ncln cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ncln
Entrez Gene ID	336844
Full Name	nicalin
Synonyms	ncl1,wu:fb07e09,wu:fk52a06,zgc:77236
General protein information	Preferred Names nicalin Names nicalin-1 nicastrin-like protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005161590.4	XP_005161647.1	nicalin-1 isoform X2
XM_005161591.4	XP_005161648.1	nicalin-1 isoform X3
XM_005161590.3	XP_005161647.1	nicalin-1 isoform X2
XM_005161589.4	XP_005161646.1	nicalin-1 isoform X1
XM_005161589.3	XP_005161646.1	nicalin-1 isoform X1
XM_005161591.3	XP_005161648.1	nicalin-1 isoform X3
NM_207057.1	NP_996940.1	nicalin-1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NCLN	XP_524050.3
Mus musculus (house mouse)	Ncln	NP_598770.1
Gallus gallus (chicken)	NCLN	NP_001026525.1
Arabidopsis thaliana (thale cress)	AT3G44330	NP_190019.1
Canis lupus familiaris (dog)	NCLN	XP_542180.3
Rattus norvegicus (Norway rat)	Ncln	NP_001014104.1
Drosophila melanogaster (fruit fly)	CG4972	NP_609378.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002549	XP_312390.4
Oryza sativa (rice)	Os08g0102100	NP_001060766.1
Homo sapiens (human)	NCLN	NP_064555.2
Bos taurus (cattle)	NCLN	NP_001069423.1
Xenopus tropicalis (tropical clawed frog)	ncln	NP_001025659.1
Danio rerio (zebrafish)	ncl1	NP_996940.1
Caenorhabditis elegans (roundworm)	nra-2	NP_001122514.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Whole exome sequencing coupled with unbiased functional analysis reveals new Hirschsprung disease genes.
Gui H, Schriemer D, Cheng WW, Chauhan RK, Anti?olo G, Berrios C, Bleda M, Brooks AS, Brouwer RW, Burns AJ, Cherny SS, Dopazo J, Eggen BJ, Griseri P, Jalloh B, Le TL, Lui VC, Luz?n-Toro B, Matera I, Ngan ES, Pelet A, Ruiz-Ferrer M, Sham PC, Shepherd IT, So MT, Sribudiani Y, Tang CS, van den Hout MC, van der Linde HC, van Ham TJ, van IJcken WF, Verheij JB, Amiel J, Borrego S, Ceccherini I, Chakravarti A, Lyonnet S, Tam PK, Garcia-Barcel? MM, Hofstra RM
Genome biology18(1)48(2017 Mar)

Evolutionary divergence of the vertebrate TNFAIP8 gene family: Applying the spotted gar orthology bridge to understand ohnolog loss in teleosts.
Sullivan C, Lage CR, Yoder JA, Postlethwait JH, Kim CH
PloS one12(6)e0179517(2017)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Analysis of nicastrin gene phylogeny and expression in zebrafish.
Lim A, Moussavi Nik SH, Ebrahimie E, Lardelli M
Development genes and evolution225(3)171-8(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nicalin/Nodal modulator complex antagonizes Nodal signaling during mesendodermal patterning in zebrafish
Title: Nicalin and its binding partner Nomo are novel Nodal signaling antagonists.

nicalin is associated with protein complexes involved in cell fate decisions during early embryonic development--{review}
Title: The biochemical and genetic odyssey to the function of a nicastrin-like protein.

The following ncln gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ncln cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa37667	NM_207057.1 Latest version!	Danio rerio nicalin (ncln), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa26509	XM_005161589.4 Latest version!	Danio rerio nicalin (ncl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa26510	XM_005161590.4 Latest version!	Danio rerio nicalin (ncl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa26511	XM_005161591.4 Latest version!	Danio rerio nicalin (ncl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa26510	XM_005161590.3	Danio rerio nicalin (ncl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa26509	XM_005161589.3	Danio rerio nicalin (ncl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa26511	XM_005161591.3	Danio rerio nicalin (ncl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ncln ( NM_207057.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_996940.1 Danio rerio nicalin (ncln), mRNA.

Price & Availability↑

CloneID	ODa37667
Clone ID Related Accession (Same CDS sequence)	NM_207057.1
Accession Version	NM_207057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Danio rerio(zebrafish)
Product	nicalin-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC066393.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC066393.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTTCGAGG AGGCTGGTGA GGTGTTGGAG AACATGCTGA AGGTGTCGTT CCCGCTCAGC 
CTGGTGCTGT TTCTGGTGCT CGTGTGTCCG CTGCGCGCGG AAGCGGCGCA TGAGTTCAGT 
GTGTACCGCA TGCAGCAGTA CGACCTGCAG GGGCAAACCT ACGGTTCACG AAATGCCATC 
TTGAACACGG AGGCTCGTAC GGTGGAGGCT GAAGTGCTGA GTCGTCGCTG TGTGATGATG 
CGGCTCGCTG ATTTCTCCTA TGAGAAATAC CAGAAGGCTC TGCGTCAGTC AGCGGGAGCT 
GTGGTCATCA TCCTGCCGCA CAACATGTCC ACCCTACCCC AGGACATAGT GCAGCAGTTC 
ATGGAGCTGG AGCCGGAGCT GTTGGCCACC GAGACCATCG TGCCGGTGTA CTTCGCTCTG 
GAGGACGAGG AGCTGCTGTC TATCTACACA CAGACACAGA TCTCCTCATC CTCACAGGGG 
TCTTCATCCG CCGCTGAAGT GCTGCTTCAT ACGGCTACAG CTAATGGTTT TCAGATGGTG 
ACCAGTGGAG CTCAGAGTAA AGCTGTCAGT GACTGGGCTA TTACTAGTCT AGAGGGCCGC 
CTCACAGGGT CAGGAGGAGA AGATCTGCCC ACCATCGTCC TGGTGGCTCA TTATGACTCT 
TTCGGAGTCG CTCCGTGGCT GTCGTATGGC GCGGACTCAA ACGGCAGTGG CGTCGCTATA 
CTGCTGGAGC TTGCGCGACT GTTTTCCAGA CTGTATTCCT ACAAGAGGAC TCACGCTGGA 
TATAACCTGC TGTTTTTCTT GTCGGGAGGC GGGAAGTTCA ACTATCAAGG CACTAAACGC 
TGGCTAGAGG ACAATCTGGA TCACACAGAT GCCAGTCTGC TCCAGGACAA TGTGGCGTTT 
GTCTTGTGTC TGGACACTCT AGGAAACAGC GATAACCTTC ACCTCCATGT GTCCAAACCA 
CCAAAAGAGG GAAGCCCTCA GTACACCCTG CTCAAAGAGC TAGAAACTGT TGTGGCCCAT 
CAGCACCCCG ACCTGAAGTT CGCGATGGTC CACAAGAAGA TCAACCTGGC GGATGACACT 
CTGGCATGGG AGCACGAGCG CTTCGGGATC CGCCGCCTGC CCGCCTTCAC CCTGTCCCAC 
CTGGAGAGCC ACCGCAGCCC CGCGCGCCAC TCCATCATGG ACATGCGGTC AGTGTCGCCC 
TCTCTGGAGG GGGCAGGAGA GGCCACCACT GGGCCTCATG TGGATCTGGG GAAACTCAGT 
CGTAACACTA AAGTTATTGC AGAAACTCTG GCCAGAGTCA TATATAACCT GACAGAGAAG 
GGCGTCACCG GAGACCTGGA GATCTTCACT GAACAGATGC AGGTGCAGGA GGATCAGCTG 
GCGTCTCTGG TGGACTGGTT GACCGCTCAG CCTCGAGCCG CTCAGCTTCT GGATAAAGAC 
AGCAGCATCA TCAACACGCT GGAGCATCAG CTCAGCCGAT ACCTGAAGGA CGTCAAGAGA 
CACTTGGTGC GAGCAGACAA GAGAGACCCT GAGTTTGTGT TTTATGATCA GCTCAAGCAG 
ACCATGAACG CTTACAGGGT AAAACCAGCA ATCTTTGACC TGCTGTTGGC TGTCTGTATC 
GCATCTTATC TCGGAGTGCT GTATTTGGCC ATTCAGAATT TTGGACTCCT GTATGGTTTT 
CTGCGCAGAG TCACGGCTCC TCGGGTCAAG CAGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996940.1
CDS	108..1826
Translation	MFEEAGEVLENMLKVSFPLSLVLFLVLVCPLRAEAAHEFSVYRMQQYDLQGQTYGSRNAILNTEARTVEAEVLSRRCVMMRLADFSYEKYQKALRQSAGAVVIILPHNMSTLPQDIVQQFMELEPELLATETIVPVYFALEDEELLSIYTQTQISSSSQGSSSAAEVLLHTATANGFQMVTSGAQSKAVSDWAITSLEGRLTGSGGEDLPTIVLVAHYDSFGVAPWLSYGADSNGSGVAILLELARLFSRLYSYKRTHAGYNLLFFLSGGGKFNYQGTKRWLEDNLDHTDASLLQDNVAFVLCLDTLGNSDNLHLHVSKPPKEGSPQYTLLKELETVVAHQHPDLKFAMVHKKINLADDTLAWEHERFGIRRLPAFTLSHLESHRSPARHSIMDMRSVSPSLEGAGEATTGPHVDLGKLSRNTKVIAETLARVIYNLTEKGVTGDLEIFTEQMQVQEDQLASLVDWLTAQPRAAQLLDKDSSIINTLEHQLSRYLKDVKRHLVRADKRDPEFVFYDQLKQTMNAYRVKPAIFDLLLAVCIASYLGVLYLAIQNFGLLYGFLRRVTAPRVKQH

