nt5e cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nt5e
Entrez Gene ID	393906
Full Name	5'-nucleotidase, ecto (CD73)
Synonyms	zgc:63784
General protein information	Preferred Names 5'-nucleotidase, ecto (CD73) Names 5'-nucleotidase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200932.1	NP_957226.1	5'-nucleotidase precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NT5E	XP_518619.3
Gallus gallus (chicken)	NT5E	XP_004940454.1
Danio rerio (zebrafish)	nt5e	NP_957226.1
Drosophila melanogaster (fruit fly)	NT5E-2	NP_611218.1
Xenopus tropicalis (tropical clawed frog)	nt5e	XP_002933973.1
Canis lupus familiaris (dog)	NT5E	XP_532221.2
Rattus norvegicus (Norway rat)	Nt5e	NP_067587.2
Drosophila melanogaster (fruit fly)	veil	NP_611217.1
Homo sapiens (human)	NT5E	NP_002517.1
	NT5E	XP_001086989.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003629	XP_313394.4
Mus musculus (house mouse)	Nt5e	NP_035981.1
Magnaporthe oryzae (rice blast fungus)	MGG_12474	XP_003715781.1
Neurospora crassa	NCU09659	XP_958725.1

Gene Ontology
Bibliography

Related articles in PubMed

Analysis of Extracellular Nucleotide Metabolism in Adult Zebrafish After Embryological Exposure to Valproic Acid.
Zimmermann FF, Gaspary KV, Siebel AM, Leite CE, Kist LW, Bogo MR, Bonan CD
Molecular neurobiology54(5)3542-3553(2017 Jul)

An Adenosine Receptor for Olfaction in Fish.
Wakisaka N, Miyasaka N, Koide T, Masuda M, Hiraki-Kajiyama T, Yoshihara Y
Current biology : CB27(10)1437-1447.e4(2017 May)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Hyperglycemia alters E-NTPDases, ecto-5'-nucleotidase, and ectosolic and cytosolic adenosine deaminase activities and expression from encephala of adult zebrafish (Danio rerio).
Capiotti KM, Siebel AM, Kist LW, Bogo MR, Bonan CD, Da Silva RS
Purinergic signalling12(2)211-20(2016 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

enzymatic properties of an ecto-5'-nucleotidase activity in brain membranes of zebrafish
Title: Ecto-5'-nucleotidase activity in brain membranes of zebrafish (Danio rerio).

The following nt5e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nt5e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35374	NM_200932.1 Latest version!	Danio rerio 5'-nucleotidase, ecto (CD73) (nt5e), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nt5e ( NM_200932.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957226.1 Danio rerio 5'-nucleotidase, ecto (CD73) (nt5e), mRNA.

