actn3b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	actn3b
Entrez Gene ID	323320
Full Name	actinin alpha 3b
Synonyms	fb95c03,wu:fb95c03,wu:fc11d03,zgc:77243
General protein information	Preferred Names actinin alpha 3b Names actinin alpha 3b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_205544.2	NP_991107.2	actinin alpha 3b
XM_021477400.1	XP_021333075.1	actinin alpha 3b isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ACTN3	XP_005631486.1
Anopheles gambiae (African malaria mosquito)	ACTN_ANOGA	XP_321625.2
Bos taurus (cattle)	ACTN3	NP_001069625.1
Mus musculus (house mouse)	Actn3	NP_038484.1
Pan troglodytes (chimpanzee)	ACTN3	XP_003313215.1
	ACTN3	XP_001109839.1
Danio rerio (zebrafish)	actn3b	NP_991107.1
Drosophila melanogaster (fruit fly)	Actn	NP_477485.1
Rattus norvegicus (Norway rat)	Actn3	NP_596915.1
Danio rerio (zebrafish)	actn3a	NP_571597.1
Caenorhabditis elegans (roundworm)	atn-1	NP_506128.1
Xenopus tropicalis (tropical clawed frog)	actn3	NP_001135513.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Proteolysis regulates cardiomyocyte maturation and tissue integration.
Fukuda R, Gunawan F, Beisaw A, Jimenez-Amilburu V, Maischein HM, Kostin S, Kawakami K, Stainier DY
Nature communications814495(2017 Feb)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Explant culture of adult zebrafish hearts for epicardial regeneration studies.
Cao J, Poss KD
Nature protocols11(5)872-81(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following actn3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the actn3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa60497	NM_205544.2 Latest version!	Danio rerio actinin alpha 3b (actn3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa143201	XM_021477400.1 Latest version!	Danio rerio actinin alpha 3b (actn3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

actn3b ( NM_205544.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_991107.2 Danio rerio actinin alpha 3b (actn3b), mRNA.

Price & Availability↑

CloneID	ODa60497
Clone ID Related Accession (Same CDS sequence)	NM_205544.2
