nsfa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nsfa
Entrez Gene ID	368483
Full Name	N-ethylmaleimide-sensitive factor a
Synonyms	nsf,si:bz18k17.1,wu:fj33g11
General protein information	Preferred Names N-ethylmaleimide-sensitive factor a Names vesicle-fusing ATPase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001044328.1	NP_001037793.1	vesicle-fusing ATPase
XM_021469743.1	XP_021325418.1	vesicle-fusing ATPase isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NSF	XP_511626.3
Canis lupus familiaris (dog)	NSF	XP_548044.2
Mus musculus (house mouse)	Nsf	NP_032766.2
Drosophila melanogaster (fruit fly)	comt	NP_001259506.1
Saccharomyces cerevisiae (baker's yeast)	SEC18	NP_009636.3
Gallus gallus (chicken)	NSF	NP_001019627.1
Danio rerio (zebrafish)	nsfa	NP_001037793.1
Neurospora crassa	NCU03387	XP_956834.1
Xenopus tropicalis (tropical clawed frog)	nsf	NP_001072788.1
Bos taurus (cattle)	NSF	XP_002696103.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012684	XP_307148.3
Schizosaccharomyces pombe (fission yeast)	sec18	NP_594690.1
Magnaporthe oryzae (rice blast fungus)	MGG_02418	XP_003709204.1
	NSF	XP_002800596.1
Caenorhabditis elegans (roundworm)	nsf-1	NP_001076603.1
Caenorhabditis elegans (roundworm)	nsf-1	NP_001076604.1
Homo sapiens (human)	NSF	NP_006169.2
Rattus norvegicus (Norway rat)	Nsf	NP_068516.1
Drosophila melanogaster (fruit fly)	Nsf2	NP_788676.1
Kluyveromyces lactis	KLLA0C14520g	XP_452850.1
Eremothecium gossypii	AGOS_AER169C	NP_985027.1

Gene Ontology
Bibliography

Related articles in PubMed

Modelling acrylamide acute neurotoxicity in zebrafish larvae.
Prats E, G?mez-Canela C, Ben-Lulu S, Ziv T, Padr?s F, Tornero D, Garcia-Reyero N, Tauler R, Admon A, Rald?a D
Scientific reports7(1)13952(2017 Oct)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Neuronal Ndrg4 Is Essential for Nodes of Ranvier Organization in Zebrafish.
Fontenas L, De Santis F, Di Donato V, Degerny C, Chambraud B, Del Bene F, Tawk M
PLoS genetics12(11)e1006459(2016 Nov)

Maternal Rest/Nrsf Regulates Zebrafish Behavior through snap25a/b.
Moravec CE, Samuel J, Weng W, Wood IC, Sirotkin HI
The Journal of neuroscience : the official journal of the Society for Neuroscience36(36)9407-19(2016 09)

N-Ethylmaleimide-Sensitive Factor b (nsfb) Is Required for Normal Pigmentation of the Zebrafish Retinal Pigment Epithelium.
Hanovice NJ, Daly CM, Gross JM
Investigative ophthalmology & visual science56(12)7535-44(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Molecular analysis of ndrg4 mutants shows that expression of snap25 and nsf are sharply decreased, revealing a role of ndrg4 in controlling vesicle exocytosis. This uncovers a previously unknown function of ndrg4 in regulating vesicle docking and nodes of Ranvier organization, at least through its ability to finely tune the expression of the t-SNARE/NSF machinery.
Title: Neuronal Ndrg4 Is Essential for Nodes of Ranvier Organization in Zebrafish.

results establish a role for Nsf in the maintenance of synaptic contacts between hair cells and afferent neurons, mediated in part via the secretion of trophic signaling factors
Title: Both pre- and postsynaptic activity of Nsf prevents degeneration of hair-cell synapses.

