myf6 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	myf6
Entrez Gene ID	404208
Full Name	myogenic factor 6
Synonyms	herculin,mrf4
General protein information	Preferred Names myogenic factor 6 Names myogenic factor 6 muscle-specific regulatory factor 4 myf-6
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003982.1	NP_001003982.1	myogenic factor 6

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MYF6	XP_001164135.1
Canis lupus familiaris (dog)	MYF6	XP_003432065.1
Rattus norvegicus (Norway rat)	Myf6	NP_037304.1
Xenopus tropicalis (tropical clawed frog)	myf6	NP_001017160.1
Homo sapiens (human)	MYF6	NP_002460.1
	MYF6	XP_001086944.1
Gallus gallus (chicken)	MYF6	NP_001025917.1
Bos taurus (cattle)	MYF6	NP_861527.1
Danio rerio (zebrafish)	myf6	NP_001003982.1
Mus musculus (house mouse)	Myf6	NP_032683.1

Gene Ontology
Bibliography

Related articles in PubMed

Tributyltin induces premature hatching and reduces locomotor activity in zebrafish (Danio rerio) embryos/larvae at environmentally relevant levels.
Liang X, Souders CL 2nd, Zhang J, Martyniuk CJ
Chemosphere189498-506(2017 Dec)

Quercetin, a natural product supplement, impairs mitochondrial bioenergetics and locomotor behavior in larval zebrafish (Danio rerio).
Zhang JL, Laurence Souders C 2nd, Denslow ND, Martyniuk CJ
Toxicology and applied pharmacology32730-38(2017 07)

Depletion of Myostatin b Promotes Somatic Growth and Lipid Metabolism in Zebrafish.
Gao Y, Dai Z, Shi C, Zhai G, Jin X, He J, Lou Q, Yin Z
Frontiers in endocrinology788(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mrf4 but not myog can fully rescue myogenesis in the myod/myf5 double morphant via a selective and robust activation of myod. Rescue does not happen spontaneously, because the gene is expressed only at the onset of muscle differentiation
Title: Induced early expression of mrf4 but not myog rescues myogenesis in the myod/myf5 double-morphant zebrafish embryo.

Data suggest that the transcript Mrf4_tv2 is involved in alignment of muscle fibers, and Mrf4_tv1 might have cooperative function with Mrf4_tv2 in muscle fiber alignment, without affecting the muscle-nerve connection.
Title: Inactivation of zebrafish mrf4 leads to myofibril misalignment and motor axon growth disorganization.

Mrf4 (myf6) is dynamically expressed in differentiated zebrafish skeletal muscle
Title: Mrf4 (myf6) is dynamically expressed in differentiated zebrafish skeletal muscle.

The following myf6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the myf6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38337	NM_001003982.1 Latest version!	Danio rerio myogenic factor 6 (myf6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

myf6 ( NM_001003982.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003982.1 Danio rerio myogenic factor 6 (myf6), mRNA.

Price & Availability↑

CloneID	ODa38337
Clone ID Related Accession (Same CDS sequence)	NM_001003982.1
Accession Version	NM_001003982.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Danio rerio(zebrafish)
Product	myogenic factor 6
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY335193.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY335193.1, BC163748.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168448, SAMEA3505370 [ECO:0000348] ##Evidence-Data-END##