Target ORF information:

RefSeq Version	NM_207057.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nicalin (ncln), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207057.1

ORF Insert Sequence:

ATGTTCGAGG AGGCTGGTGA GGTGTTGGAG AACATGCTGA AGGTGTCGTT CCCGCTCAGC 
CTGGTGCTGT TTCTGGTGCT CGTGTGTCCG CTGCGCGCGG AAGCGGCGCA TGAGTTCAGT 
GTGTACCGCA TGCAGCAGTA CGACCTGCAG GGGCAAACCT ACGGTTCACG AAATGCCATC 
TTGAACACGG AGGCTCGTAC GGTGGAGGCT GAAGTGCTGA GTCGTCGCTG TGTGATGATG 
CGGCTCGCTG ATTTCTCCTA TGAGAAATAC CAGAAGGCTC TGCGTCAGTC AGCGGGAGCT 
GTGGTCATCA TCCTGCCGCA CAACATGTCC ACCCTACCCC AGGACATAGT GCAGCAGTTC 
ATGGAGCTGG AGCCGGAGCT GTTGGCCACC GAGACCATCG TGCCGGTGTA CTTCGCTCTG 
GAGGACGAGG AGCTGCTGTC TATCTACACA CAGACACAGA TCTCCTCATC CTCACAGGGG 
TCTTCATCCG CCGCTGAAGT GCTGCTTCAT ACGGCTACAG CTAATGGTTT TCAGATGGTG 
ACCAGTGGAG CTCAGAGTAA AGCTGTCAGT GACTGGGCTA TTACTAGTCT AGAGGGCCGC 
CTCACAGGGT CAGGAGGAGA AGATCTGCCC ACCATCGTCC TGGTGGCTCA TTATGACTCT 
TTCGGAGTCG CTCCGTGGCT GTCGTATGGC GCGGACTCAA ACGGCAGTGG CGTCGCTATA 
CTGCTGGAGC TTGCGCGACT GTTTTCCAGA CTGTATTCCT ACAAGAGGAC TCACGCTGGA 
TATAACCTGC TGTTTTTCTT GTCGGGAGGC GGGAAGTTCA ACTATCAAGG CACTAAACGC 
TGGCTAGAGG ACAATCTGGA TCACACAGAT GCCAGTCTGC TCCAGGACAA TGTGGCGTTT 
GTCTTGTGTC TGGACACTCT AGGAAACAGC GATAACCTTC ACCTCCATGT GTCCAAACCA 
CCAAAAGAGG GAAGCCCTCA GTACACCCTG CTCAAAGAGC TAGAAACTGT TGTGGCCCAT 
CAGCACCCCG ACCTGAAGTT CGCGATGGTC CACAAGAAGA TCAACCTGGC GGATGACACT 
CTGGCATGGG AGCACGAGCG CTTCGGGATC CGCCGCCTGC CCGCCTTCAC CCTGTCCCAC 
CTGGAGAGCC ACCGCAGCCC CGCGCGCCAC TCCATCATGG ACATGCGGTC AGTGTCGCCC 
TCTCTGGAGG GGGCAGGAGA GGCCACCACT GGGCCTCATG TGGATCTGGG GAAACTCAGT 
CGTAACACTA AAGTTATTGC AGAAACTCTG GCCAGAGTCA TATATAACCT GACAGAGAAG 
GGCGTCACCG GAGACCTGGA GATCTTCACT GAACAGATGC AGGTGCAGGA GGATCAGCTG 
GCGTCTCTGG TGGACTGGTT GACCGCTCAG CCTCGAGCCG CTCAGCTTCT GGATAAAGAC 
AGCAGCATCA TCAACACGCT GGAGCATCAG CTCAGCCGAT ACCTGAAGGA CGTCAAGAGA 
CACTTGGTGC GAGCAGACAA GAGAGACCCT GAGTTTGTGT TTTATGATCA GCTCAAGCAG 
ACCATGAACG CTTACAGGGT AAAACCAGCA ATCTTTGACC TGCTGTTGGC TGTCTGTATC 
GCATCTTATC TCGGAGTGCT GTATTTGGCC ATTCAGAATT TTGGACTCCT GTATGGTTTT 
CTGCGCAGAG TCACGGCTCC TCGGGTCAAG CAGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncln ( XM_005161589.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161646.1 Danio rerio nicalin (ncl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26509
Clone ID Related Accession (Same CDS sequence)	XM_005161589.4 , XM_005161589.3
Accession Version	XM_005161589.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nicalin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161589.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGAGG AGGCTGGTGA GGTGTTGGAG AACATGCTGA AGGTGTCGTT CCCGCTCAGC 
CTGGTGCTGT TTCTGGTGCT CGTGTGTCCG CTGCGCGCGG AAGCGGCGCA TGAGTTCAGT 
GTGTACCGCA TGCAGCAGTA CGACCTGCAG GGGCAAACCT ACGGTTCACG TAATGCCATC 
TTGAACACGG AGGCTCGTAC GGTGGAGGCT GAAGTGCTGA GTCGTCGCTG TGTGATGATG 
CGGCTCGCTG ATTTCTCCTA TGAGAAATAC CAGAAGGCTC TGCGTCAGTC AGCGGGAGCG 
GTGGTCATCA TCCTGCCGCA CAACATGTCC ACCCTACCCC AGGACATAGT GCAGCAGTTC 
ATGGAGCTGG AGCCGGAGCT GTTGGCCACC GAGACCATCG TGCCGGTGTA CTTCGCTCTG 
GAGGACGAGG AGCTGCTGTC TATCTACACA CAGACACAGA TCTCCTCATC CTCACAGGGG 
TCTTCATCCG CCGCTGAAGT GCTGCTTCAT ACGGCTACAG CTAATGGTTT TCAGATGGTG 
ACCAGTGGAG CTCAGAGTAA AGCTGTCAGT GACTGGGCTA TTACTAGTCT AGAGGGCCGC 
CTCACAGGGT CAGGAGGAGA AGATCTGCCC ACCATCGTCC TGGTGGCTCA TTATGACTCT 
TTCGGAGTCG CTCCGTGGCT GTCGTATGGC GCGGACTCAA ACGGCAGTGG CGTCGCTATA 
CTGCTGGAGC TTGCGCGACT GTTTTCCAGA CTGTATTCCT ACAAGAGGAC TCACGCTGGA 
TATAACCTGC TGTTTTTCTT GTCGGGAGGC GGAAAGTTCA ACTATCAAGG CACTAAACGC 
TGGCTAGAGG ACAATCTGGA TCACACAGAT GCCAGTCTGC TCCAGGACAA TGTGGCGTTT 
GTCTTGTGTC TGGACACTCT AGGAAACAGC GATAACCTTC ACCTCCATGT GTCCAAACCA 
CCAAAAGAGG GAAGCCCTCA GTACACCCTG CTCAAAGAGC TAGAAACTGT TGTGGCCCAT 
CAGCACCCCG ACCTGAAGTT CGCGATGGTC CACAAGAAGA TCAACCTGGC GGATGACACT 
CTGGCATGGG AGCACGAGCG CTTCGGGATC CGCCGCCTGC CCGCCTTCAC CCTGTCCCAC 
CTGGAGAGCC ACCGCAGCCC CGCGCGCCAC TCCATCATGG ACATGCGGTC AGTGTCGCCC 
TCTCTGGAGG GGGCAGGAGA GGCCACCACT GGGCCTCATG TGGATCTGGG GAAACTCAGT 
CGTAACACTA AAGTTATTGC AGAAACTCTG GCCAGAGTCA TATATAACCT GACGGAGAAG 
GGCGTCACCG GAGACCTGGA GATCTTCACT GAACAGATGG TGCAGGAGGA TCAGCTGGCG 
TCTCTGGTGG ACTGGTTGAC CGCTCAGCCT CGAGCCGCTC AGCTTCTGGA TAAAGACAGC 
AGCATCATCA ACACGCTGGA GCATCAGCTC AGCCGATACC TGAAGGACGT CAAGAGACAC 
TTGGTGCGAG CAGACAAGAG AGACCCTGAG TTTGTGTTTT ATGATCAGCT CAAGCAGACC 
ATGAATGCTT ACAGGGTAAA ACCAGCAATC TTTGACCTGC TGTTGGCCGT CTGTATCGCA 
TCTTATCTCG GAGTGCTGTA TTTGGCCATT CAGAATTTTG GACTCCTGTA TGGTTTTCTG 
CGCAGAGTCA CGGCTCCTCG GGTCAAGCAG CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161646.1
CDS	111..1826
Translation	MFEEAGEVLENMLKVSFPLSLVLFLVLVCPLRAEAAHEFSVYRMQQYDLQGQTYGSRNAILNTEARTVEAEVLSRRCVMMRLADFSYEKYQKALRQSAGAVVIILPHNMSTLPQDIVQQFMELEPELLATETIVPVYFALEDEELLSIYTQTQISSSSQGSSSAAEVLLHTATANGFQMVTSGAQSKAVSDWAITSLEGRLTGSGGEDLPTIVLVAHYDSFGVAPWLSYGADSNGSGVAILLELARLFSRLYSYKRTHAGYNLLFFLSGGGKFNYQGTKRWLEDNLDHTDASLLQDNVAFVLCLDTLGNSDNLHLHVSKPPKEGSPQYTLLKELETVVAHQHPDLKFAMVHKKINLADDTLAWEHERFGIRRLPAFTLSHLESHRSPARHSIMDMRSVSPSLEGAGEATTGPHVDLGKLSRNTKVIAETLARVIYNLTEKGVTGDLEIFTEQMVQEDQLASLVDWLTAQPRAAQLLDKDSSIINTLEHQLSRYLKDVKRHLVRADKRDPEFVFYDQLKQTMNAYRVKPAIFDLLLAVCIASYLGVLYLAIQNFGLLYGFLRRVTAPRVKQH

