dvl2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dvl2
Entrez Gene ID	323630
Full Name	dishevelled segment polarity protein 2
Synonyms	sb:eu471,wu:fc05d12,wu:fo71e09,wu:fp54a02,zgc:55372
General protein information	Preferred Names dishevelled segment polarity protein 2 Names segment polarity protein dishevelled homolog DVL-2 dishevelled 2, dsh homolog dishevelled, dsh homolog 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_212648.1	NP_997813.1	segment polarity protein dishevelled homolog DVL-2

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	dsh-1	NP_494978.4
Pan troglodytes (chimpanzee)	DVL2	XP_003315389.1
Bos taurus (cattle)	DVL2	NP_001178311.1
Mus musculus (house mouse)	Dvl2	NP_031914.3
Rattus norvegicus (Norway rat)	Dvl2	NP_001165527.1
Homo sapiens (human)	DVL2	NP_004413.1
Canis lupus familiaris (dog)	DVL2	XP_546582.2
	DVL2	XP_001106375.1
Danio rerio (zebrafish)	dvl2	NP_997813.1
Caenorhabditis elegans (roundworm)	dsh-2	NP_494937.3
Xenopus tropicalis (tropical clawed frog)	dvl2	NP_001072660.1

Gene Ontology
Bibliography

Related articles in PubMed

Leucine repeat adaptor protein 1 interacts with Dishevelled to regulate gastrulation cell movements in zebrafish.
Cheng XN, Shao M, Li JT, Wang YF, Qi J, Xu ZG, Shi DL
Nature communications8(1)1353(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

demonstrate that during early zebrafish development Nkd1 does not alter either endogenous or exogenous levels of Dvl2
Title: Dishevelled2 is a stable protein during early zebrafish development.

The following dvl2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dvl2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03443	NM_212648.1 Latest version!	Danio rerio dishevelled segment polarity protein 2 (dvl2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dvl2 ( NM_212648.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_997813.1 Danio rerio dishevelled segment polarity protein 2 (dvl2), mRNA.

