lgmn cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lgmn
Entrez Gene ID	406625
Full Name	legumain
Synonyms	cb173,cb460,sb:cb173,wu:fa98g05,wu:fj92g08,zgc:76953
General protein information	Preferred Names legumain Names legumain AEP
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_214759.1	NP_999924.1	legumain precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LGMN	NP_776526.1
Rattus norvegicus (Norway rat)	Lgmn	NP_071562.2
Homo sapiens (human)	LGMN	NP_001008530.1
Xenopus tropicalis (tropical clawed frog)	lgmn	NP_001005720.1
	LGMN	XP_001092047.1
Mus musculus (house mouse)	Lgmn	NP_035305.1
Pan troglodytes (chimpanzee)	LGMN	XP_001148301.1
Canis lupus familiaris (dog)	LGMN	XP_537355.2
Gallus gallus (chicken)	LGMN	XP_421328.1
Danio rerio (zebrafish)	lgmn	NP_999924.1
Caenorhabditis elegans (roundworm)	T28H10.3	NP_506137.1
Arabidopsis thaliana (thale cress)	DELTA-VPE	NP_188656.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptome Analysis Reveals Increases in Visceral Lipogenesis and Storage and Activation of the Antigen Processing and Presentation Pathway during the Mouth-Opening Stage in Zebrafish Larvae.
Xu H, Liu E, Li Y, Li X, Ding C
International journal of molecular sciences18(8)(2017 Jul)

Legumain Regulates Differentiation Fate of Human Bone Marrow Stromal Cells and Is Altered in Postmenopausal Osteoporosis.
Jafari A, Qanie D, Andersen TL, Zhang Y, Chen L, Postert B, Parsons S, Ditzel N, Khosla S, Johansen HT, Kj?rsgaard-Andersen P, Delaisse JM, Abdallah BM, Hesselson D, Solberg R, Kassem M
Stem cell reports8(2)373-386(2017 02)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The asparaginyl endopeptidase legumain is essential for functional recovery after spinal cord injury in adult zebrafish.
Ma L, Shen YQ, Khatri HP, Schachner M
PloS one9(4)e95098(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following lgmn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lgmn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36144	NM_214759.1 Latest version!	Danio rerio legumain (lgmn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lgmn ( NM_214759.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_999924.1 Danio rerio legumain (lgmn), mRNA.

Price & Availability↑

CloneID	ODa36144
Clone ID Related Accession (Same CDS sequence)	NM_214759.1
Accession Version	NM_214759.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	legumain precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC066568.1. ##Evidence-Data-START## Transcript exon combination :: BC066568.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAGCCCAA AGACAGTAGC AGTTTTAGGT CTCGCCCTGA GCCTTGGGCT AGTTGTAAGT 
GGTTTTCCTG CGGAACAGCC AGAAAATGGG AAGCACTGGG TCGTCATTGT GGCTGGGTCA 
AATGGCTGGT ACAATTACAG ACACCAGGCT GATGTGTGCC ATGCGTATCA GATTGTCCAT 
AAGAATGGGA TTCCTGATGA GCAGATTGTT GTCATGATGT ATGATGATCT TGCTGAGAGT 
CCTGATAACC CTACAAAAGG AGTGGTCATA AACAGACCAA ATGGATCAGA TGTTTACAAA 
GGAGTGCTAA AGGACTACAT TGGGGACGAC GTCACTCCTG AGAACTTCCT GGCGGTGTTG 
AAAGGTGATG CTGCTAGTGT GAAAGGAGGA TCTGGAAAAG TGCTGAAAAG CGGTCCGAAT 
GATCATGTGT TTGTGTATTT CACTGATCAC GGAGCACCAG GTCTGCTGGC CTTCCCTAAT 
GATGATCTCC ATGTTGACGA CCTGATGGAT ACCATCAAGT ACATGCACAG CAACAACAAA 
TACAAGAAGA TGGTGTTTTA CGTTGAGGCC TGTGAATCTG GCTCCATGAT GAAACCTCTG 
CCTGTTGACA TTAATGTTTA CGCTACCACC GCTGCTAACC CTGATGAGTC CTCATACGCC 
TGCTACTACG ACGAAGCCAG AGACACTTAT CTGGGAGACT GGTACAGCGT CAACTGGATG 
GAGGACTCTG ATGTAGAGGA TCTGAGCAAA GAGACTTTGG CCAAACAGTT CAAGATCGTA 
AAGGCCAAAA CCAACACCAG CCATGTGATG CAGTATGGAA ACAAGACGCT GTCCCACATG 
AAGGTGATGG CGTTCCAGGG TAGCTCTAAG GGTCTTGATA AGGCAGTGGA GCCAGTGTCG 
TTGCCTGTGA TCGCTGAACA TGACCTCATG AGCAGCCCCG ATGTGCCACT TGCCATCCTG 
AAGAGAAAAC TGCAGAAGAC CAATGATGTT GATGCTGTTG TTGGTTACCT GAATGAAATC 
CACGCTCATC TACAGGTGCG GGAGCTGCTT GGAAACACTA TGCGTAAGAT CGTGGAGCAT 
GTTGTGCAGG ACAAAGAGGA AGTGCAGGAT TATCTGGATG GACGTTCAGA CCTCACACAG 
TACAATTGTT ACAAAACAGC CGTCAGACAC TACAAGAAAC ACTGCTTCAA CTGGCACGAG 
CAGAAGTTTG AATATGCTCT GAGACACCTG TATGCTCTAG TAAACCTCTG TGAGGGAGGA 
TACCAGGCCC ACAGAATCAC TGCAGCCATG GACGATGTGT GTTACTTCAG GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999924.1
CDS	25..1341
Translation	MSPKTVAVLGLALSLGLVVSGFPAEQPENGKHWVVIVAGSNGWYNYRHQADVCHAYQIVHKNGIPDEQIVVMMYDDLAESPDNPTKGVVINRPNGSDVYKGVLKDYIGDDVTPENFLAVLKGDAASVKGGSGKVLKSGPNDHVFVYFTDHGAPGLLAFPNDDLHVDDLMDTIKYMHSNNKYKKMVFYVEACESGSMMKPLPVDINVYATTAANPDESSYACYYDEARDTYLGDWYSVNWMEDSDVEDLSKETLAKQFKIVKAKTNTSHVMQYGNKTLSHMKVMAFQGSSKGLDKAVEPVSLPVIAEHDLMSSPDVPLAILKRKLQKTNDVDAVVGYLNEIHAHLQVRELLGNTMRKIVEHVVQDKEEVQDYLDGRSDLTQYNCYKTAVRHYKKHCFNWHEQKFEYALRHLYALVNLCEGGYQAHRITAAMDDVCYFRD

