MICAL3A gene cDNA ORF clone, Danio rerio(Zebrafish)

mRNA	Protein	Name
XM_017352026.1	XP_017207515.1	protein-methionine sulfoxide oxidase mical3a isoform X5
XM_021473237.1	XP_021328912.1	protein-methionine sulfoxide oxidase mical3a isoform X4
XM_009293465.2	XP_009291740.1	protein-methionine sulfoxide oxidase mical3a isoform X1
XM_021473239.1	XP_021328914.1	protein-methionine sulfoxide oxidase mical3a isoform X6
XM_009293470.2	XP_009291745.1	protein-methionine sulfoxide oxidase mical3a isoform X2
XM_021473238.1	XP_021328913.1	protein-methionine sulfoxide oxidase mical3a isoform X5
XM_021467760.1	XP_021323435.1	protein-methionine sulfoxide oxidase mical3a isoform X1
XM_021467761.1	XP_021323436.1	protein-methionine sulfoxide oxidase mical3a isoform X2
XM_021467762.1	XP_021323437.1	protein-methionine sulfoxide oxidase mical3a isoform X3
XM_009293471.2	XP_009291746.1	protein-methionine sulfoxide oxidase mical3a isoform X3
XM_009293472.2	XP_009291747.1	protein-methionine sulfoxide oxidase mical3a isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa55882	XM_017352026.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa40698	XM_009293465.2 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1161.30 $1659.00
ODa40701	XM_009293470.2 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1161.30 $1659.00
ODa40702	XM_009293471.2 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1161.30 $1659.00
ODa40703	XM_009293472.2 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1161.30 $1659.00
ODa145981	XM_021467760.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa145981	XM_021473237.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa145982	XM_021473238.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa145982	XM_021467761.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa145983	XM_021467762.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa145983	XM_021473239.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

MICAL3A ( XM_017352026.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017207515.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa55882
Clone ID Related Accession (Same CDS sequence)	XM_017352026.1
Accession Version	XM_017352026.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein-methionine sulfoxide oxidase mical3a isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAAATT 
CTGCTTGAGA AGCAATTGCA AGGTAATTTT TGCAGGCGAC TCCGAATGCA TGACGTTCAG 
AAAGCTTTGC GCAGTTTACT TTATGAAACG AATAACTCGG ATATGCAATG GAAAGATTTA 
AGCGCTTTAG ACTTAAATGC AACGATGCAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207515.1
CDS	631..2343
Translation	MGDGGVNAVGEGVNQSHMLFDRFVQATTCKGTLKAFQELCDFLELKPNEYRVFYHKLKSKLNYWKAKALWAKLDKRASHKEYKKGRACANTKCLIIGAGPCGLRTAIELGFLGAKVVLLEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGIEIHVNVEFKGLIEPPEDQENERIGWRAEVHPRTHPVNELEFDVIIGADGRRNTLSGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILHDYADTEMLLSRANVDQKALLSYAREAADFSTNHQLPKLDFAINHYGQPDVAMFDFTCMYASENAALVRQRNGHKLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWAHGSSPLEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNISLHQVRPNQVRHLLDTGETRDLRVDLENVVNSSTPKLTRNEILLEKQLQGNFCRRLRMHDVQKALRSLLYETNNSDMQWKDLSALDLNATMH

Target ORF information:

RefSeq Version	XM_017352026.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352026.1

ORF Insert Sequence:

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAAATT 
CTGCTTGAGA AGCAATTGCA AGGTAATTTT TGCAGGCGAC TCCGAATGCA TGACGTTCAG 
AAAGCTTTGC GCAGTTTACT TTATGAAACG AATAACTCGG ATATGCAATG GAAAGATTTA 
AGCGCTTTAG ACTTAAATGC AACGATGCAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3A ( XM_009293465.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009291740.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40698
Clone ID Related Accession (Same CDS sequence)	XM_009293465.2
Accession Version	XM_009293465.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein-methionine sulfoxide oxidase mical3a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293465.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAGTCC 
ATCGTGCGCT CCAGTAAGCT GCTGAACTGG TGTCAGAGGC AGACGGAAGG CTACCGAGGT 
GTCAGCGTGT CTGATCTCAC CACGTCCTGG AAGAGTGGTT TGGCCCTGTG CGCGCTCATC 
CACAGATACA GACCAGACCT CATTGACTTT GAATCACTGG ACGAGAAAGA CGTGGAGAAG 
AACAACCAGC TGGCTTTCGA CGTGGCTGAA AGAGAGTTTG GGATTTCTCC CATCATGACC 
GGGAAGGAGA TGTCTGTTGT GGTGGAGCCA GATAAGCTCT CCATGGTGAT GTACCTCAGT 
CAGTTCTACG AGATGTTCAA GGACACGGTG CCACCTGGTG AAAACCAGAA TCTGAGCCCT 
GAGGAGAAGG CCGCACTGAT CGCCAGCACT AAATCACCCA TCTCATTCCT TAGTAAGCTA 
GGTCAAAGCA TCGCAATCTC CCGCAAAAGA AACCCCAAGG ATAAAAAAGA AAAGGAGCTG 
GATGGACTAG GGAAAAGACG AAAGACGAGC CAGGCTGGAC AATCGGAGGA CGAGGAGCTG 
CAGAGGGCTA ATCGAGACGA CAGGCCATCT ATAGCCACAG CTCTGGCAGA ACGTAAGATC 
GACTCCGCTG CAGCAGCCAA CAATAACAAC AAAGTGAAGT CGATGGCCAC GCAGCTGCTG 
GCCAAGTTTG AGGAGAACGC GCCGACACAG TCCACTGGGC TCAAACGACA GGGAGACTCT 
ATGCCCGATC TGGGCCTCAT ACTGACCCCT TCTCCAGCCG CTGCTCCTCT GAACGAGTCA 
GTTCGTCTGG CTCCAGTGCC TGCATGGAGG AAGAGGCGCA CACAGTTACA GGAGCAGATG 
AGTTTCCGCT ATAAAGAAAA ACTCAAGTGT CAAACTCTCC CCATCACACA GGAGCAGGCC 
AGCAGTGGGA CTGAGGTCTT GAGGAGCTGC CCTAAGAAAA CCATTCTTCT TTCTTCTTCC 
TCACCCTCAT CTTCCTCATT CTCTTCATCG TTTCCTCCAC ATGCACAGGG TTCCTTTCGA 
AAGGAGTTCC CTCAGAACAT CGGTGGCAGC GATGTGTGTT TCTTCTGCCG TAAACGGGTC 
TATGTGATGG AGCGTCTCAG CGCTGAAGGA AAGTTTTTCC ATCGCAGCTG CTTCAAGTGC 
GACTACTGCG GCACAACCCT CCGCCTGTCC TCATATGCCT TCGATGTTGA AGACGGGAAG 
TTTTATTGTA AACCACACTA CTGCTACCGA CTCTCTGGTG TGGCACAGAG AAAACGGCCT 
GCACCTGCTG CTGCTCCAGC AAACGCCAAG GAGCCCCAGG TGTTGGCGGT ATCCCCCAAC 
ACAGTGGACG CCCCAGGTCA AGCCATAACC ACTCAGACCC CTGCGGAGCG CCGGCCCTCA 
GAAACTGAGG TAAATGGTGT CACGGAGCCC AGCGTGGCCA AACGTCTGAA AGGGACTCCG 
GAAAGGATCG AGCTGGAGAA CTATCGTTTG TCAATGATGA GGGAGGAGGA GCTGGAGGAG 
GTGCCGGAGG AGACATTGGC GGAGCACAAC CTCAGCAGTG TGCTGGATAA AGCCACCGAC 
ATCGAGGAGG GATCCAGCAG CTCTGAGTCT GACATGGAGG AAGAGGATGA AGATGCAGAG 
GCGGCCGGTC CGTCTGATCT GGGTGGAGTT CCCTGGAAGG AGGCAGTAGA GCTCCATGCT 
AAGTTAAAGG GAGAAAGCGA CCCAGGAGCT GACGATGATG GCCTTCATGA CGGTGATGGA 
GAGATGGACG AGGATGAAGA AGAGGAGGAA GATGAGGAAG ATGAAGATGA GGAGGAAGAA 
GAGGAATCGA GCGAAGCGGG AGACTATTTC CCCTGGGAGA GGGAGCTTCA GTCAGGCCTT 
TGGCTGGAAA ATCTGGCAAA TGAAGAGGAT GTCAGTGTTT TTAAAGCTAG GAACCTGCAG 
ATCCAGCAAG CCATTCATCC AGTAGACCCT CTGGACATCC AGATGGAGAG CCATCAGGCT 
GAGGGGGTGT TGGAGATGCC CTCGGTGTCA CCCTTAGGCT CACAGCCCTC GCTCAACACC 
CAGCTCACAC AGCCCACCTC CTCCACAGCC CCCTCCCACA AATCCGTACG TCACGAGGCT 
GTCAGAGCCT GGCTGGACTC GATGTCTGGA GAGCCCTGTG AAGAGGACGA TGACCCTGAA 
GCTGAAGCAG GCAGTCCTGA CTTTGAGCCA GGCACTGAAA TAGATCAAGA GGACATTCCC 
TCAGATGCAG AGGCTGAAGC TCGTTCACGG TGCATAGATG AAGTGGTGAC ACTTCCAGTG 
GATAATGATA AAACAGAAAG CCAGGGACAG GTTTTCAACA CTGCAGAGAA AACGTCTGCA 
AATCCAAGGG AGTTGATTGT GGACGTTGTG CTGTCTCCAA TCCAAAAACC TGCTTTACCC 
ATTGAGGAGG TGCATGAAGT TTCTCCTGTA GTACTAGTTA AATCTCCTGG TGCACGTTTC 
TTCCCTGAGC CCTATCTGCC TGATAAAGTC AAGCAGAACA TTCCCCCGCC TCAGAGTCCT 
GATGTTAAAA CCCCTCATTC TCCTGTAGCT CAAATCATTG CGCTGTCGCC CATCTGTTCC 
CAGCCTGTTC CACAACCAGG CACAGCATCT CCCAAATCTC CAGTCCAACC ACAGCCTTGT 
GCCTGTTCTC CAACAGGCAA TCCTTTGTCT CCGATTTGTA CCCAATCGCA ACCCTGCAAT 
GAGCCACCTT CACCCCTATC CACAAGCTCC CCCGTCAGGA CTCAACCAGT CCCAGCAGTA 
ACTTCTACTC CTTTAGCTAA ACCTGCTTCC GAGAATAGGA CTAATGAACA TTTGAAAGAT 
TCAACTCCTG AGCTTAAGAA AACAGACCTC ATTGAGGAGT TCTGGTTGAA GAGTGCAGAG 
ATTAGAAAAA GTTTAGGGCT CACTCCCCTG GAACGAAGCA AAACTGCGGT GGAGAAGAGC 
ATTGTGAAAA CTCCAACACC CGAATCATCA TCTCCTAAGT CTTATACCCC AGAGGACTTG 
TCTGAGGAAC AGAAGCCTAC ATTTACGGGC CGTTCTATCA TACGCAGGAT CAATATTACC 
TTGGAGGGTC AGGTTATATC TCCGGTGGAA CCTAAAAGTA ATGGTTCAGA AAAGAAGGAC 
TTAAGCAGCA GTTCAGGGTT GGGTCTAAAC GGAAGTGTGA CCACCAGTCA GACAGCTGCT 
AGCGACAGCT ACAACAACTC TGATTCTACA ATGCTTACCC CTCCTTCCAG TCCACCACCT 
CCTCCACCCA GGGAGGAACC TGCTTGTCTC CAAAACAAGA AGTCTCAGGT TTCCTGGGAC 
AACCTCCTTG AAGGCACAGA AGAACCCAAA TCTGAGACCA TGCCCATTAA GCCCAGGACT 
CCAGTTAGTC CCCCTCAACC AAAACAAAAA CCAGTTACAG CTCCAGTGCC AACCCCTAGA 
ACCAATCCAC CTGTTGTGAT GAGGGTTAAG GAGCCCAATA AACCACGTCG AGAAGAAGTG 
AGAAAGTCTT TTGTTGAGTG TGTAGACGAG ATACCATTTG CTGATGATGT TGAAGACACA 
TACGATGACC GCACTCCAGA TGCAAGTGGT CTTGAAAAGT TTTACACACC GCCGACTAGC 
AAGGTCAACC GGGATAAGCC GCCTCTCCAC TTGGCACTGG CTATGGAAAA TGGCAAGCCC 
AATATCCCTG GAGTTTCCCG AACAGCAAAG GGTTCCCAGC ATTTTTCTCC TGAAGCCAAG 
GAGATCGCAG AGGAGCGAAT GAGGGCACGG GAAAAGTCTG TGAAGAGCCA AGCTCTTAAA 
GATGCAATGG CCAAGCAGCT TACAAAGATG AAAGATTCTG AGGTTGCCAA GGGGGCTGTA 
GCAAAAGTAG CCTGGGATAT TCCTGAAACA AAAGGAAAAA GCAAAAAGCA ATCTAAAGCC 
CAGAAGGACT CTGCAGTGAA GGCCTTGGAG TCCAAAAAGC AAGCAGACAC ACTTCCAGAC 
CGTTTCTTCT CTACACCATC TAGTAAGGCC TTAGACAGCT CTGTCACATC TTCAGAGAGC 
TCCACAGGGG GCAAAAGCAA GAAACGCAGC TCACTGTTTT CTCCACGCAA AAACAAAAAA 
GAAAAGAAAG CCAAAAATGA AAGACTGTCC AGCACTGAAG AAACGCCGCC CAAGCACAAG 
TCCCTTTGGA AAGCAGTGTT CTCCGGCTAC AAGAAGGACA AAAAGAAGAA AGATGACAAG 
TCATGCCCAA GTACACCCTC AAGCTCTACA ACTGGAGACT CGGGGAAGAA GAAAGATTCA 
CCCCTGGACA GGTCCTCAGA TTTACGTCTG AGGAGAAATC TAAGCTTTTC TGAGGACTCT 
GATCTCTCCT GTGACGATGT TCTGGAAAGA TCCTCTCAGA AGTCCAAGGG TGATCGTCAT 
GTGGACCTGT TTGACTTGGT GTCAGATTTG AAAAAAGAAG CCATTAAAGA GGAGAAAGCA 
AAAGAGAAGG CTAAGGATAG GGCAAAGGAA AGGGGAAGAG TTACTGAGAA GGGCAAGGAA 
AGGTCAAGAG AAAGGGAGAA TGAAAAAAGA GTCAAAGAAA AGATGGTGAA AATGAAAGAT 
GTGGAACGGG AGAAAGGAAA AGAGGAAGAG TCTGTTTATG TACCTCATGC ATTGGCGTTC 
AAGAGATCAT ATGCCACAAA GAAGACATAC ACGGAGGAGG AACTGAACGC CAAGCTGACA 
AGAAGAGTAC AGAAAGCTGC ACGGAGGCAG GCCAAGCAGG AGGAGCTCAA GAGACTTCAC 
AGAGCTCAGA TGATTCAGAG GCAGCTGGAG CAGGTGGAAG AGAAACAGAG GCAGTTAGAG 
GAGAGAGGCG TCGCGGTGGA AAAGGCACTG CGAGGCGAAG CAGTTGAGCC CTATGTCGGG 
ACCCCTCGCC GAAGAGCCCT GTACCTCTGC CCCTGCTGTG CCCCTGAAGG AATGGGTAAA 
AAGGACGATC CGAAGCTGAT GCAGGAATGG TTTAAACTGG TCCAGGAGAA GAATGCCTTG 
GTACGATATG AATCCGAGCT GATGATATTT GCTCGGGAGC TAGAGTTGGA GGACAGACAG 
AGTCGACTGC AACAGGAACT ACGGGAACGC ATGGCAATAG ATGATCATCT GAAGACAGAG 
GAGGAGCTGG CAGAGGAGAA GCAGATTCTG AATGAGATGT TGGAGGTGGT GGAGCAGCGA 
GACTCTCTGG TGGCCCTGCT GGAGGAACAG AGACTCAGGG AGAAGGAAGA AGATAAAGAC 
CTGGAGGCGG TGATGCTCTC CAAAGGATTC AACCTCAACT GGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291740.1
CDS	600..7427
Translation	MGDGGVNAVGEGVNQSHMLFDRFVQATTCKGTLKAFQELCDFLELKPNEYRVFYHKLKSKLNYWKAKALWAKLDKRASHKEYKKGRACANTKCLIIGAGPCGLRTAIELGFLGAKVVLLEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGIEIHVNVEFKGLIEPPEDQENERIGWRAEVHPRTHPVNELEFDVIIGADGRRNTLSGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILHDYADTEMLLSRANVDQKALLSYAREAADFSTNHQLPKLDFAINHYGQPDVAMFDFTCMYASENAALVRQRNGHKLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWAHGSSPLEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNISLHQVRPNQVRHLLDTGETRDLRVDLENVVNSSTPKLTRNESIVRSSKLLNWCQRQTEGYRGVSVSDLTTSWKSGLALCALIHRYRPDLIDFESLDEKDVEKNNQLAFDVAEREFGISPIMTGKEMSVVVEPDKLSMVMYLSQFYEMFKDTVPPGENQNLSPEEKAALIASTKSPISFLSKLGQSIAISRKRNPKDKKEKELDGLGKRRKTSQAGQSEDEELQRANRDDRPSIATALAERKIDSAAAANNNNKVKSMATQLLAKFEENAPTQSTGLKRQGDSMPDLGLILTPSPAAAPLNESVRLAPVPAWRKRRTQLQEQMSFRYKEKLKCQTLPITQEQASSGTEVLRSCPKKTILLSSSSPSSSSFSSSFPPHAQGSFRKEFPQNIGGSDVCFFCRKRVYVMERLSAEGKFFHRSCFKCDYCGTTLRLSSYAFDVEDGKFYCKPHYCYRLSGVAQRKRPAPAAAPANAKEPQVLAVSPNTVDAPGQAITTQTPAERRPSETEVNGVTEPSVAKRLKGTPERIELENYRLSMMREEELEEVPEETLAEHNLSSVLDKATDIEEGSSSSESDMEEEDEDAEAAGPSDLGGVPWKEAVELHAKLKGESDPGADDDGLHDGDGEMDEDEEEEEDEEDEDEEEEEESSEAGDYFPWERELQSGLWLENLANEEDVSVFKARNLQIQQAIHPVDPLDIQMESHQAEGVLEMPSVSPLGSQPSLNTQLTQPTSSTAPSHKSVRHEAVRAWLDSMSGEPCEEDDDPEAEAGSPDFEPGTEIDQEDIPSDAEAEARSRCIDEVVTLPVDNDKTESQGQVFNTAEKTSANPRELIVDVVLSPIQKPALPIEEVHEVSPVVLVKSPGARFFPEPYLPDKVKQNIPPPQSPDVKTPHSPVAQIIALSPICSQPVPQPGTASPKSPVQPQPCACSPTGNPLSPICTQSQPCNEPPSPLSTSSPVRTQPVPAVTSTPLAKPASENRTNEHLKDSTPELKKTDLIEEFWLKSAEIRKSLGLTPLERSKTAVEKSIVKTPTPESSSPKSYTPEDLSEEQKPTFTGRSIIRRINITLEGQVISPVEPKSNGSEKKDLSSSSGLGLNGSVTTSQTAASDSYNNSDSTMLTPPSSPPPPPPREEPACLQNKKSQVSWDNLLEGTEEPKSETMPIKPRTPVSPPQPKQKPVTAPVPTPRTNPPVVMRVKEPNKPRREEVRKSFVECVDEIPFADDVEDTYDDRTPDASGLEKFYTPPTSKVNRDKPPLHLALAMENGKPNIPGVSRTAKGSQHFSPEAKEIAEERMRAREKSVKSQALKDAMAKQLTKMKDSEVAKGAVAKVAWDIPETKGKSKKQSKAQKDSAVKALESKKQADTLPDRFFSTPSSKALDSSVTSSESSTGGKSKKRSSLFSPRKNKKEKKAKNERLSSTEETPPKHKSLWKAVFSGYKKDKKKKDDKSCPSTPSSSTTGDSGKKKDSPLDRSSDLRLRRNLSFSEDSDLSCDDVLERSSQKSKGDRHVDLFDLVSDLKKEAIKEEKAKEKAKDRAKERGRVTEKGKERSRERENEKRVKEKMVKMKDVEREKGKEEESVYVPHALAFKRSYATKKTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQMIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPYVGTPRRRALYLCPCCAPEGMGKKDDPKLMQEWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAIDDHLKTEEELAEEKQILNEMLEVVEQRDSLVALLEEQRLREKEEDKDLEAVMLSKGFNLNWA

Target ORF information:

RefSeq Version	XM_009293465.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293465.2

ORF Insert Sequence:

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAGTCC 
ATCGTGCGCT CCAGTAAGCT GCTGAACTGG TGTCAGAGGC AGACGGAAGG CTACCGAGGT 
GTCAGCGTGT CTGATCTCAC CACGTCCTGG AAGAGTGGTT TGGCCCTGTG CGCGCTCATC 
CACAGATACA GACCAGACCT CATTGACTTT GAATCACTGG ACGAGAAAGA CGTGGAGAAG 
AACAACCAGC TGGCTTTCGA CGTGGCTGAA AGAGAGTTTG GGATTTCTCC CATCATGACC 
GGGAAGGAGA TGTCTGTTGT GGTGGAGCCA GATAAGCTCT CCATGGTGAT GTACCTCAGT 
CAGTTCTACG AGATGTTCAA GGACACGGTG CCACCTGGTG AAAACCAGAA TCTGAGCCCT 
GAGGAGAAGG CCGCACTGAT CGCCAGCACT AAATCACCCA TCTCATTCCT TAGTAAGCTA 
GGTCAAAGCA TCGCAATCTC CCGCAAAAGA AACCCCAAGG ATAAAAAAGA AAAGGAGCTG 
GATGGACTAG GGAAAAGACG AAAGACGAGC CAGGCTGGAC AATCGGAGGA CGAGGAGCTG 
CAGAGGGCTA ATCGAGACGA CAGGCCATCT ATAGCCACAG CTCTGGCAGA ACGTAAGATC 
GACTCCGCTG CAGCAGCCAA CAATAACAAC AAAGTGAAGT CGATGGCCAC GCAGCTGCTG 
GCCAAGTTTG AGGAGAACGC GCCGACACAG TCCACTGGGC TCAAACGACA GGGAGACTCT 
ATGCCCGATC TGGGCCTCAT ACTGACCCCT TCTCCAGCCG CTGCTCCTCT GAACGAGTCA 
GTTCGTCTGG CTCCAGTGCC TGCATGGAGG AAGAGGCGCA CACAGTTACA GGAGCAGATG 
AGTTTCCGCT ATAAAGAAAA ACTCAAGTGT CAAACTCTCC CCATCACACA GGAGCAGGCC 
AGCAGTGGGA CTGAGGTCTT GAGGAGCTGC CCTAAGAAAA CCATTCTTCT TTCTTCTTCC 
TCACCCTCAT CTTCCTCATT CTCTTCATCG TTTCCTCCAC ATGCACAGGG TTCCTTTCGA 
AAGGAGTTCC CTCAGAACAT CGGTGGCAGC GATGTGTGTT TCTTCTGCCG TAAACGGGTC 
TATGTGATGG AGCGTCTCAG CGCTGAAGGA AAGTTTTTCC ATCGCAGCTG CTTCAAGTGC 
GACTACTGCG GCACAACCCT CCGCCTGTCC TCATATGCCT TCGATGTTGA AGACGGGAAG 
TTTTATTGTA AACCACACTA CTGCTACCGA CTCTCTGGTG TGGCACAGAG AAAACGGCCT 
GCACCTGCTG CTGCTCCAGC AAACGCCAAG GAGCCCCAGG TGTTGGCGGT ATCCCCCAAC 
ACAGTGGACG CCCCAGGTCA AGCCATAACC ACTCAGACCC CTGCGGAGCG CCGGCCCTCA 
GAAACTGAGG TAAATGGTGT CACGGAGCCC AGCGTGGCCA AACGTCTGAA AGGGACTCCG 
GAAAGGATCG AGCTGGAGAA CTATCGTTTG TCAATGATGA GGGAGGAGGA GCTGGAGGAG 
GTGCCGGAGG AGACATTGGC GGAGCACAAC CTCAGCAGTG TGCTGGATAA AGCCACCGAC 
ATCGAGGAGG GATCCAGCAG CTCTGAGTCT GACATGGAGG AAGAGGATGA AGATGCAGAG 
GCGGCCGGTC CGTCTGATCT GGGTGGAGTT CCCTGGAAGG AGGCAGTAGA GCTCCATGCT 
AAGTTAAAGG GAGAAAGCGA CCCAGGAGCT GACGATGATG GCCTTCATGA CGGTGATGGA 
GAGATGGACG AGGATGAAGA AGAGGAGGAA GATGAGGAAG ATGAAGATGA GGAGGAAGAA 
GAGGAATCGA GCGAAGCGGG AGACTATTTC CCCTGGGAGA GGGAGCTTCA GTCAGGCCTT 
TGGCTGGAAA ATCTGGCAAA TGAAGAGGAT GTCAGTGTTT TTAAAGCTAG GAACCTGCAG 
ATCCAGCAAG CCATTCATCC AGTAGACCCT CTGGACATCC AGATGGAGAG CCATCAGGCT 
GAGGGGGTGT TGGAGATGCC CTCGGTGTCA CCCTTAGGCT CACAGCCCTC GCTCAACACC 
CAGCTCACAC AGCCCACCTC CTCCACAGCC CCCTCCCACA AATCCGTACG TCACGAGGCT 
GTCAGAGCCT GGCTGGACTC GATGTCTGGA GAGCCCTGTG AAGAGGACGA TGACCCTGAA 
GCTGAAGCAG GCAGTCCTGA CTTTGAGCCA GGCACTGAAA TAGATCAAGA GGACATTCCC 
TCAGATGCAG AGGCTGAAGC TCGTTCACGG TGCATAGATG AAGTGGTGAC ACTTCCAGTG 
GATAATGATA AAACAGAAAG CCAGGGACAG GTTTTCAACA CTGCAGAGAA AACGTCTGCA 
AATCCAAGGG AGTTGATTGT GGACGTTGTG CTGTCTCCAA TCCAAAAACC TGCTTTACCC 
ATTGAGGAGG TGCATGAAGT TTCTCCTGTA GTACTAGTTA AATCTCCTGG TGCACGTTTC 
TTCCCTGAGC CCTATCTGCC TGATAAAGTC AAGCAGAACA TTCCCCCGCC TCAGAGTCCT 
GATGTTAAAA CCCCTCATTC TCCTGTAGCT CAAATCATTG CGCTGTCGCC CATCTGTTCC 
CAGCCTGTTC CACAACCAGG CACAGCATCT CCCAAATCTC CAGTCCAACC ACAGCCTTGT 
GCCTGTTCTC CAACAGGCAA TCCTTTGTCT CCGATTTGTA CCCAATCGCA ACCCTGCAAT 
GAGCCACCTT CACCCCTATC CACAAGCTCC CCCGTCAGGA CTCAACCAGT CCCAGCAGTA 
ACTTCTACTC CTTTAGCTAA ACCTGCTTCC GAGAATAGGA CTAATGAACA TTTGAAAGAT 
TCAACTCCTG AGCTTAAGAA AACAGACCTC ATTGAGGAGT TCTGGTTGAA GAGTGCAGAG 
ATTAGAAAAA GTTTAGGGCT CACTCCCCTG GAACGAAGCA AAACTGCGGT GGAGAAGAGC 
ATTGTGAAAA CTCCAACACC CGAATCATCA TCTCCTAAGT CTTATACCCC AGAGGACTTG 
TCTGAGGAAC AGAAGCCTAC ATTTACGGGC CGTTCTATCA TACGCAGGAT CAATATTACC 
TTGGAGGGTC AGGTTATATC TCCGGTGGAA CCTAAAAGTA ATGGTTCAGA AAAGAAGGAC 
TTAAGCAGCA GTTCAGGGTT GGGTCTAAAC GGAAGTGTGA CCACCAGTCA GACAGCTGCT 
AGCGACAGCT ACAACAACTC TGATTCTACA ATGCTTACCC CTCCTTCCAG TCCACCACCT 
CCTCCACCCA GGGAGGAACC TGCTTGTCTC CAAAACAAGA AGTCTCAGGT TTCCTGGGAC 
AACCTCCTTG AAGGCACAGA AGAACCCAAA TCTGAGACCA TGCCCATTAA GCCCAGGACT 
CCAGTTAGTC CCCCTCAACC AAAACAAAAA CCAGTTACAG CTCCAGTGCC AACCCCTAGA 
ACCAATCCAC CTGTTGTGAT GAGGGTTAAG GAGCCCAATA AACCACGTCG AGAAGAAGTG 
AGAAAGTCTT TTGTTGAGTG TGTAGACGAG ATACCATTTG CTGATGATGT TGAAGACACA 
TACGATGACC GCACTCCAGA TGCAAGTGGT CTTGAAAAGT TTTACACACC GCCGACTAGC 
AAGGTCAACC GGGATAAGCC GCCTCTCCAC TTGGCACTGG CTATGGAAAA TGGCAAGCCC 
AATATCCCTG GAGTTTCCCG AACAGCAAAG GGTTCCCAGC ATTTTTCTCC TGAAGCCAAG 
GAGATCGCAG AGGAGCGAAT GAGGGCACGG GAAAAGTCTG TGAAGAGCCA AGCTCTTAAA 
GATGCAATGG CCAAGCAGCT TACAAAGATG AAAGATTCTG AGGTTGCCAA GGGGGCTGTA 
GCAAAAGTAG CCTGGGATAT TCCTGAAACA AAAGGAAAAA GCAAAAAGCA ATCTAAAGCC 
CAGAAGGACT CTGCAGTGAA GGCCTTGGAG TCCAAAAAGC AAGCAGACAC ACTTCCAGAC 
CGTTTCTTCT CTACACCATC TAGTAAGGCC TTAGACAGCT CTGTCACATC TTCAGAGAGC 
TCCACAGGGG GCAAAAGCAA GAAACGCAGC TCACTGTTTT CTCCACGCAA AAACAAAAAA 
GAAAAGAAAG CCAAAAATGA AAGACTGTCC AGCACTGAAG AAACGCCGCC CAAGCACAAG 
TCCCTTTGGA AAGCAGTGTT CTCCGGCTAC AAGAAGGACA AAAAGAAGAA AGATGACAAG 
TCATGCCCAA GTACACCCTC AAGCTCTACA ACTGGAGACT CGGGGAAGAA GAAAGATTCA 
CCCCTGGACA GGTCCTCAGA TTTACGTCTG AGGAGAAATC TAAGCTTTTC TGAGGACTCT 
GATCTCTCCT GTGACGATGT TCTGGAAAGA TCCTCTCAGA AGTCCAAGGG TGATCGTCAT 
GTGGACCTGT TTGACTTGGT GTCAGATTTG AAAAAAGAAG CCATTAAAGA GGAGAAAGCA 
AAAGAGAAGG CTAAGGATAG GGCAAAGGAA AGGGGAAGAG TTACTGAGAA GGGCAAGGAA 
AGGTCAAGAG AAAGGGAGAA TGAAAAAAGA GTCAAAGAAA AGATGGTGAA AATGAAAGAT 
GTGGAACGGG AGAAAGGAAA AGAGGAAGAG TCTGTTTATG TACCTCATGC ATTGGCGTTC 
AAGAGATCAT ATGCCACAAA GAAGACATAC ACGGAGGAGG AACTGAACGC CAAGCTGACA 
AGAAGAGTAC AGAAAGCTGC ACGGAGGCAG GCCAAGCAGG AGGAGCTCAA GAGACTTCAC 
AGAGCTCAGA TGATTCAGAG GCAGCTGGAG CAGGTGGAAG AGAAACAGAG GCAGTTAGAG 
GAGAGAGGCG TCGCGGTGGA AAAGGCACTG CGAGGCGAAG CAGTTGAGCC CTATGTCGGG 
ACCCCTCGCC GAAGAGCCCT GTACCTCTGC CCCTGCTGTG CCCCTGAAGG AATGGGTAAA 
AAGGACGATC CGAAGCTGAT GCAGGAATGG TTTAAACTGG TCCAGGAGAA GAATGCCTTG 
GTACGATATG AATCCGAGCT GATGATATTT GCTCGGGAGC TAGAGTTGGA GGACAGACAG 
AGTCGACTGC AACAGGAACT ACGGGAACGC ATGGCAATAG ATGATCATCT GAAGACAGAG 
GAGGAGCTGG CAGAGGAGAA GCAGATTCTG AATGAGATGT TGGAGGTGGT GGAGCAGCGA 
GACTCTCTGG TGGCCCTGCT GGAGGAACAG AGACTCAGGG AGAAGGAAGA AGATAAAGAC 
CTGGAGGCGG TGATGCTCTC CAAAGGATTC AACCTCAACT GGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3A ( XM_009293470.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009291745.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X2, mRNA.

