hibadhb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	hibadhb
Entrez Gene ID	394135
Full Name	3-hydroxyisobutyrate dehydrogenase b
Synonyms	hibadh,zgc:66262
General protein information	Preferred Names 3-hydroxyisobutyrate dehydrogenase b Names 3-hydroxyisobutyrate dehydrogenase, mitochondrial
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_201160.1	NP_957454.1	3-hydroxyisobutyrate dehydrogenase, mitochondrial

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Hibadh	NP_663542.1
Danio rerio (zebrafish)	hibadhb	NP_957454.1
Drosophila melanogaster (fruit fly)	CG15093	NP_725824.1
Xenopus tropicalis (tropical clawed frog)	hibadh	NP_001025604.1
Neurospora crassa	NCU06559	XP_962266.2
Arabidopsis thaliana (thale cress)	AT4G20930	NP_567617.1
Homo sapiens (human)	HIBADH	NP_689953.1
	HIBADH	XP_002803390.1
Danio rerio (zebrafish)	hibadha	NP_001003646.1
Pan troglodytes (chimpanzee)	HIBADH	XP_519013.3
Canis lupus familiaris (dog)	HIBADH	XP_536747.2
Bos taurus (cattle)	HIBADH	NP_001039571.1
Gallus gallus (chicken)	HIBADH	NP_001006362.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005581	XP_315590.3
Caenorhabditis elegans (roundworm)	CELE_B0250.5	NP_507943.1
Magnaporthe oryzae (rice blast fungus)	MGG_01687	XP_003714677.1
Rattus norvegicus (Norway rat)	Hibadh	NP_071579.1
Oryza sativa (rice)	Os06g0677400	NP_001174955.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Rbfox-regulated alternative splicing is critical for zebrafish cardiac and skeletal muscle functions.
Gallagher TL, Arribere JA, Geurts PA, Exner CR, McDonald KL, Dill KK, Marr HL, Adkar SS, Garnett AT, Amacher SL, Conboy JG
Developmental biology359(2)251-61(2011 Nov)

Tri-phasic expression of posterior Hox genes during development of pectoral fins in zebrafish: implications for the evolution of vertebrate paired appendages.
Ahn D, Ho RK
Developmental biology322(1)220-33(2008 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following hibadhb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hibadhb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02232	NM_201160.1 Latest version!	Danio rerio 3-hydroxyisobutyrate dehydrogenase b (hibadhb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hibadhb ( NM_201160.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957454.1 Danio rerio 3-hydroxyisobutyrate dehydrogenase b (hibadhb), mRNA.

Price & Availability↑

CloneID	ODa02232
Clone ID Related Accession (Same CDS sequence)	NM_201160.1
Accession Version	NM_201160.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	3-hydroxyisobutyrate dehydrogenase, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC055573.1. ##Evidence-Data-START## Transcript exon combination :: BC055573.1, EV758931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGCTC TGTTAAGGGT CTCGCGGTGT CTGGTAGTAA AGTGTAACAA CCATGTCAAC 
GCCGTGCAGG TCTCCATCAG GTCAATGGCT TCTAAAACCC AGGTGGGCTT CATTGGCCTG 
GGGAACATGG GCAACCCAAT GGCAAAGAAC CTCATAAAAC ATGGATATCC AGTGATCGCC 
ACAGATGTAT TTCCAGAATC TTGCAAAGAG CTGCAGGAAC TCGGAGCTCA GATCCTGGAT 
AGCCCTGCAG ATGTAGCAGA CAAGGCAGAT CGCATCATCA CAATGCTTCC CTCCAACCCC 
AATGTTGTAG ATGTGTATAC CGGACCAAAT GGTATTTTAA AGAAAGTGAA GAAGGGGTCT 
CTACTCATTG ATTCCAGCAC TATTGATCCA GCTGTCTCTA AAGAGATGGC AGTTGCAGCA 
GAGAAACTGG GTGCTGTTTT CATGGGTGCC CCGGTGTCTG GAGGTGTTGG TGCTGCCACT 
TCTGGTAAGC TCACTTTTAT GGTTGGTGGG CCAGAGGAAG AGTTCAATGC AGCCAAGGAG 
CTTCTGAGCT GTATGGGTGC TAATGTGGTT TACTGTGGCC AAGTTGGCAC TGGACAGGCA 
GCTAAAATCT GCAACAATAT GCTGCTGGCC ATTGGGATGA TTGGAACAGC AGAGACCATG 
AACTTGGGAA TCAGATTAGG ACTTGACCCT AAACTTCTTG CAAAGATTTT GAACATGAGC 
TCTGGCCGCT GTTGGTCAAG TGACACCTAT AATCCTGTCC CAGGTGTGAT GGAGGGAGTG 
CCCTCAGCAA ACAACTACCA GGGCGGCTTC GGCACTACAC TAATGACCAA GGATCTGGGA 
CTTGCTCAGA ATTCAGCAAC CAACACGAAA ACCCCAGTGC TGTTGGGCTC TGTAGCCCAT 
CAAATTTACC GCATGATGTG TGGACGCGGC TATGCCAACA AAGACTTCTC ATCTGTCTTC 
CAGTTTCTTC GAGAAGAGGA GGGCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957454.1
CDS	52..1041
Translation	MAALLRVSRCLVVKCNNHVNAVQVSIRSMASKTQVGFIGLGNMGNPMAKNLIKHGYPVIATDVFPESCKELQELGAQILDSPADVADKADRIITMLPSNPNVVDVYTGPNGILKKVKKGSLLIDSSTIDPAVSKEMAVAAEKLGAVFMGAPVSGGVGAATSGKLTFMVGGPEEEFNAAKELLSCMGANVVYCGQVGTGQAAKICNNMLLAIGMIGTAETMNLGIRLGLDPKLLAKILNMSSGRCWSSDTYNPVPGVMEGVPSANNYQGGFGTTLMTKDLGLAQNSATNTKTPVLLGSVAHQIYRMMCGRGYANKDFSSVFQFLREEEGQ