This study analyses of nsf mutant zebrafish reveal an unexpected role for NSF in hypothalamic development, with mutant 5 days post-fertilization larvae exhibiting a stage-dependent loss of neuroendocrine transcripts.
Title: Neuroendocrine transcriptional programs adapt dynamically to the supply and demand for neuropeptides as revealed in NSF mutant zebrafish.

Activation of PKCgamma is necessary for the activity-dependent trafficking of AMPARs in embryonic zebrafish and is NMDA and SNARE-dependent and requires AMPARs to associate with both NSF and PICK1.
Title: PKCgamma-induced trafficking of AMPA receptors in embryonic zebrafish depends on NSF and PICK1.

Nsf is essential in the formation of the nodes of Ranvier in the vertebrate nervous system.
Title: nsf is essential for organization of myelinated axons in zebrafish.

The following nsfa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nsfa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00986	NM_001044328.1 Latest version!	Danio rerio N-ethylmaleimide-sensitive factor a (nsfa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa08429	XM_021469743.1 Latest version!	Danio rerio N-ethylmaleimide-sensitive factor a (nsfa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nsfa ( NM_001044328.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001037793.1 Danio rerio N-ethylmaleimide-sensitive factor a (nsfa), mRNA.

Price & Availability↑

CloneID	ODa00986
Clone ID Related Accession (Same CDS sequence)	NM_001044328.1
Accession Version	NM_001044328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	vesicle-fusing ATPase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL590145.3. On or before Apr 15, 2008 this sequence version replaced NM_201490.1, XM_697421.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050490.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGACCC GGACGATGCA AGCAGCCCGA TGCCCGACAG ATGAGCTGTC ACTGACTAAC 
TGTGCTGTAG TGAGTGAGAA AGACCTGCAG TCTGGACAGC ATGTGACTGT GAGGACAACA 
CCCACCCACA AGTTTGTGTT CACAGTGAAG GGTCACCACT CTGTGCTCCC TGGAACCATC 
GCTTTCAGTT TGCCACAGAG GAAATGGGCA GGCCTGTCCA TTGGGCAAGA AATAGAAGTG 
TCAAACTACA ACTTTGACAA GTCCAGGCAG TGCATTGGTG CCATGACCAT TGAGATTGAC 
TTCCTGCAGA AGAAGAGCAC AGACTCCAGC CCTTACGACT CAGACAAAAT GGCTGCTGAG 
TTCATCCAGC ATTTTAACAA TCAGAGCTTC TCCGTGGGGC AGCAGCTGGT CTTCAGTTTT 
TGTGACAAGC TCTTCGGCCT ACTTATCAAA GATATTGAGG CGATGGATTC CAGCATTCTG 
AAGGGTGAAC CAGCATCTGG CAAAAAGCAA AAGATTGAGG TCGGCCTGTT GGTTGGAAAC 
AGTCAAGTGA TTTTTGAAAA ATCAGAGAGC TCATCTCTCA CTCTAGTTGG CAAGGCTAAA 
ACTAAAGAAG CCCGTCAAAC CATCATCAAC CCTGACTGGA ACTTTGAGAA AATGGGCATT 