Target ORF information:

RefSeq Version	XM_005161589.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nicalin (ncl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161589.4

ORF Insert Sequence:

ATGTTCGAGG AGGCTGGTGA GGTGTTGGAG AACATGCTGA AGGTGTCGTT CCCGCTCAGC 
CTGGTGCTGT TTCTGGTGCT CGTGTGTCCG CTGCGCGCGG AAGCGGCGCA TGAGTTCAGT 
GTGTACCGCA TGCAGCAGTA CGACCTGCAG GGGCAAACCT ACGGTTCACG TAATGCCATC 
TTGAACACGG AGGCTCGTAC GGTGGAGGCT GAAGTGCTGA GTCGTCGCTG TGTGATGATG 
CGGCTCGCTG ATTTCTCCTA TGAGAAATAC CAGAAGGCTC TGCGTCAGTC AGCGGGAGCG 
GTGGTCATCA TCCTGCCGCA CAACATGTCC ACCCTACCCC AGGACATAGT GCAGCAGTTC 
ATGGAGCTGG AGCCGGAGCT GTTGGCCACC GAGACCATCG TGCCGGTGTA CTTCGCTCTG 
GAGGACGAGG AGCTGCTGTC TATCTACACA CAGACACAGA TCTCCTCATC CTCACAGGGG 
TCTTCATCCG CCGCTGAAGT GCTGCTTCAT ACGGCTACAG CTAATGGTTT TCAGATGGTG 
ACCAGTGGAG CTCAGAGTAA AGCTGTCAGT GACTGGGCTA TTACTAGTCT AGAGGGCCGC 
CTCACAGGGT CAGGAGGAGA AGATCTGCCC ACCATCGTCC TGGTGGCTCA TTATGACTCT 
TTCGGAGTCG CTCCGTGGCT GTCGTATGGC GCGGACTCAA ACGGCAGTGG CGTCGCTATA 
CTGCTGGAGC TTGCGCGACT GTTTTCCAGA CTGTATTCCT ACAAGAGGAC TCACGCTGGA 
TATAACCTGC TGTTTTTCTT GTCGGGAGGC GGAAAGTTCA ACTATCAAGG CACTAAACGC 
TGGCTAGAGG ACAATCTGGA TCACACAGAT GCCAGTCTGC TCCAGGACAA TGTGGCGTTT 
GTCTTGTGTC TGGACACTCT AGGAAACAGC GATAACCTTC ACCTCCATGT GTCCAAACCA 
CCAAAAGAGG GAAGCCCTCA GTACACCCTG CTCAAAGAGC TAGAAACTGT TGTGGCCCAT 
CAGCACCCCG ACCTGAAGTT CGCGATGGTC CACAAGAAGA TCAACCTGGC GGATGACACT 
CTGGCATGGG AGCACGAGCG CTTCGGGATC CGCCGCCTGC CCGCCTTCAC CCTGTCCCAC 
CTGGAGAGCC ACCGCAGCCC CGCGCGCCAC TCCATCATGG ACATGCGGTC AGTGTCGCCC 
TCTCTGGAGG GGGCAGGAGA GGCCACCACT GGGCCTCATG TGGATCTGGG GAAACTCAGT 
CGTAACACTA AAGTTATTGC AGAAACTCTG GCCAGAGTCA TATATAACCT GACGGAGAAG 
GGCGTCACCG GAGACCTGGA GATCTTCACT GAACAGATGG TGCAGGAGGA TCAGCTGGCG 
TCTCTGGTGG ACTGGTTGAC CGCTCAGCCT CGAGCCGCTC AGCTTCTGGA TAAAGACAGC 
AGCATCATCA ACACGCTGGA GCATCAGCTC AGCCGATACC TGAAGGACGT CAAGAGACAC 
TTGGTGCGAG CAGACAAGAG AGACCCTGAG TTTGTGTTTT ATGATCAGCT CAAGCAGACC 
ATGAATGCTT ACAGGGTAAA ACCAGCAATC TTTGACCTGC TGTTGGCCGT CTGTATCGCA 
TCTTATCTCG GAGTGCTGTA TTTGGCCATT CAGAATTTTG GACTCCTGTA TGGTTTTCTG 
CGCAGAGTCA CGGCTCCTCG GGTCAAGCAG CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncln ( XM_005161589.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161646.1 Danio rerio nicalin (ncl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26509
Clone ID Related Accession (Same CDS sequence)	XM_005161589.4 , XM_005161589.3
Accession Version	XM_005161589.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nicalin-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161589.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGAGG AGGCTGGTGA GGTGTTGGAG AACATGCTGA AGGTGTCGTT CCCGCTCAGC 
CTGGTGCTGT TTCTGGTGCT CGTGTGTCCG CTGCGCGCGG AAGCGGCGCA TGAGTTCAGT 
GTGTACCGCA TGCAGCAGTA CGACCTGCAG GGGCAAACCT ACGGTTCACG TAATGCCATC 
TTGAACACGG AGGCTCGTAC GGTGGAGGCT GAAGTGCTGA GTCGTCGCTG TGTGATGATG 
CGGCTCGCTG ATTTCTCCTA TGAGAAATAC CAGAAGGCTC TGCGTCAGTC AGCGGGAGCG 
GTGGTCATCA TCCTGCCGCA CAACATGTCC ACCCTACCCC AGGACATAGT GCAGCAGTTC 
ATGGAGCTGG AGCCGGAGCT GTTGGCCACC GAGACCATCG TGCCGGTGTA CTTCGCTCTG 
GAGGACGAGG AGCTGCTGTC TATCTACACA CAGACACAGA TCTCCTCATC CTCACAGGGG 
TCTTCATCCG CCGCTGAAGT GCTGCTTCAT ACGGCTACAG CTAATGGTTT TCAGATGGTG 
ACCAGTGGAG CTCAGAGTAA AGCTGTCAGT GACTGGGCTA TTACTAGTCT AGAGGGCCGC 
CTCACAGGGT CAGGAGGAGA AGATCTGCCC ACCATCGTCC TGGTGGCTCA TTATGACTCT 
TTCGGAGTCG CTCCGTGGCT GTCGTATGGC GCGGACTCAA ACGGCAGTGG CGTCGCTATA 
CTGCTGGAGC TTGCGCGACT GTTTTCCAGA CTGTATTCCT ACAAGAGGAC TCACGCTGGA 
TATAACCTGC TGTTTTTCTT GTCGGGAGGC GGAAAGTTCA ACTATCAAGG CACTAAACGC 
TGGCTAGAGG ACAATCTGGA TCACACAGAT GCCAGTCTGC TCCAGGACAA TGTGGCGTTT 
GTCTTGTGTC TGGACACTCT AGGAAACAGC GATAACCTTC ACCTCCATGT GTCCAAACCA 
CCAAAAGAGG GAAGCCCTCA GTACACCCTG CTCAAAGAGC TAGAAACTGT TGTGGCCCAT 
CAGCACCCCG ACCTGAAGTT CGCGATGGTC CACAAGAAGA TCAACCTGGC GGATGACACT 
CTGGCATGGG AGCACGAGCG CTTCGGGATC CGCCGCCTGC CCGCCTTCAC CCTGTCCCAC 
CTGGAGAGCC ACCGCAGCCC CGCGCGCCAC TCCATCATGG ACATGCGGTC AGTGTCGCCC 
TCTCTGGAGG GGGCAGGAGA GGCCACCACT GGGCCTCATG TGGATCTGGG GAAACTCAGT 
CGTAACACTA AAGTTATTGC AGAAACTCTG GCCAGAGTCA TATATAACCT GACGGAGAAG 
GGCGTCACCG GAGACCTGGA GATCTTCACT GAACAGATGG TGCAGGAGGA TCAGCTGGCG 
TCTCTGGTGG ACTGGTTGAC CGCTCAGCCT CGAGCCGCTC AGCTTCTGGA TAAAGACAGC 
AGCATCATCA ACACGCTGGA GCATCAGCTC AGCCGATACC TGAAGGACGT CAAGAGACAC 
TTGGTGCGAG CAGACAAGAG AGACCCTGAG TTTGTGTTTT ATGATCAGCT CAAGCAGACC 
ATGAATGCTT ACAGGGTAAA ACCAGCAATC TTTGACCTGC TGTTGGCCGT CTGTATCGCA 
TCTTATCTCG GAGTGCTGTA TTTGGCCATT CAGAATTTTG GACTCCTGTA TGGTTTTCTG 
CGCAGAGTCA CGGCTCCTCG GGTCAAGCAG CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161646.1
CDS	143..1858
Translation	MFEEAGEVLENMLKVSFPLSLVLFLVLVCPLRAEAAHEFSVYRMQQYDLQGQTYGSRNAILNTEARTVEAEVLSRRCVMMRLADFSYEKYQKALRQSAGAVVIILPHNMSTLPQDIVQQFMELEPELLATETIVPVYFALEDEELLSIYTQTQISSSSQGSSSAAEVLLHTATANGFQMVTSGAQSKAVSDWAITSLEGRLTGSGGEDLPTIVLVAHYDSFGVAPWLSYGADSNGSGVAILLELARLFSRLYSYKRTHAGYNLLFFLSGGGKFNYQGTKRWLEDNLDHTDASLLQDNVAFVLCLDTLGNSDNLHLHVSKPPKEGSPQYTLLKELETVVAHQHPDLKFAMVHKKINLADDTLAWEHERFGIRRLPAFTLSHLESHRSPARHSIMDMRSVSPSLEGAGEATTGPHVDLGKLSRNTKVIAETLARVIYNLTEKGVTGDLEIFTEQMVQEDQLASLVDWLTAQPRAAQLLDKDSSIINTLEHQLSRYLKDVKRHLVRADKRDPEFVFYDQLKQTMNAYRVKPAIFDLLLAVCIASYLGVLYLAIQNFGLLYGFLRRVTAPRVKQH

