hhip cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	hhip
Entrez Gene ID	326102
Full Name	hedgehog interacting protein
Synonyms	fd99f01,wu:fc52e12,wu:fd99f01,zgc:153478
General protein information	Preferred Names hedgehog interacting protein Names hedgehog-interacting protein Hip uki ukkie
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001080012.1	NP_001073481.1	hedgehog-interacting protein

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	HHIP	XP_420424.4
Danio rerio (zebrafish)	hhip	NP_001073481.1
Homo sapiens (human)	HHIP	NP_071920.1
	HHIP	XP_001093950.1
Rattus norvegicus (Norway rat)	Hhip	NP_001178746.1
Pan troglodytes (chimpanzee)	HHIP	XP_001146476.1
Canis lupus familiaris (dog)	HHIP	XP_003639584.1
Mus musculus (house mouse)	Hhip	NP_064655.4
Xenopus tropicalis (tropical clawed frog)	hhip	NP_001007191.1
Bos taurus (cattle)	HHIP	NP_001092584.1

Gene Ontology
Bibliography

Related articles in PubMed

UbcD1 regulates Hedgehog signaling by directly modulating Ci ubiquitination and processing.
Pan C, Xiong Y, Lv X, Xia Y, Zhang S, Chen H, Fan J, Wu W, Liu F, Wu H, Zhou Z, Zhang L, Zhao Y
EMBO reports18(11)1922-1934(2017 Nov)

Regulation of Gli ciliary localization and Hedgehog signaling by the PY-NLS/karyopherin-?2 nuclear import system.
Han Y, Xiong Y, Shi X, Wu J, Zhao Y, Jiang J
PLoS biology15(8)e2002063(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hhip regulates muscle development both by sequestering Hedgehog and by modulating localization of Smoothened
Title: Hhip regulates zebrafish muscle development by both sequestering Hedgehog and modulating localization of Smoothened.

The following hhip gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hhip cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01769	NM_001080012.1 Latest version!	Danio rerio hedgehog interacting protein (hhip), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hhip ( NM_001080012.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001073481.1 Danio rerio hedgehog interacting protein (hhip), mRNA.