Accession Version	NM_205544.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	actinin alpha 3b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL808019.6. On Jan 28, 2016 this sequence version replaced NM_205544.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the reference genome strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF524840.1, BC065595.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA3505370 [ECO:0000348] ##Evidence-Data-END##

ATGACGGCTG TTGTTGAGAC GCAGATGCAG TATAACAACA CCTACATGAT GTCTACTACG 
CAAGACTACA TGATTCAGGA GGATGATTGG GACAGGGACC TGCTGCTGGA CCCTGCCTGG 
GAGAAACAGC AGAGGAAGAC GTTCACGGCG TGGTGTAATT CTCATCTGCG TAAAGCGGGA 
ACTCAGATCG AGAACATTGA AGAGGATTTC AGGAATGGCC TCAAACTCAT GCTGCTGCTC 
GAGGTCATCT CAGGTGAGAG GCTGCCCAAA CCTGACAAAG GAAAGATGCG TTTCCACAAG 
ATCGCCAACG TCAACAAGGC TCTGGATTTC ATCTGTAGCA AAGGAGTCAA ACTGGTGTCC 
ATCGGTGCTG AAGAGATTGT TGATGGTAAT GTGAAAATGA CTCTGGGGAT GATCTGGACC 
ATCATCCTGC GTTTCGCCAT CCAGGACATC TCTGTGGAGG AGACGTCTGC TAAAGAGGGT 
CTTCTGTTGT GGTGTCAGAG GAAGACTGCC CCCTACAGGA ACGTCAATGT ACAGAACTTC 
CACATCAGCT GGAAGGACGG CCTGGCTCTG TGCGCCCTCA TCCACAGACA CAGACCAGAC 
CTCATCGACT ACTCCAAACT CAGAAAGGAC GACCCCATTG GAAACCTGAA CACTGCATTT 
GAGGTGGCTG AGAAATACCT GGACATCCCC AAAATGCTGG ACGCTGAAGA CATTGTCAAC 
ACTCCTAAAC CTGACGAGAA AGCCATCATG ACCTACGTGT CCTGCTTTTA CCATGCTTTC 
GCTGGTGCTG AACAGGCTGA GACGGCTGCT AACAGGATCT GCAAGGTTCT AGCTGTGAAC 
CAGGAGAATG AACGGCTGAT GGAGGAGTAT GAGAAACTGG CCAGCGAGCT GCTGGAGTGG 
ATCCGCCGCA CCATCCCCTG GCTGGAGAAC CGCGCGGCGG AGCAGACCAT GCGCGCCATG 
CAGCAGAAGC TGGAGGATTT CCGGGACTAC AGGCGCGTTC ACAAGCCTCC GCGCGTGCAG 
GAGAAATGCC AGCTGGAGAT CAACTTCAAC ACCCTGCAGA CCAAGCTGCG CCTGAGCAAC 
CGTCCCGCCT TCATGCCCTC CGAGGGGAAG ATGGTGTCGG ACATCGCTAA CGCTTGGAAA 
GGTCTGGAGC AGGTGGAGAA GGGTTATGAG GAATGGCTGC TCACTGAGAT CCGCCGTCTG 
GAGAGACTCG ATCATTTGGC TGAGAAGTTT AAGCAGAAAT GCTCCCTGCA TGAGTCCTGG 
ACCACAGGAA AGGAGCACCT GCTGTCCCAG AAGGACTATG AGACGGCCTC TCTGATGGAG 
ATCCGGGCTC TGATGAGGAA ACACGAGGCG TTCGAGAGCG ATCTGGCCGC TCATCAGGAC 
CGCGTGGAGC AGATTGCAGC CATCGCTCAA GAGCTGAATG AATTAGACTA TCATGATGCT 
GCTACAGTGA ACGCTCGCTG TCAGGGCATC TGTGACCAGT GGGACAATCT GGGGACCCTG 
ACCCAGAAGA GGAGAGATTC TCTGGAGCGT GTAGAAAAGC TTTGGGAGAC GATTGATCAG 
CTGTACCTGG AGTTCGCCAA GAGAGCAGCT CCCTTCAACA ACTGGATGGA CGGAGCCATT 
GAGGATCTGC AGGACATGTT CATCGTGCAC AGCATCGAGG AAATCCAGAG TCTGATCACA 
GCTCACGACC AGTTTAAAGC CACTCTTCCC GAGGCCGATA AGGAGCGAAT GGCCGTGATG 