GGTGGTTTAG ACAAGGAGTT CTCTGATATC TTCCGCAGGG CCTTTGCCTC TCGAGTCTTT 
CCTCCAGACA TTGTGGAGCA GATGGGTTGT AAGCATGTGA AAGGTATCCT GCTCTTTGGT 
CCTCCTGGCT GTGGTAAAAC ACTGATGGCC AGGCAGATCG GTAAAATGCT AAATGCCAGA 
GAGCCAAAGA TAGTCAACGG ACCAGAGATC CTCAACAAGT ACGTGGGAGA GTCTGAGGCC 
AACATTAGGA AACTGTTCGC GGATGCTGAG GAGGAACAGA AAAGGTTGGG TGCTAACAGC 
GGTCTGCACA TCATCATCTT TGATGAACTG GATGCCATCT GTAAGCAGCG TGGTACAGGC 
GCCAGCAGCA CAGGTGTGCA CGACACTGTG GTTAACCAGC TGCTGTCCAA GATCGACGGA 
GTGGAGCAGC TCAACAACAT CCTCGTCATC GGCATGACTA ATAGGCCTGA CCTGATAGAT 
GAAGCCTTGA TGAGACCTGG CCGATTTGAA GTAAAGATGG AAATCGGTTT GCCAGATGAG 
AAGGGTCGTG TTCAGATCCT GAACATCCAC ACAGCTAAGA TGCGAGAGTT TAAACTCCTG 
GCTTCTGATG TGGATGTGAA AGAGTTGGCT GCTGAGACCA AGAACTACAG TGGAGCTGAA 
CTGGAGGGTC TGGTCAGAGC TGCACAATCC ACTGCCATGA ACCGTCACAT TAAGGCAACA 
TCTACCGTAG AGGTGGACAT GGAGCGAGCA GAAAAGCTAC AGGTCACTCG AACAGATTTC 
ATGGCGTCCT TGAATAACGA CATTAAACCT GCTTTTGGCA CCAACCAAGA GGACTATTCC 
AGCTACATCA TGAACGGCAT CATTAAGTGG GGAGATCCAG TGACCCGAGT GCTGGAGGAC 
GGAGAACTGC TGGTGCAGCA GACCAAGAAC AGCGACCGCA CTCCGCTGGT GTCTGTGCTT 
CTGGAGGGAC CTCCTCACAG TGGAAAGACA GCTCTGGCTG CCAAAATCTC AGAGGACTCG 
CAGTTCCCAT TCATTAAGAT CTGCTCTCCA GACAAGATGA TCGGCCACTC AGAGATCTCC 
AAATGCCAGG CAATCAAGAA GATCTTCGAT GATGCCTACA AGTCTCAGCT CAGCTGTGTC 
GTGGTTGATG ACATTGAGCG CCTCTTAGAT TACGTGCCGA TCGGCCCTCG ATTCTCCAAC 
CTGGTGCTTC AAGCCCTGCT GGTCTTACTG AAGAAGACTC CACCTCATGG TCGTAAGCTG 
CTTATCATTG GCACCACCAG CCGTAAAGAC GTCCTGCAGG AGATGGAGAT GCTGGATGCG 
TTCAGCACCA CCATCCACGT GCAGAATATC TCAAGTGGGG ATCATCTGGT TGAAGCTCTA 
GAGCTCCTGG GGAGCTTCAC AGATGCTGAG AGAACCACCA TCGCCCAGCA TGTGAAAGGA 
AAAAGAGTGT GGATTGGCAT TAAGAAGCTG CTCATGCTTA TTGAAATGTC TCTGCAGATG 
GATCAGGAGT ACAGAGTTAG CAAGTTCCTT TCTCTACTCA AAGATGAGGG AGCAGACCGC 
GGCTTCTTCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001037793.1
CDS	66..2300
Translation	MATRTMQAARCPTDELSLTNCAVVSEKDLQSGQHVTVRTTPTHKFVFTVKGHHSVLPGTIAFSLPQRKWAGLSIGQEIEVSNYNFDKSRQCIGAMTIEIDFLQKKSTDSSPYDSDKMAAEFIQHFNNQSFSVGQQLVFSFCDKLFGLLIKDIEAMDSSILKGEPASGKKQKIEVGLLVGNSQVIFEKSESSSLTLVGKAKTKEARQTIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLFGPPGCGKTLMARQIGKMLNAREPKIVNGPEILNKYVGESEANIRKLFADAEEEQKRLGANSGLHIIIFDELDAICKQRGTGASSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALMRPGRFEVKMEIGLPDEKGRVQILNIHTAKMREFKLLASDVDVKELAAETKNYSGAELEGLVRAAQSTAMNRHIKATSTVEVDMERAEKLQVTRTDFMASLNNDIKPAFGTNQEDYSSYIMNGIIKWGDPVTRVLEDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKISEDSQFPFIKICSPDKMIGHSEISKCQAIKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKTPPHGRKLLIIGTTSRKDVLQEMEMLDAFSTTIHVQNISSGDHLVEALELLGSFTDAERTTIAQHVKGKRVWIGIKKLLMLIEMSLQMDQEYRVSKFLSLLKDEGADRGFFE

