pgk1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pgk1
Entrez Gene ID	406696
Full Name	phosphoglycerate kinase 1
Synonyms	wu:fd59b07,wu:fj36g06,zgc:56252,zgc:77899
General protein information	Preferred Names phosphoglycerate kinase 1 Names phosphoglycerate kinase 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_213387.1	NP_998552.1	phosphoglycerate kinase 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PGK1	XP_849992.1
Danio rerio (zebrafish)	pgk1	NP_998552.1
Schizosaccharomyces pombe (fission yeast)	pgk1	NP_596730.1
	PGK1	XP_001100787.1
Drosophila melanogaster (fruit fly)	Pgk	NP_001259958.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008802	XP_314928.2
Arabidopsis thaliana (thale cress)	PGK1	NP_187884.1
Mus musculus (house mouse)	Pgk1	NP_032854.2
Rattus norvegicus (Norway rat)	Pgk1	NP_445743.2
Gallus gallus (chicken)	PGK1	NP_990316.1
Saccharomyces cerevisiae (baker's yeast)	PGK1	NP_009938.2
Arabidopsis thaliana (thale cress)	AT1G56190	NP_176015.1
Pan troglodytes (chimpanzee)	PGK1	NP_001129210.1
Caenorhabditis elegans (roundworm)	pgk-1	NP_491245.1
Kluyveromyces lactis	KLLA0A11011g	XP_451479.1
Eremothecium gossypii	AGOS_AEL038C	NP_984823.2
Magnaporthe oryzae (rice blast fungus)	MGG_05063	XP_003712576.1
Neurospora crassa	NCU07914	XP_962430.1
Homo sapiens (human)	PGK1	NP_000282.1
Bos taurus (cattle)	PGK1	NP_001029471.1
Xenopus tropicalis (tropical clawed frog)	pgk1	NP_001016545.1

Gene Ontology
Bibliography

Related articles in PubMed

Bioenergetic status modulates motor neuron vulnerability and pathogenesis in a zebrafish model of spinal muscular atrophy.
Boyd PJ, Tu WY, Shorrock HK, Groen EJN, Carter RN, Powis RA, Thomson SR, Thomson D, Graham LC, Motyl AAL, Wishart TM, Highley JR, Morton NM, Becker T, Becker CG, Heath PR, Gillingwater TH
PLoS genetics13(4)e1006744(2017 Apr)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Function-driven discovery of disease genes in zebrafish using an integrated genomics big data resource.
Shim H, Kim JH, Kim CY, Hwang S, Kim H, Yang S, Lee JE, Lee I
Nucleic acids research44(20)9611-9623(2016 Nov)

FOXN3 Regulates Hepatic Glucose Utilization.
Karanth S, Zinkhan EK, Hill JT, Yost HJ, Schlegel A
Cell reports15(12)2745-55(2016 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

targeting of a single bioenergetic protein, phosphoglycerate kinase 1 (Pgk1), was found to modulate motor neuron vulnerability in vivo. Knockdown of pgk1 alone was sufficient to partially mimic the SMA phenotype in wild-type zebrafish
Title: Bioenergetic status modulates motor neuron vulnerability and pathogenesis in a zebrafish model of spinal muscular atrophy.

The following pgk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pgk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38578	NM_213387.1 Latest version!	Danio rerio phosphoglycerate kinase 1 (pgk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pgk1 ( NM_213387.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998552.1 Danio rerio phosphoglycerate kinase 1 (pgk1), mRNA.

