hif1ab cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	hif1ab
Entrez Gene ID	393202
Full Name	hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) b
Synonyms	hif1a,wu:fb63a10,zgc:55405
General protein information	Preferred Names hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) b Names hypoxia-inducible factor 1-alpha HIF1B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001310042.1	NP_001296971.1	hypoxia-inducible factor 1-alpha

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	HIF1A	XP_003639249.1
Homo sapiens (human)	HIF1A	NP_001521.1
Rattus norvegicus (Norway rat)	Hif1a	NP_077335.1
	HIF1A	XP_001099149.1
Bos taurus (cattle)	HIF1A	NP_776764.2
Mus musculus (house mouse)	Hif1a	NP_034561.2
Danio rerio (zebrafish)	hif1ab	NP_956527.1
Pan troglodytes (chimpanzee)	HIF1A	XP_001168972.1
Gallus gallus (chicken)	HIF1A	NP_989628.1

Gene Ontology

The following hif1ab gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hif1ab cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa24773	NM_001310042.1 Latest version!	Danio rerio hypoxia inducible factor 1 subunit alpha b (hif1ab), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hif1ab ( NM_001310042.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001296971.1 Danio rerio hypoxia inducible factor 1 subunit alpha b (hif1ab), mRNA.

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CloneID	ODa24773
Clone ID Related Accession (Same CDS sequence)	NM_001310042.1
Accession Version	NM_001310042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Danio rerio(zebrafish)
Product	hypoxia-inducible factor 1-alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX255914.3, EH457403.1, EH483130.1 and EH458841.1. On Jun 23, 2015 this sequence version replaced XM_005160492.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY326951.1, GFIL01012516.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATACTG GAGTTGTCAC TGAAAAGAAA AGGGTGAGCT CGGAGCGCAG GAAGGAGAAG 
TCCAGGGATG CAGCGCGATC TCGCAGGGGA AAGGAGTCTG AGGTGTTCTA CGAGTTAGCA 
CACCAGCTCC CCCTGCCACA CAATGTCACG TCTCACCTGG ACAAAGCCTC CATTATGAGG 
CTCACCATCA GCTACCTGCG CATGAGGAAG CTGCTCAATT CCGATGAAAA AGAGGAGAAG 
GAGGAGAATG AGCTGGAAAG TCAGCTGAAT GGCTTTTATC TGAAGGCCCT TGAGGGTTTC 
CTTATGGTCC TGTCTGAGGA TGGAGACATG GTTTATCTCT CTGAGAATGT CAGCAAGAGC 
ATGGGCCTCA CACAGTTTGA TCTGACCGGT CACAGCATCT TTGAATTTTC ACACCCATGT 
GACCATGAAG AGTTGAGAGA GATGCTCGTC CACAGAACAG GATCCAAAAA GACCAAGGAA 
CAAAACACAG AGCGTAGCTT CTTCCTGCGG ATGAAGTGCA CACTCACTAG CAGAGGACGC 
ACCGTCAATA TCAAGTCTGC GACGTGGAAG GTTCTTCACT GCGCTGGTCA TGTTCGTGTG 
CACGAGGGCA GCGAGGCTTC AGAAGACTCT GGCTTTAAAG AGCCCCCTGT CACTTACCTT 
GTGCTCATCT GTGAGCCCAT TCCTCATCCC TCGAACATCG AGGTGCCATT GGACAGCAAG 
ACCTTCCTCA GCCGCCACAC TCTGGACATG AAGTTCTCAT ACTGTGATGA AAGGATCACT 
GAGCTGATGG GATATGAGCC AGATGACCTC TTGAACAGAT CAGTCTACGA GTACTATCAC 