GGCATCCAGA ACGAGATCGT GAAGATCGCT CAAACCTACG GCATCAAGCT GGTCGGAGTC 
AATCCATACT CAGTGCTCAG CCCACAGGAC ATCACCAACA AATGGGAGGC TGTCAAGCAG 
CTGGTTCCCC TCAGAGACCA GATGCTGCAG GAGGAGGTTG CCAGGCAACA GGCCAACGAG 
AGGCTGAGAC GCTCGTTTGC GGCTCAGGCA AACATCATCG GACCCTGGAT TCAGACCAAG 
ATGGAGGAGA TCGGTCATGT GTCGGTGGAC ATCGCCGGCT CTCTGGAGGA ACAGATGAAC 
AACCTGAAGC AGTACGAGCA GAACATCATC AACTATAAAT CCAACATCGA CAAGCTGGAG 
GGAGACCACC AGCTCATTCA GGAGGCGCTC ATCTTCGACA ACAAACACAC CAACTACACC 
ATGGAGCACA TCCGTGTGGG CTGGGAGCAG CTGCTCACCA CCATCGCTCG CACCATCAAC 
GAGGTGGAGA ACCAGATCCT GACCCGCGAC GCAAAGGGCA TCAGCCAGGA GCAGCTCAAC 
GAGTTCAGAG CCTCCTTCAA CCACTTCGAC AGGAAGAGAA ACGGCATGAT GGACCCCGAC 
GACTTCCGCG CCTGTCTGAT CTCTATGGGT TACGATCTGG GTGAGGTGGA GTTCGCCCGC 
ATTATGACCC TGGTGGACCC CAACAACACC GGCGTGGTGA CCTTCCAGGC CTTCATCGAC 
TTCATGACCC GCGAGACTGC CGAAACCGAC ACTGCTGAAC AAGTCATGGC CTCCTTCAAG 
ATCCTGGCCT CCGACAAGCC CTACATCACC GTGGAGGAGC TCCGCCGCGA ACTGCCCCCA 
GAGCAGGCCG AGTACTGCAT CAGCCGCATG ACCAAGTACG TCGGCCCCGA AGGAGCCCTT 
GGAGCCCTGG ATTACATCTC CTTCTCCAGC GCCCTCTACG GAGAGAGCGA CTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_991107.2
CDS	174..2870
Translation	MTAVVETQMQYNNTYMMSTTQDYMIQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPKPDKGKMRFHKIANVNKALDFICSKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNVQNFHISWKDGLALCALIHRHRPDLIDYSKLRKDDPIGNLNTAFEVAEKYLDIPKMLDAEDIVNTPKPDEKAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENERLMEEYEKLASELLEWIRRTIPWLENRAAEQTMRAMQQKLEDFRDYRRVHKPPRVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDIANAWKGLEQVEKGYEEWLLTEIRRLERLDHLAEKFKQKCSLHESWTTGKEHLLSQKDYETASLMEIRALMRKHEAFESDLAAHQDRVEQIAAIAQELNELDYHDAATVNARCQGICDQWDNLGTLTQKRRDSLERVEKLWETIDQLYLEFAKRAAPFNNWMDGAIEDLQDMFIVHSIEEIQSLITAHDQFKATLPEADKERMAVMGIQNEIVKIAQTYGIKLVGVNPYSVLSPQDITNKWEAVKQLVPLRDQMLQEEVARQQANERLRRSFAAQANIIGPWIQTKMEEIGHVSVDIAGSLEEQMNNLKQYEQNIINYKSNIDKLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQLNEFRASFNHFDRKRNGMMDPDDFRACLISMGYDLGEVEFARIMTLVDPNNTGVVTFQAFIDFMTRETAETDTAEQVMASFKILASDKPYITVEELRRELPPEQAEYCISRMTKYVGPEGALGALDYISFSSALYGESDL

Target ORF information:

RefSeq Version	NM_205544.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actinin alpha 3b (actn3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205544.2