Target ORF information:

RefSeq Version	NM_001044328.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio N-ethylmaleimide-sensitive factor a (nsfa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044328.1

ORF Insert Sequence:

ATGGCGACCC GGACGATGCA AGCAGCCCGA TGCCCGACAG ATGAGCTGTC ACTGACTAAC 
TGTGCTGTAG TGAGTGAGAA AGACCTGCAG TCTGGACAGC ATGTGACTGT GAGGACAACA 
CCCACCCACA AGTTTGTGTT CACAGTGAAG GGTCACCACT CTGTGCTCCC TGGAACCATC 
GCTTTCAGTT TGCCACAGAG GAAATGGGCA GGCCTGTCCA TTGGGCAAGA AATAGAAGTG 
TCAAACTACA ACTTTGACAA GTCCAGGCAG TGCATTGGTG CCATGACCAT TGAGATTGAC 
TTCCTGCAGA AGAAGAGCAC AGACTCCAGC CCTTACGACT CAGACAAAAT GGCTGCTGAG 
TTCATCCAGC ATTTTAACAA TCAGAGCTTC TCCGTGGGGC AGCAGCTGGT CTTCAGTTTT 
TGTGACAAGC TCTTCGGCCT ACTTATCAAA GATATTGAGG CGATGGATTC CAGCATTCTG 
AAGGGTGAAC CAGCATCTGG CAAAAAGCAA AAGATTGAGG TCGGCCTGTT GGTTGGAAAC 
AGTCAAGTGA TTTTTGAAAA ATCAGAGAGC TCATCTCTCA CTCTAGTTGG CAAGGCTAAA 
ACTAAAGAAG CCCGTCAAAC CATCATCAAC CCTGACTGGA ACTTTGAGAA AATGGGCATT 
GGTGGTTTAG ACAAGGAGTT CTCTGATATC TTCCGCAGGG CCTTTGCCTC TCGAGTCTTT 
CCTCCAGACA TTGTGGAGCA GATGGGTTGT AAGCATGTGA AAGGTATCCT GCTCTTTGGT 
CCTCCTGGCT GTGGTAAAAC ACTGATGGCC AGGCAGATCG GTAAAATGCT AAATGCCAGA 
GAGCCAAAGA TAGTCAACGG ACCAGAGATC CTCAACAAGT ACGTGGGAGA GTCTGAGGCC 
AACATTAGGA AACTGTTCGC GGATGCTGAG GAGGAACAGA AAAGGTTGGG TGCTAACAGC 
GGTCTGCACA TCATCATCTT TGATGAACTG GATGCCATCT GTAAGCAGCG TGGTACAGGC 
GCCAGCAGCA CAGGTGTGCA CGACACTGTG GTTAACCAGC TGCTGTCCAA GATCGACGGA 
GTGGAGCAGC TCAACAACAT CCTCGTCATC GGCATGACTA ATAGGCCTGA CCTGATAGAT 
GAAGCCTTGA TGAGACCTGG CCGATTTGAA GTAAAGATGG AAATCGGTTT GCCAGATGAG 
AAGGGTCGTG TTCAGATCCT GAACATCCAC ACAGCTAAGA TGCGAGAGTT TAAACTCCTG 
GCTTCTGATG TGGATGTGAA AGAGTTGGCT GCTGAGACCA AGAACTACAG TGGAGCTGAA 
CTGGAGGGTC TGGTCAGAGC TGCACAATCC ACTGCCATGA ACCGTCACAT TAAGGCAACA 
TCTACCGTAG AGGTGGACAT GGAGCGAGCA GAAAAGCTAC AGGTCACTCG AACAGATTTC 
ATGGCGTCCT TGAATAACGA CATTAAACCT GCTTTTGGCA CCAACCAAGA GGACTATTCC 
AGCTACATCA TGAACGGCAT CATTAAGTGG GGAGATCCAG TGACCCGAGT GCTGGAGGAC 
GGAGAACTGC TGGTGCAGCA GACCAAGAAC AGCGACCGCA CTCCGCTGGT GTCTGTGCTT 
CTGGAGGGAC CTCCTCACAG TGGAAAGACA GCTCTGGCTG CCAAAATCTC AGAGGACTCG 
CAGTTCCCAT TCATTAAGAT CTGCTCTCCA GACAAGATGA TCGGCCACTC AGAGATCTCC 