Target ORF information:

RefSeq Version	NM_214759.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio legumain (lgmn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214759.1

ORF Insert Sequence:

ATGAGCCCAA AGACAGTAGC AGTTTTAGGT CTCGCCCTGA GCCTTGGGCT AGTTGTAAGT 
GGTTTTCCTG CGGAACAGCC AGAAAATGGG AAGCACTGGG TCGTCATTGT GGCTGGGTCA 
AATGGCTGGT ACAATTACAG ACACCAGGCT GATGTGTGCC ATGCGTATCA GATTGTCCAT 
AAGAATGGGA TTCCTGATGA GCAGATTGTT GTCATGATGT ATGATGATCT TGCTGAGAGT 
CCTGATAACC CTACAAAAGG AGTGGTCATA AACAGACCAA ATGGATCAGA TGTTTACAAA 
GGAGTGCTAA AGGACTACAT TGGGGACGAC GTCACTCCTG AGAACTTCCT GGCGGTGTTG 
AAAGGTGATG CTGCTAGTGT GAAAGGAGGA TCTGGAAAAG TGCTGAAAAG CGGTCCGAAT 
GATCATGTGT TTGTGTATTT CACTGATCAC GGAGCACCAG GTCTGCTGGC CTTCCCTAAT 
GATGATCTCC ATGTTGACGA CCTGATGGAT ACCATCAAGT ACATGCACAG CAACAACAAA 
TACAAGAAGA TGGTGTTTTA CGTTGAGGCC TGTGAATCTG GCTCCATGAT GAAACCTCTG 
CCTGTTGACA TTAATGTTTA CGCTACCACC GCTGCTAACC CTGATGAGTC CTCATACGCC 
TGCTACTACG ACGAAGCCAG AGACACTTAT CTGGGAGACT GGTACAGCGT CAACTGGATG 
GAGGACTCTG ATGTAGAGGA TCTGAGCAAA GAGACTTTGG CCAAACAGTT CAAGATCGTA 
AAGGCCAAAA CCAACACCAG CCATGTGATG CAGTATGGAA ACAAGACGCT GTCCCACATG 
AAGGTGATGG CGTTCCAGGG TAGCTCTAAG GGTCTTGATA AGGCAGTGGA GCCAGTGTCG 
TTGCCTGTGA TCGCTGAACA TGACCTCATG AGCAGCCCCG ATGTGCCACT TGCCATCCTG 
AAGAGAAAAC TGCAGAAGAC CAATGATGTT GATGCTGTTG TTGGTTACCT GAATGAAATC 
CACGCTCATC TACAGGTGCG GGAGCTGCTT GGAAACACTA TGCGTAAGAT CGTGGAGCAT 
GTTGTGCAGG ACAAAGAGGA AGTGCAGGAT TATCTGGATG GACGTTCAGA CCTCACACAG 
TACAATTGTT ACAAAACAGC CGTCAGACAC TACAAGAAAC ACTGCTTCAA CTGGCACGAG 
CAGAAGTTTG AATATGCTCT GAGACACCTG TATGCTCTAG TAAACCTCTG TGAGGGAGGA 
TACCAGGCCC ACAGAATCAC TGCAGCCATG GACGATGTGT GTTACTTCAG GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptome Analysis Reveals Increases in Visceral Lipogenesis and Storage and Activation of the Antigen Processing and Presentation Pathway during the Mouth-Opening Stage in Zebrafish Larvae.
International journal of molecular sciences18(8)(2017 Jul)
Xu H,Liu E,Li Y,Li X,Ding C

Legumain Regulates Differentiation Fate of Human Bone Marrow Stromal Cells and Is Altered in Postmenopausal Osteoporosis.
Stem cell reports8(2)373-386(2017 02)
Jafari A,Qanie D,Andersen TL,Zhang Y,Chen L,Postert B,Parsons S,Ditzel N,Khosla S,Johansen HT,Kj?rsgaard-Andersen P,Delaisse JM,Abdallah BM,Hesselson D,Solberg R,Kassem M

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The asparaginyl endopeptidase legumain is essential for functional recovery after spinal cord injury in adult zebrafish.
PloS one9(4)e95098(2014)
Ma L,Shen YQ,Khatri HP,Schachner M