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CloneID	ODa40701
Clone ID Related Accession (Same CDS sequence)	XM_009293470.2
Accession Version	XM_009293470.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein-methionine sulfoxide oxidase mical3a isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293470.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAGTCC 
ATCGTGCGCT CCAGTAAGCT GCTGAACTGG TGTCAGAGGC AGACGGAAGG CTACCGAGGT 
GTCAGCGTGT CTGATCTCAC CACGTCCTGG AAGAGTGGTT TGGCCCTGTG CGCGCTCATC 
CACAGATACA GACCAGACCT CATTGACTTT GAATCACTGG ACGAGAAAGA CGTGGAGAAG 
AACAACCAGC TGGCTTTCGA CGTGGCTGAA AGAGAGTTTG GGATTTCTCC CATCATGACC 
GGGAAGGAGA TGTCTGTTGT GGTGGAGCCA GATAAGCTCT CCATGGTGAT GTACCTCAGT 
CAGTTCTACG AGATGTTCAA GGACACGGTG CCACCTGGTG AAAACCAGAA TCTGAGCCCT 
GAGGAGAAGG CCGCACTGAT CGCCAGCACT AAATCACCCA TCTCATTCCT TAGTAAGCTA 
GGTCAAAGCA TCGCAATCTC CCGCAAAAGA AACCCCAAGG ATAAAAAAGA AAAGGAGCTG 
GATGGACTAG GGAAAAGACG AAAGACGAGC CAGGCTGGAC AATCGGAGGA CGAGGAGCTG 
CAGAGGGCTA ATCGAGACGA CAGGCCATCT ATAGCCACAG CTCTGGCAGA ACGTAAGATC 
GACTCCGCTG CAGCAGCCAA CAATAACAAC AAAGTGAAGT CGATGGCCAC GCAGCTGCTG 
GCCAAGTTTG AGGAGAACGC GCCGACACAG TCCACTGGGC TCAAACGACA GGGAGACTCT 
ATGCCCGATC TGGGCCTCAT ACTGACCCCT TCTCCAGCCG CTGCTCCTCT GAACGAGTCA 
GTTCGTCTGG CTCCAGTGCC TGCATGGAGG AAGAGGCGCA CACAGTTACA GGAGCAGATG 
AGTTTCCGCT ATAAAGAAAA ACTCAAGTGT CAAACTCTCC CCATCACACA GGAGCAGGCC 
AGCAGTGGGA CTGAGGTCTT GAGGAGCTGC CCTAAGAAAA CCATTCTTCT TTCTTCTTCC 
TCACCCTCAT CTTCCTCATT CTCTTCATCG TTTCCTCCAC ATGCACAGGG TTCCTTTCGA 
AAGGAGTTCC CTCAGAACAT CGGTGGCAGC GATGTGTGTT TCTTCTGCCG TAAACGGGTC 
TATGTGATGG AGCGTCTCAG CGCTGAAGGA AAGTTTTTCC ATCGCAGCTG CTTCAAGTGC 
GACTACTGCG GCACAACCCT CCGCCTGTCC TCATATGCCT TCGATGTTGA AGACGGGAAG 
TTTTATTGTA AACCACACTA CTGCTACCGA CTCTCTGGTG TGGCACAGAG AAAACGGCCT 
GCACCTGCTG CTGCTCCAGC AAACGCCAAG GAGCCCCAGG TGTTGGCGGT ATCCCCCAAC 
ACAGTGGACG CCCCAGGTCA AGCCATAACC ACTCAGACCC CTGCGGAGCG CCGGCCCTCA 
GAAACTGAGG TAAATGGTGT CACGGAGCCC AGCGTGGCCA AACGTCTGAA AGGGACTCCG 
GAAAGGATCG AGCTGGAGAA CTATCGTTTG TCAATGATGA GGGAGGAGGA GCTGGAGGAG 
GTGCCGGAGG AGACATTGGC GGAGCACAAC CTCAGCAGTG TGCTGGATAA AGCCACCGAC 
ATCGAGGAGG GATCCAGCAG CTCTGAGTCT GACATGGAGG AAGAGGATGA AGATGCAGAG 
GCGGCCGGTC CGTCTGATCT GGGTGGAGTT CCCTGGAAGG AGGCAGTAGA GCTCCATGCT 
AAGTTAAAGG GAGAAAGCGA CCCAGGAGCT GACGATGATG GCCTTCATGA CGGTGATGGA 
GAGATGGACG AGGATGAAGA AGAGGAGGAA GATGAGGAAG ATGAAGATGA GGAGGAAGAA 
GAGGAATCGA GCGAAGCGGG AGACTATTTC CCCTGGGAGA GGGAGCTTCA GTCAGGCCTT 
TGGCTGGAAA ATCTGGCAAA TGAAGAGGAT GTCAGTGTTT TTAAAGCTAG GAACCTGCAG 
ATCCAGCAAG CCATTCATCC AGTAGACCCT CTGGACATCC AGATGGAGAG CCATCAGGCT 
GAGGGGGTGT TGGAGATGCC CTCGGTGTCA CCCTTAGGCT CACAGCCCTC GCTCAACACC 
CAGCTCACAC AGCCCACCTC CTCCACAGAG CCCTGTGAAG AGGACGATGA CCCTGAAGCT 
GAAGCAGGCA GTCCTGACTT TGAGCCAGGC ACTGAAATAG ATCAAGAGGA CATTCCCTCA 
GATGCAGAGG CTGAAGCTCG TTCACGGTGC ATAGATGAAG TGGTGACACT TCCAGTGGAT 
AATGATAAAA CAGAAAGCCA GGGACAGGTT TTCAACACTG CAGAGAAAAC GTCTGCAAAT 
CCAAGGGAGT TGATTGTGGA CGTTGTGCTG TCTCCAATCC AAAAACCTGC TTTACCCATT 
GAGGAGGTGC ATGAAGTTTC TCCTGTAGTA CTAGTTAAAT CTCCTGGTGC ACGTTTCTTC 
CCTGAGCCCT ATCTGCCTGA TAAAGTCAAG CAGAACATTC CCCCGCCTCA GAGTCCTGAT 
GTTAAAACCC CTCATTCTCC TGTAGCTCAA ATCATTGCGC TGTCGCCCAT CTGTTCCCAG 
CCTGTTCCAC AACCAGGCAC AGCATCTCCC AAATCTCCAG TCCAACCACA GCCTTGTGCC 
TGTTCTCCAA CAGGCAATCC TTTGTCTCCG ATTTGTACCC AATCGCAACC CTGCAATGAG 
CCACCTTCAC CCCTATCCAC AAGCTCCCCC GTCAGGACTC AACCAGTCCC AGCAGTAACT 
TCTACTCCTT TAGCTAAACC TGCTTCCGAG AATAGGACTA ATGAACATTT GAAAGATTCA 
ACTCCTGAGC TTAAGAAAAC AGACCTCATT GAGGAGTTCT GGTTGAAGAG TGCAGAGATT 
AGAAAAAGTT TAGGGCTCAC TCCCCTGGAA CGAAGCAAAA CTGCGGTGGA GAAGAGCATT 
GTGAAAACTC CAACACCCGA ATCATCATCT CCTAAGTCTT ATACCCCAGA GGACTTGTCT 
GAGGAACAGA AGCCTACATT TACGGGCCGT TCTATCATAC GCAGGATCAA TATTACCTTG 
GAGGGTCAGG TTATATCTCC GGTGGAACCT AAAAGTAATG GTTCAGAAAA GAAGGACTTA 
AGCAGCAGTT CAGGGTTGGG TCTAAACGGA AGTGTGACCA CCAGTCAGAC AGCTGCTAGC 
GACAGCTACA ACAACTCTGA TTCTACAATG CTTACCCCTC CTTCCAGTCC ACCACCTCCT 
CCACCCAGGG AGGAACCTGC TTGTCTCCAA AACAAGAAGT CTCAGGTTTC CTGGGACAAC 
CTCCTTGAAG GCACAGAAGA ACCCAAATCT GAGACCATGC CCATTAAGCC CAGGACTCCA 
GTTAGTCCCC CTCAACCAAA ACAAAAACCA GTTACAGCTC CAGTGCCAAC CCCTAGAACC 
AATCCACCTG TTGTGATGAG GGTTAAGGAG CCCAATAAAC CACGTCGAGA AGAAGTGAGA 
AAGTCTTTTG TTGAGTGTGT AGACGAGATA CCATTTGCTG ATGATGTTGA AGACACATAC 
GATGACCGCA CTCCAGATGC AAGTGGTCTT GAAAAGTTTT ACACACCGCC GACTAGCAAG 
GTCAACCGGG ATAAGCCGCC TCTCCACTTG GCACTGGCTA TGGAAAATGG CAAGCCCAAT 
ATCCCTGGAG TTTCCCGAAC AGCAAAGGGT TCCCAGCATT TTTCTCCTGA AGCCAAGGAG 
ATCGCAGAGG AGCGAATGAG GGCACGGGAA AAGTCTGTGA AGAGCCAAGC TCTTAAAGAT 
GCAATGGCCA AGCAGCTTAC AAAGATGAAA GATTCTGAGG TTGCCAAGGG GGCTGTAGCA 
AAAGTAGCCT GGGATATTCC TGAAACAAAA GGAAAAAGCA AAAAGCAATC TAAAGCCCAG 
AAGGACTCTG CAGTGAAGGC CTTGGAGTCC AAAAAGCAAG CAGACACACT TCCAGACCGT 
TTCTTCTCTA CACCATCTAG TAAGGCCTTA GACAGCTCTG TCACATCTTC AGAGAGCTCC 
ACAGGGGGCA AAAGCAAGAA ACGCAGCTCA CTGTTTTCTC CACGCAAAAA CAAAAAAGAA 
AAGAAAGCCA AAAATGAAAG ACTGTCCAGC ACTGAAGAAA CGCCGCCCAA GCACAAGTCC 
CTTTGGAAAG CAGTGTTCTC CGGCTACAAG AAGGACAAAA AGAAGAAAGA TGACAAGTCA 
TGCCCAAGTA CACCCTCAAG CTCTACAACT GGAGACTCGG GGAAGAAGAA AGATTCACCC 
CTGGACAGGT CCTCAGATTT ACGTCTGAGG AGAAATCTAA GCTTTTCTGA GGACTCTGAT 
CTCTCCTGTG ACGATGTTCT GGAAAGATCC TCTCAGAAGT CCAAGGGTGA TCGTCATGTG 
GACCTGTTTG ACTTGGTGTC AGATTTGAAA AAAGAAGCCA TTAAAGAGGA GAAAGCAAAA 
GAGAAGGCTA AGGATAGGGC AAAGGAAAGG GGAAGAGTTA CTGAGAAGGG CAAGGAAAGG 
TCAAGAGAAA GGGAGAATGA AAAAAGAGTC AAAGAAAAGA TGGTGAAAAT GAAAGATGTG 
GAACGGGAGA AAGGAAAAGA GGAAGAGTCT GTTTATGTAC CTCATGCATT GGCGTTCAAG 
AGATCATATG CCACAAAGAA GACATACACG GAGGAGGAAC TGAACGCCAA GCTGACAAGA 
AGAGTACAGA AAGCTGCACG GAGGCAGGCC AAGCAGGAGG AGCTCAAGAG ACTTCACAGA 
GCTCAGATGA TTCAGAGGCA GCTGGAGCAG GTGGAAGAGA AACAGAGGCA GTTAGAGGAG 
AGAGGCGTCG CGGTGGAAAA GGCACTGCGA GGCGAAGCAG TTGAGCCCTA TGTCGGGACC 
CCTCGCCGAA GAGCCCTGTA CCTCTGCCCC TGCTGTGCCC CTGAAGGAAT GGGTAAAAAG 
GACGATCCGA AGCTGATGCA GGAATGGTTT AAACTGGTCC AGGAGAAGAA TGCCTTGGTA 
CGATATGAAT CCGAGCTGAT GATATTTGCT CGGGAGCTAG AGTTGGAGGA CAGACAGAGT 
CGACTGCAAC AGGAACTACG GGAACGCATG GCAATAGATG ATCATCTGAA GACAGAGGAG 
GAGCTGGCAG AGGAGAAGCA GATTCTGAAT GAGATGTTGG AGGTGGTGGA GCAGCGAGAC 
TCTCTGGTGG CCCTGCTGGA GGAACAGAGA CTCAGGGAGA AGGAAGAAGA TAAAGACCTG 
GAGGCGGTGA TGCTCTCCAA AGGATTCAAC CTCAACTGGG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291745.1
CDS	600..7364
Translation	MGDGGVNAVGEGVNQSHMLFDRFVQATTCKGTLKAFQELCDFLELKPNEYRVFYHKLKSKLNYWKAKALWAKLDKRASHKEYKKGRACANTKCLIIGAGPCGLRTAIELGFLGAKVVLLEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGIEIHVNVEFKGLIEPPEDQENERIGWRAEVHPRTHPVNELEFDVIIGADGRRNTLSGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILHDYADTEMLLSRANVDQKALLSYAREAADFSTNHQLPKLDFAINHYGQPDVAMFDFTCMYASENAALVRQRNGHKLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWAHGSSPLEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNISLHQVRPNQVRHLLDTGETRDLRVDLENVVNSSTPKLTRNESIVRSSKLLNWCQRQTEGYRGVSVSDLTTSWKSGLALCALIHRYRPDLIDFESLDEKDVEKNNQLAFDVAEREFGISPIMTGKEMSVVVEPDKLSMVMYLSQFYEMFKDTVPPGENQNLSPEEKAALIASTKSPISFLSKLGQSIAISRKRNPKDKKEKELDGLGKRRKTSQAGQSEDEELQRANRDDRPSIATALAERKIDSAAAANNNNKVKSMATQLLAKFEENAPTQSTGLKRQGDSMPDLGLILTPSPAAAPLNESVRLAPVPAWRKRRTQLQEQMSFRYKEKLKCQTLPITQEQASSGTEVLRSCPKKTILLSSSSPSSSSFSSSFPPHAQGSFRKEFPQNIGGSDVCFFCRKRVYVMERLSAEGKFFHRSCFKCDYCGTTLRLSSYAFDVEDGKFYCKPHYCYRLSGVAQRKRPAPAAAPANAKEPQVLAVSPNTVDAPGQAITTQTPAERRPSETEVNGVTEPSVAKRLKGTPERIELENYRLSMMREEELEEVPEETLAEHNLSSVLDKATDIEEGSSSSESDMEEEDEDAEAAGPSDLGGVPWKEAVELHAKLKGESDPGADDDGLHDGDGEMDEDEEEEEDEEDEDEEEEEESSEAGDYFPWERELQSGLWLENLANEEDVSVFKARNLQIQQAIHPVDPLDIQMESHQAEGVLEMPSVSPLGSQPSLNTQLTQPTSSTEPCEEDDDPEAEAGSPDFEPGTEIDQEDIPSDAEAEARSRCIDEVVTLPVDNDKTESQGQVFNTAEKTSANPRELIVDVVLSPIQKPALPIEEVHEVSPVVLVKSPGARFFPEPYLPDKVKQNIPPPQSPDVKTPHSPVAQIIALSPICSQPVPQPGTASPKSPVQPQPCACSPTGNPLSPICTQSQPCNEPPSPLSTSSPVRTQPVPAVTSTPLAKPASENRTNEHLKDSTPELKKTDLIEEFWLKSAEIRKSLGLTPLERSKTAVEKSIVKTPTPESSSPKSYTPEDLSEEQKPTFTGRSIIRRINITLEGQVISPVEPKSNGSEKKDLSSSSGLGLNGSVTTSQTAASDSYNNSDSTMLTPPSSPPPPPPREEPACLQNKKSQVSWDNLLEGTEEPKSETMPIKPRTPVSPPQPKQKPVTAPVPTPRTNPPVVMRVKEPNKPRREEVRKSFVECVDEIPFADDVEDTYDDRTPDASGLEKFYTPPTSKVNRDKPPLHLALAMENGKPNIPGVSRTAKGSQHFSPEAKEIAEERMRAREKSVKSQALKDAMAKQLTKMKDSEVAKGAVAKVAWDIPETKGKSKKQSKAQKDSAVKALESKKQADTLPDRFFSTPSSKALDSSVTSSESSTGGKSKKRSSLFSPRKNKKEKKAKNERLSSTEETPPKHKSLWKAVFSGYKKDKKKKDDKSCPSTPSSSTTGDSGKKKDSPLDRSSDLRLRRNLSFSEDSDLSCDDVLERSSQKSKGDRHVDLFDLVSDLKKEAIKEEKAKEKAKDRAKERGRVTEKGKERSRERENEKRVKEKMVKMKDVEREKGKEEESVYVPHALAFKRSYATKKTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQMIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPYVGTPRRRALYLCPCCAPEGMGKKDDPKLMQEWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAIDDHLKTEEELAEEKQILNEMLEVVEQRDSLVALLEEQRLREKEEDKDLEAVMLSKGFNLNWA

Target ORF information:

RefSeq Version	XM_009293470.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293470.2

ORF Insert Sequence:

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAGTCC 
ATCGTGCGCT CCAGTAAGCT GCTGAACTGG TGTCAGAGGC AGACGGAAGG CTACCGAGGT 
GTCAGCGTGT CTGATCTCAC CACGTCCTGG AAGAGTGGTT TGGCCCTGTG CGCGCTCATC 
CACAGATACA GACCAGACCT CATTGACTTT GAATCACTGG ACGAGAAAGA CGTGGAGAAG 
AACAACCAGC TGGCTTTCGA CGTGGCTGAA AGAGAGTTTG GGATTTCTCC CATCATGACC 
GGGAAGGAGA TGTCTGTTGT GGTGGAGCCA GATAAGCTCT CCATGGTGAT GTACCTCAGT 
CAGTTCTACG AGATGTTCAA GGACACGGTG CCACCTGGTG AAAACCAGAA TCTGAGCCCT 
GAGGAGAAGG CCGCACTGAT CGCCAGCACT AAATCACCCA TCTCATTCCT TAGTAAGCTA 
GGTCAAAGCA TCGCAATCTC CCGCAAAAGA AACCCCAAGG ATAAAAAAGA AAAGGAGCTG 
GATGGACTAG GGAAAAGACG AAAGACGAGC CAGGCTGGAC AATCGGAGGA CGAGGAGCTG 
CAGAGGGCTA ATCGAGACGA CAGGCCATCT ATAGCCACAG CTCTGGCAGA ACGTAAGATC 
GACTCCGCTG CAGCAGCCAA CAATAACAAC AAAGTGAAGT CGATGGCCAC GCAGCTGCTG 
GCCAAGTTTG AGGAGAACGC GCCGACACAG TCCACTGGGC TCAAACGACA GGGAGACTCT 
ATGCCCGATC TGGGCCTCAT ACTGACCCCT TCTCCAGCCG CTGCTCCTCT GAACGAGTCA 
GTTCGTCTGG CTCCAGTGCC TGCATGGAGG AAGAGGCGCA CACAGTTACA GGAGCAGATG 
AGTTTCCGCT ATAAAGAAAA ACTCAAGTGT CAAACTCTCC CCATCACACA GGAGCAGGCC 
AGCAGTGGGA CTGAGGTCTT GAGGAGCTGC CCTAAGAAAA CCATTCTTCT TTCTTCTTCC 
TCACCCTCAT CTTCCTCATT CTCTTCATCG TTTCCTCCAC ATGCACAGGG TTCCTTTCGA 
AAGGAGTTCC CTCAGAACAT CGGTGGCAGC GATGTGTGTT TCTTCTGCCG TAAACGGGTC 
TATGTGATGG AGCGTCTCAG CGCTGAAGGA AAGTTTTTCC ATCGCAGCTG CTTCAAGTGC 
GACTACTGCG GCACAACCCT CCGCCTGTCC TCATATGCCT TCGATGTTGA AGACGGGAAG 
TTTTATTGTA AACCACACTA CTGCTACCGA CTCTCTGGTG TGGCACAGAG AAAACGGCCT 
GCACCTGCTG CTGCTCCAGC AAACGCCAAG GAGCCCCAGG TGTTGGCGGT ATCCCCCAAC 
ACAGTGGACG CCCCAGGTCA AGCCATAACC ACTCAGACCC CTGCGGAGCG CCGGCCCTCA 
GAAACTGAGG TAAATGGTGT CACGGAGCCC AGCGTGGCCA AACGTCTGAA AGGGACTCCG 
GAAAGGATCG AGCTGGAGAA CTATCGTTTG TCAATGATGA GGGAGGAGGA GCTGGAGGAG 
GTGCCGGAGG AGACATTGGC GGAGCACAAC CTCAGCAGTG TGCTGGATAA AGCCACCGAC 
ATCGAGGAGG GATCCAGCAG CTCTGAGTCT GACATGGAGG AAGAGGATGA AGATGCAGAG 
GCGGCCGGTC CGTCTGATCT GGGTGGAGTT CCCTGGAAGG AGGCAGTAGA GCTCCATGCT 
AAGTTAAAGG GAGAAAGCGA CCCAGGAGCT GACGATGATG GCCTTCATGA CGGTGATGGA 
GAGATGGACG AGGATGAAGA AGAGGAGGAA GATGAGGAAG ATGAAGATGA GGAGGAAGAA 
GAGGAATCGA GCGAAGCGGG AGACTATTTC CCCTGGGAGA GGGAGCTTCA GTCAGGCCTT 
TGGCTGGAAA ATCTGGCAAA TGAAGAGGAT GTCAGTGTTT TTAAAGCTAG GAACCTGCAG 
ATCCAGCAAG CCATTCATCC AGTAGACCCT CTGGACATCC AGATGGAGAG CCATCAGGCT 
GAGGGGGTGT TGGAGATGCC CTCGGTGTCA CCCTTAGGCT CACAGCCCTC GCTCAACACC 
CAGCTCACAC AGCCCACCTC CTCCACAGAG CCCTGTGAAG AGGACGATGA CCCTGAAGCT 
GAAGCAGGCA GTCCTGACTT TGAGCCAGGC ACTGAAATAG ATCAAGAGGA CATTCCCTCA 
GATGCAGAGG CTGAAGCTCG TTCACGGTGC ATAGATGAAG TGGTGACACT TCCAGTGGAT 
AATGATAAAA CAGAAAGCCA GGGACAGGTT TTCAACACTG CAGAGAAAAC GTCTGCAAAT 
CCAAGGGAGT TGATTGTGGA CGTTGTGCTG TCTCCAATCC AAAAACCTGC TTTACCCATT 
GAGGAGGTGC ATGAAGTTTC TCCTGTAGTA CTAGTTAAAT CTCCTGGTGC ACGTTTCTTC 
CCTGAGCCCT ATCTGCCTGA TAAAGTCAAG CAGAACATTC CCCCGCCTCA GAGTCCTGAT 
GTTAAAACCC CTCATTCTCC TGTAGCTCAA ATCATTGCGC TGTCGCCCAT CTGTTCCCAG 
CCTGTTCCAC AACCAGGCAC AGCATCTCCC AAATCTCCAG TCCAACCACA GCCTTGTGCC 
TGTTCTCCAA CAGGCAATCC TTTGTCTCCG ATTTGTACCC AATCGCAACC CTGCAATGAG 
CCACCTTCAC CCCTATCCAC AAGCTCCCCC GTCAGGACTC AACCAGTCCC AGCAGTAACT 
TCTACTCCTT TAGCTAAACC TGCTTCCGAG AATAGGACTA ATGAACATTT GAAAGATTCA 
ACTCCTGAGC TTAAGAAAAC AGACCTCATT GAGGAGTTCT GGTTGAAGAG TGCAGAGATT 
AGAAAAAGTT TAGGGCTCAC TCCCCTGGAA CGAAGCAAAA CTGCGGTGGA GAAGAGCATT 
GTGAAAACTC CAACACCCGA ATCATCATCT CCTAAGTCTT ATACCCCAGA GGACTTGTCT 
GAGGAACAGA AGCCTACATT TACGGGCCGT TCTATCATAC GCAGGATCAA TATTACCTTG 
GAGGGTCAGG TTATATCTCC GGTGGAACCT AAAAGTAATG GTTCAGAAAA GAAGGACTTA 
AGCAGCAGTT CAGGGTTGGG TCTAAACGGA AGTGTGACCA CCAGTCAGAC AGCTGCTAGC 
GACAGCTACA ACAACTCTGA TTCTACAATG CTTACCCCTC CTTCCAGTCC ACCACCTCCT 
CCACCCAGGG AGGAACCTGC TTGTCTCCAA AACAAGAAGT CTCAGGTTTC CTGGGACAAC 
CTCCTTGAAG GCACAGAAGA ACCCAAATCT GAGACCATGC CCATTAAGCC CAGGACTCCA 
GTTAGTCCCC CTCAACCAAA ACAAAAACCA GTTACAGCTC CAGTGCCAAC CCCTAGAACC 
AATCCACCTG TTGTGATGAG GGTTAAGGAG CCCAATAAAC CACGTCGAGA AGAAGTGAGA 
AAGTCTTTTG TTGAGTGTGT AGACGAGATA CCATTTGCTG ATGATGTTGA AGACACATAC 
GATGACCGCA CTCCAGATGC AAGTGGTCTT GAAAAGTTTT ACACACCGCC GACTAGCAAG 
GTCAACCGGG ATAAGCCGCC TCTCCACTTG GCACTGGCTA TGGAAAATGG CAAGCCCAAT 
ATCCCTGGAG TTTCCCGAAC AGCAAAGGGT TCCCAGCATT TTTCTCCTGA AGCCAAGGAG 
ATCGCAGAGG AGCGAATGAG GGCACGGGAA AAGTCTGTGA AGAGCCAAGC TCTTAAAGAT 
GCAATGGCCA AGCAGCTTAC AAAGATGAAA GATTCTGAGG TTGCCAAGGG GGCTGTAGCA 
AAAGTAGCCT GGGATATTCC TGAAACAAAA GGAAAAAGCA AAAAGCAATC TAAAGCCCAG 
AAGGACTCTG CAGTGAAGGC CTTGGAGTCC AAAAAGCAAG CAGACACACT TCCAGACCGT 
TTCTTCTCTA CACCATCTAG TAAGGCCTTA GACAGCTCTG TCACATCTTC AGAGAGCTCC 
ACAGGGGGCA AAAGCAAGAA ACGCAGCTCA CTGTTTTCTC CACGCAAAAA CAAAAAAGAA 
AAGAAAGCCA AAAATGAAAG ACTGTCCAGC ACTGAAGAAA CGCCGCCCAA GCACAAGTCC 
CTTTGGAAAG CAGTGTTCTC CGGCTACAAG AAGGACAAAA AGAAGAAAGA TGACAAGTCA 
TGCCCAAGTA CACCCTCAAG CTCTACAACT GGAGACTCGG GGAAGAAGAA AGATTCACCC 
CTGGACAGGT CCTCAGATTT ACGTCTGAGG AGAAATCTAA GCTTTTCTGA GGACTCTGAT 
CTCTCCTGTG ACGATGTTCT GGAAAGATCC TCTCAGAAGT CCAAGGGTGA TCGTCATGTG 
GACCTGTTTG ACTTGGTGTC AGATTTGAAA AAAGAAGCCA TTAAAGAGGA GAAAGCAAAA 
GAGAAGGCTA AGGATAGGGC AAAGGAAAGG GGAAGAGTTA CTGAGAAGGG CAAGGAAAGG 
TCAAGAGAAA GGGAGAATGA AAAAAGAGTC AAAGAAAAGA TGGTGAAAAT GAAAGATGTG 
GAACGGGAGA AAGGAAAAGA GGAAGAGTCT GTTTATGTAC CTCATGCATT GGCGTTCAAG 
AGATCATATG CCACAAAGAA GACATACACG GAGGAGGAAC TGAACGCCAA GCTGACAAGA 
AGAGTACAGA AAGCTGCACG GAGGCAGGCC AAGCAGGAGG AGCTCAAGAG ACTTCACAGA 
GCTCAGATGA TTCAGAGGCA GCTGGAGCAG GTGGAAGAGA AACAGAGGCA GTTAGAGGAG 
AGAGGCGTCG CGGTGGAAAA GGCACTGCGA GGCGAAGCAG TTGAGCCCTA TGTCGGGACC 
CCTCGCCGAA GAGCCCTGTA CCTCTGCCCC TGCTGTGCCC CTGAAGGAAT GGGTAAAAAG 
GACGATCCGA AGCTGATGCA GGAATGGTTT AAACTGGTCC AGGAGAAGAA TGCCTTGGTA 
CGATATGAAT CCGAGCTGAT GATATTTGCT CGGGAGCTAG AGTTGGAGGA CAGACAGAGT 
CGACTGCAAC AGGAACTACG GGAACGCATG GCAATAGATG ATCATCTGAA GACAGAGGAG 
GAGCTGGCAG AGGAGAAGCA GATTCTGAAT GAGATGTTGG AGGTGGTGGA GCAGCGAGAC 
TCTCTGGTGG CCCTGCTGGA GGAACAGAGA CTCAGGGAGA AGGAAGAAGA TAAAGACCTG 
GAGGCGGTGA TGCTCTCCAA AGGATTCAAC CTCAACTGGG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3A ( XM_009293471.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009291746.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X3, mRNA.

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CloneID	ODa40702
Clone ID Related Accession (Same CDS sequence)	XM_009293471.2
Accession Version	XM_009293471.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein-methionine sulfoxide oxidase mical3a isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293471.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAGTCC 
ATCGTGCGCT CCAGTAAGCT GCTGAACTGG TGTCAGAGGC AGACGGAAGG CTACCGAGGT 
GTCAGCGTGT CTGATCTCAC CACGTCCTGG AAGAGTGGTT TGGCCCTGTG CGCGCTCATC 
CACAGATACA GACCAGACCT CATTGACTTT GAATCACTGG ACGAGAAAGA CGTGGAGAAG 
AACAACCAGC TGGCTTTCGA CGTGGCTGAA AGAGAGTTTG GGATTTCTCC CATCATGACC 
GGGAAGGAGA TGTCTGTTGT GGTGGAGCCA GATAAGCTCT CCATGGTGAT GTACCTCAGT 
CAGTTCTACG AGATGTTCAA GGACACGGTG CCACCTGGTG AAAACCAGAA TCTGAGCCCT 
GAGGAGAAGG CCGCACTGAT CGCCAGCACT AAATCACCCA TCTCATTCCT TAGTAAGCTA 
GGTCAAAGCA TCGCAATCTC CCGCAAAAGA AACCCCAAGG ATAAAAAAGA AAAGGAGCTG 
GATGGACTAG GGAAAAGACG AAAGACGAGC CAGGCTGGAC AATCGGAGGA CGAGGAGCTG 
CAGAGGGCTA ATCGAGACGA CAGGCCATCT ATAGCCACAG CTCTGGCAGA ACGTAAGATC 
GACTCCGCTG CAGCAGCCAA CAATAACAAC AAAGTGAAGT CGATGGCCAC GCAGCTGCTG 
GCCAAGTTTG AGGAGAACGC GCCGACACAG TCCACTGGGC TCAAACGACA GGGAGACTCT 
ATGCCCGATC TGGGCCTCAT ACTGACCCCT TCTCCAGCCG CTGCTCCTCT GAACGAGTCA 
GTTCGTCTGG CTCCAGTGCC TGCATGGAGG AAGAGGCGCA CACAGTTACA GGAGCAGATG 
AGTTTCCGCT ATAAAGAAAA ACTCAAGTGT CAAACTCTCC CCATCACACA GGAGCAGGCC 
AGCAGTGGGA CTGAGGTCTT GAGGAGCTGC CCTAAGAAAA CCATTCTTCT TTCTTCTTCC 
TCACCCTCAT CTTCCTCATT CTCTTCATCG TTTCCTCCAC ATGCACAGGG TTCCTTTCGA 
AAGGAGTTCC CTCAGAACAT CGGTGGCAGC GATGTGTGTT TCTTCTGCCG TAAACGGGTC 
TATGTGATGG AGCGTCTCAG CGCTGAAGGA AAGTTTTTCC ATCGCAGCTG CTTCAAGTGC 
GACTACTGCG GCACAACCCT CCGCCTGTCC TCATATGCCT TCGATGTTGA AGACGGGAAG 
TTTTATTGTA AACCACACTA CTGCTACCGA CTCTCTGGTG TGGCACAGAG AAAACGGCCT 
GCACCTGCTG CTGCTCCAGC AAACGCCAAG GAGCCCCAGG TGTTGGCGGT ATCCCCCAAC 
ACAGTGGACG CCCCAGGTCA AGCCATAACC ACTCAGACCC CTGCGGAGCG CCGGCCCTCA 
GAAACTGAGG TAAATGGTGT CACGGAGCCC AGCGTGGCCA AACGTCTGAA AGGGACTCCG 
GAAAGGATCG AGCTGGAGAA CTATCGTTTG TCAATGATGA GGGAGGAGGA GCTGGAGGAG 
GTGCCGGAGG AGACATTGGC GGAGCACAAC CTCAGCAGTG TGCTGGATAA AGCCACCGAC 
ATCGAGGAGG GATCCAGCAG CTCTGAGTCT GACATGGAGG AAGAGGATGA AGATGCAGAG 
GCGGCCGGTC CGTCTGATCT GGGTGGAGTT CCCTGGAAGG AGGCAGTAGA GCTCCATGCT 
AAGTTAAAGG GAGAAAGCGA CCCAGGAGCT GACGATGATG GCCTTCATGA CGGTGATGGA 
GAGATGGACG AGGATGAAGA AGAGGAGGAA GATGAGGAAG ATGAAGATGA GGAGGAAGAA 
GAGGAATCGA GCGAAGCCCC CTCCCACAAA TCCGTACGTC ACGAGGCTGT CAGAGCCTGG 
CTGGACTCGA TGTCTGGAGA GCCCTGTGAA GAGGACGATG ACCCTGAAGC TGAAGCAGGC 
AGTCCTGACT TTGAGCCAGG CACTGAAATA GATCAAGAGG ACATTCCCTC AGATGCAGAG 
GCTGAAGCTC GTTCACGGTG CATAGATGAA GTGGTGACAC TTCCAGTGGA TAATGATAAA 
ACAGAAAGCC AGGGACAGGT TTTCAACACT GCAGAGAAAA CGTCTGCAAA TCCAAGGGAG 
TTGATTGTGG ACGTTGTGCT GTCTCCAATC CAAAAACCTG CTTTACCCAT TGAGGAGGTG 
CATGAAGTTT CTCCTGTAGT ACTAGTTAAA TCTCCTGGTG CACGTTTCTT CCCTGAGCCC 
TATCTGCCTG ATAAAGTCAA GCAGAACATT CCCCCGCCTC AGAGTCCTGA TGTTAAAACC 
CCTCATTCTC CTGTAGCTCA AATCATTGCG CTGTCGCCCA TCTGTTCCCA GCCTGTTCCA 
CAACCAGGCA CAGCATCTCC CAAATCTCCA GTCCAACCAC AGCCTTGTGC CTGTTCTCCA 
ACAGGCAATC CTTTGTCTCC GATTTGTACC CAATCGCAAC CCTGCAATGA GCCACCTTCA 
CCCCTATCCA CAAGCTCCCC CGTCAGGACT CAACCAGTCC CAGCAGTAAC TTCTACTCCT 
TTAGCTAAAC CTGCTTCCGA GAATAGGACT AATGAACATT TGAAAGATTC AACTCCTGAG 
CTTAAGAAAA CAGACCTCAT TGAGGAGTTC TGGTTGAAGA GTGCAGAGAT TAGAAAAAGT 
TTAGGGCTCA CTCCCCTGGA ACGAAGCAAA ACTGCGGTGG AGAAGAGCAT TGTGAAAACT 
CCAACACCCG AATCATCATC TCCTAAGTCT TATACCCCAG AGGACTTGTC TGAGGAACAG 
AAGCCTACAT TTACGGGCCG TTCTATCATA CGCAGGATCA ATATTACCTT GGAGGGTCAG 
GTTATATCTC CGGTGGAACC TAAAAGTAAT GGTTCAGAAA AGAAGGACTT AAGCAGCAGT 
TCAGGGTTGG GTCTAAACGG AAGTGTGACC ACCAGTCAGA CAGCTGCTAG CGACAGCTAC 
AACAACTCTG ATTCTACAAT GCTTACCCCT CCTTCCAGTC CACCACCTCC TCCACCCAGG 
GAGGAACCTG CTTGTCTCCA AAACAAGAAG TCTCAGGTTT CCTGGGACAA CCTCCTTGAA 
GGCACAGAAG AACCCAAATC TGAGACCATG CCCATTAAGC CCAGGACTCC AGTTAGTCCC 
CCTCAACCAA AACAAAAACC AGTTACAGCT CCAGTGCCAA CCCCTAGAAC CAATCCACCT 
GTTGTGATGA GGGTTAAGGA GCCCAATAAA CCACGTCGAG AAGAAGTGAG AAAGTCTTTT 
GTTGAGTGTG TAGACGAGAT ACCATTTGCT GATGATGTTG AAGACACATA CGATGACCGC 
ACTCCAGATG CAAGTGGTCT TGAAAAGTTT TACACACCGC CGACTAGCAA GGTCAACCGG 
GATAAGCCGC CTCTCCACTT GGCACTGGCT ATGGAAAATG GCAAGCCCAA TATCCCTGGA 
GTTTCCCGAA CAGCAAAGGG TTCCCAGCAT TTTTCTCCTG AAGCCAAGGA GATCGCAGAG 
GAGCGAATGA GGGCACGGGA AAAGTCTGTG AAGAGCCAAG CTCTTAAAGA TGCAATGGCC 
AAGCAGCTTA CAAAGATGAA AGATTCTGAG GTTGCCAAGG GGGCTGTAGC AAAAGTAGCC 
TGGGATATTC CTGAAACAAA AGGAAAAAGC AAAAAGCAAT CTAAAGCCCA GAAGGACTCT 
GCAGTGAAGG CCTTGGAGTC CAAAAAGCAA GCAGACACAC TTCCAGACCG TTTCTTCTCT 
ACACCATCTA GTAAGGCCTT AGACAGCTCT GTCACATCTT CAGAGAGCTC CACAGGGGGC 
AAAAGCAAGA AACGCAGCTC ACTGTTTTCT CCACGCAAAA ACAAAAAAGA AAAGAAAGCC 
AAAAATGAAA GACTGTCCAG CACTGAAGAA ACGCCGCCCA AGCACAAGTC CCTTTGGAAA 
GCAGTGTTCT CCGGCTACAA GAAGGACAAA AAGAAGAAAG ATGACAAGTC ATGCCCAAGT 
ACACCCTCAA GCTCTACAAC TGGAGACTCG GGGAAGAAGA AAGATTCACC CCTGGACAGG 
TCCTCAGATT TACGTCTGAG GAGAAATCTA AGCTTTTCTG AGGACTCTGA TCTCTCCTGT 
GACGATGTTC TGGAAAGATC CTCTCAGAAG TCCAAGGGTG ATCGTCATGT GGACCTGTTT 
GACTTGGTGT CAGATTTGAA AAAAGAAGCC ATTAAAGAGG AGAAAGCAAA AGAGAAGGCT 
AAGGATAGGG CAAAGGAAAG GGGAAGAGTT ACTGAGAAGG GCAAGGAAAG GTCAAGAGAA 
AGGGAGAATG AAAAAAGAGT CAAAGAAAAG ATGGTGAAAA TGAAAGATGT GGAACGGGAG 
AAAGGAAAAG AGGAAGAGTC TGTTTATGTA CCTCATGCAT TGGCGTTCAA GAGATCATAT 
GCCACAAAGA AGACATACAC GGAGGAGGAA CTGAACGCCA AGCTGACAAG AAGAGTACAG 
AAAGCTGCAC GGAGGCAGGC CAAGCAGGAG GAGCTCAAGA GACTTCACAG AGCTCAGATG 
ATTCAGAGGC AGCTGGAGCA GGTGGAAGAG AAACAGAGGC AGTTAGAGGA GAGAGGCGTC 
GCGGTGGAAA AGGCACTGCG AGGCGAAGCA GTTGAGCCCT ATGTCGGGAC CCCTCGCCGA 
AGAGCCCTGT ACCTCTGCCC CTGCTGTGCC CCTGAAGGAA TGGGTAAAAA GGACGATCCG 
AAGCTGATGC AGGAATGGTT TAAACTGGTC CAGGAGAAGA ATGCCTTGGT ACGATATGAA 
TCCGAGCTGA TGATATTTGC TCGGGAGCTA GAGTTGGAGG ACAGACAGAG TCGACTGCAA 
CAGGAACTAC GGGAACGCAT GGCAATAGAT GATCATCTGA AGACAGAGGA GGAGCTGGCA 
GAGGAGAAGC AGATTCTGAA TGAGATGTTG GAGGTGGTGG AGCAGCGAGA CTCTCTGGTG 
GCCCTGCTGG AGGAACAGAG ACTCAGGGAG AAGGAAGAAG ATAAAGACCT GGAGGCGGTG 
ATGCTCTCCA AAGGATTCAA CCTCAACTGG GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291746.1
CDS	599..7174
Translation	MGDGGVNAVGEGVNQSHMLFDRFVQATTCKGTLKAFQELCDFLELKPNEYRVFYHKLKSKLNYWKAKALWAKLDKRASHKEYKKGRACANTKCLIIGAGPCGLRTAIELGFLGAKVVLLEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGIEIHVNVEFKGLIEPPEDQENERIGWRAEVHPRTHPVNELEFDVIIGADGRRNTLSGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILHDYADTEMLLSRANVDQKALLSYAREAADFSTNHQLPKLDFAINHYGQPDVAMFDFTCMYASENAALVRQRNGHKLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWAHGSSPLEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNISLHQVRPNQVRHLLDTGETRDLRVDLENVVNSSTPKLTRNESIVRSSKLLNWCQRQTEGYRGVSVSDLTTSWKSGLALCALIHRYRPDLIDFESLDEKDVEKNNQLAFDVAEREFGISPIMTGKEMSVVVEPDKLSMVMYLSQFYEMFKDTVPPGENQNLSPEEKAALIASTKSPISFLSKLGQSIAISRKRNPKDKKEKELDGLGKRRKTSQAGQSEDEELQRANRDDRPSIATALAERKIDSAAAANNNNKVKSMATQLLAKFEENAPTQSTGLKRQGDSMPDLGLILTPSPAAAPLNESVRLAPVPAWRKRRTQLQEQMSFRYKEKLKCQTLPITQEQASSGTEVLRSCPKKTILLSSSSPSSSSFSSSFPPHAQGSFRKEFPQNIGGSDVCFFCRKRVYVMERLSAEGKFFHRSCFKCDYCGTTLRLSSYAFDVEDGKFYCKPHYCYRLSGVAQRKRPAPAAAPANAKEPQVLAVSPNTVDAPGQAITTQTPAERRPSETEVNGVTEPSVAKRLKGTPERIELENYRLSMMREEELEEVPEETLAEHNLSSVLDKATDIEEGSSSSESDMEEEDEDAEAAGPSDLGGVPWKEAVELHAKLKGESDPGADDDGLHDGDGEMDEDEEEEEDEEDEDEEEEEESSEAPSHKSVRHEAVRAWLDSMSGEPCEEDDDPEAEAGSPDFEPGTEIDQEDIPSDAEAEARSRCIDEVVTLPVDNDKTESQGQVFNTAEKTSANPRELIVDVVLSPIQKPALPIEEVHEVSPVVLVKSPGARFFPEPYLPDKVKQNIPPPQSPDVKTPHSPVAQIIALSPICSQPVPQPGTASPKSPVQPQPCACSPTGNPLSPICTQSQPCNEPPSPLSTSSPVRTQPVPAVTSTPLAKPASENRTNEHLKDSTPELKKTDLIEEFWLKSAEIRKSLGLTPLERSKTAVEKSIVKTPTPESSSPKSYTPEDLSEEQKPTFTGRSIIRRINITLEGQVISPVEPKSNGSEKKDLSSSSGLGLNGSVTTSQTAASDSYNNSDSTMLTPPSSPPPPPPREEPACLQNKKSQVSWDNLLEGTEEPKSETMPIKPRTPVSPPQPKQKPVTAPVPTPRTNPPVVMRVKEPNKPRREEVRKSFVECVDEIPFADDVEDTYDDRTPDASGLEKFYTPPTSKVNRDKPPLHLALAMENGKPNIPGVSRTAKGSQHFSPEAKEIAEERMRAREKSVKSQALKDAMAKQLTKMKDSEVAKGAVAKVAWDIPETKGKSKKQSKAQKDSAVKALESKKQADTLPDRFFSTPSSKALDSSVTSSESSTGGKSKKRSSLFSPRKNKKEKKAKNERLSSTEETPPKHKSLWKAVFSGYKKDKKKKDDKSCPSTPSSSTTGDSGKKKDSPLDRSSDLRLRRNLSFSEDSDLSCDDVLERSSQKSKGDRHVDLFDLVSDLKKEAIKEEKAKEKAKDRAKERGRVTEKGKERSRERENEKRVKEKMVKMKDVEREKGKEEESVYVPHALAFKRSYATKKTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQMIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPYVGTPRRRALYLCPCCAPEGMGKKDDPKLMQEWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAIDDHLKTEEELAEEKQILNEMLEVVEQRDSLVALLEEQRLREKEEDKDLEAVMLSKGFNLNWA

