erp44 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	erp44
Entrez Gene ID	393866
Full Name	endoplasmic reticulum protein 44
Synonyms	txndc4,zgc:77883
General protein information	Preferred Names endoplasmic reticulum protein 44 Names endoplasmic reticulum resident protein 44 thioredoxin domain containing 4 (endoplasmic reticulum)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200892.1	NP_957186.1	endoplasmic reticulum resident protein 44

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ERP44	XP_003312248.1
Canis lupus familiaris (dog)	ERP44	XP_005627051.1
Danio rerio (zebrafish)	erp44	NP_957186.1
Xenopus tropicalis (tropical clawed frog)	erp44	NP_001011125.1
Rattus norvegicus (Norway rat)	Erp44	NP_001008318.1
Drosophila melanogaster (fruit fly)	CG9911	NP_727948.1
Caenorhabditis elegans (roundworm)	CELE_C30H7.2	NP_001040641.1
Mus musculus (house mouse)	Erp44	NP_083848.1
Homo sapiens (human)	ERP44	NP_055866.1
	LOC100423317	XP_002800093.1
Bos taurus (cattle)	ERP44	NP_001030204.1
Gallus gallus (chicken)	ERP44	XP_419079.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000909	XP_316887.5

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Transcriptional events co-regulated by hypoxia and cold stresses in Zebrafish larvae.
Long Y, Yan J, Song G, Li X, Li X, Li Q, Cui Z
BMC genomics16385(2015 May)

Endoplasmic reticulum resident protein 44 (ERp44) deficiency in mice and zebrafish leads to cardiac developmental and functional defects.
Wang DY, Abbasi C, El-Rass S, Li JY, Dawood F, Naito K, Sharma P, Bousette N, Singh S, Backx PH, Cox B, Wen XY, Liu PP, Gramolini AO
Journal of the American Heart Association3(5)e001018(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ERp44 plays a critical role in embryonic heart development and is crucial in regulating cardiac cell Ca(2+) signaling, ER stress, ROS-induced oxidative stress, and activation of the intrinsic mitochondrial apoptosis pathway.
Title: Endoplasmic reticulum resident protein 44 (ERp44) deficiency in mice and zebrafish leads to cardiac developmental and functional defects.

The following erp44 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the erp44 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36592	NM_200892.1 Latest version!	Danio rerio endoplasmic reticulum protein 44 (erp44), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

erp44 ( NM_200892.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957186.1 Danio rerio endoplasmic reticulum protein 44 (erp44), mRNA.

Price & Availability↑

CloneID	ODa36592
Clone ID Related Accession (Same CDS sequence)	NM_200892.1
Accession Version	NM_200892.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	endoplasmic reticulum resident protein 44
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC063992.1. ##Evidence-Data-START## Transcript exon combination :: BC063992.1, BC066767.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAAATTAT CATCAATAAT ACCCGTTCCC GAACTGCACT ACTTCACCCT CCTGCTGGTA 
ACGGGTCTGT ATAATCCAGG ACGAGGGGAA ATAACCAGTC TGGACTCTGG AAACATCGAT 
GACATCCTCA ACGGAGCAGG TGTGGCTCTA GTCAACTTCT ATGCAGACTG GTGTCGTTTT 
AGTCAGATGC TGCACCCCAT CTTTGAGGAG GCGTCAAATA TAGTGCGCGA GGAATATCCA 
GACGCTACCC AGGTGGTGTT CGGCCGGGTC GACTGTGACC AACACTCTGA TCTAGCGCAG 
CGCTACAGGA TAAGCAAATA CCCCACACTG AAGCTGTTCA GGAATGGCAT GATGATGAAG 
CGAGAGTACA GAGGTCAAAG GTCAGTGACC GCCATCGCCG ACTTCATCCG CCAGCAGAAA 
GTGGATCCAA TTAAAGAGAT CGAAAATCTG GAGGAAATCA GCACTGTAGA TAGGAGCAGA 
CGCACCATCA TTGGTTACTT TGAAAAAAGG GACTCGGACA ATTACCGTAC TTATGAGAAA 
GTGGCCAACA TTCTGAGAGA CGACTGTGCT TTCCTGGCAG CTTTTGGGGA GGTTTCTAAG 
CCCGAACGTT TTAGTGGTGA TAACATTATT TATAAACCAT TGGGAGAAAA TGCTCCTGAC 
ATGGTCTACA TGGGCTCGCT TACAAATTTT GACCTCAGTT ACGCCTGGAC GCAAGACAAG 
TGTGTACCGC TAGTCCGAGA AATCACATTT GAAAACGGAG AGGAACTGAC AGAGGAGGGA 
ATCCCGTTCC TGATCCTCTT CCATCAGAAG GATGACACAG AGAGTTTGGA GAAATTTCAG 
CAAGAAGTGG CTCGTCAGCT TATTAGTGAA AAGGGTTCTA TAAATTTCCT TCATGCTGAT 
TGCGATAAGT TCCGGCACCC TCTACTTCAC ATTCAGAAGA CACCTGCCGA TTGCCCTGTA 
ATCGCCATCG ATAGTTTCCG CCACATGTAT GTCTTCCCAG AGTTCAGCGA CCTTGCGGTT 
CCAGGAAAGC TGAGGCAGTT TGTATTGGAC CTGCACTCAG GAAAGTTGCA CAGAGAGTTT 
CATCACGGCC CAGACCCCAC AGACAGCACT CCAGGACAGC AAGAGGAGAA CAGAGAGGTG 
CCCAGTAATC CCCCAGAGAG CTCCTTCCAG AAATTGGCCC CCAGTGAGAC ACGCTACACC 
ATCCTCAGGG ACCGAGACGA GCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957186.1
CDS	72..1298
Translation	MKLSSIIPVPELHYFTLLLVTGLYNPGRGEITSLDSGNIDDILNGAGVALVNFYADWCRFSQMLHPIFEEASNIVREEYPDATQVVFGRVDCDQHSDLAQRYRISKYPTLKLFRNGMMMKREYRGQRSVTAIADFIRQQKVDPIKEIENLEEISTVDRSRRTIIGYFEKRDSDNYRTYEKVANILRDDCAFLAAFGEVSKPERFSGDNIIYKPLGENAPDMVYMGSLTNFDLSYAWTQDKCVPLVREITFENGEELTEEGIPFLILFHQKDDTESLEKFQQEVARQLISEKGSINFLHADCDKFRHPLLHIQKTPADCPVIAIDSFRHMYVFPEFSDLAVPGKLRQFVLDLHSGKLHREFHHGPDPTDSTPGQQEENREVPSNPPESSFQKLAPSETRYTILRDRDEL