Target ORF information:

RefSeq Version	XM_005161589.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nicalin (ncl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161589.3

ORF Insert Sequence:

ATGTTCGAGG AGGCTGGTGA GGTGTTGGAG AACATGCTGA AGGTGTCGTT CCCGCTCAGC 
CTGGTGCTGT TTCTGGTGCT CGTGTGTCCG CTGCGCGCGG AAGCGGCGCA TGAGTTCAGT 
GTGTACCGCA TGCAGCAGTA CGACCTGCAG GGGCAAACCT ACGGTTCACG TAATGCCATC 
TTGAACACGG AGGCTCGTAC GGTGGAGGCT GAAGTGCTGA GTCGTCGCTG TGTGATGATG 
CGGCTCGCTG ATTTCTCCTA TGAGAAATAC CAGAAGGCTC TGCGTCAGTC AGCGGGAGCG 
GTGGTCATCA TCCTGCCGCA CAACATGTCC ACCCTACCCC AGGACATAGT GCAGCAGTTC 
ATGGAGCTGG AGCCGGAGCT GTTGGCCACC GAGACCATCG TGCCGGTGTA CTTCGCTCTG 
GAGGACGAGG AGCTGCTGTC TATCTACACA CAGACACAGA TCTCCTCATC CTCACAGGGG 
TCTTCATCCG CCGCTGAAGT GCTGCTTCAT ACGGCTACAG CTAATGGTTT TCAGATGGTG 
ACCAGTGGAG CTCAGAGTAA AGCTGTCAGT GACTGGGCTA TTACTAGTCT AGAGGGCCGC 
CTCACAGGGT CAGGAGGAGA AGATCTGCCC ACCATCGTCC TGGTGGCTCA TTATGACTCT 
TTCGGAGTCG CTCCGTGGCT GTCGTATGGC GCGGACTCAA ACGGCAGTGG CGTCGCTATA 
CTGCTGGAGC TTGCGCGACT GTTTTCCAGA CTGTATTCCT ACAAGAGGAC TCACGCTGGA 
TATAACCTGC TGTTTTTCTT GTCGGGAGGC GGAAAGTTCA ACTATCAAGG CACTAAACGC 
TGGCTAGAGG ACAATCTGGA TCACACAGAT GCCAGTCTGC TCCAGGACAA TGTGGCGTTT 
GTCTTGTGTC TGGACACTCT AGGAAACAGC GATAACCTTC ACCTCCATGT GTCCAAACCA 
CCAAAAGAGG GAAGCCCTCA GTACACCCTG CTCAAAGAGC TAGAAACTGT TGTGGCCCAT 
CAGCACCCCG ACCTGAAGTT CGCGATGGTC CACAAGAAGA TCAACCTGGC GGATGACACT 
CTGGCATGGG AGCACGAGCG CTTCGGGATC CGCCGCCTGC CCGCCTTCAC CCTGTCCCAC 
CTGGAGAGCC ACCGCAGCCC CGCGCGCCAC TCCATCATGG ACATGCGGTC AGTGTCGCCC 
TCTCTGGAGG GGGCAGGAGA GGCCACCACT GGGCCTCATG TGGATCTGGG GAAACTCAGT 
CGTAACACTA AAGTTATTGC AGAAACTCTG GCCAGAGTCA TATATAACCT GACGGAGAAG 
GGCGTCACCG GAGACCTGGA GATCTTCACT GAACAGATGG TGCAGGAGGA TCAGCTGGCG 
TCTCTGGTGG ACTGGTTGAC CGCTCAGCCT CGAGCCGCTC AGCTTCTGGA TAAAGACAGC 
AGCATCATCA ACACGCTGGA GCATCAGCTC AGCCGATACC TGAAGGACGT CAAGAGACAC 
TTGGTGCGAG CAGACAAGAG AGACCCTGAG TTTGTGTTTT ATGATCAGCT CAAGCAGACC 
ATGAATGCTT ACAGGGTAAA ACCAGCAATC TTTGACCTGC TGTTGGCCGT CTGTATCGCA 
TCTTATCTCG GAGTGCTGTA TTTGGCCATT CAGAATTTTG GACTCCTGTA TGGTTTTCTG 
CGCAGAGTCA CGGCTCCTCG GGTCAAGCAG CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncln ( XM_005161590.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161647.1 Danio rerio nicalin (ncl1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa26510
Clone ID Related Accession (Same CDS sequence)	XM_005161590.4 , XM_005161590.3
Accession Version	XM_005161590.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nicalin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161590.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGAGG AGGCTGGTGA GGTGTTGGAG AACATGCTGA AGGTGTCGTT CCCGCTCAGC 
CTGGTGCTGT TTCTGGTGCT CGTGTGTCCG CTGCGCGCGG AAGCGGCGCA TGAGTTCAGT 
GTGTACCGCA TGCAGCAGTA CGACCTGCAG GGGCAAACCT ACGGTTCACG TAATGCCATC 
TTGAACACGG AGGCTCGTAC GGTGGAGGCT GAAGTGCTGA GTCGTCGCTG TGTGATGATG 
CGGCTCGCTG ATTTCTCCTA TGAGAAATAC CAGAAGGCTC TGCGTCAGTC AGCGGGAGCG 
GTGGTCATCA TCCTGCCGCA CAACATGTCC ACCCTACCCC AGGACATAGT GCAGCAGTTC 
ATGGAGCTGG AGCCGGAGCT GTTGGCCACC GAGACCATCG TGCCGGTGTA CTTCGCTCTG 
GAGGACGAGG AGCTGCTGTC TATCTACACA CAGACACAGA TCTCCTCATC CTCACAGGGG 
TCTTCATCCG CCGCTGAAGT GCTGCTTCAT ACGGCTACAG CTAATGGTTT TCAGATGGTG 
ACCAGTGGAG CTCAGAGTAA AGCTGTCAGT GACTGGGCTA TTACTAGTCT AGAGGGCCGC 
CTCACAGGGT CAGGAGGAGA AGATCTGCCC ACCATCGTCC TGGTGGCTCA TTATGACTCT 
TTCGGAGTCG CTCCGTGGCT GTCGTATGGC GCGGACTCAA ACGGCAGTGG CGTCGCTATA 
CTGCTGGAGC TTGCGCGACT GTTTTCCAGA CTGTATTCCT ACAAGAGGAC TCACGCTGGA 
TATAACCTGC TGTTTTTCTT GTCGGGAGGC GGAAAGTTCA ACTATCAAGG CACTAAACGC 
TGGCTAGAGG ACAATCTGGA TCACACAGAT GCCAGTCTGC TCCAGGACAA TGTGGCGTTT 
GTCTTGTGTC TGGACACTCT AGGAAACAGC GATAACCTTC ACCTCCATGT GTCCAAACCA 
CCAAAAGAGG GAAGCCCTCA GTACACCCTG CTCAAAGAGC TAGAAACTGT TGTGGCCCAT 
CAGCACCCCG ACCTGAAGTT CGCGATGGTC CACAAGAAGA TCAACCTGGC GGATGACACT 
CTGGCATGGG AGCACGAGCG CTTCGGGATC CGCCGCCTGC CCGCCTTCAC CCTGTCCCAC 
CTGGAGAGCC ACCGCAGCCC CGCGCGCCAC TCCATCATGG ACATGCGGCC TCATGTGGAT 
CTGGGGAAAC TCAGTCGTAA CACTAAAGTT ATTGCAGAAA CTCTGGCCAG AGTCATATAT 
AACCTGACGG AGAAGGGCGT CACCGGAGAC CTGGAGATCT TCACTGAACA GATGCAGGTG 
CAGGAGGATC AGCTGGCGTC TCTGGTGGAC TGGTTGACCG CTCAGCCTCG AGCCGCTCAG 
CTTCTGGATA AAGACAGCAG CATCATCAAC ACGCTGGAGC ATCAGCTCAG CCGATACCTG 
AAGGACGTCA AGAGACACTT GGTGCGAGCA GACAAGAGAG ACCCTGAGTT TGTGTTTTAT 
GATCAGCTCA AGCAGACCAT GAATGCTTAC AGGGTAAAAC CAGCAATCTT TGACCTGCTG 
TTGGCCGTCT GTATCGCATC TTATCTCGGA GTGCTGTATT TGGCCATTCA GAATTTTGGA 
CTCCTGTATG GTTTTCTGCG CAGAGTCACG GCTCCTCGGG TCAAGCAGCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161647.1
CDS	111..1784
Translation	MFEEAGEVLENMLKVSFPLSLVLFLVLVCPLRAEAAHEFSVYRMQQYDLQGQTYGSRNAILNTEARTVEAEVLSRRCVMMRLADFSYEKYQKALRQSAGAVVIILPHNMSTLPQDIVQQFMELEPELLATETIVPVYFALEDEELLSIYTQTQISSSSQGSSSAAEVLLHTATANGFQMVTSGAQSKAVSDWAITSLEGRLTGSGGEDLPTIVLVAHYDSFGVAPWLSYGADSNGSGVAILLELARLFSRLYSYKRTHAGYNLLFFLSGGGKFNYQGTKRWLEDNLDHTDASLLQDNVAFVLCLDTLGNSDNLHLHVSKPPKEGSPQYTLLKELETVVAHQHPDLKFAMVHKKINLADDTLAWEHERFGIRRLPAFTLSHLESHRSPARHSIMDMRPHVDLGKLSRNTKVIAETLARVIYNLTEKGVTGDLEIFTEQMQVQEDQLASLVDWLTAQPRAAQLLDKDSSIINTLEHQLSRYLKDVKRHLVRADKRDPEFVFYDQLKQTMNAYRVKPAIFDLLLAVCIASYLGVLYLAIQNFGLLYGFLRRVTAPRVKQH