Target ORF information:

RefSeq Version	XM_009293471.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293471.2

ORF Insert Sequence:

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAGTCC 
ATCGTGCGCT CCAGTAAGCT GCTGAACTGG TGTCAGAGGC AGACGGAAGG CTACCGAGGT 
GTCAGCGTGT CTGATCTCAC CACGTCCTGG AAGAGTGGTT TGGCCCTGTG CGCGCTCATC 
CACAGATACA GACCAGACCT CATTGACTTT GAATCACTGG ACGAGAAAGA CGTGGAGAAG 
AACAACCAGC TGGCTTTCGA CGTGGCTGAA AGAGAGTTTG GGATTTCTCC CATCATGACC 
GGGAAGGAGA TGTCTGTTGT GGTGGAGCCA GATAAGCTCT CCATGGTGAT GTACCTCAGT 
CAGTTCTACG AGATGTTCAA GGACACGGTG CCACCTGGTG AAAACCAGAA TCTGAGCCCT 
GAGGAGAAGG CCGCACTGAT CGCCAGCACT AAATCACCCA TCTCATTCCT TAGTAAGCTA 
GGTCAAAGCA TCGCAATCTC CCGCAAAAGA AACCCCAAGG ATAAAAAAGA AAAGGAGCTG 
GATGGACTAG GGAAAAGACG AAAGACGAGC CAGGCTGGAC AATCGGAGGA CGAGGAGCTG 
CAGAGGGCTA ATCGAGACGA CAGGCCATCT ATAGCCACAG CTCTGGCAGA ACGTAAGATC 
GACTCCGCTG CAGCAGCCAA CAATAACAAC AAAGTGAAGT CGATGGCCAC GCAGCTGCTG 
GCCAAGTTTG AGGAGAACGC GCCGACACAG TCCACTGGGC TCAAACGACA GGGAGACTCT 
ATGCCCGATC TGGGCCTCAT ACTGACCCCT TCTCCAGCCG CTGCTCCTCT GAACGAGTCA 
GTTCGTCTGG CTCCAGTGCC TGCATGGAGG AAGAGGCGCA CACAGTTACA GGAGCAGATG 
AGTTTCCGCT ATAAAGAAAA ACTCAAGTGT CAAACTCTCC CCATCACACA GGAGCAGGCC 
AGCAGTGGGA CTGAGGTCTT GAGGAGCTGC CCTAAGAAAA CCATTCTTCT TTCTTCTTCC 
TCACCCTCAT CTTCCTCATT CTCTTCATCG TTTCCTCCAC ATGCACAGGG TTCCTTTCGA 
AAGGAGTTCC CTCAGAACAT CGGTGGCAGC GATGTGTGTT TCTTCTGCCG TAAACGGGTC 
TATGTGATGG AGCGTCTCAG CGCTGAAGGA AAGTTTTTCC ATCGCAGCTG CTTCAAGTGC 
GACTACTGCG GCACAACCCT CCGCCTGTCC TCATATGCCT TCGATGTTGA AGACGGGAAG 
TTTTATTGTA AACCACACTA CTGCTACCGA CTCTCTGGTG TGGCACAGAG AAAACGGCCT 
GCACCTGCTG CTGCTCCAGC AAACGCCAAG GAGCCCCAGG TGTTGGCGGT ATCCCCCAAC 
ACAGTGGACG CCCCAGGTCA AGCCATAACC ACTCAGACCC CTGCGGAGCG CCGGCCCTCA 
GAAACTGAGG TAAATGGTGT CACGGAGCCC AGCGTGGCCA AACGTCTGAA AGGGACTCCG 
GAAAGGATCG AGCTGGAGAA CTATCGTTTG TCAATGATGA GGGAGGAGGA GCTGGAGGAG 
GTGCCGGAGG AGACATTGGC GGAGCACAAC CTCAGCAGTG TGCTGGATAA AGCCACCGAC 
ATCGAGGAGG GATCCAGCAG CTCTGAGTCT GACATGGAGG AAGAGGATGA AGATGCAGAG 
GCGGCCGGTC CGTCTGATCT GGGTGGAGTT CCCTGGAAGG AGGCAGTAGA GCTCCATGCT 
AAGTTAAAGG GAGAAAGCGA CCCAGGAGCT GACGATGATG GCCTTCATGA CGGTGATGGA 
GAGATGGACG AGGATGAAGA AGAGGAGGAA GATGAGGAAG ATGAAGATGA GGAGGAAGAA 
GAGGAATCGA GCGAAGCCCC CTCCCACAAA TCCGTACGTC ACGAGGCTGT CAGAGCCTGG 
CTGGACTCGA TGTCTGGAGA GCCCTGTGAA GAGGACGATG ACCCTGAAGC TGAAGCAGGC 
AGTCCTGACT TTGAGCCAGG CACTGAAATA GATCAAGAGG ACATTCCCTC AGATGCAGAG 
GCTGAAGCTC GTTCACGGTG CATAGATGAA GTGGTGACAC TTCCAGTGGA TAATGATAAA 
ACAGAAAGCC AGGGACAGGT TTTCAACACT GCAGAGAAAA CGTCTGCAAA TCCAAGGGAG 
TTGATTGTGG ACGTTGTGCT GTCTCCAATC CAAAAACCTG CTTTACCCAT TGAGGAGGTG 
CATGAAGTTT CTCCTGTAGT ACTAGTTAAA TCTCCTGGTG CACGTTTCTT CCCTGAGCCC 
TATCTGCCTG ATAAAGTCAA GCAGAACATT CCCCCGCCTC AGAGTCCTGA TGTTAAAACC 
CCTCATTCTC CTGTAGCTCA AATCATTGCG CTGTCGCCCA TCTGTTCCCA GCCTGTTCCA 
CAACCAGGCA CAGCATCTCC CAAATCTCCA GTCCAACCAC AGCCTTGTGC CTGTTCTCCA 
ACAGGCAATC CTTTGTCTCC GATTTGTACC CAATCGCAAC CCTGCAATGA GCCACCTTCA 
CCCCTATCCA CAAGCTCCCC CGTCAGGACT CAACCAGTCC CAGCAGTAAC TTCTACTCCT 
TTAGCTAAAC CTGCTTCCGA GAATAGGACT AATGAACATT TGAAAGATTC AACTCCTGAG 
CTTAAGAAAA CAGACCTCAT TGAGGAGTTC TGGTTGAAGA GTGCAGAGAT TAGAAAAAGT 
TTAGGGCTCA CTCCCCTGGA ACGAAGCAAA ACTGCGGTGG AGAAGAGCAT TGTGAAAACT 
CCAACACCCG AATCATCATC TCCTAAGTCT TATACCCCAG AGGACTTGTC TGAGGAACAG 
AAGCCTACAT TTACGGGCCG TTCTATCATA CGCAGGATCA ATATTACCTT GGAGGGTCAG 
GTTATATCTC CGGTGGAACC TAAAAGTAAT GGTTCAGAAA AGAAGGACTT AAGCAGCAGT 
TCAGGGTTGG GTCTAAACGG AAGTGTGACC ACCAGTCAGA CAGCTGCTAG CGACAGCTAC 
AACAACTCTG ATTCTACAAT GCTTACCCCT CCTTCCAGTC CACCACCTCC TCCACCCAGG 
GAGGAACCTG CTTGTCTCCA AAACAAGAAG TCTCAGGTTT CCTGGGACAA CCTCCTTGAA 
GGCACAGAAG AACCCAAATC TGAGACCATG CCCATTAAGC CCAGGACTCC AGTTAGTCCC 
CCTCAACCAA AACAAAAACC AGTTACAGCT CCAGTGCCAA CCCCTAGAAC CAATCCACCT 
GTTGTGATGA GGGTTAAGGA GCCCAATAAA CCACGTCGAG AAGAAGTGAG AAAGTCTTTT 
GTTGAGTGTG TAGACGAGAT ACCATTTGCT GATGATGTTG AAGACACATA CGATGACCGC 
ACTCCAGATG CAAGTGGTCT TGAAAAGTTT TACACACCGC CGACTAGCAA GGTCAACCGG 
GATAAGCCGC CTCTCCACTT GGCACTGGCT ATGGAAAATG GCAAGCCCAA TATCCCTGGA 
GTTTCCCGAA CAGCAAAGGG TTCCCAGCAT TTTTCTCCTG AAGCCAAGGA GATCGCAGAG 
GAGCGAATGA GGGCACGGGA AAAGTCTGTG AAGAGCCAAG CTCTTAAAGA TGCAATGGCC 
AAGCAGCTTA CAAAGATGAA AGATTCTGAG GTTGCCAAGG GGGCTGTAGC AAAAGTAGCC 
TGGGATATTC CTGAAACAAA AGGAAAAAGC AAAAAGCAAT CTAAAGCCCA GAAGGACTCT 
GCAGTGAAGG CCTTGGAGTC CAAAAAGCAA GCAGACACAC TTCCAGACCG TTTCTTCTCT 
ACACCATCTA GTAAGGCCTT AGACAGCTCT GTCACATCTT CAGAGAGCTC CACAGGGGGC 
AAAAGCAAGA AACGCAGCTC ACTGTTTTCT CCACGCAAAA ACAAAAAAGA AAAGAAAGCC 
AAAAATGAAA GACTGTCCAG CACTGAAGAA ACGCCGCCCA AGCACAAGTC CCTTTGGAAA 
GCAGTGTTCT CCGGCTACAA GAAGGACAAA AAGAAGAAAG ATGACAAGTC ATGCCCAAGT 
ACACCCTCAA GCTCTACAAC TGGAGACTCG GGGAAGAAGA AAGATTCACC CCTGGACAGG 
TCCTCAGATT TACGTCTGAG GAGAAATCTA AGCTTTTCTG AGGACTCTGA TCTCTCCTGT 
GACGATGTTC TGGAAAGATC CTCTCAGAAG TCCAAGGGTG ATCGTCATGT GGACCTGTTT 
GACTTGGTGT CAGATTTGAA AAAAGAAGCC ATTAAAGAGG AGAAAGCAAA AGAGAAGGCT 
AAGGATAGGG CAAAGGAAAG GGGAAGAGTT ACTGAGAAGG GCAAGGAAAG GTCAAGAGAA 
AGGGAGAATG AAAAAAGAGT CAAAGAAAAG ATGGTGAAAA TGAAAGATGT GGAACGGGAG 
AAAGGAAAAG AGGAAGAGTC TGTTTATGTA CCTCATGCAT TGGCGTTCAA GAGATCATAT 
GCCACAAAGA AGACATACAC GGAGGAGGAA CTGAACGCCA AGCTGACAAG AAGAGTACAG 
AAAGCTGCAC GGAGGCAGGC CAAGCAGGAG GAGCTCAAGA GACTTCACAG AGCTCAGATG 
ATTCAGAGGC AGCTGGAGCA GGTGGAAGAG AAACAGAGGC AGTTAGAGGA GAGAGGCGTC 
GCGGTGGAAA AGGCACTGCG AGGCGAAGCA GTTGAGCCCT ATGTCGGGAC CCCTCGCCGA 
AGAGCCCTGT ACCTCTGCCC CTGCTGTGCC CCTGAAGGAA TGGGTAAAAA GGACGATCCG 
AAGCTGATGC AGGAATGGTT TAAACTGGTC CAGGAGAAGA ATGCCTTGGT ACGATATGAA 
TCCGAGCTGA TGATATTTGC TCGGGAGCTA GAGTTGGAGG ACAGACAGAG TCGACTGCAA 
CAGGAACTAC GGGAACGCAT GGCAATAGAT GATCATCTGA AGACAGAGGA GGAGCTGGCA 
GAGGAGAAGC AGATTCTGAA TGAGATGTTG GAGGTGGTGG AGCAGCGAGA CTCTCTGGTG 
GCCCTGCTGG AGGAACAGAG ACTCAGGGAG AAGGAAGAAG ATAAAGACCT GGAGGCGGTG 
ATGCTCTCCA AAGGATTCAA CCTCAACTGG GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3A ( XM_009293472.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009291747.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X4, mRNA.

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CloneID	ODa40703
Clone ID Related Accession (Same CDS sequence)	XM_009293472.2
Accession Version	XM_009293472.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein-methionine sulfoxide oxidase mical3a isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293472.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAGTCC 
ATCGTGCGCT CCAGTAAGCT GCTGAACTGG TGTCAGAGGC AGACGGAAGG CTACCGAGGT 
GTCAGCGTGT CTGATCTCAC CACGTCCTGG AAGAGTGGTT TGGCCCTGTG CGCGCTCATC 
CACAGATACA GACCAGACCT CATTGACTTT GAATCACTGG ACGAGAAAGA CGTGGAGAAG 
AACAACCAGC TGGCTTTCGA CGTGGCTGAA AGAGAGTTTG GGATTTCTCC CATCATGACC 
GGGAAGGAGA TGTCTGTTGT GGTGGAGCCA GATAAGCTCT CCATGGTGAT GTACCTCAGT 
CAGTTCTACG AGATGTTCAA GGACACGGTG CCACCTGGTG AAAACCAGAA TCTGAGCCCT 
GAGGAGAAGG CCGCACTGAT CGCCAGCACT AAATCACCCA TCTCATTCCT TAGTAAGCTA 
GGTCAAAGCA TCGCAATCTC CCGCAAAAGA AACCCCAAGG ATAAAAAAGA AAAGGAGCTG 
GATGGACTAG GGAAAAGACG AAAGACGAGC CAGGCTGGAC AATCGGAGGA CGAGGAGCTG 
CAGAGGGCTA ATCGAGACGA CAGGCCATCT ATAGCCACAG CTCTGGCAGA ACGTAAGATC 
GACTCCGCTG CAGCAGCCAA CAATAACAAC AAAGTGAAGT CGATGGCCAC GCAGCTGCTG 
GCCAAGTTTG AGGAGAACGC GCCGACACAG TCCACTGGGC TCAAACGACA GGGAGACTCT 
ATGCCCGATC TGGGCCTCAT ACTGACCCCT TCTCCAGCCG CTGCTCCTCT GAACGAGTCA 
GTTCGTCTGG CTCCAGTGCC TGCATGGAGG AAGAGGCGCA CACAGTTACA GGAGCAGATG 
AGTTTCCGCT ATAAAGAAAA ACTCAAGTGT CAAACTCTCC CCATCACACA GGAGCAGGCC 
AGCAGTGGGA CTGAGGTCTT GAGGAGCTGC CCTAAGAAAA CCATTCTTCT TTCTTCTTCC 
TCACCCTCAT CTTCCTCATT CTCTTCATCG TTTCCTCCAC ATGCACAGGG TTCCTTTCGA 
AAGGAGTTCC CTCAGAACAT CGGTGGCAGC GATGTGTGTT TCTTCTGCCG TAAACGGGTC 
TATGTGATGG AGCGTCTCAG CGCTGAAGGA AAGTTTTTCC ATCGCAGCTG CTTCAAGTGC 
GACTACTGCG GCACAACCCT CCGCCTGTCC TCATATGCCT TCGATGTTGA AGACGGGAAG 
TTTTATTGTA AACCACACTA CTGCTACCGA CTCTCTGGTG TGGCACAGAG AAAACGGCCT 
GCACCTGCTG CTGCTCCAGC AAACGCCAAG GAGCCCCAGG TGTTGGCGGT ATCCCCCAAC 
ACAGTGGACG CCCCAGGTCA AGCCATAACC ACTCAGACCC CTGCGGAGCG CCGGCCCTCA 
GAAACTGAGG TAAATGGTGT CACGGAGCCC AGCGTGGCCA AACGTCTGAA AGGGACTCCG 
GAAAGGATCG AGCTGGAGAA CTATCGTTTG TCAATGATGA GGGAGGAGGA GCTGGAGGAG 
GTGCCGGAGG AGACATTGGC GGAGCACAAC CTCAGCAGTG TGCTGGATAA AGCCACCGAC 
ATCGAGGAGG GATCCAGCAG CTCTGAGTCT GACATGGAGG AAGAGGATGA AGATGCAGAG 
GCGGCCGGTC CGTCTGATCT GGGTGGAGTT CCCTGGAAGG AGGCAGTAGA GCTCCATGCT 
AAGTTAAAGG GAGAAAGCGA CCCAGGAGCT GACGATGATG GCCTTCATGA CGGTGATGGA 
GAGATGGACG AGGATGAAGA AGAGGAGGAA GATGAGGAAG ATGAAGATGA GGAGGAAGAA 
GAGGAATCGA GCGAAGAGCC CTGTGAAGAG GACGATGACC CTGAAGCTGA AGCAGGCAGT 
CCTGACTTTG AGCCAGGCAC TGAAATAGAT CAAGAGGACA TTCCCTCAGA TGCAGAGGCT 
GAAGCTCGTT CACGGTGCAT AGATGAAGTG GTGACACTTC CAGTGGATAA TGATAAAACA 
GAAAGCCAGG GACAGGTTTT CAACACTGCA GAGAAAACGT CTGCAAATCC AAGGGAGTTG 
ATTGTGGACG TTGTGCTGTC TCCAATCCAA AAACCTGCTT TACCCATTGA GGAGGTGCAT 
GAAGTTTCTC CTGTAGTACT AGTTAAATCT CCTGGTGCAC GTTTCTTCCC TGAGCCCTAT 
CTGCCTGATA AAGTCAAGCA GAACATTCCC CCGCCTCAGA GTCCTGATGT TAAAACCCCT 
CATTCTCCTG TAGCTCAAAT CATTGCGCTG TCGCCCATCT GTTCCCAGCC TGTTCCACAA 
CCAGGCACAG CATCTCCCAA ATCTCCAGTC CAACCACAGC CTTGTGCCTG TTCTCCAACA 
GGCAATCCTT TGTCTCCGAT TTGTACCCAA TCGCAACCCT GCAATGAGCC ACCTTCACCC 
CTATCCACAA GCTCCCCCGT CAGGACTCAA CCAGTCCCAG CAGTAACTTC TACTCCTTTA 
GCTAAACCTG CTTCCGAGAA TAGGACTAAT GAACATTTGA AAGATTCAAC TCCTGAGCTT 
AAGAAAACAG ACCTCATTGA GGAGTTCTGG TTGAAGAGTG CAGAGATTAG AAAAAGTTTA 
GGGCTCACTC CCCTGGAACG AAGCAAAACT GCGGTGGAGA AGAGCATTGT GAAAACTCCA 
ACACCCGAAT CATCATCTCC TAAGTCTTAT ACCCCAGAGG ACTTGTCTGA GGAACAGAAG 
CCTACATTTA CGGGCCGTTC TATCATACGC AGGATCAATA TTACCTTGGA GGGTCAGGTT 
ATATCTCCGG TGGAACCTAA AAGTAATGGT TCAGAAAAGA AGGACTTAAG CAGCAGTTCA 
GGGTTGGGTC TAAACGGAAG TGTGACCACC AGTCAGACAG CTGCTAGCGA CAGCTACAAC 
AACTCTGATT CTACAATGCT TACCCCTCCT TCCAGTCCAC CACCTCCTCC ACCCAGGGAG 
GAACCTGCTT GTCTCCAAAA CAAGAAGTCT CAGGTTTCCT GGGACAACCT CCTTGAAGGC 
ACAGAAGAAC CCAAATCTGA GACCATGCCC ATTAAGCCCA GGACTCCAGT TAGTCCCCCT 
CAACCAAAAC AAAAACCAGT TACAGCTCCA GTGCCAACCC CTAGAACCAA TCCACCTGTT 
GTGATGAGGG TTAAGGAGCC CAATAAACCA CGTCGAGAAG AAGTGAGAAA GTCTTTTGTT 
GAGTGTGTAG ACGAGATACC ATTTGCTGAT GATGTTGAAG ACACATACGA TGACCGCACT 
CCAGATGCAA GTGGTCTTGA AAAGTTTTAC ACACCGCCGA CTAGCAAGGT CAACCGGGAT 
AAGCCGCCTC TCCACTTGGC ACTGGCTATG GAAAATGGCA AGCCCAATAT CCCTGGAGTT 
TCCCGAACAG CAAAGGGTTC CCAGCATTTT TCTCCTGAAG CCAAGGAGAT CGCAGAGGAG 
CGAATGAGGG CACGGGAAAA GTCTGTGAAG AGCCAAGCTC TTAAAGATGC AATGGCCAAG 
CAGCTTACAA AGATGAAAGA TTCTGAGGTT GCCAAGGGGG CTGTAGCAAA AGTAGCCTGG 
GATATTCCTG AAACAAAAGG AAAAAGCAAA AAGCAATCTA AAGCCCAGAA GGACTCTGCA 
GTGAAGGCCT TGGAGTCCAA AAAGCAAGCA GACACACTTC CAGACCGTTT CTTCTCTACA 
CCATCTAGTA AGGCCTTAGA CAGCTCTGTC ACATCTTCAG AGAGCTCCAC AGGGGGCAAA 
AGCAAGAAAC GCAGCTCACT GTTTTCTCCA CGCAAAAACA AAAAAGAAAA GAAAGCCAAA 
AATGAAAGAC TGTCCAGCAC TGAAGAAACG CCGCCCAAGC ACAAGTCCCT TTGGAAAGCA 
GTGTTCTCCG GCTACAAGAA GGACAAAAAG AAGAAAGATG ACAAGTCATG CCCAAGTACA 
CCCTCAAGCT CTACAACTGG AGACTCGGGG AAGAAGAAAG ATTCACCCCT GGACAGGTCC 
TCAGATTTAC GTCTGAGGAG AAATCTAAGC TTTTCTGAGG ACTCTGATCT CTCCTGTGAC 
GATGTTCTGG AAAGATCCTC TCAGAAGTCC AAGGGTGATC GTCATGTGGA CCTGTTTGAC 
TTGGTGTCAG ATTTGAAAAA AGAAGCCATT AAAGAGGAGA AAGCAAAAGA GAAGGCTAAG 
GATAGGGCAA AGGAAAGGGG AAGAGTTACT GAGAAGGGCA AGGAAAGGTC AAGAGAAAGG 
GAGAATGAAA AAAGAGTCAA AGAAAAGATG GTGAAAATGA AAGATGTGGA ACGGGAGAAA 
GGAAAAGAGG AAGAGTCTGT TTATGTACCT CATGCATTGG CGTTCAAGAG ATCATATGCC 
ACAAAGAAGA CATACACGGA GGAGGAACTG AACGCCAAGC TGACAAGAAG AGTACAGAAA 
GCTGCACGGA GGCAGGCCAA GCAGGAGGAG CTCAAGAGAC TTCACAGAGC TCAGATGATT 
CAGAGGCAGC TGGAGCAGGT GGAAGAGAAA CAGAGGCAGT TAGAGGAGAG AGGCGTCGCG 
GTGGAAAAGG CACTGCGAGG CGAAGCAGTT GAGCCCTATG TCGGGACCCC TCGCCGAAGA 
GCCCTGTACC TCTGCCCCTG CTGTGCCCCT GAAGGAATGG GTAAAAAGGA CGATCCGAAG 
CTGATGCAGG AATGGTTTAA ACTGGTCCAG GAGAAGAATG CCTTGGTACG ATATGAATCC 
GAGCTGATGA TATTTGCTCG GGAGCTAGAG TTGGAGGACA GACAGAGTCG ACTGCAACAG 
GAACTACGGG AACGCATGGC AATAGATGAT CATCTGAAGA CAGAGGAGGA GCTGGCAGAG 
GAGAAGCAGA TTCTGAATGA GATGTTGGAG GTGGTGGAGC AGCGAGACTC TCTGGTGGCC 
CTGCTGGAGG AACAGAGACT CAGGGAGAAG GAAGAAGATA AAGACCTGGA GGCGGTGATG 
CTCTCCAAAG GATTCAACCT CAACTGGGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291747.1
CDS	598..7110
Translation	MGDGGVNAVGEGVNQSHMLFDRFVQATTCKGTLKAFQELCDFLELKPNEYRVFYHKLKSKLNYWKAKALWAKLDKRASHKEYKKGRACANTKCLIIGAGPCGLRTAIELGFLGAKVVLLEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGIEIHVNVEFKGLIEPPEDQENERIGWRAEVHPRTHPVNELEFDVIIGADGRRNTLSGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILHDYADTEMLLSRANVDQKALLSYAREAADFSTNHQLPKLDFAINHYGQPDVAMFDFTCMYASENAALVRQRNGHKLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWAHGSSPLEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNISLHQVRPNQVRHLLDTGETRDLRVDLENVVNSSTPKLTRNESIVRSSKLLNWCQRQTEGYRGVSVSDLTTSWKSGLALCALIHRYRPDLIDFESLDEKDVEKNNQLAFDVAEREFGISPIMTGKEMSVVVEPDKLSMVMYLSQFYEMFKDTVPPGENQNLSPEEKAALIASTKSPISFLSKLGQSIAISRKRNPKDKKEKELDGLGKRRKTSQAGQSEDEELQRANRDDRPSIATALAERKIDSAAAANNNNKVKSMATQLLAKFEENAPTQSTGLKRQGDSMPDLGLILTPSPAAAPLNESVRLAPVPAWRKRRTQLQEQMSFRYKEKLKCQTLPITQEQASSGTEVLRSCPKKTILLSSSSPSSSSFSSSFPPHAQGSFRKEFPQNIGGSDVCFFCRKRVYVMERLSAEGKFFHRSCFKCDYCGTTLRLSSYAFDVEDGKFYCKPHYCYRLSGVAQRKRPAPAAAPANAKEPQVLAVSPNTVDAPGQAITTQTPAERRPSETEVNGVTEPSVAKRLKGTPERIELENYRLSMMREEELEEVPEETLAEHNLSSVLDKATDIEEGSSSSESDMEEEDEDAEAAGPSDLGGVPWKEAVELHAKLKGESDPGADDDGLHDGDGEMDEDEEEEEDEEDEDEEEEEESSEEPCEEDDDPEAEAGSPDFEPGTEIDQEDIPSDAEAEARSRCIDEVVTLPVDNDKTESQGQVFNTAEKTSANPRELIVDVVLSPIQKPALPIEEVHEVSPVVLVKSPGARFFPEPYLPDKVKQNIPPPQSPDVKTPHSPVAQIIALSPICSQPVPQPGTASPKSPVQPQPCACSPTGNPLSPICTQSQPCNEPPSPLSTSSPVRTQPVPAVTSTPLAKPASENRTNEHLKDSTPELKKTDLIEEFWLKSAEIRKSLGLTPLERSKTAVEKSIVKTPTPESSSPKSYTPEDLSEEQKPTFTGRSIIRRINITLEGQVISPVEPKSNGSEKKDLSSSSGLGLNGSVTTSQTAASDSYNNSDSTMLTPPSSPPPPPPREEPACLQNKKSQVSWDNLLEGTEEPKSETMPIKPRTPVSPPQPKQKPVTAPVPTPRTNPPVVMRVKEPNKPRREEVRKSFVECVDEIPFADDVEDTYDDRTPDASGLEKFYTPPTSKVNRDKPPLHLALAMENGKPNIPGVSRTAKGSQHFSPEAKEIAEERMRAREKSVKSQALKDAMAKQLTKMKDSEVAKGAVAKVAWDIPETKGKSKKQSKAQKDSAVKALESKKQADTLPDRFFSTPSSKALDSSVTSSESSTGGKSKKRSSLFSPRKNKKEKKAKNERLSSTEETPPKHKSLWKAVFSGYKKDKKKKDDKSCPSTPSSSTTGDSGKKKDSPLDRSSDLRLRRNLSFSEDSDLSCDDVLERSSQKSKGDRHVDLFDLVSDLKKEAIKEEKAKEKAKDRAKERGRVTEKGKERSRERENEKRVKEKMVKMKDVEREKGKEEESVYVPHALAFKRSYATKKTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQMIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPYVGTPRRRALYLCPCCAPEGMGKKDDPKLMQEWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAIDDHLKTEEELAEEKQILNEMLEVVEQRDSLVALLEEQRLREKEEDKDLEAVMLSKGFNLNWA

Target ORF information:

RefSeq Version	XM_009293472.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293472.2

ORF Insert Sequence:

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAGTCC 
ATCGTGCGCT CCAGTAAGCT GCTGAACTGG TGTCAGAGGC AGACGGAAGG CTACCGAGGT 
GTCAGCGTGT CTGATCTCAC CACGTCCTGG AAGAGTGGTT TGGCCCTGTG CGCGCTCATC 
CACAGATACA GACCAGACCT CATTGACTTT GAATCACTGG ACGAGAAAGA CGTGGAGAAG 
AACAACCAGC TGGCTTTCGA CGTGGCTGAA AGAGAGTTTG GGATTTCTCC CATCATGACC 
GGGAAGGAGA TGTCTGTTGT GGTGGAGCCA GATAAGCTCT CCATGGTGAT GTACCTCAGT 
CAGTTCTACG AGATGTTCAA GGACACGGTG CCACCTGGTG AAAACCAGAA TCTGAGCCCT 
GAGGAGAAGG CCGCACTGAT CGCCAGCACT AAATCACCCA TCTCATTCCT TAGTAAGCTA 
GGTCAAAGCA TCGCAATCTC CCGCAAAAGA AACCCCAAGG ATAAAAAAGA AAAGGAGCTG 
GATGGACTAG GGAAAAGACG AAAGACGAGC CAGGCTGGAC AATCGGAGGA CGAGGAGCTG 
CAGAGGGCTA ATCGAGACGA CAGGCCATCT ATAGCCACAG CTCTGGCAGA ACGTAAGATC 
GACTCCGCTG CAGCAGCCAA CAATAACAAC AAAGTGAAGT CGATGGCCAC GCAGCTGCTG 
GCCAAGTTTG AGGAGAACGC GCCGACACAG TCCACTGGGC TCAAACGACA GGGAGACTCT 
ATGCCCGATC TGGGCCTCAT ACTGACCCCT TCTCCAGCCG CTGCTCCTCT GAACGAGTCA 
GTTCGTCTGG CTCCAGTGCC TGCATGGAGG AAGAGGCGCA CACAGTTACA GGAGCAGATG 
AGTTTCCGCT ATAAAGAAAA ACTCAAGTGT CAAACTCTCC CCATCACACA GGAGCAGGCC 
AGCAGTGGGA CTGAGGTCTT GAGGAGCTGC CCTAAGAAAA CCATTCTTCT TTCTTCTTCC 
TCACCCTCAT CTTCCTCATT CTCTTCATCG TTTCCTCCAC ATGCACAGGG TTCCTTTCGA 
AAGGAGTTCC CTCAGAACAT CGGTGGCAGC GATGTGTGTT TCTTCTGCCG TAAACGGGTC 
TATGTGATGG AGCGTCTCAG CGCTGAAGGA AAGTTTTTCC ATCGCAGCTG CTTCAAGTGC 
GACTACTGCG GCACAACCCT CCGCCTGTCC TCATATGCCT TCGATGTTGA AGACGGGAAG 
TTTTATTGTA AACCACACTA CTGCTACCGA CTCTCTGGTG TGGCACAGAG AAAACGGCCT 
GCACCTGCTG CTGCTCCAGC AAACGCCAAG GAGCCCCAGG TGTTGGCGGT ATCCCCCAAC 
ACAGTGGACG CCCCAGGTCA AGCCATAACC ACTCAGACCC CTGCGGAGCG CCGGCCCTCA 
GAAACTGAGG TAAATGGTGT CACGGAGCCC AGCGTGGCCA AACGTCTGAA AGGGACTCCG 
GAAAGGATCG AGCTGGAGAA CTATCGTTTG TCAATGATGA GGGAGGAGGA GCTGGAGGAG 
GTGCCGGAGG AGACATTGGC GGAGCACAAC CTCAGCAGTG TGCTGGATAA AGCCACCGAC 
ATCGAGGAGG GATCCAGCAG CTCTGAGTCT GACATGGAGG AAGAGGATGA AGATGCAGAG 
GCGGCCGGTC CGTCTGATCT GGGTGGAGTT CCCTGGAAGG AGGCAGTAGA GCTCCATGCT 
AAGTTAAAGG GAGAAAGCGA CCCAGGAGCT GACGATGATG GCCTTCATGA CGGTGATGGA 
GAGATGGACG AGGATGAAGA AGAGGAGGAA GATGAGGAAG ATGAAGATGA GGAGGAAGAA 
GAGGAATCGA GCGAAGAGCC CTGTGAAGAG GACGATGACC CTGAAGCTGA AGCAGGCAGT 
CCTGACTTTG AGCCAGGCAC TGAAATAGAT CAAGAGGACA TTCCCTCAGA TGCAGAGGCT 
GAAGCTCGTT CACGGTGCAT AGATGAAGTG GTGACACTTC CAGTGGATAA TGATAAAACA 
GAAAGCCAGG GACAGGTTTT CAACACTGCA GAGAAAACGT CTGCAAATCC AAGGGAGTTG 
ATTGTGGACG TTGTGCTGTC TCCAATCCAA AAACCTGCTT TACCCATTGA GGAGGTGCAT 
GAAGTTTCTC CTGTAGTACT AGTTAAATCT CCTGGTGCAC GTTTCTTCCC TGAGCCCTAT 
CTGCCTGATA AAGTCAAGCA GAACATTCCC CCGCCTCAGA GTCCTGATGT TAAAACCCCT 
CATTCTCCTG TAGCTCAAAT CATTGCGCTG TCGCCCATCT GTTCCCAGCC TGTTCCACAA 
CCAGGCACAG CATCTCCCAA ATCTCCAGTC CAACCACAGC CTTGTGCCTG TTCTCCAACA 
GGCAATCCTT TGTCTCCGAT TTGTACCCAA TCGCAACCCT GCAATGAGCC ACCTTCACCC 
CTATCCACAA GCTCCCCCGT CAGGACTCAA CCAGTCCCAG CAGTAACTTC TACTCCTTTA 
GCTAAACCTG CTTCCGAGAA TAGGACTAAT GAACATTTGA AAGATTCAAC TCCTGAGCTT 
AAGAAAACAG ACCTCATTGA GGAGTTCTGG TTGAAGAGTG CAGAGATTAG AAAAAGTTTA 
GGGCTCACTC CCCTGGAACG AAGCAAAACT GCGGTGGAGA AGAGCATTGT GAAAACTCCA 
ACACCCGAAT CATCATCTCC TAAGTCTTAT ACCCCAGAGG ACTTGTCTGA GGAACAGAAG 
CCTACATTTA CGGGCCGTTC TATCATACGC AGGATCAATA TTACCTTGGA GGGTCAGGTT 
ATATCTCCGG TGGAACCTAA AAGTAATGGT TCAGAAAAGA AGGACTTAAG CAGCAGTTCA 
GGGTTGGGTC TAAACGGAAG TGTGACCACC AGTCAGACAG CTGCTAGCGA CAGCTACAAC 
AACTCTGATT CTACAATGCT TACCCCTCCT TCCAGTCCAC CACCTCCTCC ACCCAGGGAG 
GAACCTGCTT GTCTCCAAAA CAAGAAGTCT CAGGTTTCCT GGGACAACCT CCTTGAAGGC 
ACAGAAGAAC CCAAATCTGA GACCATGCCC ATTAAGCCCA GGACTCCAGT TAGTCCCCCT 
CAACCAAAAC AAAAACCAGT TACAGCTCCA GTGCCAACCC CTAGAACCAA TCCACCTGTT 
GTGATGAGGG TTAAGGAGCC CAATAAACCA CGTCGAGAAG AAGTGAGAAA GTCTTTTGTT 
GAGTGTGTAG ACGAGATACC ATTTGCTGAT GATGTTGAAG ACACATACGA TGACCGCACT 
CCAGATGCAA GTGGTCTTGA AAAGTTTTAC ACACCGCCGA CTAGCAAGGT CAACCGGGAT 
AAGCCGCCTC TCCACTTGGC ACTGGCTATG GAAAATGGCA AGCCCAATAT CCCTGGAGTT 
TCCCGAACAG CAAAGGGTTC CCAGCATTTT TCTCCTGAAG CCAAGGAGAT CGCAGAGGAG 
CGAATGAGGG CACGGGAAAA GTCTGTGAAG AGCCAAGCTC TTAAAGATGC AATGGCCAAG 
CAGCTTACAA AGATGAAAGA TTCTGAGGTT GCCAAGGGGG CTGTAGCAAA AGTAGCCTGG 
GATATTCCTG AAACAAAAGG AAAAAGCAAA AAGCAATCTA AAGCCCAGAA GGACTCTGCA 
GTGAAGGCCT TGGAGTCCAA AAAGCAAGCA GACACACTTC CAGACCGTTT CTTCTCTACA 
CCATCTAGTA AGGCCTTAGA CAGCTCTGTC ACATCTTCAG AGAGCTCCAC AGGGGGCAAA 
AGCAAGAAAC GCAGCTCACT GTTTTCTCCA CGCAAAAACA AAAAAGAAAA GAAAGCCAAA 
AATGAAAGAC TGTCCAGCAC TGAAGAAACG CCGCCCAAGC ACAAGTCCCT TTGGAAAGCA 
GTGTTCTCCG GCTACAAGAA GGACAAAAAG AAGAAAGATG ACAAGTCATG CCCAAGTACA 
CCCTCAAGCT CTACAACTGG AGACTCGGGG AAGAAGAAAG ATTCACCCCT GGACAGGTCC 
TCAGATTTAC GTCTGAGGAG AAATCTAAGC TTTTCTGAGG ACTCTGATCT CTCCTGTGAC 
GATGTTCTGG AAAGATCCTC TCAGAAGTCC AAGGGTGATC GTCATGTGGA CCTGTTTGAC 
TTGGTGTCAG ATTTGAAAAA AGAAGCCATT AAAGAGGAGA AAGCAAAAGA GAAGGCTAAG 
GATAGGGCAA AGGAAAGGGG AAGAGTTACT GAGAAGGGCA AGGAAAGGTC AAGAGAAAGG 
GAGAATGAAA AAAGAGTCAA AGAAAAGATG GTGAAAATGA AAGATGTGGA ACGGGAGAAA 
GGAAAAGAGG AAGAGTCTGT TTATGTACCT CATGCATTGG CGTTCAAGAG ATCATATGCC 
ACAAAGAAGA CATACACGGA GGAGGAACTG AACGCCAAGC TGACAAGAAG AGTACAGAAA 
GCTGCACGGA GGCAGGCCAA GCAGGAGGAG CTCAAGAGAC TTCACAGAGC TCAGATGATT 
CAGAGGCAGC TGGAGCAGGT GGAAGAGAAA CAGAGGCAGT TAGAGGAGAG AGGCGTCGCG 
GTGGAAAAGG CACTGCGAGG CGAAGCAGTT GAGCCCTATG TCGGGACCCC TCGCCGAAGA 
GCCCTGTACC TCTGCCCCTG CTGTGCCCCT GAAGGAATGG GTAAAAAGGA CGATCCGAAG 
CTGATGCAGG AATGGTTTAA ACTGGTCCAG GAGAAGAATG CCTTGGTACG ATATGAATCC 
GAGCTGATGA TATTTGCTCG GGAGCTAGAG TTGGAGGACA GACAGAGTCG ACTGCAACAG 
GAACTACGGG AACGCATGGC AATAGATGAT CATCTGAAGA CAGAGGAGGA GCTGGCAGAG 
GAGAAGCAGA TTCTGAATGA GATGTTGGAG GTGGTGGAGC AGCGAGACTC TCTGGTGGCC 
CTGCTGGAGG AACAGAGACT CAGGGAGAAG GAAGAAGATA AAGACCTGGA GGCGGTGATG 
CTCTCCAAAG GATTCAACCT CAACTGGGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3A ( XM_021473237.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021328912.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X4, mRNA.

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CloneID	ODa145981
Clone ID Related Accession (Same CDS sequence)	XM_021473237.1 , XM_021467760.1
Accession Version	XM_021473237.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3a isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395007.1) and transcript sequence (GFIL01026059.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1201 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAAATT 
CTGCTTGAGA AGCAATTGCA AGAGTCCATC GTGCGCTCCA GTAAGCTGCT GAACTGGTGT 
CAGAGGCAGA CGGAAGGCTA CCGAGGTGTC AGCGTGTCTG ATCTCACCAC GTCCTGGAAG 
AGTGGTTTGG CCCTGTGCGC GCTCATCCAC AGATACAGAC CAGACCTCAT TGACTTTGAA 
TCACTGGACG AGAAAGACGT GGAGAAGAAC AACCAGCTGG CTTTCGACGT GGCTGAAAGA 
GAGTTTGGGA TTTCTCCCAT CATGACCGGG AAGGAGATGT CTGTTGTGGT GGAGCCAGAT 
AAGCTCTCCA TGGTGATGTA CCTCAGTCAG TTCTACGAGA TGTTCAAGGA CACGGTGCCA 
CCTGGTGAAA ACCAGAATCT GAGCCCTGAG GAGAAGGCCG CACTGATCGC CAGCACTAAA 
TCACCCATCT CATTCCTTAG TAAGCTAGGT CAAAGCATCG CAATCTCCCG CAAAAGAAAC 
CCCAAGGATA AAAAAGAAAA GGAGCTGGAT GGACTAGGGA AAAGACGAAA GACGAGCCAG 
GCTGGACAAT CGGAGGACGA GGAGCTGCAG AGGGCTAATC GAGACGACAG GCCATCTATA 
GCCACAGCTC TGGCAGAACG TAAGATCGAC TCCGCTGCAG CAGCCAACAA TAACAACAAA 
GTGAAGTCGA TGGCCACGCA GCTGCTGGCC AAGTTTGAGG AGAACGCGCC GACACAGTCC 
ACTGGGCTCA AACGACAGGG AGACTCTATG CCCGATCTGG GCCTCATACT GACCCCTTCT 
CCAGCCGCTG CTCCTCTGAA CGAGTCAGTT CGTCTGGCTC CAGTGCCTGC ATGGAGGAAG 
AGGCGCACAC AGTTACAGGA GCAGATGAGT TTCCGCTATA AAGAAAAACT CAAGTGTCAA 
ACTCTCCCCA TCACACAGGA GCAGGCCAGC AGTGGGACTG AGGTCTTGAG GAGCTGCCCT 
AAGAAAACCA TTCTTCTTTC TTCTTCCTCA CCCTCATCTT CCTCATTCTC TTCATCGTTT 
CCTCCACATG CACAGTCAGT GATACACAAG TGTAATGGTG TTGGTGATGG AGAGAACAGA 
TTCCAGGACC TTTCAGTCTG TTACTGGGAA GATAATCTGC CTAATGTGGA CAAACCAGAA 
CCCATACATA TTCGTAGCAT TCAAGAGCGA GCGGAGACTC TAGTGGCTAG ATTTAAAGGC 
AAGGCTGACA GGAAGGACAA GCCACAGGTT ATGAAAAAGC CATCACGGTT CTTTATTGAG 
CAGTGGTTTT TGACCCACGG CCTCAGTCCT GATGAGCCCG TCTTCTCCTC TGACCCTCAG 
CATCATCAGC AAAAAGGGGT TTTGTGTCCT GACGATCACG TGCCTTTACA CATCCCCAGC 
ATTCAGGAGA GAGCCGAGAG ACTGGCCTCT CACTTTAAAG ACAAACCGGC GAAGCCAAAG 
TCTAAGAAAA AGTCGTCACT GTTGTTGACA GAGCAGTGGT CCAGGGCTCG TGATGTGAGC 
TCTGCTAGTC CTCTGCATTC CCCCAGCTCT TTCCGGCAGC ACTGTGTAAA GATGTACACA 
GGGGGTGTGA GCTCATTGGC TGAGCAAATA GCCAATCAGC TTCAGGCTAA GGACGACCCC 
AGACCCATGC TTGAGAAAAG GGATTTGGGT TCCTTTCGAA AGGAGTTCCC TCAGAACATC 
GGTGGCAGCG ATGTGTGTTT CTTCTGCCGT AAACGGGTCT ATGTGATGGA GCGTCTCAGC 
GCTGAAGGAA AGTTTTTCCA TCGCAGCTGC TTCAAGTGCG ACTACTGCGG CACAACCCTC 
CGCCTGTCCT CATATGCCTT CGATGTTGAA GACGGGAAGT TTTATTGTAA ACCACACTAC 
TGCTACCGAC TCTCTGGTGT GGCACAGAGA AAACGGCCTG CACCTGCTGC TGCTCCAGCA 
AACGCCAAGG AGCCCCAGGT GTTGGCGGTA TCCCCCAACA CAGTGGACGC CCCAGGTCAA 
GCCATAACCA CTCAGACCCC TGCGGAGCGC CGGCCCTCAG AAACTGAGGT AAATGGTGTC 
ACGGAGCCCA GCGTGGCCAA ACGTCTGAAA GGGACTCCGG AAAGGATCGA GCTGGAGAAC 
TATCGTTTGT CAATGATGAG GGAGGAGGAG CTGGAGGAGG TGCCGGAGGA GACATTGGCG 
GAGCACAACC TCAGCAGTGT GCTGGATAAA GCCACCGACA TCGAGGAGGG ATCCAGCAGC 
TCTGAGTCTG ACATGGAGGA AGAGGATGAA GATGCAGAGG CGGCCGGTCC GTCTGATCTG 
GGTGGAGTTC CCTGGAAGGA GGCAGTAGAG CTCCATGCTA AGTTAAAGGG AGAAAGCGAC 
CCAGGAGCTG ACGATGATGG CCTTCATGAC GGTGATGGAG AGATGGACGA GGATGAAGAA 
GAGGAGGAAG ATGAGGAAGA TGAAGATGAG GAGGAAGAAG AGGAATCGAG CGAAGCGGGA 
GACTATTTCC CCTGGGAGAG GGAGCTTCAG TCAGGCCTTT GGCTGGAAAA TCTGGCAAAT 
GAAGAGGATG TCAGTGTTTT TAAAGCTAGG AACCTGCAGA TCCAGCAAGC CATTCATCCA 
GTAGACCCTC TGGACATCCA GATGGAGAGC CATCAGGCTG AGGGGGTGTT GGAGATGCCC 
TCGGTGTCAC CCTTAGGCTC ACAGCCCTCG CTCAACACCC AGCTCACACA GCCCACCTCC 
TCCACAGCCC CCTCCCACAA ATCCGTACGT CACGAGGCTG TCAGAGCCTG GCTGGACTCG 
ATGTCTGGAG AGCCCTGTGA AGAGGACGAT GACCCTGAAG CTGAAGCAGG CAGTCCTGAC 
TTTGAGCCAG GCACTGAAAT AGATCAAGAG GACATTCCCT CAGATGCAGA GGCTGAAGCT 
CGTTCACGGT GCATAGATGA AGTGGTGACA CTTCCAGTGG ATAATGATAA AACAGAAAGC 
CAGGGACAGG TTTTCAACAC TGCAGAGAAA ACGTCTGCAA ATCCAAGGGA GTTGATTGTG 
GACGTTGTGC TGTCTCCAAT CCAAAAACCT GCTTTACCCA TTGAGGAGGT GCATGAAGTT 
TCTCCTGTAG TACTAGTTAA ATCTCCTGGT GCACGTTTCT TCCCTGAGCC CTATCTGCCT 
GATAAAGTCA AGCAGAACAT TCCCCCGCCT CAGAGTCCTG ATGTTAAAAC CCCTCATTCT 
CCTGTAGCTC AAATCATTGC GCTGTCGCCC ATCTGTTCCC AGCCTGTTCC ACAACCAGGC 
ACAGCATCTC CCAAATCTCC AGTCCAACCA CAGCCTTGTG CCTGTTCTCC AACAGGCAAT 
CCTTTGTCTC CGATTTGTAC CCAATCGCAA CCCTGCAATG AGCCACCTTC ACCCCTATCC 
ACAAGCTCCC CCGTCAGGAC TCAACCAGTC CCAGCAGTAA CTTCTACTCC TTTAGCTAAA 
CCTGCTTCCG AGAATAGGAC TAATGAACAT TTGAAAGATT CAACTCCTGA GCTTAAGAAA 
ACAGACCTCA TTGAGGAGTT CTGGTTGAAG AGTGCAGAGA TTAGAAAAAG TTTAGGGCTC 
ACTCCCCTGG AACGAAGCAA AACTGCGGTG GAGAAGAGCA TTGTGAAAAC TCCAACACCC 
GAATCATCAT CTCCTAAGTC TTATACCCCA GAGGACTTGT CTGAGGAACA GAAGCCTACA 
TTTACGGGCC GTTCTATCAT ACGCAGGATC AATATTACCT TGGAGGGTCA GGTTATATCT 
CCGGTGGAAC CTAAAAGTAA TGGTTCAGAA AAGAAGGACT TAAGCAGCAG TTCAGGGTTG 
GGTCTAAACG GAAGTGTGAC CACCAGTCAG ACAGCTGCTA GCGACAGCTA CAACAACTCT 
GATTCTACAA TGCTTACCCC TCCTTCCAGT CCACCACCTC CTCCACCCAG GGAGGAACCT 
GCTTGTCTCC AAAACAAGAA GTCTCAGGTT TCCTGGGACA ACCTCCTTGA AGGCACAGAA 
GAACCCAAAT CTGAGACCAT GCCCATTAAG CCCAGGACTC CAGTTAGTCC CCCTCAACCA 
AAACAAAAAC CAGTTACAGC TCCAGTGCCA ACCCCTAGAA CCAATCCACC TGTTGTGATG 
AGGGTTAAGG AGCCCAATAA ACCACGTCGA GAAGAAGTGA GAAAGTCTTT TGTTGAGTGT 
GTAGACGAGA TACCATTTGC TGATGATGTT GAAGACACAT ACGATGACCG CACTCCAGAT 
GCAAGTGGTC TTGAAAAGTT TTACACACCG CCGACTAGCA AGGTCAACCG GGATAAGCCG 
CCTCTCCACT TGGCACTGGC TATGGAAAAT GGCAAGCCCA ATATCCCTGG AGTTTCCCGA 
ACAGCAAAGG GTTCCCAGCA TTTTTCTCCT GAAGCCAAGG AGATCGCAGA GGAGCGAATG 
AGGGCACGGG AAAAGTCTGT GAAGAGCCAA GCTCTTAAAG ATGCAATGGC CAAGCAGCTT 
ACAAAGATGA AAGATTCTGA GGTTGCCAAG GGGGCTGTAG CAAAAGTAGC CTGGGATATT 
CCTGAAACAA AAGGAAAAAG CAAAAAGCAA TCTAAAGCCC AGAAGGACTC TGCAGTGAAG 
GCCTTGGAGT CCAAAAAGCA AGCAGACACA CTTCCAGACC GTTTCTTCTC TACACCATCT 
AGTAAGGCCT TAGACAGCTC TGTCACATCT TCAGAGAGCT CCACAGGGGG CAAAAGCAAG 
AAACGCAGCT CACTGTTTTC TCCACGCAAA AACAAAAAAG AAAAGAAAGC CAAAAATGAA 
AGACTGTCCA GCACTGAAGA AACGCCGCCC AAGCACAAGT CCCTTTGGAA AGCAGTGTTC 
TCCGGCTACA AGAAGGACAA AAAGAAGAAA GATGACAAGT CATGCCCAAG TACACCCTCA 
AGCTCTACAA CTGGAGACTC GGGGAAGAAG AAAGATTCAC CCCTGGACAG GTCCTCAGAT 
TTACGTCTGA GGAGAAATCT AAGCTTTTCT GAGGACTCTG ATCTCTCCTG TGACGATGTT 
CTGGAAAGAT CCTCTCAGAA GTCCAAGGGT GATCGTCATG TGGACCTGTT TGACTTGGTG 
TCAGATTTGA AAAAAGAAGC CATTAAAGAG GAGAAAGCAA AAGAGAAGGC TAAGGATAGG 
GCAAAGGAAA GGGGAAGAGT TACTGAGAAG GGCAAGGAAA GGTCAAGAGA AAGGGAGAAT 
GAAAAAAGAG TCAAAGAAAA GATGGTGAAA ATGAAAGATG TGGAACGGGA GAAAGGAAAA 
GAGGAAGAGT CTGTTTATGT ACCTCATGCA TTGGCGTTCA AGAGATCATA TGCCACAAAG 
AAGACATACA CGGAGGAGGA ACTGAACGCC AAGCTGACAA GAAGAGTACA GAAAGCTGCA 
CGGAGGCAGG CCAAGCAGGA GGAGCTCAAG AGACTTCACA GAGCTCAGAT GATTCAGAGG 
CAGCTGGAGC AGGTGGAAGA GAAACAGAGG CAGTTAGAGG AGAGAGGCGT CGCGGTGGAA 
AAGGCACTGC GAGGCGAAGC AGGGTTGTAT AAAGGTTCTA GCGTTAGTCC AAAACAGCGT 
AAGAGACGCT CAGACTATTG GGGAGAGTCT AATTACAGTG AGATCCTGGA TTTGCATCTG 
GGCGTTGAGC CCTATGTCGG GACCCCTCGC CGAAGAGCCC TGTACCTCTG CCCCTGCTGT 
GCCCCTGAAG GAATGGGTAA AAAGGACGAT CCGAAGCTGA TGCAGGAATG GTTTAAACTG 
GTCCAGGAGA AGAATGCCTT GGTACGATAT GAATCCGAGC TGATGATATT TGCTCGGGAG 
CTAGAGTTGG AGGACAGACA GAGTCGACTG CAACAGGAAC TACGGGAACG CATGGCAATA 
GATGATCATC TGAAGACAGA GGAGGAGCTG GCAGAGGAGA AGCAGATTCT GAATGAGATG 
TTGGAGGTGG TGGAGCAGCG AGACTCTCTG GTGGCCCTGC TGGAGGAACA GAGACTCAGG 
GAGAAGGAAG AAGATAAAGA CCTGGAGGCG GTGATGCTCT CCAAAGGATT CAACCTCAAC 
TGGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328912.1
CDS	349..7917
Translation	MGDGGVNAVGEGVNQSHMLFDRFVQATTCKGTLKAFQELCDFLELKPNEYRVFYHKLKSKLNYWKAKALWAKLDKRASHKEYKKGRACANTKCLIIGAGPCGLRTAIELGFLGAKVVLLEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGIEIHVNVEFKGLIEPPEDQENERIGWRAEVHPRTHPVNELEFDVIIGADGRRNTLSGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILHDYADTEMLLSRANVDQKALLSYAREAADFSTNHQLPKLDFAINHYGQPDVAMFDFTCMYASENAALVRQRNGHKLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWAHGSSPLEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNISLHQVRPNQVRHLLDTGETRDLRVDLENVVNSSTPKLTRNEILLEKQLQESIVRSSKLLNWCQRQTEGYRGVSVSDLTTSWKSGLALCALIHRYRPDLIDFESLDEKDVEKNNQLAFDVAEREFGISPIMTGKEMSVVVEPDKLSMVMYLSQFYEMFKDTVPPGENQNLSPEEKAALIASTKSPISFLSKLGQSIAISRKRNPKDKKEKELDGLGKRRKTSQAGQSEDEELQRANRDDRPSIATALAERKIDSAAAANNNNKVKSMATQLLAKFEENAPTQSTGLKRQGDSMPDLGLILTPSPAAAPLNESVRLAPVPAWRKRRTQLQEQMSFRYKEKLKCQTLPITQEQASSGTEVLRSCPKKTILLSSSSPSSSSFSSSFPPHAQSVIHKCNGVGDGENRFQDLSVCYWEDNLPNVDKPEPIHIRSIQERAETLVARFKGKADRKDKPQVMKKPSRFFIEQWFLTHGLSPDEPVFSSDPQHHQQKGVLCPDDHVPLHIPSIQERAERLASHFKDKPAKPKSKKKSSLLLTEQWSRARDVSSASPLHSPSSFRQHCVKMYTGGVSSLAEQIANQLQAKDDPRPMLEKRDLGSFRKEFPQNIGGSDVCFFCRKRVYVMERLSAEGKFFHRSCFKCDYCGTTLRLSSYAFDVEDGKFYCKPHYCYRLSGVAQRKRPAPAAAPANAKEPQVLAVSPNTVDAPGQAITTQTPAERRPSETEVNGVTEPSVAKRLKGTPERIELENYRLSMMREEELEEVPEETLAEHNLSSVLDKATDIEEGSSSSESDMEEEDEDAEAAGPSDLGGVPWKEAVELHAKLKGESDPGADDDGLHDGDGEMDEDEEEEEDEEDEDEEEEEESSEAGDYFPWERELQSGLWLENLANEEDVSVFKARNLQIQQAIHPVDPLDIQMESHQAEGVLEMPSVSPLGSQPSLNTQLTQPTSSTAPSHKSVRHEAVRAWLDSMSGEPCEEDDDPEAEAGSPDFEPGTEIDQEDIPSDAEAEARSRCIDEVVTLPVDNDKTESQGQVFNTAEKTSANPRELIVDVVLSPIQKPALPIEEVHEVSPVVLVKSPGARFFPEPYLPDKVKQNIPPPQSPDVKTPHSPVAQIIALSPICSQPVPQPGTASPKSPVQPQPCACSPTGNPLSPICTQSQPCNEPPSPLSTSSPVRTQPVPAVTSTPLAKPASENRTNEHLKDSTPELKKTDLIEEFWLKSAEIRKSLGLTPLERSKTAVEKSIVKTPTPESSSPKSYTPEDLSEEQKPTFTGRSIIRRINITLEGQVISPVEPKSNGSEKKDLSSSSGLGLNGSVTTSQTAASDSYNNSDSTMLTPPSSPPPPPPREEPACLQNKKSQVSWDNLLEGTEEPKSETMPIKPRTPVSPPQPKQKPVTAPVPTPRTNPPVVMRVKEPNKPRREEVRKSFVECVDEIPFADDVEDTYDDRTPDASGLEKFYTPPTSKVNRDKPPLHLALAMENGKPNIPGVSRTAKGSQHFSPEAKEIAEERMRAREKSVKSQALKDAMAKQLTKMKDSEVAKGAVAKVAWDIPETKGKSKKQSKAQKDSAVKALESKKQADTLPDRFFSTPSSKALDSSVTSSESSTGGKSKKRSSLFSPRKNKKEKKAKNERLSSTEETPPKHKSLWKAVFSGYKKDKKKKDDKSCPSTPSSSTTGDSGKKKDSPLDRSSDLRLRRNLSFSEDSDLSCDDVLERSSQKSKGDRHVDLFDLVSDLKKEAIKEEKAKEKAKDRAKERGRVTEKGKERSRERENEKRVKEKMVKMKDVEREKGKEEESVYVPHALAFKRSYATKKTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQMIQRQLEQVEEKQRQLEERGVAVEKALRGEAGLYKGSSVSPKQRKRRSDYWGESNYSEILDLHLGVEPYVGTPRRRALYLCPCCAPEGMGKKDDPKLMQEWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAIDDHLKTEEELAEEKQILNEMLEVVEQRDSLVALLEEQRLREKEEDKDLEAVMLSKGFNLNWA

Target ORF information:

RefSeq Version	XM_021473237.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473237.1

ORF Insert Sequence:

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAAATT 
CTGCTTGAGA AGCAATTGCA AGAGTCCATC GTGCGCTCCA GTAAGCTGCT GAACTGGTGT 
CAGAGGCAGA CGGAAGGCTA CCGAGGTGTC AGCGTGTCTG ATCTCACCAC GTCCTGGAAG 
AGTGGTTTGG CCCTGTGCGC GCTCATCCAC AGATACAGAC CAGACCTCAT TGACTTTGAA 
TCACTGGACG AGAAAGACGT GGAGAAGAAC AACCAGCTGG CTTTCGACGT GGCTGAAAGA 
GAGTTTGGGA TTTCTCCCAT CATGACCGGG AAGGAGATGT CTGTTGTGGT GGAGCCAGAT 
AAGCTCTCCA TGGTGATGTA CCTCAGTCAG TTCTACGAGA TGTTCAAGGA CACGGTGCCA 
CCTGGTGAAA ACCAGAATCT GAGCCCTGAG GAGAAGGCCG CACTGATCGC CAGCACTAAA 
TCACCCATCT CATTCCTTAG TAAGCTAGGT CAAAGCATCG CAATCTCCCG CAAAAGAAAC 
CCCAAGGATA AAAAAGAAAA GGAGCTGGAT GGACTAGGGA AAAGACGAAA GACGAGCCAG 
GCTGGACAAT CGGAGGACGA GGAGCTGCAG AGGGCTAATC GAGACGACAG GCCATCTATA 
GCCACAGCTC TGGCAGAACG TAAGATCGAC TCCGCTGCAG CAGCCAACAA TAACAACAAA 
GTGAAGTCGA TGGCCACGCA GCTGCTGGCC AAGTTTGAGG AGAACGCGCC GACACAGTCC 
ACTGGGCTCA AACGACAGGG AGACTCTATG CCCGATCTGG GCCTCATACT GACCCCTTCT 
CCAGCCGCTG CTCCTCTGAA CGAGTCAGTT CGTCTGGCTC CAGTGCCTGC ATGGAGGAAG 
AGGCGCACAC AGTTACAGGA GCAGATGAGT TTCCGCTATA AAGAAAAACT CAAGTGTCAA 
ACTCTCCCCA TCACACAGGA GCAGGCCAGC AGTGGGACTG AGGTCTTGAG GAGCTGCCCT 
AAGAAAACCA TTCTTCTTTC TTCTTCCTCA CCCTCATCTT CCTCATTCTC TTCATCGTTT 
CCTCCACATG CACAGTCAGT GATACACAAG TGTAATGGTG TTGGTGATGG AGAGAACAGA 
TTCCAGGACC TTTCAGTCTG TTACTGGGAA GATAATCTGC CTAATGTGGA CAAACCAGAA 
CCCATACATA TTCGTAGCAT TCAAGAGCGA GCGGAGACTC TAGTGGCTAG ATTTAAAGGC 
AAGGCTGACA GGAAGGACAA GCCACAGGTT ATGAAAAAGC CATCACGGTT CTTTATTGAG 
CAGTGGTTTT TGACCCACGG CCTCAGTCCT GATGAGCCCG TCTTCTCCTC TGACCCTCAG 
CATCATCAGC AAAAAGGGGT TTTGTGTCCT GACGATCACG TGCCTTTACA CATCCCCAGC 
ATTCAGGAGA GAGCCGAGAG ACTGGCCTCT CACTTTAAAG ACAAACCGGC GAAGCCAAAG 
TCTAAGAAAA AGTCGTCACT GTTGTTGACA GAGCAGTGGT CCAGGGCTCG TGATGTGAGC 
TCTGCTAGTC CTCTGCATTC CCCCAGCTCT TTCCGGCAGC ACTGTGTAAA GATGTACACA 
GGGGGTGTGA GCTCATTGGC TGAGCAAATA GCCAATCAGC TTCAGGCTAA GGACGACCCC 
AGACCCATGC TTGAGAAAAG GGATTTGGGT TCCTTTCGAA AGGAGTTCCC TCAGAACATC 
GGTGGCAGCG ATGTGTGTTT CTTCTGCCGT AAACGGGTCT ATGTGATGGA GCGTCTCAGC 
GCTGAAGGAA AGTTTTTCCA TCGCAGCTGC TTCAAGTGCG ACTACTGCGG CACAACCCTC 
CGCCTGTCCT CATATGCCTT CGATGTTGAA GACGGGAAGT TTTATTGTAA ACCACACTAC 
TGCTACCGAC TCTCTGGTGT GGCACAGAGA AAACGGCCTG CACCTGCTGC TGCTCCAGCA 
AACGCCAAGG AGCCCCAGGT GTTGGCGGTA TCCCCCAACA CAGTGGACGC CCCAGGTCAA 
GCCATAACCA CTCAGACCCC TGCGGAGCGC CGGCCCTCAG AAACTGAGGT AAATGGTGTC 
ACGGAGCCCA GCGTGGCCAA ACGTCTGAAA GGGACTCCGG AAAGGATCGA GCTGGAGAAC 
TATCGTTTGT CAATGATGAG GGAGGAGGAG CTGGAGGAGG TGCCGGAGGA GACATTGGCG 
GAGCACAACC TCAGCAGTGT GCTGGATAAA GCCACCGACA TCGAGGAGGG ATCCAGCAGC 
TCTGAGTCTG ACATGGAGGA AGAGGATGAA GATGCAGAGG CGGCCGGTCC GTCTGATCTG 
GGTGGAGTTC CCTGGAAGGA GGCAGTAGAG CTCCATGCTA AGTTAAAGGG AGAAAGCGAC 
CCAGGAGCTG ACGATGATGG CCTTCATGAC GGTGATGGAG AGATGGACGA GGATGAAGAA 
GAGGAGGAAG ATGAGGAAGA TGAAGATGAG GAGGAAGAAG AGGAATCGAG CGAAGCGGGA 
GACTATTTCC CCTGGGAGAG GGAGCTTCAG TCAGGCCTTT GGCTGGAAAA TCTGGCAAAT 
GAAGAGGATG TCAGTGTTTT TAAAGCTAGG AACCTGCAGA TCCAGCAAGC CATTCATCCA 
GTAGACCCTC TGGACATCCA GATGGAGAGC CATCAGGCTG AGGGGGTGTT GGAGATGCCC 
TCGGTGTCAC CCTTAGGCTC ACAGCCCTCG CTCAACACCC AGCTCACACA GCCCACCTCC 
TCCACAGCCC CCTCCCACAA ATCCGTACGT CACGAGGCTG TCAGAGCCTG GCTGGACTCG 
ATGTCTGGAG AGCCCTGTGA AGAGGACGAT GACCCTGAAG CTGAAGCAGG CAGTCCTGAC 
TTTGAGCCAG GCACTGAAAT AGATCAAGAG GACATTCCCT CAGATGCAGA GGCTGAAGCT 
CGTTCACGGT GCATAGATGA AGTGGTGACA CTTCCAGTGG ATAATGATAA AACAGAAAGC 
CAGGGACAGG TTTTCAACAC TGCAGAGAAA ACGTCTGCAA ATCCAAGGGA GTTGATTGTG 
GACGTTGTGC TGTCTCCAAT CCAAAAACCT GCTTTACCCA TTGAGGAGGT GCATGAAGTT 
TCTCCTGTAG TACTAGTTAA ATCTCCTGGT GCACGTTTCT TCCCTGAGCC CTATCTGCCT 
GATAAAGTCA AGCAGAACAT TCCCCCGCCT CAGAGTCCTG ATGTTAAAAC CCCTCATTCT 
CCTGTAGCTC AAATCATTGC GCTGTCGCCC ATCTGTTCCC AGCCTGTTCC ACAACCAGGC 
ACAGCATCTC CCAAATCTCC AGTCCAACCA CAGCCTTGTG CCTGTTCTCC AACAGGCAAT 
CCTTTGTCTC CGATTTGTAC CCAATCGCAA CCCTGCAATG AGCCACCTTC ACCCCTATCC 
ACAAGCTCCC CCGTCAGGAC TCAACCAGTC CCAGCAGTAA CTTCTACTCC TTTAGCTAAA 
CCTGCTTCCG AGAATAGGAC TAATGAACAT TTGAAAGATT CAACTCCTGA GCTTAAGAAA 
ACAGACCTCA TTGAGGAGTT CTGGTTGAAG AGTGCAGAGA TTAGAAAAAG TTTAGGGCTC 
ACTCCCCTGG AACGAAGCAA AACTGCGGTG GAGAAGAGCA TTGTGAAAAC TCCAACACCC 
GAATCATCAT CTCCTAAGTC TTATACCCCA GAGGACTTGT CTGAGGAACA GAAGCCTACA 
TTTACGGGCC GTTCTATCAT ACGCAGGATC AATATTACCT TGGAGGGTCA GGTTATATCT 
CCGGTGGAAC CTAAAAGTAA TGGTTCAGAA AAGAAGGACT TAAGCAGCAG TTCAGGGTTG 
GGTCTAAACG GAAGTGTGAC CACCAGTCAG ACAGCTGCTA GCGACAGCTA CAACAACTCT 
GATTCTACAA TGCTTACCCC TCCTTCCAGT CCACCACCTC CTCCACCCAG GGAGGAACCT 
GCTTGTCTCC AAAACAAGAA GTCTCAGGTT TCCTGGGACA ACCTCCTTGA AGGCACAGAA 
GAACCCAAAT CTGAGACCAT GCCCATTAAG CCCAGGACTC CAGTTAGTCC CCCTCAACCA 
AAACAAAAAC CAGTTACAGC TCCAGTGCCA ACCCCTAGAA CCAATCCACC TGTTGTGATG 
AGGGTTAAGG AGCCCAATAA ACCACGTCGA GAAGAAGTGA GAAAGTCTTT TGTTGAGTGT 
GTAGACGAGA TACCATTTGC TGATGATGTT GAAGACACAT ACGATGACCG CACTCCAGAT 
GCAAGTGGTC TTGAAAAGTT TTACACACCG CCGACTAGCA AGGTCAACCG GGATAAGCCG 
CCTCTCCACT TGGCACTGGC TATGGAAAAT GGCAAGCCCA ATATCCCTGG AGTTTCCCGA 
ACAGCAAAGG GTTCCCAGCA TTTTTCTCCT GAAGCCAAGG AGATCGCAGA GGAGCGAATG 
AGGGCACGGG AAAAGTCTGT GAAGAGCCAA GCTCTTAAAG ATGCAATGGC CAAGCAGCTT 
ACAAAGATGA AAGATTCTGA GGTTGCCAAG GGGGCTGTAG CAAAAGTAGC CTGGGATATT 
CCTGAAACAA AAGGAAAAAG CAAAAAGCAA TCTAAAGCCC AGAAGGACTC TGCAGTGAAG 
GCCTTGGAGT CCAAAAAGCA AGCAGACACA CTTCCAGACC GTTTCTTCTC TACACCATCT 
AGTAAGGCCT TAGACAGCTC TGTCACATCT TCAGAGAGCT CCACAGGGGG CAAAAGCAAG 
AAACGCAGCT CACTGTTTTC TCCACGCAAA AACAAAAAAG AAAAGAAAGC CAAAAATGAA 
AGACTGTCCA GCACTGAAGA AACGCCGCCC AAGCACAAGT CCCTTTGGAA AGCAGTGTTC 
TCCGGCTACA AGAAGGACAA AAAGAAGAAA GATGACAAGT CATGCCCAAG TACACCCTCA 
AGCTCTACAA CTGGAGACTC GGGGAAGAAG AAAGATTCAC CCCTGGACAG GTCCTCAGAT 
TTACGTCTGA GGAGAAATCT AAGCTTTTCT GAGGACTCTG ATCTCTCCTG TGACGATGTT 
CTGGAAAGAT CCTCTCAGAA GTCCAAGGGT GATCGTCATG TGGACCTGTT TGACTTGGTG 
TCAGATTTGA AAAAAGAAGC CATTAAAGAG GAGAAAGCAA AAGAGAAGGC TAAGGATAGG 
GCAAAGGAAA GGGGAAGAGT TACTGAGAAG GGCAAGGAAA GGTCAAGAGA AAGGGAGAAT 
GAAAAAAGAG TCAAAGAAAA GATGGTGAAA ATGAAAGATG TGGAACGGGA GAAAGGAAAA 
GAGGAAGAGT CTGTTTATGT ACCTCATGCA TTGGCGTTCA AGAGATCATA TGCCACAAAG 
AAGACATACA CGGAGGAGGA ACTGAACGCC AAGCTGACAA GAAGAGTACA GAAAGCTGCA 
CGGAGGCAGG CCAAGCAGGA GGAGCTCAAG AGACTTCACA GAGCTCAGAT GATTCAGAGG 
CAGCTGGAGC AGGTGGAAGA GAAACAGAGG CAGTTAGAGG AGAGAGGCGT CGCGGTGGAA 
AAGGCACTGC GAGGCGAAGC AGGGTTGTAT AAAGGTTCTA GCGTTAGTCC AAAACAGCGT 
AAGAGACGCT CAGACTATTG GGGAGAGTCT AATTACAGTG AGATCCTGGA TTTGCATCTG 
GGCGTTGAGC CCTATGTCGG GACCCCTCGC CGAAGAGCCC TGTACCTCTG CCCCTGCTGT 
GCCCCTGAAG GAATGGGTAA AAAGGACGAT CCGAAGCTGA TGCAGGAATG GTTTAAACTG 
GTCCAGGAGA AGAATGCCTT GGTACGATAT GAATCCGAGC TGATGATATT TGCTCGGGAG 
CTAGAGTTGG AGGACAGACA GAGTCGACTG CAACAGGAAC TACGGGAACG CATGGCAATA 
GATGATCATC TGAAGACAGA GGAGGAGCTG GCAGAGGAGA AGCAGATTCT GAATGAGATG 
TTGGAGGTGG TGGAGCAGCG AGACTCTCTG GTGGCCCTGC TGGAGGAACA GAGACTCAGG 
GAGAAGGAAG AAGATAAAGA CCTGGAGGCG GTGATGCTCT CCAAAGGATT CAACCTCAAC 
TGGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3A ( XM_021467760.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021323435.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X1, mRNA.

