trmt2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	trmt2a
Entrez Gene ID	334936
Full Name	tRNA methyltransferase 2 homolog A
Synonyms	fa10b06,htf9c,wu:fa10b06,zgc:55519
General protein information	Preferred Names tRNA methyltransferase 2 homolog A Names tRNA (uracil-5-)-methyltransferase homolog A HpaII tiny fragments locus 9c trm2 tRNA methyltransferase 2 homolog A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199929.1	NP_956223.1	tRNA (uracil-5-)-methyltransferase homolog A

Pathways from BioSystems

Homologies

	TRMT2A	XP_002798309.1
Canis lupus familiaris (dog)	TRMT2A	XP_005636628.1
Danio rerio (zebrafish)	trmt2a	NP_956223.1
Caenorhabditis elegans (roundworm)	H24K24.4	NP_503353.2
Homo sapiens (human)	TRMT2A	NP_073564.3
Mus musculus (house mouse)	Trmt2a	NP_001074469.1
Rattus norvegicus (Norway rat)	Trmt2a	NP_001011895.1
Gallus gallus (chicken)	TRMT2A	XP_415080.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012408	XP_320147.4
Xenopus tropicalis (tropical clawed frog)	trmt2a	NP_001120451.2
Pan troglodytes (chimpanzee)	TRMT2A	XP_001167055.1
Bos taurus (cattle)	TRMT2A	XP_002694704.3
Drosophila melanogaster (fruit fly)	CG3808	NP_649083.2

Gene Ontology
Bibliography

Related articles in PubMed

Dynamic RNA-protein interactions underlie the zebrafish maternal-to-zygotic transition.
Despic V, Dejung M, Gu M, Krishnan J, Zhang J, Herzel L, Straube K, Gerstein MB, Butter F, Neugebauer KM
Genome research27(7)1184-1194(2017 Jul)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Comparative mapping of the 22q11.2 deletion region and the potential of simple model organisms.
Guna A, Butcher NJ, Bassett AS
Journal of neurodevelopmental disorders7(1)18(2015)

MicroRNA-3906 regulates fast muscle differentiation through modulating the target gene homer-1b in zebrafish embryos.
Lin CY, Chen JS, Loo MR, Hsiao CC, Chang WY, Tsai HJ
PloS one8(7)e70187(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following trmt2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trmt2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03896	NM_199929.1 Latest version!	Danio rerio tRNA methyltransferase 2 homolog A (trmt2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trmt2a ( NM_199929.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956223.1 Danio rerio tRNA methyltransferase 2 homolog A (trmt2a), mRNA.

