POU6F1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	POU6F1
Entrez Gene ID	30334
Full Name	POU class 6 homeobox 1
Synonyms	pou[c],pouc
General protein information	Preferred Names POU class 6 homeobox 1 Names POU domain, class 6, transcription factor 1 POU domain gene C POU domain protein C ZFPOU[C]
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005162296.3	XP_005162353.1	POU domain, class 6, transcription factor 1 isoform X1
NM_131113.1	NP_571188.1	POU domain, class 6, transcription factor 1
XM_005162296.4	XP_005162353.1	POU domain, class 6, transcription factor 1 isoform X1
XM_017353502.2	XP_017208991.1	POU domain, class 6, transcription factor 1 isoform X2
XM_017353502.1	XP_017208991.1	POU domain, class 6, transcription factor 1 isoform X2

Pathways from BioSystems

Homologies

	LOC694640	XP_001083309.1
Mus musculus (house mouse)	Pou6f1	NP_034257.1
Rattus norvegicus (Norway rat)	Pou6f1	NP_001099216.1
Xenopus tropicalis (tropical clawed frog)	pou6f1	NP_001103520.2
Canis lupus familiaris (dog)	POU6F1	XP_003433575.1
Pan troglodytes (chimpanzee)	POU6F1	XP_001148929.1
Bos taurus (cattle)	POU6F1	XP_002687314.2
Danio rerio (zebrafish)	pou6f1	NP_571188.1
Homo sapiens (human)	POU6F1	NP_002693.3

Gene Ontology
Bibliography

Related articles in PubMed

Hypocretin neuron-specific transcriptome profiling identifies the sleep modulator Kcnh4a.
Yelin-Bekerman L, Elbaz I, Diber A, Dahary D, Gibbs-Bar L, Alon S, Lerer-Goldshtein T, Appelbaum L
eLife4e08638(2015 Oct)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Time point-based integrative analyses of deep-transcriptome identify four signal pathways in blastemal regeneration of zebrafish lower jaw.
Zhang H, Wang X, Lyu K, Gao S, Wang G, Fan C, Zhang XA, Yan J
Stem cells (Dayton, Ohio)33(3)806-18(2015 Mar)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POU6F1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POU6F1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa37690	NM_131113.1 Latest version!	Danio rerio POU class 6 homeobox 1 (pou6f1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa27874	XM_005162296.4 Latest version!	Danio rerio POU class 6 homeobox 1 (pou6f1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa57331	XM_017353502.2 Latest version!	Danio rerio POU class 6 homeobox 1 (pou6f1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
ODa27874	XM_005162296.3	Danio rerio POU class 6 homeobox 1 (pou6f1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa57331	XM_017353502.1	Danio rerio POU class 6 homeobox 1 (pou6f1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POU6F1 ( NM_131113.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_571188.1 Danio rerio POU class 6 homeobox 1 (pou6f1), mRNA.

Price & Availability↑

CloneID	ODa37690
Clone ID Related Accession (Same CDS sequence)	NM_131113.1
Accession Version	NM_131113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Danio rerio(zebrafish)
Product	POU domain, class 6, transcription factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X68432.1. ##Evidence-Data-START## Transcript exon combination :: X68432.1, BC162146.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGACACTG AGGATCTCCC TGCAAACAAT GCACCTCTCA CTGTCAACGA GCAGCTTTTA 
GGTTCCTGCA CTTTGAAATT CCCAGCACAA GACGCGCAGG TCATTGTCAT GTCAGGCCAG 
GAGACCATCC GTGTGCTGGA GGTGGAGGTG GACACGGCCT TATCTTCTGC AGGGGCTGCA 
GAGTCTGGGG GTGATGAAGA AGGCTCTGGT CAGAGCCTGG AAGCAACAGA AGAGGCTCAA 
CTGGACGGTC CAGTAACCAC AAGCTCTACC ACAGCTGTGA CTGTGGAGGT TTCAGCACCT 
GTAGTGCAGA CTGTGGTCTC CAAAGCTGCC ATCAGTGTGT CTCCAGCTCA GCAGACCAGC 
GTTCCTATTA CTGTTCAGGC TTGCCCACAG GTCCTGACTC AGGATGGTCT GGCGTCTCTG 
ATGACTGGCA TGCTTGCCCA GCAGAGTTCT CTAGGTCAGC CGCTGCTCAT TCCTCTCAGT 
ATGGCTGGAT CAGTCGGGGG TCAGGGTGGT CTGGCGGTGC TCACTTTACC CACCGCCACT 
GTGGCCACTC TCCCCGGTCT CGCTGCTGCC AGTCCTGCTG GAGGCCTGCT GAAGCTGCCG 
TTTGCTGGAC TGCAAGCTGC TACTGTGTTG AACTCTGTGC AGACCCAGCT TCAGGCACCT 
GCGCAGGCCG TCTTGCAGCC TCAGATGTCT GCTTTACAGG CTATGCAGCA GACGCAAACC 
ACAGCGGCCA CCACTGCGTC TATTGTGCAG AAGGCATCAG AGCCCAGTGT CTCCGTCGCA 
ACACTGCAGA CCGCAGGACT CTCCATCAAC CCTGCTATTA TTAGTGCAGC GTCTTTAGGA 
GCTCAGCCGC AGTTCATCAG TTCTCTTACT ACGACTCCCA TCATCACCAG TGCCATGTCA 
AACGTTGCAG GTCTCACAAG TCAACTCATC ACCAACGCAC AGGGCCAGGT GATAGGAACT 
CTTCCTCTGC TGGTCAACCC CGCTTCTCTG GCTGGAGCTG CAGCGGCCTC TGCTTTACCT 
GCTCAAGGCC TGCAGGTTCA GACCGTTGCT CCACAGCTAC TCCTGAACTC GCAGGGACAG 
ATCATTGCTA CGATAGGGAA CGGTCCAACA GCTGCTATCC CATCCACCGC TTCTGTCCTC 
CCCAAAGCCA CCGTTCCCCT GACCCTCACC AAAACCACCA CACAGGGTCC TGTTGGGAAA 
GTGGCCCCAT CCAAAGTGAT CATTGCTCCC CAACCGTCGG TGGTTAAACC GGTGACCTCA 
CTCACGGCTG CCGGTGTCAT CGCCTGTGGA GAAATGCCTA CCGTCGGGCA GCTTGTCAAC 
AAGCCGTCTG CTGTGAAAGA TGAAGAGGCC ATTAATCTGG AGGAGATTCG AGAGTTCGCC 
AAGAACTTTA AGATTCGACG GCTCTCTCTG GGTTTGACAC AGACTCAAGT GGGCCAAGCT 
CTGACTGCTA CCGAGGGCCC TGCGTACAGT CAGTCAGCCA TCTGCAGGTT TGAGAAGCTG 
GACATCACTC CTAAAAGCGC GCAGAAGCTG AAGCCTGTGC TGGAGCGCTG GCTGGCCGAG 
GCAGAGCTCT GGAACCAGAA AGGTCAGCAG AACTTGATGG AGTTCGTGGG AGGAGAACCG 
TCCAAGAAAC GCAAACGGAG AACGAGCTTC ACGCCACAGG CCATCGAAGT GCTCAACACT 
TATTTCGAGA AAAACTCCCT TCCGACGGGC CAGGAGATTA CAGAGATCGC CAAGGAGCTG 
AACTACGACC GAGAGGTGGT TCGGGTCTGG TTCTGCAACC GTCGACAGAC TCTGAAAAAC 
ACCAGCAAGA TTAACGTGTT TCAATCCCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571188.1
CDS	289..2121
Translation	MDTEDLPANNAPLTVNEQLLGSCTLKFPAQDAQVIVMSGQETIRVLEVEVDTALSSAGAAESGGDEEGSGQSLEATEEAQLDGPVTTSSTTAVTVEVSAPVVQTVVSKAAISVSPAQQTSVPITVQACPQVLTQDGLASLMTGMLAQQSSLGQPLLIPLSMAGSVGGQGGLAVLTLPTATVATLPGLAAASPAGGLLKLPFAGLQAATVLNSVQTQLQAPAQAVLQPQMSALQAMQQTQTTAATTASIVQKASEPSVSVATLQTAGLSINPAIISAASLGAQPQFISSLTTTPIITSAMSNVAGLTSQLITNAQGQVIGTLPLLVNPASLAGAAAASALPAQGLQVQTVAPQLLLNSQGQIIATIGNGPTAAIPSTASVLPKATVPLTLTKTTTQGPVGKVAPSKVIIAPQPSVVKPVTSLTAAGVIACGEMPTVGQLVNKPSAVKDEEAINLEEIREFAKNFKIRRLSLGLTQTQVGQALTATEGPAYSQSAICRFEKLDITPKSAQKLKPVLERWLAEAELWNQKGQQNLMEFVGGEPSKKRKRRTSFTPQAIEVLNTYFEKNSLPTGQEITEIAKELNYDREVVRVWFCNRRQTLKNTSKINVFQSQ