AAATGCCAGG CAATCAAGAA GATCTTCGAT GATGCCTACA AGTCTCAGCT CAGCTGTGTC 
GTGGTTGATG ACATTGAGCG CCTCTTAGAT TACGTGCCGA TCGGCCCTCG ATTCTCCAAC 
CTGGTGCTTC AAGCCCTGCT GGTCTTACTG AAGAAGACTC CACCTCATGG TCGTAAGCTG 
CTTATCATTG GCACCACCAG CCGTAAAGAC GTCCTGCAGG AGATGGAGAT GCTGGATGCG 
TTCAGCACCA CCATCCACGT GCAGAATATC TCAAGTGGGG ATCATCTGGT TGAAGCTCTA 
GAGCTCCTGG GGAGCTTCAC AGATGCTGAG AGAACCACCA TCGCCCAGCA TGTGAAAGGA 
AAAAGAGTGT GGATTGGCAT TAAGAAGCTG CTCATGCTTA TTGAAATGTC TCTGCAGATG 
GATCAGGAGT ACAGAGTTAG CAAGTTCCTT TCTCTACTCA AAGATGAGGG AGCAGACCGC 
GGCTTCTTCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsfa ( XM_021469743.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325418.1 Danio rerio N-ethylmaleimide-sensitive factor a (nsfa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08429
Clone ID Related Accession (Same CDS sequence)	XM_021469743.1
Accession Version	XM_021469743.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	vesicle-fusing ATPase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCC GGACGATGCA AGCAGCCCGA TGCCCGACAG ATGAGCTGTC ACTGACTAAC 
TGTGCTGTAG TGAGTGAGAA AGACCTGCAG TCTGGACAGC ATGTGACTGT GAGGACAACA 
CCCACCCACA AGTTTGTGTT CACAGTGAAG GGTCACCACT CTGTGCTCCC TGGAACCATC 
GCTTTCAGTT TGCCACAGAG GAAATGGGCA GGCCTGTCCA TTGGGCAAGA AATAGAAGTG 
TCAAACTACA ACTTTGACAA GTCCAGGCAG TGCATTGGTG CCATGACCAT TGAGATTGAC 
TTCCTGCAGA AGAAGAGCAC AGACTCCAGC CCTTACGACT CAGACAAAAT GGCTGCTGAG 
TTCATCCAGC ATTTTAACAA TCAGAGCTTC TCCGTGGGGC AGCAGCTGGT CTTCAGTTTT 
TGTGACAAGC TCTTCGGCCT ACTTATCAAA GATATTGAGG CGATGGATTC CAGCATTCTG 
AAGGGTGAAC CAGCATCTGG CAAAAAGCAA AAGATTGAGG TCGGCCTGTT GGTTGGAAAC 
AGTCAAGTGA TTTTTGAAAA ATCAGAGAGC TCATCTCTCA CTCTAGTTGG CAAGGCTAAA 
ACTAAAGAAG CCCGTCAAAC CATCATCAAC CCTGACTGGA ACTTTGAGAA AATGGGCATT 
GGTGGTTTAG ACAAGGAGTT CTCTGATATC TTCCGCAGGG CCTTTGCCTC TCGAGTCTTT 
CCTCCAGACA TTGTGGAGCA GATGGGTTGT AAGCATGTGA AAGGTATCCT GCTCTTTGGT 
CCTCCTGGCT GTGGTAAAAC ACTGATGGCC AGGCAGATCG GTAAAATGCT AAATGCCAGA 
GAGCCAAAGA TAGTCAACGG ACCAGAGATC CTCAACAAGT ACGTGGGAGA GTCTGAGGCC 
AACATTAGGA AACTGTTCGC GGATGCTGAG GAGGAACAGA AAAGGTTGGG TGCTAACAGC 
GGTCTGCACA TCATCATCTT TGATGAACTG GATGCCATCT GTAAGCAGCG TGGTACAGGC 
GCCAGCAGCA CAGGTGTGCA CGACACTGTG GTTAACCAGC TGCTGTCCAA GATCGACGGA 
