eif2s1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	eif2s1b
Entrez Gene ID	321564
Full Name	eukaryotic translation initiation factor 2, subunit 1 alpha b
Synonyms	eif2,eif2s1,fb19d01,wu:fb19d01
General protein information	Preferred Names eukaryotic translation initiation factor 2, subunit 1 alpha b Names eukaryotic translation initiation factor 2 subunit 1 eIF2 alpha subunit
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131800.2	NP_571875.1	eukaryotic translation initiation factor 2 subunit 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	EIF2S1	NP_787007.1
Danio rerio (zebrafish)	eif2s1b	NP_571875.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011190	XP_309455.4
Saccharomyces cerevisiae (baker's yeast)	SUI2	NP_012540.3
Magnaporthe oryzae (rice blast fungus)	MGG_13200	XP_003712017.1
Arabidopsis thaliana (thale cress)	EIF2 ALPHA	NP_196166.1
Canis lupus familiaris (dog)	EIF2S1	XP_005623592.1
Caenorhabditis elegans (roundworm)	Y37E3.10	NP_490930.1
Neurospora crassa	NCU08277	XP_961905.1
Mus musculus (house mouse)	Eif2s1	NP_080390.1
Rattus norvegicus (Norway rat)	Eif2s1	NP_062229.1
Drosophila melanogaster (fruit fly)	eIF-2alpha	NP_573130.1
Schizosaccharomyces pombe (fission yeast)	tif211	NP_594081.1
Arabidopsis thaliana (thale cress)	AT2G40290	NP_565927.1
Xenopus tropicalis (tropical clawed frog)	eif2s1	NP_001005630.1
Homo sapiens (human)	EIF2S1	NP_004085.1
	EIF2S1	XP_001105924.1
Gallus gallus (chicken)	EIF2S1	NP_001006477.1
Kluyveromyces lactis	KLLA0A11814g	XP_451514.1
Eremothecium gossypii	AGOS_AGR320W	NP_986986.2
Oryza sativa (rice)	Os07g0692800	NP_001060724.1
Pan troglodytes (chimpanzee)	EIF2S1	XP_510016.1
Danio rerio (zebrafish)	eif2s1a	NP_955863.1
Oryza sativa (rice)	Os03g0296400	NP_001049829.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A truncated Danio rerio PKZ isoform functionally interacts with eIF2? and inhibits protein synthesis.
Liu ZY, Jia KT, Li C, Weng SP, Guo CJ, He JG
Gene527(1)292-300(2013 Sep)

Activating transcription factor 6 plays protective and pathological roles in steatosis due to endoplasmic reticulum stress in zebrafish.
Cinaroglu A, Gao C, Imrie D, Sadler KC
Hepatology (Baltimore, Md.)54(2)495-508(2011 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following eif2s1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eif2s1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35878	NM_131800.2 Latest version!	Danio rerio eukaryotic translation initiation factor 2, subunit 1 alpha b (eif2s1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eif2s1b ( NM_131800.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571875.1 Danio rerio eukaryotic translation initiation factor 2, subunit 1 alpha b (eif2s1b), mRNA.