Target ORF information:

RefSeq Version	NM_131113.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio POU class 6 homeobox 1 (pou6f1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131113.1

ORF Insert Sequence:

ATGGACACTG AGGATCTCCC TGCAAACAAT GCACCTCTCA CTGTCAACGA GCAGCTTTTA 
GGTTCCTGCA CTTTGAAATT CCCAGCACAA GACGCGCAGG TCATTGTCAT GTCAGGCCAG 
GAGACCATCC GTGTGCTGGA GGTGGAGGTG GACACGGCCT TATCTTCTGC AGGGGCTGCA 
GAGTCTGGGG GTGATGAAGA AGGCTCTGGT CAGAGCCTGG AAGCAACAGA AGAGGCTCAA 
CTGGACGGTC CAGTAACCAC AAGCTCTACC ACAGCTGTGA CTGTGGAGGT TTCAGCACCT 
GTAGTGCAGA CTGTGGTCTC CAAAGCTGCC ATCAGTGTGT CTCCAGCTCA GCAGACCAGC 
GTTCCTATTA CTGTTCAGGC TTGCCCACAG GTCCTGACTC AGGATGGTCT GGCGTCTCTG 
ATGACTGGCA TGCTTGCCCA GCAGAGTTCT CTAGGTCAGC CGCTGCTCAT TCCTCTCAGT 
ATGGCTGGAT CAGTCGGGGG TCAGGGTGGT CTGGCGGTGC TCACTTTACC CACCGCCACT 
GTGGCCACTC TCCCCGGTCT CGCTGCTGCC AGTCCTGCTG GAGGCCTGCT GAAGCTGCCG 
TTTGCTGGAC TGCAAGCTGC TACTGTGTTG AACTCTGTGC AGACCCAGCT TCAGGCACCT 
GCGCAGGCCG TCTTGCAGCC TCAGATGTCT GCTTTACAGG CTATGCAGCA GACGCAAACC 
ACAGCGGCCA CCACTGCGTC TATTGTGCAG AAGGCATCAG AGCCCAGTGT CTCCGTCGCA 
ACACTGCAGA CCGCAGGACT CTCCATCAAC CCTGCTATTA TTAGTGCAGC GTCTTTAGGA 
GCTCAGCCGC AGTTCATCAG TTCTCTTACT ACGACTCCCA TCATCACCAG TGCCATGTCA 
AACGTTGCAG GTCTCACAAG TCAACTCATC ACCAACGCAC AGGGCCAGGT GATAGGAACT 
CTTCCTCTGC TGGTCAACCC CGCTTCTCTG GCTGGAGCTG CAGCGGCCTC TGCTTTACCT 
GCTCAAGGCC TGCAGGTTCA GACCGTTGCT CCACAGCTAC TCCTGAACTC GCAGGGACAG 
ATCATTGCTA CGATAGGGAA CGGTCCAACA GCTGCTATCC CATCCACCGC TTCTGTCCTC 
CCCAAAGCCA CCGTTCCCCT GACCCTCACC AAAACCACCA CACAGGGTCC TGTTGGGAAA 
GTGGCCCCAT CCAAAGTGAT CATTGCTCCC CAACCGTCGG TGGTTAAACC GGTGACCTCA 
CTCACGGCTG CCGGTGTCAT CGCCTGTGGA GAAATGCCTA CCGTCGGGCA GCTTGTCAAC 
AAGCCGTCTG CTGTGAAAGA TGAAGAGGCC ATTAATCTGG AGGAGATTCG AGAGTTCGCC 
AAGAACTTTA AGATTCGACG GCTCTCTCTG GGTTTGACAC AGACTCAAGT GGGCCAAGCT 
CTGACTGCTA CCGAGGGCCC TGCGTACAGT CAGTCAGCCA TCTGCAGGTT TGAGAAGCTG 
GACATCACTC CTAAAAGCGC GCAGAAGCTG AAGCCTGTGC TGGAGCGCTG GCTGGCCGAG 
GCAGAGCTCT GGAACCAGAA AGGTCAGCAG AACTTGATGG AGTTCGTGGG AGGAGAACCG 
TCCAAGAAAC GCAAACGGAG AACGAGCTTC ACGCCACAGG CCATCGAAGT GCTCAACACT 
TATTTCGAGA AAAACTCCCT TCCGACGGGC CAGGAGATTA CAGAGATCGC CAAGGAGCTG 
AACTACGACC GAGAGGTGGT TCGGGTCTGG TTCTGCAACC GTCGACAGAC TCTGAAAAAC 
ACCAGCAAGA TTAACGTGTT TCAATCCCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POU6F1 ( XM_005162296.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005162353.1 Danio rerio POU class 6 homeobox 1 (pou6f1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27874
Clone ID Related Accession (Same CDS sequence)	XM_005162296.3 , XM_005162296.4
Accession Version	XM_005162296.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	POU domain, class 6, transcription factor 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162296.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTG AGGATCTCCC TGCAAACAAT GCACCTCTCA CTGTCAACGA GCAGGTCATT 
