Asic4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Asic4
Entrez Gene ID	241118
Full Name	acid-sensing (proton-gated) ion channel family member 4
Synonyms	Accn4,BNAC4
General protein information	Preferred Names acid-sensing (proton-gated) ion channel family member 4 Names acid-sensing ion channel 4 amiloride-sensitive cation channel 4, pituitary
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_183022.3	NP_898843.1	acid-sensing ion channel 4

Pathways from BioSystems

Homologies

Homo sapiens (human)	ASIC4	NP_878267.2
	LOC703972	XP_002808071.1
Rattus norvegicus (Norway rat)	Asic4	NP_071570.2
Pan troglodytes (chimpanzee)	ACCN4	XP_001163732.2
Mus musculus (house mouse)	Asic4	NP_898843.1
Danio rerio (zebrafish)	asic4b	NP_999951.1
Bos taurus (cattle)	ASIC4	XP_003585817.2
Xenopus tropicalis (tropical clawed frog)	asic4	XP_002934008.2
Canis lupus familiaris (dog)	ASIC4	XP_005640766.1
Gallus gallus (chicken)	ASIC4	XP_004942894.1
Danio rerio (zebrafish)	asic4a	NP_999952.1

Gene Ontology
Bibliography

Related articles in PubMed

Distribution of ASIC4 transcripts in the adult wild-type mouse brain.
Hoshikawa M, Kato A, Hojo H, Shibata Y, Kumamoto N, Watanabe M, Ugawa S
Neuroscience letters65157-64(2017 Jun)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Genetic mapping of ASIC4 and contrasting phenotype to ASIC1a in modulating innate fear and anxiety.
Lin SH, Chien YC, Chiang WW, Liu YZ, Lien CC, Chen CC
The European journal of neuroscience41(12)1553-68(2015 Jun)

Developmental waves of mechanosensitivity acquisition in sensory neuron subtypes during embryonic development.
Lechner SG, Frenzel H, Wang R, Lewin GR
The EMBO journal28(10)1479-91(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonstrated that ASIC4 may modulate fear and anxiety by counteracting ASIC1a activity in the brain.
Title: Genetic mapping of ASIC4 and contrasting phenotype to ASIC1a in modulating innate fear and anxiety.

The following Asic4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Asic4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08304	NM_183022.3 Latest version!	Mus musculus acid-sensing (proton-gated) ion channel family member 4 (Asic4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Asic4 ( NM_183022.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_898843.1 Mus musculus acid-sensing (proton-gated) ion channel family member 4 (Asic4), mRNA.

Price & Availability↑

CloneID	OMu08304
Clone ID Related Accession (Same CDS sequence)	NM_183022.3
Accession Version	NM_183022.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Mus musculus(house mouse)
Product	acid-sensing ion channel 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB621654.1, BC055772.1 and BY652580.1. On Apr 26, 2007 this sequence version replaced NM_183022.2. ##Evidence-Data-START## Transcript exon combination :: BC055772.1, BC046481.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END##

