA GAACTTCAGC 
AAGCCTGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174008.1
CDS	680..1771
Translation	MAKCELIELQDLTPNDRIELAPPSVPPPTVVPTLDRWSRGKVVRMVGERVRLEDPDWLTCKPGRGHDFQPCSQTQLSWCDLCGEFIWGLYRQSLRCTHCNYTCHYRCQPFIQLDCSSNTDTICEQSNYSEDTIETDTNVDEQSEVDWRKQDLSVTEIQQKVKEYNAQVNSNLFMVLNRDGSYTGFIKVQFKLARPVSLPPPRSVSSSSISSSCLGWDGGCQERTSFYLPRDTVKHLHISSSTRAREVIQALLNKFTVVDNPAKYSLYERSQRDNQVYLRKLADDECPLFLRLCAGPNEKVLSLVLKENETGEVNWDAFSFPELQNFLRILQREEEDHVRQIIRRYTLARDKMKEAMKNFSKPG

Target ORF information:

RefSeq Version	XM_005173951.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) domain family 1 (rassf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173951.3

ORF Insert Sequence:

ATGGCAAAAT GTGAGCTCAT CGAGTTGCAG GACTTGACTC CGAATGACCG TATTGAGCTG 
GCACCCCCTA GTGTCCCTCC ACCCACCGTG GTGCCCACTC TGGACAGGTG GAGCAGAGGG 
AAGGTGGTGC GCATGGTGGG CGAGCGCGTG CGCCTGGAGG ACCCCGATTG GCTGACGTGT 
AAACCAGGAC GAGGACATGA CTTTCAGCCC TGCAGCCAGA CACAGCTGAG CTGGTGTGAC 
CTGTGTGGAG AGTTCATCTG GGGCCTGTAC AGACAGAGCC TCCGCTGCAC ACACTGTAAC 
TACACTTGTC ACTACCGCTG TCAACCCTTC ATTCAGCTGG ACTGCAGCTC CAACACCGAC 
ACTATCTGCG AACAATCAAA CTACAGCGAG GACACCATCG AGACAGACAC CAATGTGGAT 
GAGCAGTCTG AAGTGGACTG GAGGAAACAG GATCTGTCTG TCACTGAAAT ACAGCAGAAA 
GTGAAGGAAT ACAATGCTCA GGTCAACAGT AACCTCTTCA TGGTTCTGAA TCGTGACGGC 
TCATACACTG GCTTCATCAA GGTCCAGTTT AAGCTGGCGC GACCCGTGTC TCTTCCTCCT 
CCCCGCAGCG TCTCCTCCTC CTCCATCTCC TCCTCTTGTT TAGGATGGGA TGGCGGCTGT 
CAGGAGCGAA CTTCCTTCTA CCTGCCCAGA GACACAGTCA AGCACCTGCA CATCAGCTCC 
AGCACCCGTG CCAGAGAGGT CATCCAGGCC CTGCTCAACA AGTTCACTGT GGTGGACAAT 
CCGGCTAAAT ATTCCCTGTA TGAGCGCAGC CAGCGGGACA ATCAAGTGTA CTTAAGGAAG 
TTAGCTGATG ATGAATGTCC ACTTTTCCTG CGTCTGTGTG CTGGACCCAA TGAGAAAGTC 
CTGAGTTTAG TGCTTAAAGA GAATGAAACC GGGGAAGTGA ATTGGGATGC GTTCAGTTTT 
CCTGAACTCC AGAACTTCCT GCGGATTCTC CAGCGGGAGG AAGAAGATCA CGTCCGGCAA 
ATCATACGCC GATACACTCT GGCTCGTGAT AAGATGAAAG AGGCTATGAA GAACTTCAGC 
AAGCCTGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rassf1 ( XM_017353113.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208602.1 Danio rerio Ras association (RalGDS/AF-6) domain family 1 (rassf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa26675