ORF Insert Sequence:

ATGACGGCTG TTGTTGAGAC GCAGATGCAG TATAACAACA CCTACATGAT GTCTACTACG 
CAAGACTACA TGATTCAGGA GGATGATTGG GACAGGGACC TGCTGCTGGA CCCTGCCTGG 
GAGAAACAGC AGAGGAAGAC GTTCACGGCG TGGTGTAATT CTCATCTGCG TAAAGCGGGA 
ACTCAGATCG AGAACATTGA AGAGGATTTC AGGAATGGCC TCAAACTCAT GCTGCTGCTC 
GAGGTCATCT CAGGTGAGAG GCTGCCCAAA CCTGACAAAG GAAAGATGCG TTTCCACAAG 
ATCGCCAACG TCAACAAGGC TCTGGATTTC ATCTGTAGCA AAGGAGTCAA ACTGGTGTCC 
ATCGGTGCTG AAGAGATTGT TGATGGTAAT GTGAAAATGA CTCTGGGGAT GATCTGGACC 
ATCATCCTGC GTTTCGCCAT CCAGGACATC TCTGTGGAGG AGACGTCTGC TAAAGAGGGT 
CTTCTGTTGT GGTGTCAGAG GAAGACTGCC CCCTACAGGA ACGTCAATGT ACAGAACTTC 
CACATCAGCT GGAAGGACGG CCTGGCTCTG TGCGCCCTCA TCCACAGACA CAGACCAGAC 
CTCATCGACT ACTCCAAACT CAGAAAGGAC GACCCCATTG GAAACCTGAA CACTGCATTT 
GAGGTGGCTG AGAAATACCT GGACATCCCC AAAATGCTGG ACGCTGAAGA CATTGTCAAC 
ACTCCTAAAC CTGACGAGAA AGCCATCATG ACCTACGTGT CCTGCTTTTA CCATGCTTTC 
GCTGGTGCTG AACAGGCTGA GACGGCTGCT AACAGGATCT GCAAGGTTCT AGCTGTGAAC 
CAGGAGAATG AACGGCTGAT GGAGGAGTAT GAGAAACTGG CCAGCGAGCT GCTGGAGTGG 
ATCCGCCGCA CCATCCCCTG GCTGGAGAAC CGCGCGGCGG AGCAGACCAT GCGCGCCATG 
CAGCAGAAGC TGGAGGATTT CCGGGACTAC AGGCGCGTTC ACAAGCCTCC GCGCGTGCAG 
GAGAAATGCC AGCTGGAGAT CAACTTCAAC ACCCTGCAGA CCAAGCTGCG CCTGAGCAAC 
CGTCCCGCCT TCATGCCCTC CGAGGGGAAG ATGGTGTCGG ACATCGCTAA CGCTTGGAAA 
GGTCTGGAGC AGGTGGAGAA GGGTTATGAG GAATGGCTGC TCACTGAGAT CCGCCGTCTG 
GAGAGACTCG ATCATTTGGC TGAGAAGTTT AAGCAGAAAT GCTCCCTGCA TGAGTCCTGG 
ACCACAGGAA AGGAGCACCT GCTGTCCCAG AAGGACTATG AGACGGCCTC TCTGATGGAG 
ATCCGGGCTC TGATGAGGAA ACACGAGGCG TTCGAGAGCG ATCTGGCCGC TCATCAGGAC 
CGCGTGGAGC AGATTGCAGC CATCGCTCAA GAGCTGAATG AATTAGACTA TCATGATGCT 
GCTACAGTGA ACGCTCGCTG TCAGGGCATC TGTGACCAGT GGGACAATCT GGGGACCCTG 
ACCCAGAAGA GGAGAGATTC TCTGGAGCGT GTAGAAAAGC TTTGGGAGAC GATTGATCAG 
CTGTACCTGG AGTTCGCCAA GAGAGCAGCT CCCTTCAACA ACTGGATGGA CGGAGCCATT 
GAGGATCTGC AGGACATGTT CATCGTGCAC AGCATCGAGG AAATCCAGAG TCTGATCACA 
GCTCACGACC AGTTTAAAGC CACTCTTCCC GAGGCCGATA AGGAGCGAAT GGCCGTGATG 
GGCATCCAGA ACGAGATCGT GAAGATCGCT CAAACCTACG GCATCAAGCT GGTCGGAGTC 
AATCCATACT CAGTGCTCAG CCCACAGGAC ATCACCAACA AATGGGAGGC TGTCAAGCAG 
CTGGTTCCCC TCAGAGACCA GATGCTGCAG GAGGAGGTTG CCAGGCAACA GGCCAACGAG 