Price & Availability↑

CloneID	ODa03896
Clone ID Related Accession (Same CDS sequence)	NM_199929.1
Accession Version	NM_199929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-26
Organism	Danio rerio(zebrafish)
Product	tRNA (uracil-5-)-methyltransferase homolog A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC045367.1. ##Evidence-Data-START## Transcript exon combination :: BC045367.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGACAGATG TTGCCGTTGA CCAGAATGAT CCAGCTGGTT TATTACCCAG TGCAGAGGAG 
AAACCAGAAG AACTGACGGA GGAGAAAATC CAAGAGAACG AGCCAGAAGA CTCGACAGAG 
AAGAAAAACC AAGAGAATGA GCCAGAAGCA GCAGAAGGAC AGGCTGCAGC AGGGGATGTT 
TACCGCTACA TCAAGGAGGA TCTCTTCACC TCCGAGATCT ATAAAGTGGA GATCCAGAAC 
CTGCCCAAGT ACATTGGATT TAACGACCTG AAGAAGTTCC TCAACAAGCA TGGGATTAAT 
CCTCACAAAA TCAAGCTGCT CGGCAGACAA AAGTTTGCCT TTGTCACTTT TAAGAACCAG 
GAGGAAAGGG ACAAGGCCAT GAAGGCAGTG CATGGCATGC AGTGGAAAAG TAATGTGCTG 
AGTGTTCGGC TGGCCAAGCC CAAAGCAGAT CCCATCCTCA AGAAGAGGAA ACAGGAGGAG 
GAGGCGGACA GCGGGCAACC TGGTGCCGAG CGAAATACAG GGAGCCAGGA GGCAGAAAAG 
GAGGAGGAAG AGCCTCCCAG TATACAGATT GCCAATGCCG TGACCCCATT GTGGAAAGTG 
CCTTATGAAG AACAGCTGAT GATGAAAGAG AAAGAAGTCG AAGGTGTTCT CCAAAAACTC 
GCCAGAGAAA TTGGCAACAA CAACAAAGCC ATGCTTCCGT GGTTGTTCGT CCAAAAAGAA 
AAACACAACA AAATGTGTTG TCCACTCGAA GCCATACGAC CATCACCCCT GCAGACGGGG 
TACAGGAATA AGTGCGAGTT TGTGATTGGA GTGGGAGCAG ACGGAGAGGA CAGGACTGTA 
GGCTTTCGTT TGGGCAAGTA TAAAGGAGGA TCCTGTGCAG TGGTCAGTCC ATCAGAAACC 
ACCAATGTGT GTTCTGAGGC CAAGAGGGTT GTGCAGTGTT TCCAGCAGTT CATCAGGACA 
ACTCCATATG CAGTGTACAG TCCCGAGACT TATGAGGGTC ATTGGCGGCA GCTGACGGTA 
CGGACAACCC GATTAAAGCA AACTATGGCC ATGGTTTTCT TCAATCCTCA GAAACTTCAA 
GAGAAAGAAC TTGAGGAGCT GAAACAGAAC CTGGGTCAGT ATTTCACAGA AGGAGAAGGA 
AAAGAATGTG CTGTTACTTC TTTGTACTTT GTGAGAATGG GACAAAGAAC ATCTGCGGGC 
ACAGAGGATT TGCCATGTGA ACATGTGACT GGAGAAGAGT GGATTCATGA GGAGCTTCTC 
GGACTCAAAT TCCGCATTTC TCCACACTCA TTCTTCCAGA CAAACACACC TGCTGCTGAG 
ATCCTGTACT CGGCCGTGGG TGAATGGGCT CAGCTGGACC AGGACAGCAC TGTGCTAGAT 
GTGTGCTGTG GAACCGGAAC CATCGGCATC TCACTGGCAA AGAGGGTGAA GAAGGTGATT 
GGCATAGAGT TGTGCCAAGA GGCAGTAAAA GATGCTGAGG CTAATGCTAA AGCTAACGGT 
TTGACCAATG TGGAGTTTCA CTGTGGAAAG GCTGAGGACG TGTTTCCCAC TATCCTTAAT 
GCTGTCGTTT CCCCAAATGT CACCGCAATC GTCGACCCTC CGAGGGCAGG CCTACATTCC 
AAAGTTATTC TTGCAATCAG AAAAGCAGAG CATCTGAAGA GACTGGTCTA TGTCGCCTGC 
AATGCCAAAG CAGCGATGAA CAATTTTATT GATCTCTGCA GAGCTCCCTC AAACCGTGTG 
CGAGGAGCTG CATTTCGTCC AGTGAAAGCG ATGGCTGTGG ACCTCTTCCC TCAGACAATG 
CACTGTGAAA CCATCCTGCT GTTTGAAAGA GTGGACTACA GCACCAACAC TGACGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956223.1
CDS	84..1943
Translation	MTDVAVDQNDPAGLLPSAEEKPEELTEEKIQENEPEDSTEKKNQENEPEAAEGQAAAGDVYRYIKEDLFTSEIYKVEIQNLPKYIGFNDLKKFLNKHGINPHKIKLLGRQKFAFVTFKNQEERDKAMKAVHGMQWKSNVLSVRLAKPKADPILKKRKQEEEADSGQPGAERNTGSQEAEKEEEEPPSIQIANAVTPLWKVPYEEQLMMKEKEVEGVLQKLAREIGNNNKAMLPWLFVQKEKHNKMCCPLEAIRPSPLQTGYRNKCEFVIGVGADGEDRTVGFRLGKYKGGSCAVVSPSETTNVCSEAKRVVQCFQQFIRTTPYAVYSPETYEGHWRQLTVRTTRLKQTMAMVFFNPQKLQEKELEELKQNLGQYFTEGEGKECAVTSLYFVRMGQRTSAGTEDLPCEHVTGEEWIHEELLGLKFRISPHSFFQTNTPAAEILYSAVGEWAQLDQDSTVLDVCCGTGTIGISLAKRVKKVIGIELCQEAVKDAEANAKANGLTNVEFHCGKAEDVFPTILNAVVSPNVTAIVDPPRAGLHSKVILAIRKAEHLKRLVYVACNAKAAMNNFIDLCRAPSNRVRGAAFRPVKAMAVDLFPQTMHCETILLFERVDYSTNTDV

Target ORF information:

RefSeq Version	NM_199929.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tRNA methyltransferase 2 homolog A (trmt2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199929.1

ORF Insert Sequence:

ATGACAGATG TTGCCGTTGA CCAGAATGAT CCAGCTGGTT TATTACCCAG TGCAGAGGAG 
AAACCAGAAG AACTGACGGA GGAGAAAATC CAAGAGAACG AGCCAGAAGA CTCGACAGAG 
AAGAAAAACC AAGAGAATGA GCCAGAAGCA GCAGAAGGAC AGGCTGCAGC AGGGGATGTT 
TACCGCTACA TCAAGGAGGA TCTCTTCACC TCCGAGATCT ATAAAGTGGA GATCCAGAAC 
CTGCCCAAGT ACATTGGATT TAACGACCTG AAGAAGTTCC TCAACAAGCA TGGGATTAAT 
CCTCACAAAA TCAAGCTGCT CGGCAGACAA AAGTTTGCCT TTGTCACTTT TAAGAACCAG 
GAGGAAAGGG ACAAGGCCAT GAAGGCAGTG CATGGCATGC AGTGGAAAAG TAATGTGCTG 
AGTGTTCGGC TGGCCAAGCC CAAAGCAGAT CCCATCCTCA AGAAGAGGAA ACAGGAGGAG 
GAGGCGGACA GCGGGCAACC TGGTGCCGAG CGAAATACAG GGAGCCAGGA GGCAGAAAAG 
GAGGAGGAAG AGCCTCCCAG TATACAGATT GCCAATGCCG TGACCCCATT GTGGAAAGTG 
CCTTATGAAG AACAGCTGAT GATGAAAGAG AAAGAAGTCG AAGGTGTTCT CCAAAAACTC 
GCCAGAGAAA TTGGCAACAA CAACAAAGCC ATGCTTCCGT GGTTGTTCGT CCAAAAAGAA 
AAACACAACA AAATGTGTTG TCCACTCGAA GCCATACGAC CATCACCCCT GCAGACGGGG 
TACAGGAATA AGTGCGAGTT TGTGATTGGA GTGGGAGCAG ACGGAGAGGA CAGGACTGTA 
GGCTTTCGTT TGGGCAAGTA TAAAGGAGGA TCCTGTGCAG TGGTCAGTCC ATCAGAAACC 
ACCAATGTGT GTTCTGAGGC CAAGAGGGTT GTGCAGTGTT TCCAGCAGTT CATCAGGACA 
ACTCCATATG CAGTGTACAG TCCCGAGACT TATGAGGGTC ATTGGCGGCA GCTGACGGTA 
CGGACAACCC GATTAAAGCA AACTATGGCC ATGGTTTTCT TCAATCCTCA GAAACTTCAA 
GAGAAAGAAC TTGAGGAGCT GAAACAGAAC CTGGGTCAGT ATTTCACAGA AGGAGAAGGA 
AAAGAATGTG CTGTTACTTC TTTGTACTTT GTGAGAATGG GACAAAGAAC ATCTGCGGGC 
ACAGAGGATT TGCCATGTGA ACATGTGACT GGAGAAGAGT GGATTCATGA GGAGCTTCTC 
GGACTCAAAT TCCGCATTTC TCCACACTCA TTCTTCCAGA CAAACACACC TGCTGCTGAG 
ATCCTGTACT CGGCCGTGGG TGAATGGGCT CAGCTGGACC AGGACAGCAC TGTGCTAGAT 
GTGTGCTGTG GAACCGGAAC CATCGGCATC TCACTGGCAA AGAGGGTGAA GAAGGTGATT 
GGCATAGAGT TGTGCCAAGA GGCAGTAAAA GATGCTGAGG CTAATGCTAA AGCTAACGGT 
TTGACCAATG TGGAGTTTCA CTGTGGAAAG GCTGAGGACG TGTTTCCCAC TATCCTTAAT 
GCTGTCGTTT CCCCAAATGT CACCGCAATC GTCGACCCTC CGAGGGCAGG CCTACATTCC 
AAAGTTATTC TTGCAATCAG AAAAGCAGAG CATCTGAAGA GACTGGTCTA TGTCGCCTGC 
AATGCCAAAG CAGCGATGAA CAATTTTATT GATCTCTGCA GAGCTCCCTC AAACCGTGTG 
CGAGGAGCTG CATTTCGTCC AGTGAAAGCG ATGGCTGTGG ACCTCTTCCC TCAGACAATG 
CACTGTGAAA CCATCCTGCT GTTTGAAAGA GTGGACTACA GCACCAACAC TGACGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dynamic RNA-protein interactions underlie the zebrafish maternal-to-zygotic transition.
Genome research27(7)1184-1194(2017 Jul)
Despic V,Dejung M,Gu M,Krishnan J,Zhang J,Herzel L,Straube K,Gerstein MB,Butter F,Neugebauer KM

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Comparative mapping of the 22q11.2 deletion region and the potential of simple model organisms.
Journal of neurodevelopmental disorders7(1)18(2015)
Guna A,Butcher NJ,Bassett AS

MicroRNA-3906 regulates fast muscle differentiation through modulating the target gene homer-1b in zebrafish embryos.
PloS one8(7)e70187(2013)
Lin CY,Chen JS,Loo MR,Hsiao CC,Chang WY,Tsai HJ