Clone ID Related Accession (Same CDS sequence)	XM_005173951.4 , NM_001004550.1 , XM_005173951.3 , XM_017353113.2 , XM_017353113.1
Accession Version	XM_017353113.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras association domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353113.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAT GTGAGCTCAT CGAGTTGCAG GACTTGACTC CGAATGACCG TATTGAGCTG 
GCACCCCCTA GTGTCCCTCC ACCCACCGTG GTGCCCACTC TGGACAGGTG GAGCAGAGGG 
AAGGTGGTGC GCATGGTGGG CGAGCGCGTG CGCCTGGAGG ACCCCGATTG GCTGACGTGT 
AAACCAGGAC GAGGACATGA CTTTCAGCCC TGCAGCCAGA CACAGCTGAG CTGGTGTGAC 
CTGTGTGGAG AGTTCATCTG GGGCCTGTAC AGACAGAGCC TCCGCTGCAC ACACTGTAAC 
TACACTTGTC ACTACCGCTG TCAACCCTTC ATTCAGCTGG ACTGCAGCTC CAACACCGAC 
ACTATCTGCG AACAATCAAA CTACAGCGAG GACACCATCG AGACAGACAC CAATGTGGAT 
GAGCAGTCTG AAGTGGACTG GAGGAAACAG GATCTGTCTG TCACTGAAAT ACAGCAGAAA 
GTGAAGGAAT ACAATGCTCA GGTCAACAGT AACCTCTTCA TGGTTCTGAA TCGTGACGGC 
TCATACACTG GCTTCATCAA GGTCCAGTTT AAGCTGGCGC GACCCGTGTC TCTTCCTCCT 
CCCCGCAGCG TCTCCTCCTC CTCCATCTCC TCCTCTTGTT TAGGATGGGA TGGCGGCTGT 
CAGGAGCGAA CTTCCTTCTA CCTGCCCAGA GACACAGTCA AGCACCTGCA CATCAGCTCC 
AGCACCCGTG CCAGAGAGGT CATCCAGGCC CTGCTCAACA AGTTCACTGT GGTGGACAAT 
CCGGCTAAAT ATTCCCTGTA TGAGCGCAGC CAGCGGGACA ATCAAGTGTA CTTAAGGAAG 
TTAGCTGATG ATGAATGTCC ACTTTTCCTG CGTCTGTGTG CTGGACCCAA TGAGAAAGTC 
CTGAGTTTAG TGCTTAAAGA GAATGAAACC GGGGAAGTGA ATTGGGATGC GTTCAGTTTT 
CCTGAACTCC AGAACTTCCT GCGGATTCTC CAGCGGGAGG AAGAAGATCA CGTCCGGCAA 
ATCATACGCC GATACACTCT GGCTCGTGAT AAGATGAAAG AGGCTATGAA GAACTTCAGC 
AAGCCTGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208602.1
CDS	406..1497
Translation	MAKCELIELQDLTPNDRIELAPPSVPPPTVVPTLDRWSRGKVVRMVGERVRLEDPDWLTCKPGRGHDFQPCSQTQLSWCDLCGEFIWGLYRQSLRCTHCNYTCHYRCQPFIQLDCSSNTDTICEQSNYSEDTIETDTNVDEQSEVDWRKQDLSVTEIQQKVKEYNAQVNSNLFMVLNRDGSYTGFIKVQFKLARPVSLPPPRSVSSSSISSSCLGWDGGCQERTSFYLPRDTVKHLHISSSTRAREVIQALLNKFTVVDNPAKYSLYERSQRDNQVYLRKLADDECPLFLRLCAGPNEKVLSLVLKENETGEVNWDAFSFPELQNFLRILQREEEDHVRQIIRRYTLARDKMKEAMKNFSKPG

Target ORF information:

RefSeq Version	XM_017353113.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) domain family 1 (rassf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353113.2

ORF Insert Sequence:

ATGGCAAAAT GTGAGCTCAT CGAGTTGCAG GACTTGACTC CGAATGACCG TATTGAGCTG 
GCACCCCCTA GTGTCCCTCC ACCCACCGTG GTGCCCACTC TGGACAGGTG GAGCAGAGGG 
AAGGTGGTGC GCATGGTGGG CGAGCGCGTG CGCCTGGAGG ACCCCGATTG GCTGACGTGT 
AAACCAGGAC GAGGACATGA CTTTCAGCCC TGCAGCCAGA CACAGCTGAG CTGGTGTGAC 
CTGTGTGGAG AGTTCATCTG GGGCCTGTAC AGACAGAGCC TCCGCTGCAC ACACTGTAAC 
TACACTTGTC ACTACCGCTG TCAACCCTTC ATTCAGCTGG ACTGCAGCTC CAACACCGAC 
ACTATCTGCG AACAATCAAA CTACAGCGAG GACACCATCG AGACAGACAC CAATGTGGAT 
GAGCAGTCTG AAGTGGACTG GAGGAAACAG GATCTGTCTG TCACTGAAAT ACAGCAGAAA 
GTGAAGGAAT ACAATGCTCA GGTCAACAGT AACCTCTTCA TGGTTCTGAA TCGTGACGGC 
TCATACACTG GCTTCATCAA GGTCCAGTTT AAGCTGGCGC GACCCGTGTC TCTTCCTCCT 
CCCCGCAGCG TCTCCTCCTC CTCCATCTCC TCCTCTTGTT TAGGATGGGA TGGCGGCTGT 
CAGGAGCGAA CTTCCTTCTA CCTGCCCAGA GACACAGTCA AGCACCTGCA CATCAGCTCC 
AGCACCCGTG CCAGAGAGGT CATCCAGGCC CTGCTCAACA AGTTCACTGT GGTGGACAAT 
CCGGCTAAAT ATTCCCTGTA TGAGCGCAGC CAGCGGGACA ATCAAGTGTA CTTAAGGAAG 
TTAGCTGATG ATGAATGTCC ACTTTTCCTG CGTCTGTGTG CTGGACCCAA TGAGAAAGTC 
CTGAGTTTAG TGCTTAAAGA GAATGAAACC GGGGAAGTGA ATTGGGATGC GTTCAGTTTT 
CCTGAACTCC AGAACTTCCT GCGGATTCTC CAGCGGGAGG AAGAAGATCA CGTCCGGCAA 
ATCATACGCC GATACACTCT GGCTCGTGAT AAGATGAAAG AGGCTATGAA GAACTTCAGC 
AAGCCTGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rassf1 ( XM_017353113.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208602.1 Danio rerio Ras association (RalGDS/AF-6) domain family 1 (rassf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa26675
Clone ID Related Accession (Same CDS sequence)	XM_005173951.4 , NM_001004550.1 , XM_005173951.3 , XM_017353113.2 , XM_017353113.1