AGGCTGAGAC GCTCGTTTGC GGCTCAGGCA AACATCATCG GACCCTGGAT TCAGACCAAG 
ATGGAGGAGA TCGGTCATGT GTCGGTGGAC ATCGCCGGCT CTCTGGAGGA ACAGATGAAC 
AACCTGAAGC AGTACGAGCA GAACATCATC AACTATAAAT CCAACATCGA CAAGCTGGAG 
GGAGACCACC AGCTCATTCA GGAGGCGCTC ATCTTCGACA ACAAACACAC CAACTACACC 
ATGGAGCACA TCCGTGTGGG CTGGGAGCAG CTGCTCACCA CCATCGCTCG CACCATCAAC 
GAGGTGGAGA ACCAGATCCT GACCCGCGAC GCAAAGGGCA TCAGCCAGGA GCAGCTCAAC 
GAGTTCAGAG CCTCCTTCAA CCACTTCGAC AGGAAGAGAA ACGGCATGAT GGACCCCGAC 
GACTTCCGCG CCTGTCTGAT CTCTATGGGT TACGATCTGG GTGAGGTGGA GTTCGCCCGC 
ATTATGACCC TGGTGGACCC CAACAACACC GGCGTGGTGA CCTTCCAGGC CTTCATCGAC 
TTCATGACCC GCGAGACTGC CGAAACCGAC ACTGCTGAAC AAGTCATGGC CTCCTTCAAG 
ATCCTGGCCT CCGACAAGCC CTACATCACC GTGGAGGAGC TCCGCCGCGA ACTGCCCCCA 
GAGCAGGCCG AGTACTGCAT CAGCCGCATG ACCAAGTACG TCGGCCCCGA AGGAGCCCTT 
GGAGCCCTGG ATTACATCTC CTTCTCCAGC GCCCTCTACG GAGAGAGCGA CTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

actn3b ( XM_021477400.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333075.1 Danio rerio actinin alpha 3b (actn3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa143201
Clone ID Related Accession (Same CDS sequence)	XM_021477400.1
Accession Version	XM_021477400.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	actinin alpha 3b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCTG TTGTCGAGAC GCAAATGCAG TATAACAACA CCTACATGAT GTCTACTACG 
CAAGACTACA TGATTCAGGA GGATGATTGG GACAGGGACC TGCTGCTGGA CCCTGCCTGG 
GAGAAACAGC AGAGGAAGAC GTTCACGGCG TGGTGTAATT CTCATCTGCG TAAAGCGGGA 
ACTCAGATCG AGAACATTGA AGAGGATTTC AGGAATGGCC TCAAACTCAT GCTGCTGCTC 
GAGGTCATCT CAGGTGAGAG GCTGCCCAAA CCTGACAAAG GAAAGATGCG TTTCCACAAG 
ATCGCCAACG TCAACAAGGC TCTGGATTTC ATCTGTAGCA AAGGAGTCAA ACTGGTGTCC 
ATCGGTGCTG AAGAGATTGT TGATGGTAAT GTGAAAATGA CTCTGGGGAT GATCTGGACC 
ATCATCCTGC GTTTCGCCAT CCAGGACATC TCTGTGGAGG AGACGTCTGC TAAAGAGGGT 
CTTCTGTTGT GGTGTCAGAG GAAGACTGCC CCCTACAGGA ACGTCAATGT ACAGAACTTC 
CACATCAGCT GGAAGGACGG CCTGGCTCTG TGCGCCCTCA TCCACAGACA CAGACCAGAC 
CTCATCGACT ACTCCAAACT CAGAAAGGAC GACCCCATTG GAAACCTGAA CACTGCATTT 
GAGGTGGCTG AGAAATACCT GGACATCCCC AAAATGCTGG ACGCTGAAGA CATTGTCAAC 
ACTCCTAAAC CTGACGAGAA AGCCATCATG ACCTACGTGT CCTGCTTTTA CCATGCTTTC 
GCTGGTGCTG AACAGGCTGA GACGGCTGCT AACAGGATCT GCAAGGTTCT AGCTGTGAAC 