Target ORF information:

RefSeq Version	XM_005161590.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nicalin (ncl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161590.4

ORF Insert Sequence:

ATGTTCGAGG AGGCTGGTGA GGTGTTGGAG AACATGCTGA AGGTGTCGTT CCCGCTCAGC 
CTGGTGCTGT TTCTGGTGCT CGTGTGTCCG CTGCGCGCGG AAGCGGCGCA TGAGTTCAGT 
GTGTACCGCA TGCAGCAGTA CGACCTGCAG GGGCAAACCT ACGGTTCACG TAATGCCATC 
TTGAACACGG AGGCTCGTAC GGTGGAGGCT GAAGTGCTGA GTCGTCGCTG TGTGATGATG 
CGGCTCGCTG ATTTCTCCTA TGAGAAATAC CAGAAGGCTC TGCGTCAGTC AGCGGGAGCG 
GTGGTCATCA TCCTGCCGCA CAACATGTCC ACCCTACCCC AGGACATAGT GCAGCAGTTC 
ATGGAGCTGG AGCCGGAGCT GTTGGCCACC GAGACCATCG TGCCGGTGTA CTTCGCTCTG 
GAGGACGAGG AGCTGCTGTC TATCTACACA CAGACACAGA TCTCCTCATC CTCACAGGGG 
TCTTCATCCG CCGCTGAAGT GCTGCTTCAT ACGGCTACAG CTAATGGTTT TCAGATGGTG 
ACCAGTGGAG CTCAGAGTAA AGCTGTCAGT GACTGGGCTA TTACTAGTCT AGAGGGCCGC 
CTCACAGGGT CAGGAGGAGA AGATCTGCCC ACCATCGTCC TGGTGGCTCA TTATGACTCT 
TTCGGAGTCG CTCCGTGGCT GTCGTATGGC GCGGACTCAA ACGGCAGTGG CGTCGCTATA 
CTGCTGGAGC TTGCGCGACT GTTTTCCAGA CTGTATTCCT ACAAGAGGAC TCACGCTGGA 
TATAACCTGC TGTTTTTCTT GTCGGGAGGC GGAAAGTTCA ACTATCAAGG CACTAAACGC 
TGGCTAGAGG ACAATCTGGA TCACACAGAT GCCAGTCTGC TCCAGGACAA TGTGGCGTTT 
GTCTTGTGTC TGGACACTCT AGGAAACAGC GATAACCTTC ACCTCCATGT GTCCAAACCA 
CCAAAAGAGG GAAGCCCTCA GTACACCCTG CTCAAAGAGC TAGAAACTGT TGTGGCCCAT 
CAGCACCCCG ACCTGAAGTT CGCGATGGTC CACAAGAAGA TCAACCTGGC GGATGACACT 
CTGGCATGGG AGCACGAGCG CTTCGGGATC CGCCGCCTGC CCGCCTTCAC CCTGTCCCAC 
CTGGAGAGCC ACCGCAGCCC CGCGCGCCAC TCCATCATGG ACATGCGGCC TCATGTGGAT 
CTGGGGAAAC TCAGTCGTAA CACTAAAGTT ATTGCAGAAA CTCTGGCCAG AGTCATATAT 
AACCTGACGG AGAAGGGCGT CACCGGAGAC CTGGAGATCT TCACTGAACA GATGCAGGTG 
CAGGAGGATC AGCTGGCGTC TCTGGTGGAC TGGTTGACCG CTCAGCCTCG AGCCGCTCAG 
CTTCTGGATA AAGACAGCAG CATCATCAAC ACGCTGGAGC ATCAGCTCAG CCGATACCTG 
AAGGACGTCA AGAGACACTT GGTGCGAGCA GACAAGAGAG ACCCTGAGTT TGTGTTTTAT 
GATCAGCTCA AGCAGACCAT GAATGCTTAC AGGGTAAAAC CAGCAATCTT TGACCTGCTG 
TTGGCCGTCT GTATCGCATC TTATCTCGGA GTGCTGTATT TGGCCATTCA GAATTTTGGA 
CTCCTGTATG GTTTTCTGCG CAGAGTCACG GCTCCTCGGG TCAAGCAGCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncln ( XM_005161590.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161647.1 Danio rerio nicalin (ncl1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa26510
Clone ID Related Accession (Same CDS sequence)	XM_005161590.4 , XM_005161590.3
Accession Version	XM_005161590.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nicalin-1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161590.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGAGG AGGCTGGTGA GGTGTTGGAG AACATGCTGA AGGTGTCGTT CCCGCTCAGC 
CTGGTGCTGT TTCTGGTGCT CGTGTGTCCG CTGCGCGCGG AAGCGGCGCA TGAGTTCAGT 
GTGTACCGCA TGCAGCAGTA CGACCTGCAG GGGCAAACCT ACGGTTCACG TAATGCCATC 
TTGAACACGG AGGCTCGTAC GGTGGAGGCT GAAGTGCTGA GTCGTCGCTG TGTGATGATG 
CGGCTCGCTG ATTTCTCCTA TGAGAAATAC CAGAAGGCTC TGCGTCAGTC AGCGGGAGCG 
GTGGTCATCA TCCTGCCGCA CAACATGTCC ACCCTACCCC AGGACATAGT GCAGCAGTTC 
ATGGAGCTGG AGCCGGAGCT GTTGGCCACC GAGACCATCG TGCCGGTGTA CTTCGCTCTG 
GAGGACGAGG AGCTGCTGTC TATCTACACA CAGACACAGA TCTCCTCATC CTCACAGGGG 
TCTTCATCCG CCGCTGAAGT GCTGCTTCAT ACGGCTACAG CTAATGGTTT TCAGATGGTG 
ACCAGTGGAG CTCAGAGTAA AGCTGTCAGT GACTGGGCTA TTACTAGTCT AGAGGGCCGC 
CTCACAGGGT CAGGAGGAGA AGATCTGCCC ACCATCGTCC TGGTGGCTCA TTATGACTCT 
TTCGGAGTCG CTCCGTGGCT GTCGTATGGC GCGGACTCAA ACGGCAGTGG CGTCGCTATA 
CTGCTGGAGC TTGCGCGACT GTTTTCCAGA CTGTATTCCT ACAAGAGGAC TCACGCTGGA 
TATAACCTGC TGTTTTTCTT GTCGGGAGGC GGAAAGTTCA ACTATCAAGG CACTAAACGC 
TGGCTAGAGG ACAATCTGGA TCACACAGAT GCCAGTCTGC TCCAGGACAA TGTGGCGTTT 
GTCTTGTGTC TGGACACTCT AGGAAACAGC GATAACCTTC ACCTCCATGT GTCCAAACCA 
CCAAAAGAGG GAAGCCCTCA GTACACCCTG CTCAAAGAGC TAGAAACTGT TGTGGCCCAT 
CAGCACCCCG ACCTGAAGTT CGCGATGGTC CACAAGAAGA TCAACCTGGC GGATGACACT 
CTGGCATGGG AGCACGAGCG CTTCGGGATC CGCCGCCTGC CCGCCTTCAC CCTGTCCCAC 
CTGGAGAGCC ACCGCAGCCC CGCGCGCCAC TCCATCATGG ACATGCGGCC TCATGTGGAT 
CTGGGGAAAC TCAGTCGTAA CACTAAAGTT ATTGCAGAAA CTCTGGCCAG AGTCATATAT 
AACCTGACGG AGAAGGGCGT CACCGGAGAC CTGGAGATCT TCACTGAACA GATGCAGGTG 
CAGGAGGATC AGCTGGCGTC TCTGGTGGAC TGGTTGACCG CTCAGCCTCG AGCCGCTCAG 
CTTCTGGATA AAGACAGCAG CATCATCAAC ACGCTGGAGC ATCAGCTCAG CCGATACCTG 
AAGGACGTCA AGAGACACTT GGTGCGAGCA GACAAGAGAG ACCCTGAGTT TGTGTTTTAT 
GATCAGCTCA AGCAGACCAT GAATGCTTAC AGGGTAAAAC CAGCAATCTT TGACCTGCTG 
TTGGCCGTCT GTATCGCATC TTATCTCGGA GTGCTGTATT TGGCCATTCA GAATTTTGGA 
CTCCTGTATG GTTTTCTGCG CAGAGTCACG GCTCCTCGGG TCAAGCAGCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161647.1
CDS	143..1816
Translation	MFEEAGEVLENMLKVSFPLSLVLFLVLVCPLRAEAAHEFSVYRMQQYDLQGQTYGSRNAILNTEARTVEAEVLSRRCVMMRLADFSYEKYQKALRQSAGAVVIILPHNMSTLPQDIVQQFMELEPELLATETIVPVYFALEDEELLSIYTQTQISSSSQGSSSAAEVLLHTATANGFQMVTSGAQSKAVSDWAITSLEGRLTGSGGEDLPTIVLVAHYDSFGVAPWLSYGADSNGSGVAILLELARLFSRLYSYKRTHAGYNLLFFLSGGGKFNYQGTKRWLEDNLDHTDASLLQDNVAFVLCLDTLGNSDNLHLHVSKPPKEGSPQYTLLKELETVVAHQHPDLKFAMVHKKINLADDTLAWEHERFGIRRLPAFTLSHLESHRSPARHSIMDMRPHVDLGKLSRNTKVIAETLARVIYNLTEKGVTGDLEIFTEQMQVQEDQLASLVDWLTAQPRAAQLLDKDSSIINTLEHQLSRYLKDVKRHLVRADKRDPEFVFYDQLKQTMNAYRVKPAIFDLLLAVCIASYLGVLYLAIQNFGLLYGFLRRVTAPRVKQH