GTCATGTCAG GCCAGGAGAC CATCCGTGTG CTGGAGGTGG AGGTGGACAC GGCCTTATCT 
TCTGCAGGGG CTGCAGAGTC TGGGGGTGAT GAAGAAGGCT CTGGTCAGAG CCTGGAAGCA 
ACAGAAGAGG CTCAACTGGA CGGTCCAGTA ACCACAAGCT CTACCACAGC TGTGACTGTG 
GAGGTTTCAG CACCTGTAGT GCAGACTGTG GTCTCCAAAG CTGCCATCAG TGTGTCTCCA 
GCTCAGCAGA CCAGCGTTCC CATTACTGTT CAGGCTTGCC CACAGGTCCT GACTCAGGAT 
GGTCTGGCGT CTCTGATGAC TGGCATGCTT GCCCAGCAGA GTTCTCTAGG TCAGCCGCTG 
CTCATTCCTC TCAGTATGGC TGGATCAGTC GGGGGTCAGG GTGGTCTGGC GGTGCTCACT 
TTACCCACCG CCACTGTGGC CACTCTCCCC GGTCTCGCTG CTGCCAGTCC TGCTGGAGGC 
CTGCTGAAGC TGCCGTTTGC TGGACTGCAA GCTGCTACTG TGTTGAACTC TGTGCAGACC 
CAGCTTCAGG CACCTGCGCA GGCCGTCTTG CAGCCTCAGA TGTCTGCTTT ACAGGCTATG 
CAGCAGACGC AAACCACAGC GGCCACCACT GCGTCTATTG TGCAGAAGGC ATCAGAGCCC 
AGTGTCTCCG TCGCAACACT GCAGACCGCA GGACTCTCCA TCAACCCTGC TATTATTAGT 
GCAGCGTCTT TAGGAGCTCA GCCGCAGTTC ATCAGCTCTC TTACTACGAC TCCCATCATC 
ACCAGTGCCA TGTCAAACGT GGCAGGTCTC ACAAGTCAAC TCATCACCAA CGCACAGGGC 
CAGGTGATAG GAACTCTTCC TCTGCTGGTC AACCCCGCTT CTTTGGCTGG AGCTGCAGCG 
GCCTCTGCTT TACCTGCTCA AGGCCTGCAG GTTCAGACCG TTGCTCCACA GCTACTCCTG 
AACTCGCAGG GACAGATCAT TGCTACGATA GGGAACGGTC CAACAGCTGC TATCCCATCC 
ACCGCTTCTG TCCTCCCCAA AGCCACCGTT CCCCTGACCC TCACCAAAAC CACCACACAG 
GGTCCTGTTG GGAAAGTGGC CCCATCCAAA GTGATCATTG CTCCCCAACC GTCAGTGGTT 
AAACCGGTGA CCTCACTCAC GGCTGCCGGT GTCATCGCCT GTGGAGAAAT GCCTACCGTC 
GGGCAGCTTG TCAACAAGCC GTCTGCTGTG AAAGATGAAG AGGCCATTAA TCTGGAGGAG 
ATTCGAGAGT TCGCCAAGAA CTTTAAGATT CGACGGCTCT CTCTGGGTTT GACACAGACT 
CAAGTGGGCC AAGCTCTGAC TGCTACCGAG GGCCCTGCGT ACAGTCAGTC AGCCATCTGC 
AGGTTTGAGA AGCTGGACAT CACTCCTAAA AGTGCGCAGA AGCTGAAGCC TGTGCTGGAG 
CGCTGGCTGG CCGAGGCAGA GCTCTGGAAC CAGAAAGGTC AGCAGAACTT GATGGAGTTC 
GTGGGAGGAG AACCGTCCAA GAAACGCAAA CGGAGAACGA GCTTCACGCC ACAGGCCATA 
GAAGTCCTCA ACACTTACTT CGAGAAAAAC TCCCTTCCGA CGGGCCAGGA GATTACAGAG 
ATCGCCAAGG AGCTGAACTA CGACCGAGAG GTGGTTCGGG TCTGGTTCTG CAACCGTCGA 
CAGACTCTGA AAAATACCAG CAAGATTAAC GTGTTTCAAT CCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162353.1
CDS	531..2318
Translation	MDTEDLPANNAPLTVNEQVIVMSGQETIRVLEVEVDTALSSAGAAESGGDEEGSGQSLEATEEAQLDGPVTTSSTTAVTVEVSAPVVQTVVSKAAISVSPAQQTSVPITVQACPQVLTQDGLASLMTGMLAQQSSLGQPLLIPLSMAGSVGGQGGLAVLTLPTATVATLPGLAAASPAGGLLKLPFAGLQAATVLNSVQTQLQAPAQAVLQPQMSALQAMQQTQTTAATTASIVQKASEPSVSVATLQTAGLSINPAIISAASLGAQPQFISSLTTTPIITSAMSNVAGLTSQLITNAQGQVIGTLPLLVNPASLAGAAAASALPAQGLQVQTVAPQLLLNSQGQIIATIGNGPTAAIPSTASVLPKATVPLTLTKTTTQGPVGKVAPSKVIIAPQPSVVKPVTSLTAAGVIACGEMPTVGQLVNKPSAVKDEEAINLEEIREFAKNFKIRRLSLGLTQTQVGQALTATEGPAYSQSAICRFEKLDITPKSAQKLKPVLERWLAEAELWNQKGQQNLMEFVGGEPSKKRKRRTSFTPQAIEVLNTYFEKNSLPTGQEITEIAKELNYDREVVRVWFCNRRQTLKNTSKINVFQSQ

Target ORF information:

RefSeq Version	XM_005162296.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio POU class 6 homeobox 1 (pou6f1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162296.3

ORF Insert Sequence:

ATGGACACTG AGGATCTCCC TGCAAACAAT GCACCTCTCA CTGTCAACGA GCAGGTCATT 
GTCATGTCAG GCCAGGAGAC CATCCGTGTG CTGGAGGTGG AGGTGGACAC GGCCTTATCT 
TCTGCAGGGG CTGCAGAGTC TGGGGGTGAT GAAGAAGGCT CTGGTCAGAG CCTGGAAGCA 
ACAGAAGAGG CTCAACTGGA CGGTCCAGTA ACCACAAGCT CTACCACAGC TGTGACTGTG 
GAGGTTTCAG CACCTGTAGT GCAGACTGTG GTCTCCAAAG CTGCCATCAG TGTGTCTCCA 
GCTCAGCAGA CCAGCGTTCC CATTACTGTT CAGGCTTGCC CACAGGTCCT GACTCAGGAT 
GGTCTGGCGT CTCTGATGAC TGGCATGCTT GCCCAGCAGA GTTCTCTAGG TCAGCCGCTG 
CTCATTCCTC TCAGTATGGC TGGATCAGTC GGGGGTCAGG GTGGTCTGGC GGTGCTCACT 
TTACCCACCG CCACTGTGGC CACTCTCCCC GGTCTCGCTG CTGCCAGTCC TGCTGGAGGC 
CTGCTGAAGC TGCCGTTTGC TGGACTGCAA GCTGCTACTG TGTTGAACTC TGTGCAGACC 
CAGCTTCAGG CACCTGCGCA GGCCGTCTTG CAGCCTCAGA TGTCTGCTTT ACAGGCTATG 
CAGCAGACGC AAACCACAGC GGCCACCACT GCGTCTATTG TGCAGAAGGC ATCAGAGCCC 
AGTGTCTCCG TCGCAACACT GCAGACCGCA GGACTCTCCA TCAACCCTGC TATTATTAGT 
GCAGCGTCTT TAGGAGCTCA GCCGCAGTTC ATCAGCTCTC TTACTACGAC TCCCATCATC 
ACCAGTGCCA TGTCAAACGT GGCAGGTCTC ACAAGTCAAC TCATCACCAA CGCACAGGGC 
CAGGTGATAG GAACTCTTCC TCTGCTGGTC AACCCCGCTT CTTTGGCTGG AGCTGCAGCG 
GCCTCTGCTT TACCTGCTCA AGGCCTGCAG GTTCAGACCG TTGCTCCACA GCTACTCCTG 
AACTCGCAGG GACAGATCAT TGCTACGATA GGGAACGGTC CAACAGCTGC TATCCCATCC 
ACCGCTTCTG TCCTCCCCAA AGCCACCGTT CCCCTGACCC TCACCAAAAC CACCACACAG 
GGTCCTGTTG GGAAAGTGGC CCCATCCAAA GTGATCATTG CTCCCCAACC GTCAGTGGTT 
AAACCGGTGA CCTCACTCAC GGCTGCCGGT GTCATCGCCT GTGGAGAAAT GCCTACCGTC 
GGGCAGCTTG TCAACAAGCC GTCTGCTGTG AAAGATGAAG AGGCCATTAA TCTGGAGGAG 
ATTCGAGAGT TCGCCAAGAA CTTTAAGATT CGACGGCTCT CTCTGGGTTT GACACAGACT 
CAAGTGGGCC AAGCTCTGAC TGCTACCGAG GGCCCTGCGT ACAGTCAGTC AGCCATCTGC 
AGGTTTGAGA AGCTGGACAT CACTCCTAAA AGTGCGCAGA AGCTGAAGCC TGTGCTGGAG 
CGCTGGCTGG CCGAGGCAGA GCTCTGGAAC CAGAAAGGTC AGCAGAACTT GATGGAGTTC 
GTGGGAGGAG AACCGTCCAA GAAACGCAAA CGGAGAACGA GCTTCACGCC ACAGGCCATA 
GAAGTCCTCA ACACTTACTT CGAGAAAAAC TCCCTTCCGA CGGGCCAGGA GATTACAGAG 
ATCGCCAAGG AGCTGAACTA CGACCGAGAG GTGGTTCGGG TCTGGTTCTG CAACCGTCGA 
CAGACTCTGA AAAATACCAG CAAGATTAAC GTGTTTCAAT CCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POU6F1 ( XM_005162296.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005162353.1 Danio rerio POU class 6 homeobox 1 (pou6f1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27874
Clone ID Related Accession (Same CDS sequence)	XM_005162296.3 , XM_005162296.4
Accession Version	XM_005162296.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	POU domain, class 6, transcription factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162296.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTG AGGATCTCCC TGCAAACAAT GCACCTCTCA CTGTCAACGA GCAGGTCATT 
GTCATGTCAG GCCAGGAGAC CATCCGTGTG CTGGAGGTGG AGGTGGACAC GGCCTTATCT 
TCTGCAGGGG CTGCAGAGTC TGGGGGTGAT GAAGAAGGCT CTGGTCAGAG CCTGGAAGCA 
ACAGAAGAGG CTCAACTGGA CGGTCCAGTA ACCACAAGCT CTACCACAGC TGTGACTGTG 
GAGGTTTCAG CACCTGTAGT GCAGACTGTG GTCTCCAAAG CTGCCATCAG TGTGTCTCCA 
GCTCAGCAGA CCAGCGTTCC CATTACTGTT CAGGCTTGCC CACAGGTCCT GACTCAGGAT 
GGTCTGGCGT CTCTGATGAC TGGCATGCTT GCCCAGCAGA GTTCTCTAGG TCAGCCGCTG 
CTCATTCCTC TCAGTATGGC TGGATCAGTC GGGGGTCAGG GTGGTCTGGC GGTGCTCACT 
TTACCCACCG CCACTGTGGC CACTCTCCCC GGTCTCGCTG CTGCCAGTCC TGCTGGAGGC 
CTGCTGAAGC TGCCGTTTGC TGGACTGCAA GCTGCTACTG TGTTGAACTC TGTGCAGACC 
CAGCTTCAGG CACCTGCGCA GGCCGTCTTG CAGCCTCAGA TGTCTGCTTT ACAGGCTATG 
CAGCAGACGC AAACCACAGC GGCCACCACT GCGTCTATTG TGCAGAAGGC ATCAGAGCCC 
AGTGTCTCCG TCGCAACACT GCAGACCGCA GGACTCTCCA TCAACCCTGC TATTATTAGT 
GCAGCGTCTT TAGGAGCTCA GCCGCAGTTC ATCAGCTCTC TTACTACGAC TCCCATCATC 
ACCAGTGCCA TGTCAAACGT GGCAGGTCTC ACAAGTCAAC TCATCACCAA CGCACAGGGC 
CAGGTGATAG GAACTCTTCC TCTGCTGGTC AACCCCGCTT CTTTGGCTGG AGCTGCAGCG 
GCCTCTGCTT TACCTGCTCA AGGCCTGCAG GTTCAGACCG TTGCTCCACA GCTACTCCTG 
AACTCGCAGG GACAGATCAT TGCTACGATA GGGAACGGTC CAACAGCTGC TATCCCATCC 
ACCGCTTCTG TCCTCCCCAA AGCCACCGTT CCCCTGACCC TCACCAAAAC CACCACACAG 
GGTCCTGTTG GGAAAGTGGC CCCATCCAAA GTGATCATTG CTCCCCAACC GTCAGTGGTT 
AAACCGGTGA CCTCACTCAC GGCTGCCGGT GTCATCGCCT GTGGAGAAAT GCCTACCGTC 
GGGCAGCTTG TCAACAAGCC GTCTGCTGTG AAAGATGAAG AGGCCATTAA TCTGGAGGAG 
ATTCGAGAGT TCGCCAAGAA CTTTAAGATT CGACGGCTCT CTCTGGGTTT GACACAGACT 
CAAGTGGGCC AAGCTCTGAC TGCTACCGAG GGCCCTGCGT ACAGTCAGTC AGCCATCTGC 
AGGTTTGAGA AGCTGGACAT CACTCCTAAA AGTGCGCAGA AGCTGAAGCC TGTGCTGGAG 
CGCTGGCTGG CCGAGGCAGA GCTCTGGAAC CAGAAAGGTC AGCAGAACTT GATGGAGTTC 
GTGGGAGGAG AACCGTCCAA GAAACGCAAA CGGAGAACGA GCTTCACGCC ACAGGCCATA 
GAAGTCCTCA ACACTTACTT CGAGAAAAAC TCCCTTCCGA CGGGCCAGGA GATTACAGAG 
ATCGCCAAGG AGCTGAACTA CGACCGAGAG GTGGTTCGGG TCTGGTTCTG CAACCGTCGA 
CAGACTCTGA AAAATACCAG CAAGATTAAC GTGTTTCAAT CCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162353.1
CDS	460..2247
Translation	MDTEDLPANNAPLTVNEQVIVMSGQETIRVLEVEVDTALSSAGAAESGGDEEGSGQSLEATEEAQLDGPVTTSSTTAVTVEVSAPVVQTVVSKAAISVSPAQQTSVPITVQACPQVLTQDGLASLMTGMLAQQSSLGQPLLIPLSMAGSVGGQGGLAVLTLPTATVATLPGLAAASPAGGLLKLPFAGLQAATVLNSVQTQLQAPAQAVLQPQMSALQAMQQTQTTAATTASIVQKASEPSVSVATLQTAGLSINPAIISAASLGAQPQFISSLTTTPIITSAMSNVAGLTSQLITNAQGQVIGTLPLLVNPASLAGAAAASALPAQGLQVQTVAPQLLLNSQGQIIATIGNGPTAAIPSTASVLPKATVPLTLTKTTTQGPVGKVAPSKVIIAPQPSVVKPVTSLTAAGVIACGEMPTVGQLVNKPSAVKDEEAINLEEIREFAKNFKIRRLSLGLTQTQVGQALTATEGPAYSQSAICRFEKLDITPKSAQKLKPVLERWLAEAELWNQKGQQNLMEFVGGEPSKKRKRRTSFTPQAIEVLNTYFEKNSLPTGQEITEIAKELNYDREVVRVWFCNRRQTLKNTSKINVFQSQ

