aanat1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	aanat1
Entrez Gene ID	393677
Full Name	arylalkylamine N-acetyltransferase 1
Synonyms	zgc:73074
General protein information	Preferred Names arylalkylamine N-acetyltransferase 1 Names serotonin N-acetyltransferase aanat1a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200704.1	NP_956998.1	serotonin N-acetyltransferase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	AANAT	NP_001070905.1
Mus musculus (house mouse)	Aanat	NP_033721.1
Rattus norvegicus (Norway rat)	Aanat	NP_036950.1
Gallus gallus (chicken)	AANAT	NP_990489.1
Pan troglodytes (chimpanzee)	AANAT	NP_001012442.1
	AANAT	NP_001040592.1
Homo sapiens (human)	AANAT	NP_001079.1
Bos taurus (cattle)	AANAT	NP_803475.1
Danio rerio (zebrafish)	aanat1	NP_956998.1
Bos taurus (cattle)	LOC618594	XP_002696212.1
Xenopus tropicalis (tropical clawed frog)	aanat	XP_004918502.1

Gene Ontology
Bibliography

Related articles in PubMed

Molecular and functional characterization of the voltage-gated proton channel in zebrafish neutrophils.
Ratanayotha A, Kawai T, Higashijima SI, Okamura Y
Physiological reports5(15)(2017 Aug)

Melatonin biosynthesizing enzyme genes and clock genes in ovary and whole brain of zebrafish (Danio rerio): Differential expression and a possible interplay.
Khan ZA, Yumnamcha T, Rajiv C, Sanjita Devi H, Mondal G, Devi SD, Bharali R, Chattoraj A
General and comparative endocrinology23316-31(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In constant darkness, the rhythmic expression of retinal aanat2 persisted while the aanat1 rhythm disappeared
Title: Zebrafish arylalkylamine-N-acetyltransferase genes - targets for regulation of the circadian clock.

The following aanat1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the aanat1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa30844	NM_200704.1 Latest version!	Danio rerio arylalkylamine N-acetyltransferase 1 (aanat1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

aanat1 ( NM_200704.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956998.1 Danio rerio arylalkylamine N-acetyltransferase 1 (aanat1), mRNA.

Price & Availability↑

CloneID	ODa30844
Clone ID Related Accession (Same CDS sequence)	NM_200704.1
Accession Version	NM_200704.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Danio rerio(zebrafish)
Product	serotonin N-acetyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC059448.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY349158.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505378, SAMEA3505381 [ECO:0000348] ##Evidence-Data-END##

ATGTCAGTAG TGAGCGCACT GCCTTTCCTG AAGCCCATCC ATGCGAGAGT TCCGATTTCC 
CCTGGCCGCC AGCGCCGACA CACGCTCCCA GCAAGCGAGT TTCGCTCGCT CAACGCCGAG 
GACGCAATCA GTGTGTTCGA AATTGAGAGA GAGGCTTTTA TTTCAGTCTC TGGAGAATGT 
CCTCTTCACC TGGATGAAGT CAGGCACTTT CTCACTCTCT GTCCTGAGCT TTCTTTGGGC 
TGGTTTGAAC AGGGAAGGCT TGTCGCTTTT ATCATAGGAT CTCTCTGGGA CCAGGACCGT 
CTGACTGCTG ATGCACTGAC CCTTCATAAA CCTCATGGAA CTACTGTTCA CATCCATGTG 
CTGGCAGTCC ATCGGACCTT CCGCCAGCAA GGAAAAGGAT CCATCCTCTT GTGGCGTTAC 
TTGCAGTATC TGCGCTGTCT ACCTTATGTG CGCCGTGCCG TCCTTATGTG TGAGGATTTC 
TTGGTGCCCT TCTACCAGAA GTCTGGCTTC AAAGGGCAGG GTCCTTCTGA AATCATTGTA 
GGGTCACTTA CCTTCATTGA GATGTATTAT CCTATACGAG GACATGCATT CGTGCGGCGT 
AACAGTGGCT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956998.1
CDS	79..693
Translation	MSVVSALPFLKPIHARVPISPGRQRRHTLPASEFRSLNAEDAISVFEIEREAFISVSGECPLHLDEVRHFLTLCPELSLGWFEQGRLVAFIIGSLWDQDRLTADALTLHKPHGTTVHIHVLAVHRTFRQQGKGSILLWRYLQYLRCLPYVRRAVLMCEDFLVPFYQKSGFKGQGPSEIIVGSLTFIEMYYPIRGHAFVRRNSGC

Target ORF information:

RefSeq Version	NM_200704.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio arylalkylamine N-acetyltransferase 1 (aanat1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200704.1

ORF Insert Sequence:

ATGTCAGTAG TGAGCGCACT GCCTTTCCTG AAGCCCATCC ATGCGAGAGT TCCGATTTCC 
CCTGGCCGCC AGCGCCGACA CACGCTCCCA GCAAGCGAGT TTCGCTCGCT CAACGCCGAG 
GACGCAATCA GTGTGTTCGA AATTGAGAGA GAGGCTTTTA TTTCAGTCTC TGGAGAATGT 
CCTCTTCACC TGGATGAAGT CAGGCACTTT CTCACTCTCT GTCCTGAGCT TTCTTTGGGC 
TGGTTTGAAC AGGGAAGGCT TGTCGCTTTT ATCATAGGAT CTCTCTGGGA CCAGGACCGT 
CTGACTGCTG ATGCACTGAC CCTTCATAAA CCTCATGGAA CTACTGTTCA CATCCATGTG 
CTGGCAGTCC ATCGGACCTT CCGCCAGCAA GGAAAAGGAT CCATCCTCTT GTGGCGTTAC 
TTGCAGTATC TGCGCTGTCT ACCTTATGTG CGCCGTGCCG TCCTTATGTG TGAGGATTTC 
TTGGTGCCCT TCTACCAGAA GTCTGGCTTC AAAGGGCAGG GTCCTTCTGA AATCATTGTA 
GGGTCACTTA CCTTCATTGA GATGTATTAT CCTATACGAG GACATGCATT CGTGCGGCGT 
AACAGTGGCT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular and functional characterization of the voltage-gated proton channel in zebrafish neutrophils.
Physiological reports5(15)(2017 Aug)
Ratanayotha A,Kawai T,Higashijima SI,Okamura Y

Melatonin biosynthesizing enzyme genes and clock genes in ovary and whole brain of zebrafish (Danio rerio): Differential expression and a possible interplay.
General and comparative endocrinology23316-31(2016 Jul)
Khan ZA,Yumnamcha T,Rajiv C,Sanjita Devi H,Mondal G,Devi SD,Bharali R,Chattoraj A