CAGGAGAATG AACGGCTGAT GGAGGAGTAT GAGAAACTGG CCAGCGAGCT GCTGGAGTGG 
ATCCGCCGCA CCATCCCCTG GCTGGAGAAC CGCGCGGCGG AGCAGACCAT GCGCGCCATG 
CAGCAGAAGC TGGAGGATTT CCGGGACTAC AGGCGCGTTC ACAAGCCTCC GCGCGTGCAG 
GAGAAATGCC AGCTGGAGAT CAACTTCAAC ACCCTGCAGA CCAAGCTGCG CCTGAGCAAC 
CGTCCCGCCT TCATGCCCTC CGAGGGGAAG ATGGTGTCGG ACATCGCTAA CGCTTGGAAA 
GGTCTGGAGC AGGTGGAGAA GGGTTATGAG GAATGGCTGC TCACTGAGAT CCGCCGTCTG 
GAGAGACTCG ATCATTTGGC TGAGAAGTTT AAGCAGAAAT GCTCCCTGCA TGAGTCCTGG 
ACCACAGGAA AGGAGCACCT GCTGTCCCAG AAGGACTATG AGACGGCCTC TCTGATGGAG 
ATCCGGGCTC TGATGAGGAA ACACGAGGCG TTCGAGAGCG ATCTGGCCGC TCATCAGGAC 
CGCGTGGAGC AGATTGCAGC CATCGCTCAA GAGCTGAATG AATTAGACTA TCATGATGCT 
GCTACAGTGA ACGCTCGCTG TCAGGGCATC TGTGACCAGT GGGACAATCT GGGGACCCTG 
ACCCAGAAGA GGAGAGATTC TCTGGAGCGT GTAGAAAAGC TTTGGGAGAC GATTGATCAG 
CTGTACCTGG AGTTCGCCAA GAGAGCAGCT CCCTTCAACA ACTGGATGGA CGGAGCCATT 
GAGGATCTGC AGGACATGTT CATCGTGCAC AGCATCGAGG AAATCCAGAG TCTGATCACA 
GCTCACGACC AGTTTAAAGC CACTCTTCCC GAGGCCGATA AGGAGCGAAT GGCCGTGATG 
GGCATCCAGA ACGAGATCGT GAAGATCGCT CAAACCTACG GCATCAAGCT GGTCGGAGTC 
AATCCATACT CAGTGCTCAG CCCACAGGAC ATCACCAACA AATGGGAGGC TGTCAAGCAG 
CTGGTTCCCC TCAGAGACCA GATGCTGCAG GAGGAGGTTG CCAGGCAACA GGCCAACGAG 
AGGCTGAGAC GCTCGTTTGC GGCTCAGGCA AACATCATCG GACCCTGGAT TCAGACCAAG 
ATGGAGGAGA TCGGTCATGT GTCGGTGGAC ATCGCCGGCT CTCTGGAGGA ACAGATGAAC 
AACCTGAAGC AGTACGAGCA GAACATCATC AACTATAAAT CCAACATCGA CAAGCTGGAG 
GGAGACCACC AGCTCATTCA GGAGGCGCTC ATCTTCGACA ACAAACACAC CAACTACACC 
ATGGAGCACA TCCGTGTGGG CTGGGAGCAG CTGCTCACCA CCATCGCTCG CACCATCAAC 
GAGGTGGAGA ACCAGATCCT GACCCGCGAC GCAAAGGGCA TCAGCCAGGA GCAGCTCAAC 
GAGTTCAGAG CCTCCTTCAA CCACTTCGAC AGGAAGAGAA ACGGCATGAT GGACCCCGAC 
GACTTCCGCG CCTGTCTGAT CTCTATGGGT TACGATCTGG GTGAGGTGGA GTTCGCCCGC 
ATTATGACCC TGGTGGACCC CAACAACACC GGCGTGGTGA CCTTCCAGGC CTTCATCGAC 
TTCATGACCC GCGAGACTGC CGAAACCGAC ACTGCTGAAC AAGTCATGGC CTCCTTCAAG 
ATCCTGGCCT CCGACAAGCC CTACATCACC GTGGAGGAGC TCCGCCGCGA ACTGCCCCCA 
GAGCAGGCCG AGTACTGCAT CAGCCGCATG ACCAAGTACG TCGGCCCCGA AGGAGCCCTT 
GGAGCCCTGG ATTACATCTC CTTCTCCAGC GCCCTCTACG GAGAGAGCGA CTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333075.1
CDS	177..2873