ATGCCGATCG AGATTGTGTG CAAAATCAAA TTTGCTGAGG AGGATGCAAA ACCCAAGGAG 
AAGGAGGCAG GGGATGAACA GAGCCTCCTG GGGGCTGCTC AGGGGCCAGC AGCCCCTCGG 
GACCTGGCCA CCTTTGCCAG CACCAGCACT CTGCATGGGC TGGGCCGGGC CTGTGGCCCT 
GGCCCCCATG GACTGCGCAG AACCCTGTGG GCACTGGCCC TACTCACCTC ACTGGCTGCC 
TTTCTGTACC AGGCAGCCAG CCTGGCCAGG GGCTACCTGA CCCGGCCTCA CCTGGTAGCC 
ATGGACCCTG CTGCCCCAGC CCCAGTGGCG GGTTTTCCGG CTGTCACCCT CTGCAACATC 
AACCGCTTCC GGCACTCGGC ACTCAGCGAT GCTGATATCT TCCACCTGGC CAATCTGACA 
GGGCTGCCCC CCAAAGACCG GGACGGGCAC CGTGCAGCTG GCCTTCGCTA TCCAGAGCCT 
GACATGGTAG ACATCCTCAA CCGCACTGGC CACCAGCTTG CTGACATGCT CAAGAGCTGC 
AACTTCAGTG GGCACCACTG CTCCGCCAGC AACTTCTCTG TGGTCTATAC TCGCTATGGG 
AAGTGTTACA CCTTTAATGC AGATCCTCAG AGTTCACTGC CCAGTAGGGC AGGCGGCATG 
GGTAGTGGCC TGGAGATCAT GCTAGACATC CAGCAGGAGG AATACCTACC CATATGGAGG 
GAGACAAACG AGACGTCATT CGAGGCAGGG ATCCGGGTGC AGATCCACAG CCAGGAGGAG 
CCGCCCTACA TCCACCAGCT GGGGTTTGGC GTGTCCCCAG GCTTCCAGAC TTTTGTGTCC 
TGCCAGGAAC AGCGGCTAAC TTATCTGCCC CAGCCTTGGG GCAACTGCCG GGCGGAAAGC 
GAGCTCAGGG AGCCTGAGCT TCAGGGCTAC TCAGCCTATA GTGTGTCTGC CTGCCGACTG 
CGCTGTGAGA AGGAGGCCGT GCTTCAGCGC TGCCACTGCC GGATGGTGCA CATGCCAGGC 
AACGAGACCA TCTGCCCACC AAATATCTAC ATTGAATGTG CCGACCACAC ACTGGACTCC 
CTTGGTGGGG GCTCCGAGGG CCCATGCTTC TGCCCTACAC CCTGCAACCT GACTCGTTAC 
GGCAAAGAGA TCTCCATGGT CAAGATCCCC AACAGGGGCT CAGCCAGATA CCTGGCAAGG 
AAGTACAACC GCAATGAGAC CTATATAAGG GAGAACTTCT TGGTCCTGGA TGTCTTTTTC 
GAAGCCCTAA CTTCTGAAGC CATGGAACAG CAAGCCGCCT ATGGCCTGTC AGCCCTGCTG 
GGGGACCTTG GGGGACAGAT GGGCCTGTTC ATTGGGGCTA GCATCCTCAC CTTGCTGGAG 
ATCCTTGACT ACATCTATGA GGTCTCCTGG GATCGGCTCA AGAGGGTGTG GCGACGGCCC 
AAGACCCCAC TGCGGACATC CACCGGGGGC ATCTCCACTC TGGGGCTGCA GGAGCTGAAG 
GAACAGAGTC CCTGTCCAAG CCGGGGCCGT GCTGAGGGTG GGGGGGCTAG CAGCCTGCTT 
CCCAACCATC ACCACCCTCA CGGCCCCCCA GGAAGCCTCT TTGAAGACTT TGCTTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_898843.1
CDS	323..1942
Translation	MPIEIVCKIKFAEEDAKPKEKEAGDEQSLLGAAQGPAAPRDLATFASTSTLHGLGRACGPGPHGLRRTLWALALLTSLAAFLYQAASLARGYLTRPHLVAMDPAAPAPVAGFPAVTLCNINRFRHSALSDADIFHLANLTGLPPKDRDGHRAAGLRYPEPDMVDILNRTGHQLADMLKSCNFSGHHCSASNFSVVYTRYGKCYTFNADPQSSLPSRAGGMGSGLEIMLDIQQEEYLPIWRETNETSFEAGIRVQIHSQEEPPYIHQLGFGVSPGFQTFVSCQEQRLTYLPQPWGNCRAESELREPELQGYSAYSVSACRLRCEKEAVLQRCHCRMVHMPGNETICPPNIYIECADHTLDSLGGGSEGPCFCPTPCNLTRYGKEISMVKIPNRGSARYLARKYNRNETYIRENFLVLDVFFEALTSEAMEQQAAYGLSALLGDLGGQMGLFIGASILTLLEILDYIYEVSWDRLKRVWRRPKTPLRTSTGGISTLGLQELKEQSPCPSRGRAEGGGASSLLPNHHHPHGPPGSLFEDFAC

Target ORF information:

RefSeq Version	NM_183022.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus acid-sensing (proton-gated) ion channel family member 4 (Asic4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183022.3

ORF Insert Sequence:

ATGCCGATCG AGATTGTGTG CAAAATCAAA TTTGCTGAGG AGGATGCAAA ACCCAAGGAG 
AAGGAGGCAG GGGATGAACA GAGCCTCCTG GGGGCTGCTC AGGGGCCAGC AGCCCCTCGG 
GACCTGGCCA CCTTTGCCAG CACCAGCACT CTGCATGGGC TGGGCCGGGC CTGTGGCCCT 
GGCCCCCATG GACTGCGCAG AACCCTGTGG GCACTGGCCC TACTCACCTC ACTGGCTGCC 
TTTCTGTACC AGGCAGCCAG CCTGGCCAGG GGCTACCTGA CCCGGCCTCA CCTGGTAGCC 
ATGGACCCTG CTGCCCCAGC CCCAGTGGCG GGTTTTCCGG CTGTCACCCT CTGCAACATC 
AACCGCTTCC GGCACTCGGC ACTCAGCGAT GCTGATATCT TCCACCTGGC CAATCTGACA 
GGGCTGCCCC CCAAAGACCG GGACGGGCAC CGTGCAGCTG GCCTTCGCTA TCCAGAGCCT 
GACATGGTAG ACATCCTCAA CCGCACTGGC CACCAGCTTG CTGACATGCT CAAGAGCTGC 
AACTTCAGTG GGCACCACTG CTCCGCCAGC AACTTCTCTG TGGTCTATAC TCGCTATGGG 
AAGTGTTACA CCTTTAATGC AGATCCTCAG AGTTCACTGC CCAGTAGGGC AGGCGGCATG 
GGTAGTGGCC TGGAGATCAT GCTAGACATC CAGCAGGAGG AATACCTACC CATATGGAGG 
GAGACAAACG AGACGTCATT CGAGGCAGGG ATCCGGGTGC AGATCCACAG CCAGGAGGAG 
CCGCCCTACA TCCACCAGCT GGGGTTTGGC GTGTCCCCAG GCTTCCAGAC TTTTGTGTCC 
TGCCAGGAAC AGCGGCTAAC TTATCTGCCC CAGCCTTGGG GCAACTGCCG GGCGGAAAGC 
GAGCTCAGGG AGCCTGAGCT TCAGGGCTAC TCAGCCTATA GTGTGTCTGC CTGCCGACTG 
CGCTGTGAGA AGGAGGCCGT GCTTCAGCGC TGCCACTGCC GGATGGTGCA CATGCCAGGC 
AACGAGACCA TCTGCCCACC AAATATCTAC ATTGAATGTG CCGACCACAC ACTGGACTCC 
CTTGGTGGGG GCTCCGAGGG CCCATGCTTC TGCCCTACAC CCTGCAACCT GACTCGTTAC 
GGCAAAGAGA TCTCCATGGT CAAGATCCCC AACAGGGGCT CAGCCAGATA CCTGGCAAGG 
AAGTACAACC GCAATGAGAC CTATATAAGG GAGAACTTCT TGGTCCTGGA TGTCTTTTTC 
GAAGCCCTAA CTTCTGAAGC CATGGAACAG CAAGCCGCCT ATGGCCTGTC AGCCCTGCTG 
GGGGACCTTG GGGGACAGAT GGGCCTGTTC ATTGGGGCTA GCATCCTCAC CTTGCTGGAG 
ATCCTTGACT ACATCTATGA GGTCTCCTGG GATCGGCTCA AGAGGGTGTG GCGACGGCCC 
AAGACCCCAC TGCGGACATC CACCGGGGGC ATCTCCACTC TGGGGCTGCA GGAGCTGAAG 
GAACAGAGTC CCTGTCCAAG CCGGGGCCGT GCTGAGGGTG GGGGGGCTAG CAGCCTGCTT 
CCCAACCATC ACCACCCTCA CGGCCCCCCA GGAAGCCTCT TTGAAGACTT TGCTTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Distribution of ASIC4 transcripts in the adult wild-type mouse brain.
Neuroscience letters65157-64(2017 Jun)
Hoshikawa M,Kato A,Hojo H,Shibata Y,Kumamoto N,Watanabe M,Ugawa S

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Genetic mapping of ASIC4 and contrasting phenotype to ASIC1a in modulating innate fear and anxiety.
The European journal of neuroscience41(12)1553-68(2015 Jun)
Lin SH,Chien YC,Chiang WW,Liu YZ,Lien CC,Chen CC

Developmental waves of mechanosensitivity acquisition in sensory neuron subtypes during embryonic development.
The EMBO journal28(10)1479-91(2009 May)
Lechner SG,Frenzel H,Wang R,Lewin GR