Price & Availability↑

CloneID	ODa03443
Clone ID Related Accession (Same CDS sequence)	NM_212648.1
Accession Version	NM_212648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-23
Organism	Danio rerio(zebrafish)
Product	segment polarity protein dishevelled homolog DVL-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC044381.1. On Jul 6, 2005 this sequence version replaced XM_697465.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC044381.1, AY552332.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGA CCAAGATAAT TTATCACATT GACGAAGAGG AGACGCCGTA CTTGGTGAAG 
ATTCCCATAG CGGCCGAGAA AATCACTTTG CTGGATTTTA AGCAGGTCTT GAACAAACCG 
AACTACAAAT TCTTCTTCAA GTCTATGGAC CAGGACTTCG GGGTGGTGAA GGAAGAGATC 
TCAGATGACA GTGCCAAGCT GCCATGCTTC AACGGAAGAG TTGTCTCTTG GCTGGTGTCC 
TCGGATACCC CTGCAGCAGA GCCTGTAGCT CCTCCAGTAG AGGTCCCGTC TCAGCCCTCA 
CCTCCTCCTC CACCCCTGCC TCCACCTCCT GCTGAGAGGA TGGCGGGCAT TGGAGACTCC 
AGACCTCCTT CTTTCCATCC CAACACCACA GGCAGCCTGG AGTCTTTAGA TGATCAGACC 
GAGACGGAGT CAGTCGTGTC TTTCAGGAGA GAGAGACCTC GACCCAGAGA GAGCTTGGAG 
CAACATGGGC CCCGTATGAA TGGACAGTCC CATTTGGACA GACACCTGGC CGGATATGAG 
AGCGCTACTA CAGTGATGAG CAGTGAACTG GACACCACAA GTTTTTGTGA CTCTGATGAC 
GATGATACCA TGAGCAGGTT CAGCAGCTCC ACTGAACAGA GCACTGCATC TAGATTGCTC 
AAACGCCACC GAAGACGCCG GAAACAGCGG CCGCGACTGG AAAGGGCCTC CTCTTTCAGC 
AGTGTGACTG ATTCAACCAT GTCCCTCAAC ATTATTACAG TCACTCTTAA CATGGAGAAG 
TATAATTTCT TGGGCATCAG TATCGTGGGT CAGAGCAATG AGAGAGGAGA TGGAGGAATC 
TACATTGGCT CCATTATGAA GGGAGGAGCT GTAGCTGCAG ATGGACGTAT TGAGCCTGGA 
GACATGCTGC TGCAGGTGAA TGATATAAAC TTTGAGAACA TGAGTAATGA TGATGCGGTC 
CGGGTGCTGC GTGAGATTGT CCACAAACCT GGACCCATCA CCCTAACTGT GGCCAAATGT 
TGGGACCCTT CTCCACAAGG GTATTTCACC CTGCCACGCA ATGAGCCAAT CCGGCCCATA 
GACCCAGCAG CTTGGGTCAA TCACTCAGTG GCTCTGACAG GGGCGTTTCC GTCCTATCCC 
AGCAGCACAA TCACATCCAG CTCATCCGTC ACCGAAACCG AACGCTTTGA TGAGTTTAAC 
CTGTCTCTTC GCTCAGACAT GGCCTCGGTC GCTAAAGCCA TGGCATCTCC AGAGTCCGGT 
CTAGAAGTCA GAGATCGAAT GTGGCTGAAG ATCACCATTC CCAATGCTTT TCTGGGTTCA 
GATGTGGTGG AGTGGTTGTA CCATCACATT GATGGCTTTC AGGACAGACG CGAAGCTCGG 
AAATATGCCT GTAACTTACT AAAGGCTGGT TTCATCAGGC ACACTGTCAA CAAGATCACC 
TTCTCTGAAC AGTGCTACTA CATATTTGGA GACTTCAGCA ACTGTGAAAA CTACATGGCC 
AACCTTTCTC TCAATGACAA TGATGGATCC AGCGGTGCTT CAGACCAGGA CACTCTGGCT 
CCTCTTCCTC TGCCAGGGGC GACACCTTGG CCTCTGCTCC ACTCGTTCAC ATACCAGTAC 
CCTCATCCTT ACTCCACCCA GCCTCCGCCA TACCATGAGC TCTCTAACTA CAGCTACGGC 
CCAGGCAGTG CAGGCAGTCA GCATAGTGAA GGGAGTCACA GTAGTGGCTC CACACGCAGC 
GATGGAGAAA AGAGAAGGGG CCCTAAAAGC GTTTCTGAGA GTACTGTGGG CGGCAGCGTG 
CGTGATGATA AATCTCCTGG CGGGGGTGGA GCTGACTCAC GTTCTGGCAG CGGCAGCGAA 
TCAGATTACT CTGTTCGCAG CACCCTGAGA CGAGATCATG GCTCAGCCAC ACCCAGCGAA 
CACAGCCGCT CGAGTCAGCG CTCGCATCAC CGCGTCCCGC CCCCTCACCT CGCCCCGTAT 
CCGCCCGGCA TCCCCATCCC ATACAACCCA ATGATGGTGA TGATGGTGCC CCAACACCCA 
CATCTGGCGC TTGGAGCACC ACATCCGCAA ACACCTACTC TGCCGCCCCA CCCTGGCTTA 
CCCCCAACCG GCATCCCCGG CGGGCCTCCA GGAGCTCCCC CGACCCGAGA CCTGGGCTCC 
GTCCCGCCTG AGCTGACTGC TTCCAGGCAG TCCTTCCACC TGGCCATGGG CAACCCCAGT 
GAGTTTTTTG TGGATGTGAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997813.1
CDS	80..2323
Translation	MAETKIIYHIDEEETPYLVKIPIAAEKITLLDFKQVLNKPNYKFFFKSMDQDFGVVKEEISDDSAKLPCFNGRVVSWLVSSDTPAAEPVAPPVEVPSQPSPPPPPLPPPPAERMAGIGDSRPPSFHPNTTGSLESLDDQTETESVVSFRRERPRPRESLEQHGPRMNGQSHLDRHLAGYESATTVMSSELDTTSFCDSDDDDTMSRFSSSTEQSTASRLLKRHRRRRKQRPRLERASSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDINFENMSNDDAVRVLREIVHKPGPITLTVAKCWDPSPQGYFTLPRNEPIRPIDPAAWVNHSVALTGAFPSYPSSTITSSSSVTETERFDEFNLSLRSDMASVAKAMASPESGLEVRDRMWLKITIPNAFLGSDVVEWLYHHIDGFQDRREARKYACNLLKAGFIRHTVNKITFSEQCYYIFGDFSNCENYMANLSLNDNDGSSGASDQDTLAPLPLPGATPWPLLHSFTYQYPHPYSTQPPPYHELSNYSYGPGSAGSQHSEGSHSSGSTRSDGEKRRGPKSVSESTVGGSVRDDKSPGGGGADSRSGSGSESDYSVRSTLRRDHGSATPSEHSRSSQRSHHRVPPPHLAPYPPGIPIPYNPMMVMMVPQHPHLALGAPHPQTPTLPPHPGLPPTGIPGGPPGAPPTRDLGSVPPELTASRQSFHLAMGNPSEFFVDVM

Target ORF information:

RefSeq Version	NM_212648.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dishevelled segment polarity protein 2 (dvl2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212648.1

ORF Insert Sequence:

ATGGCGGAGA CCAAGATAAT TTATCACATT GACGAAGAGG AGACGCCGTA CTTGGTGAAG 
ATTCCCATAG CGGCCGAGAA AATCACTTTG CTGGATTTTA AGCAGGTCTT GAACAAACCG 
AACTACAAAT TCTTCTTCAA GTCTATGGAC CAGGACTTCG GGGTGGTGAA GGAAGAGATC 
TCAGATGACA GTGCCAAGCT GCCATGCTTC AACGGAAGAG TTGTCTCTTG GCTGGTGTCC 
TCGGATACCC CTGCAGCAGA GCCTGTAGCT CCTCCAGTAG AGGTCCCGTC TCAGCCCTCA 
CCTCCTCCTC CACCCCTGCC TCCACCTCCT GCTGAGAGGA TGGCGGGCAT TGGAGACTCC 
AGACCTCCTT CTTTCCATCC CAACACCACA GGCAGCCTGG AGTCTTTAGA TGATCAGACC 
GAGACGGAGT CAGTCGTGTC TTTCAGGAGA GAGAGACCTC GACCCAGAGA GAGCTTGGAG 
CAACATGGGC CCCGTATGAA TGGACAGTCC CATTTGGACA GACACCTGGC CGGATATGAG 
AGCGCTACTA CAGTGATGAG CAGTGAACTG GACACCACAA GTTTTTGTGA CTCTGATGAC 
GATGATACCA TGAGCAGGTT CAGCAGCTCC ACTGAACAGA GCACTGCATC TAGATTGCTC 
AAACGCCACC GAAGACGCCG GAAACAGCGG CCGCGACTGG AAAGGGCCTC CTCTTTCAGC 
AGTGTGACTG ATTCAACCAT GTCCCTCAAC ATTATTACAG TCACTCTTAA CATGGAGAAG 
TATAATTTCT TGGGCATCAG TATCGTGGGT CAGAGCAATG AGAGAGGAGA TGGAGGAATC 
TACATTGGCT CCATTATGAA GGGAGGAGCT GTAGCTGCAG ATGGACGTAT TGAGCCTGGA 
GACATGCTGC TGCAGGTGAA TGATATAAAC TTTGAGAACA TGAGTAATGA TGATGCGGTC 
CGGGTGCTGC GTGAGATTGT CCACAAACCT GGACCCATCA CCCTAACTGT GGCCAAATGT 
TGGGACCCTT CTCCACAAGG GTATTTCACC CTGCCACGCA ATGAGCCAAT CCGGCCCATA 
GACCCAGCAG CTTGGGTCAA TCACTCAGTG GCTCTGACAG GGGCGTTTCC GTCCTATCCC 
AGCAGCACAA TCACATCCAG CTCATCCGTC ACCGAAACCG AACGCTTTGA TGAGTTTAAC 
CTGTCTCTTC GCTCAGACAT GGCCTCGGTC GCTAAAGCCA TGGCATCTCC AGAGTCCGGT 
CTAGAAGTCA GAGATCGAAT GTGGCTGAAG ATCACCATTC CCAATGCTTT TCTGGGTTCA 
GATGTGGTGG AGTGGTTGTA CCATCACATT GATGGCTTTC AGGACAGACG CGAAGCTCGG 
AAATATGCCT GTAACTTACT AAAGGCTGGT TTCATCAGGC ACACTGTCAA CAAGATCACC 
TTCTCTGAAC AGTGCTACTA CATATTTGGA GACTTCAGCA ACTGTGAAAA CTACATGGCC 
AACCTTTCTC TCAATGACAA TGATGGATCC AGCGGTGCTT CAGACCAGGA CACTCTGGCT 
CCTCTTCCTC TGCCAGGGGC GACACCTTGG CCTCTGCTCC ACTCGTTCAC ATACCAGTAC 
CCTCATCCTT ACTCCACCCA GCCTCCGCCA TACCATGAGC TCTCTAACTA CAGCTACGGC 
CCAGGCAGTG CAGGCAGTCA GCATAGTGAA GGGAGTCACA GTAGTGGCTC CACACGCAGC 
GATGGAGAAA AGAGAAGGGG CCCTAAAAGC GTTTCTGAGA GTACTGTGGG CGGCAGCGTG 
CGTGATGATA AATCTCCTGG CGGGGGTGGA GCTGACTCAC GTTCTGGCAG CGGCAGCGAA 
TCAGATTACT CTGTTCGCAG CACCCTGAGA CGAGATCATG GCTCAGCCAC ACCCAGCGAA 
CACAGCCGCT CGAGTCAGCG CTCGCATCAC CGCGTCCCGC CCCCTCACCT CGCCCCGTAT 
CCGCCCGGCA TCCCCATCCC ATACAACCCA ATGATGGTGA TGATGGTGCC CCAACACCCA 
CATCTGGCGC TTGGAGCACC ACATCCGCAA ACACCTACTC TGCCGCCCCA CCCTGGCTTA 
CCCCCAACCG GCATCCCCGG CGGGCCTCCA GGAGCTCCCC CGACCCGAGA CCTGGGCTCC 
GTCCCGCCTG AGCTGACTGC TTCCAGGCAG TCCTTCCACC TGGCCATGGG CAACCCCAGT 
GAGTTTTTTG TGGATGTGAT GTAA