Target ORF information:

RefSeq Version	XM_005161590.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nicalin (ncl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161590.3

ORF Insert Sequence:

ATGTTCGAGG AGGCTGGTGA GGTGTTGGAG AACATGCTGA AGGTGTCGTT CCCGCTCAGC 
CTGGTGCTGT TTCTGGTGCT CGTGTGTCCG CTGCGCGCGG AAGCGGCGCA TGAGTTCAGT 
GTGTACCGCA TGCAGCAGTA CGACCTGCAG GGGCAAACCT ACGGTTCACG TAATGCCATC 
TTGAACACGG AGGCTCGTAC GGTGGAGGCT GAAGTGCTGA GTCGTCGCTG TGTGATGATG 
CGGCTCGCTG ATTTCTCCTA TGAGAAATAC CAGAAGGCTC TGCGTCAGTC AGCGGGAGCG 
GTGGTCATCA TCCTGCCGCA CAACATGTCC ACCCTACCCC AGGACATAGT GCAGCAGTTC 
ATGGAGCTGG AGCCGGAGCT GTTGGCCACC GAGACCATCG TGCCGGTGTA CTTCGCTCTG 
GAGGACGAGG AGCTGCTGTC TATCTACACA CAGACACAGA TCTCCTCATC CTCACAGGGG 
TCTTCATCCG CCGCTGAAGT GCTGCTTCAT ACGGCTACAG CTAATGGTTT TCAGATGGTG 
ACCAGTGGAG CTCAGAGTAA AGCTGTCAGT GACTGGGCTA TTACTAGTCT AGAGGGCCGC 
CTCACAGGGT CAGGAGGAGA AGATCTGCCC ACCATCGTCC TGGTGGCTCA TTATGACTCT 
TTCGGAGTCG CTCCGTGGCT GTCGTATGGC GCGGACTCAA ACGGCAGTGG CGTCGCTATA 
CTGCTGGAGC TTGCGCGACT GTTTTCCAGA CTGTATTCCT ACAAGAGGAC TCACGCTGGA 
TATAACCTGC TGTTTTTCTT GTCGGGAGGC GGAAAGTTCA ACTATCAAGG CACTAAACGC 
TGGCTAGAGG ACAATCTGGA TCACACAGAT GCCAGTCTGC TCCAGGACAA TGTGGCGTTT 
GTCTTGTGTC TGGACACTCT AGGAAACAGC GATAACCTTC ACCTCCATGT GTCCAAACCA 
CCAAAAGAGG GAAGCCCTCA GTACACCCTG CTCAAAGAGC TAGAAACTGT TGTGGCCCAT 
CAGCACCCCG ACCTGAAGTT CGCGATGGTC CACAAGAAGA TCAACCTGGC GGATGACACT 
CTGGCATGGG AGCACGAGCG CTTCGGGATC CGCCGCCTGC CCGCCTTCAC CCTGTCCCAC 
CTGGAGAGCC ACCGCAGCCC CGCGCGCCAC TCCATCATGG ACATGCGGCC TCATGTGGAT 
CTGGGGAAAC TCAGTCGTAA CACTAAAGTT ATTGCAGAAA CTCTGGCCAG AGTCATATAT 
AACCTGACGG AGAAGGGCGT CACCGGAGAC CTGGAGATCT TCACTGAACA GATGCAGGTG 
CAGGAGGATC AGCTGGCGTC TCTGGTGGAC TGGTTGACCG CTCAGCCTCG AGCCGCTCAG 
CTTCTGGATA AAGACAGCAG CATCATCAAC ACGCTGGAGC ATCAGCTCAG CCGATACCTG 
AAGGACGTCA AGAGACACTT GGTGCGAGCA GACAAGAGAG ACCCTGAGTT TGTGTTTTAT 
GATCAGCTCA AGCAGACCAT GAATGCTTAC AGGGTAAAAC CAGCAATCTT TGACCTGCTG 
TTGGCCGTCT GTATCGCATC TTATCTCGGA GTGCTGTATT TGGCCATTCA GAATTTTGGA 
CTCCTGTATG GTTTTCTGCG CAGAGTCACG GCTCCTCGGG TCAAGCAGCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncln ( XM_005161591.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161648.1 Danio rerio nicalin (ncl1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa26511
Clone ID Related Accession (Same CDS sequence)	XM_005161591.4 , XM_005161591.3
Accession Version	XM_005161591.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nicalin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161591.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGAGG AGGCTGGTGA GGTGTTGGAG AACATGCTGA AGGTGTCGTT CCCGCTCAGC 
CTGGTGCTGT TTCTGGTGCT CGTGTGTCCG CTGCGCGCGG AAGCGGCGCA TGAGTTCAGT 
GTGTACCGCA TGCAGCAGTA CGACCTGCAG GGGCAAACCT ACGGTTCACG TAATGCCATC 
TTGAACACGG AGGCTCGTAC GGTGGAGGCT GAAGTGCTGA GTCGTCGCTG TGTGATGATG 
CGGCTCGCTG ATTTCTCCTA TGAGAAATAC CAGAAGGCTC TGCGTCAGTC AGCGGGAGCG 
GTGGTCATCA TCCTGCCGCA CAACATGTCC ACCCTACCCC AGGACATAGT GCAGCAGTTC 
ATGGAGCTGG AGCCGGAGCT GTTGGCCACC GAGACCATCG TGCCGGTGTA CTTCGCTCTG 
GAGGACGAGG AGCTGCTGTC TATCTACACA CAGACACAGA TCTCCTCATC CTCACAGGGG 
TCTTCATCCG CCGCTGAAGT GCTGCTTCAT ACGGCTACAG CTAATGGTTT TCAGATGGTG 
ACCAGTGGAG CTCAGAGTAA AGCTGTCAGT GACTGGGCTA TTACTAGTCT AGAGGGCCGC 
CTCACAGGGT CAGGAGGAGA AGATCTGCCC ACCATCGTCC TGGTGGCTCA TTATGACTCT 
TTCGGAGTCG CTCCGTGGCT GTCGTATGGC GCGGACTCAA ACGGCAGTGG CGTCGCTATA 
CTGCTGGAGC TTGCGCGACT GTTTTCCAGA CTGTATTCCT ACAAGAGGAC TCACGCTGGA 
TATAACCTGC TGTTTTTCTT GTCGGGAGGC GGAAAGTTCA ACTATCAAGG CACTAAACGC 
TGGCTAGAGG ACAATCTGGA TCACACAGAT GCCAGTCTGC TCCAGGACAA TGTGGCGTTT 
GTCTTGTGTC TGGACACTCT AGGAAACAGC GATAACCTTC ACCTCCATGT GTCCAAACCA 
CCAAAAGAGG GAAGCCCTCA GTACACCCTG CTCAAAGAGC TAGAAACTGT TGTGGCCCAT 
CAGCACCCCG ACCTGAAGTT CGCGATGGTC CACAAGAAGA TCAACCTGGC GGATGACACT 
CTGGCATGGG AGCACGAGCG CTTCGGGATC CGCCGCCTGC CCGCCTTCAC CCTGTCCCAC 
CTGGAGAGCC ACCGCAGCCC CGCGCGCCAC TCCATCATGG ACATGCGGCC TCATGTGGAT 
CTGGGGAAAC TCAGTCGTAA CACTAAAGTT ATTGCAGAAA CTCTGGCCAG AGTCATATAT 
AACCTGACGG AGAAGGGCGT CACCGGAGAC CTGGAGATCT TCACTGAACA GATGGTGCAG 
GAGGATCAGC TGGCGTCTCT GGTGGACTGG TTGACCGCTC AGCCTCGAGC CGCTCAGCTT 
CTGGATAAAG ACAGCAGCAT CATCAACACG CTGGAGCATC AGCTCAGCCG ATACCTGAAG 
GACGTCAAGA GACACTTGGT GCGAGCAGAC AAGAGAGACC CTGAGTTTGT GTTTTATGAT 
CAGCTCAAGC AGACCATGAA TGCTTACAGG GTAAAACCAG CAATCTTTGA CCTGCTGTTG 
GCCGTCTGTA TCGCATCTTA TCTCGGAGTG CTGTATTTGG CCATTCAGAA TTTTGGACTC 
CTGTATGGTT TTCTGCGCAG AGTCACGGCT CCTCGGGTCA AGCAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161648.1
CDS	111..1781
Translation	MFEEAGEVLENMLKVSFPLSLVLFLVLVCPLRAEAAHEFSVYRMQQYDLQGQTYGSRNAILNTEARTVEAEVLSRRCVMMRLADFSYEKYQKALRQSAGAVVIILPHNMSTLPQDIVQQFMELEPELLATETIVPVYFALEDEELLSIYTQTQISSSSQGSSSAAEVLLHTATANGFQMVTSGAQSKAVSDWAITSLEGRLTGSGGEDLPTIVLVAHYDSFGVAPWLSYGADSNGSGVAILLELARLFSRLYSYKRTHAGYNLLFFLSGGGKFNYQGTKRWLEDNLDHTDASLLQDNVAFVLCLDTLGNSDNLHLHVSKPPKEGSPQYTLLKELETVVAHQHPDLKFAMVHKKINLADDTLAWEHERFGIRRLPAFTLSHLESHRSPARHSIMDMRPHVDLGKLSRNTKVIAETLARVIYNLTEKGVTGDLEIFTEQMVQEDQLASLVDWLTAQPRAAQLLDKDSSIINTLEHQLSRYLKDVKRHLVRADKRDPEFVFYDQLKQTMNAYRVKPAIFDLLLAVCIASYLGVLYLAIQNFGLLYGFLRRVTAPRVKQH