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CloneID	ODa145981
Clone ID Related Accession (Same CDS sequence)	XM_021473237.1 , XM_021467760.1
Accession Version	XM_021467760.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAAATT 
CTGCTTGAGA AGCAATTGCA AGAGTCCATC GTGCGCTCCA GTAAGCTGCT GAACTGGTGT 
CAGAGGCAGA CGGAAGGCTA CCGAGGTGTC AGCGTGTCTG ATCTCACCAC GTCCTGGAAG 
AGTGGTTTGG CCCTGTGCGC GCTCATCCAC AGATACAGAC CAGACCTCAT TGACTTTGAA 
TCACTGGACG AGAAAGACGT GGAGAAGAAC AACCAGCTGG CTTTCGACGT GGCTGAAAGA 
GAGTTTGGGA TTTCTCCCAT CATGACCGGG AAGGAGATGT CTGTTGTGGT GGAGCCAGAT 
AAGCTCTCCA TGGTGATGTA CCTCAGTCAG TTCTACGAGA TGTTCAAGGA CACGGTGCCA 
CCTGGTGAAA ACCAGAATCT GAGCCCTGAG GAGAAGGCCG CACTGATCGC CAGCACTAAA 
TCACCCATCT CATTCCTTAG TAAGCTAGGT CAAAGCATCG CAATCTCCCG CAAAAGAAAC 
CCCAAGGATA AAAAAGAAAA GGAGCTGGAT GGACTAGGGA AAAGACGAAA GACGAGCCAG 
GCTGGACAAT CGGAGGACGA GGAGCTGCAG AGGGCTAATC GAGACGACAG GCCATCTATA 
GCCACAGCTC TGGCAGAACG TAAGATCGAC TCCGCTGCAG CAGCCAACAA TAACAACAAA 
GTGAAGTCGA TGGCCACGCA GCTGCTGGCC AAGTTTGAGG AGAACGCGCC GACACAGTCC 
ACTGGGCTCA AACGACAGGG AGACTCTATG CCCGATCTGG GCCTCATACT GACCCCTTCT 
CCAGCCGCTG CTCCTCTGAA CGAGTCAGTT CGTCTGGCTC CAGTGCCTGC ATGGAGGAAG 
AGGCGCACAC AGTTACAGGA GCAGATGAGT TTCCGCTATA AAGAAAAACT CAAGTGTCAA 
ACTCTCCCCA TCACACAGGA GCAGGCCAGC AGTGGGACTG AGGTCTTGAG GAGCTGCCCT 
AAGAAAACCA TTCTTCTTTC TTCTTCCTCA CCCTCATCTT CCTCATTCTC TTCATCGTTT 
CCTCCACATG CACAGTCAGT GATACACAAG TGTAATGGTG TTGGTGATGG AGAGAACAGA 
TTCCAGGACC TTTCAGTCTG TTACTGGGAA GATAATCTGC CTAATGTGGA CAAACCAGAA 
CCCATACATA TTCGTAGCAT TCAAGAGCGA GCGGAGACTC TAGTGGCTAG ATTTAAAGGC 
AAGGCTGACA GGAAGGACAA GCCACAGGTT ATGAAAAAGC CATCACGGTT CTTTATTGAG 
CAGTGGTTTT TGACCCACGG CCTCAGTCCT GATGAGCCCG TCTTCTCCTC TGACCCTCAG 
CATCATCAGC AAAAAGGGGT TTTGTGTCCT GACGATCACG TGCCTTTACA CATCCCCAGC 
ATTCAGGAGA GAGCCGAGAG ACTGGCCTCT CACTTTAAAG ACAAACCGGC GAAGCCAAAG 
TCTAAGAAAA AGTCGTCACT GTTGTTGACA GAGCAGTGGT CCAGGGCTCG TGATGTGAGC 
TCTGCTAGTC CTCTGCATTC CCCCAGCTCT TTCCGGCAGC ACTGTGTAAA GATGTACACA 
GGGGGTGTGA GCTCATTGGC TGAGCAAATA GCCAATCAGC TTCAGGCTAA GGACGACCCC 
AGACCCATGC TTGAGAAAAG GGATTTGGGT TCCTTTCGAA AGGAGTTCCC TCAGAACATC 
GGTGGCAGCG ATGTGTGTTT CTTCTGCCGT AAACGGGTCT ATGTGATGGA GCGTCTCAGC 
GCTGAAGGAA AGTTTTTCCA TCGCAGCTGC TTCAAGTGCG ACTACTGCGG CACAACCCTC 
CGCCTGTCCT CATATGCCTT CGATGTTGAA GACGGGAAGT TTTATTGTAA ACCACACTAC 
TGCTACCGAC TCTCTGGTGT GGCACAGAGA AAACGGCCTG CACCTGCTGC TGCTCCAGCA 
AACGCCAAGG AGCCCCAGGT GTTGGCGGTA TCCCCCAACA CAGTGGACGC CCCAGGTCAA 
GCCATAACCA CTCAGACCCC TGCGGAGCGC CGGCCCTCAG AAACTGAGGT AAATGGTGTC 
ACGGAGCCCA GCGTGGCCAA ACGTCTGAAA GGGACTCCGG AAAGGATCGA GCTGGAGAAC 
TATCGTTTGT CAATGATGAG GGAGGAGGAG CTGGAGGAGG TGCCGGAGGA GACATTGGCG 
GAGCACAACC TCAGCAGTGT GCTGGATAAA GCCACCGACA TCGAGGAGGG ATCCAGCAGC 
TCTGAGTCTG ACATGGAGGA AGAGGATGAA GATGCAGAGG CGGCCGGTCC GTCTGATCTG 
GGTGGAGTTC CCTGGAAGGA GGCAGTAGAG CTCCATGCTA AGTTAAAGGG AGAAAGCGAC 
CCAGGAGCTG ACGATGATGG CCTTCATGAC GGTGATGGAG AGATGGACGA GGATGAAGAA 
GAGGAGGAAG ATGAGGAAGA TGAAGATGAG GAGGAAGAAG AGGAATCGAG CGAAGCGGGA 
GACTATTTCC CCTGGGAGAG GGAGCTTCAG TCAGGCCTTT GGCTGGAAAA TCTGGCAAAT 
GAAGAGGATG TCAGTGTTTT TAAAGCTAGG AACCTGCAGA TCCAGCAAGC CATTCATCCA 
GTAGACCCTC TGGACATCCA GATGGAGAGC CATCAGGCTG AGGGGGTGTT GGAGATGCCC 
TCGGTGTCAC CCTTAGGCTC ACAGCCCTCG CTCAACACCC AGCTCACACA GCCCACCTCC 
TCCACAGCCC CCTCCCACAA ATCCGTACGT CACGAGGCTG TCAGAGCCTG GCTGGACTCG 
ATGTCTGGAG AGCCCTGTGA AGAGGACGAT GACCCTGAAG CTGAAGCAGG CAGTCCTGAC 
TTTGAGCCAG GCACTGAAAT AGATCAAGAG GACATTCCCT CAGATGCAGA GGCTGAAGCT 
CGTTCACGGT GCATAGATGA AGTGGTGACA CTTCCAGTGG ATAATGATAA AACAGAAAGC 
CAGGGACAGG TTTTCAACAC TGCAGAGAAA ACGTCTGCAA ATCCAAGGGA GTTGATTGTG 
GACGTTGTGC TGTCTCCAAT CCAAAAACCT GCTTTACCCA TTGAGGAGGT GCATGAAGTT 
TCTCCTGTAG TACTAGTTAA ATCTCCTGGT GCACGTTTCT TCCCTGAGCC CTATCTGCCT 
GATAAAGTCA AGCAGAACAT TCCCCCGCCT CAGAGTCCTG ATGTTAAAAC CCCTCATTCT 
CCTGTAGCTC AAATCATTGC GCTGTCGCCC ATCTGTTCCC AGCCTGTTCC ACAACCAGGC 
ACAGCATCTC CCAAATCTCC AGTCCAACCA CAGCCTTGTG CCTGTTCTCC AACAGGCAAT 
CCTTTGTCTC CGATTTGTAC CCAATCGCAA CCCTGCAATG AGCCACCTTC ACCCCTATCC 
ACAAGCTCCC CCGTCAGGAC TCAACCAGTC CCAGCAGTAA CTTCTACTCC TTTAGCTAAA 
CCTGCTTCCG AGAATAGGAC TAATGAACAT TTGAAAGATT CAACTCCTGA GCTTAAGAAA 
ACAGACCTCA TTGAGGAGTT CTGGTTGAAG AGTGCAGAGA TTAGAAAAAG TTTAGGGCTC 
ACTCCCCTGG AACGAAGCAA AACTGCGGTG GAGAAGAGCA TTGTGAAAAC TCCAACACCC 
GAATCATCAT CTCCTAAGTC TTATACCCCA GAGGACTTGT CTGAGGAACA GAAGCCTACA 
TTTACGGGCC GTTCTATCAT ACGCAGGATC AATATTACCT TGGAGGGTCA GGTTATATCT 
CCGGTGGAAC CTAAAAGTAA TGGTTCAGAA AAGAAGGACT TAAGCAGCAG TTCAGGGTTG 
GGTCTAAACG GAAGTGTGAC CACCAGTCAG ACAGCTGCTA GCGACAGCTA CAACAACTCT 
GATTCTACAA TGCTTACCCC TCCTTCCAGT CCACCACCTC CTCCACCCAG GGAGGAACCT 
GCTTGTCTCC AAAACAAGAA GTCTCAGGTT TCCTGGGACA ACCTCCTTGA AGGCACAGAA 
GAACCCAAAT CTGAGACCAT GCCCATTAAG CCCAGGACTC CAGTTAGTCC CCCTCAACCA 
AAACAAAAAC CAGTTACAGC TCCAGTGCCA ACCCCTAGAA CCAATCCACC TGTTGTGATG 
AGGGTTAAGG AGCCCAATAA ACCACGTCGA GAAGAAGTGA GAAAGTCTTT TGTTGAGTGT 
GTAGACGAGA TACCATTTGC TGATGATGTT GAAGACACAT ACGATGACCG CACTCCAGAT 
GCAAGTGGTC TTGAAAAGTT TTACACACCG CCGACTAGCA AGGTCAACCG GGATAAGCCG 
CCTCTCCACT TGGCACTGGC TATGGAAAAT GGCAAGCCCA ATATCCCTGG AGTTTCCCGA 
ACAGCAAAGG GTTCCCAGCA TTTTTCTCCT GAAGCCAAGG AGATCGCAGA GGAGCGAATG 
AGGGCACGGG AAAAGTCTGT GAAGAGCCAA GCTCTTAAAG ATGCAATGGC CAAGCAGCTT 
ACAAAGATGA AAGATTCTGA GGTTGCCAAG GGGGCTGTAG CAAAAGTAGC CTGGGATATT 
CCTGAAACAA AAGGAAAAAG CAAAAAGCAA TCTAAAGCCC AGAAGGACTC TGCAGTGAAG 
GCCTTGGAGT CCAAAAAGCA AGCAGACACA CTTCCAGACC GTTTCTTCTC TACACCATCT 
AGTAAGGCCT TAGACAGCTC TGTCACATCT TCAGAGAGCT CCACAGGGGG CAAAAGCAAG 
AAACGCAGCT CACTGTTTTC TCCACGCAAA AACAAAAAAG AAAAGAAAGC CAAAAATGAA 
AGACTGTCCA GCACTGAAGA AACGCCGCCC AAGCACAAGT CCCTTTGGAA AGCAGTGTTC 
TCCGGCTACA AGAAGGACAA AAAGAAGAAA GATGACAAGT CATGCCCAAG TACACCCTCA 
AGCTCTACAA CTGGAGACTC GGGGAAGAAG AAAGATTCAC CCCTGGACAG GTCCTCAGAT 
TTACGTCTGA GGAGAAATCT AAGCTTTTCT GAGGACTCTG ATCTCTCCTG TGACGATGTT 
CTGGAAAGAT CCTCTCAGAA GTCCAAGGGT GATCGTCATG TGGACCTGTT TGACTTGGTG 
TCAGATTTGA AAAAAGAAGC CATTAAAGAG GAGAAAGCAA AAGAGAAGGC TAAGGATAGG 
GCAAAGGAAA GGGGAAGAGT TACTGAGAAG GGCAAGGAAA GGTCAAGAGA AAGGGAGAAT 
GAAAAAAGAG TCAAAGAAAA GATGGTGAAA ATGAAAGATG TGGAACGGGA GAAAGGAAAA 
GAGGAAGAGT CTGTTTATGT ACCTCATGCA TTGGCGTTCA AGAGATCATA TGCCACAAAG 
AAGACATACA CGGAGGAGGA ACTGAACGCC AAGCTGACAA GAAGAGTACA GAAAGCTGCA 
CGGAGGCAGG CCAAGCAGGA GGAGCTCAAG AGACTTCACA GAGCTCAGAT GATTCAGAGG 
CAGCTGGAGC AGGTGGAAGA GAAACAGAGG CAGTTAGAGG AGAGAGGCGT CGCGGTGGAA 
AAGGCACTGC GAGGCGAAGC AGGGTTGTAT AAAGGTTCTA GCGTTAGTCC AAAACAGCGT 
AAGAGACGCT CAGACTATTG GGGAGAGTCT AATTACAGTG AGATCCTGGA TTTGCATCTG 
GGCGTTGAGC CCTATGTCGG GACCCCTCGC CGAAGAGCCC TGTACCTCTG CCCCTGCTGT 
GCCCCTGAAG GAATGGGTAA AAAGGACGAT CCGAAGCTGA TGCAGGAATG GTTTAAACTG 
GTCCAGGAGA AGAATGCCTT GGTACGATAT GAATCCGAGC TGATGATATT TGCTCGGGAG 
CTAGAGTTGG AGGACAGACA GAGTCGACTG CAACAGGAAC TACGGGAACG CATGGCAATA 
GATGATCATC TGAAGACAGA GGAGGAGCTG GCAGAGGAGA AGCAGATTCT GAATGAGATG 
TTGGAGGTGG TGGAGCAGCG AGACTCTCTG GTGGCCCTGC TGGAGGAACA GAGACTCAGG 
GAGAAGGAAG AAGATAAAGA CCTGGAGGCG GTGATGCTCT CCAAAGGATT CAACCTCAAC 
TGGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323435.1
CDS	604..8172
Translation	MGDGGVNAVGEGVNQSHMLFDRFVQATTCKGTLKAFQELCDFLELKPNEYRVFYHKLKSKLNYWKAKALWAKLDKRASHKEYKKGRACANTKCLIIGAGPCGLRTAIELGFLGAKVVLLEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGIEIHVNVEFKGLIEPPEDQENERIGWRAEVHPRTHPVNELEFDVIIGADGRRNTLSGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILHDYADTEMLLSRANVDQKALLSYAREAADFSTNHQLPKLDFAINHYGQPDVAMFDFTCMYASENAALVRQRNGHKLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWAHGSSPLEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNISLHQVRPNQVRHLLDTGETRDLRVDLENVVNSSTPKLTRNEILLEKQLQESIVRSSKLLNWCQRQTEGYRGVSVSDLTTSWKSGLALCALIHRYRPDLIDFESLDEKDVEKNNQLAFDVAEREFGISPIMTGKEMSVVVEPDKLSMVMYLSQFYEMFKDTVPPGENQNLSPEEKAALIASTKSPISFLSKLGQSIAISRKRNPKDKKEKELDGLGKRRKTSQAGQSEDEELQRANRDDRPSIATALAERKIDSAAAANNNNKVKSMATQLLAKFEENAPTQSTGLKRQGDSMPDLGLILTPSPAAAPLNESVRLAPVPAWRKRRTQLQEQMSFRYKEKLKCQTLPITQEQASSGTEVLRSCPKKTILLSSSSPSSSSFSSSFPPHAQSVIHKCNGVGDGENRFQDLSVCYWEDNLPNVDKPEPIHIRSIQERAETLVARFKGKADRKDKPQVMKKPSRFFIEQWFLTHGLSPDEPVFSSDPQHHQQKGVLCPDDHVPLHIPSIQERAERLASHFKDKPAKPKSKKKSSLLLTEQWSRARDVSSASPLHSPSSFRQHCVKMYTGGVSSLAEQIANQLQAKDDPRPMLEKRDLGSFRKEFPQNIGGSDVCFFCRKRVYVMERLSAEGKFFHRSCFKCDYCGTTLRLSSYAFDVEDGKFYCKPHYCYRLSGVAQRKRPAPAAAPANAKEPQVLAVSPNTVDAPGQAITTQTPAERRPSETEVNGVTEPSVAKRLKGTPERIELENYRLSMMREEELEEVPEETLAEHNLSSVLDKATDIEEGSSSSESDMEEEDEDAEAAGPSDLGGVPWKEAVELHAKLKGESDPGADDDGLHDGDGEMDEDEEEEEDEEDEDEEEEEESSEAGDYFPWERELQSGLWLENLANEEDVSVFKARNLQIQQAIHPVDPLDIQMESHQAEGVLEMPSVSPLGSQPSLNTQLTQPTSSTAPSHKSVRHEAVRAWLDSMSGEPCEEDDDPEAEAGSPDFEPGTEIDQEDIPSDAEAEARSRCIDEVVTLPVDNDKTESQGQVFNTAEKTSANPRELIVDVVLSPIQKPALPIEEVHEVSPVVLVKSPGARFFPEPYLPDKVKQNIPPPQSPDVKTPHSPVAQIIALSPICSQPVPQPGTASPKSPVQPQPCACSPTGNPLSPICTQSQPCNEPPSPLSTSSPVRTQPVPAVTSTPLAKPASENRTNEHLKDSTPELKKTDLIEEFWLKSAEIRKSLGLTPLERSKTAVEKSIVKTPTPESSSPKSYTPEDLSEEQKPTFTGRSIIRRINITLEGQVISPVEPKSNGSEKKDLSSSSGLGLNGSVTTSQTAASDSYNNSDSTMLTPPSSPPPPPPREEPACLQNKKSQVSWDNLLEGTEEPKSETMPIKPRTPVSPPQPKQKPVTAPVPTPRTNPPVVMRVKEPNKPRREEVRKSFVECVDEIPFADDVEDTYDDRTPDASGLEKFYTPPTSKVNRDKPPLHLALAMENGKPNIPGVSRTAKGSQHFSPEAKEIAEERMRAREKSVKSQALKDAMAKQLTKMKDSEVAKGAVAKVAWDIPETKGKSKKQSKAQKDSAVKALESKKQADTLPDRFFSTPSSKALDSSVTSSESSTGGKSKKRSSLFSPRKNKKEKKAKNERLSSTEETPPKHKSLWKAVFSGYKKDKKKKDDKSCPSTPSSSTTGDSGKKKDSPLDRSSDLRLRRNLSFSEDSDLSCDDVLERSSQKSKGDRHVDLFDLVSDLKKEAIKEEKAKEKAKDRAKERGRVTEKGKERSRERENEKRVKEKMVKMKDVEREKGKEEESVYVPHALAFKRSYATKKTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQMIQRQLEQVEEKQRQLEERGVAVEKALRGEAGLYKGSSVSPKQRKRRSDYWGESNYSEILDLHLGVEPYVGTPRRRALYLCPCCAPEGMGKKDDPKLMQEWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAIDDHLKTEEELAEEKQILNEMLEVVEQRDSLVALLEEQRLREKEEDKDLEAVMLSKGFNLNWA

Target ORF information:

RefSeq Version	XM_021467760.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467760.1

ORF Insert Sequence:

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAAATT 
CTGCTTGAGA AGCAATTGCA AGAGTCCATC GTGCGCTCCA GTAAGCTGCT GAACTGGTGT 
CAGAGGCAGA CGGAAGGCTA CCGAGGTGTC AGCGTGTCTG ATCTCACCAC GTCCTGGAAG 
AGTGGTTTGG CCCTGTGCGC GCTCATCCAC AGATACAGAC CAGACCTCAT TGACTTTGAA 
TCACTGGACG AGAAAGACGT GGAGAAGAAC AACCAGCTGG CTTTCGACGT GGCTGAAAGA 
GAGTTTGGGA TTTCTCCCAT CATGACCGGG AAGGAGATGT CTGTTGTGGT GGAGCCAGAT 
AAGCTCTCCA TGGTGATGTA CCTCAGTCAG TTCTACGAGA TGTTCAAGGA CACGGTGCCA 
CCTGGTGAAA ACCAGAATCT GAGCCCTGAG GAGAAGGCCG CACTGATCGC CAGCACTAAA 
TCACCCATCT CATTCCTTAG TAAGCTAGGT CAAAGCATCG CAATCTCCCG CAAAAGAAAC 
CCCAAGGATA AAAAAGAAAA GGAGCTGGAT GGACTAGGGA AAAGACGAAA GACGAGCCAG 
GCTGGACAAT CGGAGGACGA GGAGCTGCAG AGGGCTAATC GAGACGACAG GCCATCTATA 
GCCACAGCTC TGGCAGAACG TAAGATCGAC TCCGCTGCAG CAGCCAACAA TAACAACAAA 
GTGAAGTCGA TGGCCACGCA GCTGCTGGCC AAGTTTGAGG AGAACGCGCC GACACAGTCC 
ACTGGGCTCA AACGACAGGG AGACTCTATG CCCGATCTGG GCCTCATACT GACCCCTTCT 
CCAGCCGCTG CTCCTCTGAA CGAGTCAGTT CGTCTGGCTC CAGTGCCTGC ATGGAGGAAG 
AGGCGCACAC AGTTACAGGA GCAGATGAGT TTCCGCTATA AAGAAAAACT CAAGTGTCAA 
ACTCTCCCCA TCACACAGGA GCAGGCCAGC AGTGGGACTG AGGTCTTGAG GAGCTGCCCT 
AAGAAAACCA TTCTTCTTTC TTCTTCCTCA CCCTCATCTT CCTCATTCTC TTCATCGTTT 
CCTCCACATG CACAGTCAGT GATACACAAG TGTAATGGTG TTGGTGATGG AGAGAACAGA 
TTCCAGGACC TTTCAGTCTG TTACTGGGAA GATAATCTGC CTAATGTGGA CAAACCAGAA 
CCCATACATA TTCGTAGCAT TCAAGAGCGA GCGGAGACTC TAGTGGCTAG ATTTAAAGGC 
AAGGCTGACA GGAAGGACAA GCCACAGGTT ATGAAAAAGC CATCACGGTT CTTTATTGAG 
CAGTGGTTTT TGACCCACGG CCTCAGTCCT GATGAGCCCG TCTTCTCCTC TGACCCTCAG 
CATCATCAGC AAAAAGGGGT TTTGTGTCCT GACGATCACG TGCCTTTACA CATCCCCAGC 
ATTCAGGAGA GAGCCGAGAG ACTGGCCTCT CACTTTAAAG ACAAACCGGC GAAGCCAAAG 
TCTAAGAAAA AGTCGTCACT GTTGTTGACA GAGCAGTGGT CCAGGGCTCG TGATGTGAGC 
TCTGCTAGTC CTCTGCATTC CCCCAGCTCT TTCCGGCAGC ACTGTGTAAA GATGTACACA 
GGGGGTGTGA GCTCATTGGC TGAGCAAATA GCCAATCAGC TTCAGGCTAA GGACGACCCC 
AGACCCATGC TTGAGAAAAG GGATTTGGGT TCCTTTCGAA AGGAGTTCCC TCAGAACATC 
GGTGGCAGCG ATGTGTGTTT CTTCTGCCGT AAACGGGTCT ATGTGATGGA GCGTCTCAGC 
GCTGAAGGAA AGTTTTTCCA TCGCAGCTGC TTCAAGTGCG ACTACTGCGG CACAACCCTC 
CGCCTGTCCT CATATGCCTT CGATGTTGAA GACGGGAAGT TTTATTGTAA ACCACACTAC 
TGCTACCGAC TCTCTGGTGT GGCACAGAGA AAACGGCCTG CACCTGCTGC TGCTCCAGCA 
AACGCCAAGG AGCCCCAGGT GTTGGCGGTA TCCCCCAACA CAGTGGACGC CCCAGGTCAA 
GCCATAACCA CTCAGACCCC TGCGGAGCGC CGGCCCTCAG AAACTGAGGT AAATGGTGTC 
ACGGAGCCCA GCGTGGCCAA ACGTCTGAAA GGGACTCCGG AAAGGATCGA GCTGGAGAAC 
TATCGTTTGT CAATGATGAG GGAGGAGGAG CTGGAGGAGG TGCCGGAGGA GACATTGGCG 
GAGCACAACC TCAGCAGTGT GCTGGATAAA GCCACCGACA TCGAGGAGGG ATCCAGCAGC 
TCTGAGTCTG ACATGGAGGA AGAGGATGAA GATGCAGAGG CGGCCGGTCC GTCTGATCTG 
GGTGGAGTTC CCTGGAAGGA GGCAGTAGAG CTCCATGCTA AGTTAAAGGG AGAAAGCGAC 
CCAGGAGCTG ACGATGATGG CCTTCATGAC GGTGATGGAG AGATGGACGA GGATGAAGAA 
GAGGAGGAAG ATGAGGAAGA TGAAGATGAG GAGGAAGAAG AGGAATCGAG CGAAGCGGGA 
GACTATTTCC CCTGGGAGAG GGAGCTTCAG TCAGGCCTTT GGCTGGAAAA TCTGGCAAAT 
GAAGAGGATG TCAGTGTTTT TAAAGCTAGG AACCTGCAGA TCCAGCAAGC CATTCATCCA 
GTAGACCCTC TGGACATCCA GATGGAGAGC CATCAGGCTG AGGGGGTGTT GGAGATGCCC 
TCGGTGTCAC CCTTAGGCTC ACAGCCCTCG CTCAACACCC AGCTCACACA GCCCACCTCC 
TCCACAGCCC CCTCCCACAA ATCCGTACGT CACGAGGCTG TCAGAGCCTG GCTGGACTCG 
ATGTCTGGAG AGCCCTGTGA AGAGGACGAT GACCCTGAAG CTGAAGCAGG CAGTCCTGAC 
TTTGAGCCAG GCACTGAAAT AGATCAAGAG GACATTCCCT CAGATGCAGA GGCTGAAGCT 
CGTTCACGGT GCATAGATGA AGTGGTGACA CTTCCAGTGG ATAATGATAA AACAGAAAGC 
CAGGGACAGG TTTTCAACAC TGCAGAGAAA ACGTCTGCAA ATCCAAGGGA GTTGATTGTG 
GACGTTGTGC TGTCTCCAAT CCAAAAACCT GCTTTACCCA TTGAGGAGGT GCATGAAGTT 
TCTCCTGTAG TACTAGTTAA ATCTCCTGGT GCACGTTTCT TCCCTGAGCC CTATCTGCCT 
GATAAAGTCA AGCAGAACAT TCCCCCGCCT CAGAGTCCTG ATGTTAAAAC CCCTCATTCT 
CCTGTAGCTC AAATCATTGC GCTGTCGCCC ATCTGTTCCC AGCCTGTTCC ACAACCAGGC 
ACAGCATCTC CCAAATCTCC AGTCCAACCA CAGCCTTGTG CCTGTTCTCC AACAGGCAAT 
CCTTTGTCTC CGATTTGTAC CCAATCGCAA CCCTGCAATG AGCCACCTTC ACCCCTATCC 
ACAAGCTCCC CCGTCAGGAC TCAACCAGTC CCAGCAGTAA CTTCTACTCC TTTAGCTAAA 
CCTGCTTCCG AGAATAGGAC TAATGAACAT TTGAAAGATT CAACTCCTGA GCTTAAGAAA 
ACAGACCTCA TTGAGGAGTT CTGGTTGAAG AGTGCAGAGA TTAGAAAAAG TTTAGGGCTC 
ACTCCCCTGG AACGAAGCAA AACTGCGGTG GAGAAGAGCA TTGTGAAAAC TCCAACACCC 
GAATCATCAT CTCCTAAGTC TTATACCCCA GAGGACTTGT CTGAGGAACA GAAGCCTACA 
TTTACGGGCC GTTCTATCAT ACGCAGGATC AATATTACCT TGGAGGGTCA GGTTATATCT 
CCGGTGGAAC CTAAAAGTAA TGGTTCAGAA AAGAAGGACT TAAGCAGCAG TTCAGGGTTG 
GGTCTAAACG GAAGTGTGAC CACCAGTCAG ACAGCTGCTA GCGACAGCTA CAACAACTCT 
GATTCTACAA TGCTTACCCC TCCTTCCAGT CCACCACCTC CTCCACCCAG GGAGGAACCT 
GCTTGTCTCC AAAACAAGAA GTCTCAGGTT TCCTGGGACA ACCTCCTTGA AGGCACAGAA 
GAACCCAAAT CTGAGACCAT GCCCATTAAG CCCAGGACTC CAGTTAGTCC CCCTCAACCA 
AAACAAAAAC CAGTTACAGC TCCAGTGCCA ACCCCTAGAA CCAATCCACC TGTTGTGATG 
AGGGTTAAGG AGCCCAATAA ACCACGTCGA GAAGAAGTGA GAAAGTCTTT TGTTGAGTGT 
GTAGACGAGA TACCATTTGC TGATGATGTT GAAGACACAT ACGATGACCG CACTCCAGAT 
GCAAGTGGTC TTGAAAAGTT TTACACACCG CCGACTAGCA AGGTCAACCG GGATAAGCCG 
CCTCTCCACT TGGCACTGGC TATGGAAAAT GGCAAGCCCA ATATCCCTGG AGTTTCCCGA 
ACAGCAAAGG GTTCCCAGCA TTTTTCTCCT GAAGCCAAGG AGATCGCAGA GGAGCGAATG 
AGGGCACGGG AAAAGTCTGT GAAGAGCCAA GCTCTTAAAG ATGCAATGGC CAAGCAGCTT 
ACAAAGATGA AAGATTCTGA GGTTGCCAAG GGGGCTGTAG CAAAAGTAGC CTGGGATATT 
CCTGAAACAA AAGGAAAAAG CAAAAAGCAA TCTAAAGCCC AGAAGGACTC TGCAGTGAAG 
GCCTTGGAGT CCAAAAAGCA AGCAGACACA CTTCCAGACC GTTTCTTCTC TACACCATCT 
AGTAAGGCCT TAGACAGCTC TGTCACATCT TCAGAGAGCT CCACAGGGGG CAAAAGCAAG 
AAACGCAGCT CACTGTTTTC TCCACGCAAA AACAAAAAAG AAAAGAAAGC CAAAAATGAA 
AGACTGTCCA GCACTGAAGA AACGCCGCCC AAGCACAAGT CCCTTTGGAA AGCAGTGTTC 
TCCGGCTACA AGAAGGACAA AAAGAAGAAA GATGACAAGT CATGCCCAAG TACACCCTCA 
AGCTCTACAA CTGGAGACTC GGGGAAGAAG AAAGATTCAC CCCTGGACAG GTCCTCAGAT 
TTACGTCTGA GGAGAAATCT AAGCTTTTCT GAGGACTCTG ATCTCTCCTG TGACGATGTT 
CTGGAAAGAT CCTCTCAGAA GTCCAAGGGT GATCGTCATG TGGACCTGTT TGACTTGGTG 
TCAGATTTGA AAAAAGAAGC CATTAAAGAG GAGAAAGCAA AAGAGAAGGC TAAGGATAGG 
GCAAAGGAAA GGGGAAGAGT TACTGAGAAG GGCAAGGAAA GGTCAAGAGA AAGGGAGAAT 
GAAAAAAGAG TCAAAGAAAA GATGGTGAAA ATGAAAGATG TGGAACGGGA GAAAGGAAAA 
GAGGAAGAGT CTGTTTATGT ACCTCATGCA TTGGCGTTCA AGAGATCATA TGCCACAAAG 
AAGACATACA CGGAGGAGGA ACTGAACGCC AAGCTGACAA GAAGAGTACA GAAAGCTGCA 
CGGAGGCAGG CCAAGCAGGA GGAGCTCAAG AGACTTCACA GAGCTCAGAT GATTCAGAGG 
CAGCTGGAGC AGGTGGAAGA GAAACAGAGG CAGTTAGAGG AGAGAGGCGT CGCGGTGGAA 
AAGGCACTGC GAGGCGAAGC AGGGTTGTAT AAAGGTTCTA GCGTTAGTCC AAAACAGCGT 
AAGAGACGCT CAGACTATTG GGGAGAGTCT AATTACAGTG AGATCCTGGA TTTGCATCTG 
GGCGTTGAGC CCTATGTCGG GACCCCTCGC CGAAGAGCCC TGTACCTCTG CCCCTGCTGT 
GCCCCTGAAG GAATGGGTAA AAAGGACGAT CCGAAGCTGA TGCAGGAATG GTTTAAACTG 
GTCCAGGAGA AGAATGCCTT GGTACGATAT GAATCCGAGC TGATGATATT TGCTCGGGAG 
CTAGAGTTGG AGGACAGACA GAGTCGACTG CAACAGGAAC TACGGGAACG CATGGCAATA 
GATGATCATC TGAAGACAGA GGAGGAGCTG GCAGAGGAGA AGCAGATTCT GAATGAGATG 
TTGGAGGTGG TGGAGCAGCG AGACTCTCTG GTGGCCCTGC TGGAGGAACA GAGACTCAGG 
GAGAAGGAAG AAGATAAAGA CCTGGAGGCG GTGATGCTCT CCAAAGGATT CAACCTCAAC 
TGGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3A ( XM_021473238.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021328913.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X5, mRNA.

