kctd15b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	kctd15b
Entrez Gene ID	445133
Full Name	potassium channel tetramerization domain containing 15b
Synonyms	kctd15,zgc:100865
General protein information	Preferred Names potassium channel tetramerization domain containing 15b Names BTB/POZ domain-containing protein kctd15 novel potassium channel tetramerisation domain protein potassium channel tetramerisation domain containing 15 potassium channel tetramerisation domain containing 15b potassium channel tetramerization domain-containing protein 15
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003527.1	NP_001003527.1	BTB/POZ domain-containing protein kctd15

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	KCTD15	XP_001154150.1
Drosophila melanogaster (fruit fly)	CG10440	NP_001246449.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007044	XP_308719.3
Homo sapiens (human)	KCTD15	NP_001123467.1
Bos taurus (cattle)	KCTD15	NP_001069036.1
Mus musculus (house mouse)	Kctd15	NP_666300.1
	KCTD15	XP_001089394.1
Canis lupus familiaris (dog)	KCTD15	XP_541709.2
Rattus norvegicus (Norway rat)	Kctd15	NP_001102611.1
Gallus gallus (chicken)	KCTD15	XP_414140.4
Danio rerio (zebrafish)	kctd15b	NP_001003527.1
Xenopus tropicalis (tropical clawed frog)	kctd15	NP_989239.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Generation and characterization of Kctd15 mutations in zebrafish.
Heffer A, Marquart GD, Aquilina-Beck A, Saleem N, Burgess HA, Dawid IB
PloS one12(12)e0189162(2017)

The BTB-containing protein Kctd15 is SUMOylated in vivo.
Zarelli VE, Dawid IB
PloS one8(9)e75016(2013)

Zebrafish enhancer trap line showing maternal and neural expression of kctd15a.
Gharbi N, Zhao XF, Ellingsen S, Fjose A
Development, growth & differentiation54(2)241-52(2012 Feb)

Kctd15 inhibits neural crest formation by attenuating Wnt/beta-catenin signaling output.
Dutta S, Dawid IB
Development (Cambridge, England)137(18)3013-8(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

kctd15 mutants show deficits in the development of both neural crest derivatives, and specific regions within the central nervous system, leading to a strong reduction in normal growth rates.
Title: Generation and characterization of Kctd15 mutations in zebrafish.

These data suggest that the non-SUMOylated form of Kctd15 functions in neural crest development.
Title: The BTB-containing protein Kctd15 is SUMOylated in vivo.

Suppression of neural crest induction by inhibition of Wnt8.1 was rescued by reduction of Kctd15 expression, linking Kctd15 action to the Wnt pathway.
Title: Kctd15 inhibits neural crest formation by attenuating Wnt/beta-catenin signaling output.

The following kctd15b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kctd15b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38533	NM_001003527.1 Latest version!	Danio rerio potassium channel tetramerization domain containing 15b (kctd15b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kctd15b ( NM_001003527.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003527.1 Danio rerio potassium channel tetramerization domain containing 15b (kctd15b), mRNA.

Price & Availability↑

CloneID	ODa38533
Clone ID Related Accession (Same CDS sequence)	NM_001003527.1
Accession Version	NM_001003527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	BTB/POZ domain-containing protein kctd15
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC078294.1. On Jul 6, 2005 this sequence version replaced XM_696334.1. ##Evidence-Data-START## Transcript exon combination :: BC078294.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168447, SAMEA3505373 [ECO:0000350] ##Evidence-Data-END##