Target ORF information:

RefSeq Version	XM_005161591.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nicalin (ncl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161591.4

ORF Insert Sequence:

ATGTTCGAGG AGGCTGGTGA GGTGTTGGAG AACATGCTGA AGGTGTCGTT CCCGCTCAGC 
CTGGTGCTGT TTCTGGTGCT CGTGTGTCCG CTGCGCGCGG AAGCGGCGCA TGAGTTCAGT 
GTGTACCGCA TGCAGCAGTA CGACCTGCAG GGGCAAACCT ACGGTTCACG TAATGCCATC 
TTGAACACGG AGGCTCGTAC GGTGGAGGCT GAAGTGCTGA GTCGTCGCTG TGTGATGATG 
CGGCTCGCTG ATTTCTCCTA TGAGAAATAC CAGAAGGCTC TGCGTCAGTC AGCGGGAGCG 
GTGGTCATCA TCCTGCCGCA CAACATGTCC ACCCTACCCC AGGACATAGT GCAGCAGTTC 
ATGGAGCTGG AGCCGGAGCT GTTGGCCACC GAGACCATCG TGCCGGTGTA CTTCGCTCTG 
GAGGACGAGG AGCTGCTGTC TATCTACACA CAGACACAGA TCTCCTCATC CTCACAGGGG 
TCTTCATCCG CCGCTGAAGT GCTGCTTCAT ACGGCTACAG CTAATGGTTT TCAGATGGTG 
ACCAGTGGAG CTCAGAGTAA AGCTGTCAGT GACTGGGCTA TTACTAGTCT AGAGGGCCGC 
CTCACAGGGT CAGGAGGAGA AGATCTGCCC ACCATCGTCC TGGTGGCTCA TTATGACTCT 
TTCGGAGTCG CTCCGTGGCT GTCGTATGGC GCGGACTCAA ACGGCAGTGG CGTCGCTATA 
CTGCTGGAGC TTGCGCGACT GTTTTCCAGA CTGTATTCCT ACAAGAGGAC TCACGCTGGA 
TATAACCTGC TGTTTTTCTT GTCGGGAGGC GGAAAGTTCA ACTATCAAGG CACTAAACGC 
TGGCTAGAGG ACAATCTGGA TCACACAGAT GCCAGTCTGC TCCAGGACAA TGTGGCGTTT 
GTCTTGTGTC TGGACACTCT AGGAAACAGC GATAACCTTC ACCTCCATGT GTCCAAACCA 
CCAAAAGAGG GAAGCCCTCA GTACACCCTG CTCAAAGAGC TAGAAACTGT TGTGGCCCAT 
CAGCACCCCG ACCTGAAGTT CGCGATGGTC CACAAGAAGA TCAACCTGGC GGATGACACT 
CTGGCATGGG AGCACGAGCG CTTCGGGATC CGCCGCCTGC CCGCCTTCAC CCTGTCCCAC 
CTGGAGAGCC ACCGCAGCCC CGCGCGCCAC TCCATCATGG ACATGCGGCC TCATGTGGAT 
CTGGGGAAAC TCAGTCGTAA CACTAAAGTT ATTGCAGAAA CTCTGGCCAG AGTCATATAT 
AACCTGACGG AGAAGGGCGT CACCGGAGAC CTGGAGATCT TCACTGAACA GATGGTGCAG 
GAGGATCAGC TGGCGTCTCT GGTGGACTGG TTGACCGCTC AGCCTCGAGC CGCTCAGCTT 
CTGGATAAAG ACAGCAGCAT CATCAACACG CTGGAGCATC AGCTCAGCCG ATACCTGAAG 
GACGTCAAGA GACACTTGGT GCGAGCAGAC AAGAGAGACC CTGAGTTTGT GTTTTATGAT 
CAGCTCAAGC AGACCATGAA TGCTTACAGG GTAAAACCAG CAATCTTTGA CCTGCTGTTG 
GCCGTCTGTA TCGCATCTTA TCTCGGAGTG CTGTATTTGG CCATTCAGAA TTTTGGACTC 
CTGTATGGTT TTCTGCGCAG AGTCACGGCT CCTCGGGTCA AGCAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncln ( XM_005161591.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161648.1 Danio rerio nicalin (ncl1), transcript variant X3, mRNA.