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CloneID	ODa145982
Clone ID Related Accession (Same CDS sequence)	XM_021473238.1 , XM_021467761.1
Accession Version	XM_021473238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3a isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395007.1) and transcript sequence (GFIL01026059.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1201 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAGTCC 
ATCGTGCGCT CCAGTAAGCT GCTGAACTGG TGTCAGAGGC AGACGGAAGG CTACCGAGGT 
GTCAGCGTGT CTGATCTCAC CACGTCCTGG AAGAGTGGTT TGGCCCTGTG CGCGCTCATC 
CACAGATACA GACCAGACCT CATTGACTTT GAATCACTGG ACGAGAAAGA CGTGGAGAAG 
AACAACCAGC TGGCTTTCGA CGTGGCTGAA AGAGAGTTTG GGATTTCTCC CATCATGACC 
GGGAAGGAGA TGTCTGTTGT GGTGGAGCCA GATAAGCTCT CCATGGTGAT GTACCTCAGT 
CAGTTCTACG AGATGTTCAA GGACACGGTG CCACCTGGTG AAAACCAGAA TCTGAGCCCT 
GAGGAGAAGG CCGCACTGAT CGCCAGCACT AAATCACCCA TCTCATTCCT TAGTAAGCTA 
GGTCAAAGCA TCGCAATCTC CCGCAAAAGA AACCCCAAGG ATAAAAAAGA AAAGGAGCTG 
GATGGACTAG GGAAAAGACG AAAGACGAGC CAGGCTGGAC AATCGGAGGA CGAGGAGCTG 
CAGAGGGCTA ATCGAGACGA CAGGCCATCT ATAGCCACAG CTCTGGCAGA ACGTAAGATC 
GACTCCGCTG CAGCAGCCAA CAATAACAAC AAAGTGAAGT CGATGGCCAC GCAGCTGCTG 
GCCAAGTTTG AGGAGAACGC GCCGACACAG TCCACTGGGC TCAAACGACA GGGTTCCTTT 
CGAAAGGAGT TCCCTCAGAA CATCGGTGGC AGCGATGTGT GTTTCTTCTG CCGTAAACGG 
GTCTATGTGA TGGAGCGTCT CAGCGCTGAA GGAAAGTTTT TCCATCGCAG CTGCTTCAAG 
TGCGACTACT GCGGCACAAC CCTCCGCCTG TCCTCATATG CCTTCGATGT TGAAGACGGG 
AAGTTTTATT GTAAACCACA CTACTGCTAC CGACTCTCTG GTGTGGCACA GAGAAAACGG 
CCTGCACCTG CTGCTGCTCC AGCAAACGCC AAGGAGCCCC AGGTGTTGGC GGTATCCCCC 
AACACAGTGG ACGCCCCAGG TCAAGCCATA ACCACTCAGA CCCCTGCGGA GCGCCGGCCC 
TCAGAAACTG AGGTAAATGG TGTCACGGAG CCCAGCGTGG CCAAACGTCT GAAAGGGACT 
CCGGAAAGGA TCGAGCTGGA GAACTATCGT TTGTCAATGA TGAGGGAGGA GGAGCTGGAG 
GAGGTGCCGG AGGAGACATT GGCGGAGCAC AACCTCAGCA GTGTGCTGGA TAAAGCCACC 
GACATCGAGG AGGGATCCAG CAGCTCTGAG TCTGACATGG AGGAAGAGGA TGAAGATGCA 
GAGGCGGCCG GTCCGTCTGA TCTGGGTGGA GTTCCCTGGA AGGAGGCAGT AGAGCTCCAT 
GCTAAGTTAA AGGGAGAAAG CGACCCAGGA GCTGACGATG ATGGCCTTCA TGACGGTGAT 
GGAGAGATGG ACGAGGATGA AGAAGAGGAG GAAGATGAGG AAGATGAAGA TGAGGAGGAA 
GAAGAGGAAT CGAGCGAAGC GGGAGACTAT TTCCCCTGGG AGAGGGAGCT TCAGTCAGGC 
CTTTGGCTGG AAAATCTGGC AAATGAAGAG GATGTCAGTG TTTTTAAAGC TAGGAACCTG 
CAGATCCAGC AAGCCATTCA TCCAGTAGAC CCTCTGGACA TCCAGATGGA GAGCCATCAG 
GCTGAGGGGG TGTTGGAGAT GCCCTCGGTG TCACCCTTAG GCTCACAGCC CTCGCTCAAC 
ACCCAGCTCA CACAGCCCAC CTCCTCCACA GCCCCCTCCC ACAAATCCGT ACGTCACGAG 
GCTGTCAGAG CCTGGCTGGA CTCGATGTCT GGAGAGCCCT GTGAAGAGGA CGATGACCCT 
GAAGCTGAAG CAGGCAGTCC TGACTTTGAG CCAGGCACTG AAATAGATCA AGAGGACATT 
CCCTCAGATG CAGAGGCTGA AGCTCGTTCA CGGTGCATAG ATGAAGTGGT GACACTTCCA 
GTGGATAATG ATAAAACAGA AAGCCAGGGA CAGGTTTTCA ACACTGCAGA GAAAACGTCT 
GCAAATCCAA GGGAGTTGAT TGTGGACGTT GTGCTGTCTC CAATCCAAAA ACCTGCTTTA 
CCCATTGAGG AGGTGCATGA AGTTTCTCCT GTAGTACTAG TTAAATCTCC TGGTGCACGT 
TTCTTCCCTG AGCCCTATCT GCCTGATAAA GTCAAGCAGA ACATTCCCCC GCCTCAGAGT 
CCTGATGTTA AAACCCCTCA TTCTCCTGTA GCTCAAATCA TTGCGCTGTC GCCCATCTGT 
TCCCAGCCTG TTCCACAACC AGGCACAGCA TCTCCCAAAT CTCCAGTCCA ACCACAGCCT 
TGTGCCTGTT CTCCAACAGG CAATCCTTTG TCTCCGATTT GTACCCAATC GCAACCCTGC 
AATGAGCCAC CTTCACCCCT ATCCACAAGC TCCCCCGTCA GGACTCAACC AGTCCCAGCA 
GTAACTTCTA CTCCTTTAGC TAAACCTGCT TCCGAGAATA GGACTAATGA ACATTTGAAA 
GATTCAACTC CTGAGCTTAA GAAAACAGAC CTCATTGAGG AGTTCTGGTT GAAGAGTGCA 
GAGATTAGAA AAAGTTTAGG GCTCACTCCC CTGGAACGAA GCAAAACTGC GGTGGAGAAG 
AGCATTGTGA AAACTCCAAC ACCCGAATCA TCATCTCCTA AGTCTTATAC CCCAGAGGAC 
TTGTCTGAGG AACAGAAGCC TACATTTACG GGCCGTTCTA TCATACGCAG GATCAATATT 
ACCTTGGAGG GTCAGGTTAT ATCTCCGGTG GAACCTAAAA GTAATGGTTC AGAAAAGAAG 
GACTTAAGCA GCAGTTCAGG GTTGGGTCTA AACGGAAGTG TGACCACCAG TCAGACAGCT 
GCTAGCGACA GCTACAACAA CTCTGATTCT ACAATGCTTA CCCCTCCTTC CAGTCCACCA 
CCTCCTCCAC CCAGGGAGGA ACCTGCTTGT CTCCAAAACA AGAAGTCTCA GGTTTCCTGG 
GACAACCTCC TTGAAGGCAC AGAAGAACCC AAATCTGAGA CCATGCCCAT TAAGCCCAGG 
ACTCCAGTTA GTCCCCCTCA ACCAAAACAA AAACCAGTTA CAGCTCCAGT GCCAACCCCT 
AGAACCAATC CACCTGTTGT GATGAGGGTT AAGGAGCCCA ATAAACCACG TCGAGAAGAA 
GTGAGAAAGT CTTTTGTTGA GTGTGTAGAC GAGATACCAT TTGCTGATGA TGTTGAAGAC 
ACATACGATG ACCGCACTCC AGATGCAAGT GGTCTTGAAA AGTTTTACAC ACCGCCGACT 
AGCAAGGTCA ACCGGGATAA GCCGCCTCTC CACTTGGCAC TGGCTATGGA AAATGGCAAG 
CCCAATATCC CTGGAGTTTC CCGAACAGCA AAGGGTTCCC AGCATTTTTC TCCTGAAGCC 
AAGGAGATCG CAGAGGAGCG AATGAGGGCA CGGGAAAAGT CTGTGAAGAG CCAAGCTCTT 
AAAGATGCAA TGGCCAAGCA GCTTACAAAG ATGAAAGATT CTGAGGTTGC CAAGGGGGCT 
GTAGCAAAAG TAGCCTGGGA TATTCCTGAA ACAAAAGGAA AAAGCAAAAA GCAATCTAAA 
GCCCAGAAGG ACTCTGCAGT GAAGGCCTTG GAGTCCAAAA AGCAAGCAGA CACACTTCCA 
GACCGTTTCT TCTCTACACC ATCTAGTAAG GCCTTAGACA GCTCTGTCAC ATCTTCAGAG 
AGCTCCACAG GGGGCAAAAG CAAGAAACGC AGCTCACTGT TTTCTCCACG CAAAAACAAA 
AAAGAAAAGA AAGCCAAAAA TGAAAGACTG TCCAGCACTG AAGAAACGCC GCCCAAGCAC 
AAGTCCCTTT GGAAAGCAGT GTTCTCCGGC TACAAGAAGG ACAAAAAGAA GAAAGATGAC 
AAGTCATGCC CAAGTACACC CTCAAGCTCT ACAACTGGAG ACTCGGGGAA GAAGAAAGAT 
TCACCCCTGG ACAGGTCCTC AGATTTACGT CTGAGGAGAA ATCTAAGCTT TTCTGAGGAC 
TCTGATCTCT CCTGTGACGA TGTTCTGGAA AGATCCTCTC AGAAGTCCAA GGGTGATCGT 
CATGTGGACC TGTTTGACTT GGTGTCAGAT TTGAAAAAAG AAGCCATTAA AGAGGAGAAA 
GCAAAAGAGA AGGCTAAGGA TAGGGCAAAG GAAAGGGGAA GAGTTACTGA GAAGGGCAAG 
GAAAGGTCAA GAGAAAGGGA GAATGAAAAA AGAGTCAAAG AAAAGATGGT GAAAATGAAA 
GATGTGGAAC GGGAGAAAGG AAAAGAGGAA GAGTCTGTTT ATGTACCTCA TGCATTGGCG 
TTCAAGAGAT CATATGCCAC AAAGAAGACA TACACGGAGG AGGAACTGAA CGCCAAGCTG 
ACAAGAAGAG TACAGAAAGC TGCACGGAGG CAGGCCAAGC AGGAGGAGCT CAAGAGACTT 
CACAGAGCTC AGATGATTCA GAGGCAGCTG GAGCAGGTGG AAGAGAAACA GAGGCAGTTA 
GAGGAGAGAG GCGTCGCGGT GGAAAAGGCA CTGCGAGGCG AAGCAGGAAT GGGTAAAAAG 
GACGATCCGA AGCTGATGCA GGAATGGTTT AAACTGGTCC AGGAGAAGAA TGCCTTGGTA 
CGATATGAAT CCGAGCTGAT GATATTTGCT CGGGAGCTAG AGTTGGAGGA CAGACAGAGT 
CGACTGCAAC AGGAACTACG GGAACGCATG GCAATAGATG ATCATCTGAA GACAGAGGAG 
GAGCTGGCAG AGGAGAAGCA GATTCTGAAT GAGATGTTGG AGGTGGTGGA GCAGCGAGAC 
TCTCTGGTGG CCCTGCTGGA GGAACAGAGA CTCAGGGAGA AGGAAGAAGA TAAAGACCTG 
GAGGCGGTGA TGCTCTCCAA AGGATTCAAC CTCAACTGGG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328913.1
CDS	349..6813
Translation	MGDGGVNAVGEGVNQSHMLFDRFVQATTCKGTLKAFQELCDFLELKPNEYRVFYHKLKSKLNYWKAKALWAKLDKRASHKEYKKGRACANTKCLIIGAGPCGLRTAIELGFLGAKVVLLEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGIEIHVNVEFKGLIEPPEDQENERIGWRAEVHPRTHPVNELEFDVIIGADGRRNTLSGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILHDYADTEMLLSRANVDQKALLSYAREAADFSTNHQLPKLDFAINHYGQPDVAMFDFTCMYASENAALVRQRNGHKLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWAHGSSPLEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNISLHQVRPNQVRHLLDTGETRDLRVDLENVVNSSTPKLTRNESIVRSSKLLNWCQRQTEGYRGVSVSDLTTSWKSGLALCALIHRYRPDLIDFESLDEKDVEKNNQLAFDVAEREFGISPIMTGKEMSVVVEPDKLSMVMYLSQFYEMFKDTVPPGENQNLSPEEKAALIASTKSPISFLSKLGQSIAISRKRNPKDKKEKELDGLGKRRKTSQAGQSEDEELQRANRDDRPSIATALAERKIDSAAAANNNNKVKSMATQLLAKFEENAPTQSTGLKRQGSFRKEFPQNIGGSDVCFFCRKRVYVMERLSAEGKFFHRSCFKCDYCGTTLRLSSYAFDVEDGKFYCKPHYCYRLSGVAQRKRPAPAAAPANAKEPQVLAVSPNTVDAPGQAITTQTPAERRPSETEVNGVTEPSVAKRLKGTPERIELENYRLSMMREEELEEVPEETLAEHNLSSVLDKATDIEEGSSSSESDMEEEDEDAEAAGPSDLGGVPWKEAVELHAKLKGESDPGADDDGLHDGDGEMDEDEEEEEDEEDEDEEEEEESSEAGDYFPWERELQSGLWLENLANEEDVSVFKARNLQIQQAIHPVDPLDIQMESHQAEGVLEMPSVSPLGSQPSLNTQLTQPTSSTAPSHKSVRHEAVRAWLDSMSGEPCEEDDDPEAEAGSPDFEPGTEIDQEDIPSDAEAEARSRCIDEVVTLPVDNDKTESQGQVFNTAEKTSANPRELIVDVVLSPIQKPALPIEEVHEVSPVVLVKSPGARFFPEPYLPDKVKQNIPPPQSPDVKTPHSPVAQIIALSPICSQPVPQPGTASPKSPVQPQPCACSPTGNPLSPICTQSQPCNEPPSPLSTSSPVRTQPVPAVTSTPLAKPASENRTNEHLKDSTPELKKTDLIEEFWLKSAEIRKSLGLTPLERSKTAVEKSIVKTPTPESSSPKSYTPEDLSEEQKPTFTGRSIIRRINITLEGQVISPVEPKSNGSEKKDLSSSSGLGLNGSVTTSQTAASDSYNNSDSTMLTPPSSPPPPPPREEPACLQNKKSQVSWDNLLEGTEEPKSETMPIKPRTPVSPPQPKQKPVTAPVPTPRTNPPVVMRVKEPNKPRREEVRKSFVECVDEIPFADDVEDTYDDRTPDASGLEKFYTPPTSKVNRDKPPLHLALAMENGKPNIPGVSRTAKGSQHFSPEAKEIAEERMRAREKSVKSQALKDAMAKQLTKMKDSEVAKGAVAKVAWDIPETKGKSKKQSKAQKDSAVKALESKKQADTLPDRFFSTPSSKALDSSVTSSESSTGGKSKKRSSLFSPRKNKKEKKAKNERLSSTEETPPKHKSLWKAVFSGYKKDKKKKDDKSCPSTPSSSTTGDSGKKKDSPLDRSSDLRLRRNLSFSEDSDLSCDDVLERSSQKSKGDRHVDLFDLVSDLKKEAIKEEKAKEKAKDRAKERGRVTEKGKERSRERENEKRVKEKMVKMKDVEREKGKEEESVYVPHALAFKRSYATKKTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQMIQRQLEQVEEKQRQLEERGVAVEKALRGEAGMGKKDDPKLMQEWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAIDDHLKTEEELAEEKQILNEMLEVVEQRDSLVALLEEQRLREKEEDKDLEAVMLSKGFNLNWA

Target ORF information:

RefSeq Version	XM_021473238.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473238.1

ORF Insert Sequence:

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAGTCC 
ATCGTGCGCT CCAGTAAGCT GCTGAACTGG TGTCAGAGGC AGACGGAAGG CTACCGAGGT 
GTCAGCGTGT CTGATCTCAC CACGTCCTGG AAGAGTGGTT TGGCCCTGTG CGCGCTCATC 
CACAGATACA GACCAGACCT CATTGACTTT GAATCACTGG ACGAGAAAGA CGTGGAGAAG 
AACAACCAGC TGGCTTTCGA CGTGGCTGAA AGAGAGTTTG GGATTTCTCC CATCATGACC 
GGGAAGGAGA TGTCTGTTGT GGTGGAGCCA GATAAGCTCT CCATGGTGAT GTACCTCAGT 
CAGTTCTACG AGATGTTCAA GGACACGGTG CCACCTGGTG AAAACCAGAA TCTGAGCCCT 
GAGGAGAAGG CCGCACTGAT CGCCAGCACT AAATCACCCA TCTCATTCCT TAGTAAGCTA 
GGTCAAAGCA TCGCAATCTC CCGCAAAAGA AACCCCAAGG ATAAAAAAGA AAAGGAGCTG 
GATGGACTAG GGAAAAGACG AAAGACGAGC CAGGCTGGAC AATCGGAGGA CGAGGAGCTG 
CAGAGGGCTA ATCGAGACGA CAGGCCATCT ATAGCCACAG CTCTGGCAGA ACGTAAGATC 
GACTCCGCTG CAGCAGCCAA CAATAACAAC AAAGTGAAGT CGATGGCCAC GCAGCTGCTG 
GCCAAGTTTG AGGAGAACGC GCCGACACAG TCCACTGGGC TCAAACGACA GGGTTCCTTT 
CGAAAGGAGT TCCCTCAGAA CATCGGTGGC AGCGATGTGT GTTTCTTCTG CCGTAAACGG 
GTCTATGTGA TGGAGCGTCT CAGCGCTGAA GGAAAGTTTT TCCATCGCAG CTGCTTCAAG 
TGCGACTACT GCGGCACAAC CCTCCGCCTG TCCTCATATG CCTTCGATGT TGAAGACGGG 
AAGTTTTATT GTAAACCACA CTACTGCTAC CGACTCTCTG GTGTGGCACA GAGAAAACGG 
CCTGCACCTG CTGCTGCTCC AGCAAACGCC AAGGAGCCCC AGGTGTTGGC GGTATCCCCC 
AACACAGTGG ACGCCCCAGG TCAAGCCATA ACCACTCAGA CCCCTGCGGA GCGCCGGCCC 
TCAGAAACTG AGGTAAATGG TGTCACGGAG CCCAGCGTGG CCAAACGTCT GAAAGGGACT 
CCGGAAAGGA TCGAGCTGGA GAACTATCGT TTGTCAATGA TGAGGGAGGA GGAGCTGGAG 
GAGGTGCCGG AGGAGACATT GGCGGAGCAC AACCTCAGCA GTGTGCTGGA TAAAGCCACC 
GACATCGAGG AGGGATCCAG CAGCTCTGAG TCTGACATGG AGGAAGAGGA TGAAGATGCA 
GAGGCGGCCG GTCCGTCTGA TCTGGGTGGA GTTCCCTGGA AGGAGGCAGT AGAGCTCCAT 
GCTAAGTTAA AGGGAGAAAG CGACCCAGGA GCTGACGATG ATGGCCTTCA TGACGGTGAT 
GGAGAGATGG ACGAGGATGA AGAAGAGGAG GAAGATGAGG AAGATGAAGA TGAGGAGGAA 
GAAGAGGAAT CGAGCGAAGC GGGAGACTAT TTCCCCTGGG AGAGGGAGCT TCAGTCAGGC 
CTTTGGCTGG AAAATCTGGC AAATGAAGAG GATGTCAGTG TTTTTAAAGC TAGGAACCTG 
CAGATCCAGC AAGCCATTCA TCCAGTAGAC CCTCTGGACA TCCAGATGGA GAGCCATCAG 
GCTGAGGGGG TGTTGGAGAT GCCCTCGGTG TCACCCTTAG GCTCACAGCC CTCGCTCAAC 
ACCCAGCTCA CACAGCCCAC CTCCTCCACA GCCCCCTCCC ACAAATCCGT ACGTCACGAG 
GCTGTCAGAG CCTGGCTGGA CTCGATGTCT GGAGAGCCCT GTGAAGAGGA CGATGACCCT 
GAAGCTGAAG CAGGCAGTCC TGACTTTGAG CCAGGCACTG AAATAGATCA AGAGGACATT 
CCCTCAGATG CAGAGGCTGA AGCTCGTTCA CGGTGCATAG ATGAAGTGGT GACACTTCCA 
GTGGATAATG ATAAAACAGA AAGCCAGGGA CAGGTTTTCA ACACTGCAGA GAAAACGTCT 
GCAAATCCAA GGGAGTTGAT TGTGGACGTT GTGCTGTCTC CAATCCAAAA ACCTGCTTTA 
CCCATTGAGG AGGTGCATGA AGTTTCTCCT GTAGTACTAG TTAAATCTCC TGGTGCACGT 
TTCTTCCCTG AGCCCTATCT GCCTGATAAA GTCAAGCAGA ACATTCCCCC GCCTCAGAGT 
CCTGATGTTA AAACCCCTCA TTCTCCTGTA GCTCAAATCA TTGCGCTGTC GCCCATCTGT 
TCCCAGCCTG TTCCACAACC AGGCACAGCA TCTCCCAAAT CTCCAGTCCA ACCACAGCCT 
TGTGCCTGTT CTCCAACAGG CAATCCTTTG TCTCCGATTT GTACCCAATC GCAACCCTGC 
AATGAGCCAC CTTCACCCCT ATCCACAAGC TCCCCCGTCA GGACTCAACC AGTCCCAGCA 
GTAACTTCTA CTCCTTTAGC TAAACCTGCT TCCGAGAATA GGACTAATGA ACATTTGAAA 
GATTCAACTC CTGAGCTTAA GAAAACAGAC CTCATTGAGG AGTTCTGGTT GAAGAGTGCA 
GAGATTAGAA AAAGTTTAGG GCTCACTCCC CTGGAACGAA GCAAAACTGC GGTGGAGAAG 
AGCATTGTGA AAACTCCAAC ACCCGAATCA TCATCTCCTA AGTCTTATAC CCCAGAGGAC 
TTGTCTGAGG AACAGAAGCC TACATTTACG GGCCGTTCTA TCATACGCAG GATCAATATT 
ACCTTGGAGG GTCAGGTTAT ATCTCCGGTG GAACCTAAAA GTAATGGTTC AGAAAAGAAG 
GACTTAAGCA GCAGTTCAGG GTTGGGTCTA AACGGAAGTG TGACCACCAG TCAGACAGCT 
GCTAGCGACA GCTACAACAA CTCTGATTCT ACAATGCTTA CCCCTCCTTC CAGTCCACCA 
CCTCCTCCAC CCAGGGAGGA ACCTGCTTGT CTCCAAAACA AGAAGTCTCA GGTTTCCTGG 
GACAACCTCC TTGAAGGCAC AGAAGAACCC AAATCTGAGA CCATGCCCAT TAAGCCCAGG 
ACTCCAGTTA GTCCCCCTCA ACCAAAACAA AAACCAGTTA CAGCTCCAGT GCCAACCCCT 
AGAACCAATC CACCTGTTGT GATGAGGGTT AAGGAGCCCA ATAAACCACG TCGAGAAGAA 
GTGAGAAAGT CTTTTGTTGA GTGTGTAGAC GAGATACCAT TTGCTGATGA TGTTGAAGAC 
ACATACGATG ACCGCACTCC AGATGCAAGT GGTCTTGAAA AGTTTTACAC ACCGCCGACT 
AGCAAGGTCA ACCGGGATAA GCCGCCTCTC CACTTGGCAC TGGCTATGGA AAATGGCAAG 
CCCAATATCC CTGGAGTTTC CCGAACAGCA AAGGGTTCCC AGCATTTTTC TCCTGAAGCC 
AAGGAGATCG CAGAGGAGCG AATGAGGGCA CGGGAAAAGT CTGTGAAGAG CCAAGCTCTT 
AAAGATGCAA TGGCCAAGCA GCTTACAAAG ATGAAAGATT CTGAGGTTGC CAAGGGGGCT 
GTAGCAAAAG TAGCCTGGGA TATTCCTGAA ACAAAAGGAA AAAGCAAAAA GCAATCTAAA 
GCCCAGAAGG ACTCTGCAGT GAAGGCCTTG GAGTCCAAAA AGCAAGCAGA CACACTTCCA 
GACCGTTTCT TCTCTACACC ATCTAGTAAG GCCTTAGACA GCTCTGTCAC ATCTTCAGAG 
AGCTCCACAG GGGGCAAAAG CAAGAAACGC AGCTCACTGT TTTCTCCACG CAAAAACAAA 
AAAGAAAAGA AAGCCAAAAA TGAAAGACTG TCCAGCACTG AAGAAACGCC GCCCAAGCAC 
AAGTCCCTTT GGAAAGCAGT GTTCTCCGGC TACAAGAAGG ACAAAAAGAA GAAAGATGAC 
AAGTCATGCC CAAGTACACC CTCAAGCTCT ACAACTGGAG ACTCGGGGAA GAAGAAAGAT 
TCACCCCTGG ACAGGTCCTC AGATTTACGT CTGAGGAGAA ATCTAAGCTT TTCTGAGGAC 
TCTGATCTCT CCTGTGACGA TGTTCTGGAA AGATCCTCTC AGAAGTCCAA GGGTGATCGT 
CATGTGGACC TGTTTGACTT GGTGTCAGAT TTGAAAAAAG AAGCCATTAA AGAGGAGAAA 
GCAAAAGAGA AGGCTAAGGA TAGGGCAAAG GAAAGGGGAA GAGTTACTGA GAAGGGCAAG 
GAAAGGTCAA GAGAAAGGGA GAATGAAAAA AGAGTCAAAG AAAAGATGGT GAAAATGAAA 
GATGTGGAAC GGGAGAAAGG AAAAGAGGAA GAGTCTGTTT ATGTACCTCA TGCATTGGCG 
TTCAAGAGAT CATATGCCAC AAAGAAGACA TACACGGAGG AGGAACTGAA CGCCAAGCTG 
ACAAGAAGAG TACAGAAAGC TGCACGGAGG CAGGCCAAGC AGGAGGAGCT CAAGAGACTT 
CACAGAGCTC AGATGATTCA GAGGCAGCTG GAGCAGGTGG AAGAGAAACA GAGGCAGTTA 
GAGGAGAGAG GCGTCGCGGT GGAAAAGGCA CTGCGAGGCG AAGCAGGAAT GGGTAAAAAG 
GACGATCCGA AGCTGATGCA GGAATGGTTT AAACTGGTCC AGGAGAAGAA TGCCTTGGTA 
CGATATGAAT CCGAGCTGAT GATATTTGCT CGGGAGCTAG AGTTGGAGGA CAGACAGAGT 
CGACTGCAAC AGGAACTACG GGAACGCATG GCAATAGATG ATCATCTGAA GACAGAGGAG 
GAGCTGGCAG AGGAGAAGCA GATTCTGAAT GAGATGTTGG AGGTGGTGGA GCAGCGAGAC 
TCTCTGGTGG CCCTGCTGGA GGAACAGAGA CTCAGGGAGA AGGAAGAAGA TAAAGACCTG 
GAGGCGGTGA TGCTCTCCAA AGGATTCAAC CTCAACTGGG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3A ( XM_021467761.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021323436.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X2, mRNA.

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CloneID	ODa145982
Clone ID Related Accession (Same CDS sequence)	XM_021473238.1 , XM_021467761.1
Accession Version	XM_021467761.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3a isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAGTCC 
ATCGTGCGCT CCAGTAAGCT GCTGAACTGG TGTCAGAGGC AGACGGAAGG CTACCGAGGT 
GTCAGCGTGT CTGATCTCAC CACGTCCTGG AAGAGTGGTT TGGCCCTGTG CGCGCTCATC 
CACAGATACA GACCAGACCT CATTGACTTT GAATCACTGG ACGAGAAAGA CGTGGAGAAG 
AACAACCAGC TGGCTTTCGA CGTGGCTGAA AGAGAGTTTG GGATTTCTCC CATCATGACC 
GGGAAGGAGA TGTCTGTTGT GGTGGAGCCA GATAAGCTCT CCATGGTGAT GTACCTCAGT 
CAGTTCTACG AGATGTTCAA GGACACGGTG CCACCTGGTG AAAACCAGAA TCTGAGCCCT 
GAGGAGAAGG CCGCACTGAT CGCCAGCACT AAATCACCCA TCTCATTCCT TAGTAAGCTA 
GGTCAAAGCA TCGCAATCTC CCGCAAAAGA AACCCCAAGG ATAAAAAAGA AAAGGAGCTG 
GATGGACTAG GGAAAAGACG AAAGACGAGC CAGGCTGGAC AATCGGAGGA CGAGGAGCTG 
CAGAGGGCTA ATCGAGACGA CAGGCCATCT ATAGCCACAG CTCTGGCAGA ACGTAAGATC 
GACTCCGCTG CAGCAGCCAA CAATAACAAC AAAGTGAAGT CGATGGCCAC GCAGCTGCTG 
GCCAAGTTTG AGGAGAACGC GCCGACACAG TCCACTGGGC TCAAACGACA GGGTTCCTTT 
CGAAAGGAGT TCCCTCAGAA CATCGGTGGC AGCGATGTGT GTTTCTTCTG CCGTAAACGG 
GTCTATGTGA TGGAGCGTCT CAGCGCTGAA GGAAAGTTTT TCCATCGCAG CTGCTTCAAG 
TGCGACTACT GCGGCACAAC CCTCCGCCTG TCCTCATATG CCTTCGATGT TGAAGACGGG 
AAGTTTTATT GTAAACCACA CTACTGCTAC CGACTCTCTG GTGTGGCACA GAGAAAACGG 
CCTGCACCTG CTGCTGCTCC AGCAAACGCC AAGGAGCCCC AGGTGTTGGC GGTATCCCCC 
AACACAGTGG ACGCCCCAGG TCAAGCCATA ACCACTCAGA CCCCTGCGGA GCGCCGGCCC 
TCAGAAACTG AGGTAAATGG TGTCACGGAG CCCAGCGTGG CCAAACGTCT GAAAGGGACT 
CCGGAAAGGA TCGAGCTGGA GAACTATCGT TTGTCAATGA TGAGGGAGGA GGAGCTGGAG 
GAGGTGCCGG AGGAGACATT GGCGGAGCAC AACCTCAGCA GTGTGCTGGA TAAAGCCACC 
GACATCGAGG AGGGATCCAG CAGCTCTGAG TCTGACATGG AGGAAGAGGA TGAAGATGCA 
GAGGCGGCCG GTCCGTCTGA TCTGGGTGGA GTTCCCTGGA AGGAGGCAGT AGAGCTCCAT 
GCTAAGTTAA AGGGAGAAAG CGACCCAGGA GCTGACGATG ATGGCCTTCA TGACGGTGAT 
GGAGAGATGG ACGAGGATGA AGAAGAGGAG GAAGATGAGG AAGATGAAGA TGAGGAGGAA 
GAAGAGGAAT CGAGCGAAGC GGGAGACTAT TTCCCCTGGG AGAGGGAGCT TCAGTCAGGC 
CTTTGGCTGG AAAATCTGGC AAATGAAGAG GATGTCAGTG TTTTTAAAGC TAGGAACCTG 
CAGATCCAGC AAGCCATTCA TCCAGTAGAC CCTCTGGACA TCCAGATGGA GAGCCATCAG 
GCTGAGGGGG TGTTGGAGAT GCCCTCGGTG TCACCCTTAG GCTCACAGCC CTCGCTCAAC 
ACCCAGCTCA CACAGCCCAC CTCCTCCACA GCCCCCTCCC ACAAATCCGT ACGTCACGAG 
GCTGTCAGAG CCTGGCTGGA CTCGATGTCT GGAGAGCCCT GTGAAGAGGA CGATGACCCT 
GAAGCTGAAG CAGGCAGTCC TGACTTTGAG CCAGGCACTG AAATAGATCA AGAGGACATT 
CCCTCAGATG CAGAGGCTGA AGCTCGTTCA CGGTGCATAG ATGAAGTGGT GACACTTCCA 
GTGGATAATG ATAAAACAGA AAGCCAGGGA CAGGTTTTCA ACACTGCAGA GAAAACGTCT 
GCAAATCCAA GGGAGTTGAT TGTGGACGTT GTGCTGTCTC CAATCCAAAA ACCTGCTTTA 
CCCATTGAGG AGGTGCATGA AGTTTCTCCT GTAGTACTAG TTAAATCTCC TGGTGCACGT 
TTCTTCCCTG AGCCCTATCT GCCTGATAAA GTCAAGCAGA ACATTCCCCC GCCTCAGAGT 
CCTGATGTTA AAACCCCTCA TTCTCCTGTA GCTCAAATCA TTGCGCTGTC GCCCATCTGT 
TCCCAGCCTG TTCCACAACC AGGCACAGCA TCTCCCAAAT CTCCAGTCCA ACCACAGCCT 
TGTGCCTGTT CTCCAACAGG CAATCCTTTG TCTCCGATTT GTACCCAATC GCAACCCTGC 
AATGAGCCAC CTTCACCCCT ATCCACAAGC TCCCCCGTCA GGACTCAACC AGTCCCAGCA 
GTAACTTCTA CTCCTTTAGC TAAACCTGCT TCCGAGAATA GGACTAATGA ACATTTGAAA 
GATTCAACTC CTGAGCTTAA GAAAACAGAC CTCATTGAGG AGTTCTGGTT GAAGAGTGCA 
GAGATTAGAA AAAGTTTAGG GCTCACTCCC CTGGAACGAA GCAAAACTGC GGTGGAGAAG 
AGCATTGTGA AAACTCCAAC ACCCGAATCA TCATCTCCTA AGTCTTATAC CCCAGAGGAC 
TTGTCTGAGG AACAGAAGCC TACATTTACG GGCCGTTCTA TCATACGCAG GATCAATATT 
ACCTTGGAGG GTCAGGTTAT ATCTCCGGTG GAACCTAAAA GTAATGGTTC AGAAAAGAAG 
GACTTAAGCA GCAGTTCAGG GTTGGGTCTA AACGGAAGTG TGACCACCAG TCAGACAGCT 
GCTAGCGACA GCTACAACAA CTCTGATTCT ACAATGCTTA CCCCTCCTTC CAGTCCACCA 
CCTCCTCCAC CCAGGGAGGA ACCTGCTTGT CTCCAAAACA AGAAGTCTCA GGTTTCCTGG 
GACAACCTCC TTGAAGGCAC AGAAGAACCC AAATCTGAGA CCATGCCCAT TAAGCCCAGG 
ACTCCAGTTA GTCCCCCTCA ACCAAAACAA AAACCAGTTA CAGCTCCAGT GCCAACCCCT 
AGAACCAATC CACCTGTTGT GATGAGGGTT AAGGAGCCCA ATAAACCACG TCGAGAAGAA 
GTGAGAAAGT CTTTTGTTGA GTGTGTAGAC GAGATACCAT TTGCTGATGA TGTTGAAGAC 
ACATACGATG ACCGCACTCC AGATGCAAGT GGTCTTGAAA AGTTTTACAC ACCGCCGACT 
AGCAAGGTCA ACCGGGATAA GCCGCCTCTC CACTTGGCAC TGGCTATGGA AAATGGCAAG 
CCCAATATCC CTGGAGTTTC CCGAACAGCA AAGGGTTCCC AGCATTTTTC TCCTGAAGCC 
AAGGAGATCG CAGAGGAGCG AATGAGGGCA CGGGAAAAGT CTGTGAAGAG CCAAGCTCTT 
AAAGATGCAA TGGCCAAGCA GCTTACAAAG ATGAAAGATT CTGAGGTTGC CAAGGGGGCT 
GTAGCAAAAG TAGCCTGGGA TATTCCTGAA ACAAAAGGAA AAAGCAAAAA GCAATCTAAA 
GCCCAGAAGG ACTCTGCAGT GAAGGCCTTG GAGTCCAAAA AGCAAGCAGA CACACTTCCA 
GACCGTTTCT TCTCTACACC ATCTAGTAAG GCCTTAGACA GCTCTGTCAC ATCTTCAGAG 
AGCTCCACAG GGGGCAAAAG CAAGAAACGC AGCTCACTGT TTTCTCCACG CAAAAACAAA 
AAAGAAAAGA AAGCCAAAAA TGAAAGACTG TCCAGCACTG AAGAAACGCC GCCCAAGCAC 
AAGTCCCTTT GGAAAGCAGT GTTCTCCGGC TACAAGAAGG ACAAAAAGAA GAAAGATGAC 
AAGTCATGCC CAAGTACACC CTCAAGCTCT ACAACTGGAG ACTCGGGGAA GAAGAAAGAT 
TCACCCCTGG ACAGGTCCTC AGATTTACGT CTGAGGAGAA ATCTAAGCTT TTCTGAGGAC 
TCTGATCTCT CCTGTGACGA TGTTCTGGAA AGATCCTCTC AGAAGTCCAA GGGTGATCGT 
CATGTGGACC TGTTTGACTT GGTGTCAGAT TTGAAAAAAG AAGCCATTAA AGAGGAGAAA 
GCAAAAGAGA AGGCTAAGGA TAGGGCAAAG GAAAGGGGAA GAGTTACTGA GAAGGGCAAG 
GAAAGGTCAA GAGAAAGGGA GAATGAAAAA AGAGTCAAAG AAAAGATGGT GAAAATGAAA 
GATGTGGAAC GGGAGAAAGG AAAAGAGGAA GAGTCTGTTT ATGTACCTCA TGCATTGGCG 
TTCAAGAGAT CATATGCCAC AAAGAAGACA TACACGGAGG AGGAACTGAA CGCCAAGCTG 
ACAAGAAGAG TACAGAAAGC TGCACGGAGG CAGGCCAAGC AGGAGGAGCT CAAGAGACTT 
CACAGAGCTC AGATGATTCA GAGGCAGCTG GAGCAGGTGG AAGAGAAACA GAGGCAGTTA 
GAGGAGAGAG GCGTCGCGGT GGAAAAGGCA CTGCGAGGCG AAGCAGGAAT GGGTAAAAAG 
GACGATCCGA AGCTGATGCA GGAATGGTTT AAACTGGTCC AGGAGAAGAA TGCCTTGGTA 
CGATATGAAT CCGAGCTGAT GATATTTGCT CGGGAGCTAG AGTTGGAGGA CAGACAGAGT 
CGACTGCAAC AGGAACTACG GGAACGCATG GCAATAGATG ATCATCTGAA GACAGAGGAG 
GAGCTGGCAG AGGAGAAGCA GATTCTGAAT GAGATGTTGG AGGTGGTGGA GCAGCGAGAC 
TCTCTGGTGG CCCTGCTGGA GGAACAGAGA CTCAGGGAGA AGGAAGAAGA TAAAGACCTG 
GAGGCGGTGA TGCTCTCCAA AGGATTCAAC CTCAACTGGG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323436.1
CDS	529..6993
Translation	MGDGGVNAVGEGVNQSHMLFDRFVQATTCKGTLKAFQELCDFLELKPNEYRVFYHKLKSKLNYWKAKALWAKLDKRASHKEYKKGRACANTKCLIIGAGPCGLRTAIELGFLGAKVVLLEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGIEIHVNVEFKGLIEPPEDQENERIGWRAEVHPRTHPVNELEFDVIIGADGRRNTLSGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILHDYADTEMLLSRANVDQKALLSYAREAADFSTNHQLPKLDFAINHYGQPDVAMFDFTCMYASENAALVRQRNGHKLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWAHGSSPLEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNISLHQVRPNQVRHLLDTGETRDLRVDLENVVNSSTPKLTRNESIVRSSKLLNWCQRQTEGYRGVSVSDLTTSWKSGLALCALIHRYRPDLIDFESLDEKDVEKNNQLAFDVAEREFGISPIMTGKEMSVVVEPDKLSMVMYLSQFYEMFKDTVPPGENQNLSPEEKAALIASTKSPISFLSKLGQSIAISRKRNPKDKKEKELDGLGKRRKTSQAGQSEDEELQRANRDDRPSIATALAERKIDSAAAANNNNKVKSMATQLLAKFEENAPTQSTGLKRQGSFRKEFPQNIGGSDVCFFCRKRVYVMERLSAEGKFFHRSCFKCDYCGTTLRLSSYAFDVEDGKFYCKPHYCYRLSGVAQRKRPAPAAAPANAKEPQVLAVSPNTVDAPGQAITTQTPAERRPSETEVNGVTEPSVAKRLKGTPERIELENYRLSMMREEELEEVPEETLAEHNLSSVLDKATDIEEGSSSSESDMEEEDEDAEAAGPSDLGGVPWKEAVELHAKLKGESDPGADDDGLHDGDGEMDEDEEEEEDEEDEDEEEEEESSEAGDYFPWERELQSGLWLENLANEEDVSVFKARNLQIQQAIHPVDPLDIQMESHQAEGVLEMPSVSPLGSQPSLNTQLTQPTSSTAPSHKSVRHEAVRAWLDSMSGEPCEEDDDPEAEAGSPDFEPGTEIDQEDIPSDAEAEARSRCIDEVVTLPVDNDKTESQGQVFNTAEKTSANPRELIVDVVLSPIQKPALPIEEVHEVSPVVLVKSPGARFFPEPYLPDKVKQNIPPPQSPDVKTPHSPVAQIIALSPICSQPVPQPGTASPKSPVQPQPCACSPTGNPLSPICTQSQPCNEPPSPLSTSSPVRTQPVPAVTSTPLAKPASENRTNEHLKDSTPELKKTDLIEEFWLKSAEIRKSLGLTPLERSKTAVEKSIVKTPTPESSSPKSYTPEDLSEEQKPTFTGRSIIRRINITLEGQVISPVEPKSNGSEKKDLSSSSGLGLNGSVTTSQTAASDSYNNSDSTMLTPPSSPPPPPPREEPACLQNKKSQVSWDNLLEGTEEPKSETMPIKPRTPVSPPQPKQKPVTAPVPTPRTNPPVVMRVKEPNKPRREEVRKSFVECVDEIPFADDVEDTYDDRTPDASGLEKFYTPPTSKVNRDKPPLHLALAMENGKPNIPGVSRTAKGSQHFSPEAKEIAEERMRAREKSVKSQALKDAMAKQLTKMKDSEVAKGAVAKVAWDIPETKGKSKKQSKAQKDSAVKALESKKQADTLPDRFFSTPSSKALDSSVTSSESSTGGKSKKRSSLFSPRKNKKEKKAKNERLSSTEETPPKHKSLWKAVFSGYKKDKKKKDDKSCPSTPSSSTTGDSGKKKDSPLDRSSDLRLRRNLSFSEDSDLSCDDVLERSSQKSKGDRHVDLFDLVSDLKKEAIKEEKAKEKAKDRAKERGRVTEKGKERSRERENEKRVKEKMVKMKDVEREKGKEEESVYVPHALAFKRSYATKKTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQMIQRQLEQVEEKQRQLEERGVAVEKALRGEAGMGKKDDPKLMQEWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAIDDHLKTEEELAEEKQILNEMLEVVEQRDSLVALLEEQRLREKEEDKDLEAVMLSKGFNLNWA