Accession Version	XM_017353113.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras association domain-containing protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAT GTGAGCTCAT CGAGTTGCAG GACTTGACTC CGAATGACCG TATTGAGCTG 
GCACCCCCTA GTGTCCCTCC ACCCACCGTG GTGCCCACTC TGGACAGGTG GAGCAGAGGG 
AAGGTGGTGC GCATGGTGGG CGAGCGCGTG CGCCTGGAGG ACCCCGATTG GCTGACGTGT 
AAACCAGGAC GAGGACATGA CTTTCAGCCC TGCAGCCAGA CACAGCTGAG CTGGTGTGAC 
CTGTGTGGAG AGTTCATCTG GGGCCTGTAC AGACAGAGCC TCCGCTGCAC ACACTGTAAC 
TACACTTGTC ACTACCGCTG TCAACCCTTC ATTCAGCTGG ACTGCAGCTC CAACACCGAC 
ACTATCTGCG AACAATCAAA CTACAGCGAG GACACCATCG AGACAGACAC CAATGTGGAT 
GAGCAGTCTG AAGTGGACTG GAGGAAACAG GATCTGTCTG TCACTGAAAT ACAGCAGAAA 
GTGAAGGAAT ACAATGCTCA GGTCAACAGT AACCTCTTCA TGGTTCTGAA TCGTGACGGC 
TCATACACTG GCTTCATCAA GGTCCAGTTT AAGCTGGCGC GACCCGTGTC TCTTCCTCCT 
CCCCGCAGCG TCTCCTCCTC CTCCATCTCC TCCTCTTGTT TAGGATGGGA TGGCGGCTGT 
CAGGAGCGAA CTTCCTTCTA CCTGCCCAGA GACACAGTCA AGCACCTGCA CATCAGCTCC 
AGCACCCGTG CCAGAGAGGT CATCCAGGCC CTGCTCAACA AGTTCACTGT GGTGGACAAT 
CCGGCTAAAT ATTCCCTGTA TGAGCGCAGC CAGCGGGACA ATCAAGTGTA CTTAAGGAAG 
TTAGCTGATG ATGAATGTCC ACTTTTCCTG CGTCTGTGTG CTGGACCCAA TGAGAAAGTC 
CTGAGTTTAG TGCTTAAAGA GAATGAAACC GGGGAAGTGA ATTGGGATGC GTTCAGTTTT 
CCTGAACTCC AGAACTTCCT GCGGATTCTC CAGCGGGAGG AAGAAGATCA CGTCCGGCAA 
ATCATACGCC GATACACTCT GGCTCGTGAT AAGATGAAAG AGGCTATGAA GAACTTCAGC 
AAGCCTGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208602.1
CDS	411..1502
Translation	MAKCELIELQDLTPNDRIELAPPSVPPPTVVPTLDRWSRGKVVRMVGERVRLEDPDWLTCKPGRGHDFQPCSQTQLSWCDLCGEFIWGLYRQSLRCTHCNYTCHYRCQPFIQLDCSSNTDTICEQSNYSEDTIETDTNVDEQSEVDWRKQDLSVTEIQQKVKEYNAQVNSNLFMVLNRDGSYTGFIKVQFKLARPVSLPPPRSVSSSSISSSCLGWDGGCQERTSFYLPRDTVKHLHISSSTRAREVIQALLNKFTVVDNPAKYSLYERSQRDNQVYLRKLADDECPLFLRLCAGPNEKVLSLVLKENETGEVNWDAFSFPELQNFLRILQREEEDHVRQIIRRYTLARDKMKEAMKNFSKPG

