asic4b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	asic4b
Entrez Gene ID	407667
Full Name	acid-sensing (proton-gated) ion channel family member 4b
Synonyms	ASIC4,accn4b,zASIC4.2,zgc:111839
General protein information	Preferred Names acid-sensing (proton-gated) ion channel family member 4b Names acid-sensing ion channel 4 acid-sensing ion channel 4.2 amiloride-sensitive cation channel 4 b amiloride-sensitive cation channel 4-B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_214786.1	NP_999951.1	acid-sensing ion channel 4-B

Pathways from BioSystems

Homologies

Homo sapiens (human)	ASIC4	NP_878267.2
	LOC703972	XP_002808071.1
Rattus norvegicus (Norway rat)	Asic4	NP_071570.2
Pan troglodytes (chimpanzee)	ACCN4	XP_001163732.2
Mus musculus (house mouse)	Asic4	NP_898843.1
Danio rerio (zebrafish)	asic4b	NP_999951.1
Bos taurus (cattle)	ASIC4	XP_003585817.2
Xenopus tropicalis (tropical clawed frog)	asic4	XP_002934008.2
Canis lupus familiaris (dog)	ASIC4	XP_005640766.1
Gallus gallus (chicken)	ASIC4	XP_004942894.1
Danio rerio (zebrafish)	asic4a	NP_999952.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptomic, proteomic, and metabolomic landscape of positional memory in the caudal fin of zebrafish.
Rabinowitz JS, Robitaille AM, Wang Y, Ray CA, Thummel R, Gu H, Djukovic D, Raftery D, Berndt JD, Moon RT
Proceedings of the National Academy of Sciences of the United States of America114(5)E717-E726(2017 Jan)

Fine selection of up-regulated genes during ovulation by
Klangnurak W, Tokumoto T
Zoological letters32(2017)

Acid-sensing ion channels (ASICs) 2 and 4.2 are expressed in the retina of the adult zebrafish.
Vi?a E, Parisi V, S?nchez-Ramos C, Cabo R, Guerrera MC, Quir?s LM, German? A, Vega JA, Garc?a-Su?rez O
Cell and tissue research360(2)223-31(2015 May)

The role of acid-sensing ion channels in epithelial Na+ uptake in adult zebrafish (Danio rerio).
Dymowska AK, Boyle D, Schultz AG, Goss GG
The Journal of experimental biology218(Pt 8)1244-51(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results are the first demonstration that ASIC2 and ASIC4 are expressed in the adult zebrafish retina
Title: Acid-sensing ion channels (ASICs) 2 and 4.2 are expressed in the retina of the adult zebrafish.

zASIC4.1 is gated by extracellular H+, zASIC4.2 is not.
Title: Zebrafish acid-sensing ion channel (ASIC) 4, characterization of homo- and heteromeric channels, and identification of regions important for activation by H+.

The 6 zasic genes studied are broadly expressed in the central nervous system, whereas expression in the peripheral nervous system is scarce. zASICs are activated by extracellular H(+), Na(+) selectivity and blocked by amiloride.
Title: A family of acid-sensing ion channels from the zebrafish: widespread expression in the central nervous system suggests a conserved role in neuronal communication.

The following asic4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the asic4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38023	NM_214786.1 Latest version!	Danio rerio acid-sensing (proton-gated) ion channel family member 4b (asic4b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

asic4b ( NM_214786.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_999951.1 Danio rerio acid-sensing (proton-gated) ion channel family member 4b (asic4b), mRNA.

Price & Availability↑

CloneID	ODa38023
Clone ID Related Accession (Same CDS sequence)	NM_214786.1
Accession Version	NM_214786.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Danio rerio(zebrafish)
Product	acid-sensing ion channel 4-B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ609620.1. ##Evidence-Data-START## Transcript exon combination :: AJ609620.1, BC095568.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476744, SAMEA4476749 [ECO:0000348] ##Evidence-Data-END##