Target ORF information:

RefSeq Version	XM_005162296.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio POU class 6 homeobox 1 (pou6f1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162296.4

ORF Insert Sequence:

ATGGACACTG AGGATCTCCC TGCAAACAAT GCACCTCTCA CTGTCAACGA GCAGGTCATT 
GTCATGTCAG GCCAGGAGAC CATCCGTGTG CTGGAGGTGG AGGTGGACAC GGCCTTATCT 
TCTGCAGGGG CTGCAGAGTC TGGGGGTGAT GAAGAAGGCT CTGGTCAGAG CCTGGAAGCA 
ACAGAAGAGG CTCAACTGGA CGGTCCAGTA ACCACAAGCT CTACCACAGC TGTGACTGTG 
GAGGTTTCAG CACCTGTAGT GCAGACTGTG GTCTCCAAAG CTGCCATCAG TGTGTCTCCA 
GCTCAGCAGA CCAGCGTTCC CATTACTGTT CAGGCTTGCC CACAGGTCCT GACTCAGGAT 
GGTCTGGCGT CTCTGATGAC TGGCATGCTT GCCCAGCAGA GTTCTCTAGG TCAGCCGCTG 
CTCATTCCTC TCAGTATGGC TGGATCAGTC GGGGGTCAGG GTGGTCTGGC GGTGCTCACT 
TTACCCACCG CCACTGTGGC CACTCTCCCC GGTCTCGCTG CTGCCAGTCC TGCTGGAGGC 
CTGCTGAAGC TGCCGTTTGC TGGACTGCAA GCTGCTACTG TGTTGAACTC TGTGCAGACC 
CAGCTTCAGG CACCTGCGCA GGCCGTCTTG CAGCCTCAGA TGTCTGCTTT ACAGGCTATG 
CAGCAGACGC AAACCACAGC GGCCACCACT GCGTCTATTG TGCAGAAGGC ATCAGAGCCC 
AGTGTCTCCG TCGCAACACT GCAGACCGCA GGACTCTCCA TCAACCCTGC TATTATTAGT 
GCAGCGTCTT TAGGAGCTCA GCCGCAGTTC ATCAGCTCTC TTACTACGAC TCCCATCATC 
ACCAGTGCCA TGTCAAACGT GGCAGGTCTC ACAAGTCAAC TCATCACCAA CGCACAGGGC 
CAGGTGATAG GAACTCTTCC TCTGCTGGTC AACCCCGCTT CTTTGGCTGG AGCTGCAGCG 
GCCTCTGCTT TACCTGCTCA AGGCCTGCAG GTTCAGACCG TTGCTCCACA GCTACTCCTG 
AACTCGCAGG GACAGATCAT TGCTACGATA GGGAACGGTC CAACAGCTGC TATCCCATCC 
ACCGCTTCTG TCCTCCCCAA AGCCACCGTT CCCCTGACCC TCACCAAAAC CACCACACAG 
GGTCCTGTTG GGAAAGTGGC CCCATCCAAA GTGATCATTG CTCCCCAACC GTCAGTGGTT 
AAACCGGTGA CCTCACTCAC GGCTGCCGGT GTCATCGCCT GTGGAGAAAT GCCTACCGTC 
GGGCAGCTTG TCAACAAGCC GTCTGCTGTG AAAGATGAAG AGGCCATTAA TCTGGAGGAG 
ATTCGAGAGT TCGCCAAGAA CTTTAAGATT CGACGGCTCT CTCTGGGTTT GACACAGACT 
CAAGTGGGCC AAGCTCTGAC TGCTACCGAG GGCCCTGCGT ACAGTCAGTC AGCCATCTGC 
AGGTTTGAGA AGCTGGACAT CACTCCTAAA AGTGCGCAGA AGCTGAAGCC TGTGCTGGAG 
CGCTGGCTGG CCGAGGCAGA GCTCTGGAAC CAGAAAGGTC AGCAGAACTT GATGGAGTTC 
GTGGGAGGAG AACCGTCCAA GAAACGCAAA CGGAGAACGA GCTTCACGCC ACAGGCCATA 
GAAGTCCTCA ACACTTACTT CGAGAAAAAC TCCCTTCCGA CGGGCCAGGA GATTACAGAG 
ATCGCCAAGG AGCTGAACTA CGACCGAGAG GTGGTTCGGG TCTGGTTCTG CAACCGTCGA 
CAGACTCTGA AAAATACCAG CAAGATTAAC GTGTTTCAAT CCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POU6F1 ( XM_017353502.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208991.1 Danio rerio POU class 6 homeobox 1 (pou6f1), transcript variant X2, mRNA.