GTGGAGCAGC TCAACAACAT CCTCGTCATC GGCATGACTA ATAGGCCTGA CCTGATAGAT 
GAAGCCTTGA TGAGACCTGG CCGATTTGAA GTAAAGATGG AAATCGGTTT GCCAGATGAG 
AAGGGTCGTG TTCAGATCCT GAACATCCAC ACAGCTAAGA TGCGAGAGTT TAAACTCCTG 
GCTTCTGATG TGGATGTGAA AGAGTTGGCT GCTGAGACCA AGAACTACAG TGGAGCTGAA 
CTGGAGGGTC TGGTCAGAGC TGCACAATCC ACTGCCATGA ACCGTCACAT TAAGGCAACA 
TCTACCGTAG AGGTGGACAT GGAGCGAGCA GAAAAGCTAC AGGTCACTCG AACAGATTTC 
ATGGCGTCCT TGAATAACGA CATTAAACCT GCTTTTGGCA CCAACCAAGA GGACTATTCC 
AGCTACATCA TGAACGGCAT CATTAAGTGG GGAGATCCAG TGACCCGAGT GCTGGAGGAC 
GGAGAACTGC TGGTGCAGCA GACCAAGAAC AGCGACCGCA CTCCGCTGGT GTCTGTGCTT 
CTGGAGGGAC CTCCTCACAG TGGAAAGACA GCTCTGGCTG CCAAAATCTC AGAGGACTCG 
CAGTTCCCAT TCATTAAGAT CTGCTCTCCA GACAAGATGA TCGGCCACTC AGAGATCTCC 
AAATGCCAGG CAATCAAGAA GATCTTCGAT GATGCCTACA AGTCTCAGCT CAGCTGTGTC 
GTGGTTGATG ACATTGAGCG CCTCTTAGAT TACGTGCCGA TCGGCCCTCG ATTCTCCAAC 
CTGGTGCTTC AAGCCCTGCT GGTCTTACTG AAGAAGACTC CACCTCATGG TCGTAAGCTG 
CTTATCATTG GCACCACCAG CCGTAAAGAC GTCCTGCAGG AGATGGAGAT GCTGGATGCG 
TTCAGCACCA CCATCCACGT GCAGAATATC TCAAGTGGGG ATCATCTGGT TGAAGCTCTA 
GAGCTCCTGG GGAGCTTCAC AGATGCTGAG AGAACCACCA TCGCCCAGCA TGTGAAAGGA 
AAAAGAGTGT GGATTGGCAT TAAGAAGCTG CTCATGCTTA TTGAAATGTC TCTGCAGATG 
GATCAGGAGT ACAGAGTTAG CAAGTTCCTT TCTCTACTCA AAGATGAGGG AGCGCTGGAT 
GAAAACAATC ACATTCGAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325418.1
CDS	101..2344
Translation	MATRTMQAARCPTDELSLTNCAVVSEKDLQSGQHVTVRTTPTHKFVFTVKGHHSVLPGTIAFSLPQRKWAGLSIGQEIEVSNYNFDKSRQCIGAMTIEIDFLQKKSTDSSPYDSDKMAAEFIQHFNNQSFSVGQQLVFSFCDKLFGLLIKDIEAMDSSILKGEPASGKKQKIEVGLLVGNSQVIFEKSESSSLTLVGKAKTKEARQTIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLFGPPGCGKTLMARQIGKMLNAREPKIVNGPEILNKYVGESEANIRKLFADAEEEQKRLGANSGLHIIIFDELDAICKQRGTGASSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALMRPGRFEVKMEIGLPDEKGRVQILNIHTAKMREFKLLASDVDVKELAAETKNYSGAELEGLVRAAQSTAMNRHIKATSTVEVDMERAEKLQVTRTDFMASLNNDIKPAFGTNQEDYSSYIMNGIIKWGDPVTRVLEDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKISEDSQFPFIKICSPDKMIGHSEISKCQAIKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKTPPHGRKLLIIGTTSRKDVLQEMEMLDAFSTTIHVQNISSGDHLVEALELLGSFTDAERTTIAQHVKGKRVWIGIKKLLMLIEMSLQMDQEYRVSKFLSLLKDEGALDENNHIRI