ATGCCCATCG AATTTGTTTG TAAAATCAAG TTCGCTGAGG GCGAAGAAGC CAAGGGGGCC 
TCGACAGAAG GAGGAGGAAC CGGCATGCTA GATGAAGGCC TTCGGCGGCA AAAAGAAGGC 
ATGGCTGACC TGGCATCCTT TGCAAGCTCC TCTTCACTTC ACGGTTTGGC TCGGGCACTA 
GGGACCTCGG AGCGCTTGGG TTTCAGACAG ACTCTTTGGG GACTGGCACT GCTGGTCTCG 
CTTGGTCTCT TCCTGTACCA GGCGACATGG AGTGCGGCGA CTTATCTGGA GCGTCCCCAC 
CTCGCAGCTC TAAGGGAAGA AACCCGCCGA GAGCTCACCT TTCCGGCCAT CACGCTCTGT 
AACGTCAACA GATTCCGCTT CTCTGCCCTC ACAGATGCGG ACATCTACCA CTTGGCTAAC 
CTCACCGGCC TTCCGCCCAA GAGCCGTAAA GGACACCGGC CAAGCGAGCT CCAGTACCCA 
CCCCCAAACA TGCTAGACAT TTTCCAGAGG ACTGGCCACC AGCTGGAAGA CATGCTGAAG 
AGCTGCAACT TCAGCGGGCA GAACTGCTCA AGCGAAGACT TCAGCGTGGT TTACACACGG 
TATGGGAAAT GTTACACATT TAATGGAAAC AAGACGTCAC CCAAGCGTGT AAGGCAAGGT 
GGCACAGGAA ACGGCCTGGA GATGATGCTG GATATTCAGC AGGACGAGTA TCTGCCTATC 
TGGAGAGAAA CCAACGAGAC TACGCTGGAA GCAGGTATTC GGGTTCAGAT ACACAGTCAG 
AATGAACCCC CGTACATCCA CCAGCTGGGC TTTGGAGTCT CACCAGGATT CCAAACATTT 
GTTTCCTGCC AGGAACAGAG GCTAACATAC CTGCCACAGC CGTGGGGAAA CTGTCGGGCT 
TCATCCGAGC CTGTGATCCC AGGATACGAC ACCTACAGCG TCAGCGCCTG CAGACTGCAC 
TGTGAGAGCA CACAGGTGCA GAGAGAGTGC AACTGCAGGA TGGTGCATAT GCCAGGTGAT 
GCTGATATCT GTGCACCCAG TAAAATCAAG TGTGTTGACA AGGCTTTGGC TTCACTCCAG 
AAGAGCACAG GTGACTCATG TCCCTGCGAG ACACCCTGTA ATTTGACCCG GTATGGGAAA 
GAGCTCTCTA TGGTTAAAAT CCCCAGCAGA GGCTCTGCTC GCTACCTTTC TCGCAAGTAC 
CAGAAGTCAG AGGAGTACAT CAGAGACAAC TTTCTCATCT TGGATATTTT CTTTGAGGCC 
CTTAACTATG AAACGATTGA GCAGAAGAAA GCGTACGACA TCGCTGGTTT GTTAGGAGAC 
ATTGGTGGAC AGATGGGCCT CTTCATTGGA GCCAGTATTC TTACCATACT GGAAATACTC 
GACTACATCT ATGAGGTAGC CAAAAACAAA ATCAAGCAAC TCCTGAAACC AAAGAAGAGC 
CAGAAACAAA CCAACCAACG GAACTTGATC CAAGAGCAGA TCCAGAGAAC CAAGAACCTG 
CGGGAGCAGA ACCTGAAAGC TCAGCTAACT GCCGGAGCAA TAGCTACAGT CAGGTTTGAA 
GAAGTCAAAG TCAAGGCAGC TAATGATGTG GCTCAACCAC ACAGCGCACA TCCAACTTCG 
GTATTACCAA ATCATCACAA TGCACAACAA GCCGTACAGC AGGACTTTGC TTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999951.1
CDS	317..1993
Translation	MPIEFVCKIKFAEGEEAKGASTEGGGTGMLDEGLRRQKEGMADLASFASSSSLHGLARALGTSERLGFRQTLWGLALLVSLGLFLYQATWSAATYLERPHLAALREETRRELTFPAITLCNVNRFRFSALTDADIYHLANLTGLPPKSRKGHRPSELQYPPPNMLDIFQRTGHQLEDMLKSCNFSGQNCSSEDFSVVYTRYGKCYTFNGNKTSPKRVRQGGTGNGLEMMLDIQQDEYLPIWRETNETTLEAGIRVQIHSQNEPPYIHQLGFGVSPGFQTFVSCQEQRLTYLPQPWGNCRASSEPVIPGYDTYSVSACRLHCESTQVQRECNCRMVHMPGDADICAPSKIKCVDKALASLQKSTGDSCPCETPCNLTRYGKELSMVKIPSRGSARYLSRKYQKSEEYIRDNFLILDIFFEALNYETIEQKKAYDIAGLLGDIGGQMGLFIGASILTILEILDYIYEVAKNKIKQLLKPKKSQKQTNQRNLIQEQIQRTKNLREQNLKAQLTAGAIATVRFEEVKVKAANDVAQPHSAHPTSVLPNHHNAQQAVQQDFAC

Target ORF information:

RefSeq Version	NM_214786.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acid-sensing (proton-gated) ion channel family member 4b (asic4b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214786.1

ORF Insert Sequence:

ATGCCCATCG AATTTGTTTG TAAAATCAAG TTCGCTGAGG GCGAAGAAGC CAAGGGGGCC 
TCGACAGAAG GAGGAGGAAC CGGCATGCTA GATGAAGGCC TTCGGCGGCA AAAAGAAGGC 
ATGGCTGACC TGGCATCCTT TGCAAGCTCC TCTTCACTTC ACGGTTTGGC TCGGGCACTA 
GGGACCTCGG AGCGCTTGGG TTTCAGACAG ACTCTTTGGG GACTGGCACT GCTGGTCTCG 
CTTGGTCTCT TCCTGTACCA GGCGACATGG AGTGCGGCGA CTTATCTGGA GCGTCCCCAC 
CTCGCAGCTC TAAGGGAAGA AACCCGCCGA GAGCTCACCT TTCCGGCCAT CACGCTCTGT 
AACGTCAACA GATTCCGCTT CTCTGCCCTC ACAGATGCGG ACATCTACCA CTTGGCTAAC 
CTCACCGGCC TTCCGCCCAA GAGCCGTAAA GGACACCGGC CAAGCGAGCT CCAGTACCCA 
CCCCCAAACA TGCTAGACAT TTTCCAGAGG ACTGGCCACC AGCTGGAAGA CATGCTGAAG 
AGCTGCAACT TCAGCGGGCA GAACTGCTCA AGCGAAGACT TCAGCGTGGT TTACACACGG 
TATGGGAAAT GTTACACATT TAATGGAAAC AAGACGTCAC CCAAGCGTGT AAGGCAAGGT 
GGCACAGGAA ACGGCCTGGA GATGATGCTG GATATTCAGC AGGACGAGTA TCTGCCTATC 
TGGAGAGAAA CCAACGAGAC TACGCTGGAA GCAGGTATTC GGGTTCAGAT ACACAGTCAG 
AATGAACCCC CGTACATCCA CCAGCTGGGC TTTGGAGTCT CACCAGGATT CCAAACATTT 
GTTTCCTGCC AGGAACAGAG GCTAACATAC CTGCCACAGC CGTGGGGAAA CTGTCGGGCT 
TCATCCGAGC CTGTGATCCC AGGATACGAC ACCTACAGCG TCAGCGCCTG CAGACTGCAC 
TGTGAGAGCA CACAGGTGCA GAGAGAGTGC AACTGCAGGA TGGTGCATAT GCCAGGTGAT 
GCTGATATCT GTGCACCCAG TAAAATCAAG TGTGTTGACA AGGCTTTGGC TTCACTCCAG 
AAGAGCACAG GTGACTCATG TCCCTGCGAG ACACCCTGTA ATTTGACCCG GTATGGGAAA 
GAGCTCTCTA TGGTTAAAAT CCCCAGCAGA GGCTCTGCTC GCTACCTTTC TCGCAAGTAC 
CAGAAGTCAG AGGAGTACAT CAGAGACAAC TTTCTCATCT TGGATATTTT CTTTGAGGCC 
CTTAACTATG AAACGATTGA GCAGAAGAAA GCGTACGACA TCGCTGGTTT GTTAGGAGAC 
ATTGGTGGAC AGATGGGCCT CTTCATTGGA GCCAGTATTC TTACCATACT GGAAATACTC 
GACTACATCT ATGAGGTAGC CAAAAACAAA ATCAAGCAAC TCCTGAAACC AAAGAAGAGC 
CAGAAACAAA CCAACCAACG GAACTTGATC CAAGAGCAGA TCCAGAGAAC CAAGAACCTG 
CGGGAGCAGA ACCTGAAAGC TCAGCTAACT GCCGGAGCAA TAGCTACAGT CAGGTTTGAA 
GAAGTCAAAG TCAAGGCAGC TAATGATGTG GCTCAACCAC ACAGCGCACA TCCAACTTCG 
GTATTACCAA ATCATCACAA TGCACAACAA GCCGTACAGC AGGACTTTGC TTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptomic, proteomic, and metabolomic landscape of positional memory in the caudal fin of zebrafish.
Proceedings of the National Academy of Sciences of the United States of America114(5)E717-E726(2017 Jan)
Rabinowitz JS,Robitaille AM,Wang Y,Ray CA,Thummel R,Gu H,Djukovic D,Raftery D,Berndt JD,Moon RT

Fine selection of up-regulated genes during ovulation by
Zoological letters32(2017)
Klangnurak W,Tokumoto T

Acid-sensing ion channels (ASICs) 2 and 4.2 are expressed in the retina of the adult zebrafish.
Cell and tissue research360(2)223-31(2015 May)
Vi?a E,Parisi V,S?nchez-Ramos C,Cabo R,Guerrera MC,Quir?s LM,German? A,Vega JA,Garc?a-Su?rez O

The role of acid-sensing ion channels in epithelial Na+ uptake in adult zebrafish (Danio rerio).
The Journal of experimental biology218(Pt 8)1244-51(2015 Apr)
Dymowska AK,Boyle D,Schultz AG,Goss GG