GCCCTTGATT CAGATCACCT CACCAAGACA CATCACAACC TGTTTGCAAA GGGCCAGGCC 
ACCACAGGCC AGTACCGCAT GCTGGCTAAG AAAGGTGGTT TTGTGTGGGT TGAGACTCAG 
GCCACTGTAA TCTACAACCC CAAGAATTCT CAGCCGCAAT GCATTGTGTG CGTCAACTAC 
GTTCTCAGTG GCATTGTAGA GGGGGATGTA GTCCTGTCCT TGCAGCAGAC CGTGACTGAG 
CCCAAGGCTG TTGAGAAAGA AAGTGAGGAG ACTGAGGAAA AGACCTCTGA ATTGGATATT 
CTCAAGCTCT TCAAGCCAGA AAGCCTCAAT TGCTCATTGG AAAGCTCTAC ACTTTATAAT 
AAGCTGAAAG AGGAGCCAGA GGCCCTCACT GTGTTGGCAC CTGCAGCAGG CGACGCCATT 
ATCTCTCTGG ACTTCAACAA CTCAGATTCT GACATACAGC TGCTGAAGGA GGTGCCCCTC 
TACAATGATG TCATGCTGCC TTCCAGCAGT GAGAAGCTGC CACTCAGCCT ATCTCCTCTC 
ACACCCAGCG ACTCCATCCC AGCTCTGACC AAACTAGAGA CTGGAGGAGA GGACTTCCCT 
TTCAGCTCTG CCTCTGATCG TGTGCCAGAC CCCACAAACA CACCCTCCAC ATCTGGACTC 
GGCTCTTCGG GGCCCAACAG CCCCATGGAT TACGGTTTCC CAGTGGAACC AGACATCAGT 
TCTGAATTTA AACTCGACCT GGTTGAGAAA CTGTTTGCTA TTGATACCGA AGCAAAGACA 
CCTTTTTCCA CCCAGCCCAT GGAGGATCTC GACCTAGAGA TGCTGGCTCC TTACATCCCA 
ATGGATGACG ACTTCCAGCT GCGCATCCCA TCTCCACTGG ATCCGCTCCC ATCTGCCACT 
CACTCTGTGT CAGCCATGAG CTCTTTATTC CAACCCTTAC CCTCCTCGCC AGCATCTCCA 
GCCTCATCTA CCAGCAGCAC AGTGAAGCAG GAGGCGTCAT CCCGGGCCCC TTCACCCCTA 
CACCTGCTGC AGGAGGTGTG CAGTGCACCT GTCTCGCCCT TCAGTGGCAG TCGGGATGCC 
TCACCTGTTC GATCCAGCAC CCCACAGAGC AGCAGTCAGC TCAACAACAG AGAAATGTCT 
CCAAAGATGT TAGCCTTCCA AAATATCCAG CGTAAGAGGA AGCTAAACGA AGTGACGTCG 
CTTTCTGAAG CTGTTGGACT GGGGGCTTTG CTTCACAGTG TGGACAGTGC TATAGACCCT 
GGAAAGAGAG CGAAGGTTTT AGAGGTGAAA GGGTCCAGTG TGCTCGGGGG AAACAAAACA 
ATTCTCATAC TGCCCTCTGA TGTGGCCAGT CGTCTGTTGA GCAGTTCTCT AGAGGGCAGC 
GGCGGCCTCC CTCAGCTCAC ACGCTACGAC TGCGAAGTCA ACGCTCCCGT GCAGGACCGC 
CACCATCTGC TCCAGGGAGA GGAGCTGCTG CGTGCTCTGG ACCAAGTCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001296971.1
CDS	389..2722
Translation	MDTGVVTEKKRVSSERRKEKSRDAARSRRGKESEVFYELAHQLPLPHNVTSHLDKASIMRLTISYLRMRKLLNSDEKEEKEENELESQLNGFYLKALEGFLMVLSEDGDMVYLSENVSKSMGLTQFDLTGHSIFEFSHPCDHEELREMLVHRTGSKKTKEQNTERSFFLRMKCTLTSRGRTVNIKSATWKVLHCAGHVRVHEGSEASEDSGFKEPPVTYLVLICEPIPHPSNIEVPLDSKTFLSRHTLDMKFSYCDERITELMGYEPDDLLNRSVYEYYHALDSDHLTKTHHNLFAKGQATTGQYRMLAKKGGFVWVETQATVIYNPKNSQPQCIVCVNYVLSGIVEGDVVLSLQQTVTEPKAVEKESEETEEKTSELDILKLFKPESLNCSLESSTLYNKLKEEPEALTVLAPAAGDAIISLDFNNSDSDIQLLKEVPLYNDVMLPSSSEKLPLSLSPLTPSDSIPALTKLETGGEDFPFSSASDRVPDPTNTPSTSGLGSSGPNSPMDYGFPVEPDISSEFKLDLVEKLFAIDTEAKTPFSTQPMEDLDLEMLAPYIPMDDDFQLRIPSPLDPLPSATHSVSAMSSLFQPLPSSPASPASSTSSTVKQEASSRAPSPLHLLQEVCSAPVSPFSGSRDASPVRSSTPQSSSQLNNREMSPKMLAFQNIQRKRKLNEVTSLSEAVGLGALLHSVDSAIDPGKRAKVLEVKGSSVLGGNKTILILPSDVASRLLSSSLEGSGGLPQLTRYDCEVNAPVQDRHHLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	NM_001310042.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio hypoxia inducible factor 1 subunit alpha b (hif1ab), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310042.1

ORF Insert Sequence:

ATGGATACTG GAGTTGTCAC TGAAAAGAAA AGGGTGAGCT CGGAGCGCAG GAAGGAGAAG 
TCCAGGGATG CAGCGCGATC TCGCAGGGGA AAGGAGTCTG AGGTGTTCTA CGAGTTAGCA 
CACCAGCTCC CCCTGCCACA CAATGTCACG TCTCACCTGG ACAAAGCCTC CATTATGAGG 
CTCACCATCA GCTACCTGCG CATGAGGAAG CTGCTCAATT CCGATGAAAA AGAGGAGAAG 
GAGGAGAATG AGCTGGAAAG TCAGCTGAAT GGCTTTTATC TGAAGGCCCT TGAGGGTTTC 
CTTATGGTCC TGTCTGAGGA TGGAGACATG GTTTATCTCT CTGAGAATGT CAGCAAGAGC 
ATGGGCCTCA CACAGTTTGA TCTGACCGGT CACAGCATCT TTGAATTTTC ACACCCATGT 
GACCATGAAG AGTTGAGAGA GATGCTCGTC CACAGAACAG GATCCAAAAA GACCAAGGAA 
CAAAACACAG AGCGTAGCTT CTTCCTGCGG ATGAAGTGCA CACTCACTAG CAGAGGACGC 
ACCGTCAATA TCAAGTCTGC GACGTGGAAG GTTCTTCACT GCGCTGGTCA TGTTCGTGTG 
CACGAGGGCA GCGAGGCTTC AGAAGACTCT GGCTTTAAAG AGCCCCCTGT CACTTACCTT 
GTGCTCATCT GTGAGCCCAT TCCTCATCCC TCGAACATCG AGGTGCCATT GGACAGCAAG 
ACCTTCCTCA GCCGCCACAC TCTGGACATG AAGTTCTCAT ACTGTGATGA AAGGATCACT 
GAGCTGATGG GATATGAGCC AGATGACCTC TTGAACAGAT CAGTCTACGA GTACTATCAC 
GCCCTTGATT CAGATCACCT CACCAAGACA CATCACAACC TGTTTGCAAA GGGCCAGGCC 
ACCACAGGCC AGTACCGCAT GCTGGCTAAG AAAGGTGGTT TTGTGTGGGT TGAGACTCAG 
GCCACTGTAA TCTACAACCC CAAGAATTCT CAGCCGCAAT GCATTGTGTG CGTCAACTAC 
GTTCTCAGTG GCATTGTAGA GGGGGATGTA GTCCTGTCCT TGCAGCAGAC CGTGACTGAG 
CCCAAGGCTG TTGAGAAAGA AAGTGAGGAG ACTGAGGAAA AGACCTCTGA ATTGGATATT 
CTCAAGCTCT TCAAGCCAGA AAGCCTCAAT TGCTCATTGG AAAGCTCTAC ACTTTATAAT 
AAGCTGAAAG AGGAGCCAGA GGCCCTCACT GTGTTGGCAC CTGCAGCAGG CGACGCCATT 
ATCTCTCTGG ACTTCAACAA CTCAGATTCT GACATACAGC TGCTGAAGGA GGTGCCCCTC 
TACAATGATG TCATGCTGCC TTCCAGCAGT GAGAAGCTGC CACTCAGCCT ATCTCCTCTC 
ACACCCAGCG ACTCCATCCC AGCTCTGACC AAACTAGAGA CTGGAGGAGA GGACTTCCCT 
TTCAGCTCTG CCTCTGATCG TGTGCCAGAC CCCACAAACA CACCCTCCAC ATCTGGACTC 
GGCTCTTCGG GGCCCAACAG CCCCATGGAT TACGGTTTCC CAGTGGAACC AGACATCAGT 
TCTGAATTTA AACTCGACCT GGTTGAGAAA CTGTTTGCTA TTGATACCGA AGCAAAGACA 
CCTTTTTCCA CCCAGCCCAT GGAGGATCTC GACCTAGAGA TGCTGGCTCC TTACATCCCA 
ATGGATGACG ACTTCCAGCT GCGCATCCCA TCTCCACTGG ATCCGCTCCC ATCTGCCACT 
CACTCTGTGT CAGCCATGAG CTCTTTATTC CAACCCTTAC CCTCCTCGCC AGCATCTCCA 
GCCTCATCTA CCAGCAGCAC AGTGAAGCAG GAGGCGTCAT CCCGGGCCCC TTCACCCCTA 
CACCTGCTGC AGGAGGTGTG CAGTGCACCT GTCTCGCCCT TCAGTGGCAG TCGGGATGCC 
TCACCTGTTC GATCCAGCAC CCCACAGAGC AGCAGTCAGC TCAACAACAG AGAAATGTCT 
CCAAAGATGT TAGCCTTCCA AAATATCCAG CGTAAGAGGA AGCTAAACGA AGTGACGTCG 
CTTTCTGAAG CTGTTGGACT GGGGGCTTTG CTTCACAGTG TGGACAGTGC TATAGACCCT 
GGAAAGAGAG CGAAGGTTTT AGAGGTGAAA GGGTCCAGTG TGCTCGGGGG AAACAAAACA 
ATTCTCATAC TGCCCTCTGA TGTGGCCAGT CGTCTGTTGA GCAGTTCTCT AGAGGGCAGC 
GGCGGCCTCC CTCAGCTCAC ACGCTACGAC TGCGAAGTCA ACGCTCCCGT GCAGGACCGC 
CACCATCTGC TCCAGGGAGA GGAGCTGCTG CGTGCTCTGG ACCAAGTCAA CTGA