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CloneID	ODa26511
Clone ID Related Accession (Same CDS sequence)	XM_005161591.4 , XM_005161591.3
Accession Version	XM_005161591.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nicalin-1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161591.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGAGG AGGCTGGTGA GGTGTTGGAG AACATGCTGA AGGTGTCGTT CCCGCTCAGC 
CTGGTGCTGT TTCTGGTGCT CGTGTGTCCG CTGCGCGCGG AAGCGGCGCA TGAGTTCAGT 
GTGTACCGCA TGCAGCAGTA CGACCTGCAG GGGCAAACCT ACGGTTCACG TAATGCCATC 
TTGAACACGG AGGCTCGTAC GGTGGAGGCT GAAGTGCTGA GTCGTCGCTG TGTGATGATG 
CGGCTCGCTG ATTTCTCCTA TGAGAAATAC CAGAAGGCTC TGCGTCAGTC AGCGGGAGCG 
GTGGTCATCA TCCTGCCGCA CAACATGTCC ACCCTACCCC AGGACATAGT GCAGCAGTTC 
ATGGAGCTGG AGCCGGAGCT GTTGGCCACC GAGACCATCG TGCCGGTGTA CTTCGCTCTG 
GAGGACGAGG AGCTGCTGTC TATCTACACA CAGACACAGA TCTCCTCATC CTCACAGGGG 
TCTTCATCCG CCGCTGAAGT GCTGCTTCAT ACGGCTACAG CTAATGGTTT TCAGATGGTG 
ACCAGTGGAG CTCAGAGTAA AGCTGTCAGT GACTGGGCTA TTACTAGTCT AGAGGGCCGC 
CTCACAGGGT CAGGAGGAGA AGATCTGCCC ACCATCGTCC TGGTGGCTCA TTATGACTCT 
TTCGGAGTCG CTCCGTGGCT GTCGTATGGC GCGGACTCAA ACGGCAGTGG CGTCGCTATA 
CTGCTGGAGC TTGCGCGACT GTTTTCCAGA CTGTATTCCT ACAAGAGGAC TCACGCTGGA 
TATAACCTGC TGTTTTTCTT GTCGGGAGGC GGAAAGTTCA ACTATCAAGG CACTAAACGC 
TGGCTAGAGG ACAATCTGGA TCACACAGAT GCCAGTCTGC TCCAGGACAA TGTGGCGTTT 
GTCTTGTGTC TGGACACTCT AGGAAACAGC GATAACCTTC ACCTCCATGT GTCCAAACCA 
CCAAAAGAGG GAAGCCCTCA GTACACCCTG CTCAAAGAGC TAGAAACTGT TGTGGCCCAT 
CAGCACCCCG ACCTGAAGTT CGCGATGGTC CACAAGAAGA TCAACCTGGC GGATGACACT 
CTGGCATGGG AGCACGAGCG CTTCGGGATC CGCCGCCTGC CCGCCTTCAC CCTGTCCCAC 
CTGGAGAGCC ACCGCAGCCC CGCGCGCCAC TCCATCATGG ACATGCGGCC TCATGTGGAT 
CTGGGGAAAC TCAGTCGTAA CACTAAAGTT ATTGCAGAAA CTCTGGCCAG AGTCATATAT 
AACCTGACGG AGAAGGGCGT CACCGGAGAC CTGGAGATCT TCACTGAACA GATGGTGCAG 
GAGGATCAGC TGGCGTCTCT GGTGGACTGG TTGACCGCTC AGCCTCGAGC CGCTCAGCTT 
CTGGATAAAG ACAGCAGCAT CATCAACACG CTGGAGCATC AGCTCAGCCG ATACCTGAAG 
GACGTCAAGA GACACTTGGT GCGAGCAGAC AAGAGAGACC CTGAGTTTGT GTTTTATGAT 
CAGCTCAAGC AGACCATGAA TGCTTACAGG GTAAAACCAG CAATCTTTGA CCTGCTGTTG 
GCCGTCTGTA TCGCATCTTA TCTCGGAGTG CTGTATTTGG CCATTCAGAA TTTTGGACTC 
CTGTATGGTT TTCTGCGCAG AGTCACGGCT CCTCGGGTCA AGCAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161648.1
CDS	143..1813
Translation	MFEEAGEVLENMLKVSFPLSLVLFLVLVCPLRAEAAHEFSVYRMQQYDLQGQTYGSRNAILNTEARTVEAEVLSRRCVMMRLADFSYEKYQKALRQSAGAVVIILPHNMSTLPQDIVQQFMELEPELLATETIVPVYFALEDEELLSIYTQTQISSSSQGSSSAAEVLLHTATANGFQMVTSGAQSKAVSDWAITSLEGRLTGSGGEDLPTIVLVAHYDSFGVAPWLSYGADSNGSGVAILLELARLFSRLYSYKRTHAGYNLLFFLSGGGKFNYQGTKRWLEDNLDHTDASLLQDNVAFVLCLDTLGNSDNLHLHVSKPPKEGSPQYTLLKELETVVAHQHPDLKFAMVHKKINLADDTLAWEHERFGIRRLPAFTLSHLESHRSPARHSIMDMRPHVDLGKLSRNTKVIAETLARVIYNLTEKGVTGDLEIFTEQMVQEDQLASLVDWLTAQPRAAQLLDKDSSIINTLEHQLSRYLKDVKRHLVRADKRDPEFVFYDQLKQTMNAYRVKPAIFDLLLAVCIASYLGVLYLAIQNFGLLYGFLRRVTAPRVKQH

Target ORF information:

RefSeq Version	XM_005161591.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nicalin (ncl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161591.3

ORF Insert Sequence:

ATGTTCGAGG AGGCTGGTGA GGTGTTGGAG AACATGCTGA AGGTGTCGTT CCCGCTCAGC 
CTGGTGCTGT TTCTGGTGCT CGTGTGTCCG CTGCGCGCGG AAGCGGCGCA TGAGTTCAGT 
GTGTACCGCA TGCAGCAGTA CGACCTGCAG GGGCAAACCT ACGGTTCACG TAATGCCATC 
TTGAACACGG AGGCTCGTAC GGTGGAGGCT GAAGTGCTGA GTCGTCGCTG TGTGATGATG 
CGGCTCGCTG ATTTCTCCTA TGAGAAATAC CAGAAGGCTC TGCGTCAGTC AGCGGGAGCG 
GTGGTCATCA TCCTGCCGCA CAACATGTCC ACCCTACCCC AGGACATAGT GCAGCAGTTC 
ATGGAGCTGG AGCCGGAGCT GTTGGCCACC GAGACCATCG TGCCGGTGTA CTTCGCTCTG 
GAGGACGAGG AGCTGCTGTC TATCTACACA CAGACACAGA TCTCCTCATC CTCACAGGGG 
TCTTCATCCG CCGCTGAAGT GCTGCTTCAT ACGGCTACAG CTAATGGTTT TCAGATGGTG 
ACCAGTGGAG CTCAGAGTAA AGCTGTCAGT GACTGGGCTA TTACTAGTCT AGAGGGCCGC 
CTCACAGGGT CAGGAGGAGA AGATCTGCCC ACCATCGTCC TGGTGGCTCA TTATGACTCT 
TTCGGAGTCG CTCCGTGGCT GTCGTATGGC GCGGACTCAA ACGGCAGTGG CGTCGCTATA 
CTGCTGGAGC TTGCGCGACT GTTTTCCAGA CTGTATTCCT ACAAGAGGAC TCACGCTGGA 
TATAACCTGC TGTTTTTCTT GTCGGGAGGC GGAAAGTTCA ACTATCAAGG CACTAAACGC 
TGGCTAGAGG ACAATCTGGA TCACACAGAT GCCAGTCTGC TCCAGGACAA TGTGGCGTTT 
GTCTTGTGTC TGGACACTCT AGGAAACAGC GATAACCTTC ACCTCCATGT GTCCAAACCA 
CCAAAAGAGG GAAGCCCTCA GTACACCCTG CTCAAAGAGC TAGAAACTGT TGTGGCCCAT 
CAGCACCCCG ACCTGAAGTT CGCGATGGTC CACAAGAAGA TCAACCTGGC GGATGACACT 
CTGGCATGGG AGCACGAGCG CTTCGGGATC CGCCGCCTGC CCGCCTTCAC CCTGTCCCAC 
CTGGAGAGCC ACCGCAGCCC CGCGCGCCAC TCCATCATGG ACATGCGGCC TCATGTGGAT 
CTGGGGAAAC TCAGTCGTAA CACTAAAGTT ATTGCAGAAA CTCTGGCCAG AGTCATATAT 
AACCTGACGG AGAAGGGCGT CACCGGAGAC CTGGAGATCT TCACTGAACA GATGGTGCAG 
GAGGATCAGC TGGCGTCTCT GGTGGACTGG TTGACCGCTC AGCCTCGAGC CGCTCAGCTT 
CTGGATAAAG ACAGCAGCAT CATCAACACG CTGGAGCATC AGCTCAGCCG ATACCTGAAG 
GACGTCAAGA GACACTTGGT GCGAGCAGAC AAGAGAGACC CTGAGTTTGT GTTTTATGAT 
CAGCTCAAGC AGACCATGAA TGCTTACAGG GTAAAACCAG CAATCTTTGA CCTGCTGTTG 
GCCGTCTGTA TCGCATCTTA TCTCGGAGTG CTGTATTTGG CCATTCAGAA TTTTGGACTC 
CTGTATGGTT TTCTGCGCAG AGTCACGGCT CCTCGGGTCA AGCAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Whole exome sequencing coupled with unbiased functional analysis reveals new Hirschsprung disease genes.
Genome biology18(1)48(2017 Mar)
Gui H,Schriemer D,Cheng WW,Chauhan RK,Anti?olo G,Berrios C,Bleda M,Brooks AS,Brouwer RW,Burns AJ,Cherny SS,Dopazo J,Eggen BJ,Griseri P,Jalloh B,Le TL,Lui VC,Luz?n-Toro B,Matera I,Ngan ES,Pelet A,Ruiz-Ferrer M,Sham PC,Shepherd IT,So MT,Sribudiani Y,Tang CS,van den Hout MC,van der Linde HC,van Ham TJ,van IJcken WF,Verheij JB,Amiel J,Borrego S,Ceccherini I,Chakravarti A,Lyonnet S,Tam PK,Garcia-Barcel? MM,Hofstra RM

Evolutionary divergence of the vertebrate TNFAIP8 gene family: Applying the spotted gar orthology bridge to understand ohnolog loss in teleosts.
PloS one12(6)e0179517(2017)
Sullivan C,Lage CR,Yoder JA,Postlethwait JH,Kim CH

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Analysis of nicastrin gene phylogeny and expression in zebrafish.
Development genes and evolution225(3)171-8(2015 Jun)
Lim A,Moussavi Nik SH,Ebrahimie E,Lardelli M

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ncln cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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