Translation	MTAVVETQMQYNNTYMMSTTQDYMIQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPKPDKGKMRFHKIANVNKALDFICSKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNVQNFHISWKDGLALCALIHRHRPDLIDYSKLRKDDPIGNLNTAFEVAEKYLDIPKMLDAEDIVNTPKPDEKAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENERLMEEYEKLASELLEWIRRTIPWLENRAAEQTMRAMQQKLEDFRDYRRVHKPPRVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDIANAWKGLEQVEKGYEEWLLTEIRRLERLDHLAEKFKQKCSLHESWTTGKEHLLSQKDYETASLMEIRALMRKHEAFESDLAAHQDRVEQIAAIAQELNELDYHDAATVNARCQGICDQWDNLGTLTQKRRDSLERVEKLWETIDQLYLEFAKRAAPFNNWMDGAIEDLQDMFIVHSIEEIQSLITAHDQFKATLPEADKERMAVMGIQNEIVKIAQTYGIKLVGVNPYSVLSPQDITNKWEAVKQLVPLRDQMLQEEVARQQANERLRRSFAAQANIIGPWIQTKMEEIGHVSVDIAGSLEEQMNNLKQYEQNIINYKSNIDKLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQLNEFRASFNHFDRKRNGMMDPDDFRACLISMGYDLGEVEFARIMTLVDPNNTGVVTFQAFIDFMTRETAETDTAEQVMASFKILASDKPYITVEELRRELPPEQAEYCISRMTKYVGPEGALGALDYISFSSALYGESDL

Target ORF information:

RefSeq Version	XM_021477400.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actinin alpha 3b (actn3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477400.1

ORF Insert Sequence:

ATGACGGCTG TTGTCGAGAC GCAAATGCAG TATAACAACA CCTACATGAT GTCTACTACG 
CAAGACTACA TGATTCAGGA GGATGATTGG GACAGGGACC TGCTGCTGGA CCCTGCCTGG 
GAGAAACAGC AGAGGAAGAC GTTCACGGCG TGGTGTAATT CTCATCTGCG TAAAGCGGGA 
ACTCAGATCG AGAACATTGA AGAGGATTTC AGGAATGGCC TCAAACTCAT GCTGCTGCTC 
GAGGTCATCT CAGGTGAGAG GCTGCCCAAA CCTGACAAAG GAAAGATGCG TTTCCACAAG 
ATCGCCAACG TCAACAAGGC TCTGGATTTC ATCTGTAGCA AAGGAGTCAA ACTGGTGTCC 
ATCGGTGCTG AAGAGATTGT TGATGGTAAT GTGAAAATGA CTCTGGGGAT GATCTGGACC 
ATCATCCTGC GTTTCGCCAT CCAGGACATC TCTGTGGAGG AGACGTCTGC TAAAGAGGGT 
CTTCTGTTGT GGTGTCAGAG GAAGACTGCC CCCTACAGGA ACGTCAATGT ACAGAACTTC 
CACATCAGCT GGAAGGACGG CCTGGCTCTG TGCGCCCTCA TCCACAGACA CAGACCAGAC 
CTCATCGACT ACTCCAAACT CAGAAAGGAC GACCCCATTG GAAACCTGAA CACTGCATTT 
GAGGTGGCTG AGAAATACCT GGACATCCCC AAAATGCTGG ACGCTGAAGA CATTGTCAAC 
ACTCCTAAAC CTGACGAGAA AGCCATCATG ACCTACGTGT CCTGCTTTTA CCATGCTTTC 
GCTGGTGCTG AACAGGCTGA GACGGCTGCT AACAGGATCT GCAAGGTTCT AGCTGTGAAC 
CAGGAGAATG AACGGCTGAT GGAGGAGTAT GAGAAACTGG CCAGCGAGCT GCTGGAGTGG 
ATCCGCCGCA CCATCCCCTG GCTGGAGAAC CGCGCGGCGG AGCAGACCAT GCGCGCCATG 
CAGCAGAAGC TGGAGGATTT CCGGGACTAC AGGCGCGTTC ACAAGCCTCC GCGCGTGCAG 
GAGAAATGCC AGCTGGAGAT CAACTTCAAC ACCCTGCAGA CCAAGCTGCG CCTGAGCAAC 