Target ORF information:

RefSeq Version	XM_021469743.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio N-ethylmaleimide-sensitive factor a (nsfa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469743.1

ORF Insert Sequence:

ATGGCGACCC GGACGATGCA AGCAGCCCGA TGCCCGACAG ATGAGCTGTC ACTGACTAAC 
TGTGCTGTAG TGAGTGAGAA AGACCTGCAG TCTGGACAGC ATGTGACTGT GAGGACAACA 
CCCACCCACA AGTTTGTGTT CACAGTGAAG GGTCACCACT CTGTGCTCCC TGGAACCATC 
GCTTTCAGTT TGCCACAGAG GAAATGGGCA GGCCTGTCCA TTGGGCAAGA AATAGAAGTG 
TCAAACTACA ACTTTGACAA GTCCAGGCAG TGCATTGGTG CCATGACCAT TGAGATTGAC 
TTCCTGCAGA AGAAGAGCAC AGACTCCAGC CCTTACGACT CAGACAAAAT GGCTGCTGAG 
TTCATCCAGC ATTTTAACAA TCAGAGCTTC TCCGTGGGGC AGCAGCTGGT CTTCAGTTTT 
TGTGACAAGC TCTTCGGCCT ACTTATCAAA GATATTGAGG CGATGGATTC CAGCATTCTG 
AAGGGTGAAC CAGCATCTGG CAAAAAGCAA AAGATTGAGG TCGGCCTGTT GGTTGGAAAC 
AGTCAAGTGA TTTTTGAAAA ATCAGAGAGC TCATCTCTCA CTCTAGTTGG CAAGGCTAAA 
ACTAAAGAAG CCCGTCAAAC CATCATCAAC CCTGACTGGA ACTTTGAGAA AATGGGCATT 
GGTGGTTTAG ACAAGGAGTT CTCTGATATC TTCCGCAGGG CCTTTGCCTC TCGAGTCTTT 
CCTCCAGACA TTGTGGAGCA GATGGGTTGT AAGCATGTGA AAGGTATCCT GCTCTTTGGT 
CCTCCTGGCT GTGGTAAAAC ACTGATGGCC AGGCAGATCG GTAAAATGCT AAATGCCAGA 
GAGCCAAAGA TAGTCAACGG ACCAGAGATC CTCAACAAGT ACGTGGGAGA GTCTGAGGCC 
AACATTAGGA AACTGTTCGC GGATGCTGAG GAGGAACAGA AAAGGTTGGG TGCTAACAGC 
GGTCTGCACA TCATCATCTT TGATGAACTG GATGCCATCT GTAAGCAGCG TGGTACAGGC 
GCCAGCAGCA CAGGTGTGCA CGACACTGTG GTTAACCAGC TGCTGTCCAA GATCGACGGA 
GTGGAGCAGC TCAACAACAT CCTCGTCATC GGCATGACTA ATAGGCCTGA CCTGATAGAT 
GAAGCCTTGA TGAGACCTGG CCGATTTGAA GTAAAGATGG AAATCGGTTT GCCAGATGAG 
AAGGGTCGTG TTCAGATCCT GAACATCCAC ACAGCTAAGA TGCGAGAGTT TAAACTCCTG 
GCTTCTGATG TGGATGTGAA AGAGTTGGCT GCTGAGACCA AGAACTACAG TGGAGCTGAA 
CTGGAGGGTC TGGTCAGAGC TGCACAATCC ACTGCCATGA ACCGTCACAT TAAGGCAACA 
TCTACCGTAG AGGTGGACAT GGAGCGAGCA GAAAAGCTAC AGGTCACTCG AACAGATTTC 