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CloneID	ODa57331
Clone ID Related Accession (Same CDS sequence)	XM_017353502.2 , XM_017353502.1
Accession Version	XM_017353502.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	POU domain, class 6, transcription factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353502.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGCC AGGAGACCAT CCGTGTGCTG GAGGTGGAGG TGGACACGGC CTTATCTTCT 
GCAGGGGCTG CAGAGTCTGG GGGTGATGAA GAAGGCTCTG GTCAGAGCCT GGAAGCAACA 
GAAGAGGCTC AACTGGACGG TCCAGTAACC ACAAGCTCTA CCACAGCTGT GACTGTGGAG 
GTTTCAGCAC CTGTAGTGCA GACTGTGGTC TCCAAAGCTG CCATCAGTGT GTCTCCAGCT 
CAGCAGACCA GCGTTCCCAT TACTGTTCAG GCTTGCCCAC AGGTCCTGAC TCAGGATGGT 
CTGGCGTCTC TGATGACTGG CATGCTTGCC CAGCAGAGTT CTCTAGGTCA GCCGCTGCTC 
ATTCCTCTCA GTATGGCTGG ATCAGTCGGG GGTCAGGGTG GTCTGGCGGT GCTCACTTTA 
CCCACCGCCA CTGTGGCCAC TCTCCCCGGT CTCGCTGCTG CCAGTCCTGC TGGAGGCCTG 
CTGAAGCTGC CGTTTGCTGG ACTGCAAGCT GCTACTGTGT TGAACTCTGT GCAGACCCAG 
CTTCAGGCAC CTGCGCAGGC CGTCTTGCAG CCTCAGATGT CTGCTTTACA GGCTATGCAG 
CAGACGCAAA CCACAGCGGC CACCACTGCG TCTATTGTGC AGAAGGCATC AGAGCCCAGT 
GTCTCCGTCG CAACACTGCA GACCGCAGGA CTCTCCATCA ACCCTGCTAT TATTAGTGCA 
GCGTCTTTAG GAGCTCAGCC GCAGTTCATC AGCTCTCTTA CTACGACTCC CATCATCACC 
AGTGCCATGT CAAACGTGGC AGGTCTCACA AGTCAACTCA TCACCAACGC ACAGGGCCAG 
GTGATAGGAA CTCTTCCTCT GCTGGTCAAC CCCGCTTCTT TGGCTGGAGC TGCAGCGGCC 
TCTGCTTTAC CTGCTCAAGG CCTGCAGGTT CAGACCGTTG CTCCACAGCT ACTCCTGAAC 
TCGCAGGGAC AGATCATTGC TACGATAGGG AACGGTCCAA CAGCTGCTAT CCCATCCACC 
GCTTCTGTCC TCCCCAAAGC CACCGTTCCC CTGACCCTCA CCAAAACCAC CACACAGGGT 
CCTGTTGGGA AAGTGGCCCC ATCCAAAGTG ATCATTGCTC CCCAACCGTC AGTGGTTAAA 
CCGGTGACCT CACTCACGGC TGCCGGTGTC ATCGCCTGTG GAGAAATGCC TACCGTCGGG 
CAGCTTGTCA ACAAGCCGTC TGCTGTGAAA GATGAAGAGG CCATTAATCT GGAGGAGATT 
CGAGAGTTCG CCAAGAACTT TAAGATTCGA CGGCTCTCTC TGGGTTTGAC ACAGACTCAA 
GTGGGCCAAG CTCTGACTGC TACCGAGGGC CCTGCGTACA GTCAGTCAGC CATCTGCAGG 
TTTGAGAAGC TGGACATCAC TCCTAAAAGT GCGCAGAAGC TGAAGCCTGT GCTGGAGCGC 
TGGCTGGCCG AGGCAGAGCT CTGGAACCAG AAAGGTCAGC AGAACTTGAT GGAGTTCGTG 
GGAGGAGAAC CGTCCAAGAA ACGCAAACGG AGAACGAGCT TCACGCCACA GGCCATAGAA 
GTCCTCAACA CTTACTTCGA GAAAAACTCC CTTCCGACGG GCCAGGAGAT TACAGAGATC 
GCCAAGGAGC TGAACTACGA CCGAGAGGTG GTTCGGGTCT GGTTCTGCAA CCGTCGACAG 
ACTCTGAAAA ATACCAGCAA GATTAACGTG TTTCAATCCC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208991.1
CDS	603..2327
Translation	MSGQETIRVLEVEVDTALSSAGAAESGGDEEGSGQSLEATEEAQLDGPVTTSSTTAVTVEVSAPVVQTVVSKAAISVSPAQQTSVPITVQACPQVLTQDGLASLMTGMLAQQSSLGQPLLIPLSMAGSVGGQGGLAVLTLPTATVATLPGLAAASPAGGLLKLPFAGLQAATVLNSVQTQLQAPAQAVLQPQMSALQAMQQTQTTAATTASIVQKASEPSVSVATLQTAGLSINPAIISAASLGAQPQFISSLTTTPIITSAMSNVAGLTSQLITNAQGQVIGTLPLLVNPASLAGAAAASALPAQGLQVQTVAPQLLLNSQGQIIATIGNGPTAAIPSTASVLPKATVPLTLTKTTTQGPVGKVAPSKVIIAPQPSVVKPVTSLTAAGVIACGEMPTVGQLVNKPSAVKDEEAINLEEIREFAKNFKIRRLSLGLTQTQVGQALTATEGPAYSQSAICRFEKLDITPKSAQKLKPVLERWLAEAELWNQKGQQNLMEFVGGEPSKKRKRRTSFTPQAIEVLNTYFEKNSLPTGQEITEIAKELNYDREVVRVWFCNRRQTLKNTSKINVFQSQ

Target ORF information:

RefSeq Version	XM_017353502.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio POU class 6 homeobox 1 (pou6f1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353502.2

ORF Insert Sequence:

ATGTCAGGCC AGGAGACCAT CCGTGTGCTG GAGGTGGAGG TGGACACGGC CTTATCTTCT 
GCAGGGGCTG CAGAGTCTGG GGGTGATGAA GAAGGCTCTG GTCAGAGCCT GGAAGCAACA 
GAAGAGGCTC AACTGGACGG TCCAGTAACC ACAAGCTCTA CCACAGCTGT GACTGTGGAG 
GTTTCAGCAC CTGTAGTGCA GACTGTGGTC TCCAAAGCTG CCATCAGTGT GTCTCCAGCT 
CAGCAGACCA GCGTTCCCAT TACTGTTCAG GCTTGCCCAC AGGTCCTGAC TCAGGATGGT 
CTGGCGTCTC TGATGACTGG CATGCTTGCC CAGCAGAGTT CTCTAGGTCA GCCGCTGCTC 
ATTCCTCTCA GTATGGCTGG ATCAGTCGGG GGTCAGGGTG GTCTGGCGGT GCTCACTTTA 
CCCACCGCCA CTGTGGCCAC TCTCCCCGGT CTCGCTGCTG CCAGTCCTGC TGGAGGCCTG 
CTGAAGCTGC CGTTTGCTGG ACTGCAAGCT GCTACTGTGT TGAACTCTGT GCAGACCCAG 
CTTCAGGCAC CTGCGCAGGC CGTCTTGCAG CCTCAGATGT CTGCTTTACA GGCTATGCAG 
CAGACGCAAA CCACAGCGGC CACCACTGCG TCTATTGTGC AGAAGGCATC AGAGCCCAGT 
GTCTCCGTCG CAACACTGCA GACCGCAGGA CTCTCCATCA ACCCTGCTAT TATTAGTGCA 
GCGTCTTTAG GAGCTCAGCC GCAGTTCATC AGCTCTCTTA CTACGACTCC CATCATCACC 
AGTGCCATGT CAAACGTGGC AGGTCTCACA AGTCAACTCA TCACCAACGC ACAGGGCCAG 
GTGATAGGAA CTCTTCCTCT GCTGGTCAAC CCCGCTTCTT TGGCTGGAGC TGCAGCGGCC 
TCTGCTTTAC CTGCTCAAGG CCTGCAGGTT CAGACCGTTG CTCCACAGCT ACTCCTGAAC 
TCGCAGGGAC AGATCATTGC TACGATAGGG AACGGTCCAA CAGCTGCTAT CCCATCCACC 
GCTTCTGTCC TCCCCAAAGC CACCGTTCCC CTGACCCTCA CCAAAACCAC CACACAGGGT 
CCTGTTGGGA AAGTGGCCCC ATCCAAAGTG ATCATTGCTC CCCAACCGTC AGTGGTTAAA 
CCGGTGACCT CACTCACGGC TGCCGGTGTC ATCGCCTGTG GAGAAATGCC TACCGTCGGG 
CAGCTTGTCA ACAAGCCGTC TGCTGTGAAA GATGAAGAGG CCATTAATCT GGAGGAGATT 
CGAGAGTTCG CCAAGAACTT TAAGATTCGA CGGCTCTCTC TGGGTTTGAC ACAGACTCAA 
GTGGGCCAAG CTCTGACTGC TACCGAGGGC CCTGCGTACA GTCAGTCAGC CATCTGCAGG 
TTTGAGAAGC TGGACATCAC TCCTAAAAGT GCGCAGAAGC TGAAGCCTGT GCTGGAGCGC 
TGGCTGGCCG AGGCAGAGCT CTGGAACCAG AAAGGTCAGC AGAACTTGAT GGAGTTCGTG 
GGAGGAGAAC CGTCCAAGAA ACGCAAACGG AGAACGAGCT TCACGCCACA GGCCATAGAA 
GTCCTCAACA CTTACTTCGA GAAAAACTCC CTTCCGACGG GCCAGGAGAT TACAGAGATC 
GCCAAGGAGC TGAACTACGA CCGAGAGGTG GTTCGGGTCT GGTTCTGCAA CCGTCGACAG 
ACTCTGAAAA ATACCAGCAA GATTAACGTG TTTCAATCCC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POU6F1 ( XM_017353502.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208991.1 Danio rerio POU class 6 homeobox 1 (pou6f1), transcript variant X2, mRNA.

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CloneID	ODa57331
Clone ID Related Accession (Same CDS sequence)	XM_017353502.2 , XM_017353502.1
Accession Version	XM_017353502.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	POU domain, class 6, transcription factor 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGCC AGGAGACCAT CCGTGTGCTG GAGGTGGAGG TGGACACGGC CTTATCTTCT 
GCAGGGGCTG CAGAGTCTGG GGGTGATGAA GAAGGCTCTG GTCAGAGCCT GGAAGCAACA 
GAAGAGGCTC AACTGGACGG TCCAGTAACC ACAAGCTCTA CCACAGCTGT GACTGTGGAG 
GTTTCAGCAC CTGTAGTGCA GACTGTGGTC TCCAAAGCTG CCATCAGTGT GTCTCCAGCT 
CAGCAGACCA GCGTTCCCAT TACTGTTCAG GCTTGCCCAC AGGTCCTGAC TCAGGATGGT 
CTGGCGTCTC TGATGACTGG CATGCTTGCC CAGCAGAGTT CTCTAGGTCA GCCGCTGCTC 
ATTCCTCTCA GTATGGCTGG ATCAGTCGGG GGTCAGGGTG GTCTGGCGGT GCTCACTTTA 
CCCACCGCCA CTGTGGCCAC TCTCCCCGGT CTCGCTGCTG CCAGTCCTGC TGGAGGCCTG 
CTGAAGCTGC CGTTTGCTGG ACTGCAAGCT GCTACTGTGT TGAACTCTGT GCAGACCCAG 
CTTCAGGCAC CTGCGCAGGC CGTCTTGCAG CCTCAGATGT CTGCTTTACA GGCTATGCAG 
CAGACGCAAA CCACAGCGGC CACCACTGCG TCTATTGTGC AGAAGGCATC AGAGCCCAGT 
GTCTCCGTCG CAACACTGCA GACCGCAGGA CTCTCCATCA ACCCTGCTAT TATTAGTGCA 
GCGTCTTTAG GAGCTCAGCC GCAGTTCATC AGCTCTCTTA CTACGACTCC CATCATCACC 
AGTGCCATGT CAAACGTGGC AGGTCTCACA AGTCAACTCA TCACCAACGC ACAGGGCCAG 
GTGATAGGAA CTCTTCCTCT GCTGGTCAAC CCCGCTTCTT TGGCTGGAGC TGCAGCGGCC 
TCTGCTTTAC CTGCTCAAGG CCTGCAGGTT CAGACCGTTG CTCCACAGCT ACTCCTGAAC 
TCGCAGGGAC AGATCATTGC TACGATAGGG AACGGTCCAA CAGCTGCTAT CCCATCCACC 
GCTTCTGTCC TCCCCAAAGC CACCGTTCCC CTGACCCTCA CCAAAACCAC CACACAGGGT 
CCTGTTGGGA AAGTGGCCCC ATCCAAAGTG ATCATTGCTC CCCAACCGTC AGTGGTTAAA 
CCGGTGACCT CACTCACGGC TGCCGGTGTC ATCGCCTGTG GAGAAATGCC TACCGTCGGG 
CAGCTTGTCA ACAAGCCGTC TGCTGTGAAA GATGAAGAGG CCATTAATCT GGAGGAGATT 
CGAGAGTTCG CCAAGAACTT TAAGATTCGA CGGCTCTCTC TGGGTTTGAC ACAGACTCAA 
GTGGGCCAAG CTCTGACTGC TACCGAGGGC CCTGCGTACA GTCAGTCAGC CATCTGCAGG 
TTTGAGAAGC TGGACATCAC TCCTAAAAGT GCGCAGAAGC TGAAGCCTGT GCTGGAGCGC 
TGGCTGGCCG AGGCAGAGCT CTGGAACCAG AAAGGTCAGC AGAACTTGAT GGAGTTCGTG 
GGAGGAGAAC CGTCCAAGAA ACGCAAACGG AGAACGAGCT TCACGCCACA GGCCATAGAA 
GTCCTCAACA CTTACTTCGA GAAAAACTCC CTTCCGACGG GCCAGGAGAT TACAGAGATC 
GCCAAGGAGC TGAACTACGA CCGAGAGGTG GTTCGGGTCT GGTTCTGCAA CCGTCGACAG 
ACTCTGAAAA ATACCAGCAA GATTAACGTG TTTCAATCCC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208991.1
CDS	674..2398
Translation	MSGQETIRVLEVEVDTALSSAGAAESGGDEEGSGQSLEATEEAQLDGPVTTSSTTAVTVEVSAPVVQTVVSKAAISVSPAQQTSVPITVQACPQVLTQDGLASLMTGMLAQQSSLGQPLLIPLSMAGSVGGQGGLAVLTLPTATVATLPGLAAASPAGGLLKLPFAGLQAATVLNSVQTQLQAPAQAVLQPQMSALQAMQQTQTTAATTASIVQKASEPSVSVATLQTAGLSINPAIISAASLGAQPQFISSLTTTPIITSAMSNVAGLTSQLITNAQGQVIGTLPLLVNPASLAGAAAASALPAQGLQVQTVAPQLLLNSQGQIIATIGNGPTAAIPSTASVLPKATVPLTLTKTTTQGPVGKVAPSKVIIAPQPSVVKPVTSLTAAGVIACGEMPTVGQLVNKPSAVKDEEAINLEEIREFAKNFKIRRLSLGLTQTQVGQALTATEGPAYSQSAICRFEKLDITPKSAQKLKPVLERWLAEAELWNQKGQQNLMEFVGGEPSKKRKRRTSFTPQAIEVLNTYFEKNSLPTGQEITEIAKELNYDREVVRVWFCNRRQTLKNTSKINVFQSQ