Price & Availability↑

CloneID	ODa01769
Clone ID Related Accession (Same CDS sequence)	NM_001080012.1
Accession Version	NM_001080012.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-14
Organism	Danio rerio(zebrafish)
Product	hedgehog-interacting protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC128659.1. On Mar 11, 2007 this sequence version replaced XM_687503.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC128659.1, DQ177323.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476791, SAMEA708828 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCATT TGAAATTTGT GCTCTTGTTA GCCATCGCGG CCAATTTTTG GGAATGTGGT 
GATGCGAAAT TTGGCGAGAA AGGAGAATTA AGTCCAAGGA GGAGGAGATG TTACGATGGA 
AGTCTGCCGA GACGCCTGAA GAAGAAGGAG AGAAAACTGT CACTCGAGGG AAGCGGTGGA 
GGGGAGGTTT GTCACAGGCT CTATCCTCGC GTGTCCTGCT GTCCGTCCAG GAGGGCTCCG 
TATCAGATAC TGCATCGGAA AGATGCTCGG ACTTTCTCGA CTAATAACAC GGAGTGTTCT 
AGACTGCTGG AGGAGATTAA GTGTGCCCAT TGCTCACCAA ATGCTCAGAT GCTGTTCCAT 
TCACCTAAAC TTGAAAAAGC ACCACATAGA GAACAAGACC TACCCCACTT ATGCCATGAC 
TACTGCCAAG AGTTCTACTA CACCTGCCGT GGACACGTAC CAGAACTGTT CCAAGCCGAC 
GTGGATGAGT TCTGCCAGTA CTATGGAAGG ATGGATGGAG GCCTGTGCTT TCCAGATTTT 
CACCGAAAGC AATTGCGGAG AGATTCCAAC TACCTGTTAG ATGAAAAAAC CGAAGCGATT 
AACAGAAAAC ACAAACACAA CTGCTACTGC GCTCAGGAGA TCCATAGTGG TCTTCAACAA 
CCTGTTGGCG TGGTGCATTG TGGGGATGGA TCGCAGCGGC TTTTTATATT GGAGAGGGAA 
GGCTTTGTGT GGATCCTCAC ACATGACATG GAACTCCTAA AAGAGCCTTT TCTGGACATT 
CATAAGCTGG TACAAAGTGG TTTAAAGGGG GGAGATGAAA GGGGCTTGCT AAGCCTTGCA 
TTCCACCCCA ATTATAAGAA AAATGGCAAG CTCTACGTCT CCTATACGAC CAACCAGGAG 
CGATGGACTA TTGGACCACA CGACCACATT CTTCGTGTAG TGGAGTACAC AGTGTCCAGA 
AAAAATCCAA ACCAGGTGGA CACAAGGACT CCTCGGGTTT TAATGGAAGT TGCGGAACTT 
CACCGAAAGC ATCTGGGAGG CCAGCTCCTC TTTGGGCCTG ATGGGCTTCT GCACATCTTT 
TTAGGAGATG GCATGATCAC TTTGGACAAT ATGGAGGAGA TGGATGGTCT GAGTGATTTC 
ACAGGTTCTG TTCTTCGGGT GGATGTGGAC ACAGAATGTT GTAGTACTCC CTACTCCATA 
CCCAGAAACA ATCCCTATTT TAACAGCACA AATCAACCCC CCGAAATTTT TGCCCATGGT 
CTGCATGACC CAGGGAGGTG TGCAGTAGAT AAGCTCCGCA TGGACACCAA TGGGAGTCTG 
CTGATCCTGT GCACAGATAC AGTTGGCAAA AATACGACAA CAGGCAGGAT CCTACAGGTC 
ATCAAAGGGA AAGATTACGA AAACGAGCCA TCTATGTTTG ACTTGGGGTC AAGCGGAGGT 
ACCACCCCTG TTGGTGGATT TATCTACAGA GGATGTCAGT CAAGAAGACT TTACGGAAGT 
TATGTATTTG GAGACAAAAA TGGGAACTTT AGAATTCTCC AGAGGCCTTT AGAAGACCGA 
TTGTGGCAAG AGAAGCCTCT TTGTCTTGGT ACTAGCAGTT CCTGTGGTTC CTCGCTGGTA 
GGCCACATCC TGGGGTTTGG CGAAGATGAA TTAGGTGAGG TCTACATCCT TGTCTCCAGC 
AAGAGCACAG CCAAACAGTC GCATGGAAAG ATCTACAAGT TGGTGGACCC CAAAAGACCA 
CAAGTTCCAA AGGAGTGCAG AAGACCAGTA GAAGATCCAG AGATGCTAAG CACTGCTTGT 
TCACGTGAAT GCAAGAACGG CCACTGTACA CCAACTGGCA AGTGCTGCTG CAATGCAGGC 
TGGGAGGGCC CCTTCTGCTT ACGAGCCAAG TGTGAACTGG CTTGTCGCAA TGGCGGGGTC 
TGTGTGGAGC CCAACAAGTG TCTCTGCAAG GAAGGTTTTT CTGGCAACCA GTGCAGTAAA 
GGAGAGCGAG GGACAAAAGG GGACGGTGAG AAAGACAGCA TCCTGGAGCA CATCATTGAC 
ATGACGACTT ACCTGCTGGA CCTCACTAGT TATATTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073481.1
CDS	406..2487
Translation	MKHLKFVLLLAIAANFWECGDAKFGEKGELSPRRRRCYDGSLPRRLKKKERKLSLEGSGGGEVCHRLYPRVSCCPSRRAPYQILHRKDARTFSTNNTECSRLLEEIKCAHCSPNAQMLFHSPKLEKAPHREQDLPHLCHDYCQEFYYTCRGHVPELFQADVDEFCQYYGRMDGGLCFPDFHRKQLRRDSNYLLDEKTEAINRKHKHNCYCAQEIHSGLQQPVGVVHCGDGSQRLFILEREGFVWILTHDMELLKEPFLDIHKLVQSGLKGGDERGLLSLAFHPNYKKNGKLYVSYTTNQERWTIGPHDHILRVVEYTVSRKNPNQVDTRTPRVLMEVAELHRKHLGGQLLFGPDGLLHIFLGDGMITLDNMEEMDGLSDFTGSVLRVDVDTECCSTPYSIPRNNPYFNSTNQPPEIFAHGLHDPGRCAVDKLRMDTNGSLLILCTDTVGKNTTTGRILQVIKGKDYENEPSMFDLGSSGGTTPVGGFIYRGCQSRRLYGSYVFGDKNGNFRILQRPLEDRLWQEKPLCLGTSSSCGSSLVGHILGFGEDELGEVYILVSSKSTAKQSHGKIYKLVDPKRPQVPKECRRPVEDPEMLSTACSRECKNGHCTPTGKCCCNAGWEGPFCLRAKCELACRNGGVCVEPNKCLCKEGFSGNQCSKGERGTKGDGEKDSILEHIIDMTTYLLDLTSYIV

Target ORF information:

RefSeq Version	NM_001080012.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio hedgehog interacting protein (hhip), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080012.1

ORF Insert Sequence:

ATGAAGCATT TGAAATTTGT GCTCTTGTTA GCCATCGCGG CCAATTTTTG GGAATGTGGT 
GATGCGAAAT TTGGCGAGAA AGGAGAATTA AGTCCAAGGA GGAGGAGATG TTACGATGGA 
AGTCTGCCGA GACGCCTGAA GAAGAAGGAG AGAAAACTGT CACTCGAGGG AAGCGGTGGA 
GGGGAGGTTT GTCACAGGCT CTATCCTCGC GTGTCCTGCT GTCCGTCCAG GAGGGCTCCG 
TATCAGATAC TGCATCGGAA AGATGCTCGG ACTTTCTCGA CTAATAACAC GGAGTGTTCT 
AGACTGCTGG AGGAGATTAA GTGTGCCCAT TGCTCACCAA ATGCTCAGAT GCTGTTCCAT 
TCACCTAAAC TTGAAAAAGC ACCACATAGA GAACAAGACC TACCCCACTT ATGCCATGAC 
TACTGCCAAG AGTTCTACTA CACCTGCCGT GGACACGTAC CAGAACTGTT CCAAGCCGAC 
GTGGATGAGT TCTGCCAGTA CTATGGAAGG ATGGATGGAG GCCTGTGCTT TCCAGATTTT 
CACCGAAAGC AATTGCGGAG AGATTCCAAC TACCTGTTAG ATGAAAAAAC CGAAGCGATT 
AACAGAAAAC ACAAACACAA CTGCTACTGC GCTCAGGAGA TCCATAGTGG TCTTCAACAA 
CCTGTTGGCG TGGTGCATTG TGGGGATGGA TCGCAGCGGC TTTTTATATT GGAGAGGGAA 
GGCTTTGTGT GGATCCTCAC ACATGACATG GAACTCCTAA AAGAGCCTTT TCTGGACATT 
CATAAGCTGG TACAAAGTGG TTTAAAGGGG GGAGATGAAA GGGGCTTGCT AAGCCTTGCA 
TTCCACCCCA ATTATAAGAA AAATGGCAAG CTCTACGTCT CCTATACGAC CAACCAGGAG 
CGATGGACTA TTGGACCACA CGACCACATT CTTCGTGTAG TGGAGTACAC AGTGTCCAGA 
AAAAATCCAA ACCAGGTGGA CACAAGGACT CCTCGGGTTT TAATGGAAGT TGCGGAACTT 
CACCGAAAGC ATCTGGGAGG CCAGCTCCTC TTTGGGCCTG ATGGGCTTCT GCACATCTTT 
TTAGGAGATG GCATGATCAC TTTGGACAAT ATGGAGGAGA TGGATGGTCT GAGTGATTTC 
ACAGGTTCTG TTCTTCGGGT GGATGTGGAC ACAGAATGTT GTAGTACTCC CTACTCCATA 
CCCAGAAACA ATCCCTATTT TAACAGCACA AATCAACCCC CCGAAATTTT TGCCCATGGT 
CTGCATGACC CAGGGAGGTG TGCAGTAGAT AAGCTCCGCA TGGACACCAA TGGGAGTCTG 
CTGATCCTGT GCACAGATAC AGTTGGCAAA AATACGACAA CAGGCAGGAT CCTACAGGTC 
ATCAAAGGGA AAGATTACGA AAACGAGCCA TCTATGTTTG ACTTGGGGTC AAGCGGAGGT 
ACCACCCCTG TTGGTGGATT TATCTACAGA GGATGTCAGT CAAGAAGACT TTACGGAAGT 
TATGTATTTG GAGACAAAAA TGGGAACTTT AGAATTCTCC AGAGGCCTTT AGAAGACCGA 
TTGTGGCAAG AGAAGCCTCT TTGTCTTGGT ACTAGCAGTT CCTGTGGTTC CTCGCTGGTA 
GGCCACATCC TGGGGTTTGG CGAAGATGAA TTAGGTGAGG TCTACATCCT TGTCTCCAGC 
AAGAGCACAG CCAAACAGTC GCATGGAAAG ATCTACAAGT TGGTGGACCC CAAAAGACCA 
CAAGTTCCAA AGGAGTGCAG AAGACCAGTA GAAGATCCAG AGATGCTAAG CACTGCTTGT 
TCACGTGAAT GCAAGAACGG CCACTGTACA CCAACTGGCA AGTGCTGCTG CAATGCAGGC 
TGGGAGGGCC CCTTCTGCTT ACGAGCCAAG TGTGAACTGG CTTGTCGCAA TGGCGGGGTC 
TGTGTGGAGC CCAACAAGTG TCTCTGCAAG GAAGGTTTTT CTGGCAACCA GTGCAGTAAA 
GGAGAGCGAG GGACAAAAGG GGACGGTGAG AAAGACAGCA TCCTGGAGCA CATCATTGAC 
ATGACGACTT ACCTGCTGGA CCTCACTAGT TATATTGTTT AA