Price & Availability↑

CloneID	ODa35374
Clone ID Related Accession (Same CDS sequence)	NM_200932.1
Accession Version	NM_200932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-30
Organism	Danio rerio(zebrafish)
Product	5'-nucleotidase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC055243.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC055243.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGATGATGA TGAATGTCCT CTCCGCGTTG CTGCTGCTCT TCATTCACTG TCAGCTGTGC 
TGGTCTGCGG ACTTCCAGCT CACTTTGCTG CACACCAATG ACGTGCACGC GCGAGTGGAG 
GAGACCAACA AGGACTCGGG CAAATGCAGC AAGCCGCCGT GTTTCGCCGG AGTGTCGCGG 
AGATCCACCA AAATCAAAGA AATCCGCAGC AAGGAGAAAA ACGTGCTGCT GCTGGACGCA 
GGAGACCAGT TCCAGGGCAC CGTCTGGTTT AACTATTACA AGGGCGCTGA GGCGGCGTAT 
TTCATGAACC AGCTCAAATA CGACGCGATG GCTTTAGGAA ACCATGAGTT TGACAACGGC 
GTGGACGGCC TGCTGAAACC TTTCCTTCAG GAGGTGAACT GCACTGTTCT CAGCGCCAAC 
ATCAAAGCTG ATGAGACACT TGCTCCTCGG ATCAGCGGAT ACTATTTCCC ATATAAAATC 
TTCACGTTGG GCTCAGAGAA AGTGGGTGTT GTCGGCTACA CATCGGCGGA GACGCCTGCT 
TTGTCTCTAC CAGGCCCACA TCTGAAGTTT GAGGATGAAA TCACGGCTCT ACAGCCTCAA 
GTGGATAAAC TCTTGACTTT GGGTGTTAAT AAGATCATCG CTCTGGGACA TTCTGGCTTC 
CTAATGGATC AAATGATCGC GAAGAAGGTT CAGGGAGTGG ACGTTGTGAT TGGAGGACAC 
ACCAATACAT TCTTGTACAC GGGAGATCCA CCGTCCACAG AAGTTCCCGC AGGGCCGTAT 
CCACTCATGG TCAAGTCAGA CGACGGCCGG CAGGTGCCTG TGGTCCAGGC TTATGCCTTT 
GGAAAATATC TGGGATTCCT GAAAGTGACT TTTGATGCAA ACGGAAATGT GCTGGAGTCG 
ACAGGAAACC CCATTCTTCT GAATAGTTCA GTAGAACCCG ATCCCGACAT CCAGGCTAAA 
GTGGACAGCT GGAGGATGAA TCTGGCCAAC TACTCCTCTC AGCAAGTGGG ACAGACTCTA 
GTCTTCCTCA ACGGCACCTT CGAGGAGTGT CGCTTTCGTG AATGCAATTT GGGGAATTTA 
ATCTGTGATG CCATGGTCCA TCATAATATT AAATACGCCG ACGAGCTCCA GTGGAACCAT 
GTCAGCTCTT GTATTCTAAA CGGTGGAGGC ATTCGAGGAC CTATTGATGA GCGAAACAGA 
AACGGTTCCA TCACAATGGA GGACCTGATC GCCGTGCTGC CGTTTGGAGG AACATTCGAC 
CTGGTCCAGC TGAACGGATC GACTCTATTA GAAGCTTTTG AGCACTCAGT TCGCCGACAC 
GGAGGAAACA CTGGAGAATT CCTACAGGTG TCAGGTTTTC AGGTGGTGTA TGATTTATCG 
AAAGCGCCTG GTAGCCGTGT TAAAAGTGTG AAAGTGCTCT GCACCCAGTG TCGAGTGCCT 
CATTATGAAC CGCTGGTCCC CAACAAGGTG TATAAAGTAG TGCTGCCGTC TTACCTAGTG 
GACGGAGGAG ACGGATTCAC CATGATCAAA GAGAAGAAAC TCAAACACGA CAGCGGTGAT 
CTGGATATAT CGGTTGTTGC TGGCTACATC TCCGAGAGGA AGAGAGTTCA TCCGGCTGTG 
GAGGGACGCT TCCAGTTCAG CAGCTCCTGT GCTGGTCTTC GGGGATACAC GTCCACCGTT 
CTGCTGCTGT GGGCCGTCTG GCTCATGCTT GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957226.1
CDS	20..1735
Translation	MMMMNVLSALLLLFIHCQLCWSADFQLTLLHTNDVHARVEETNKDSGKCSKPPCFAGVSRRSTKIKEIRSKEKNVLLLDAGDQFQGTVWFNYYKGAEAAYFMNQLKYDAMALGNHEFDNGVDGLLKPFLQEVNCTVLSANIKADETLAPRISGYYFPYKIFTLGSEKVGVVGYTSAETPALSLPGPHLKFEDEITALQPQVDKLLTLGVNKIIALGHSGFLMDQMIAKKVQGVDVVIGGHTNTFLYTGDPPSTEVPAGPYPLMVKSDDGRQVPVVQAYAFGKYLGFLKVTFDANGNVLESTGNPILLNSSVEPDPDIQAKVDSWRMNLANYSSQQVGQTLVFLNGTFEECRFRECNLGNLICDAMVHHNIKYADELQWNHVSSCILNGGGIRGPIDERNRNGSITMEDLIAVLPFGGTFDLVQLNGSTLLEAFEHSVRRHGGNTGEFLQVSGFQVVYDLSKAPGSRVKSVKVLCTQCRVPHYEPLVPNKVYKVVLPSYLVDGGDGFTMIKEKKLKHDSGDLDISVVAGYISERKRVHPAVEGRFQFSSSCAGLRGYTSTVLLLWAVWLMLV

Target ORF information:

RefSeq Version	NM_200932.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio 5'-nucleotidase, ecto (CD73) (nt5e), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200932.1

ORF Insert Sequence:

ATGATGATGA TGAATGTCCT CTCCGCGTTG CTGCTGCTCT TCATTCACTG TCAGCTGTGC 
TGGTCTGCGG ACTTCCAGCT CACTTTGCTG CACACCAATG ACGTGCACGC GCGAGTGGAG 
GAGACCAACA AGGACTCGGG CAAATGCAGC AAGCCGCCGT GTTTCGCCGG AGTGTCGCGG 
AGATCCACCA AAATCAAAGA AATCCGCAGC AAGGAGAAAA ACGTGCTGCT GCTGGACGCA 
GGAGACCAGT TCCAGGGCAC CGTCTGGTTT AACTATTACA AGGGCGCTGA GGCGGCGTAT 
TTCATGAACC AGCTCAAATA CGACGCGATG GCTTTAGGAA ACCATGAGTT TGACAACGGC 
GTGGACGGCC TGCTGAAACC TTTCCTTCAG GAGGTGAACT GCACTGTTCT CAGCGCCAAC 
ATCAAAGCTG ATGAGACACT TGCTCCTCGG ATCAGCGGAT ACTATTTCCC ATATAAAATC 
TTCACGTTGG GCTCAGAGAA AGTGGGTGTT GTCGGCTACA CATCGGCGGA GACGCCTGCT 
TTGTCTCTAC CAGGCCCACA TCTGAAGTTT GAGGATGAAA TCACGGCTCT ACAGCCTCAA 
GTGGATAAAC TCTTGACTTT GGGTGTTAAT AAGATCATCG CTCTGGGACA TTCTGGCTTC 
CTAATGGATC AAATGATCGC GAAGAAGGTT CAGGGAGTGG ACGTTGTGAT TGGAGGACAC 
ACCAATACAT TCTTGTACAC GGGAGATCCA CCGTCCACAG AAGTTCCCGC AGGGCCGTAT 
CCACTCATGG TCAAGTCAGA CGACGGCCGG CAGGTGCCTG TGGTCCAGGC TTATGCCTTT 
GGAAAATATC TGGGATTCCT GAAAGTGACT TTTGATGCAA ACGGAAATGT GCTGGAGTCG 
ACAGGAAACC CCATTCTTCT GAATAGTTCA GTAGAACCCG ATCCCGACAT CCAGGCTAAA 
GTGGACAGCT GGAGGATGAA TCTGGCCAAC TACTCCTCTC AGCAAGTGGG ACAGACTCTA 
GTCTTCCTCA ACGGCACCTT CGAGGAGTGT CGCTTTCGTG AATGCAATTT GGGGAATTTA 
ATCTGTGATG CCATGGTCCA TCATAATATT AAATACGCCG ACGAGCTCCA GTGGAACCAT 
GTCAGCTCTT GTATTCTAAA CGGTGGAGGC ATTCGAGGAC CTATTGATGA GCGAAACAGA 
AACGGTTCCA TCACAATGGA GGACCTGATC GCCGTGCTGC CGTTTGGAGG AACATTCGAC 
CTGGTCCAGC TGAACGGATC GACTCTATTA GAAGCTTTTG AGCACTCAGT TCGCCGACAC 
GGAGGAAACA CTGGAGAATT CCTACAGGTG TCAGGTTTTC AGGTGGTGTA TGATTTATCG 
AAAGCGCCTG GTAGCCGTGT TAAAAGTGTG AAAGTGCTCT GCACCCAGTG TCGAGTGCCT 
CATTATGAAC CGCTGGTCCC CAACAAGGTG TATAAAGTAG TGCTGCCGTC TTACCTAGTG 
GACGGAGGAG ACGGATTCAC CATGATCAAA GAGAAGAAAC TCAAACACGA CAGCGGTGAT 
CTGGATATAT CGGTTGTTGC TGGCTACATC TCCGAGAGGA AGAGAGTTCA TCCGGCTGTG 
GAGGGACGCT TCCAGTTCAG CAGCTCCTGT GCTGGTCTTC GGGGATACAC GTCCACCGTT 
CTGCTGCTGT GGGCCGTCTG GCTCATGCTT GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analysis of Extracellular Nucleotide Metabolism in Adult Zebrafish After Embryological Exposure to Valproic Acid.
Molecular neurobiology54(5)3542-3553(2017 Jul)
Zimmermann FF,Gaspary KV,Siebel AM,Leite CE,Kist LW,Bogo MR,Bonan CD

An Adenosine Receptor for Olfaction in Fish.
Current biology : CB27(10)1437-1447.e4(2017 May)
Wakisaka N,Miyasaka N,Koide T,Masuda M,Hiraki-Kajiyama T,Yoshihara Y

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Hyperglycemia alters E-NTPDases, ecto-5'-nucleotidase, and ectosolic and cytosolic adenosine deaminase activities and expression from encephala of adult zebrafish (Danio rerio).
Purinergic signalling12(2)211-20(2016 Jun)
Capiotti KM,Siebel AM,Kist LW,Bogo MR,Bonan CD,Da Silva RS