Target ORF information:

RefSeq Version	XM_017353502.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio POU class 6 homeobox 1 (pou6f1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353502.1

ORF Insert Sequence:

ATGTCAGGCC AGGAGACCAT CCGTGTGCTG GAGGTGGAGG TGGACACGGC CTTATCTTCT 
GCAGGGGCTG CAGAGTCTGG GGGTGATGAA GAAGGCTCTG GTCAGAGCCT GGAAGCAACA 
GAAGAGGCTC AACTGGACGG TCCAGTAACC ACAAGCTCTA CCACAGCTGT GACTGTGGAG 
GTTTCAGCAC CTGTAGTGCA GACTGTGGTC TCCAAAGCTG CCATCAGTGT GTCTCCAGCT 
CAGCAGACCA GCGTTCCCAT TACTGTTCAG GCTTGCCCAC AGGTCCTGAC TCAGGATGGT 
CTGGCGTCTC TGATGACTGG CATGCTTGCC CAGCAGAGTT CTCTAGGTCA GCCGCTGCTC 
ATTCCTCTCA GTATGGCTGG ATCAGTCGGG GGTCAGGGTG GTCTGGCGGT GCTCACTTTA 
CCCACCGCCA CTGTGGCCAC TCTCCCCGGT CTCGCTGCTG CCAGTCCTGC TGGAGGCCTG 
CTGAAGCTGC CGTTTGCTGG ACTGCAAGCT GCTACTGTGT TGAACTCTGT GCAGACCCAG 
CTTCAGGCAC CTGCGCAGGC CGTCTTGCAG CCTCAGATGT CTGCTTTACA GGCTATGCAG 
CAGACGCAAA CCACAGCGGC CACCACTGCG TCTATTGTGC AGAAGGCATC AGAGCCCAGT 
GTCTCCGTCG CAACACTGCA GACCGCAGGA CTCTCCATCA ACCCTGCTAT TATTAGTGCA 
GCGTCTTTAG GAGCTCAGCC GCAGTTCATC AGCTCTCTTA CTACGACTCC CATCATCACC 
AGTGCCATGT CAAACGTGGC AGGTCTCACA AGTCAACTCA TCACCAACGC ACAGGGCCAG 
GTGATAGGAA CTCTTCCTCT GCTGGTCAAC CCCGCTTCTT TGGCTGGAGC TGCAGCGGCC 
TCTGCTTTAC CTGCTCAAGG CCTGCAGGTT CAGACCGTTG CTCCACAGCT ACTCCTGAAC 
TCGCAGGGAC AGATCATTGC TACGATAGGG AACGGTCCAA CAGCTGCTAT CCCATCCACC 
GCTTCTGTCC TCCCCAAAGC CACCGTTCCC CTGACCCTCA CCAAAACCAC CACACAGGGT 
CCTGTTGGGA AAGTGGCCCC ATCCAAAGTG ATCATTGCTC CCCAACCGTC AGTGGTTAAA 
CCGGTGACCT CACTCACGGC TGCCGGTGTC ATCGCCTGTG GAGAAATGCC TACCGTCGGG 
CAGCTTGTCA ACAAGCCGTC TGCTGTGAAA GATGAAGAGG CCATTAATCT GGAGGAGATT 
CGAGAGTTCG CCAAGAACTT TAAGATTCGA CGGCTCTCTC TGGGTTTGAC ACAGACTCAA 
GTGGGCCAAG CTCTGACTGC TACCGAGGGC CCTGCGTACA GTCAGTCAGC CATCTGCAGG 
TTTGAGAAGC TGGACATCAC TCCTAAAAGT GCGCAGAAGC TGAAGCCTGT GCTGGAGCGC 
TGGCTGGCCG AGGCAGAGCT CTGGAACCAG AAAGGTCAGC AGAACTTGAT GGAGTTCGTG 
GGAGGAGAAC CGTCCAAGAA ACGCAAACGG AGAACGAGCT TCACGCCACA GGCCATAGAA 
GTCCTCAACA CTTACTTCGA GAAAAACTCC CTTCCGACGG GCCAGGAGAT TACAGAGATC 
GCCAAGGAGC TGAACTACGA CCGAGAGGTG GTTCGGGTCT GGTTCTGCAA CCGTCGACAG 
ACTCTGAAAA ATACCAGCAA GATTAACGTG TTTCAATCCC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hypocretin neuron-specific transcriptome profiling identifies the sleep modulator Kcnh4a.
eLife4e08638(2015 Oct)
Yelin-Bekerman L,Elbaz I,Diber A,Dahary D,Gibbs-Bar L,Alon S,Lerer-Goldshtein T,Appelbaum L

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Time point-based integrative analyses of deep-transcriptome identify four signal pathways in blastemal regeneration of zebrafish lower jaw.
Stem cells (Dayton, Ohio)33(3)806-18(2015 Mar)
Zhang H,Wang X,Lyu K,Gao S,Wang G,Fan C,Zhang XA,Yan J

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

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POU6F1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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