eif4a3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	eif4a3
Entrez Gene ID	394053
Full Name	eukaryotic translation initiation factor 4A3
Synonyms	ddx48,eIF4A-III,zgc:56139
General protein information	Preferred Names eukaryotic translation initiation factor 4A3 Names eukaryotic initiation factor 4A-III ATP-dependent RNA helicase DDX48 ATP-dependent RNA helicase eIF4A-3 DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 DEAD box protein 48 eIF-4A-III eukaryotic initiation factor 4AIII eukaryotic translation initiation factor 4A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_201078.1	NP_957372.1	eukaryotic initiation factor 4A-III

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	EIF4A3	XP_533130.2
Bos taurus (cattle)	EIF4A3	NP_001039653.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003089	XP_312776.3
Caenorhabditis elegans (roundworm)	F33D11.10	NP_491703.1
Saccharomyces cerevisiae (baker's yeast)	FAL1	NP_010304.3
Neurospora crassa	NCU01234	XP_961600.1
Arabidopsis thaliana (thale cress)	EIF4A-III	NP_188610.1
Gallus gallus (chicken)	EIF4A3	NP_001025820.1
Danio rerio (zebrafish)	eif4a3	NP_957372.1
Drosophila melanogaster (fruit fly)	eIF4AIII	NP_649788.2
Caenorhabditis elegans (roundworm)	Y65B4A.6	NP_490761.2
Oryza sativa (rice)	Os01g0639100	NP_001043673.1
Homo sapiens (human)	EIF4A3	NP_055555.1
	EIF4A3	XP_001110130.1
Mus musculus (house mouse)	Eif4a3	NP_619610.1
Rattus norvegicus (Norway rat)	Eif4a3	NP_001093628.1
Schizosaccharomyces pombe (fission yeast)	SPAC1F5.10	NP_592863.1
Oryza sativa (rice)	Os03g0566800	NP_001050506.1
Xenopus tropicalis (tropical clawed frog)	eif4a3	NP_001107349.1
Pan troglodytes (chimpanzee)	EIF4A3	XP_511724.3
Kluyveromyces lactis	KLLA0A10659g	XP_451466.1
Eremothecium gossypii	AGOS_AER408W	NP_985263.2
Magnaporthe oryzae (rice blast fungus)	MGG_04885	XP_003712368.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Processing of fish Ig heavy chain transcripts: diverse splicing patterns and unusual nonsense mediated decay.
Quiniou SM, Wilson M, Boudinot P
Developmental and comparative immunology35(9)949-58(2011 Sep)

Dynamic behavior of Arabidopsis eIF4A-III, putative core protein of exon junction complex: fast relocation to nucleolus and splicing speckles under hypoxia.
Koroleva OA, Calder G, Pendle AF, Kim SH, Lewandowska D, Simpson CG, Jones IM, Brown JW, Shaw PJ
The Plant cell21(5)1592-606(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following eif4a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eif4a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02438	NM_201078.1 Latest version!	Danio rerio eukaryotic translation initiation factor 4A3 (eif4a3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eif4a3 ( NM_201078.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957372.1 Danio rerio eukaryotic translation initiation factor 4A3 (eif4a3), mRNA.

Price & Availability↑

CloneID	ODa02438
Clone ID Related Accession (Same CDS sequence)	NM_201078.1
Accession Version	NM_201078.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Danio rerio(zebrafish)
Product	eukaryotic initiation factor 4A-III
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC045939.1. ##Evidence-Data-START## Transcript exon combination :: BC045939.1, BC165833.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476735, SAMEA4476808 [ECO:0000348] ##Evidence-Data-END##

ATGGCCACCG CCGTCGTTCC CGTGAGGAAG AGGATCCTGA AGGAGGAAGA CATGACGAAG 
ATTGAGTTTG AGACGAGTGA AGAAGTGGAC GTGACGCCGA CGTTTGACAC GATGGGCCTG 
AGGGAGGATT TACTGCGCGG GATCTACGCA TACGGTTTTG AGAAGCCATC GGCCATCCAG 
CAGAGAGCCA TCAAACAGAT CATCAAGGGC AGAGACGTCA TCGCACAGTC ACAGTCTGGA 
ACTGGTAAAA CAGCCACCTT CTGTGTCTCA GTGCTGCAGT GTCTGGATAT ACAGGTACGA 
GAGACTCAAG CGTTGATTTT GGCTCCAACC AGAGAGTTAG CAGGACAGAT CCAGAAGGTT 
CTTCTGGCTC TGGGCGACTA CATGAACGTT CAGTGTCACG CGTGTATCGG CGGCACAAAT 
GTCGGTGAAG ACATCCGGAA GCTGGATTAC GGTCAGCACG TGGTGGCAGG AACGCCTGGA 
CGAGTGTTCG ACATGATCCG CAGGAGGAGT TTAAGGACTC GCGCCATCAA GATGCTGGTG 
TTGGATGAAG CCGATGAGAT GCTCAACAAG GGTTTTAAGG AACAGATTTA CGACGTGTAC 
CGATACCTGC CACCAGCCAC TCAGGTGTGT CTGATCAGCG CCACACTGCC ACACGAGATC 
CTGGAGATGA CCAACAAGTT CATGACCGAC CCCATCCGCA TCCTGGTCAA ACGTGATGAG 
TTGACTCTGG AGGGCATTAA GCAGTTCTTC GTGGCCGTGG AGCGAGAGGA GTGGAAGTTC 
GACACTCTGT GTGATCTGTA CGACACGCTG ACCATCACAC AGGCCGTCAT CTTCTGCAAC 
ACCAAACGCA AGGTGGACTG GCTGACAGAG AAAATGAGGG AGGCAAATTT CACCGTATCC 
TCGATGCACG GAGACATGCC TCAGAAAGAG CGAGAGTCCA TTATGAAGGA GTTCAGATCT 
GGAGCCAGTC GAGTGTTGAT CTCCACAGAC GTTTGGGCCA GAGGTCTGGA TGTGTCGCAG 
GTTTCCCTCA TCATTAACTA CGATCTTCCC AACAACAGAG AGCTGTACAT CCACAGGATT 
GGTCGATCGG GTCGTTACGG CAGGAAGGGT GTGGCCATCA ACTTTGTGAA GAATGACGAC 
ATCCGTATCC TGCGTGATAT CGAGCAGTAC TACTCCACAC AGATCGATGA AATGCCCATG 
AACGTGGCTG ATCTGATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957372.1
CDS	115..1335
Translation	MATAVVPVRKRILKEEDMTKIEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFCVSVLQCLDIQVRETQALILAPTRELAGQIQKVLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQVCLISATLPHEILEMTNKFMTDPIRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSMHGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVSQVSLIINYDLPNNRELYIHRIGRSGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI

Target ORF information:

RefSeq Version	NM_201078.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 4A3 (eif4a3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201078.1

ORF Insert Sequence:

ATGGCCACCG CCGTCGTTCC CGTGAGGAAG AGGATCCTGA AGGAGGAAGA CATGACGAAG 
ATTGAGTTTG AGACGAGTGA AGAAGTGGAC GTGACGCCGA CGTTTGACAC GATGGGCCTG 
AGGGAGGATT TACTGCGCGG GATCTACGCA TACGGTTTTG AGAAGCCATC GGCCATCCAG 
CAGAGAGCCA TCAAACAGAT CATCAAGGGC AGAGACGTCA TCGCACAGTC ACAGTCTGGA 
ACTGGTAAAA CAGCCACCTT CTGTGTCTCA GTGCTGCAGT GTCTGGATAT ACAGGTACGA 
GAGACTCAAG CGTTGATTTT GGCTCCAACC AGAGAGTTAG CAGGACAGAT CCAGAAGGTT 
CTTCTGGCTC TGGGCGACTA CATGAACGTT CAGTGTCACG CGTGTATCGG CGGCACAAAT 
GTCGGTGAAG ACATCCGGAA GCTGGATTAC GGTCAGCACG TGGTGGCAGG AACGCCTGGA 
CGAGTGTTCG ACATGATCCG CAGGAGGAGT TTAAGGACTC GCGCCATCAA GATGCTGGTG 
TTGGATGAAG CCGATGAGAT GCTCAACAAG GGTTTTAAGG AACAGATTTA CGACGTGTAC 
CGATACCTGC CACCAGCCAC TCAGGTGTGT CTGATCAGCG CCACACTGCC ACACGAGATC 
CTGGAGATGA CCAACAAGTT CATGACCGAC CCCATCCGCA TCCTGGTCAA ACGTGATGAG 
TTGACTCTGG AGGGCATTAA GCAGTTCTTC GTGGCCGTGG AGCGAGAGGA GTGGAAGTTC 
GACACTCTGT GTGATCTGTA CGACACGCTG ACCATCACAC AGGCCGTCAT CTTCTGCAAC 
ACCAAACGCA AGGTGGACTG GCTGACAGAG AAAATGAGGG AGGCAAATTT CACCGTATCC 
TCGATGCACG GAGACATGCC TCAGAAAGAG CGAGAGTCCA TTATGAAGGA GTTCAGATCT 
GGAGCCAGTC GAGTGTTGAT CTCCACAGAC GTTTGGGCCA GAGGTCTGGA TGTGTCGCAG 
GTTTCCCTCA TCATTAACTA CGATCTTCCC AACAACAGAG AGCTGTACAT CCACAGGATT 
GGTCGATCGG GTCGTTACGG CAGGAAGGGT GTGGCCATCA ACTTTGTGAA GAATGACGAC 
ATCCGTATCC TGCGTGATAT CGAGCAGTAC TACTCCACAC AGATCGATGA AATGCCCATG 
AACGTGGCTG ATCTGATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Processing of fish Ig heavy chain transcripts: diverse splicing patterns and unusual nonsense mediated decay.
Developmental and comparative immunology35(9)949-58(2011 Sep)
Quiniou SM,Wilson M,Boudinot P

Dynamic behavior of Arabidopsis eIF4A-III, putative core protein of exon junction complex: fast relocation to nucleolus and splicing speckles under hypoxia.
The Plant cell21(5)1592-606(2009 May)
Koroleva OA,Calder G,Pendle AF,Kim SH,Lewandowska D,Simpson CG,Jones IM,Brown JW,Shaw PJ