ATGGCGTCCT TGAATAACGA CATTAAACCT GCTTTTGGCA CCAACCAAGA GGACTATTCC 
AGCTACATCA TGAACGGCAT CATTAAGTGG GGAGATCCAG TGACCCGAGT GCTGGAGGAC 
GGAGAACTGC TGGTGCAGCA GACCAAGAAC AGCGACCGCA CTCCGCTGGT GTCTGTGCTT 
CTGGAGGGAC CTCCTCACAG TGGAAAGACA GCTCTGGCTG CCAAAATCTC AGAGGACTCG 
CAGTTCCCAT TCATTAAGAT CTGCTCTCCA GACAAGATGA TCGGCCACTC AGAGATCTCC 
AAATGCCAGG CAATCAAGAA GATCTTCGAT GATGCCTACA AGTCTCAGCT CAGCTGTGTC 
GTGGTTGATG ACATTGAGCG CCTCTTAGAT TACGTGCCGA TCGGCCCTCG ATTCTCCAAC 
CTGGTGCTTC AAGCCCTGCT GGTCTTACTG AAGAAGACTC CACCTCATGG TCGTAAGCTG 
CTTATCATTG GCACCACCAG CCGTAAAGAC GTCCTGCAGG AGATGGAGAT GCTGGATGCG 
TTCAGCACCA CCATCCACGT GCAGAATATC TCAAGTGGGG ATCATCTGGT TGAAGCTCTA 
GAGCTCCTGG GGAGCTTCAC AGATGCTGAG AGAACCACCA TCGCCCAGCA TGTGAAAGGA 
AAAAGAGTGT GGATTGGCAT TAAGAAGCTG CTCATGCTTA TTGAAATGTC TCTGCAGATG 
GATCAGGAGT ACAGAGTTAG CAAGTTCCTT TCTCTACTCA AAGATGAGGG AGCGCTGGAT 
GAAAACAATC ACATTCGAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Modelling acrylamide acute neurotoxicity in zebrafish larvae.
Scientific reports7(1)13952(2017 Oct)
Prats E,G?mez-Canela C,Ben-Lulu S,Ziv T,Padr?s F,Tornero D,Garcia-Reyero N,Tauler R,Admon A,Rald?a D

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Neuronal Ndrg4 Is Essential for Nodes of Ranvier Organization in Zebrafish.
PLoS genetics12(11)e1006459(2016 Nov)
Fontenas L,De Santis F,Di Donato V,Degerny C,Chambraud B,Del Bene F,Tawk M

Maternal Rest/Nrsf Regulates Zebrafish Behavior through snap25a/b.
The Journal of neuroscience : the official journal of the Society for Neuroscience36(36)9407-19(2016 09)
Moravec CE,Samuel J,Weng W,Wood IC,Sirotkin HI

N-Ethylmaleimide-Sensitive Factor b (nsfb) Is Required for Normal Pigmentation of the Zebrafish Retinal Pigment Epithelium.
Investigative ophthalmology & visual science56(12)7535-44(2015 Nov)
Hanovice NJ,Daly CM,Gross JM