Price & Availability↑

CloneID	ODa35878
Clone ID Related Accession (Same CDS sequence)	NM_131800.2
Accession Version	NM_131800.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-06
Organism	Danio rerio(zebrafish)
Product	eukaryotic translation initiation factor 2 subunit 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC051785.1. On or before Jul 6, 2005 this sequence version replaced XM_682496.1, NM_131800.1. ##Evidence-Data-START## Transcript exon combination :: BC051785.1, CK029858.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCCGGGTC TGAGCTGTCG GTTTTACCAG CACCGCTTCC CCGAGGTGGA GGACGTGGTG 
ATGGTGAACG TGCGCTCGAT CGCTGAGATG GGAGCGTATG TGAGTCTGCT GGAGTACAAC 
AACATCGAGG GCATGATCCT GCTGAGCGAA CTGTCCCGCA GACGCATCCG CTCCATCAAC 
AAACTCATCC GCATCGGACG CAACGAGTGT GTGGTGGTCA TCAGGGTGGA CAAGGAGAAG 
GGTTACATTG ATCTGTCCAA GAGAAGAGTG TCTCCAGAAG AAGCCATCAA ATGCGAGGAT 
AAATTCACCA AATCTAAAAC CGTGTACAGT ATTTTGCGGC ACGTGGCTGA GGTGTTGGAG 
TACACCAAAG ACGAGCAGCT GGAGAGTTTG TTCCAGAGAA CCGCTTGGGT TTTTGATGAG 
AAATACAAGA AGCCTGGATA CGGGGCCTAC GACGTCTTTA AACAAGCTGT GTCTGATCCT 
GCCATTCTGG ATGGTTTGGA TCTGACTGAG GAAGAGAGAA ACGTGCTCAT CGACAACATC 
AACAGGCGAC TCACACCACA GGCCGTCAAA ATAAGAGCTG ACATTGAGGT GGCGTGTTAT 
GGATATGAAG GCATCGATGC AGTAAAGGAG GCTCTGAGGG CAGGACTCAA CTGCTCCACT 
GAAGCCATGC CTATCAAGAT CAACCTGATC GCGCCGCCAC GGTACGTCAT GACCACCACA 
ACACTGGAGC GAACAGAAGG CCTGTCAGTG CTCAACCAGG CCATGGCCGC AATTAAAGAG 
CGGATCGAGG AGAAGCGAGG AGTCTTCAAT GTCCAGATGG AGCCCAAGGT GGTGACGGAC 
ACAGACGAGA CGGAACTGCA GCGGCAGCTC GAGCGTCTGG AGCGAGAAAA CGCAGAAGTG 
GACGGAGACG ACGATGCAGA AGAGATGGAG GCCAAAACTG AGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571875.1
CDS	150..1097
Translation	MPGLSCRFYQHRFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDEKYKKPGYGAYDVFKQAVSDPAILDGLDLTEEERNVLIDNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTEAMPIKINLIAPPRYVMTTTTLERTEGLSVLNQAMAAIKERIEEKRGVFNVQMEPKVVTDTDETELQRQLERLERENAEVDGDDDAEEMEAKTED

Target ORF information:

RefSeq Version	NM_131800.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 2, subunit 1 alpha b (eif2s1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131800.2

ORF Insert Sequence:

ATGCCGGGTC TGAGCTGTCG GTTTTACCAG CACCGCTTCC CCGAGGTGGA GGACGTGGTG 
ATGGTGAACG TGCGCTCGAT CGCTGAGATG GGAGCGTATG TGAGTCTGCT GGAGTACAAC 
AACATCGAGG GCATGATCCT GCTGAGCGAA CTGTCCCGCA GACGCATCCG CTCCATCAAC 
AAACTCATCC GCATCGGACG CAACGAGTGT GTGGTGGTCA TCAGGGTGGA CAAGGAGAAG 
GGTTACATTG ATCTGTCCAA GAGAAGAGTG TCTCCAGAAG AAGCCATCAA ATGCGAGGAT 
AAATTCACCA AATCTAAAAC CGTGTACAGT ATTTTGCGGC ACGTGGCTGA GGTGTTGGAG 
TACACCAAAG ACGAGCAGCT GGAGAGTTTG TTCCAGAGAA CCGCTTGGGT TTTTGATGAG 
AAATACAAGA AGCCTGGATA CGGGGCCTAC GACGTCTTTA AACAAGCTGT GTCTGATCCT 
GCCATTCTGG ATGGTTTGGA TCTGACTGAG GAAGAGAGAA ACGTGCTCAT CGACAACATC 
AACAGGCGAC TCACACCACA GGCCGTCAAA ATAAGAGCTG ACATTGAGGT GGCGTGTTAT 
GGATATGAAG GCATCGATGC AGTAAAGGAG GCTCTGAGGG CAGGACTCAA CTGCTCCACT 
GAAGCCATGC CTATCAAGAT CAACCTGATC GCGCCGCCAC GGTACGTCAT GACCACCACA 
ACACTGGAGC GAACAGAAGG CCTGTCAGTG CTCAACCAGG CCATGGCCGC AATTAAAGAG 
CGGATCGAGG AGAAGCGAGG AGTCTTCAAT GTCCAGATGG AGCCCAAGGT GGTGACGGAC 
ACAGACGAGA CGGAACTGCA GCGGCAGCTC GAGCGTCTGG AGCGAGAAAA CGCAGAAGTG 
GACGGAGACG ACGATGCAGA AGAGATGGAG GCCAAAACTG AGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A truncated Danio rerio PKZ isoform functionally interacts with eIF2? and inhibits protein synthesis.
Gene527(1)292-300(2013 Sep)
Liu ZY,Jia KT,Li C,Weng SP,Guo CJ,He JG

Activating transcription factor 6 plays protective and pathological roles in steatosis due to endoplasmic reticulum stress in zebrafish.
Hepatology (Baltimore, Md.)54(2)495-508(2011 Aug)
Cinaroglu A,Gao C,Imrie D,Sadler KC