Target ORF information:

RefSeq Version	XM_021467761.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467761.1

ORF Insert Sequence:

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAGTCC 
ATCGTGCGCT CCAGTAAGCT GCTGAACTGG TGTCAGAGGC AGACGGAAGG CTACCGAGGT 
GTCAGCGTGT CTGATCTCAC CACGTCCTGG AAGAGTGGTT TGGCCCTGTG CGCGCTCATC 
CACAGATACA GACCAGACCT CATTGACTTT GAATCACTGG ACGAGAAAGA CGTGGAGAAG 
AACAACCAGC TGGCTTTCGA CGTGGCTGAA AGAGAGTTTG GGATTTCTCC CATCATGACC 
GGGAAGGAGA TGTCTGTTGT GGTGGAGCCA GATAAGCTCT CCATGGTGAT GTACCTCAGT 
CAGTTCTACG AGATGTTCAA GGACACGGTG CCACCTGGTG AAAACCAGAA TCTGAGCCCT 
GAGGAGAAGG CCGCACTGAT CGCCAGCACT AAATCACCCA TCTCATTCCT TAGTAAGCTA 
GGTCAAAGCA TCGCAATCTC CCGCAAAAGA AACCCCAAGG ATAAAAAAGA AAAGGAGCTG 
GATGGACTAG GGAAAAGACG AAAGACGAGC CAGGCTGGAC AATCGGAGGA CGAGGAGCTG 
CAGAGGGCTA ATCGAGACGA CAGGCCATCT ATAGCCACAG CTCTGGCAGA ACGTAAGATC 
GACTCCGCTG CAGCAGCCAA CAATAACAAC AAAGTGAAGT CGATGGCCAC GCAGCTGCTG 
GCCAAGTTTG AGGAGAACGC GCCGACACAG TCCACTGGGC TCAAACGACA GGGTTCCTTT 
CGAAAGGAGT TCCCTCAGAA CATCGGTGGC AGCGATGTGT GTTTCTTCTG CCGTAAACGG 
GTCTATGTGA TGGAGCGTCT CAGCGCTGAA GGAAAGTTTT TCCATCGCAG CTGCTTCAAG 
TGCGACTACT GCGGCACAAC CCTCCGCCTG TCCTCATATG CCTTCGATGT TGAAGACGGG 
AAGTTTTATT GTAAACCACA CTACTGCTAC CGACTCTCTG GTGTGGCACA GAGAAAACGG 
CCTGCACCTG CTGCTGCTCC AGCAAACGCC AAGGAGCCCC AGGTGTTGGC GGTATCCCCC 
AACACAGTGG ACGCCCCAGG TCAAGCCATA ACCACTCAGA CCCCTGCGGA GCGCCGGCCC 
TCAGAAACTG AGGTAAATGG TGTCACGGAG CCCAGCGTGG CCAAACGTCT GAAAGGGACT 
CCGGAAAGGA TCGAGCTGGA GAACTATCGT TTGTCAATGA TGAGGGAGGA GGAGCTGGAG 
GAGGTGCCGG AGGAGACATT GGCGGAGCAC AACCTCAGCA GTGTGCTGGA TAAAGCCACC 
GACATCGAGG AGGGATCCAG CAGCTCTGAG TCTGACATGG AGGAAGAGGA TGAAGATGCA 
GAGGCGGCCG GTCCGTCTGA TCTGGGTGGA GTTCCCTGGA AGGAGGCAGT AGAGCTCCAT 
GCTAAGTTAA AGGGAGAAAG CGACCCAGGA GCTGACGATG ATGGCCTTCA TGACGGTGAT 
GGAGAGATGG ACGAGGATGA AGAAGAGGAG GAAGATGAGG AAGATGAAGA TGAGGAGGAA 
GAAGAGGAAT CGAGCGAAGC GGGAGACTAT TTCCCCTGGG AGAGGGAGCT TCAGTCAGGC 
CTTTGGCTGG AAAATCTGGC AAATGAAGAG GATGTCAGTG TTTTTAAAGC TAGGAACCTG 
CAGATCCAGC AAGCCATTCA TCCAGTAGAC CCTCTGGACA TCCAGATGGA GAGCCATCAG 
GCTGAGGGGG TGTTGGAGAT GCCCTCGGTG TCACCCTTAG GCTCACAGCC CTCGCTCAAC 
ACCCAGCTCA CACAGCCCAC CTCCTCCACA GCCCCCTCCC ACAAATCCGT ACGTCACGAG 
GCTGTCAGAG CCTGGCTGGA CTCGATGTCT GGAGAGCCCT GTGAAGAGGA CGATGACCCT 
GAAGCTGAAG CAGGCAGTCC TGACTTTGAG CCAGGCACTG AAATAGATCA AGAGGACATT 
CCCTCAGATG CAGAGGCTGA AGCTCGTTCA CGGTGCATAG ATGAAGTGGT GACACTTCCA 
GTGGATAATG ATAAAACAGA AAGCCAGGGA CAGGTTTTCA ACACTGCAGA GAAAACGTCT 
GCAAATCCAA GGGAGTTGAT TGTGGACGTT GTGCTGTCTC CAATCCAAAA ACCTGCTTTA 
CCCATTGAGG AGGTGCATGA AGTTTCTCCT GTAGTACTAG TTAAATCTCC TGGTGCACGT 
TTCTTCCCTG AGCCCTATCT GCCTGATAAA GTCAAGCAGA ACATTCCCCC GCCTCAGAGT 
CCTGATGTTA AAACCCCTCA TTCTCCTGTA GCTCAAATCA TTGCGCTGTC GCCCATCTGT 
TCCCAGCCTG TTCCACAACC AGGCACAGCA TCTCCCAAAT CTCCAGTCCA ACCACAGCCT 
TGTGCCTGTT CTCCAACAGG CAATCCTTTG TCTCCGATTT GTACCCAATC GCAACCCTGC 
AATGAGCCAC CTTCACCCCT ATCCACAAGC TCCCCCGTCA GGACTCAACC AGTCCCAGCA 
GTAACTTCTA CTCCTTTAGC TAAACCTGCT TCCGAGAATA GGACTAATGA ACATTTGAAA 
GATTCAACTC CTGAGCTTAA GAAAACAGAC CTCATTGAGG AGTTCTGGTT GAAGAGTGCA 
GAGATTAGAA AAAGTTTAGG GCTCACTCCC CTGGAACGAA GCAAAACTGC GGTGGAGAAG 
AGCATTGTGA AAACTCCAAC ACCCGAATCA TCATCTCCTA AGTCTTATAC CCCAGAGGAC 
TTGTCTGAGG AACAGAAGCC TACATTTACG GGCCGTTCTA TCATACGCAG GATCAATATT 
ACCTTGGAGG GTCAGGTTAT ATCTCCGGTG GAACCTAAAA GTAATGGTTC AGAAAAGAAG 
GACTTAAGCA GCAGTTCAGG GTTGGGTCTA AACGGAAGTG TGACCACCAG TCAGACAGCT 
GCTAGCGACA GCTACAACAA CTCTGATTCT ACAATGCTTA CCCCTCCTTC CAGTCCACCA 
CCTCCTCCAC CCAGGGAGGA ACCTGCTTGT CTCCAAAACA AGAAGTCTCA GGTTTCCTGG 
GACAACCTCC TTGAAGGCAC AGAAGAACCC AAATCTGAGA CCATGCCCAT TAAGCCCAGG 
ACTCCAGTTA GTCCCCCTCA ACCAAAACAA AAACCAGTTA CAGCTCCAGT GCCAACCCCT 
AGAACCAATC CACCTGTTGT GATGAGGGTT AAGGAGCCCA ATAAACCACG TCGAGAAGAA 
GTGAGAAAGT CTTTTGTTGA GTGTGTAGAC GAGATACCAT TTGCTGATGA TGTTGAAGAC 
ACATACGATG ACCGCACTCC AGATGCAAGT GGTCTTGAAA AGTTTTACAC ACCGCCGACT 
AGCAAGGTCA ACCGGGATAA GCCGCCTCTC CACTTGGCAC TGGCTATGGA AAATGGCAAG 
CCCAATATCC CTGGAGTTTC CCGAACAGCA AAGGGTTCCC AGCATTTTTC TCCTGAAGCC 
AAGGAGATCG CAGAGGAGCG AATGAGGGCA CGGGAAAAGT CTGTGAAGAG CCAAGCTCTT 
AAAGATGCAA TGGCCAAGCA GCTTACAAAG ATGAAAGATT CTGAGGTTGC CAAGGGGGCT 
GTAGCAAAAG TAGCCTGGGA TATTCCTGAA ACAAAAGGAA AAAGCAAAAA GCAATCTAAA 
GCCCAGAAGG ACTCTGCAGT GAAGGCCTTG GAGTCCAAAA AGCAAGCAGA CACACTTCCA 
GACCGTTTCT TCTCTACACC ATCTAGTAAG GCCTTAGACA GCTCTGTCAC ATCTTCAGAG 
AGCTCCACAG GGGGCAAAAG CAAGAAACGC AGCTCACTGT TTTCTCCACG CAAAAACAAA 
AAAGAAAAGA AAGCCAAAAA TGAAAGACTG TCCAGCACTG AAGAAACGCC GCCCAAGCAC 
AAGTCCCTTT GGAAAGCAGT GTTCTCCGGC TACAAGAAGG ACAAAAAGAA GAAAGATGAC 
AAGTCATGCC CAAGTACACC CTCAAGCTCT ACAACTGGAG ACTCGGGGAA GAAGAAAGAT 
TCACCCCTGG ACAGGTCCTC AGATTTACGT CTGAGGAGAA ATCTAAGCTT TTCTGAGGAC 
TCTGATCTCT CCTGTGACGA TGTTCTGGAA AGATCCTCTC AGAAGTCCAA GGGTGATCGT 
CATGTGGACC TGTTTGACTT GGTGTCAGAT TTGAAAAAAG AAGCCATTAA AGAGGAGAAA 
GCAAAAGAGA AGGCTAAGGA TAGGGCAAAG GAAAGGGGAA GAGTTACTGA GAAGGGCAAG 
GAAAGGTCAA GAGAAAGGGA GAATGAAAAA AGAGTCAAAG AAAAGATGGT GAAAATGAAA 
GATGTGGAAC GGGAGAAAGG AAAAGAGGAA GAGTCTGTTT ATGTACCTCA TGCATTGGCG 
TTCAAGAGAT CATATGCCAC AAAGAAGACA TACACGGAGG AGGAACTGAA CGCCAAGCTG 
ACAAGAAGAG TACAGAAAGC TGCACGGAGG CAGGCCAAGC AGGAGGAGCT CAAGAGACTT 
CACAGAGCTC AGATGATTCA GAGGCAGCTG GAGCAGGTGG AAGAGAAACA GAGGCAGTTA 
GAGGAGAGAG GCGTCGCGGT GGAAAAGGCA CTGCGAGGCG AAGCAGGAAT GGGTAAAAAG 
GACGATCCGA AGCTGATGCA GGAATGGTTT AAACTGGTCC AGGAGAAGAA TGCCTTGGTA 
CGATATGAAT CCGAGCTGAT GATATTTGCT CGGGAGCTAG AGTTGGAGGA CAGACAGAGT 
CGACTGCAAC AGGAACTACG GGAACGCATG GCAATAGATG ATCATCTGAA GACAGAGGAG 
GAGCTGGCAG AGGAGAAGCA GATTCTGAAT GAGATGTTGG AGGTGGTGGA GCAGCGAGAC 
TCTCTGGTGG CCCTGCTGGA GGAACAGAGA CTCAGGGAGA AGGAAGAAGA TAAAGACCTG 
GAGGCGGTGA TGCTCTCCAA AGGATTCAAC CTCAACTGGG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3A ( XM_021473239.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021328914.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X6, mRNA.

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CloneID	ODa145983
Clone ID Related Accession (Same CDS sequence)	XM_021473239.1 , XM_021467762.1
Accession Version	XM_021473239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3a isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395007.1) and transcript sequence (GFIL01026059.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1201 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAGTCC 
ATCGTGCGCT CCAGTAAGCT GCTGAACTGG TGTCAGAGGC AGACGGAAGG CTACCGAGGT 
GTCAGCGTGT CTGATCTCAC CACGTCCTGG AAGAGTGGTT TGGCCCTGTG CGCGCTCATC 
CACAGATACA GACCAGACCT CATTGACTTT GAATCACTGG ACGAGAAAGA CGTGGAGAAG 
AACAACCAGC TGGCTTTCGA CGTGGCTGAA AGAGAGTTTG GGATTTCTCC CATCATGACC 
GGGAAGGAGA TGTCTGTTGT GGTGGAGCCA GATAAGCTCT CCATGGTGAT GTACCTCAGT 
CAGTTCTACG AGATGTTCAA GGACACGGTG CCACCTGGTG AAAACCAGAA TCTGAGCCCT 
GAGGAGAAGG CCGCACTGAT CGCCAGCACT AAATCACCCA TCTCATTCCT TAGTAAGCTA 
GGTCAAAGCA TCGCAATCTC CCGCAAAAGA AACCCCAAGG ATAAAAAAGA AAAGGAGCTG 
GATGGACTAG GGAAAAGACG AAAGACGAGC CAGGCTGGAC AATCGGAGGA CGAGGAGCTG 
CAGAGGGCTA ATCGAGACGA CAGGCCATCT ATAGCCACAG CTCTGGCAGA ACGTAAGATC 
GACTCCGCTG CAGCAGCCAA CAATAACAAC AAAGTGAAGT CGATGGCCAC GCAGCTGCTG 
GCCAAGTTTG AGGAGAACGC GCCGACACAG TCCACTGGGC TCAAACGACA GGGTTCCTTT 
CGAAAGGAGT TCCCTCAGAA CATCGGTGGC AGCGATGTGT GTTTCTTCTG CCGTAAACGG 
GTCTATGTGA TGGAGCGTCT CAGCGCTGAA GGAAAGTTTT TCCATCGCAG CTGCTTCAAG 
TGCGACTACT GCGGCACAAC CCTCCGCCTG TCCTCATATG CCTTCGATGT TGAAGACGGG 
AAGTTTTATT GTAAACCACA CTACTGCTAC CGACTCTCTG GTGTGGCACA GAGAAAACGG 
CCTGCACCTG CTGCTGCTCC AGCAAACGCC AAGGAGCCCC AGGTGTTGGC GGTATCCCCC 
AACACAGTGG ACGCCCCAGG TCAAGCCATA ACCACTCAGA CCCCTGCGGA GCGCCGGCCC 
TCAGAAACTG AGGTAAATGG TGTCACGGAG CCCAGCGTGG CCAAACGTCT GAAAGGGACT 
CCGGAAAGGA TCGAGCTGGA GAACTATCGT TTGTCAATGA TGAGGGAGGA GGAGCTGGAG 
GAGGTGCCGG AGGAGACATT GGCGGAGCAC AACCTCAGCA GTGTGCTGGA TAAAGCCACC 
GACATCGAGG AGGGATCCAG CAGCTCTGAG TCTGACATGG AGGAAGAGGA TGAAGATGCA 
GAGGCGGCCG GTCCGTCTGA TCTGGGTGGA GTTCCCTGGA AGGAGGCAGT AGAGCTCCAT 
GCTAAGTTAA AGGGAGAAAG CGACCCAGGA GCTGACGATG ATGGCCTTCA TGACGGTGAT 
GGAGAGATGG ACGAGGATGA AGAAGAGGAG GAAGATGAGG AAGATGAAGA TGAGGAGGAA 
GAAGAGGAAT CGAGCGAAGC TAGGAACCTG CAGATCCAGC AAGCCATTCA TCCAGTAGAC 
CCTCTGGACA TCCAGATGGA GAGCCATCAG GCTGAGGGGG TGTTGGAGAT GCCCTCGGTG 
TCACCCTTAG GCTCACAGCC CTCGCTCAAC ACCCAGCTCA CACAGCCCAC CTCCTCCACA 
GCCCCCTCCC ACAAATCCGT ACGTCACGAG GCTGTCAGAG CCTGGCTGGA CTCGATGTCT 
GGAGAGCCCT GTGAAGAGGA CGATGACCCT GAAGCTGAAG CAGGCAGTCC TGACTTTGAG 
CCAGGCACTG AAATAGATCA AGAGGACATT CCCTCAGATG CAGAGGCTGA AGCTCGTTCA 
CGGTGCATAG ATGAAGTGGT GACACTTCCA GTGGATAATG ATAAAACAGA AAGCCAGGGA 
CAGGTTTTCA ACACTGCAGA GAAAACGTCT GCAAATCCAA GGGAGTTGAT TGTGGACGTT 
GTGCTGTCTC CAATCCAAAA ACCTGCTTTA CCCATTGAGG AGGTGCATGA AGTTTCTCCT 
GTAGTACTAG TTAAATCTCC TGGTGCACGT TTCTTCCCTG AGCCCTATCT GCCTGATAAA 
GTCAAGCAGA ACATTCCCCC GCCTCAGAGT CCTGATGTTA AAACCCCTCA TTCTCCTGTA 
GCTCAAATCA TTGCGCTGTC GCCCATCTGT TCCCAGCCTG TTCCACAACC AGGCACAGCA 
TCTCCCAAAT CTCCAGTCCA ACCACAGCCT TGTGCCTGTT CTCCAACAGG CAATCCTTTG 
TCTCCGATTT GTACCCAATC GCAACCCTGC AATGAGCCAC CTTCACCCCT ATCCACAAGC 
TCCCCCGTCA GGACTCAACC AGTCCCAGCA GTAACTTCTA CTCCTTTAGC TAAACCTGCT 
TCCGAGAATA GGACTAATGA ACATTTGAAA GATTCAACTC CTGAGCTTAA GAAAACAGAC 
CTCATTGAGG AGTTCTGGTT GAAGAGTGCA GAGATTAGAA AAAGTTTAGG GCTCACTCCC 
CTGGAACGAA GCAAAACTGC GGTGGAGAAG AGCATTGTGA AAACTCCAAC ACCCGAATCA 
TCATCTCCTA AGTCTTATAC CCCAGAGGAC TTGTCTGAGG AACAGAAGCC TACATTTACG 
GGCCGTTCTA TCATACGCAG GATCAATATT ACCTTGGAGG GTCAGGTTAT ATCTCCGGTG 
GAACCTAAAA GTAATGGTTC AGAAAAGAAG GACTTAAGCA GCAGTTCAGG GTTGGGTCTA 
AACGGAAGTG TGACCACCAG TCAGACAGCT GCTAGCGACA GCTACAACAA CTCTGATTCT 
ACAATGCTTA CCCCTCCTTC CAGTCCACCA CCTCCTCCAC CCAGGGAGGA ACCTGCTTGT 
CTCCAAAACA AGAAGTCTCA GGTTTCCTGG GACAACCTCC TTGAAGGCAC AGAAGAACCC 
AAATCTGAGA CCATGCCCAT TAAGCCCAGG ACTCCAGTTA GTCCCCCTCA ACCAAAACAA 
AAACCAGTTA CAGCTCCAGT GCCAACCCCT AGAACCAATC CACCTGTTGT GATGAGGGTT 
AAGGAGCCCA ATAAACCACG TCGAGAAGAA GTGAGAAAGT CTTTTGTTGA GTGTGTAGAC 
GAGATACCAT TTGCTGATGA TGTTGAAGAC ACATACGATG ACCGCACTCC AGATGCAAGT 
GGTCTTGAAA AGTTTTACAC ACCGCCGACT AGCAAGGTCA ACCGGGATAA GCCGCCTCTC 
CACTTGGCAC TGGCTATGGA AAATGGCAAG CCCAATATCC CTGGAGTTTC CCGAACAGCA 
AAGGGTTCCC AGCATTTTTC TCCTGAAGCC AAGGAGATCG CAGAGGAGCG AATGAGGGCA 
CGGGAAAAGT CTGTGAAGAG CCAAGCTCTT AAAGATGCAA TGGCCAAGCA GCTTACAAAG 
ATGAAAGATT CTGAGGTTGC CAAGGGGGCT GTAGCAAAAG TAGCCTGGGA TATTCCTGAA 
ACAAAAGGAA AAAGCAAAAA GCAATCTAAA GCCCAGAAGG ACTCTGCAGT GAAGGCCTTG 
GAGTCCAAAA AGCAAGCAGA CACACTTCCA GACCGTTTCT TCTCTACACC ATCTAGTAAG 
GCCTTAGACA GCTCTGTCAC ATCTTCAGAG AGCTCCACAG GGGGCAAAAG CAAGAAACGC 
AGCTCACTGT TTTCTCCACG CAAAAACAAA AAAGAAAAGA AAGCCAAAAA TGAAAGACTG 
TCCAGCACTG AAGAAACGCC GCCCAAGCAC AAGTCCCTTT GGAAAGCAGT GTTCTCCGGC 
TACAAGAAGG ACAAAAAGAA GAAAGATGAC AAGTCATGCC CAAGTACACC CTCAAGCTCT 
ACAACTGGAG ACTCGGGGAA GAAGAAAGAT TCACCCCTGG ACAGGTCCTC AGATTTACGT 
CTGAGGAGAA ATCTAAGCTT TTCTGAGGAC TCTGATCTCT CCTGTGACGA TGTTCTGGAA 
AGATCCTCTC AGAAGTCCAA GGGTGATCGT CATGTGGACC TGTTTGACTT GGTGTCAGAT 
TTGAAAAAAG AAGCCATTAA AGAGGAGAAA GCAAAAGAGA AGGCTAAGGA TAGGGCAAAG 
GAAAGGGGAA GAGTTACTGA GAAGGGCAAG GAAAGGTCAA GAGAAAGGGA GAATGAAAAA 
AGAGTCAAAG AAAAGATGGT GAAAATGAAA GATGTGGAAC GGGAGAAAGG AAAAGAGGAA 
GAGTCTGTTT ATGTACCTCA TGCATTGGCG TTCAAGAGAT CATATGCCAC AAAGAAGACA 
TACACGGAGG AGGAACTGAA CGCCAAGCTG ACAAGAAGAG TACAGAAAGC TGCACGGAGG 
CAGGCCAAGC AGGAGGAGCT CAAGAGACTT CACAGAGCTC AGATGATTCA GAGGCAGCTG 
GAGCAGGTGG AAGAGAAACA GAGGCAGTTA GAGGAGAGAG GCGTCGCGGT GGAAAAGGCA 
CTGCGAGGCG AAGCAGGAAT GGGTAAAAAG GACGATCCGA AGCTGATGCA GGAATGGTTT 
AAACTGGTCC AGGAGAAGAA TGCCTTGGTA CGATATGAAT CCGAGCTGAT GATATTTGCT 
CGGGAGCTAG AGTTGGAGGA CAGACAGAGT CGACTGCAAC AGGAACTACG GGAACGCATG 
GCAATAGATG ATCATCTGAA GACAGAGGAG GAGCTGGCAG AGGAGAAGCA GATTCTGAAT 
GAGATGTTGG AGGTGGTGGA GCAGCGAGAC TCTCTGGTGG CCCTGCTGGA GGAACAGAGA 
CTCAGGGAGA AGGAAGAAGA TAAAGACCTG GAGGCGGTGA TGCTCTCCAA AGGATTCAAC 
CTCAACTGGG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328914.1
CDS	349..6723
Translation	MGDGGVNAVGEGVNQSHMLFDRFVQATTCKGTLKAFQELCDFLELKPNEYRVFYHKLKSKLNYWKAKALWAKLDKRASHKEYKKGRACANTKCLIIGAGPCGLRTAIELGFLGAKVVLLEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGIEIHVNVEFKGLIEPPEDQENERIGWRAEVHPRTHPVNELEFDVIIGADGRRNTLSGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILHDYADTEMLLSRANVDQKALLSYAREAADFSTNHQLPKLDFAINHYGQPDVAMFDFTCMYASENAALVRQRNGHKLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWAHGSSPLEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNISLHQVRPNQVRHLLDTGETRDLRVDLENVVNSSTPKLTRNESIVRSSKLLNWCQRQTEGYRGVSVSDLTTSWKSGLALCALIHRYRPDLIDFESLDEKDVEKNNQLAFDVAEREFGISPIMTGKEMSVVVEPDKLSMVMYLSQFYEMFKDTVPPGENQNLSPEEKAALIASTKSPISFLSKLGQSIAISRKRNPKDKKEKELDGLGKRRKTSQAGQSEDEELQRANRDDRPSIATALAERKIDSAAAANNNNKVKSMATQLLAKFEENAPTQSTGLKRQGSFRKEFPQNIGGSDVCFFCRKRVYVMERLSAEGKFFHRSCFKCDYCGTTLRLSSYAFDVEDGKFYCKPHYCYRLSGVAQRKRPAPAAAPANAKEPQVLAVSPNTVDAPGQAITTQTPAERRPSETEVNGVTEPSVAKRLKGTPERIELENYRLSMMREEELEEVPEETLAEHNLSSVLDKATDIEEGSSSSESDMEEEDEDAEAAGPSDLGGVPWKEAVELHAKLKGESDPGADDDGLHDGDGEMDEDEEEEEDEEDEDEEEEEESSEARNLQIQQAIHPVDPLDIQMESHQAEGVLEMPSVSPLGSQPSLNTQLTQPTSSTAPSHKSVRHEAVRAWLDSMSGEPCEEDDDPEAEAGSPDFEPGTEIDQEDIPSDAEAEARSRCIDEVVTLPVDNDKTESQGQVFNTAEKTSANPRELIVDVVLSPIQKPALPIEEVHEVSPVVLVKSPGARFFPEPYLPDKVKQNIPPPQSPDVKTPHSPVAQIIALSPICSQPVPQPGTASPKSPVQPQPCACSPTGNPLSPICTQSQPCNEPPSPLSTSSPVRTQPVPAVTSTPLAKPASENRTNEHLKDSTPELKKTDLIEEFWLKSAEIRKSLGLTPLERSKTAVEKSIVKTPTPESSSPKSYTPEDLSEEQKPTFTGRSIIRRINITLEGQVISPVEPKSNGSEKKDLSSSSGLGLNGSVTTSQTAASDSYNNSDSTMLTPPSSPPPPPPREEPACLQNKKSQVSWDNLLEGTEEPKSETMPIKPRTPVSPPQPKQKPVTAPVPTPRTNPPVVMRVKEPNKPRREEVRKSFVECVDEIPFADDVEDTYDDRTPDASGLEKFYTPPTSKVNRDKPPLHLALAMENGKPNIPGVSRTAKGSQHFSPEAKEIAEERMRAREKSVKSQALKDAMAKQLTKMKDSEVAKGAVAKVAWDIPETKGKSKKQSKAQKDSAVKALESKKQADTLPDRFFSTPSSKALDSSVTSSESSTGGKSKKRSSLFSPRKNKKEKKAKNERLSSTEETPPKHKSLWKAVFSGYKKDKKKKDDKSCPSTPSSSTTGDSGKKKDSPLDRSSDLRLRRNLSFSEDSDLSCDDVLERSSQKSKGDRHVDLFDLVSDLKKEAIKEEKAKEKAKDRAKERGRVTEKGKERSRERENEKRVKEKMVKMKDVEREKGKEEESVYVPHALAFKRSYATKKTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQMIQRQLEQVEEKQRQLEERGVAVEKALRGEAGMGKKDDPKLMQEWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAIDDHLKTEEELAEEKQILNEMLEVVEQRDSLVALLEEQRLREKEEDKDLEAVMLSKGFNLNWA

Target ORF information:

RefSeq Version	XM_021473239.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473239.1

ORF Insert Sequence:

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAGTCC 
ATCGTGCGCT CCAGTAAGCT GCTGAACTGG TGTCAGAGGC AGACGGAAGG CTACCGAGGT 
GTCAGCGTGT CTGATCTCAC CACGTCCTGG AAGAGTGGTT TGGCCCTGTG CGCGCTCATC 
CACAGATACA GACCAGACCT CATTGACTTT GAATCACTGG ACGAGAAAGA CGTGGAGAAG 
AACAACCAGC TGGCTTTCGA CGTGGCTGAA AGAGAGTTTG GGATTTCTCC CATCATGACC 
GGGAAGGAGA TGTCTGTTGT GGTGGAGCCA GATAAGCTCT CCATGGTGAT GTACCTCAGT 
CAGTTCTACG AGATGTTCAA GGACACGGTG CCACCTGGTG AAAACCAGAA TCTGAGCCCT 
GAGGAGAAGG CCGCACTGAT CGCCAGCACT AAATCACCCA TCTCATTCCT TAGTAAGCTA 
GGTCAAAGCA TCGCAATCTC CCGCAAAAGA AACCCCAAGG ATAAAAAAGA AAAGGAGCTG 
GATGGACTAG GGAAAAGACG AAAGACGAGC CAGGCTGGAC AATCGGAGGA CGAGGAGCTG 
CAGAGGGCTA ATCGAGACGA CAGGCCATCT ATAGCCACAG CTCTGGCAGA ACGTAAGATC 
GACTCCGCTG CAGCAGCCAA CAATAACAAC AAAGTGAAGT CGATGGCCAC GCAGCTGCTG 
GCCAAGTTTG AGGAGAACGC GCCGACACAG TCCACTGGGC TCAAACGACA GGGTTCCTTT 
CGAAAGGAGT TCCCTCAGAA CATCGGTGGC AGCGATGTGT GTTTCTTCTG CCGTAAACGG 
GTCTATGTGA TGGAGCGTCT CAGCGCTGAA GGAAAGTTTT TCCATCGCAG CTGCTTCAAG 
TGCGACTACT GCGGCACAAC CCTCCGCCTG TCCTCATATG CCTTCGATGT TGAAGACGGG 
AAGTTTTATT GTAAACCACA CTACTGCTAC CGACTCTCTG GTGTGGCACA GAGAAAACGG 
CCTGCACCTG CTGCTGCTCC AGCAAACGCC AAGGAGCCCC AGGTGTTGGC GGTATCCCCC 
AACACAGTGG ACGCCCCAGG TCAAGCCATA ACCACTCAGA CCCCTGCGGA GCGCCGGCCC 
TCAGAAACTG AGGTAAATGG TGTCACGGAG CCCAGCGTGG CCAAACGTCT GAAAGGGACT 
CCGGAAAGGA TCGAGCTGGA GAACTATCGT TTGTCAATGA TGAGGGAGGA GGAGCTGGAG 
GAGGTGCCGG AGGAGACATT GGCGGAGCAC AACCTCAGCA GTGTGCTGGA TAAAGCCACC 
GACATCGAGG AGGGATCCAG CAGCTCTGAG TCTGACATGG AGGAAGAGGA TGAAGATGCA 
GAGGCGGCCG GTCCGTCTGA TCTGGGTGGA GTTCCCTGGA AGGAGGCAGT AGAGCTCCAT 
GCTAAGTTAA AGGGAGAAAG CGACCCAGGA GCTGACGATG ATGGCCTTCA TGACGGTGAT 
GGAGAGATGG ACGAGGATGA AGAAGAGGAG GAAGATGAGG AAGATGAAGA TGAGGAGGAA 
GAAGAGGAAT CGAGCGAAGC TAGGAACCTG CAGATCCAGC AAGCCATTCA TCCAGTAGAC 
CCTCTGGACA TCCAGATGGA GAGCCATCAG GCTGAGGGGG TGTTGGAGAT GCCCTCGGTG 
TCACCCTTAG GCTCACAGCC CTCGCTCAAC ACCCAGCTCA CACAGCCCAC CTCCTCCACA 
GCCCCCTCCC ACAAATCCGT ACGTCACGAG GCTGTCAGAG CCTGGCTGGA CTCGATGTCT 
GGAGAGCCCT GTGAAGAGGA CGATGACCCT GAAGCTGAAG CAGGCAGTCC TGACTTTGAG 
CCAGGCACTG AAATAGATCA AGAGGACATT CCCTCAGATG CAGAGGCTGA AGCTCGTTCA 
CGGTGCATAG ATGAAGTGGT GACACTTCCA GTGGATAATG ATAAAACAGA AAGCCAGGGA 
CAGGTTTTCA ACACTGCAGA GAAAACGTCT GCAAATCCAA GGGAGTTGAT TGTGGACGTT 
GTGCTGTCTC CAATCCAAAA ACCTGCTTTA CCCATTGAGG AGGTGCATGA AGTTTCTCCT 
GTAGTACTAG TTAAATCTCC TGGTGCACGT TTCTTCCCTG AGCCCTATCT GCCTGATAAA 
GTCAAGCAGA ACATTCCCCC GCCTCAGAGT CCTGATGTTA AAACCCCTCA TTCTCCTGTA 
GCTCAAATCA TTGCGCTGTC GCCCATCTGT TCCCAGCCTG TTCCACAACC AGGCACAGCA 
TCTCCCAAAT CTCCAGTCCA ACCACAGCCT TGTGCCTGTT CTCCAACAGG CAATCCTTTG 
TCTCCGATTT GTACCCAATC GCAACCCTGC AATGAGCCAC CTTCACCCCT ATCCACAAGC 
TCCCCCGTCA GGACTCAACC AGTCCCAGCA GTAACTTCTA CTCCTTTAGC TAAACCTGCT 
TCCGAGAATA GGACTAATGA ACATTTGAAA GATTCAACTC CTGAGCTTAA GAAAACAGAC 
CTCATTGAGG AGTTCTGGTT GAAGAGTGCA GAGATTAGAA AAAGTTTAGG GCTCACTCCC 
CTGGAACGAA GCAAAACTGC GGTGGAGAAG AGCATTGTGA AAACTCCAAC ACCCGAATCA 
TCATCTCCTA AGTCTTATAC CCCAGAGGAC TTGTCTGAGG AACAGAAGCC TACATTTACG 
GGCCGTTCTA TCATACGCAG GATCAATATT ACCTTGGAGG GTCAGGTTAT ATCTCCGGTG 
GAACCTAAAA GTAATGGTTC AGAAAAGAAG GACTTAAGCA GCAGTTCAGG GTTGGGTCTA 
AACGGAAGTG TGACCACCAG TCAGACAGCT GCTAGCGACA GCTACAACAA CTCTGATTCT 
ACAATGCTTA CCCCTCCTTC CAGTCCACCA CCTCCTCCAC CCAGGGAGGA ACCTGCTTGT 
CTCCAAAACA AGAAGTCTCA GGTTTCCTGG GACAACCTCC TTGAAGGCAC AGAAGAACCC 
AAATCTGAGA CCATGCCCAT TAAGCCCAGG ACTCCAGTTA GTCCCCCTCA ACCAAAACAA 
AAACCAGTTA CAGCTCCAGT GCCAACCCCT AGAACCAATC CACCTGTTGT GATGAGGGTT 
AAGGAGCCCA ATAAACCACG TCGAGAAGAA GTGAGAAAGT CTTTTGTTGA GTGTGTAGAC 
GAGATACCAT TTGCTGATGA TGTTGAAGAC ACATACGATG ACCGCACTCC AGATGCAAGT 
GGTCTTGAAA AGTTTTACAC ACCGCCGACT AGCAAGGTCA ACCGGGATAA GCCGCCTCTC 
CACTTGGCAC TGGCTATGGA AAATGGCAAG CCCAATATCC CTGGAGTTTC CCGAACAGCA 
AAGGGTTCCC AGCATTTTTC TCCTGAAGCC AAGGAGATCG CAGAGGAGCG AATGAGGGCA 
CGGGAAAAGT CTGTGAAGAG CCAAGCTCTT AAAGATGCAA TGGCCAAGCA GCTTACAAAG 
ATGAAAGATT CTGAGGTTGC CAAGGGGGCT GTAGCAAAAG TAGCCTGGGA TATTCCTGAA 
ACAAAAGGAA AAAGCAAAAA GCAATCTAAA GCCCAGAAGG ACTCTGCAGT GAAGGCCTTG 
GAGTCCAAAA AGCAAGCAGA CACACTTCCA GACCGTTTCT TCTCTACACC ATCTAGTAAG 
GCCTTAGACA GCTCTGTCAC ATCTTCAGAG AGCTCCACAG GGGGCAAAAG CAAGAAACGC 
AGCTCACTGT TTTCTCCACG CAAAAACAAA AAAGAAAAGA AAGCCAAAAA TGAAAGACTG 
TCCAGCACTG AAGAAACGCC GCCCAAGCAC AAGTCCCTTT GGAAAGCAGT GTTCTCCGGC 
TACAAGAAGG ACAAAAAGAA GAAAGATGAC AAGTCATGCC CAAGTACACC CTCAAGCTCT 
ACAACTGGAG ACTCGGGGAA GAAGAAAGAT TCACCCCTGG ACAGGTCCTC AGATTTACGT 
CTGAGGAGAA ATCTAAGCTT TTCTGAGGAC TCTGATCTCT CCTGTGACGA TGTTCTGGAA 
AGATCCTCTC AGAAGTCCAA GGGTGATCGT CATGTGGACC TGTTTGACTT GGTGTCAGAT 
TTGAAAAAAG AAGCCATTAA AGAGGAGAAA GCAAAAGAGA AGGCTAAGGA TAGGGCAAAG 
GAAAGGGGAA GAGTTACTGA GAAGGGCAAG GAAAGGTCAA GAGAAAGGGA GAATGAAAAA 
AGAGTCAAAG AAAAGATGGT GAAAATGAAA GATGTGGAAC GGGAGAAAGG AAAAGAGGAA 
GAGTCTGTTT ATGTACCTCA TGCATTGGCG TTCAAGAGAT CATATGCCAC AAAGAAGACA 
TACACGGAGG AGGAACTGAA CGCCAAGCTG ACAAGAAGAG TACAGAAAGC TGCACGGAGG 
CAGGCCAAGC AGGAGGAGCT CAAGAGACTT CACAGAGCTC AGATGATTCA GAGGCAGCTG 
GAGCAGGTGG AAGAGAAACA GAGGCAGTTA GAGGAGAGAG GCGTCGCGGT GGAAAAGGCA 
CTGCGAGGCG AAGCAGGAAT GGGTAAAAAG GACGATCCGA AGCTGATGCA GGAATGGTTT 
AAACTGGTCC AGGAGAAGAA TGCCTTGGTA CGATATGAAT CCGAGCTGAT GATATTTGCT 
CGGGAGCTAG AGTTGGAGGA CAGACAGAGT CGACTGCAAC AGGAACTACG GGAACGCATG 
GCAATAGATG ATCATCTGAA GACAGAGGAG GAGCTGGCAG AGGAGAAGCA GATTCTGAAT 
GAGATGTTGG AGGTGGTGGA GCAGCGAGAC TCTCTGGTGG CCCTGCTGGA GGAACAGAGA 
CTCAGGGAGA AGGAAGAAGA TAAAGACCTG GAGGCGGTGA TGCTCTCCAA AGGATTCAAC 
CTCAACTGGG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3A ( XM_021467762.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021323437.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X3, mRNA.