ATGATGGACC TGTTTGAGAC CAACGCTTAT TTTTTCAATG ATTTGCGTTA TCTTGAGGGG 
GACCATGGGA CCTTGGATAT GCCAGGAGTG TCCCCATTGT ACGAGGGGAA TGACAGTCCT 
CTGTCCCCAG GTCAGGATCC TGTCCCGTCG GAAACGGGGT GCGAGAGCAG CGGAGAGGAG 
CACGTCCTCG CTCCACCGGG GCTCCAGGCG CACTGCGAGG GTCAGTGCCT CATGTGGGCT 
TGCAAGATCT GTAAACGGAA ATCTGCTCCT ACTGACCGGC GAAAGGCCGC CACTTTGAGA 
GAACGAAGGC GGCTCAAAAA GATCAACGAA GCTTTTGACG CGCTAAAGAA AAAGACGGTG 
CCTAATCCGA ACCAGAGGCT GCCCAAAGTG GAGATTTTAC GCAGTGCGAT AAACTACATT 
GAGAAACTGC AGGACCTCTT GCATTCGCTG GATGAGCAGG AGCAGAGCAA TGACACTGAC 
CCTTACACAT ACAACCTGAA GGAAAACCAT GTGACTCCCA GTGAGTATCA TTGGAAAAAG 
ACCTGCCAGA GCTGGCAGGA GAACCCAGAT CATTCCAGCT CCCAGATGGC AGGTCATAGA 
GAAGGAGCCG TGTTGGAGTC TTCAGAGTCC AGCAGTCTTC GTCGCCTCTC CTCCATTGTG 
GACAGCATTT CCACTGAAGA GCCCAAAGCC CGCTGTCCCA GCCAGATCTC AGAGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003982.1
CDS	1..720
Translation	MMDLFETNAYFFNDLRYLEGDHGTLDMPGVSPLYEGNDSPLSPGQDPVPSETGCESSGEEHVLAPPGLQAHCEGQCLMWACKICKRKSAPTDRRKAATLRERRRLKKINEAFDALKKKTVPNPNQRLPKVEILRSAINYIEKLQDLLHSLDEQEQSNDTDPYTYNLKENHVTPSEYHWKKTCQSWQENPDHSSSQMAGHREGAVLESSESSSLRRLSSIVDSISTEEPKARCPSQISEK

Target ORF information:

RefSeq Version	NM_001003982.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myogenic factor 6 (myf6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003982.1

ORF Insert Sequence:

ATGATGGACC TGTTTGAGAC CAACGCTTAT TTTTTCAATG ATTTGCGTTA TCTTGAGGGG 
GACCATGGGA CCTTGGATAT GCCAGGAGTG TCCCCATTGT ACGAGGGGAA TGACAGTCCT 
CTGTCCCCAG GTCAGGATCC TGTCCCGTCG GAAACGGGGT GCGAGAGCAG CGGAGAGGAG 
CACGTCCTCG CTCCACCGGG GCTCCAGGCG CACTGCGAGG GTCAGTGCCT CATGTGGGCT 
TGCAAGATCT GTAAACGGAA ATCTGCTCCT ACTGACCGGC GAAAGGCCGC CACTTTGAGA 
GAACGAAGGC GGCTCAAAAA GATCAACGAA GCTTTTGACG CGCTAAAGAA AAAGACGGTG 
CCTAATCCGA ACCAGAGGCT GCCCAAAGTG GAGATTTTAC GCAGTGCGAT AAACTACATT 
GAGAAACTGC AGGACCTCTT GCATTCGCTG GATGAGCAGG AGCAGAGCAA TGACACTGAC 
CCTTACACAT ACAACCTGAA GGAAAACCAT GTGACTCCCA GTGAGTATCA TTGGAAAAAG 
ACCTGCCAGA GCTGGCAGGA GAACCCAGAT CATTCCAGCT CCCAGATGGC AGGTCATAGA 
GAAGGAGCCG TGTTGGAGTC TTCAGAGTCC AGCAGTCTTC GTCGCCTCTC CTCCATTGTG 
GACAGCATTT CCACTGAAGA GCCCAAAGCC CGCTGTCCCA GCCAGATCTC AGAGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tributyltin induces premature hatching and reduces locomotor activity in zebrafish (Danio rerio) embryos/larvae at environmentally relevant levels.
Chemosphere189498-506(2017 Dec)
Liang X,Souders CL 2nd,Zhang J,Martyniuk CJ

Quercetin, a natural product supplement, impairs mitochondrial bioenergetics and locomotor behavior in larval zebrafish (Danio rerio).
Toxicology and applied pharmacology32730-38(2017 07)
Zhang JL,Laurence Souders C 2nd,Denslow ND,Martyniuk CJ

Depletion of Myostatin b Promotes Somatic Growth and Lipid Metabolism in Zebrafish.
Frontiers in endocrinology788(2016)
Gao Y,Dai Z,Shi C,Zhai G,Jin X,He J,Lou Q,Yin Z