Target ORF information:

RefSeq Version	XM_017353113.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) domain family 1 (rassf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353113.1

ORF Insert Sequence:

ATGGCAAAAT GTGAGCTCAT CGAGTTGCAG GACTTGACTC CGAATGACCG TATTGAGCTG 
GCACCCCCTA GTGTCCCTCC ACCCACCGTG GTGCCCACTC TGGACAGGTG GAGCAGAGGG 
AAGGTGGTGC GCATGGTGGG CGAGCGCGTG CGCCTGGAGG ACCCCGATTG GCTGACGTGT 
AAACCAGGAC GAGGACATGA CTTTCAGCCC TGCAGCCAGA CACAGCTGAG CTGGTGTGAC 
CTGTGTGGAG AGTTCATCTG GGGCCTGTAC AGACAGAGCC TCCGCTGCAC ACACTGTAAC 
TACACTTGTC ACTACCGCTG TCAACCCTTC ATTCAGCTGG ACTGCAGCTC CAACACCGAC 
ACTATCTGCG AACAATCAAA CTACAGCGAG GACACCATCG AGACAGACAC CAATGTGGAT 
GAGCAGTCTG AAGTGGACTG GAGGAAACAG GATCTGTCTG TCACTGAAAT ACAGCAGAAA 
GTGAAGGAAT ACAATGCTCA GGTCAACAGT AACCTCTTCA TGGTTCTGAA TCGTGACGGC 
TCATACACTG GCTTCATCAA GGTCCAGTTT AAGCTGGCGC GACCCGTGTC TCTTCCTCCT 
CCCCGCAGCG TCTCCTCCTC CTCCATCTCC TCCTCTTGTT TAGGATGGGA TGGCGGCTGT 
CAGGAGCGAA CTTCCTTCTA CCTGCCCAGA GACACAGTCA AGCACCTGCA CATCAGCTCC 
AGCACCCGTG CCAGAGAGGT CATCCAGGCC CTGCTCAACA AGTTCACTGT GGTGGACAAT 
CCGGCTAAAT ATTCCCTGTA TGAGCGCAGC CAGCGGGACA ATCAAGTGTA CTTAAGGAAG 
TTAGCTGATG ATGAATGTCC ACTTTTCCTG CGTCTGTGTG CTGGACCCAA TGAGAAAGTC 
CTGAGTTTAG TGCTTAAAGA GAATGAAACC GGGGAAGTGA ATTGGGATGC GTTCAGTTTT 
CCTGAACTCC AGAACTTCCT GCGGATTCTC CAGCGGGAGG AAGAAGATCA CGTCCGGCAA 
ATCATACGCC GATACACTCT GGCTCGTGAT AAGATGAAAG AGGCTATGAA GAACTTCAGC 
AAGCCTGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rassf1 ( XM_005173952.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005174009.1 Danio rerio Ras association (RalGDS/AF-6) domain family 1 (rassf1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa26676
Clone ID Related Accession (Same CDS sequence)	XM_005173952.3 , XM_005173952.4
Accession Version	XM_005173952.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras association domain-containing protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173952.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG GAGCCTGCGC ACTAAATTCG ACTAGAGGAG GGCGAGGTTT AAGCGGAAAG 
GTGGGGTTTC AAATCACGCC CAATAACGGT TTCTTCCCGG TTGCACGAGG TCCGTCAGAG 
AGCCACATCA TCCCGGTCAG AAACCGCGGA ACAGAGGAAA CTCCAGTACT GTTGCGGTTT 
CGCTTGGTTT TTAGTGTGAA AATGACGGAC TGCGAGTGGT TTGACATGAC GTGGGGCAGC 
AGCGCGAGCA GCGGTTACTG CAGCCAGAGC GACTCTGACG AGCACGAGCT TGAGCTCTTC 
TATACCGCGC GCACGTCTCT GAGGAAGAGC AGAAAGTGCA GAGATAAAGA TGAGCAGTCT 
GAAGTGGACT GGAGGAAACA GGATCTGTCT GTCACTGAAA TACAGCAGAA AGTGAAGGAA 
TACAATGCTC AGGTCAACAG TAACCTCTTC ATGGTTCTGA ATCGTGACGG CTCATACACT 
GGCTTCATCA AGGTCCAGTT TAAGCTGGCG CGACCCGTGT CTCTTCCTCC TCCCCGCAGC 
GTCTCCTCCT CCTCCATCTC CTCCTCTTGT TTAGGATGGG ATGGCGGCTG TCAGGAGCGA 
ACTTCCTTCT ACCTGCCCAG AGACACAGTC AAGCACCTGC ACATCAGCTC CAGCACCCGT 
GCCAGAGAGG TCATCCAGGC CCTGCTCAAC AAGTTCACTG TGGTGGACAA TCCGGCTAAA 
TATTCCCTGT ATGAGCGCAG CCAGCGGGAC AATCAAGTGT ACTTAAGGAA GTTAGCTGAT 
GATGAATGTC CACTTTTCCT GCGTCTGTGT GCTGGACCCA ATGAGAAAGT CCTGAGTTTA 
GTGCTTAAAG AGAATGAAAC CGGGGAAGTG AATTGGGATG CGTTCAGTTT TCCTGAACTC 
CAGAACTTCC TGCGGATTCT CCAGCGGGAG GAAGAAGATC ACGTCCGGCA AATCATACGC 
CGATACACTC TGGCTCGTGA TAAGATGAAA GAGGCTATGA AGAACTTCAG CAAGCCTGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174009.1
CDS	16..1038
Translation	MEGGACALNSTRGGRGLSGKVGFQITPNNGFFPVARGPSESHIIPVRNRGTEETPVLLRFRLVFSVKMTDCEWFDMTWGSSASSGYCSQSDSDEHELELFYTARTSLRKSRKCRDKDEQSEVDWRKQDLSVTEIQQKVKEYNAQVNSNLFMVLNRDGSYTGFIKVQFKLARPVSLPPPRSVSSSSISSSCLGWDGGCQERTSFYLPRDTVKHLHISSSTRAREVIQALLNKFTVVDNPAKYSLYERSQRDNQVYLRKLADDECPLFLRLCAGPNEKVLSLVLKENETGEVNWDAFSFPELQNFLRILQREEEDHVRQIIRRYTLARDKMKEAMKNFSKPG

Target ORF information:

RefSeq Version	XM_005173952.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) domain family 1 (rassf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173952.3

ORF Insert Sequence:

ATGGAGGGCG GAGCCTGCGC ACTAAATTCG ACTAGAGGAG GGCGAGGTTT AAGCGGAAAG 
GTGGGGTTTC AAATCACGCC CAATAACGGT TTCTTCCCGG TTGCACGAGG TCCGTCAGAG 
AGCCACATCA TCCCGGTCAG AAACCGCGGA ACAGAGGAAA CTCCAGTACT GTTGCGGTTT 
CGCTTGGTTT TTAGTGTGAA AATGACGGAC TGCGAGTGGT TTGACATGAC GTGGGGCAGC 
AGCGCGAGCA GCGGTTACTG CAGCCAGAGC GACTCTGACG AGCACGAGCT TGAGCTCTTC 
TATACCGCGC GCACGTCTCT GAGGAAGAGC AGAAAGTGCA GAGATAAAGA TGAGCAGTCT 
GAAGTGGACT GGAGGAAACA GGATCTGTCT GTCACTGAAA TACAGCAGAA AGTGAAGGAA 
TACAATGCTC AGGTCAACAG TAACCTCTTC ATGGTTCTGA ATCGTGACGG CTCATACACT 
GGCTTCATCA AGGTCCAGTT TAAGCTGGCG CGACCCGTGT CTCTTCCTCC TCCCCGCAGC 
GTCTCCTCCT CCTCCATCTC CTCCTCTTGT TTAGGATGGG ATGGCGGCTG TCAGGAGCGA 
ACTTCCTTCT ACCTGCCCAG AGACACAGTC AAGCACCTGC ACATCAGCTC CAGCACCCGT 
GCCAGAGAGG TCATCCAGGC CCTGCTCAAC AAGTTCACTG TGGTGGACAA TCCGGCTAAA 
TATTCCCTGT ATGAGCGCAG CCAGCGGGAC AATCAAGTGT ACTTAAGGAA GTTAGCTGAT 
GATGAATGTC CACTTTTCCT GCGTCTGTGT GCTGGACCCA ATGAGAAAGT CCTGAGTTTA 
GTGCTTAAAG AGAATGAAAC CGGGGAAGTG AATTGGGATG CGTTCAGTTT TCCTGAACTC 
CAGAACTTCC TGCGGATTCT CCAGCGGGAG GAAGAAGATC ACGTCCGGCA AATCATACGC 
CGATACACTC TGGCTCGTGA TAAGATGAAA GAGGCTATGA AGAACTTCAG CAAGCCTGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rassf1 ( XM_005173952.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005174009.1 Danio rerio Ras association (RalGDS/AF-6) domain family 1 (rassf1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa26676
Clone ID Related Accession (Same CDS sequence)	XM_005173952.3 , XM_005173952.4
Accession Version	XM_005173952.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras association domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173952.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG GAGCCTGCGC ACTAAATTCG ACTAGAGGAG GGCGAGGTTT AAGCGGAAAG 
GTGGGGTTTC AAATCACGCC CAATAACGGT TTCTTCCCGG TTGCACGAGG TCCGTCAGAG 
AGCCACATCA TCCCGGTCAG AAACCGCGGA ACAGAGGAAA CTCCAGTACT GTTGCGGTTT 
CGCTTGGTTT TTAGTGTGAA AATGACGGAC TGCGAGTGGT TTGACATGAC GTGGGGCAGC 
AGCGCGAGCA GCGGTTACTG CAGCCAGAGC GACTCTGACG AGCACGAGCT TGAGCTCTTC 
TATACCGCGC GCACGTCTCT GAGGAAGAGC AGAAAGTGCA GAGATAAAGA TGAGCAGTCT 
GAAGTGGACT GGAGGAAACA GGATCTGTCT GTCACTGAAA TACAGCAGAA AGTGAAGGAA 
TACAATGCTC AGGTCAACAG TAACCTCTTC ATGGTTCTGA ATCGTGACGG CTCATACACT 
GGCTTCATCA AGGTCCAGTT TAAGCTGGCG CGACCCGTGT CTCTTCCTCC TCCCCGCAGC 
GTCTCCTCCT CCTCCATCTC CTCCTCTTGT TTAGGATGGG ATGGCGGCTG TCAGGAGCGA 
ACTTCCTTCT ACCTGCCCAG AGACACAGTC AAGCACCTGC ACATCAGCTC CAGCACCCGT 
GCCAGAGAGG TCATCCAGGC CCTGCTCAAC AAGTTCACTG TGGTGGACAA TCCGGCTAAA 
TATTCCCTGT ATGAGCGCAG CCAGCGGGAC AATCAAGTGT ACTTAAGGAA GTTAGCTGAT 
GATGAATGTC CACTTTTCCT GCGTCTGTGT GCTGGACCCA ATGAGAAAGT CCTGAGTTTA 
GTGCTTAAAG AGAATGAAAC CGGGGAAGTG AATTGGGATG CGTTCAGTTT TCCTGAACTC 
CAGAACTTCC TGCGGATTCT CCAGCGGGAG GAAGAAGATC ACGTCCGGCA AATCATACGC 
CGATACACTC TGGCTCGTGA TAAGATGAAA GAGGCTATGA AGAACTTCAG CAAGCCTGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174009.1
CDS	16..1038
Translation	MEGGACALNSTRGGRGLSGKVGFQITPNNGFFPVARGPSESHIIPVRNRGTEETPVLLRFRLVFSVKMTDCEWFDMTWGSSASSGYCSQSDSDEHELELFYTARTSLRKSRKCRDKDEQSEVDWRKQDLSVTEIQQKVKEYNAQVNSNLFMVLNRDGSYTGFIKVQFKLARPVSLPPPRSVSSSSISSSCLGWDGGCQERTSFYLPRDTVKHLHISSSTRAREVIQALLNKFTVVDNPAKYSLYERSQRDNQVYLRKLADDECPLFLRLCAGPNEKVLSLVLKENETGEVNWDAFSFPELQNFLRILQREEEDHVRQIIRRYTLARDKMKEAMKNFSKPG