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CloneID	ODa145983
Clone ID Related Accession (Same CDS sequence)	XM_021473239.1 , XM_021467762.1
Accession Version	XM_021467762.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3a isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAGTCC 
ATCGTGCGCT CCAGTAAGCT GCTGAACTGG TGTCAGAGGC AGACGGAAGG CTACCGAGGT 
GTCAGCGTGT CTGATCTCAC CACGTCCTGG AAGAGTGGTT TGGCCCTGTG CGCGCTCATC 
CACAGATACA GACCAGACCT CATTGACTTT GAATCACTGG ACGAGAAAGA CGTGGAGAAG 
AACAACCAGC TGGCTTTCGA CGTGGCTGAA AGAGAGTTTG GGATTTCTCC CATCATGACC 
GGGAAGGAGA TGTCTGTTGT GGTGGAGCCA GATAAGCTCT CCATGGTGAT GTACCTCAGT 
CAGTTCTACG AGATGTTCAA GGACACGGTG CCACCTGGTG AAAACCAGAA TCTGAGCCCT 
GAGGAGAAGG CCGCACTGAT CGCCAGCACT AAATCACCCA TCTCATTCCT TAGTAAGCTA 
GGTCAAAGCA TCGCAATCTC CCGCAAAAGA AACCCCAAGG ATAAAAAAGA AAAGGAGCTG 
GATGGACTAG GGAAAAGACG AAAGACGAGC CAGGCTGGAC AATCGGAGGA CGAGGAGCTG 
CAGAGGGCTA ATCGAGACGA CAGGCCATCT ATAGCCACAG CTCTGGCAGA ACGTAAGATC 
GACTCCGCTG CAGCAGCCAA CAATAACAAC AAAGTGAAGT CGATGGCCAC GCAGCTGCTG 
GCCAAGTTTG AGGAGAACGC GCCGACACAG TCCACTGGGC TCAAACGACA GGGTTCCTTT 
CGAAAGGAGT TCCCTCAGAA CATCGGTGGC AGCGATGTGT GTTTCTTCTG CCGTAAACGG 
GTCTATGTGA TGGAGCGTCT CAGCGCTGAA GGAAAGTTTT TCCATCGCAG CTGCTTCAAG 
TGCGACTACT GCGGCACAAC CCTCCGCCTG TCCTCATATG CCTTCGATGT TGAAGACGGG 
AAGTTTTATT GTAAACCACA CTACTGCTAC CGACTCTCTG GTGTGGCACA GAGAAAACGG 
CCTGCACCTG CTGCTGCTCC AGCAAACGCC AAGGAGCCCC AGGTGTTGGC GGTATCCCCC 
AACACAGTGG ACGCCCCAGG TCAAGCCATA ACCACTCAGA CCCCTGCGGA GCGCCGGCCC 
TCAGAAACTG AGGTAAATGG TGTCACGGAG CCCAGCGTGG CCAAACGTCT GAAAGGGACT 
CCGGAAAGGA TCGAGCTGGA GAACTATCGT TTGTCAATGA TGAGGGAGGA GGAGCTGGAG 
GAGGTGCCGG AGGAGACATT GGCGGAGCAC AACCTCAGCA GTGTGCTGGA TAAAGCCACC 
GACATCGAGG AGGGATCCAG CAGCTCTGAG TCTGACATGG AGGAAGAGGA TGAAGATGCA 
GAGGCGGCCG GTCCGTCTGA TCTGGGTGGA GTTCCCTGGA AGGAGGCAGT AGAGCTCCAT 
GCTAAGTTAA AGGGAGAAAG CGACCCAGGA GCTGACGATG ATGGCCTTCA TGACGGTGAT 
GGAGAGATGG ACGAGGATGA AGAAGAGGAG GAAGATGAGG AAGATGAAGA TGAGGAGGAA 
GAAGAGGAAT CGAGCGAAGC TAGGAACCTG CAGATCCAGC AAGCCATTCA TCCAGTAGAC 
CCTCTGGACA TCCAGATGGA GAGCCATCAG GCTGAGGGGG TGTTGGAGAT GCCCTCGGTG 
TCACCCTTAG GCTCACAGCC CTCGCTCAAC ACCCAGCTCA CACAGCCCAC CTCCTCCACA 
GCCCCCTCCC ACAAATCCGT ACGTCACGAG GCTGTCAGAG CCTGGCTGGA CTCGATGTCT 
GGAGAGCCCT GTGAAGAGGA CGATGACCCT GAAGCTGAAG CAGGCAGTCC TGACTTTGAG 
CCAGGCACTG AAATAGATCA AGAGGACATT CCCTCAGATG CAGAGGCTGA AGCTCGTTCA 
CGGTGCATAG ATGAAGTGGT GACACTTCCA GTGGATAATG ATAAAACAGA AAGCCAGGGA 
CAGGTTTTCA ACACTGCAGA GAAAACGTCT GCAAATCCAA GGGAGTTGAT TGTGGACGTT 
GTGCTGTCTC CAATCCAAAA ACCTGCTTTA CCCATTGAGG AGGTGCATGA AGTTTCTCCT 
GTAGTACTAG TTAAATCTCC TGGTGCACGT TTCTTCCCTG AGCCCTATCT GCCTGATAAA 
GTCAAGCAGA ACATTCCCCC GCCTCAGAGT CCTGATGTTA AAACCCCTCA TTCTCCTGTA 
GCTCAAATCA TTGCGCTGTC GCCCATCTGT TCCCAGCCTG TTCCACAACC AGGCACAGCA 
TCTCCCAAAT CTCCAGTCCA ACCACAGCCT TGTGCCTGTT CTCCAACAGG CAATCCTTTG 
TCTCCGATTT GTACCCAATC GCAACCCTGC AATGAGCCAC CTTCACCCCT ATCCACAAGC 
TCCCCCGTCA GGACTCAACC AGTCCCAGCA GTAACTTCTA CTCCTTTAGC TAAACCTGCT 
TCCGAGAATA GGACTAATGA ACATTTGAAA GATTCAACTC CTGAGCTTAA GAAAACAGAC 
CTCATTGAGG AGTTCTGGTT GAAGAGTGCA GAGATTAGAA AAAGTTTAGG GCTCACTCCC 
CTGGAACGAA GCAAAACTGC GGTGGAGAAG AGCATTGTGA AAACTCCAAC ACCCGAATCA 
TCATCTCCTA AGTCTTATAC CCCAGAGGAC TTGTCTGAGG AACAGAAGCC TACATTTACG 
GGCCGTTCTA TCATACGCAG GATCAATATT ACCTTGGAGG GTCAGGTTAT ATCTCCGGTG 
GAACCTAAAA GTAATGGTTC AGAAAAGAAG GACTTAAGCA GCAGTTCAGG GTTGGGTCTA 
AACGGAAGTG TGACCACCAG TCAGACAGCT GCTAGCGACA GCTACAACAA CTCTGATTCT 
ACAATGCTTA CCCCTCCTTC CAGTCCACCA CCTCCTCCAC CCAGGGAGGA ACCTGCTTGT 
CTCCAAAACA AGAAGTCTCA GGTTTCCTGG GACAACCTCC TTGAAGGCAC AGAAGAACCC 
AAATCTGAGA CCATGCCCAT TAAGCCCAGG ACTCCAGTTA GTCCCCCTCA ACCAAAACAA 
AAACCAGTTA CAGCTCCAGT GCCAACCCCT AGAACCAATC CACCTGTTGT GATGAGGGTT 
AAGGAGCCCA ATAAACCACG TCGAGAAGAA GTGAGAAAGT CTTTTGTTGA GTGTGTAGAC 
GAGATACCAT TTGCTGATGA TGTTGAAGAC ACATACGATG ACCGCACTCC AGATGCAAGT 
GGTCTTGAAA AGTTTTACAC ACCGCCGACT AGCAAGGTCA ACCGGGATAA GCCGCCTCTC 
CACTTGGCAC TGGCTATGGA AAATGGCAAG CCCAATATCC CTGGAGTTTC CCGAACAGCA 
AAGGGTTCCC AGCATTTTTC TCCTGAAGCC AAGGAGATCG CAGAGGAGCG AATGAGGGCA 
CGGGAAAAGT CTGTGAAGAG CCAAGCTCTT AAAGATGCAA TGGCCAAGCA GCTTACAAAG 
ATGAAAGATT CTGAGGTTGC CAAGGGGGCT GTAGCAAAAG TAGCCTGGGA TATTCCTGAA 
ACAAAAGGAA AAAGCAAAAA GCAATCTAAA GCCCAGAAGG ACTCTGCAGT GAAGGCCTTG 
GAGTCCAAAA AGCAAGCAGA CACACTTCCA GACCGTTTCT TCTCTACACC ATCTAGTAAG 
GCCTTAGACA GCTCTGTCAC ATCTTCAGAG AGCTCCACAG GGGGCAAAAG CAAGAAACGC 
AGCTCACTGT TTTCTCCACG CAAAAACAAA AAAGAAAAGA AAGCCAAAAA TGAAAGACTG 
TCCAGCACTG AAGAAACGCC GCCCAAGCAC AAGTCCCTTT GGAAAGCAGT GTTCTCCGGC 
TACAAGAAGG ACAAAAAGAA GAAAGATGAC AAGTCATGCC CAAGTACACC CTCAAGCTCT 
ACAACTGGAG ACTCGGGGAA GAAGAAAGAT TCACCCCTGG ACAGGTCCTC AGATTTACGT 
CTGAGGAGAA ATCTAAGCTT TTCTGAGGAC TCTGATCTCT CCTGTGACGA TGTTCTGGAA 
AGATCCTCTC AGAAGTCCAA GGGTGATCGT CATGTGGACC TGTTTGACTT GGTGTCAGAT 
TTGAAAAAAG AAGCCATTAA AGAGGAGAAA GCAAAAGAGA AGGCTAAGGA TAGGGCAAAG 
GAAAGGGGAA GAGTTACTGA GAAGGGCAAG GAAAGGTCAA GAGAAAGGGA GAATGAAAAA 
AGAGTCAAAG AAAAGATGGT GAAAATGAAA GATGTGGAAC GGGAGAAAGG AAAAGAGGAA 
GAGTCTGTTT ATGTACCTCA TGCATTGGCG TTCAAGAGAT CATATGCCAC AAAGAAGACA 
TACACGGAGG AGGAACTGAA CGCCAAGCTG ACAAGAAGAG TACAGAAAGC TGCACGGAGG 
CAGGCCAAGC AGGAGGAGCT CAAGAGACTT CACAGAGCTC AGATGATTCA GAGGCAGCTG 
GAGCAGGTGG AAGAGAAACA GAGGCAGTTA GAGGAGAGAG GCGTCGCGGT GGAAAAGGCA 
CTGCGAGGCG AAGCAGGAAT GGGTAAAAAG GACGATCCGA AGCTGATGCA GGAATGGTTT 
AAACTGGTCC AGGAGAAGAA TGCCTTGGTA CGATATGAAT CCGAGCTGAT GATATTTGCT 
CGGGAGCTAG AGTTGGAGGA CAGACAGAGT CGACTGCAAC AGGAACTACG GGAACGCATG 
GCAATAGATG ATCATCTGAA GACAGAGGAG GAGCTGGCAG AGGAGAAGCA GATTCTGAAT 
GAGATGTTGG AGGTGGTGGA GCAGCGAGAC TCTCTGGTGG CCCTGCTGGA GGAACAGAGA 
CTCAGGGAGA AGGAAGAAGA TAAAGACCTG GAGGCGGTGA TGCTCTCCAA AGGATTCAAC 
CTCAACTGGG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323437.1
CDS	528..6902
Translation	MGDGGVNAVGEGVNQSHMLFDRFVQATTCKGTLKAFQELCDFLELKPNEYRVFYHKLKSKLNYWKAKALWAKLDKRASHKEYKKGRACANTKCLIIGAGPCGLRTAIELGFLGAKVVLLEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGIEIHVNVEFKGLIEPPEDQENERIGWRAEVHPRTHPVNELEFDVIIGADGRRNTLSGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILHDYADTEMLLSRANVDQKALLSYAREAADFSTNHQLPKLDFAINHYGQPDVAMFDFTCMYASENAALVRQRNGHKLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWAHGSSPLEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNISLHQVRPNQVRHLLDTGETRDLRVDLENVVNSSTPKLTRNESIVRSSKLLNWCQRQTEGYRGVSVSDLTTSWKSGLALCALIHRYRPDLIDFESLDEKDVEKNNQLAFDVAEREFGISPIMTGKEMSVVVEPDKLSMVMYLSQFYEMFKDTVPPGENQNLSPEEKAALIASTKSPISFLSKLGQSIAISRKRNPKDKKEKELDGLGKRRKTSQAGQSEDEELQRANRDDRPSIATALAERKIDSAAAANNNNKVKSMATQLLAKFEENAPTQSTGLKRQGSFRKEFPQNIGGSDVCFFCRKRVYVMERLSAEGKFFHRSCFKCDYCGTTLRLSSYAFDVEDGKFYCKPHYCYRLSGVAQRKRPAPAAAPANAKEPQVLAVSPNTVDAPGQAITTQTPAERRPSETEVNGVTEPSVAKRLKGTPERIELENYRLSMMREEELEEVPEETLAEHNLSSVLDKATDIEEGSSSSESDMEEEDEDAEAAGPSDLGGVPWKEAVELHAKLKGESDPGADDDGLHDGDGEMDEDEEEEEDEEDEDEEEEEESSEARNLQIQQAIHPVDPLDIQMESHQAEGVLEMPSVSPLGSQPSLNTQLTQPTSSTAPSHKSVRHEAVRAWLDSMSGEPCEEDDDPEAEAGSPDFEPGTEIDQEDIPSDAEAEARSRCIDEVVTLPVDNDKTESQGQVFNTAEKTSANPRELIVDVVLSPIQKPALPIEEVHEVSPVVLVKSPGARFFPEPYLPDKVKQNIPPPQSPDVKTPHSPVAQIIALSPICSQPVPQPGTASPKSPVQPQPCACSPTGNPLSPICTQSQPCNEPPSPLSTSSPVRTQPVPAVTSTPLAKPASENRTNEHLKDSTPELKKTDLIEEFWLKSAEIRKSLGLTPLERSKTAVEKSIVKTPTPESSSPKSYTPEDLSEEQKPTFTGRSIIRRINITLEGQVISPVEPKSNGSEKKDLSSSSGLGLNGSVTTSQTAASDSYNNSDSTMLTPPSSPPPPPPREEPACLQNKKSQVSWDNLLEGTEEPKSETMPIKPRTPVSPPQPKQKPVTAPVPTPRTNPPVVMRVKEPNKPRREEVRKSFVECVDEIPFADDVEDTYDDRTPDASGLEKFYTPPTSKVNRDKPPLHLALAMENGKPNIPGVSRTAKGSQHFSPEAKEIAEERMRAREKSVKSQALKDAMAKQLTKMKDSEVAKGAVAKVAWDIPETKGKSKKQSKAQKDSAVKALESKKQADTLPDRFFSTPSSKALDSSVTSSESSTGGKSKKRSSLFSPRKNKKEKKAKNERLSSTEETPPKHKSLWKAVFSGYKKDKKKKDDKSCPSTPSSSTTGDSGKKKDSPLDRSSDLRLRRNLSFSEDSDLSCDDVLERSSQKSKGDRHVDLFDLVSDLKKEAIKEEKAKEKAKDRAKERGRVTEKGKERSRERENEKRVKEKMVKMKDVEREKGKEEESVYVPHALAFKRSYATKKTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQMIQRQLEQVEEKQRQLEERGVAVEKALRGEAGMGKKDDPKLMQEWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAIDDHLKTEEELAEEKQILNEMLEVVEQRDSLVALLEEQRLREKEEDKDLEAVMLSKGFNLNWA

Target ORF information:

RefSeq Version	XM_021467762.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3a (mical3a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467762.1

ORF Insert Sequence:

ATGGGAGATG GAGGCGTCAA TGCGGTGGGA GAAGGGGTGA ATCAGTCCCA TATGCTTTTT 
GACCGCTTCG TCCAGGCCAC CACCTGCAAA GGCACTCTCA AGGCTTTTCA AGAGCTCTGT 
GACTTCCTGG AACTCAAGCC CAATGAATAC CGCGTGTTCT ACCACAAACT CAAGTCCAAG 
CTCAACTACT GGAAAGCCAA AGCTCTCTGG GCCAAATTAG ACAAGCGCGC CAGCCACAAA 
GAATACAAAA AAGGAAGGGC CTGTGCCAAC ACTAAGTGTC TGATAATTGG AGCTGGACCA 
TGTGGTTTAC GTACAGCCAT AGAGCTGGGT TTCCTCGGGG CCAAGGTGGT TTTATTGGAG 
AAGAGAGATG CGTTCTCTCG AAACAATGTG CTCCATCTTT GGCCCTTCAC CATTCAGGAT 
CTGAGGGGTC TTGGAGCCAA GAAGTTCTAC GGCAAATTTT GTGCTGGAGC CATCGACCAT 
ATCAGTATTC GTCAGTTGCA GTTGATGCTC TTGAAGGTCG CTCTTCTCCT CGGCATCGAG 
ATTCATGTCA ACGTGGAGTT CAAAGGCCTC ATTGAGCCAC CAGAAGACCA AGAGAATGAG 
AGAATTGGGT GGAGAGCCGA GGTCCACCCC AGAACACATC CAGTCAATGA ACTAGAGTTT 
GATGTCATAA TTGGGGCTGA TGGGAGGAGA AACACACTGT CAGGGTTCCG GAGAAAGGAG 
TTCAGGGGCA AACTGGCTAT TGCCATTACA GCAAACTTCA TTAACCGCAA CACGACCGCA 
GAAGCAAAGG TGGAGGAGAT CAGCGGAGTA GCCTTCATCT TCAACCAAAA GTTCTTCCAG 
GACCTCAGAG AAGCCACAGG AATTGACCTG GAAAACATTG TCTACTATAA GGATGACACC 
CACTACTTTG TAATGACTGC CAAAAAGCAG AGCTTGCTGG AAAAGGGCGT CATTCTACAT 
GACTATGCTG ACACAGAAAT GCTGCTCTCC AGAGCCAATG TTGACCAGAA GGCTTTACTC 
TCTTACGCCA GAGAGGCAGC AGATTTTTCC ACCAATCATC AGCTTCCTAA GCTAGACTTC 
GCCATCAACC ACTACGGTCA ACCTGACGTC GCCATGTTTG ACTTCACCTG TATGTACGCC 
TCAGAGAATG CAGCGCTCGT CCGCCAGCGC AACGGCCATA AGCTGCTAGT AGCTCTTGTG 
GGAGACAGTC TTCTAGAGCC ATTCTGGCCG ATGGGCACTG GAATAGCTCG AGGTTTCTTG 
GCAGCCATGG ACTCAGCATG GATGGTGCGC AGCTGGGCTC ATGGAAGCTC TCCTCTGGAG 
GTGCTGGCTG AACGGGAAAG TATCTATCGA CTGCTGCCTC AGACGACACC AGAGAATGTG 
AGTAAGAACT TCAGCCAATA CAGCGTGGAC CCAACAACAC GATACCCCAA CATCAGCCTT 
CATCAAGTGA GACCCAACCA GGTACGGCAT CTGTTGGACA CCGGAGAAAC CAGGGATTTG 
CGTGTGGACT TGGAGAATGT GGTCAACTCT TCAACTCCTA AACTCACAAG AAATGAGTCC 
ATCGTGCGCT CCAGTAAGCT GCTGAACTGG TGTCAGAGGC AGACGGAAGG CTACCGAGGT 
GTCAGCGTGT CTGATCTCAC CACGTCCTGG AAGAGTGGTT TGGCCCTGTG CGCGCTCATC 
CACAGATACA GACCAGACCT CATTGACTTT GAATCACTGG ACGAGAAAGA CGTGGAGAAG 
AACAACCAGC TGGCTTTCGA CGTGGCTGAA AGAGAGTTTG GGATTTCTCC CATCATGACC 
GGGAAGGAGA TGTCTGTTGT GGTGGAGCCA GATAAGCTCT CCATGGTGAT GTACCTCAGT 
CAGTTCTACG AGATGTTCAA GGACACGGTG CCACCTGGTG AAAACCAGAA TCTGAGCCCT 
GAGGAGAAGG CCGCACTGAT CGCCAGCACT AAATCACCCA TCTCATTCCT TAGTAAGCTA 
GGTCAAAGCA TCGCAATCTC CCGCAAAAGA AACCCCAAGG ATAAAAAAGA AAAGGAGCTG 
GATGGACTAG GGAAAAGACG AAAGACGAGC CAGGCTGGAC AATCGGAGGA CGAGGAGCTG 
CAGAGGGCTA ATCGAGACGA CAGGCCATCT ATAGCCACAG CTCTGGCAGA ACGTAAGATC 
GACTCCGCTG CAGCAGCCAA CAATAACAAC AAAGTGAAGT CGATGGCCAC GCAGCTGCTG 
GCCAAGTTTG AGGAGAACGC GCCGACACAG TCCACTGGGC TCAAACGACA GGGTTCCTTT 
CGAAAGGAGT TCCCTCAGAA CATCGGTGGC AGCGATGTGT GTTTCTTCTG CCGTAAACGG 
GTCTATGTGA TGGAGCGTCT CAGCGCTGAA GGAAAGTTTT TCCATCGCAG CTGCTTCAAG 
TGCGACTACT GCGGCACAAC CCTCCGCCTG TCCTCATATG CCTTCGATGT TGAAGACGGG 
AAGTTTTATT GTAAACCACA CTACTGCTAC CGACTCTCTG GTGTGGCACA GAGAAAACGG 
CCTGCACCTG CTGCTGCTCC AGCAAACGCC AAGGAGCCCC AGGTGTTGGC GGTATCCCCC 
AACACAGTGG ACGCCCCAGG TCAAGCCATA ACCACTCAGA CCCCTGCGGA GCGCCGGCCC 
TCAGAAACTG AGGTAAATGG TGTCACGGAG CCCAGCGTGG CCAAACGTCT GAAAGGGACT 
CCGGAAAGGA TCGAGCTGGA GAACTATCGT TTGTCAATGA TGAGGGAGGA GGAGCTGGAG 
GAGGTGCCGG AGGAGACATT GGCGGAGCAC AACCTCAGCA GTGTGCTGGA TAAAGCCACC 
GACATCGAGG AGGGATCCAG CAGCTCTGAG TCTGACATGG AGGAAGAGGA TGAAGATGCA 
GAGGCGGCCG GTCCGTCTGA TCTGGGTGGA GTTCCCTGGA AGGAGGCAGT AGAGCTCCAT 
GCTAAGTTAA AGGGAGAAAG CGACCCAGGA GCTGACGATG ATGGCCTTCA TGACGGTGAT 
GGAGAGATGG ACGAGGATGA AGAAGAGGAG GAAGATGAGG AAGATGAAGA TGAGGAGGAA 
GAAGAGGAAT CGAGCGAAGC TAGGAACCTG CAGATCCAGC AAGCCATTCA TCCAGTAGAC 
CCTCTGGACA TCCAGATGGA GAGCCATCAG GCTGAGGGGG TGTTGGAGAT GCCCTCGGTG 
TCACCCTTAG GCTCACAGCC CTCGCTCAAC ACCCAGCTCA CACAGCCCAC CTCCTCCACA 
GCCCCCTCCC ACAAATCCGT ACGTCACGAG GCTGTCAGAG CCTGGCTGGA CTCGATGTCT 
GGAGAGCCCT GTGAAGAGGA CGATGACCCT GAAGCTGAAG CAGGCAGTCC TGACTTTGAG 
CCAGGCACTG AAATAGATCA AGAGGACATT CCCTCAGATG CAGAGGCTGA AGCTCGTTCA 
CGGTGCATAG ATGAAGTGGT GACACTTCCA GTGGATAATG ATAAAACAGA AAGCCAGGGA 
CAGGTTTTCA ACACTGCAGA GAAAACGTCT GCAAATCCAA GGGAGTTGAT TGTGGACGTT 
GTGCTGTCTC CAATCCAAAA ACCTGCTTTA CCCATTGAGG AGGTGCATGA AGTTTCTCCT 
GTAGTACTAG TTAAATCTCC TGGTGCACGT TTCTTCCCTG AGCCCTATCT GCCTGATAAA 
GTCAAGCAGA ACATTCCCCC GCCTCAGAGT CCTGATGTTA AAACCCCTCA TTCTCCTGTA 
GCTCAAATCA TTGCGCTGTC GCCCATCTGT TCCCAGCCTG TTCCACAACC AGGCACAGCA 
TCTCCCAAAT CTCCAGTCCA ACCACAGCCT TGTGCCTGTT CTCCAACAGG CAATCCTTTG 
TCTCCGATTT GTACCCAATC GCAACCCTGC AATGAGCCAC CTTCACCCCT ATCCACAAGC 
TCCCCCGTCA GGACTCAACC AGTCCCAGCA GTAACTTCTA CTCCTTTAGC TAAACCTGCT 
TCCGAGAATA GGACTAATGA ACATTTGAAA GATTCAACTC CTGAGCTTAA GAAAACAGAC 
CTCATTGAGG AGTTCTGGTT GAAGAGTGCA GAGATTAGAA AAAGTTTAGG GCTCACTCCC 
CTGGAACGAA GCAAAACTGC GGTGGAGAAG AGCATTGTGA AAACTCCAAC ACCCGAATCA 
TCATCTCCTA AGTCTTATAC CCCAGAGGAC TTGTCTGAGG AACAGAAGCC TACATTTACG 
GGCCGTTCTA TCATACGCAG GATCAATATT ACCTTGGAGG GTCAGGTTAT ATCTCCGGTG 
GAACCTAAAA GTAATGGTTC AGAAAAGAAG GACTTAAGCA GCAGTTCAGG GTTGGGTCTA 
AACGGAAGTG TGACCACCAG TCAGACAGCT GCTAGCGACA GCTACAACAA CTCTGATTCT 
ACAATGCTTA CCCCTCCTTC CAGTCCACCA CCTCCTCCAC CCAGGGAGGA ACCTGCTTGT 
CTCCAAAACA AGAAGTCTCA GGTTTCCTGG GACAACCTCC TTGAAGGCAC AGAAGAACCC 
AAATCTGAGA CCATGCCCAT TAAGCCCAGG ACTCCAGTTA GTCCCCCTCA ACCAAAACAA 
AAACCAGTTA CAGCTCCAGT GCCAACCCCT AGAACCAATC CACCTGTTGT GATGAGGGTT 
AAGGAGCCCA ATAAACCACG TCGAGAAGAA GTGAGAAAGT CTTTTGTTGA GTGTGTAGAC 
GAGATACCAT TTGCTGATGA TGTTGAAGAC ACATACGATG ACCGCACTCC AGATGCAAGT 
GGTCTTGAAA AGTTTTACAC ACCGCCGACT AGCAAGGTCA ACCGGGATAA GCCGCCTCTC 
CACTTGGCAC TGGCTATGGA AAATGGCAAG CCCAATATCC CTGGAGTTTC CCGAACAGCA 
AAGGGTTCCC AGCATTTTTC TCCTGAAGCC AAGGAGATCG CAGAGGAGCG AATGAGGGCA 
CGGGAAAAGT CTGTGAAGAG CCAAGCTCTT AAAGATGCAA TGGCCAAGCA GCTTACAAAG 
ATGAAAGATT CTGAGGTTGC CAAGGGGGCT GTAGCAAAAG TAGCCTGGGA TATTCCTGAA 
ACAAAAGGAA AAAGCAAAAA GCAATCTAAA GCCCAGAAGG ACTCTGCAGT GAAGGCCTTG 
GAGTCCAAAA AGCAAGCAGA CACACTTCCA GACCGTTTCT TCTCTACACC ATCTAGTAAG 
GCCTTAGACA GCTCTGTCAC ATCTTCAGAG AGCTCCACAG GGGGCAAAAG CAAGAAACGC 
AGCTCACTGT TTTCTCCACG CAAAAACAAA AAAGAAAAGA AAGCCAAAAA TGAAAGACTG 
TCCAGCACTG AAGAAACGCC GCCCAAGCAC AAGTCCCTTT GGAAAGCAGT GTTCTCCGGC 
TACAAGAAGG ACAAAAAGAA GAAAGATGAC AAGTCATGCC CAAGTACACC CTCAAGCTCT 
ACAACTGGAG ACTCGGGGAA GAAGAAAGAT TCACCCCTGG ACAGGTCCTC AGATTTACGT 
CTGAGGAGAA ATCTAAGCTT TTCTGAGGAC TCTGATCTCT CCTGTGACGA TGTTCTGGAA 
AGATCCTCTC AGAAGTCCAA GGGTGATCGT CATGTGGACC TGTTTGACTT GGTGTCAGAT 
TTGAAAAAAG AAGCCATTAA AGAGGAGAAA GCAAAAGAGA AGGCTAAGGA TAGGGCAAAG 
GAAAGGGGAA GAGTTACTGA GAAGGGCAAG GAAAGGTCAA GAGAAAGGGA GAATGAAAAA 
AGAGTCAAAG AAAAGATGGT GAAAATGAAA GATGTGGAAC GGGAGAAAGG AAAAGAGGAA 
GAGTCTGTTT ATGTACCTCA TGCATTGGCG TTCAAGAGAT CATATGCCAC AAAGAAGACA 
TACACGGAGG AGGAACTGAA CGCCAAGCTG ACAAGAAGAG TACAGAAAGC TGCACGGAGG 
CAGGCCAAGC AGGAGGAGCT CAAGAGACTT CACAGAGCTC AGATGATTCA GAGGCAGCTG 
GAGCAGGTGG AAGAGAAACA GAGGCAGTTA GAGGAGAGAG GCGTCGCGGT GGAAAAGGCA 
CTGCGAGGCG AAGCAGGAAT GGGTAAAAAG GACGATCCGA AGCTGATGCA GGAATGGTTT 
AAACTGGTCC AGGAGAAGAA TGCCTTGGTA CGATATGAAT CCGAGCTGAT GATATTTGCT 
CGGGAGCTAG AGTTGGAGGA CAGACAGAGT CGACTGCAAC AGGAACTACG GGAACGCATG 
GCAATAGATG ATCATCTGAA GACAGAGGAG GAGCTGGCAG AGGAGAAGCA GATTCTGAAT 
GAGATGTTGG AGGTGGTGGA GCAGCGAGAC TCTCTGGTGG CCCTGCTGGA GGAACAGAGA 
CTCAGGGAGA AGGAAGAAGA TAAAGACCTG GAGGCGGTGA TGCTCTCCAA AGGATTCAAC 
CTCAACTGGG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MICAL3A
Entrez Gene ID	402881
Full Name	microtubule associated monooxygenase, calponin and LIM domain containing 3a
Synonyms	MICAL-3A,mical3
General protein information	Preferred Names microtubule associated monooxygenase, calponin and LIM domain containing 3a Names protein-methionine sulfoxide oxidase mical3a flavoprotein oxidoreductase MICAL3 microtubule associated monoxygenase, calponin and LIM domain containing 3a molecule interacting with CasL protein 3A
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

Rattus norvegicus (Norway rat)	Mical3	NP_001178014.1
Pan troglodytes (chimpanzee)	MICAL3	XP_001151830.2
	MICAL3	XP_001103660.2
Canis lupus familiaris (dog)	MICAL3	XP_005637453.1
Bos taurus (cattle)	MICAL3	XP_005207412.1
Danio rerio (zebrafish)	mical3a	XP_005159086.1
Homo sapiens (human)	MICAL3	NP_056056.2
Mus musculus (house mouse)	Mical3	NP_001257404.1
Gallus gallus (chicken)	MICAL3	XP_004938010.1
Xenopus tropicalis (tropical clawed frog)	mical3	XP_004912548.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MICAL3A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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