CGTCCCGCCT TCATGCCCTC CGAGGGGAAG ATGGTGTCGG ACATCGCTAA CGCTTGGAAA 
GGTCTGGAGC AGGTGGAGAA GGGTTATGAG GAATGGCTGC TCACTGAGAT CCGCCGTCTG 
GAGAGACTCG ATCATTTGGC TGAGAAGTTT AAGCAGAAAT GCTCCCTGCA TGAGTCCTGG 
ACCACAGGAA AGGAGCACCT GCTGTCCCAG AAGGACTATG AGACGGCCTC TCTGATGGAG 
ATCCGGGCTC TGATGAGGAA ACACGAGGCG TTCGAGAGCG ATCTGGCCGC TCATCAGGAC 
CGCGTGGAGC AGATTGCAGC CATCGCTCAA GAGCTGAATG AATTAGACTA TCATGATGCT 
GCTACAGTGA ACGCTCGCTG TCAGGGCATC TGTGACCAGT GGGACAATCT GGGGACCCTG 
ACCCAGAAGA GGAGAGATTC TCTGGAGCGT GTAGAAAAGC TTTGGGAGAC GATTGATCAG 
CTGTACCTGG AGTTCGCCAA GAGAGCAGCT CCCTTCAACA ACTGGATGGA CGGAGCCATT 
GAGGATCTGC AGGACATGTT CATCGTGCAC AGCATCGAGG AAATCCAGAG TCTGATCACA 
GCTCACGACC AGTTTAAAGC CACTCTTCCC GAGGCCGATA AGGAGCGAAT GGCCGTGATG 
GGCATCCAGA ACGAGATCGT GAAGATCGCT CAAACCTACG GCATCAAGCT GGTCGGAGTC 
AATCCATACT CAGTGCTCAG CCCACAGGAC ATCACCAACA AATGGGAGGC TGTCAAGCAG 
CTGGTTCCCC TCAGAGACCA GATGCTGCAG GAGGAGGTTG CCAGGCAACA GGCCAACGAG 
AGGCTGAGAC GCTCGTTTGC GGCTCAGGCA AACATCATCG GACCCTGGAT TCAGACCAAG 
ATGGAGGAGA TCGGTCATGT GTCGGTGGAC ATCGCCGGCT CTCTGGAGGA ACAGATGAAC 
AACCTGAAGC AGTACGAGCA GAACATCATC AACTATAAAT CCAACATCGA CAAGCTGGAG 
GGAGACCACC AGCTCATTCA GGAGGCGCTC ATCTTCGACA ACAAACACAC CAACTACACC 
ATGGAGCACA TCCGTGTGGG CTGGGAGCAG CTGCTCACCA CCATCGCTCG CACCATCAAC 
GAGGTGGAGA ACCAGATCCT GACCCGCGAC GCAAAGGGCA TCAGCCAGGA GCAGCTCAAC 
GAGTTCAGAG CCTCCTTCAA CCACTTCGAC AGGAAGAGAA ACGGCATGAT GGACCCCGAC 
GACTTCCGCG CCTGTCTGAT CTCTATGGGT TACGATCTGG GTGAGGTGGA GTTCGCCCGC 
ATTATGACCC TGGTGGACCC CAACAACACC GGCGTGGTGA CCTTCCAGGC CTTCATCGAC 
TTCATGACCC GCGAGACTGC CGAAACCGAC ACTGCTGAAC AAGTCATGGC CTCCTTCAAG 
ATCCTGGCCT CCGACAAGCC CTACATCACC GTGGAGGAGC TCCGCCGCGA ACTGCCCCCA 
GAGCAGGCCG AGTACTGCAT CAGCCGCATG ACCAAGTACG TCGGCCCCGA AGGAGCCCTT 
GGAGCCCTGG ATTACATCTC CTTCTCCAGC GCCCTCTACG GAGAGAGCGA CTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Proteolysis regulates cardiomyocyte maturation and tissue integration.
Nature communications814495(2017 Feb)
Fukuda R,Gunawan F,Beisaw A,Jimenez-Amilburu V,Maischein HM,Kostin S,Kawakami K,Stainier DY

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Explant culture of adult zebrafish hearts for epicardial regeneration studies.
Nature protocols11(5)872-81(2016 May)
Cao J,Poss KD