Target ORF information:

RefSeq Version	XM_005173952.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ras association (RalGDS/AF-6) domain family 1 (rassf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173952.4

ORF Insert Sequence:

ATGGAGGGCG GAGCCTGCGC ACTAAATTCG ACTAGAGGAG GGCGAGGTTT AAGCGGAAAG 
GTGGGGTTTC AAATCACGCC CAATAACGGT TTCTTCCCGG TTGCACGAGG TCCGTCAGAG 
AGCCACATCA TCCCGGTCAG AAACCGCGGA ACAGAGGAAA CTCCAGTACT GTTGCGGTTT 
CGCTTGGTTT TTAGTGTGAA AATGACGGAC TGCGAGTGGT TTGACATGAC GTGGGGCAGC 
AGCGCGAGCA GCGGTTACTG CAGCCAGAGC GACTCTGACG AGCACGAGCT TGAGCTCTTC 
TATACCGCGC GCACGTCTCT GAGGAAGAGC AGAAAGTGCA GAGATAAAGA TGAGCAGTCT 
GAAGTGGACT GGAGGAAACA GGATCTGTCT GTCACTGAAA TACAGCAGAA AGTGAAGGAA 
TACAATGCTC AGGTCAACAG TAACCTCTTC ATGGTTCTGA ATCGTGACGG CTCATACACT 
GGCTTCATCA AGGTCCAGTT TAAGCTGGCG CGACCCGTGT CTCTTCCTCC TCCCCGCAGC 
GTCTCCTCCT CCTCCATCTC CTCCTCTTGT TTAGGATGGG ATGGCGGCTG TCAGGAGCGA 
ACTTCCTTCT ACCTGCCCAG AGACACAGTC AAGCACCTGC ACATCAGCTC CAGCACCCGT 
GCCAGAGAGG TCATCCAGGC CCTGCTCAAC AAGTTCACTG TGGTGGACAA TCCGGCTAAA 
TATTCCCTGT ATGAGCGCAG CCAGCGGGAC AATCAAGTGT ACTTAAGGAA GTTAGCTGAT 
GATGAATGTC CACTTTTCCT GCGTCTGTGT GCTGGACCCA ATGAGAAAGT CCTGAGTTTA 
GTGCTTAAAG AGAATGAAAC CGGGGAAGTG AATTGGGATG CGTTCAGTTT TCCTGAACTC 
CAGAACTTCC TGCGGATTCT CCAGCGGGAG GAAGAAGATC ACGTCCGGCA AATCATACGC 
CGATACACTC TGGCTCGTGA TAAGATGAAA GAGGCTATGA AGAACTTCAG CAAGCCTGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Global and gene specific DNA methylation changes during zebrafish development.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology166(1)99-108(2013 Sep)
Fang X,Corrales J,Thornton C,Scheffler BE,Willett KL

Benzo[a]pyrene decreases global and gene specific DNA methylation during zebrafish development.
Environmental toxicology and pharmacology36(1)40-50(2013 Jul)
Fang X,Thornton C,Scheffler BE,Willett KL

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rassf1 cDNA ORF clone, Danio rerio(zebrafish)

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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