ATGTCGCGCC TGTCTCTGAC CCGGTCTCCG GTCTCTCCTC TGGCCGCGCA GGGGATTCCT 
CTGCCGGCGC AGCTCACCAA AGCCAACGCG CCCGTGCACA TCGACGTGGG CGGACACATG 
TACACCAGCA GCCTGGCCAC GCTCACCAAA TACCCAGACT CACGGATCAG CAGGCTGTTT 
AACGGGACGG AGCCCATCGT TCTGGACAGT TTAAAGCAGC ATTATTTCAT CGACAGAGAT 
GGAGAAATCT TCCGCTATAT TCTGAGTTTC CTGCGAACCT GCAAACTGCT GCTGCCCGAC 
GACTTTAAGG ACTTCAATCT GCTGTACGAG GAGGCGCGCT ATTACCAGCT GAGTCCGATG 
ATCAAGGAGC TGGAGCGCTG GAAGCAGGAG CGTGAGCAGC GGCGTCTGGC TAACCCCTGC 
GACTGCCTGG TGGTGCGGGT GACCCCCGAC CTGGGCGAAC GCATCGCTCT GAGCGGAGAG 
AAGGTGCTCA TCGAGGAGAT CTTCCCGGAG ACCGGAGACG TCATGTGCAA CTCGGTCAAC 
GCTGGCTGGA ACCAGGACCC CACACACGTC ATCCGCTTCC CACTGAACGG ATACTGCAGA 
CTCAACTCCG TGCAGGTGCT GGAGCGTCTC TTCCAGAAGG GTTTCAATGT GGCGGCGTCA 
TGTGGTGGTG GCGTGGACTC GTCTCAGTTC AGCGAATATG TTCTGTGTCG TGAAGATCGG 
CGGAGTCACA ACACAAACAC ACCCATCAGG ATAAAGCAGG AGCCGCTGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003527.1
CDS	71..844
Translation	MSRLSLTRSPVSPLAAQGIPLPAQLTKANAPVHIDVGGHMYTSSLATLTKYPDSRISRLFNGTEPIVLDSLKQHYFIDRDGEIFRYILSFLRTCKLLLPDDFKDFNLLYEEARYYQLSPMIKELERWKQEREQRRLANPCDCLVVRVTPDLGERIALSGEKVLIEEIFPETGDVMCNSVNAGWNQDPTHVIRFPLNGYCRLNSVQVLERLFQKGFNVAASCGGGVDSSQFSEYVLCREDRRSHNTNTPIRIKQEPLD

Target ORF information:

RefSeq Version	NM_001003527.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio potassium channel tetramerization domain containing 15b (kctd15b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003527.1

ORF Insert Sequence:

ATGTCGCGCC TGTCTCTGAC CCGGTCTCCG GTCTCTCCTC TGGCCGCGCA GGGGATTCCT 
CTGCCGGCGC AGCTCACCAA AGCCAACGCG CCCGTGCACA TCGACGTGGG CGGACACATG 
TACACCAGCA GCCTGGCCAC GCTCACCAAA TACCCAGACT CACGGATCAG CAGGCTGTTT 
AACGGGACGG AGCCCATCGT TCTGGACAGT TTAAAGCAGC ATTATTTCAT CGACAGAGAT 
GGAGAAATCT TCCGCTATAT TCTGAGTTTC CTGCGAACCT GCAAACTGCT GCTGCCCGAC 
GACTTTAAGG ACTTCAATCT GCTGTACGAG GAGGCGCGCT ATTACCAGCT GAGTCCGATG 
ATCAAGGAGC TGGAGCGCTG GAAGCAGGAG CGTGAGCAGC GGCGTCTGGC TAACCCCTGC 
GACTGCCTGG TGGTGCGGGT GACCCCCGAC CTGGGCGAAC GCATCGCTCT GAGCGGAGAG 
AAGGTGCTCA TCGAGGAGAT CTTCCCGGAG ACCGGAGACG TCATGTGCAA CTCGGTCAAC 
GCTGGCTGGA ACCAGGACCC CACACACGTC ATCCGCTTCC CACTGAACGG ATACTGCAGA 
CTCAACTCCG TGCAGGTGCT GGAGCGTCTC TTCCAGAAGG GTTTCAATGT GGCGGCGTCA 
TGTGGTGGTG GCGTGGACTC GTCTCAGTTC AGCGAATATG TTCTGTGTCG TGAAGATCGG 
CGGAGTCACA ACACAAACAC ACCCATCAGG ATAAAGCAGG AGCCGCTGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Generation and characterization of Kctd15 mutations in zebrafish.
PloS one12(12)e0189162(2017)
Heffer A,Marquart GD,Aquilina-Beck A,Saleem N,Burgess HA,Dawid IB

The BTB-containing protein Kctd15 is SUMOylated in vivo.
PloS one8(9)e75016(2013)
Zarelli VE,Dawid IB

Zebrafish enhancer trap line showing maternal and neural expression of kctd15a.
Development, growth & differentiation54(2)241-52(2012 Feb)
Gharbi N,Zhao XF,Ellingsen S,Fjose A

Kctd15 inhibits neural crest formation by attenuating Wnt/beta-catenin signaling output.
Development (Cambridge, England)137(18)3013-8(2010 Sep)
Dutta S,Dawid IB