Target ORF information:

RefSeq Version	NM_200892.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio endoplasmic reticulum protein 44 (erp44), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200892.1

ORF Insert Sequence:

ATGAAATTAT CATCAATAAT ACCCGTTCCC GAACTGCACT ACTTCACCCT CCTGCTGGTA 
ACGGGTCTGT ATAATCCAGG ACGAGGGGAA ATAACCAGTC TGGACTCTGG AAACATCGAT 
GACATCCTCA ACGGAGCAGG TGTGGCTCTA GTCAACTTCT ATGCAGACTG GTGTCGTTTT 
AGTCAGATGC TGCACCCCAT CTTTGAGGAG GCGTCAAATA TAGTGCGCGA GGAATATCCA 
GACGCTACCC AGGTGGTGTT CGGCCGGGTC GACTGTGACC AACACTCTGA TCTAGCGCAG 
CGCTACAGGA TAAGCAAATA CCCCACACTG AAGCTGTTCA GGAATGGCAT GATGATGAAG 
CGAGAGTACA GAGGTCAAAG GTCAGTGACC GCCATCGCCG ACTTCATCCG CCAGCAGAAA 
GTGGATCCAA TTAAAGAGAT CGAAAATCTG GAGGAAATCA GCACTGTAGA TAGGAGCAGA 
CGCACCATCA TTGGTTACTT TGAAAAAAGG GACTCGGACA ATTACCGTAC TTATGAGAAA 
GTGGCCAACA TTCTGAGAGA CGACTGTGCT TTCCTGGCAG CTTTTGGGGA GGTTTCTAAG 
CCCGAACGTT TTAGTGGTGA TAACATTATT TATAAACCAT TGGGAGAAAA TGCTCCTGAC 
ATGGTCTACA TGGGCTCGCT TACAAATTTT GACCTCAGTT ACGCCTGGAC GCAAGACAAG 
TGTGTACCGC TAGTCCGAGA AATCACATTT GAAAACGGAG AGGAACTGAC AGAGGAGGGA 
ATCCCGTTCC TGATCCTCTT CCATCAGAAG GATGACACAG AGAGTTTGGA GAAATTTCAG 
CAAGAAGTGG CTCGTCAGCT TATTAGTGAA AAGGGTTCTA TAAATTTCCT TCATGCTGAT 
TGCGATAAGT TCCGGCACCC TCTACTTCAC ATTCAGAAGA CACCTGCCGA TTGCCCTGTA 
ATCGCCATCG ATAGTTTCCG CCACATGTAT GTCTTCCCAG AGTTCAGCGA CCTTGCGGTT 
CCAGGAAAGC TGAGGCAGTT TGTATTGGAC CTGCACTCAG GAAAGTTGCA CAGAGAGTTT 
CATCACGGCC CAGACCCCAC AGACAGCACT CCAGGACAGC AAGAGGAGAA CAGAGAGGTG 
CCCAGTAATC CCCCAGAGAG CTCCTTCCAG AAATTGGCCC CCAGTGAGAC ACGCTACACC 
ATCCTCAGGG ACCGAGACGA GCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Transcriptional events co-regulated by hypoxia and cold stresses in Zebrafish larvae.
BMC genomics16385(2015 May)
Long Y,Yan J,Song G,Li X,Li X,Li Q,Cui Z

Endoplasmic reticulum resident protein 44 (ERp44) deficiency in mice and zebrafish leads to cardiac developmental and functional defects.
Journal of the American Heart Association3(5)e001018(2014 Oct)
Wang DY,Abbasi C,El-Rass S,Li JY,Dawood F,Naito K,Sharma P,Bousette N,Singh S,Backx PH,Cox B,Wen XY,Liu PP,Gramolini AO