Price & Availability↑

CloneID	ODa38578
Clone ID Related Accession (Same CDS sequence)	NM_213387.1
Accession Version	NM_213387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Danio rerio(zebrafish)
Product	phosphoglycerate kinase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC046026.1. On Aug 27, 2004 this sequence version replaced NM_213244.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC046026.1, BC065888.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCTTT CGAACAAACT TCATTTGGAC AAGGTGGACG TGAAAGGAAA GCGGGTGATC 
ATGAGGGTGG ACTTCAATGT CCCTATGAAG AACAATGTGA TCACAAATAA CCAGAGAATC 
AAGGCTGCAG TCCCATCTAT CCAGCACTGT TTAGATAATT GTGCCAAAGC TGTGGCCCTG 
ATGAGCCACT TGGGCCGGCC TGATGGAGTC CCTATGCCAG ACAAATACTC TCTGGAGCCT 
GTGGCTGCAG AACTCAAGAA CCTGCTGGGA AAAGACGTTC AGTTCCTGAA AGACTGTGTG 
GGTCCAGATG TGGAGAAAGC CTGTGCAGAC CCTCCTGCAG GATCTGTCAT TCTGCTGGAG 
AATCTGCGCT TTCATGTGGC TGAGGAGGGC AAGGGCAAAG ATGCATCGGG TAACAAGACC 
AAAGCAAGTC AGGCAGAGAT CGATGCTTTC CGAGCCTCCC TGTCCAAATT GGGTGACGTA 
TACGTCAACG ATGCTTTCGG AACTGCACAT AGAGCTCACA GCTCTATGGT TGGAGTGAAT 
CTCCCTCAGA AGGCTGCTGG TTTTCTGATG AAGAAAGAGC TAGACTACTT TGCCATGGCC 
CTTGAGAAAC CACAGAGGCC TTTCTTGGCC ATCCTCGGAG GGGCCAAGGT CAAAGATAAG 
ATTCAGCTGA TCAACAACAT GTTGGACAAG GTGAATGAGA TGATCATTGG TGGAGGAATG 
GCCTTTACCT TCCTCAAGGT TCTCAAGAAC ATGGAGATCG GCACTTCCCT GTTTGATGAA 
GAGGGTTCCA CAATTGTGAA AGACCTCATG GCCAAAGCAG AAAAGAACGG TGTAAAGATC 
ACACTTCCAG TCGACTTTAT CACTGCAGAC AAATTTGATG AGAAGGCAAC AACAGGGACC 
GCAACGGTAG CAGAAGGTAT TCCTGCTGGC TGGATGGGTC TAGACTGTGG TCCTGAGAGC 
TCAAAGCTCT ATGCAGAGGC TGTGGCCAGA GCCAAGCAGA TTGTGTGGAA CGGACCCGTT 
GGCGTGTTCG AGTGGGACAA CTTTGCTCAT GGAACCAAGA ACATGATGGA CAAAGTTGTA 
GAAGCGACCA AAAATGGCTG CATCACCATT ATCGGTGGGG GAGACACAGC TACCTGCTGT 
GCCAAGTGGG ACACTGAGGA CAAGGTCAGC CATGTGAGCA CAGGGGGCGG AGCCAGTCTG 
GAGCTGCTGG AGGGTAAAGT GCTGCCCGGT GTCGATGCCC TCAGTAACGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998552.1
CDS	66..1319
Translation	MSLSNKLHLDKVDVKGKRVIMRVDFNVPMKNNVITNNQRIKAAVPSIQHCLDNCAKAVALMSHLGRPDGVPMPDKYSLEPVAAELKNLLGKDVQFLKDCVGPDVEKACADPPAGSVILLENLRFHVAEEGKGKDASGNKTKASQAEIDAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAAGFLMKKELDYFAMALEKPQRPFLAILGGAKVKDKIQLINNMLDKVNEMIIGGGMAFTFLKVLKNMEIGTSLFDEEGSTIVKDLMAKAEKNGVKITLPVDFITADKFDEKATTGTATVAEGIPAGWMGLDCGPESSKLYAEAVARAKQIVWNGPVGVFEWDNFAHGTKNMMDKVVEATKNGCITIIGGGDTATCCAKWDTEDKVSHVSTGGGASLELLEGKVLPGVDALSNV

Target ORF information:

RefSeq Version	NM_213387.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphoglycerate kinase 1 (pgk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213387.1

ORF Insert Sequence:

ATGTCTCTTT CGAACAAACT TCATTTGGAC AAGGTGGACG TGAAAGGAAA GCGGGTGATC 
ATGAGGGTGG ACTTCAATGT CCCTATGAAG AACAATGTGA TCACAAATAA CCAGAGAATC 
AAGGCTGCAG TCCCATCTAT CCAGCACTGT TTAGATAATT GTGCCAAAGC TGTGGCCCTG 
ATGAGCCACT TGGGCCGGCC TGATGGAGTC CCTATGCCAG ACAAATACTC TCTGGAGCCT 
GTGGCTGCAG AACTCAAGAA CCTGCTGGGA AAAGACGTTC AGTTCCTGAA AGACTGTGTG 
GGTCCAGATG TGGAGAAAGC CTGTGCAGAC CCTCCTGCAG GATCTGTCAT TCTGCTGGAG 
AATCTGCGCT TTCATGTGGC TGAGGAGGGC AAGGGCAAAG ATGCATCGGG TAACAAGACC 
AAAGCAAGTC AGGCAGAGAT CGATGCTTTC CGAGCCTCCC TGTCCAAATT GGGTGACGTA 
TACGTCAACG ATGCTTTCGG AACTGCACAT AGAGCTCACA GCTCTATGGT TGGAGTGAAT 
CTCCCTCAGA AGGCTGCTGG TTTTCTGATG AAGAAAGAGC TAGACTACTT TGCCATGGCC 
CTTGAGAAAC CACAGAGGCC TTTCTTGGCC ATCCTCGGAG GGGCCAAGGT CAAAGATAAG 
ATTCAGCTGA TCAACAACAT GTTGGACAAG GTGAATGAGA TGATCATTGG TGGAGGAATG 
GCCTTTACCT TCCTCAAGGT TCTCAAGAAC ATGGAGATCG GCACTTCCCT GTTTGATGAA 
GAGGGTTCCA CAATTGTGAA AGACCTCATG GCCAAAGCAG AAAAGAACGG TGTAAAGATC 
ACACTTCCAG TCGACTTTAT CACTGCAGAC AAATTTGATG AGAAGGCAAC AACAGGGACC 
GCAACGGTAG CAGAAGGTAT TCCTGCTGGC TGGATGGGTC TAGACTGTGG TCCTGAGAGC 
TCAAAGCTCT ATGCAGAGGC TGTGGCCAGA GCCAAGCAGA TTGTGTGGAA CGGACCCGTT 
GGCGTGTTCG AGTGGGACAA CTTTGCTCAT GGAACCAAGA ACATGATGGA CAAAGTTGTA 
GAAGCGACCA AAAATGGCTG CATCACCATT ATCGGTGGGG GAGACACAGC TACCTGCTGT 
GCCAAGTGGG ACACTGAGGA CAAGGTCAGC CATGTGAGCA CAGGGGGCGG AGCCAGTCTG 
GAGCTGCTGG AGGGTAAAGT GCTGCCCGGT GTCGATGCCC TCAGTAACGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Bioenergetic status modulates motor neuron vulnerability and pathogenesis in a zebrafish model of spinal muscular atrophy.
PLoS genetics13(4)e1006744(2017 Apr)
Boyd PJ,Tu WY,Shorrock HK,Groen EJN,Carter RN,Powis RA,Thomson SR,Thomson D,Graham LC,Motyl AAL,Wishart TM,Highley JR,Morton NM,Becker T,Becker CG,Heath PR,Gillingwater TH

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Function-driven discovery of disease genes in zebrafish using an integrated genomics big data resource.
Nucleic acids research44(20)9611-9623(2016 Nov)
Shim H,Kim JH,Kim CY,Hwang S,Kim H,Yang S,Lee JE,Lee I

FOXN3 Regulates Hepatic Glucose Utilization.
Cell reports15(12)2745-55(2016 06)
Karanth S,Zinkhan EK,Hill JT,Yost HJ,Schlegel A