Target ORF information:

RefSeq Version	NM_201160.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio 3-hydroxyisobutyrate dehydrogenase b (hibadhb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201160.1

ORF Insert Sequence:

ATGGCCGCTC TGTTAAGGGT CTCGCGGTGT CTGGTAGTAA AGTGTAACAA CCATGTCAAC 
GCCGTGCAGG TCTCCATCAG GTCAATGGCT TCTAAAACCC AGGTGGGCTT CATTGGCCTG 
GGGAACATGG GCAACCCAAT GGCAAAGAAC CTCATAAAAC ATGGATATCC AGTGATCGCC 
ACAGATGTAT TTCCAGAATC TTGCAAAGAG CTGCAGGAAC TCGGAGCTCA GATCCTGGAT 
AGCCCTGCAG ATGTAGCAGA CAAGGCAGAT CGCATCATCA CAATGCTTCC CTCCAACCCC 
AATGTTGTAG ATGTGTATAC CGGACCAAAT GGTATTTTAA AGAAAGTGAA GAAGGGGTCT 
CTACTCATTG ATTCCAGCAC TATTGATCCA GCTGTCTCTA AAGAGATGGC AGTTGCAGCA 
GAGAAACTGG GTGCTGTTTT CATGGGTGCC CCGGTGTCTG GAGGTGTTGG TGCTGCCACT 
TCTGGTAAGC TCACTTTTAT GGTTGGTGGG CCAGAGGAAG AGTTCAATGC AGCCAAGGAG 
CTTCTGAGCT GTATGGGTGC TAATGTGGTT TACTGTGGCC AAGTTGGCAC TGGACAGGCA 
GCTAAAATCT GCAACAATAT GCTGCTGGCC ATTGGGATGA TTGGAACAGC AGAGACCATG 
AACTTGGGAA TCAGATTAGG ACTTGACCCT AAACTTCTTG CAAAGATTTT GAACATGAGC 
TCTGGCCGCT GTTGGTCAAG TGACACCTAT AATCCTGTCC CAGGTGTGAT GGAGGGAGTG 
CCCTCAGCAA ACAACTACCA GGGCGGCTTC GGCACTACAC TAATGACCAA GGATCTGGGA 
CTTGCTCAGA ATTCAGCAAC CAACACGAAA ACCCCAGTGC TGTTGGGCTC TGTAGCCCAT 
CAAATTTACC GCATGATGTG TGGACGCGGC TATGCCAACA AAGACTTCTC ATCTGTCTTC 
CAGTTTCTTC GAGAAGAGGA GGGCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Rbfox-regulated alternative splicing is critical for zebrafish cardiac and skeletal muscle functions.
Developmental biology359(2)251-61(2011 Nov)
Gallagher TL,Arribere JA,Geurts PA,Exner CR,McDonald KL,Dill KK,Marr HL,Adkar SS,Garnett AT,Amacher SL,Conboy JG

Tri-phasic expression of posterior Hox genes during development of pectoral fins in zebrafish: implications for the evolution of vertebrate paired appendages.
Developmental biology322(1)220-33(2008 Oct)
Ahn D,Ho RK